RchiOBHm_Chr2g0148451

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000173
Physical Location & Seq
Forward (+)
66182058 .. 66188637
6580 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51802

Sequence Viewer

Length: 2085 bp
ATGTGGAAGCTTAGGTTTGGAGACGAAGGTGCAAATAATGACCCTTACTTGTTCAGCACAAACAACTTCCATGGGAGGCAAACATGGGAGTTCGACCCGGCTGCCGGCACACCTGAAGAGCGAGCAGAGGTAGAAGAAGTTCGGCTAAGTTTTTACAACAATCGTCACCGAGTCCAGGCTTGTAGTGACCTCCTCTGTCGAATGCAGCTTCTGGGAGAGAAAAAGTTCAAACAAAAGATTCCCCAAGTCAAGGTGGAGGATGGTGAGGACTGTACATATGAAAAGGCCACCGCTGCATTTAGAAGGGCAGCTACCTTCTACTCTGCCTTACAATCACCTCACGGCCATTGGCCTGCTGAAAATGCCGGTCCCATGTTTTATATTCCTCCCTTGGTCATATCGCTCTATATTACGGGGCATCTTAGTAAGGTATTCTCTGCAGAGCATTTTAAGGAAATGCTTAGATATATATACTGTCATCAGAATGAGGATGGTGGGTGGGGATTACACATACAGGCCCCTAGTATGATGTTTTGTACACTTCTGAATTACATTTGCATGCGTCTACTTGGAGAAGGACCCGATGGTGGTTTAAACAACGCTTGTGCAAGAGCACGCAAGTGGATTCTTGATCATGGTGGTGCAACACATTCATCATCCTGGGGAAAGACTTATATGGCTATACTCGGTGTGTATGAGTGGGAGGGATGCAACCCGATTCCCCCAGAGTTTTGGATTTTCCCGTCTTTGTTCCCTTTGCATCCAGCAAAAATGTACTGCTACTGCCGGTTGACTTACTTGCCTATATCGTATCTGTTTGGGAAAAGATTTGTTGGCCCCATCACCCCTCTCATTCTACAACTGAGAGAAGAAATGTACAATGAGCCATACAATGAAATTCAATGGGGCAAAGTACGCCATCTCTGTGCAAAGCCGTTAATGATGCTTGCTTGCTGGATTGAAGATCCGAATGGAAAACCCTTTAAGAAGCATCTTGCAAGAGTTATAGATTACATATGGGTTGCAGAAGATGGAATAACTACGCAGGCATGGGATGCTAGTCTTTCTGTTCAAGCTTTGCTTGCTGGAAATCACACTGATGAACTTGGATCTGTACTTCAGAAAGCACATGAATTCCTAAACAAATCTCAGATTAGGGATAATCCTTCTGGTGACTTCTTATCTCGGTTCCGTCACATTTCTAAAGGTGGTTGGACTTTCTCTGACCGAGATCATGGATGGCAAGTGTCTGATTGTACTGCAGAGTGTTTGAAGTGTTGCCTATTGCTTTCAATGATGCCGACGCAAATCGTGGGTGAACCAGTTGAATATGAGCGCCTGTATGATGCTGTCAATGTCATACTTTCTCTACAAAATCCAAATGGTGGTGTATCAGCCTGGCAGCCAACAGGAGCTCCACAATGGTTGGAGTGGTTCAATCCAGTAGAATTTCTCGAGGATGTCGTCATTGAATACGAGTATGTTGAGTGCACTTCATCTTCAATTCAAGCGTTAGCTTTATTCAGGAAGTTATACCCTGGGCATAGAAAGAAACATATCGATGATTTCATCACAAATGCAGCATGTTACCTTGAAGACATGCAGACACCTGATGGCTCATGGTATGGAAGGTGGGGAATCTGCTTCATCTATGGAACATGGTTTGCAATCCGAGGGTTGAAGGCTGCTGGTAGAACTTACAAAAATTGTGAGGCGATACGCAGAGGTGTTGAATTTATACTCAAAATACAGAGAAATGATGGTGGTTGGGGAGAGAGCTATACTTCTTGTACAAACAAGATCTATACACCTCTTCAAGGCGACCGGTCGAATTTGGTGCAGACTGCAATGGGACTAATGGGATTAATTCATGGGGGACAGGCCGAGAGAGACCCTACTCCCATTCACAGAGCTGCGAAGCTTTTGATTAATTCTCAATTGGAAAATGGAGATTTTCCCCAACAGGAACTGATGGGGGTTTTCAATGTCAATGCCATGTTACACTATGCAGCATACAGGAATATCTTCCCAATTTGGGCTCTCGGAGAATATTGTTCCCTTCCCTCCTTCTGGATAGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

694

Amino Acids

79.27

Weight (kDa)

6.14

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 102 - 302 8.6e-34 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 350 - 684 5.5e-48 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccI GTMKAC 1 cut(s) 565
AciI CCGC 1 cut(s) 291
AclWI GGATC 2 cut(s) 959, 1117
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 5 cut(s) 897, 1133, 1448, 1733, 1831
AcuI CTGAAG 2 cut(s) 135, 1103
AfaI GTAC 8 cut(s) 274, 538, 776, 878, 915, 1116, 1258, 1792
AfiI CCNNNNNNNGG 8 cut(s) 104, 175, 250, 587, 1385, 1817, 2035, 2070
AgeI ACCGGT 1 cut(s) 1824
AjnI CCWGG 4 cut(s) 174, 659, 1397, 1537
AleI CACNNNNGTG 1 cut(s) 619
AluBI AGCT 9 cut(s) 10, 208, 311, 1076, 1415, 1517, 1779, 1913, 1921
AluI AGCT 9 cut(s) 10, 208, 311, 1076, 1415, 1517, 1779, 1913, 1921
Alw21I GWGCWC 3 cut(s) 616, 1417, 1493
Alw26I GTCTC 2 cut(s) 15, 1884
Alw44I GTGCAC 1 cut(s) 1489
AlwI GGATC 2 cut(s) 959, 1117
AlwNI CAGNNNCTG 2 cut(s) 211, 1969
Ama87I CYCGRG 1 cut(s) 1454
AoxI GGCC 6 cut(s) 285, 343, 350, 516, 835, 1881
ApaLI GTGCAC 1 cut(s) 1489
ApeKI GCWGC 9 cut(s) 101, 205, 293, 308, 1402, 1580, 1685, 1913, 2009
ApoI RAATTY 5 cut(s) 897, 1133, 1448, 1733, 1831
AseI ATTAAT 2 cut(s) 1865, 1929
AsiGI ACCGGT 1 cut(s) 1824
Asp700I GAANNNNTTC 3 cut(s) 138, 224, 2024
AspLEI GCGC 1 cut(s) 1338
AspS9I GGNCC 4 cut(s) 368, 517, 578, 836
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 6 cut(s) 158, 275, 327, 835, 1184, 1328
AvaI CYCGRG 1 cut(s) 1454
AvaII GGWCC 2 cut(s) 368, 578
BaeGI GKGCMC 1 cut(s) 1493
BanII GRGCYC 2 cut(s) 1417, 2041
BarI GAAGNNNNNNTAC 2 cut(s) 295, 327
BbsI GAAGAC 1 cut(s) 1602
Bbv12I GWGCWC 3 cut(s) 616, 1417, 1493
BbvI GCAGC 9 cut(s) 88, 217, 280, 320, 1414, 1592, 1672, 1900, 2021
BceAI ACGGC 2 cut(s) 358, 919
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 1819, 1853
BciT130I CCWGG 4 cut(s) 176, 661, 1399, 1539
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 2 cut(s) 15, 1884
BfaI CTAG 2 cut(s) 522, 1059
BfmI CTRYAG 2 cut(s) 438, 1260
BfoI RGCGCY 1 cut(s) 1339
BglII AGATCT 1 cut(s) 1800
BisI GCNGC 9 cut(s) 102, 206, 294, 309, 1403, 1581, 1686, 1914, 2010
BlsI GCNGC 9 cut(s) 103, 207, 295, 310, 1404, 1582, 1687, 1915, 2011
Bme1390I CCNGG 5 cut(s) 98, 176, 661, 1399, 1539
Bme18I GGWCC 2 cut(s) 368, 578
BmeT110I CYCGRG 1 cut(s) 1454
BmgT120I GGNCC 4 cut(s) 368, 517, 578, 836
BmiI GGNNCC 5 cut(s) 370, 519, 580, 838, 1190
BmrFI CCNGG 5 cut(s) 98, 176, 661, 1399, 1539
BmsI GCATC 8 cut(s) 427, 698, 769, 933, 1000, 1045, 1287, 1336
BpiI GAAGAC 1 cut(s) 1602
Bpu10I CCTNAGC 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 98
Bsa29I ATCGAT 1 cut(s) 1560
BsaI GGTCTC 1 cut(s) 1884
BsaJI CCNNGG 6 cut(s) 70, 390, 660, 1537, 1538, 1672
BsaWI WCCGGW 1 cut(s) 1824
BsaXI ACNNNNNCTCC 2 cut(s) 1399, 1429
Bsc4I CCNNNNNNNGG 8 cut(s) 104, 175, 250, 587, 1385, 1817, 2035, 2070
Bse118I RCCGGY 4 cut(s) 104, 365, 786, 1824
Bse1I ACTGG 2 cut(s) 1322, 1442
Bse3DI GCAATG 1 cut(s) 1854
BseBI CCWGG 4 cut(s) 176, 661, 1399, 1539
BseCI ATCGAT 1 cut(s) 1560
BseDI CCNNGG 6 cut(s) 70, 390, 660, 1537, 1538, 1672
BseGI GGATG 8 cut(s) 265, 496, 656, 713, 760, 1060, 1244, 1465
BseLI CCNNNNNNNGG 8 cut(s) 104, 175, 250, 587, 1385, 1817, 2035, 2070
BseMI GCAATG 1 cut(s) 1854
BseMII CTCAG 2 cut(s) 854, 1163
BseNI ACTGG 2 cut(s) 1322, 1442
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 1493
BseXI GCAGC 9 cut(s) 88, 217, 280, 320, 1414, 1592, 1672, 1900, 2021
BsgI GTGCAG 1 cut(s) 1859
Bsh1285I CGRYCG 2 cut(s) 1825, 1829
BshFI GGCC 6 cut(s) 287, 345, 352, 518, 837, 1883
BshTI ACCGGT 1 cut(s) 1824
BshVI ATCGAT 1 cut(s) 1560
BsiEI CGRYCG 2 cut(s) 1825, 1829
BsiHKAI GWGCWC 3 cut(s) 616, 1417, 1493
BsiHKCI CYCGRG 1 cut(s) 1454
BsiSI CCGG 5 cut(s) 98, 105, 366, 787, 1825
BslFI GGGAC 3 cut(s) 354, 1866, 1890
BslI CCNNNNNNNGG 8 cut(s) 104, 175, 250, 587, 1385, 1817, 2035, 2070
BsmAI GTCTC 2 cut(s) 15, 1884
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 3 cut(s) 354, 1866, 1890
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 6 cut(s) 287, 345, 352, 518, 837, 1883
Bso31I GGTCTC 1 cut(s) 1884
BsoBI CYCGRG 1 cut(s) 1454
Bsp1286I GDGCHC 4 cut(s) 616, 1417, 1493, 2041
Bsp1407I TGTACA 4 cut(s) 272, 536, 876, 1790
Bsp143I GATC 5 cut(s) 631, 964, 1109, 1231, 1800
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 1 cut(s) 291
BspANI GGCC 6 cut(s) 287, 345, 352, 518, 837, 1883
BspCNI CTCAG 2 cut(s) 855, 1162
BspDI ATCGAT 1 cut(s) 1560
BspLI GGNNCC 5 cut(s) 370, 519, 580, 838, 1190
BspMAI CTGCAG 2 cut(s) 442, 1264
BspPI GGATC 2 cut(s) 959, 1117
BspQI GCTCTTC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 1884
BsrDI GCAATG 1 cut(s) 1854
BsrFI RCCGGY 4 cut(s) 104, 365, 786, 1824
BsrGI TGTACA 4 cut(s) 272, 536, 876, 1790
BsrI ACTGG 2 cut(s) 1322, 1442
BssAI RCCGGY 4 cut(s) 104, 365, 786, 1824
BssECI CCNNGG 6 cut(s) 70, 390, 660, 1537, 1538, 1672
BssMI GATC 5 cut(s) 631, 964, 1109, 1231, 1800
BssT1I CCWWGG 2 cut(s) 70, 390
Bst2UI CCWGG 4 cut(s) 176, 661, 1399, 1539
Bst4CI ACNGT 2 cut(s) 272, 476
Bst6I CTCTTC 2 cut(s) 111, 1818
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstAUI TGTACA 4 cut(s) 272, 536, 876, 1790
BstC8I GCNNGC 9 cut(s) 106, 123, 354, 560, 616, 948, 952, 1047, 1083
BstDEI CTNAG 6 cut(s) 11, 146, 422, 461, 863, 1149
BstDSI CCRYGG 1 cut(s) 70
BstENI CCTNNNNNAGG 1 cut(s) 1815
BstF5I GGATG 8 cut(s) 265, 496, 656, 713, 760, 1060, 1244, 1465
BstH2I RGCGCY 1 cut(s) 1339
BstHHI GCGC 1 cut(s) 1338
BstKTI GATC 5 cut(s) 634, 967, 1112, 1234, 1803
BstMAI GTCTC 2 cut(s) 15, 1884
BstMBI GATC 5 cut(s) 631, 964, 1109, 1231, 1800
BstMCI CGRYCG 2 cut(s) 1825, 1829
BstMWI GCNNNNNNNGC 5 cut(s) 293, 362, 915, 1055, 1082
BstNI CCWGG 4 cut(s) 176, 661, 1399, 1539
BstNSI RCATGY 3 cut(s) 562, 1587, 1603
BstSCI CCNGG 5 cut(s) 96, 174, 659, 1397, 1537
BstSFI CTRYAG 2 cut(s) 438, 1260
BstSLI GKGCMC 1 cut(s) 1493
BstV1I GCAGC 9 cut(s) 88, 217, 280, 320, 1414, 1592, 1672, 1900, 2021
BstV2I GAAGAC 1 cut(s) 1602
BstX2I RGATCY 3 cut(s) 964, 1109, 1800
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 3 cut(s) 964, 1109, 1800
Bsu15I ATCGAT 1 cut(s) 1560
BsuRI GGCC 6 cut(s) 287, 345, 352, 518, 837, 1883
BsuTUI ATCGAT 1 cut(s) 1560
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 8 cut(s) 265, 496, 656, 713, 760, 1060, 1244, 1465
BtsIMutI CAGTG 1 cut(s) 1095
Cac8I GCNNGC 9 cut(s) 106, 123, 354, 560, 616, 948, 952, 1047, 1083
CaiI CAGNNNCTG 2 cut(s) 211, 1969
CfoI GCGC 1 cut(s) 1338
Cfr10I RCCGGY 4 cut(s) 104, 365, 786, 1824
Cfr13I GGNCC 4 cut(s) 368, 517, 578, 836
ClaI ATCGAT 1 cut(s) 1560
CseI GACGC 2 cut(s) 551, 1312
Csp6I GTAC 8 cut(s) 273, 537, 775, 877, 914, 1115, 1257, 1791
CspAI ACCGGT 1 cut(s) 1824
CspCI CAANNNNNGTGG 2 cut(s) 1407, 1442
CviQI GTAC 8 cut(s) 273, 537, 775, 877, 914, 1115, 1257, 1791
DdeI CTNAG 6 cut(s) 11, 146, 422, 461, 863, 1149
DpnI GATC 5 cut(s) 633, 966, 1111, 1233, 1802
DpnII GATC 5 cut(s) 631, 964, 1109, 1231, 1800
DraI TTTAAA 1 cut(s) 594
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 111, 1818
EarI CTCTTC 2 cut(s) 111, 1818
Ecl136II GAGCTC 1 cut(s) 1415
Eco130I CCWWGG 2 cut(s) 70, 390
Eco24I GRGCYC 2 cut(s) 1417, 2041
Eco31I GGTCTC 1 cut(s) 1884
Eco47I GGWCC 2 cut(s) 368, 578
Eco53kI GAGCTC 1 cut(s) 1415
Eco57I CTGAAG 2 cut(s) 135, 1103
Eco88I CYCGRG 1 cut(s) 1454
EcoICRI GAGCTC 1 cut(s) 1415
EcoNI CCTNNNNNAGG 1 cut(s) 1815
EcoO109I RGGNCCY 2 cut(s) 517, 578
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 4 cut(s) 174, 659, 1397, 1537
EcoT14I CCWWGG 2 cut(s) 70, 390
EcoT38I GRGCYC 2 cut(s) 1417, 2041
ErhI CCWWGG 2 cut(s) 70, 390
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 2 cut(s) 1065, 1097
FaqI GGGAC 3 cut(s) 354, 1866, 1890
FauNDI CATATG 2 cut(s) 277, 1016
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 9 cut(s) 102, 206, 294, 309, 1403, 1581, 1686, 1914, 2010
FokI GGATG 8 cut(s) 272, 503, 643, 720, 747, 1067, 1251, 1472
FriOI GRGCYC 2 cut(s) 1417, 2041
Fsp4HI GCNGC 9 cut(s) 102, 206, 294, 309, 1403, 1581, 1686, 1914, 2010
FspBI CTAG 2 cut(s) 522, 1059
GlaI GCGC 1 cut(s) 1337
GluI GCNGC 9 cut(s) 102, 206, 294, 309, 1403, 1581, 1686, 1914, 2010
HaeII RGCGCY 1 cut(s) 1339
HaeIII GGCC 6 cut(s) 287, 345, 352, 518, 837, 1883
HapII CCGG 5 cut(s) 98, 105, 366, 787, 1825
HgaI GACGC 2 cut(s) 551, 1312
HhaI GCGC 1 cut(s) 1338
Hin6I GCGC 1 cut(s) 1336
HinP1I GCGC 1 cut(s) 1336
HincII GTYRAC 1 cut(s) 792
HindII GTYRAC 1 cut(s) 792
HindIII AAGCTT 3 cut(s) 8, 1074, 1919
HinfI GANTC 5 cut(s) 171, 238, 625, 718, 1638
HpaII CCGG 5 cut(s) 98, 105, 366, 787, 1825
HphI GGTGA 6 cut(s) 158, 275, 327, 835, 1184, 1328
Hpy166II GTNNAC 5 cut(s) 539, 566, 792, 1319, 1491
Hpy188I TCNGA 9 cut(s) 483, 546, 969, 1122, 1152, 1225, 1252, 1673, 2045
Hpy188III TCNNGA 4 cut(s) 629, 1454, 1525, 2071
Hpy8I GTNNAC 5 cut(s) 539, 566, 792, 1319, 1491
Hpy99I CGWCG 1 cut(s) 1306
HpyAV CCTTC 9 cut(s) 20, 297, 325, 569, 1176, 1623, 1675, 2069, 2077
HpyCH4III ACNGT 2 cut(s) 272, 476
HpyF10VI GCNNNNNNNGC 5 cut(s) 293, 362, 915, 1055, 1082
HpyF3I CTNAG 6 cut(s) 11, 146, 422, 461, 863, 1149
HspAI GCGC 1 cut(s) 1336
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 5 cut(s) 631, 964, 1109, 1231, 1800
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 1412, 1420
Lsp1109I GCAGC 9 cut(s) 88, 217, 280, 320, 1414, 1592, 1672, 1900, 2021
LweI GCATC 8 cut(s) 427, 698, 769, 933, 1000, 1045, 1287, 1336
MaeI CTAG 2 cut(s) 522, 1059
MaeIII GTNAC 6 cut(s) 164, 185, 1172, 1193, 1586, 1998
MalI GATC 5 cut(s) 633, 966, 1111, 1233, 1802
MboI GATC 5 cut(s) 631, 964, 1109, 1231, 1800
MboII GAAGA 9 cut(s) 128, 146, 881, 974, 1040, 1491, 1607, 1805, 2017
MfeI CAATTG 1 cut(s) 1937
MflI RGATCY 3 cut(s) 964, 1109, 1800
MhlI GDGCHC 4 cut(s) 616, 1417, 1493, 2041
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 2 cut(s) 1193, 1407
MroNI GCCGGC 1 cut(s) 104
MroXI GAANNNNTTC 3 cut(s) 138, 224, 2024
MseI TTAA 6 cut(s) 450, 593, 938, 984, 1865, 1929
MslI CAYNNNNRTG 7 cut(s) 483, 557, 619, 639, 924, 1098, 1560
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 5 cut(s) 98, 105, 366, 787, 1825
MspR9I CCNGG 5 cut(s) 98, 176, 661, 1399, 1539
MssI GTTTAAAC 1 cut(s) 594
MunI CAATTG 1 cut(s) 1937
Mva1269I GAATGC 1 cut(s) 207
MvaI CCWGG 4 cut(s) 176, 661, 1399, 1539
MwoI GCNNNNNNNGC 5 cut(s) 293, 362, 915, 1055, 1082
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 70
NdeI CATATG 2 cut(s) 277, 1016
NdeII GATC 5 cut(s) 631, 964, 1109, 1231, 1800
NgoMIV GCCGGC 1 cut(s) 104
NlaIV GGNNCC 5 cut(s) 370, 519, 580, 838, 1190
NmeAIII GCCGAG 1 cut(s) 1909
NmuCI GTSAC 4 cut(s) 164, 185, 1172, 1193
NspI RCATGY 3 cut(s) 562, 1587, 1603
OliI CACNNNNGTG 1 cut(s) 619
PaeI GCATGC 1 cut(s) 562
PaeR7I CTCGAG 1 cut(s) 1454
PasI CCCWGGG 1 cut(s) 1538
PciSI GCTCTTC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 1461
PctI GAATGC 1 cut(s) 207
PdiI GCCGGC 1 cut(s) 106
PdmI GAANNNNTTC 3 cut(s) 138, 224, 2024
PfeI GAWTC 4 cut(s) 238, 625, 718, 1638
PflMI CCANNNNNTGG 1 cut(s) 1385
PinAI ACCGGT 1 cut(s) 1824
PkrI GCNGC 9 cut(s) 103, 207, 295, 310, 1404, 1582, 1687, 1915, 2011
PleI GAGTC 1 cut(s) 179
PmeI GTTTAAAC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 179
PpuMI RGGWCCY 1 cut(s) 578
PshBI ATTAAT 2 cut(s) 1865, 1929
Psp124BI GAGCTC 1 cut(s) 1417
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 4 cut(s) 174, 659, 1397, 1537
PspGI CCWGG 4 cut(s) 174, 659, 1397, 1537
PspN4I GGNNCC 5 cut(s) 370, 519, 580, 838, 1190
PspPI GGNCC 4 cut(s) 368, 517, 578, 836
PspPPI RGGWCCY 1 cut(s) 578
PsrI GAACNNNNNNTAC 2 cut(s) 123, 155
PstI CTGCAG 2 cut(s) 442, 1264
PstNI CAGNNNCTG 2 cut(s) 211, 1969
PsuI RGATCY 3 cut(s) 964, 1109, 1800
RsaI GTAC 8 cut(s) 274, 538, 776, 878, 915, 1116, 1258, 1792
RsaNI GTAC 8 cut(s) 273, 537, 775, 877, 914, 1115, 1257, 1791
RseI CAYNNNNRTG 7 cut(s) 483, 557, 619, 639, 924, 1098, 1560
SacI GAGCTC 1 cut(s) 1417
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 6 cut(s) 450, 593, 938, 984, 1865, 1929
SatI GCNGC 9 cut(s) 102, 206, 294, 309, 1403, 1581, 1686, 1914, 2010
Sau3AI GATC 5 cut(s) 631, 964, 1109, 1231, 1800
Sau96I GGNCC 4 cut(s) 368, 517, 578, 836
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 5 cut(s) 98, 176, 661, 1399, 1539
SduI GDGCHC 4 cut(s) 616, 1417, 1493, 2041
SfaNI GCATC 8 cut(s) 427, 698, 769, 933, 1000, 1045, 1287, 1336
SfcI CTRYAG 2 cut(s) 438, 1260
Sfr274I CTCGAG 1 cut(s) 1454
SinI GGWCC 2 cut(s) 368, 578
SlaI CTCGAG 1 cut(s) 1454
SmiMI CAYNNNNRTG 7 cut(s) 483, 557, 619, 639, 924, 1098, 1560
SmlI CTYRAG 1 cut(s) 1454
SmoI CTYRAG 1 cut(s) 1454
SphI GCATGC 1 cut(s) 562
SsiI CCGC 1 cut(s) 291
SspI AATATT 1 cut(s) 2051
SspMI CTAG 2 cut(s) 522, 1059
SstI GAGCTC 1 cut(s) 1417
StyD4I CCNGG 5 cut(s) 96, 174, 659, 1397, 1537
StyI CCWWGG 2 cut(s) 70, 390
TaaI ACNGT 2 cut(s) 272, 476
TaqI TCGA 5 cut(s) 93, 199, 1455, 1560, 1829
TaqII GACCGA 1 cut(s) 1242
TatI WGTACW 7 cut(s) 272, 536, 774, 876, 1114, 1256, 1790
TfiI GAWTC 4 cut(s) 238, 625, 718, 1638
Tru1I TTAA 6 cut(s) 450, 593, 938, 984, 1865, 1929
Tru9I TTAA 6 cut(s) 450, 593, 938, 984, 1865, 1929
TscAI CASTG 1 cut(s) 1102
TseFI GTSAC 4 cut(s) 164, 185, 1172, 1193
TseI GCWGC 9 cut(s) 101, 205, 293, 308, 1402, 1580, 1685, 1913, 2009
Tsp45I GTSAC 4 cut(s) 164, 185, 1172, 1193
TspDTI ATGAA 9 cut(s) 294, 642, 909, 1116, 1146, 1485, 1558, 1636, 1859
TspGWI ACGGA 1 cut(s) 1181
TspRI CASTG 1 cut(s) 1102
Van91I CCANNNNNTGG 1 cut(s) 1385
VneI GTGCAC 1 cut(s) 1489
VpaK11BI GGWCC 2 cut(s) 368, 578
VspI ATTAAT 2 cut(s) 1865, 1929
XagI CCTNNNNNAGG 1 cut(s) 1815
XapI RAATTY 5 cut(s) 897, 1133, 1448, 1733, 1831
XceI RCATGY 3 cut(s) 562, 1587, 1603
XhoI CTCGAG 1 cut(s) 1454
XmiI GTMKAC 1 cut(s) 565
XmnI GAANNNNTTC 3 cut(s) 138, 224, 2024
XspI CTAG 2 cut(s) 522, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.