Rroxscaffold_2G00098650

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000173
Physical Location & Seq
Forward (+)
20032711 .. 20038362
5652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098650.1

Sequence Viewer

Length: 2040 bp
ATGTGGAGGATCGAGTGTGGAAGGGGTGCAAATGACCCTCATTTATTCAGCACAAACAACTTTCATGGGAGGCAGACATGGAAGTTTGACCCAAATGCAGGAACACCAGAAGAGCGAGAAGAGGTTGAAGCCGCTCGTGAGAATTACTACCAAAATCGGTTCAAGATCCAGCCCAGTAGTGATCTCCTTTGGCGTTTTCAGATGCTAAGAGAAAGAAACTTCAAGCAAGAAATTCCTCCAGTAAGAATTGGGGAAGGTGATGACATTACATTTGATCAGGCCACAGCTGCCTATAGGAGGGCTGCTACCTTCTGGAATGCCTTGCAATCACCCCATGGCCATTGGCCTGCTGAAAATGCTGGCATAAATTTTTTCTGTCCTCCCTTGGTAATGAGTTTATACTCAATGGGATATCTTAATGTTGTTTTCTCTGCTGAGCATAAGAAGGAAATCTTGCGCTACTCGTACAATCATCAGAATGAAGATGGTGGGTGGGGATTGCACATAGCAGGGCCTAGTATGATGTTCACCACATGTCTCAACTACTGTATGATGCGTATCCTCGGAGAAGGCACTGAGGGTGGTCGAGATAATGCCTGTGCAAGGGCCCGGAAATGGATTCTTGACCGCGGTGGTGCACAATATTCTGCTTCATGGGGAAAGACCTGGATGGCGGAAGATAACTACTATCCTCATGGAAGGGTACAACGTTTTATGTGGGACACTCTTTACCATGTTTGTGAGCCTATTCTTGCTTGGTGGCCCTTTAAGAAGATCAGAGACAATGCTATTCAATTTACCATTGACCAAATCCATTATGAAGATGAGAACAGTCGCTACATTACCATTGGATGTATCGAAAAGCCATTAATGATGCTTGCCTGTTGGGTTGAGGATCCTAGCGGAGAAGCTTTTAAGAAGCATCTTCCTAGACTTGATGATTATATTTGGGTCGGTGAAGATGGAATCAAGATTCAGAGTTTCGGCAGCCAGACTTGGGATTGTTCTCTTGCAATCCAAGCTTTGCTTGCTGGGAATCTCCATAATGAATTTGGACCTGTCCTTAAGAAAGCACACGACTTCCTAAAGATATCCCAGGTGAGGATCAATCCTTGTGGTGACCACCTAGCTCATTTCCGTCACATTTCCAAAGGATGCTGGACTTTCTCTGATCGGGATCATGGATGGCAAGTTTCAGATTGCACTGCAGAAGCATTGAGGTGCTGCTGTCTGTTTGCAATGATGTCACCAGAGGTTGTTGGCGACCCAATGGAAGCCGAGTGTATGTATGATGCTGTCGATGTCATAATGTCACTACAGAGTCCAAATGGTGGTGTATCAGCATGGGAGCCGACTGGAGCTCCTAAATGGTTGGAGTGGCTCAACCCTGTCGAATTTTTTGAGGACCTTGTCATTGAATATGATTACGTCGAGTGCACTTCATCTTCGATTCAGGCCTTAGTTTTGTTTAGGAAGTTGTATCCTAATCACAGAAGGAAAGAGATCAACAACTTCATCACAAGGGCTGCAGACTACATTGAAAGCATACAATACGCTGATGGCTCGTGGTATGGAAACTGGGGTATCTGCTTTCTCTATGGAACATGGTTTGCAATCAAAGGGTTAGAGGCTGCACGAAGAACCTACCACAATTGCGAGGCAGTTCGCAAAGGTGTTGACTTTTTGCTAAAAACACAAAGGGAAGATGGTGGTTGGGGAGAGCACTACACCTCATGCACGAACAAGAAATACACAGCTCAAGACAGTACAAATCTGGTGCAAACTGCCCTCGGATTAATGGGTCTAATTCACGGTCGACAGGGTGAGAGAGATCCAACTCCTATTCACCGTGCTGCAAGGGTCTTGATGAACGGTCATTTGGATGATGGTGATTTTCCCCAACAGGAACTGGTGGGAGTTTTCATGAGGAATGCAATGTTACACTATGGAGCATATAGGAATGTTTTCCCATTGTGGGCTCTTGGAGAGTATCGTACGTACTCTGGAGCGGTTGCTTTAGCAAAAGGATTTGAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

679

Amino Acids

78.02

Weight (kDa)

5.9

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 101 - 232 1.3e-12 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 330 - 665 6.8e-47 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1331
AccBSI CCGCTC 2 cut(s) 134, 2009
AccI GTMKAC 1 cut(s) 1816
AccII CGCG 1 cut(s) 630
AciI CCGC 6 cut(s) 132, 628, 630, 674, 903, 2009
AclI AACGTT 1 cut(s) 709
AclWI GGATC 7 cut(s) 17, 160, 890, 903, 1112, 1185, 1826
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 4 cut(s) 231, 367, 1049, 1394
AfaI GTAC 5 cut(s) 467, 705, 1768, 1996, 2000
AfiI CCNNNNNNNGG 8 cut(s) 98, 297, 603, 615, 997, 1101, 1331, 1975
AflII CTTAAG 1 cut(s) 1064
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 7 cut(s) 128, 163, 223, 794, 1418, 1541, 2033
AjnI CCWGG 2 cut(s) 665, 1095
AjuI GAANNNNNNNTTGG 2 cut(s) 1862, 1894
AluBI AGCT 6 cut(s) 287, 911, 1022, 1130, 1361, 1757
AluI AGCT 6 cut(s) 287, 911, 1022, 1130, 1361, 1757
Alw21I GWGCWC 4 cut(s) 640, 1363, 1439, 1725
Alw26I GTCTC 2 cut(s) 542, 774
Alw44I GTGCAC 2 cut(s) 636, 1435
AlwI GGATC 7 cut(s) 17, 160, 890, 903, 1112, 1185, 1826
AlwNI CAGNNNCTG 1 cut(s) 1909
AoxI GGCC 7 cut(s) 279, 337, 344, 512, 606, 761, 1455
ApaI GGGCCC 1 cut(s) 610
ApaLI GTGCAC 2 cut(s) 636, 1435
ApeKI GCWGC 7 cut(s) 287, 302, 987, 1224, 1526, 1631, 1853
ApoI RAATTY 4 cut(s) 231, 367, 1049, 1394
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AseI ATTAAT 2 cut(s) 869, 1796
Asp700I GAANNNNTTC 1 cut(s) 1964
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 6 cut(s) 512, 606, 607, 762, 1055, 1405
AsuC2I CCSGG 1 cut(s) 610
AvaII GGWCC 2 cut(s) 1055, 1405
BaeGI GKGCMC 3 cut(s) 610, 640, 1439
BaeI ACNNNNGTAYC 2 cut(s) 1567, 1600
BalI TGGCCA 1 cut(s) 339
BamHI GGATCC 1 cut(s) 895
BanII GRGCYC 3 cut(s) 610, 1363, 1981
BauI CACGAG 2 cut(s) 135, 1564
Bbv12I GWGCWC 4 cut(s) 640, 1363, 1439, 1725
BbvI GCAGC 7 cut(s) 274, 289, 999, 1211, 1513, 1618, 1840
BccI CCATC 7 cut(s) 479, 664, 956, 1179, 1553, 1700, 1880
BciT130I CCWGG 2 cut(s) 667, 1097
BciVI GTATCC 2 cut(s) 569, 1491
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 2 cut(s) 542, 774
BfaI CTAG 4 cut(s) 516, 900, 930, 1127
BfmI CTRYAG 4 cut(s) 292, 1206, 1316, 1527
BfrI CTTAAG 1 cut(s) 1064
BfuI GTATCC 2 cut(s) 569, 1491
BisI GCNGC 8 cut(s) 132, 288, 303, 988, 1225, 1527, 1632, 1854
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 8 cut(s) 133, 289, 304, 989, 1226, 1528, 1633, 1855
Bme1390I CCNGG 3 cut(s) 610, 667, 1097
Bme18I GGWCC 2 cut(s) 1055, 1405
BmgT120I GGNCC 6 cut(s) 512, 606, 607, 762, 1055, 1405
BmiI GGNNCC 3 cut(s) 608, 897, 1350
BmrFI CCNGG 3 cut(s) 610, 667, 1097
BmrI ACTGGG 2 cut(s) 168, 1588
BmsI GCATC 6 cut(s) 192, 543, 864, 931, 1145, 1282
BmuI ACTGGG 2 cut(s) 168, 1588
BpmI CTGGAG 3 cut(s) 222, 1377, 2025
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 1743
BpuMI CCSGG 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 1998
BsaBI GATNNNNATC 2 cut(s) 557, 1176
BsaJI CCNNGG 6 cut(s) 334, 384, 562, 628, 1095, 1789
BsaXI ACNNNNNCTCC 2 cut(s) 1345, 1375
Bsc4I CCNNNNNNNGG 8 cut(s) 98, 297, 603, 615, 997, 1101, 1331, 1975
Bse1I ACTGG 5 cut(s) 174, 239, 1360, 1583, 1914
Bse3DI GCAATG 2 cut(s) 1245, 1941
Bse8I GATNNNNATC 2 cut(s) 557, 1176
BseBI CCWGG 2 cut(s) 667, 1097
BseDI CCNNGG 6 cut(s) 334, 384, 562, 628, 1095, 1789
BseGI GGATG 5 cut(s) 675, 857, 1160, 1190, 1888
BseJI GATNNNNATC 2 cut(s) 557, 1176
BseLI CCNNNNNNNGG 8 cut(s) 98, 297, 603, 615, 997, 1101, 1331, 1975
BseMI GCAATG 2 cut(s) 1245, 1941
BseMII CTCAG 2 cut(s) 426, 567
BseNI ACTGG 5 cut(s) 174, 239, 1360, 1583, 1914
BseSI GKGCMC 3 cut(s) 610, 640, 1439
BseXI GCAGC 7 cut(s) 274, 289, 999, 1211, 1513, 1618, 1840
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 1617
Bsh1236I CGCG 1 cut(s) 630
Bsh1285I CGRYCG 1 cut(s) 1816
BshFI GGCC 7 cut(s) 281, 339, 346, 514, 608, 763, 1457
BsiEI CGRYCG 1 cut(s) 1816
BsiHKAI GWGCWC 4 cut(s) 640, 1363, 1439, 1725
BsiSI CCGG 1 cut(s) 610
BsiWI CGTACG 1 cut(s) 1994
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 8 cut(s) 98, 297, 603, 615, 997, 1101, 1331, 1975
BsmAI GTCTC 2 cut(s) 542, 774
BsmFI GGGAC 1 cut(s) 734
BsmI GAATGC 2 cut(s) 322, 1936
BsnI GGCC 7 cut(s) 281, 339, 346, 514, 608, 763, 1457
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 6 cut(s) 610, 640, 1363, 1439, 1725, 1981
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 6 cut(s) 132, 628, 630, 674, 903, 2009
BspANI GGCC 7 cut(s) 281, 339, 346, 514, 608, 763, 1457
BspCNI CTCAG 2 cut(s) 427, 568
BspFNI CGCG 1 cut(s) 630
BspHI TCATGA 1 cut(s) 1923
BspLI GGNNCC 3 cut(s) 608, 897, 1350
BspMAI CTGCAG 2 cut(s) 1210, 1531
BspPI GGATC 7 cut(s) 17, 160, 890, 903, 1112, 1185, 1826
BspQI GCTCTTC 1 cut(s) 105
BspTI CTTAAG 1 cut(s) 1064
BsrBI CCGCTC 2 cut(s) 134, 2009
BsrDI GCAATG 2 cut(s) 1245, 1941
BsrI ACTGG 5 cut(s) 174, 239, 1360, 1583, 1914
BssECI CCNNGG 6 cut(s) 334, 384, 562, 628, 1095, 1789
BssSI CACGAG 2 cut(s) 135, 1564
BssT1I CCWWGG 2 cut(s) 334, 384
Bst2BI CACGAG 2 cut(s) 135, 1564
Bst2UI CCWGG 2 cut(s) 667, 1097
Bst4CI ACNGT 6 cut(s) 548, 833, 1766, 1814, 1850, 1874
Bst6I CTCTTC 2 cut(s) 105, 114
BstAFI CTTAAG 1 cut(s) 1064
BstBAI YACGTR 1 cut(s) 1998
BstC8I GCNNGC 4 cut(s) 348, 361, 879, 1029
BstDEI CTNAG 4 cut(s) 206, 435, 576, 1459
BstDSI CCRYGG 2 cut(s) 334, 628
BstEII GGTNACC 1 cut(s) 1118
BstENI CCTNNNNNAGG 2 cut(s) 295, 601
BstF5I GGATG 5 cut(s) 675, 857, 1160, 1190, 1888
BstFNI CGCG 1 cut(s) 630
BstHHI GCGC 1 cut(s) 459
BstMAI GTCTC 2 cut(s) 542, 774
BstMCI CGRYCG 1 cut(s) 1816
BstMWI GCNNNNNNNGC 4 cut(s) 287, 356, 1019, 1028
BstNI CCWGG 2 cut(s) 667, 1097
BstNSI RCATGY 1 cut(s) 537
BstPI GGTNACC 1 cut(s) 1118
BstSCI CCNGG 3 cut(s) 608, 665, 1095
BstSFI CTRYAG 4 cut(s) 292, 1206, 1316, 1527
BstSLI GKGCMC 3 cut(s) 610, 640, 1439
BstSNI TACGTA 1 cut(s) 1998
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 7 cut(s) 274, 289, 999, 1211, 1513, 1618, 1840
BstX2I RGATCY 3 cut(s) 165, 895, 1831
BstYI RGATCY 3 cut(s) 165, 895, 1831
BsuI GTATCC 2 cut(s) 569, 1491
BsuRI GGCC 7 cut(s) 281, 339, 346, 514, 608, 763, 1457
BtgI CCRYGG 2 cut(s) 334, 628
BtsCI GGATG 5 cut(s) 675, 857, 1160, 1190, 1888
BtsI GCAGTG 1 cut(s) 1203
BtsIMutI CAGTG 2 cut(s) 573, 1203
Cac8I GCNNGC 4 cut(s) 348, 361, 879, 1029
CaiI CAGNNNCTG 1 cut(s) 1909
CciI TCATGA 1 cut(s) 1923
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 6 cut(s) 512, 606, 607, 762, 1055, 1405
Cfr42I CCGCGG 1 cut(s) 631
Csp6I GTAC 5 cut(s) 466, 704, 1767, 1995, 1999
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
CviQI GTAC 5 cut(s) 466, 704, 1767, 1995, 1999
DdeI CTNAG 4 cut(s) 206, 435, 576, 1459
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 105, 114
EarI CTCTTC 2 cut(s) 105, 114
EciI GGCGGA 1 cut(s) 689
Ecl136II GAGCTC 1 cut(s) 1361
Eco105I TACGTA 1 cut(s) 1998
Eco130I CCWWGG 2 cut(s) 334, 384
Eco147I AGGCCT 1 cut(s) 1457
Eco24I GRGCYC 3 cut(s) 610, 1363, 1981
Eco32I GATATC 2 cut(s) 413, 1092
Eco47I GGWCC 2 cut(s) 1055, 1405
Eco53kI GAGCTC 1 cut(s) 1361
Eco91I GGTNACC 1 cut(s) 1118
EcoICRI GAGCTC 1 cut(s) 1361
EcoNI CCTNNNNNAGG 2 cut(s) 295, 601
EcoO109I RGGNCCY 3 cut(s) 512, 606, 1405
EcoO65I GGTNACC 1 cut(s) 1118
EcoRII CCWGG 2 cut(s) 665, 1095
EcoRV GATATC 2 cut(s) 413, 1092
EcoT14I CCWWGG 2 cut(s) 334, 384
EcoT38I GRGCYC 3 cut(s) 610, 1363, 1981
ErhI CCWWGG 2 cut(s) 334, 384
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1011, 1043
FaqI GGGAC 1 cut(s) 734
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 1 cut(s) 1816
Fnu4HI GCNGC 8 cut(s) 132, 288, 303, 988, 1225, 1527, 1632, 1854
FokI GGATG 5 cut(s) 682, 864, 1167, 1197, 1895
FriOI GRGCYC 3 cut(s) 610, 1363, 1981
Fsp4HI GCNGC 8 cut(s) 132, 288, 303, 988, 1225, 1527, 1632, 1854
FspBI CTAG 4 cut(s) 516, 900, 930, 1127
GlaI GCGC 1 cut(s) 458
GluI GCNGC 8 cut(s) 132, 288, 303, 988, 1225, 1527, 1632, 1854
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 3 cut(s) 222, 1377, 2025
HaeIII GGCC 7 cut(s) 281, 339, 346, 514, 608, 763, 1457
HapII CCGG 1 cut(s) 610
HhaI GCGC 1 cut(s) 459
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 2 cut(s) 1678, 1817
HindII GTYRAC 2 cut(s) 1678, 1817
HindIII AAGCTT 2 cut(s) 909, 1020
HinfI GANTC 6 cut(s) 619, 966, 973, 1036, 1321, 1450
HpaII CCGG 1 cut(s) 610
Hpy166II GTNNAC 5 cut(s) 528, 638, 1437, 1678, 1817
Hpy188I TCNGA 8 cut(s) 201, 477, 566, 779, 978, 1171, 1198, 1793
Hpy8I GTNNAC 5 cut(s) 528, 638, 1437, 1678, 1817
Hpy99I CGWCG 1 cut(s) 1433
HpyAV CCTTC 7 cut(s) 15, 248, 319, 439, 563, 693, 1488
HpyCH4III ACNGT 6 cut(s) 548, 833, 1766, 1814, 1850, 1874
HpyCH4IV ACGT 3 cut(s) 709, 1428, 1997
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 356, 1019, 1028
HpyF3I CTNAG 4 cut(s) 206, 435, 576, 1459
HpySE526I ACGT 3 cut(s) 709, 1428, 1997
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 274
KspI CCGCGG 1 cut(s) 631
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 5 cut(s) 1348, 1358, 1366, 1949, 2006
Lsp1109I GCAGC 7 cut(s) 274, 289, 999, 1211, 1513, 1618, 1840
LweI GCATC 6 cut(s) 192, 543, 864, 931, 1145, 1282
MaeI CTAG 4 cut(s) 516, 900, 930, 1127
MaeII ACGT 3 cut(s) 709, 1428, 1997
MaeIII GTNAC 5 cut(s) 1118, 1139, 1245, 1311, 1938
MbiI CCGCTC 2 cut(s) 134, 2009
MfeI CAATTG 1 cut(s) 1651
MflI RGATCY 3 cut(s) 165, 895, 1831
MhlI GDGCHC 6 cut(s) 610, 640, 1363, 1439, 1725, 1981
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 9 cut(s) 142, 231, 246, 367, 794, 1049, 1394, 1651, 1806
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 1 cut(s) 1330
MmeI TCCRAC 2 cut(s) 1353, 1859
Mox20I TGGCCA 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 1964
MscI TGGCCA 1 cut(s) 339
MseI TTAA 6 cut(s) 417, 768, 869, 915, 1065, 1796
MslI CAYNNNNRTG 4 cut(s) 477, 738, 1219, 1881
Msp20I TGGCCA 1 cut(s) 339
MspA1I CMGCKG 2 cut(s) 287, 630
MspCI CTTAAG 1 cut(s) 1064
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 3 cut(s) 610, 667, 1097
MunI CAATTG 1 cut(s) 1651
Mva1269I GAATGC 2 cut(s) 322, 1936
MvaI CCWGG 2 cut(s) 667, 1097
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 4 cut(s) 287, 356, 1019, 1028
NciI CCSGG 1 cut(s) 610
NcoI CCATGG 1 cut(s) 334
NlaIV GGNNCC 3 cut(s) 608, 897, 1350
NmeAIII GCCGAG 1 cut(s) 1303
NmuCI GTSAC 4 cut(s) 1118, 1139, 1245, 1311
NspI RCATGY 1 cut(s) 537
PagI TCATGA 1 cut(s) 1923
PceI AGGCCT 1 cut(s) 1457
PciI ACATGT 1 cut(s) 533
PciSI GCTCTTC 1 cut(s) 105
PctI GAATGC 2 cut(s) 322, 1936
PdmI GAANNNNTTC 1 cut(s) 1964
PfeI GAWTC 5 cut(s) 619, 966, 973, 1036, 1450
Pfl23II CGTACG 1 cut(s) 1994
PflFI GACNNNGTC 1 cut(s) 1409
PflMI CCANNNNNTGG 1 cut(s) 1331
PkrI GCNGC 8 cut(s) 133, 289, 304, 989, 1226, 1528, 1633, 1855
PleI GAGTC 1 cut(s) 1329
PpsI GAGTC 1 cut(s) 1329
Ppu21I YACGTR 1 cut(s) 1998
PpuMI RGGWCCY 1 cut(s) 1405
PscI ACATGT 1 cut(s) 533
PshBI ATTAAT 2 cut(s) 869, 1796
Psp124BI GAGCTC 1 cut(s) 1363
Psp1406I AACGTT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 1405
Psp6I CCWGG 2 cut(s) 665, 1095
PspEI GGTNACC 1 cut(s) 1118
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 2 cut(s) 665, 1095
PspLI CGTACG 1 cut(s) 1994
PspN4I GGNNCC 3 cut(s) 608, 897, 1350
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 6 cut(s) 512, 606, 607, 762, 1055, 1405
PspPPI RGGWCCY 1 cut(s) 1405
PsrI GAACNNNNNNTAC 4 cut(s) 821, 853, 1733, 1765
PstI CTGCAG 2 cut(s) 1210, 1531
PstNI CAGNNNCTG 1 cut(s) 1909
PsuI RGATCY 3 cut(s) 165, 895, 1831
PsyI GACNNNGTC 1 cut(s) 1409
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 5 cut(s) 467, 705, 1768, 1996, 2000
RsaNI GTAC 5 cut(s) 466, 704, 1767, 1995, 1999
RseI CAYNNNNRTG 4 cut(s) 477, 738, 1219, 1881
SacI GAGCTC 1 cut(s) 1363
SacII CCGCGG 1 cut(s) 631
SalI GTCGAC 1 cut(s) 1815
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 6 cut(s) 417, 768, 869, 915, 1065, 1796
SatI GCNGC 8 cut(s) 132, 288, 303, 988, 1225, 1527, 1632, 1854
Sau96I GGNCC 6 cut(s) 512, 606, 607, 762, 1055, 1405
SchI GAGTC 1 cut(s) 1330
ScrFI CCNGG 3 cut(s) 610, 667, 1097
SduI GDGCHC 6 cut(s) 610, 640, 1363, 1439, 1725, 1981
SfaNI GCATC 6 cut(s) 192, 543, 864, 931, 1145, 1282
SfcI CTRYAG 4 cut(s) 292, 1206, 1316, 1527
Sfr303I CCGCGG 1 cut(s) 631
SgrBI CCGCGG 1 cut(s) 631
SinI GGWCC 2 cut(s) 1055, 1405
SmiMI CAYNNNNRTG 4 cut(s) 477, 738, 1219, 1881
SmlI CTYRAG 2 cut(s) 1064, 1758
SmoI CTYRAG 2 cut(s) 1064, 1758
SnaBI TACGTA 1 cut(s) 1998
Sse9I AATT 9 cut(s) 142, 231, 246, 367, 794, 1049, 1394, 1651, 1806
SseBI AGGCCT 1 cut(s) 1457
SsiI CCGC 6 cut(s) 132, 628, 630, 674, 903, 2009
SspI AATATT 1 cut(s) 644
SspMI CTAG 4 cut(s) 516, 900, 930, 1127
SstI GAGCTC 1 cut(s) 1363
StuI AGGCCT 1 cut(s) 1457
StyD4I CCNGG 3 cut(s) 608, 665, 1095
StyI CCWWGG 2 cut(s) 334, 384
TaaI ACNGT 6 cut(s) 548, 833, 1766, 1814, 1850, 1874
TaiI ACGT 3 cut(s) 712, 1431, 2000
TaqI TCGA 8 cut(s) 12, 586, 858, 1299, 1392, 1431, 1448, 1816
TasI AATT 9 cut(s) 142, 231, 246, 367, 794, 1049, 1394, 1651, 1806
TatI WGTACW 1 cut(s) 1766
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 5 cut(s) 619, 966, 973, 1036, 1450
Tru1I TTAA 6 cut(s) 417, 768, 869, 915, 1065, 1796
Tru9I TTAA 6 cut(s) 417, 768, 869, 915, 1065, 1796
TscAI CASTG 2 cut(s) 580, 1210
TseFI GTSAC 4 cut(s) 1118, 1139, 1245, 1311
TseI GCWGC 7 cut(s) 287, 302, 987, 1224, 1526, 1631, 1853
Tsp45I GTSAC 4 cut(s) 1118, 1139, 1245, 1311
TspDTI ATGAA 9 cut(s) 53, 495, 642, 834, 1062, 1431, 1504, 1883, 1912
TspGWI ACGGA 1 cut(s) 1127
TspRI CASTG 2 cut(s) 580, 1210
Tth111I GACNNNGTC 1 cut(s) 1409
Van91I CCANNNNNTGG 1 cut(s) 1331
Vha464I CTTAAG 1 cut(s) 1064
VneI GTGCAC 2 cut(s) 636, 1435
VpaK11BI GGWCC 2 cut(s) 1055, 1405
VspI ATTAAT 2 cut(s) 869, 1796
XagI CCTNNNNNAGG 2 cut(s) 295, 601
XapI RAATTY 4 cut(s) 231, 367, 1049, 1394
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 1049
XmiI GTMKAC 1 cut(s) 1816
XmnI GAANNNNTTC 1 cut(s) 1964
XspI CTAG 4 cut(s) 516, 900, 930, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.