RchiOBHm_Chr2g0148941

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000173
Physical Location & Seq
Forward (+)
66756614 .. 66761534
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51838

Sequence Viewer

Length: 2340 bp
ATGTGGAAGCTTAAGTTGCAGAGGTGGGATCTCGGCTTACATTACAGCACAACAACTTTGTCGGAAGGCAGACATGGGAGTTTGATTCGGATGCAGGAACTCCTGAAGCGAGCTGAGGTTGAAGAGGCCCGTCTTCATTTCTACAACAACCGCTATCAGGTCAAGCCCAGTGGTGACATTCTTTGGCGTATGCAGTTCCTAAGGGAGAAGAAGTTCAAACAGACAATTACTCCAGTAAAAATTGAAGATGGCGAGGTTATCACACATGAAAAAACCACAGATGCACTGAGAAGGTCTGTCCACTTCTTTTCAGCGTTGCAGGCAAGCGATGGCCATTGGCCTGCGGAAAATGCCGGCCCTTTGTTTTTCCTTCCTCCCTTGGTCATATGTATGTACATTACTGGCCATCTTAACAGTGTATTCCCTGAAGAGCATCGCAAAGAAATTCTGCGTTACATATACTATCATCAGAATGAAGATGGTGGTTGGGGACTACACGTCGAAGGTCACAGCACCATGTTCAGCACAGCTCTCAGCTACATTTGTATGCGTATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAATGCTTGTCCAAGAGCAAGAAAGTGGATTCTTGATCACGGTGGTGTCACACACATACCCTCTTGGGGAAAGACTTGGCTTTCGCAACCCTATGCCCCAGAGTTTTGGATTCTTCCTTCGTTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTATTGTAGGATGGTTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCCCAATTACACCTCTCATTCTTAAATTGAGGGAGGAACTTTTTATTCAACCTTACAATCAAATCAATTGGAAGAAAGTACGCCATCTATGTGCCAAAGAGGACATCTACTATCCTCATCCTTGGATACAGGATCTCCTATGGGATAGCCTCTACATTTCCACAGAGCCTATTCTTACTCGTTGGCCCTTCAACAAATTGATCAGAGAAAAGGCTCTTCAAGTGACGATGAAGCATATTCATTACGAAGATGAGAACAGTCGATACATTACCATTGGGTGTGTGGAAAAGGTGTTATGTATGCTTGCCTGTTGGGTTGAAGATCCAAATGGGGACTATTTCAAGAAGCATCTGGCAAGGGTCCCGGATTATTTATGGGTTGCTGAAGATGGAATGAAGATGCAAAGTTTTGGCAGTCAGGAGTGGGATACTGGTTTTGCCATTCAAGCTTTGCTTGCTACTAATCTAACGGACGAAATTGGAACAACGCTCGCTAGAGGACATGACTTCATAAAGAAATCTCAGGTCAAAGACAACCCATCTGGTGACTTCAATAGCATGCACCGCCACATTTCCAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAAGTTTCTGATTGCACTGCAGAAGGTTTAAAGTGTTGCCTTCTATTGTCGATGATGTCATCGGAGATAGTTGGTGAAAAATGGAACCTGAGCGCTTGTACGATTCTGTCAATGTTCTACTTTCCCTACAGCAAGAATGGTGGTTTGTCAGCCTGGGAACCAGCAGGGGCAGCAGAGTGGTTAGAACTATTAAATCCGACAGAGTTCTTCGCAGACATAGTAATTGAGCATGAATATGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTACTGTTTAAGAAGCTACACCCAGGACACCGGAAGAAAGAGATTGAACATTTCATCACCAATGCTATACACTACCTTGAAAATATACAAATGCCGGATGGTTCATGGTATGTATCTATTCTAGCAAATCAATCTATGATTCGAAAACGTGTTAGTTTCACATATACAGAAAAAAAGAACTTGATAAGTAAGCATTTGAACAGGTATGGAAACTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCAGTAGGAGGATTGGCAGCAGCTGGCAAGACTTTCAGCACTTGTGCAGCCATGCGCAAAGCGGTTAGTTTTCTCCTCACAACGCAGAGAGAGAATGGTGGTTGGGGAGAGAGCTATCTTTCATGTCCGCAAGAGACATACATTCCTCTCGAAGGAAATCGATCCAATTTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCAACACCTCTTCATCGTGCAGCAAAGTTTATAATTAATTCTCAAATGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCATGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATATACCCACTCTGGGCTCTTGCAGAATACCGCAAGCGGGTTCCATTGCCTTCCTAG

Protein Analysis

779

Amino Acids

90.01

Weight (kDa)

7.6

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 97 - 347 1.6e-23 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 406 - 597 1.6e-09 Squalene-hopene cyclase C-terminal domain
SQHop_cyclase_C PF13243 625 - 773 7.8e-30 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2186
Acc16I TGCGCA 1 cut(s) 1982
Acc65I GGTACC 1 cut(s) 1916
AccB1I GGYRCC 1 cut(s) 1916
AciI CCGC 8 cut(s) 151, 344, 1360, 1988, 2054, 2135, 2314, 2320
AclWI GGATC 5 cut(s) 36, 936, 1112, 1384, 2082
AcoI YGGCCR 3 cut(s) 331, 403, 580
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 3 cut(s) 125, 447, 1200
AfaI GTAC 6 cut(s) 395, 876, 1510, 1683, 1918, 2100
AfeI AGCGCT 1 cut(s) 1504
AfiI CCNNNNNNNGG 6 cut(s) 157, 575, 653, 2078, 2296, 2320
AflII CTTAAG 1 cut(s) 11
AflIII ACRYGT 2 cut(s) 496, 1828
AhdI GACNNNNNGTC 1 cut(s) 497
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 4 cut(s) 1562, 1671, 1702, 1919
AleI CACNNNNGTG 1 cut(s) 630
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 9 cut(s) 10, 113, 530, 537, 1244, 1696, 1949, 2040, 2273
AluI AGCT 9 cut(s) 10, 113, 530, 537, 1244, 1696, 1949, 2040, 2273
Alw21I GWGCWC 1 cut(s) 1658
Alw26I GTCTC 3 cut(s) 2054, 2129, 2145
Alw44I GTGCAC 1 cut(s) 1654
AlwI GGATC 5 cut(s) 36, 936, 1112, 1384, 2082
AlwNI CAGNNNCTG 1 cut(s) 1949
Aor51HI AGCGCT 1 cut(s) 1504
AoxI GGCC 8 cut(s) 126, 331, 338, 355, 403, 580, 796, 980
ApaLI GTGCAC 1 cut(s) 1654
ApeKI GCWGC 6 cut(s) 1580, 1943, 1946, 1973, 2174, 2270
ApoI RAATTY 1 cut(s) 444
ArsI GACNNNNNNTTYG 2 cut(s) 652, 684
AseI ATTAAT 1 cut(s) 2190
Asp700I GAANNNNTTC 1 cut(s) 212
Asp718I GGTACC 1 cut(s) 1916
AspLEI GCGC 2 cut(s) 1505, 1983
AspS9I GGNCC 6 cut(s) 127, 356, 566, 797, 981, 1156
AsuC2I CCSGG 1 cut(s) 1160
AsuHPI GGTGA 5 cut(s) 185, 1352, 1496, 1729, 2222
AsuII TTCGAA 1 cut(s) 1822
AvaII GGWCC 2 cut(s) 566, 1156
AxyI CCTNAGG 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 1658
BalI TGGCCA 3 cut(s) 333, 405, 582
BanI GGYRCC 1 cut(s) 1916
BanII GRGCYC 1 cut(s) 2302
BbsI GAAGAC 2 cut(s) 125, 2233
Bbv12I GWGCWC 1 cut(s) 1658
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 6 cut(s) 1592, 1955, 1958, 1985, 2186, 2282
BciT130I CCWGG 4 cut(s) 1564, 1673, 1704, 1921
BciVI GTATCC 2 cut(s) 915, 1216
BclI TGATCA 2 cut(s) 622, 996
BcnI CCSGG 1 cut(s) 1160
BcoDI GTCTC 3 cut(s) 2054, 2129, 2145
BfaI CTAG 3 cut(s) 1290, 1802, 2338
BfmI CTRYAG 2 cut(s) 1428, 1537
BfoI RGCGCY 1 cut(s) 1506
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 2 cut(s) 915, 1216
BisI GCNGC 6 cut(s) 1581, 1944, 1947, 1974, 2175, 2271
BlsI GCNGC 6 cut(s) 1582, 1945, 1948, 1975, 2176, 2272
BmcAI AGTACT 1 cut(s) 1683
Bme1390I CCNGG 5 cut(s) 1160, 1564, 1673, 1704, 1921
Bme18I GGWCC 2 cut(s) 566, 1156
BmeRI GACNNNNNGTC 1 cut(s) 497
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 6 cut(s) 127, 356, 566, 797, 981, 1156
BmiI GGNNCC 6 cut(s) 1157, 1158, 1496, 1569, 1918, 2325
BmrFI CCNGG 5 cut(s) 1160, 1564, 1673, 1704, 1921
BmrI ACTGGG 2 cut(s) 162, 1903
BmsI GCATC 6 cut(s) 81, 271, 442, 730, 1153, 1185
BmuI ACTGGG 2 cut(s) 162, 1903
BpiI GAAGAC 2 cut(s) 125, 2233
BpmI CTGGAG 2 cut(s) 216, 2256
Bpu10I CCTNAGC 2 cut(s) 114, 1499
Bpu14I TTCGAA 1 cut(s) 1822
BpuMI CCSGG 1 cut(s) 1160
Bsa29I ATCGAT 1 cut(s) 2086
BsaBI GATNNNNATC 1 cut(s) 1398
BsaI GGTCTC 2 cut(s) 2129, 2145
BsaJI CCNNGG 4 cut(s) 378, 917, 1563, 1702
BsaWI WCCGGW 1 cut(s) 1710
BsaXI ACNNNNNCTCC 6 cut(s) 197, 214, 227, 244, 915, 945
Bsc4I CCNNNNNNNGG 6 cut(s) 157, 575, 653, 2078, 2296, 2320
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 6 cut(s) 168, 233, 406, 1231, 1898, 2239
Bse21I CCTNAGG 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 2327
Bse8I GATNNNNATC 1 cut(s) 1398
BseBI CCWGG 4 cut(s) 1564, 1673, 1704, 1921
BseCI ATCGAT 1 cut(s) 2086
BseDI CCNNGG 4 cut(s) 378, 917, 1563, 1702
BseGI GGATG 6 cut(s) 96, 721, 756, 913, 1412, 1783
BseJI GATNNNNATC 1 cut(s) 1398
BseLI CCNNNNNNNGG 6 cut(s) 157, 575, 653, 2078, 2296, 2320
BseMI GCAATG 1 cut(s) 2327
BseMII CTCAG 5 cut(s) 105, 278, 547, 1331, 1490
BseNI ACTGG 6 cut(s) 168, 233, 406, 1231, 1898, 2239
BseRI GAGGAG 1 cut(s) 1991
BseSI GKGCMC 1 cut(s) 1658
BseXI GCAGC 6 cut(s) 1592, 1955, 1958, 1985, 2186, 2282
BseYI CCCAGC 1 cut(s) 2220
BsgI GTGCAG 2 cut(s) 1992, 2193
BshFI GGCC 8 cut(s) 128, 333, 340, 357, 405, 582, 798, 982
BshNI GGYRCC 1 cut(s) 1916
BshVI ATCGAT 1 cut(s) 2086
BsiHKAI GWGCWC 1 cut(s) 1658
BsiSI CCGG 4 cut(s) 354, 1160, 1711, 1775
BslFI GGGAC 4 cut(s) 504, 1142, 1142, 2151
BslI CCNNNNNNNGG 6 cut(s) 157, 575, 653, 2078, 2296, 2320
BsmAI GTCTC 3 cut(s) 2054, 2129, 2145
BsmFI GGGAC 4 cut(s) 504, 1142, 1142, 2151
BsnI GGCC 8 cut(s) 128, 333, 340, 357, 405, 582, 798, 982
Bso31I GGTCTC 2 cut(s) 2129, 2145
Bsp119I TTCGAA 1 cut(s) 1822
Bsp1286I GDGCHC 2 cut(s) 1658, 2302
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
BspACI CCGC 8 cut(s) 151, 344, 1360, 1988, 2054, 2135, 2314, 2320
BspANI GGCC 8 cut(s) 128, 333, 340, 357, 405, 582, 798, 982
BspCNI CTCAG 5 cut(s) 106, 279, 546, 1330, 1491
BspDI ATCGAT 1 cut(s) 2086
BspHI TCATGA 1 cut(s) 2244
BspLI GGNNCC 6 cut(s) 1157, 1158, 1496, 1569, 1918, 2325
BspMAI CTGCAG 1 cut(s) 1432
BspPI GGATC 5 cut(s) 36, 936, 1112, 1384, 2082
BspQI GCTCTTC 2 cut(s) 423, 1017
BspT104I TTCGAA 1 cut(s) 1822
BspT107I GGYRCC 1 cut(s) 1916
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 2129, 2145
BsrDI GCAATG 1 cut(s) 2327
BsrFI RCCGGY 1 cut(s) 353
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 6 cut(s) 168, 233, 406, 1231, 1898, 2239
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 4 cut(s) 378, 917, 1563, 1702
BssMI GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
BssT1I CCWWGG 2 cut(s) 378, 917
Bst2UI CCWGG 4 cut(s) 1564, 1673, 1704, 1921
Bst4CI ACNGT 4 cut(s) 416, 629, 1055, 1686
Bst6I CTCTTC 4 cut(s) 117, 423, 1017, 2169
BstAFI CTTAAG 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 1822
BstDEI CTNAG 6 cut(s) 114, 200, 287, 533, 1317, 1499
BstENI CCTNNNNNAGG 1 cut(s) 2076
BstF5I GGATG 6 cut(s) 96, 721, 756, 913, 1412, 1783
BstH2I RGCGCY 1 cut(s) 1506
BstHHI GCGC 2 cut(s) 1505, 1983
BstKTI GATC 9 cut(s) 31, 625, 931, 999, 1120, 1379, 1396, 1402, 2090
BstMAI GTCTC 3 cut(s) 2054, 2129, 2145
BstMBI GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
BstMWI GCNNNNNNNGC 9 cut(s) 16, 320, 350, 1241, 1250, 1359, 1580, 1673, 2141
BstNI CCWGG 4 cut(s) 1564, 1673, 1704, 1921
BstNSI RCATGY 3 cut(s) 763, 1357, 2260
BstSCI CCNGG 5 cut(s) 1158, 1562, 1671, 1702, 1919
BstSFI CTRYAG 2 cut(s) 1428, 1537
BstSLI GKGCMC 1 cut(s) 1658
BstV1I GCAGC 6 cut(s) 1592, 1955, 1958, 1985, 2186, 2282
BstV2I GAAGAC 2 cut(s) 125, 2233
BstX2I RGATCY 3 cut(s) 28, 928, 1117
BstXI CCANNNNNNTGG 1 cut(s) 693
BstYI RGATCY 3 cut(s) 28, 928, 1117
Bsu15I ATCGAT 1 cut(s) 2086
Bsu36I CCTNAGG 1 cut(s) 200
BsuI GTATCC 2 cut(s) 915, 1216
BsuRI GGCC 8 cut(s) 128, 333, 340, 357, 405, 582, 798, 982
BsuTUI ATCGAT 1 cut(s) 2086
BtgZI GCGATG 2 cut(s) 342, 419
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 6 cut(s) 96, 721, 756, 913, 1412, 1783
BtsI GCAGTG 1 cut(s) 1425
BtsIMutI CAGTG 5 cut(s) 175, 284, 421, 1425, 2232
CaiI CAGNNNCTG 1 cut(s) 1949
CciI TCATGA 1 cut(s) 2244
CfoI GCGC 2 cut(s) 1505, 1983
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 6 cut(s) 127, 356, 566, 797, 981, 1156
ClaI ATCGAT 1 cut(s) 2086
CsiI ACCWGGT 1 cut(s) 1919
Csp6I GTAC 6 cut(s) 394, 875, 1509, 1682, 1917, 2099
CspCI CAANNNNNGTGG 4 cut(s) 151, 186, 1531, 1566
CviQI GTAC 6 cut(s) 394, 875, 1509, 1682, 1917, 2099
DdeI CTNAG 6 cut(s) 114, 200, 287, 533, 1317, 1499
DpnI GATC 9 cut(s) 30, 624, 930, 998, 1119, 1378, 1395, 1401, 2089
DpnII GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
DraI TTTAAA 1 cut(s) 1440
DriI GACNNNNNGTC 1 cut(s) 497
EaeI YGGCCR 3 cut(s) 331, 403, 580
Eam1104I CTCTTC 4 cut(s) 117, 423, 1017, 2169
Eam1105I GACNNNNNGTC 1 cut(s) 497
EarI CTCTTC 4 cut(s) 117, 423, 1017, 2169
Eco130I CCWWGG 2 cut(s) 378, 917
Eco24I GRGCYC 1 cut(s) 2302
Eco31I GGTCTC 2 cut(s) 2129, 2145
Eco47I GGWCC 2 cut(s) 566, 1156
Eco47III AGCGCT 1 cut(s) 1504
Eco57I CTGAAG 3 cut(s) 125, 447, 1200
Eco81I CCTNAGG 1 cut(s) 200
EcoNI CCTNNNNNAGG 1 cut(s) 2076
EcoO109I RGGNCCY 2 cut(s) 566, 1156
EcoRII CCWGG 4 cut(s) 1562, 1671, 1702, 1919
EcoT14I CCWWGG 2 cut(s) 378, 917
EcoT22I ATGCAT 1 cut(s) 723
EcoT38I GRGCYC 1 cut(s) 2302
ErhI CCWWGG 2 cut(s) 378, 917
FalI AAGNNNNNCTT 2 cut(s) 1233, 1265
FaqI GGGAC 4 cut(s) 504, 1142, 1142, 2151
FauI CCCGC 2 cut(s) 2128, 2313
FauNDI CATATG 2 cut(s) 386, 1912
FbaI TGATCA 2 cut(s) 622, 996
Fnu4HI GCNGC 6 cut(s) 1581, 1944, 1947, 1974, 2175, 2271
FokI GGATG 6 cut(s) 103, 708, 763, 900, 1419, 1790
FriOI GRGCYC 1 cut(s) 2302
Fsp4HI GCNGC 6 cut(s) 1581, 1944, 1947, 1974, 2175, 2271
FspBI CTAG 3 cut(s) 1290, 1802, 2338
FspI TGCGCA 1 cut(s) 1982
GlaI GCGC 2 cut(s) 1504, 1982
GluI GCNGC 6 cut(s) 1581, 1944, 1947, 1974, 2175, 2271
GsaI CCCAGC 1 cut(s) 2224
GsuI CTGGAG 2 cut(s) 216, 2256
HaeII RGCGCY 1 cut(s) 1506
HaeIII GGCC 8 cut(s) 128, 333, 340, 357, 405, 582, 798, 982
HapII CCGG 4 cut(s) 354, 1160, 1711, 1775
HhaI GCGC 2 cut(s) 1505, 1983
Hin6I GCGC 2 cut(s) 1503, 1981
HinP1I GCGC 2 cut(s) 1503, 1981
HindIII AAGCTT 2 cut(s) 8, 1242
HinfI GANTC 6 cut(s) 85, 616, 697, 1513, 1819, 2238
HpaII CCGG 4 cut(s) 354, 1160, 1711, 1775
HphI GGTGA 5 cut(s) 185, 1352, 1496, 1729, 2222
Hpy166II GTNNAC 4 cut(s) 301, 757, 1383, 1656
Hpy188I TCNGA 9 cut(s) 64, 90, 471, 560, 1001, 1393, 1420, 1474, 1608
Hpy188III TCNNGA 7 cut(s) 103, 620, 1138, 1214, 1397, 2075, 2245
Hpy8I GTNNAC 4 cut(s) 301, 757, 1383, 1656
Hpy99I CGWCG 1 cut(s) 503
HpyCH4III ACNGT 4 cut(s) 416, 629, 1055, 1686
HpyCH4IV ACGT 2 cut(s) 498, 1828
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 320, 350, 1241, 1250, 1359, 1580, 1673, 2141
HpyF3I CTNAG 6 cut(s) 114, 200, 287, 533, 1317, 1499
HpySE526I ACGT 2 cut(s) 498, 1828
HspAI GCGC 2 cut(s) 1503, 1981
KflI GGGWCCC 1 cut(s) 1156
KpnI GGTACC 1 cut(s) 1920
KroI GCCGGC 1 cut(s) 353
KroNI GCCGGC 1 cut(s) 355
Ksp22I TGATCA 2 cut(s) 622, 996
Kzo9I GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
LguI GCTCTTC 2 cut(s) 423, 1017
Lsp1109I GCAGC 6 cut(s) 1592, 1955, 1958, 1985, 2186, 2282
LweI GCATC 6 cut(s) 81, 271, 442, 730, 1153, 1185
MabI ACCWGGT 1 cut(s) 1919
MaeI CTAG 3 cut(s) 1290, 1802, 2338
MaeII ACGT 2 cut(s) 498, 1828
MaeIII GTNAC 6 cut(s) 173, 452, 506, 634, 1018, 1340
MalI GATC 9 cut(s) 30, 624, 930, 998, 1119, 1378, 1395, 1401, 2089
MboI GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
MfeI CAATTG 1 cut(s) 862
MflI RGATCY 3 cut(s) 28, 928, 1117
MhlI GDGCHC 2 cut(s) 1658, 2302
MlsI TGGCCA 3 cut(s) 333, 405, 582
MluNI TGGCCA 3 cut(s) 333, 405, 582
MlyI GAGTC 1 cut(s) 2247
MmeI TCCRAC 2 cut(s) 42, 1631
Mox20I TGGCCA 3 cut(s) 333, 405, 582
Mph1103I ATGCAT 1 cut(s) 723
MroNI GCCGGC 1 cut(s) 353
MroXI GAANNNNTTC 1 cut(s) 212
MscI TGGCCA 3 cut(s) 333, 405, 582
MseI TTAA 7 cut(s) 12, 411, 819, 1439, 1601, 1689, 2190
MslI CAYNNNNRTG 7 cut(s) 389, 471, 545, 630, 764, 885, 1644
Msp20I TGGCCA 3 cut(s) 333, 405, 582
MspA1I CMGCKG 1 cut(s) 1949
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 4 cut(s) 354, 1160, 1711, 1775
MspR9I CCNGG 5 cut(s) 1160, 1564, 1673, 1704, 1921
MunI CAATTG 1 cut(s) 862
MvaI CCWGG 4 cut(s) 1564, 1673, 1704, 1921
MwoI GCNNNNNNNGC 9 cut(s) 16, 320, 350, 1241, 1250, 1359, 1580, 1673, 2141
NaeI GCCGGC 1 cut(s) 355
NciI CCSGG 1 cut(s) 1160
NdeI CATATG 2 cut(s) 386, 1912
NdeII GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
NgoMIV GCCGGC 1 cut(s) 353
NlaIV GGNNCC 6 cut(s) 1157, 1158, 1496, 1569, 1918, 2325
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 5 cut(s) 173, 506, 634, 1018, 1340
NsbI TGCGCA 1 cut(s) 1982
NsiI ATGCAT 1 cut(s) 723
NspI RCATGY 3 cut(s) 763, 1357, 2260
NspV TTCGAA 1 cut(s) 1822
OliI CACNNNNGTG 1 cut(s) 630
PaeI GCATGC 2 cut(s) 1357, 2260
PagI TCATGA 1 cut(s) 2244
PciSI GCTCTTC 2 cut(s) 423, 1017
PdiI GCCGGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 5 cut(s) 85, 616, 697, 1513, 1819
PfoI TCCNGGA 1 cut(s) 1158
PkrI GCNGC 6 cut(s) 1582, 1945, 1948, 1975, 2176, 2272
PleI GAGTC 1 cut(s) 2246
PpsI GAGTC 1 cut(s) 2246
PpuMI RGGWCCY 2 cut(s) 566, 1156
PshBI ATTAAT 1 cut(s) 2190
PsiI TTATAA 1 cut(s) 2186
Psp5II RGGWCCY 2 cut(s) 566, 1156
Psp6I CCWGG 4 cut(s) 1562, 1671, 1702, 1919
PspFI CCCAGC 1 cut(s) 2220
PspGI CCWGG 4 cut(s) 1562, 1671, 1702, 1919
PspN4I GGNNCC 6 cut(s) 1157, 1158, 1496, 1569, 1918, 2325
PspPI GGNCC 6 cut(s) 127, 356, 566, 797, 981, 1156
PspPPI RGGWCCY 2 cut(s) 566, 1156
PsrI GAACNNNNNNTAC 2 cut(s) 1043, 1075
PstI CTGCAG 1 cut(s) 1432
PstNI CAGNNNCTG 1 cut(s) 1949
PsuI RGATCY 3 cut(s) 28, 928, 1117
PvuII CAGCTG 1 cut(s) 1949
RsaI GTAC 6 cut(s) 395, 876, 1510, 1683, 1918, 2100
RsaNI GTAC 6 cut(s) 394, 875, 1509, 1682, 1917, 2099
RseI CAYNNNNRTG 7 cut(s) 389, 471, 545, 630, 764, 885, 1644
SapI GCTCTTC 2 cut(s) 423, 1017
SaqAI TTAA 7 cut(s) 12, 411, 819, 1439, 1601, 1689, 2190
SatI GCNGC 6 cut(s) 1581, 1944, 1947, 1974, 2175, 2271
Sau3AI GATC 9 cut(s) 28, 622, 928, 996, 1117, 1376, 1393, 1399, 2087
Sau96I GGNCC 6 cut(s) 127, 356, 566, 797, 981, 1156
ScaI AGTACT 1 cut(s) 1683
SchI GAGTC 1 cut(s) 2247
ScrFI CCNGG 5 cut(s) 1160, 1564, 1673, 1704, 1921
SduI GDGCHC 2 cut(s) 1658, 2302
SexAI ACCWGGT 1 cut(s) 1919
SfaNI GCATC 6 cut(s) 81, 271, 442, 730, 1153, 1185
SfcI CTRYAG 2 cut(s) 1428, 1537
SfuI TTCGAA 1 cut(s) 1822
SinI GGWCC 2 cut(s) 566, 1156
SmiMI CAYNNNNRTG 7 cut(s) 389, 471, 545, 630, 764, 885, 1644
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SphI GCATGC 2 cut(s) 1357, 2260
SsiI CCGC 8 cut(s) 151, 344, 1360, 1988, 2054, 2135, 2314, 2320
SspMI CTAG 3 cut(s) 1290, 1802, 2338
StyD4I CCNGG 5 cut(s) 1158, 1562, 1671, 1702, 1919
StyI CCWWGG 2 cut(s) 378, 917
TaaI ACNGT 4 cut(s) 416, 629, 1055, 1686
TaiI ACGT 2 cut(s) 501, 1831
TaqI TCGA 6 cut(s) 501, 1057, 1460, 1822, 2076, 2086
TatI WGTACW 3 cut(s) 393, 1681, 2098
TfiI GAWTC 5 cut(s) 85, 616, 697, 1513, 1819
Tru1I TTAA 7 cut(s) 12, 411, 819, 1439, 1601, 1689, 2190
Tru9I TTAA 7 cut(s) 12, 411, 819, 1439, 1601, 1689, 2190
TscAI CASTG 5 cut(s) 175, 291, 421, 1432, 2239
TseFI GTSAC 5 cut(s) 173, 506, 634, 1018, 1340
TseI GCWGC 6 cut(s) 1580, 1943, 1946, 1973, 2174, 2270
Tsp45I GTSAC 5 cut(s) 173, 506, 634, 1018, 1340
TspGWI ACGGA 1 cut(s) 1280
TspRI CASTG 5 cut(s) 175, 291, 421, 1432, 2239
Vha464I CTTAAG 1 cut(s) 11
VneI GTGCAC 1 cut(s) 1654
VpaK11BI GGWCC 2 cut(s) 566, 1156
VspI ATTAAT 1 cut(s) 2190
XagI CCTNNNNNAGG 1 cut(s) 2076
XapI RAATTY 1 cut(s) 444
XceI RCATGY 3 cut(s) 763, 1357, 2260
XcmI CCANNNNNNNNNTGG 2 cut(s) 732, 1075
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 3 cut(s) 1290, 1802, 2338
ZrmI AGTACT 1 cut(s) 1683
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.