Rroxscaffold_2G00098740

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000173
Physical Location & Seq
Reverse (-)
20168339 .. 20174518
6180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098740.1

Sequence Viewer

Length: 2331 bp
ATGTGGAAGCTTAGGTTTGGAGACGAAGGTGCAAATAATGACCCTTACTTGTTTAGCACAAACAATTTCCATGGGAGGCAAACATGGGAGTTCGACCCGGCTGCCGGCACAACTGAAGAGCGAGCAAAGGTTGAAAAAGTTCGGCTAAGTTTTTACAACAATCGTCACCGGGTCCAGGCTTGTAGTGACCTCCTCTGTCGAATGCAGGTGCTTCTGGGAGAGAAAAAGTTCAAACAAAAGATTCCCCAAGTAAAGGTGGAGGATGGTGAGGACTGTACATATGAAAAGGCCACCGCTGCATTTAGAAGGGCAGCTACCTTCTACTCTGCCTTGCAATCACCTCACGGCCATTGGCCTGCTGAAAATGCCGGTCCCATGTTTTATATTCCTCCCTTGGTCATATCGCTCTATATTACGGGGCATCTTAGTAAGGTATTCTCGGCAGAGCATTTTAAGGAAATGCTTAGATATATATATTGTCATCAGAACGAGGATGGTGGGTGGGGATTACACATACAGGCCCCTAGTATGATGTTTTGTACACTTCTGAATTACATTTGCATGCGTCTACTTGGAGAAGGACCCGATGGTGGTTTAAACAATTCTTGTGCAAGAGCACGCAAGTGGATTCTTGATCATGGTGGTGCAACACATTCATCATCCTGGGGAAAGACTTATATGGCAATACTTGGTGTGTATGAGTGGGAGGGATGCAACCCGATTCCCCCAGAGTTTTGGATTTTCCCGTCTTTGTTTCCTTTGCATCCAGCAAAAATGTACTGCTACTGCCGGTTGACTTACTTGCCTTTATCGTATCTGTTTGGGAAAAGATTTGTTGGCCCCATCACCCCTCTCATTCTACAACTGAGAGAAGAAATGTACAATGAGCCATACAATGAAATTCAATGGGGCAAAGTACGCCATCTCTGTGCAAAGGAGGATAACTACTATCCCCATGGCAAGTTTCAATGTTTGATGTGGGATACTCTGAACACGGTTTATGAGCCTCTTCTTTCTTGCTGGCCCTTCAATAAGTTGAGAGAGAAGGCTCTTCAAGAAACCATTGAACACATTCATTATGAAGATGAAAATAGTCGATACATAACAATTGGATGTGGGGAAAAGCCATTAATGATGCTTGCTTGCTGGATTGAAGATCCGAATGGAGAACCCTTTAAGAAGCATCTTGCAAGAGTTATAGATTACATATGGGTTGCAGAAGATGGAATAACTACGCAGAGTTTTGGTAGCCAGGCATGGGATGCTAGTCTTTCTGTTCAAGCTTTGCTTGATGGAAATCTCACTGATGAACTTGGATCTGTACTTCAGAAAGCACATGAATTCCTAAACAAATCTCAGGTCTCCATGCTTAATGAGTCCGACAAGCCTCCTCTTCTTATTAGGGATAATCCTTCTGGTGACTTCTTATCTCGGTTCCGTCACATTTCTAAAGGTGGTTGGACTTTCTCTGACCGAGATCATGGATGGCAAGTTTCTGATTGTACTGCAGAGTGTTTGAAGTGTTGCCTATTGTTTTCATTGATGCCGACGCAAATCGTGGGTGAACCAATTGAATCTGAGCGCTTGTATGATGCTGTCAATGTCATACTTTCTCTACAAAATGCAAATGGTGGTGTATCAGCCTGGCAGCCAACAGGAGCTCCACAATGGTTGGAGTGGTTCAATCCAATAGAATTTCTCGGGGATGTCGTCATTGAATATGAGTATGTTGAGTGCACTTCATCTTCAATTCAAGCGTTAGCTTTATTCAGGAAGTTATACCCTGGGCATAGAAAGAAACATATCGATGATTTCATCACAAATGCAGCATGTTACCTTGAAGACATGCAGACACTTGATGGCTCATGGTATGGAAGATGGGGAATCTGCTTCATCTATGGAACATGGTTTGCAATCCGAGGCTTGAAGGCTGCTGGTAGAACTTACAAAAATTGTGAGGCGATACGCAGAGGTGTTGAATTTTTACTCAAAATACAGAGAAATGATGGTGGTTGGGGAGAGAGCTATACTTCTTGTACAAACAAGATCTATACACCTCTTGAAGGCGACCGTTCGAATTTGGTGCAGACTGCAATGGGACTAATGGGATTAATTCATGGTGGACAGGCCGAGAGAGACCCTACTCCCATTCACAGAGCTGCGAAGCTTTTGATTAATTCTCAATTGGAAAATGGAGATTTCCCCCAACAGGAACTGATGGGGGTTTTCAATGTCAATGCCATGTTACACTATGCAGCATACAGGAATATCTTCCCAATTTGGGCTCTCGGAGAATATCGTTCCCTTCCCTCCTTCTGGATAGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

776

Amino Acids

88.88

Weight (kDa)

5.97

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 103 - 363 3.3e-36 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 417 - 767 1.1e-43 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 568
AciI CCGC 1 cut(s) 294
AclWI GGATC 2 cut(s) 1151, 1324
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 5 cut(s) 900, 1340, 1694, 1979, 2077
AcuI CTGAAG 2 cut(s) 135, 1310
AfaI GTAC 8 cut(s) 277, 541, 779, 881, 918, 1323, 1504, 2038
AfeI AGCGCT 1 cut(s) 1583
AfiI CCNNNNNNNGG 9 cut(s) 104, 175, 253, 590, 1258, 2063, 2209, 2281, 2316
AjnI CCWGG 5 cut(s) 174, 662, 1251, 1643, 1783
AleI CACNNNNGTG 1 cut(s) 622
AluBI AGCT 8 cut(s) 10, 314, 1283, 1661, 1763, 2025, 2159, 2167
AluI AGCT 8 cut(s) 10, 314, 1283, 1661, 1763, 2025, 2159, 2167
Alw21I GWGCWC 3 cut(s) 619, 1663, 1739
Alw26I GTCTC 3 cut(s) 15, 1366, 2130
Alw44I GTGCAC 1 cut(s) 1735
AlwI GGATC 2 cut(s) 1151, 1324
AlwNI CAGNNNCTG 1 cut(s) 2215
Ama87I CYCGRG 1 cut(s) 1700
Aor51HI AGCGCT 1 cut(s) 1583
AoxI GGCC 7 cut(s) 288, 346, 353, 519, 838, 1022, 2127
ApaLI GTGCAC 1 cut(s) 1735
ApeKI GCWGC 8 cut(s) 101, 296, 311, 1648, 1826, 1931, 2159, 2255
ApoI RAATTY 5 cut(s) 900, 1340, 1694, 1979, 2077
ArsI GACNNNNNNTTYG 2 cut(s) 1344, 1376
AseI ATTAAT 3 cut(s) 1130, 2111, 2175
Asp700I GAANNNNTTC 4 cut(s) 138, 227, 1071, 2270
AspLEI GCGC 1 cut(s) 1584
AspS9I GGNCC 6 cut(s) 172, 371, 520, 581, 839, 1023
AsuC2I CCSGG 2 cut(s) 98, 170
AsuHPI GGTGA 6 cut(s) 158, 278, 330, 838, 1430, 1574
AsuII TTCGAA 1 cut(s) 2075
AvaI CYCGRG 1 cut(s) 1700
AvaII GGWCC 3 cut(s) 172, 371, 581
BaeGI GKGCMC 1 cut(s) 1739
BanII GRGCYC 2 cut(s) 1663, 2287
BarI GAAGNNNNNNTAC 2 cut(s) 298, 330
BbsI GAAGAC 1 cut(s) 1848
Bbv12I GWGCWC 3 cut(s) 619, 1663, 1739
BbvI GCAGC 8 cut(s) 88, 283, 323, 1660, 1838, 1918, 2146, 2267
BceAI ACGGC 1 cut(s) 361
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 2065, 2099
BciT130I CCWGG 5 cut(s) 176, 664, 1253, 1645, 1785
BciVI GTATCC 1 cut(s) 976
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 2 cut(s) 98, 170
BcoDI GTCTC 3 cut(s) 15, 1366, 2130
BfaI CTAG 2 cut(s) 525, 1266
BfmI CTRYAG 1 cut(s) 1506
BfoI RGCGCY 1 cut(s) 1585
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 976
BglII AGATCT 1 cut(s) 2046
BisI GCNGC 8 cut(s) 102, 297, 312, 1649, 1827, 1932, 2160, 2256
BlsI GCNGC 8 cut(s) 103, 298, 313, 1650, 1828, 1933, 2161, 2257
Bme1390I CCNGG 7 cut(s) 98, 170, 176, 664, 1253, 1645, 1785
Bme18I GGWCC 3 cut(s) 172, 371, 581
BmeT110I CYCGRG 1 cut(s) 1700
BmgT120I GGNCC 6 cut(s) 172, 371, 520, 581, 839, 1023
BmiI GGNNCC 6 cut(s) 173, 373, 522, 583, 841, 1436
BmrFI CCNGG 7 cut(s) 98, 170, 176, 664, 1253, 1645, 1785
BmsI GCATC 8 cut(s) 430, 701, 772, 1125, 1192, 1252, 1533, 1582
BpiI GAAGAC 1 cut(s) 1848
Bpu10I CCTNAGC 1 cut(s) 11
Bpu14I TTCGAA 1 cut(s) 2075
BpuMI CCSGG 2 cut(s) 98, 170
Bsa29I ATCGAT 1 cut(s) 1806
BsaBI GATNNNNATC 1 cut(s) 1296
BsaI GGTCTC 2 cut(s) 1366, 2130
BsaJI CCNNGG 7 cut(s) 70, 393, 663, 955, 1783, 1784, 1918
BsaXI ACNNNNNCTCC 2 cut(s) 1645, 1675
Bsc4I CCNNNNNNNGG 9 cut(s) 104, 175, 253, 590, 1258, 2063, 2209, 2281, 2316
Bse118I RCCGGY 3 cut(s) 104, 368, 789
Bse3DI GCAATG 1 cut(s) 2100
Bse8I GATNNNNATC 1 cut(s) 1296
BseBI CCWGG 5 cut(s) 176, 664, 1253, 1645, 1785
BseCI ATCGAT 1 cut(s) 1806
BseDI CCNNGG 7 cut(s) 70, 393, 663, 955, 1783, 1784, 1918
BseGI GGATG 9 cut(s) 268, 499, 659, 716, 763, 1118, 1267, 1490, 1711
BseJI GATNNNNATC 1 cut(s) 1296
BseLI CCNNNNNNNGG 9 cut(s) 104, 175, 253, 590, 1258, 2063, 2209, 2281, 2316
BseMI GCAATG 1 cut(s) 2100
BseMII CTCAG 3 cut(s) 857, 1370, 1569
BseRI GAGGAG 2 cut(s) 182, 1380
BseSI GKGCMC 1 cut(s) 1739
BseXI GCAGC 8 cut(s) 88, 283, 323, 1660, 1838, 1918, 2146, 2267
BsgI GTGCAG 1 cut(s) 2105
Bsh1285I CGRYCG 1 cut(s) 2071
BshFI GGCC 7 cut(s) 290, 348, 355, 521, 840, 1024, 2129
BshVI ATCGAT 1 cut(s) 1806
BsiEI CGRYCG 1 cut(s) 2071
BsiHKAI GWGCWC 3 cut(s) 619, 1663, 1739
BsiHKCI CYCGRG 1 cut(s) 1700
BsiSI CCGG 5 cut(s) 98, 105, 169, 369, 790
BslFI GGGAC 2 cut(s) 357, 2112
BslI CCNNNNNNNGG 9 cut(s) 104, 175, 253, 590, 1258, 2063, 2209, 2281, 2316
BsmAI GTCTC 3 cut(s) 15, 1366, 2130
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 357, 2112
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 7 cut(s) 290, 348, 355, 521, 840, 1024, 2129
Bso31I GGTCTC 2 cut(s) 1366, 2130
BsoBI CYCGRG 1 cut(s) 1700
Bsp119I TTCGAA 1 cut(s) 2075
Bsp1286I GDGCHC 4 cut(s) 619, 1663, 1739, 2287
Bsp1407I TGTACA 4 cut(s) 275, 539, 879, 2036
Bsp143I GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
Bsp19I CCATGG 2 cut(s) 70, 955
BspACI CCGC 1 cut(s) 294
BspANI GGCC 7 cut(s) 290, 348, 355, 521, 840, 1024, 2129
BspCNI CTCAG 3 cut(s) 858, 1369, 1570
BspDI ATCGAT 1 cut(s) 1806
BspLI GGNNCC 6 cut(s) 173, 373, 522, 583, 841, 1436
BspMAI CTGCAG 1 cut(s) 1510
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 2 cut(s) 1151, 1324
BspQI GCTCTTC 2 cut(s) 111, 1056
BspT104I TTCGAA 1 cut(s) 2075
BspTNI GGTCTC 2 cut(s) 1366, 2130
BsrDI GCAATG 1 cut(s) 2100
BsrFI RCCGGY 3 cut(s) 104, 368, 789
BsrGI TGTACA 4 cut(s) 275, 539, 879, 2036
BssAI RCCGGY 3 cut(s) 104, 368, 789
BssECI CCNNGG 7 cut(s) 70, 393, 663, 955, 1783, 1784, 1918
BssMI GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
BssT1I CCWWGG 3 cut(s) 70, 393, 955
Bst2UI CCWGG 5 cut(s) 176, 664, 1253, 1645, 1785
Bst4CI ACNGT 3 cut(s) 275, 997, 2072
Bst6I CTCTTC 4 cut(s) 111, 1014, 1056, 1398
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstAUI TGTACA 4 cut(s) 275, 539, 879, 2036
BstBI TTCGAA 1 cut(s) 2075
BstC8I GCNNGC 8 cut(s) 106, 123, 357, 563, 619, 1022, 1140, 1144
BstDEI CTNAG 7 cut(s) 11, 146, 425, 464, 866, 1356, 1578
BstDSI CCRYGG 2 cut(s) 70, 955
BstENI CCTNNNNNAGG 1 cut(s) 2061
BstF5I GGATG 9 cut(s) 268, 499, 659, 716, 763, 1118, 1267, 1490, 1711
BstH2I RGCGCY 1 cut(s) 1585
BstHHI GCGC 1 cut(s) 1584
BstKTI GATC 5 cut(s) 637, 1159, 1319, 1480, 2049
BstMAI GTCTC 3 cut(s) 15, 1366, 2130
BstMBI GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
BstMCI CGRYCG 1 cut(s) 2071
BstMWI GCNNNNNNNGC 4 cut(s) 296, 365, 918, 1262
BstNI CCWGG 5 cut(s) 176, 664, 1253, 1645, 1785
BstNSI RCATGY 3 cut(s) 565, 1833, 1849
BstSCI CCNGG 7 cut(s) 96, 168, 174, 662, 1251, 1643, 1783
BstSFI CTRYAG 1 cut(s) 1506
BstSLI GKGCMC 1 cut(s) 1739
BstV1I GCAGC 8 cut(s) 88, 283, 323, 1660, 1838, 1918, 2146, 2267
BstV2I GAAGAC 1 cut(s) 1848
BstX2I RGATCY 3 cut(s) 1156, 1316, 2046
BstXI CCANNNNNNTGG 1 cut(s) 735
BstYI RGATCY 3 cut(s) 1156, 1316, 2046
Bsu15I ATCGAT 1 cut(s) 1806
BsuI GTATCC 1 cut(s) 976
BsuRI GGCC 7 cut(s) 290, 348, 355, 521, 840, 1024, 2129
BsuTUI ATCGAT 1 cut(s) 1806
BtgI CCRYGG 2 cut(s) 70, 955
BtsCI GGATG 9 cut(s) 268, 499, 659, 716, 763, 1118, 1267, 1490, 1711
BtsIMutI CAGTG 1 cut(s) 1302
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 8 cut(s) 106, 123, 357, 563, 619, 1022, 1140, 1144
CaiI CAGNNNCTG 1 cut(s) 2215
CfoI GCGC 1 cut(s) 1584
Cfr10I RCCGGY 3 cut(s) 104, 368, 789
Cfr13I GGNCC 6 cut(s) 172, 371, 520, 581, 839, 1023
ClaI ATCGAT 1 cut(s) 1806
CseI GACGC 2 cut(s) 554, 1558
Csp6I GTAC 8 cut(s) 276, 540, 778, 880, 917, 1322, 1503, 2037
CspCI CAANNNNNGTGG 2 cut(s) 1653, 1688
CviQI GTAC 8 cut(s) 276, 540, 778, 880, 917, 1322, 1503, 2037
DdeI CTNAG 7 cut(s) 11, 146, 425, 464, 866, 1356, 1578
DpnI GATC 5 cut(s) 636, 1158, 1318, 1479, 2048
DpnII GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
DraI TTTAAA 1 cut(s) 597
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 4 cut(s) 111, 1014, 1056, 1398
EarI CTCTTC 4 cut(s) 111, 1014, 1056, 1398
Ecl136II GAGCTC 1 cut(s) 1661
Eco130I CCWWGG 3 cut(s) 70, 393, 955
Eco24I GRGCYC 2 cut(s) 1663, 2287
Eco31I GGTCTC 2 cut(s) 1366, 2130
Eco47I GGWCC 3 cut(s) 172, 371, 581
Eco47III AGCGCT 1 cut(s) 1583
Eco53kI GAGCTC 1 cut(s) 1661
Eco57I CTGAAG 2 cut(s) 135, 1310
Eco88I CYCGRG 1 cut(s) 1700
EcoICRI GAGCTC 1 cut(s) 1661
EcoNI CCTNNNNNAGG 1 cut(s) 2061
EcoO109I RGGNCCY 2 cut(s) 520, 581
EcoRI GAATTC 1 cut(s) 1340
EcoRII CCWGG 5 cut(s) 174, 662, 1251, 1643, 1783
EcoT14I CCWWGG 3 cut(s) 70, 393, 955
EcoT38I GRGCYC 2 cut(s) 1663, 2287
ErhI CCWWGG 3 cut(s) 70, 393, 955
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 2 cut(s) 1272, 1304
FaqI GGGAC 2 cut(s) 357, 2112
FauNDI CATATG 2 cut(s) 280, 1208
FbaI TGATCA 1 cut(s) 634
FblI GTMKAC 1 cut(s) 568
Fnu4HI GCNGC 8 cut(s) 102, 297, 312, 1649, 1827, 1932, 2160, 2256
FokI GGATG 9 cut(s) 275, 506, 646, 723, 750, 1125, 1274, 1497, 1718
FriOI GRGCYC 2 cut(s) 1663, 2287
Fsp4HI GCNGC 8 cut(s) 102, 297, 312, 1649, 1827, 1932, 2160, 2256
FspBI CTAG 2 cut(s) 525, 1266
GlaI GCGC 1 cut(s) 1583
GluI GCNGC 8 cut(s) 102, 297, 312, 1649, 1827, 1932, 2160, 2256
HaeII RGCGCY 1 cut(s) 1585
HaeIII GGCC 7 cut(s) 290, 348, 355, 521, 840, 1024, 2129
HapII CCGG 5 cut(s) 98, 105, 169, 369, 790
HgaI GACGC 2 cut(s) 554, 1558
HhaI GCGC 1 cut(s) 1584
Hin6I GCGC 1 cut(s) 1582
HinP1I GCGC 1 cut(s) 1582
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 3 cut(s) 8, 1281, 2165
HinfI GANTC 6 cut(s) 241, 628, 721, 1376, 1574, 1884
HpaII CCGG 5 cut(s) 98, 105, 169, 369, 790
HphI GGTGA 6 cut(s) 158, 278, 330, 838, 1430, 1574
Hpy166II GTNNAC 6 cut(s) 542, 569, 795, 1565, 1737, 2123
Hpy188III TCNNGA 5 cut(s) 632, 1055, 1771, 2060, 2317
Hpy8I GTNNAC 6 cut(s) 542, 569, 795, 1565, 1737, 2123
Hpy99I CGWCG 1 cut(s) 1552
HpyCH4III ACNGT 3 cut(s) 275, 997, 2072
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 365, 918, 1262
HpyF3I CTNAG 7 cut(s) 11, 146, 425, 464, 866, 1356, 1578
HspAI GCGC 1 cut(s) 1582
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
LguI GCTCTTC 2 cut(s) 111, 1056
LmnI GCTCC 2 cut(s) 1658, 1666
Lsp1109I GCAGC 8 cut(s) 88, 283, 323, 1660, 1838, 1918, 2146, 2267
LweI GCATC 8 cut(s) 430, 701, 772, 1125, 1192, 1252, 1533, 1582
MaeI CTAG 2 cut(s) 525, 1266
MaeIII GTNAC 6 cut(s) 164, 185, 1418, 1439, 1832, 2244
MalI GATC 5 cut(s) 636, 1158, 1318, 1479, 2048
MboI GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
MfeI CAATTG 3 cut(s) 1107, 1569, 2183
MflI RGATCY 3 cut(s) 1156, 1316, 2046
MhlI GDGCHC 4 cut(s) 619, 1663, 1739, 2287
MlyI GAGTC 1 cut(s) 1385
MmeI TCCRAC 3 cut(s) 1404, 1439, 1653
MroNI GCCGGC 1 cut(s) 104
MroXI GAANNNNTTC 4 cut(s) 138, 227, 1071, 2270
MseI TTAA 7 cut(s) 453, 596, 1130, 1176, 1371, 2111, 2175
MslI CAYNNNNRTG 5 cut(s) 560, 622, 642, 927, 1806
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 5 cut(s) 98, 105, 169, 369, 790
MspR9I CCNGG 7 cut(s) 98, 170, 176, 664, 1253, 1645, 1785
MssI GTTTAAAC 1 cut(s) 597
MunI CAATTG 3 cut(s) 1107, 1569, 2183
Mva1269I GAATGC 1 cut(s) 207
MvaI CCWGG 5 cut(s) 176, 664, 1253, 1645, 1785
MwoI GCNNNNNNNGC 4 cut(s) 296, 365, 918, 1262
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 2 cut(s) 98, 170
NcoI CCATGG 2 cut(s) 70, 955
NdeI CATATG 2 cut(s) 280, 1208
NdeII GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
NgoMIV GCCGGC 1 cut(s) 104
NlaIV GGNNCC 6 cut(s) 173, 373, 522, 583, 841, 1436
NmeAIII GCCGAG 2 cut(s) 419, 2155
NmuCI GTSAC 4 cut(s) 164, 185, 1418, 1439
NspI RCATGY 3 cut(s) 565, 1833, 1849
NspV TTCGAA 1 cut(s) 2075
OliI CACNNNNGTG 1 cut(s) 622
PaeI GCATGC 1 cut(s) 565
PaqCI CACCTGC 1 cut(s) 196
PasI CCCWGGG 1 cut(s) 1784
PciSI GCTCTTC 2 cut(s) 111, 1056
PcsI WCGNNNNNNNCGW 1 cut(s) 1707
PctI GAATGC 1 cut(s) 207
PdiI GCCGGC 1 cut(s) 106
PdmI GAANNNNTTC 4 cut(s) 138, 227, 1071, 2270
PfeI GAWTC 5 cut(s) 241, 628, 721, 1574, 1884
PkrI GCNGC 8 cut(s) 103, 298, 313, 1650, 1828, 1933, 2161, 2257
PleI GAGTC 1 cut(s) 1384
PmeI GTTTAAAC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 1384
PpuMI RGGWCCY 1 cut(s) 581
PshBI ATTAAT 3 cut(s) 1130, 2111, 2175
Psp124BI GAGCTC 1 cut(s) 1663
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 5 cut(s) 174, 662, 1251, 1643, 1783
PspGI CCWGG 5 cut(s) 174, 662, 1251, 1643, 1783
PspN4I GGNNCC 6 cut(s) 173, 373, 522, 583, 841, 1436
PspPI GGNCC 6 cut(s) 172, 371, 520, 581, 839, 1023
PspPPI RGGWCCY 1 cut(s) 581
PstI CTGCAG 1 cut(s) 1510
PstNI CAGNNNCTG 1 cut(s) 2215
PsuI RGATCY 3 cut(s) 1156, 1316, 2046
RsaI GTAC 8 cut(s) 277, 541, 779, 881, 918, 1323, 1504, 2038
RsaNI GTAC 8 cut(s) 276, 540, 778, 880, 917, 1322, 1503, 2037
RseI CAYNNNNRTG 5 cut(s) 560, 622, 642, 927, 1806
SacI GAGCTC 1 cut(s) 1663
SapI GCTCTTC 2 cut(s) 111, 1056
SaqAI TTAA 7 cut(s) 453, 596, 1130, 1176, 1371, 2111, 2175
SatI GCNGC 8 cut(s) 102, 297, 312, 1649, 1827, 1932, 2160, 2256
Sau3AI GATC 5 cut(s) 634, 1156, 1316, 1477, 2046
Sau96I GGNCC 6 cut(s) 172, 371, 520, 581, 839, 1023
SchI GAGTC 1 cut(s) 1385
ScrFI CCNGG 7 cut(s) 98, 170, 176, 664, 1253, 1645, 1785
SduI GDGCHC 4 cut(s) 619, 1663, 1739, 2287
SfaNI GCATC 8 cut(s) 430, 701, 772, 1125, 1192, 1252, 1533, 1582
SfcI CTRYAG 1 cut(s) 1506
SfuI TTCGAA 1 cut(s) 2075
SinI GGWCC 3 cut(s) 172, 371, 581
SmiMI CAYNNNNRTG 5 cut(s) 560, 622, 642, 927, 1806
SphI GCATGC 1 cut(s) 565
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 525, 1266
SstI GAGCTC 1 cut(s) 1663
StyD4I CCNGG 7 cut(s) 96, 168, 174, 662, 1251, 1643, 1783
StyI CCWWGG 3 cut(s) 70, 393, 955
TaaI ACNGT 3 cut(s) 275, 997, 2072
TaqI TCGA 5 cut(s) 93, 199, 1096, 1806, 2075
TaqII GACCGA 1 cut(s) 1488
TatI WGTACW 7 cut(s) 275, 539, 777, 879, 1321, 1502, 2036
TfiI GAWTC 5 cut(s) 241, 628, 721, 1574, 1884
Tru1I TTAA 7 cut(s) 453, 596, 1130, 1176, 1371, 2111, 2175
Tru9I TTAA 7 cut(s) 453, 596, 1130, 1176, 1371, 2111, 2175
TscAI CASTG 1 cut(s) 1309
TseFI GTSAC 4 cut(s) 164, 185, 1418, 1439
TseI GCWGC 8 cut(s) 101, 296, 311, 1648, 1826, 1931, 2159, 2255
Tsp45I GTSAC 4 cut(s) 164, 185, 1418, 1439
TspGWI ACGGA 1 cut(s) 1427
TspRI CASTG 1 cut(s) 1309
VneI GTGCAC 1 cut(s) 1735
VpaK11BI GGWCC 3 cut(s) 172, 371, 581
VspI ATTAAT 3 cut(s) 1130, 2111, 2175
XagI CCTNNNNNAGG 1 cut(s) 2061
XapI RAATTY 5 cut(s) 900, 1340, 1694, 1979, 2077
XceI RCATGY 3 cut(s) 565, 1833, 1849
XmiI GTMKAC 1 cut(s) 568
XmnI GAANNNNTTC 4 cut(s) 138, 227, 1071, 2270
XspI CTAG 2 cut(s) 525, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.