RchiOBHm_Chr2g0148501

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000173
Physical Location & Seq
Forward (+)
66242571 .. 66250080
7510 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51806

Sequence Viewer

Length: 2286 bp
ATGTGGAAGCTTAGGTTTGGAAATGAAGGTGCAAATAATGACCCTTACAGTCCTTACTTGTTTAGCACAAACAACTTCTATGGGAGACAAACATGGGAGTTTGACTCGGCTGCCGGCACACCTGAAGAGCTAGCAGAGGTCGAAGAAGTTCGACTAAGTTTTTACAACAATCGTCACCAGGTCCAGGCTTGTAGTGACCTCCTCTGCCGAATGCAGCTTCTGGGAGAGAAAAAGTTCAAACAAAAGATTCCCCAAGTAAAGGTGGAGGATGGTGAGGACTGTACATATGAAAAGGCCACCGCTGCATTCAAAAGGGCAGCTACCTTCTACTCTGCCTTGCAATCACCTCACGGCCATTGGCCTGCTGCAAGTGTCGGTCCCATGTTTTATATTCCTTCCTTGGTCATACCGCTCTATATTATGGGGCATCTTAGTAAGGTGTTCTCTGCAGAGCATTATAAGGAAATGCTTAGATATATATACTGTCATCAGAACGAGGATGGTGGGTGGGGATTACACATAGAGGGCCCTAGTATGATGTATTGTACACTTCTGAACTACATTTGCATGCGTCTACTTGGAGAAGGACCTGACGGTGGTTTAAACAACGCTTGTGCAAGAGCACGCAAGTGGATTCTTGATCATGGTGGTGCAACACATTCATCATCCTGGGGAAAGACTTTTATGGCAATACTCGGTGTGTATGAGTGGGAGGGTTGCAACCCGTTTCCCCCAGAGTTTTGGATTTTCTCATCTTTGTTCCCTTTGAATCCAGCAAATATGTTCTGCCATGGCCGGTTGACTTTCTTGCCTATGTCGTATCTGTTTGGGAAAAGATTTGTTGGCCCCATCACCCCTCTCATTCTACAACTGAGAGAAGAAATGTACAATGAGCCATACAGTGAAATTCAATGGGGCAAAGTACGCCATCTCTGTGCAAAGGAGGATAACTACTATCCCCATGGCAAGTTACAATGTTTGTTGTGGGATACTCTTAGCACAGTTTATGAGCCTCTTCTTTCTTGCTGGCCCTTCAATAAGTTGAGAGAGAAGGCTCTTCAAGAAACTATTGAAAACATTCATTATGAAGATGAAAATAGTAGATATATAACTATTGGATGTGTGGAAAAGCCCTTAATGATGCTTGCTTGCTGGATTGAAGATCCGAATGGAGAATCCTTTAAGAAGCATCTTGCAAGAGTTATAGATTACATATGGGTTGCAGAAGATGGAATAACTACGCAGAGTTTTGGTAGCCAGGCATGGGATGCTAGTCTTTCTGTTCAAGCTTTGCTTGCTGGAAATCTCACTGATGAACTTGGATCTGTACTTCAGAAAGCACATGAATTCCTAAACAAATCTCAGGTTAGGGATAATCCTTCTGGTGACTTCTTAGCTCGTTTCCGTCACATTTCTAAAGGTAGTTGGACTTTCTCTGACCAAGATCATGGATGGCAAGTGTCTGATTGTACTGCAGAGAGTTTGAAGTGTTGCCTACTGTTTTCAATGATGCCGACGCAAATCGTGGGTGAACCAATTGACTCTGAGCGCTTGTATGATGCTGTTAATGTCATACTTTCTCTGCAAAATTCAAATGGTGGTGTATCAGCCTGGCAGCCAACAGGAGCTCCACAATGGTTGGAGTGGTTCAATCCAGTAGAATTTCTCGAGAATGTCGTCATCGAATACGAGTATGTTGAGTGCACTTCATCTTCAATTCAAGCGTTAACTTTATTCAGGAAGTTATACCCTGGGCATAGAAAGAAACATATCGATGATTTCATCACAAATGCAGCATGTTACCTTGAAGACATGCAAACACCTGATGGCTCATGGTATGGAAAATGGGGAATCTGCTTCATCTATGGAACATGGTTTGCAATTCTCGGGTTGAAGGCTGCTGGTAGAACTTACAAAAATTGTGAGGCGATACGCAGAGGTGTTGAATTTTTACTCAAAATACAGAGAAATGATGGTGGTTGGGGAGAGAGCTACACTTCTTGTACCAACAAGATCTATACACCTCTTGAAGGCGAACGGTCGAATTTGGTGCAGACTGCAATGGGACTAATGGGATTAATTCATGGTGGACAGGCCGAGAGAGACCCTACTCCCATTCACAGAGCTGCAAAGCTTTTGATTAATTCTCAATTGGAAAATGGAGATTTCCCCCAACAGGAACTGGTGGGGGCTTTCAATGTTAATGCCATGTTACACTATGCAGCATACAGGAATATCTTCCCAATTTGGGCTCTCGGAGAATATCGTTCCCTTGCCTCCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

761

Amino Acids

86.72

Weight (kDa)

5.65

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 104 - 365 1.5e-33 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 419 - 756 1.5e-47 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AccBSI CCGCTC 1 cut(s) 412
AccI GTMKAC 1 cut(s) 574
AciI CCGC 2 cut(s) 300, 410
AclWI GGATC 2 cut(s) 1157, 1330
AcoI YGGCCR 2 cut(s) 352, 793
AcsI RAATTY 6 cut(s) 906, 1346, 1588, 1661, 1946, 2044
AcuI CTGAAG 2 cut(s) 144, 1316
AfaI GTAC 7 cut(s) 283, 547, 887, 924, 1329, 1471, 2005
AfeI AGCGCT 1 cut(s) 1550
AfiI CCNNNNNNNGG 7 cut(s) 184, 259, 596, 1264, 2030, 2176, 2248
AjnI CCWGG 6 cut(s) 177, 183, 668, 1257, 1610, 1750
AleI CACNNNNGTG 1 cut(s) 628
Alw21I GWGCWC 3 cut(s) 625, 1630, 1706
Alw26I GTCTC 2 cut(s) 79, 2097
Alw44I GTGCAC 1 cut(s) 1702
AlwI GGATC 2 cut(s) 1157, 1330
AlwNI CAGNNNCTG 2 cut(s) 220, 2182
Ama87I CYCGRG 2 cut(s) 1667, 1886
Aor51HI AGCGCT 1 cut(s) 1550
AoxI GGCC 8 cut(s) 294, 352, 359, 526, 793, 844, 1028, 2094
ApaI GGGCCC 1 cut(s) 530
ApaLI GTGCAC 1 cut(s) 1702
ApoI RAATTY 6 cut(s) 906, 1346, 1588, 1661, 1946, 2044
AseI ATTAAT 2 cut(s) 2078, 2142
Asp700I GAANNNNTTC 4 cut(s) 147, 233, 1077, 2237
AspLEI GCGC 1 cut(s) 1551
AspS9I GGNCC 7 cut(s) 181, 377, 526, 527, 587, 845, 1029
AsuHPI GGTGA 6 cut(s) 167, 284, 336, 844, 1397, 1541
AsuNHI GCTAGC 1 cut(s) 130
AvaI CYCGRG 2 cut(s) 1667, 1886
AvaII GGWCC 3 cut(s) 181, 377, 587
BaeGI GKGCMC 2 cut(s) 530, 1706
BanII GRGCYC 3 cut(s) 530, 1630, 2254
BbsI GAAGAC 1 cut(s) 1815
Bbv12I GWGCWC 3 cut(s) 625, 1630, 1706
BccI CCATC 8 cut(s) 263, 494, 857, 936, 1223, 1446, 1820, 1967
BceAI ACGGC 1 cut(s) 367
BcgI CGANNNNNNTGC 2 cut(s) 2032, 2066
BciT130I CCWGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
BciVI GTATCC 1 cut(s) 982
BclI TGATCA 1 cut(s) 640
BcoDI GTCTC 2 cut(s) 79, 2097
BfaI CTAG 4 cut(s) 131, 531, 1272, 2284
BfmI CTRYAG 2 cut(s) 447, 1473
BfoI RGCGCY 1 cut(s) 1552
BfuI GTATCC 1 cut(s) 982
BglII AGATCT 1 cut(s) 2013
Bme1390I CCNGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
Bme18I GGWCC 3 cut(s) 181, 377, 587
BmeT110I CYCGRG 2 cut(s) 1667, 1886
BmgT120I GGNCC 7 cut(s) 181, 377, 526, 527, 587, 845, 1029
BmiI GGNNCC 3 cut(s) 379, 528, 847
BmrFI CCNGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
BmsI GCATC 6 cut(s) 436, 1131, 1198, 1258, 1500, 1549
BmtI GCTAGC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 1815
BplI GAGNNNNNCTC 2 cut(s) 89, 121
Bpu10I CCTNAGC 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 1773
BsaI GGTCTC 1 cut(s) 2097
BsaJI CCNNGG 6 cut(s) 399, 669, 790, 961, 1750, 1751
BsaXI ACNNNNNCTCC 2 cut(s) 1612, 1642
Bsc4I CCNNNNNNNGG 7 cut(s) 184, 259, 596, 1264, 2030, 2176, 2248
Bse118I RCCGGY 2 cut(s) 113, 795
Bse1I ACTGG 2 cut(s) 1655, 2187
Bse3DI GCAATG 1 cut(s) 2067
BseBI CCWGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
BseCI ATCGAT 1 cut(s) 1773
BseDI CCNNGG 6 cut(s) 399, 669, 790, 961, 1750, 1751
BseGI GGATG 6 cut(s) 274, 505, 665, 1124, 1273, 1457
BseLI CCNNNNNNNGG 7 cut(s) 184, 259, 596, 1264, 2030, 2176, 2248
BseMI GCAATG 1 cut(s) 2067
BseMII CTCAG 3 cut(s) 863, 1376, 1536
BseNI ACTGG 2 cut(s) 1655, 2187
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 2 cut(s) 530, 1706
BsgI GTGCAG 1 cut(s) 2072
Bsh1285I CGRYCG 1 cut(s) 2042
BshFI GGCC 8 cut(s) 296, 354, 361, 528, 795, 846, 1030, 2096
BshVI ATCGAT 1 cut(s) 1773
BsiEI CGRYCG 1 cut(s) 2042
BsiHKAI GWGCWC 3 cut(s) 625, 1630, 1706
BsiHKCI CYCGRG 2 cut(s) 1667, 1886
BsiSI CCGG 2 cut(s) 114, 796
BslFI GGGAC 2 cut(s) 363, 2079
BslI CCNNNNNNNGG 7 cut(s) 184, 259, 596, 1264, 2030, 2176, 2248
BsmAI GTCTC 2 cut(s) 79, 2097
BsmFI GGGAC 2 cut(s) 363, 2079
BsmI GAATGC 2 cut(s) 216, 305
BsnI GGCC 8 cut(s) 296, 354, 361, 528, 795, 846, 1030, 2096
Bso31I GGTCTC 1 cut(s) 2097
BsoBI CYCGRG 2 cut(s) 1667, 1886
Bsp120I GGGCCC 1 cut(s) 526
Bsp1286I GDGCHC 5 cut(s) 530, 625, 1630, 1706, 2254
Bsp1407I TGTACA 3 cut(s) 281, 545, 885
Bsp143I GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
Bsp19I CCATGG 2 cut(s) 790, 961
BspACI CCGC 2 cut(s) 300, 410
BspANI GGCC 8 cut(s) 296, 354, 361, 528, 795, 846, 1030, 2096
BspCNI CTCAG 3 cut(s) 864, 1375, 1537
BspDI ATCGAT 1 cut(s) 1773
BspLI GGNNCC 3 cut(s) 379, 528, 847
BspMAI CTGCAG 2 cut(s) 451, 1477
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 1157, 1330
BspQI GCTCTTC 2 cut(s) 120, 1062
BspTNI GGTCTC 1 cut(s) 2097
BsrBI CCGCTC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 2067
BsrFI RCCGGY 2 cut(s) 113, 795
BsrGI TGTACA 3 cut(s) 281, 545, 885
BsrI ACTGG 2 cut(s) 1655, 2187
BssAI RCCGGY 2 cut(s) 113, 795
BssECI CCNNGG 6 cut(s) 399, 669, 790, 961, 1750, 1751
BssMI GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
BssT1I CCWWGG 3 cut(s) 399, 790, 961
Bst2UI CCWGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
Bst4CI ACNGT 8 cut(s) 50, 281, 485, 596, 902, 1003, 1500, 2040
Bst6I CTCTTC 3 cut(s) 120, 1020, 1062
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstAUI TGTACA 3 cut(s) 281, 545, 885
BstC8I GCNNGC 9 cut(s) 115, 132, 363, 569, 625, 1028, 1146, 1150, 1296
BstDEI CTNAG 9 cut(s) 11, 155, 431, 470, 872, 995, 1362, 1393, 1545
BstDSI CCRYGG 2 cut(s) 790, 961
BstENI CCTNNNNNAGG 1 cut(s) 2028
BstF5I GGATG 6 cut(s) 274, 505, 665, 1124, 1273, 1457
BstH2I RGCGCY 1 cut(s) 1552
BstHHI GCGC 1 cut(s) 1551
BstKTI GATC 5 cut(s) 643, 1165, 1325, 1447, 2016
BstMAI GTCTC 2 cut(s) 79, 2097
BstMBI GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
BstMCI CGRYCG 1 cut(s) 2042
BstMWI GCNNNNNNNGC 4 cut(s) 302, 924, 1268, 1295
BstNI CCWGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
BstNSI RCATGY 3 cut(s) 571, 1800, 1816
BstSCI CCNGG 6 cut(s) 177, 183, 668, 1257, 1610, 1750
BstSFI CTRYAG 2 cut(s) 447, 1473
BstSLI GKGCMC 2 cut(s) 530, 1706
BstV2I GAAGAC 1 cut(s) 1815
BstX2I RGATCY 3 cut(s) 1162, 1322, 2013
BstXI CCANNNNNNTGG 2 cut(s) 741, 1448
BstYI RGATCY 3 cut(s) 1162, 1322, 2013
Bsu15I ATCGAT 1 cut(s) 1773
BsuI GTATCC 1 cut(s) 982
BsuRI GGCC 8 cut(s) 296, 354, 361, 528, 795, 846, 1030, 2096
BsuTUI ATCGAT 1 cut(s) 1773
BtgI CCRYGG 2 cut(s) 790, 961
BtsCI GGATG 6 cut(s) 274, 505, 665, 1124, 1273, 1457
BtsIMutI CAGTG 2 cut(s) 907, 1308
Cac8I GCNNGC 9 cut(s) 115, 132, 363, 569, 625, 1028, 1146, 1150, 1296
CaiI CAGNNNCTG 2 cut(s) 220, 2182
CfoI GCGC 1 cut(s) 1551
Cfr10I RCCGGY 2 cut(s) 113, 795
Cfr13I GGNCC 7 cut(s) 181, 377, 526, 527, 587, 845, 1029
ClaI ATCGAT 1 cut(s) 1773
CseI GACGC 2 cut(s) 560, 1525
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 7 cut(s) 282, 546, 886, 923, 1328, 1470, 2004
CspCI CAANNNNNGTGG 2 cut(s) 1620, 1655
CviQI GTAC 7 cut(s) 282, 546, 886, 923, 1328, 1470, 2004
DdeI CTNAG 9 cut(s) 11, 155, 431, 470, 872, 995, 1362, 1393, 1545
DpnI GATC 5 cut(s) 642, 1164, 1324, 1446, 2015
DpnII GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
DraI TTTAAA 1 cut(s) 603
EaeI YGGCCR 2 cut(s) 352, 793
Eam1104I CTCTTC 3 cut(s) 120, 1020, 1062
EarI CTCTTC 3 cut(s) 120, 1020, 1062
Ecl136II GAGCTC 1 cut(s) 1628
Eco130I CCWWGG 3 cut(s) 399, 790, 961
Eco24I GRGCYC 3 cut(s) 530, 1630, 2254
Eco31I GGTCTC 1 cut(s) 2097
Eco47I GGWCC 3 cut(s) 181, 377, 587
Eco47III AGCGCT 1 cut(s) 1550
Eco53kI GAGCTC 1 cut(s) 1628
Eco57I CTGAAG 2 cut(s) 144, 1316
Eco88I CYCGRG 2 cut(s) 1667, 1886
EcoICRI GAGCTC 1 cut(s) 1628
EcoNI CCTNNNNNAGG 1 cut(s) 2028
EcoO109I RGGNCCY 3 cut(s) 526, 527, 587
EcoRI GAATTC 1 cut(s) 1346
EcoRII CCWGG 6 cut(s) 177, 183, 668, 1257, 1610, 1750
EcoT14I CCWWGG 3 cut(s) 399, 790, 961
EcoT38I GRGCYC 3 cut(s) 530, 1630, 2254
ErhI CCWWGG 3 cut(s) 399, 790, 961
FalI AAGNNNNNCTT 2 cut(s) 1278, 1310
FaqI GGGAC 2 cut(s) 363, 2079
FauNDI CATATG 2 cut(s) 286, 1214
FbaI TGATCA 1 cut(s) 640
FblI GTMKAC 1 cut(s) 574
FokI GGATG 6 cut(s) 281, 512, 652, 1131, 1280, 1464
FriOI GRGCYC 3 cut(s) 530, 1630, 2254
FspBI CTAG 4 cut(s) 131, 531, 1272, 2284
GlaI GCGC 1 cut(s) 1550
HaeII RGCGCY 1 cut(s) 1552
HaeIII GGCC 8 cut(s) 296, 354, 361, 528, 795, 846, 1030, 2096
HapII CCGG 2 cut(s) 114, 796
HgaI GACGC 2 cut(s) 560, 1525
HhaI GCGC 1 cut(s) 1551
Hin6I GCGC 1 cut(s) 1549
HinP1I GCGC 1 cut(s) 1549
HincII GTYRAC 2 cut(s) 801, 1728
HindII GTYRAC 2 cut(s) 801, 1728
HindIII AAGCTT 3 cut(s) 8, 1287, 2132
HinfI GANTC 7 cut(s) 104, 247, 634, 769, 1175, 1541, 1851
HpaI GTTAAC 1 cut(s) 1728
HpaII CCGG 2 cut(s) 114, 796
HphI GGTGA 6 cut(s) 167, 284, 336, 844, 1397, 1541
Hpy166II GTNNAC 7 cut(s) 548, 575, 801, 1532, 1704, 1728, 2090
Hpy188I TCNGA 8 cut(s) 492, 555, 1167, 1335, 1438, 1465, 1546, 2258
Hpy188III TCNNGA 6 cut(s) 638, 1061, 1667, 1669, 1738, 2027
Hpy8I GTNNAC 7 cut(s) 548, 575, 801, 1532, 1704, 1728, 2090
Hpy99I CGWCG 1 cut(s) 1519
HpyAV CCTTC 9 cut(s) 20, 334, 405, 578, 1042, 1045, 1389, 1888, 2024
HpyCH4III ACNGT 8 cut(s) 50, 281, 485, 596, 902, 1003, 1500, 2040
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 924, 1268, 1295
HpyF3I CTNAG 9 cut(s) 11, 155, 431, 470, 872, 995, 1362, 1393, 1545
HspAI GCGC 1 cut(s) 1549
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 640
KspAI GTTAAC 1 cut(s) 1728
Kzo9I GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
LguI GCTCTTC 2 cut(s) 120, 1062
LmnI GCTCC 2 cut(s) 1625, 1633
LweI GCATC 6 cut(s) 436, 1131, 1198, 1258, 1500, 1549
MabI ACCWGGT 1 cut(s) 177
MaeI CTAG 4 cut(s) 131, 531, 1272, 2284
MaeIII GTNAC 7 cut(s) 173, 194, 969, 1385, 1406, 1799, 2211
MalI GATC 5 cut(s) 642, 1164, 1324, 1446, 2015
MbiI CCGCTC 1 cut(s) 412
MboI GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
MfeI CAATTG 2 cut(s) 1536, 2150
MflI RGATCY 3 cut(s) 1162, 1322, 2013
MhlI GDGCHC 5 cut(s) 530, 625, 1630, 1706, 2254
MlyI GAGTC 2 cut(s) 98, 1535
MmeI TCCRAC 2 cut(s) 1406, 1620
MroNI GCCGGC 1 cut(s) 113
MroXI GAANNNNTTC 4 cut(s) 147, 233, 1077, 2237
MseI TTAA 8 cut(s) 602, 1136, 1182, 1566, 1727, 2078, 2142, 2202
MslI CAYNNNNRTG 5 cut(s) 566, 628, 648, 933, 1773
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 2 cut(s) 114, 796
MspR9I CCNGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
MssI GTTTAAAC 1 cut(s) 603
MunI CAATTG 2 cut(s) 1536, 2150
Mva1269I GAATGC 2 cut(s) 216, 305
MvaI CCWGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
MwoI GCNNNNNNNGC 4 cut(s) 302, 924, 1268, 1295
NaeI GCCGGC 1 cut(s) 115
NcoI CCATGG 2 cut(s) 790, 961
NdeI CATATG 2 cut(s) 286, 1214
NdeII GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
NgoMIV GCCGGC 1 cut(s) 113
NheI GCTAGC 1 cut(s) 130
NlaIV GGNNCC 3 cut(s) 379, 528, 847
NmeAIII GCCGAG 2 cut(s) 86, 2122
NmuCI GTSAC 4 cut(s) 173, 194, 1385, 1406
NspI RCATGY 3 cut(s) 571, 1800, 1816
OliI CACNNNNGTG 1 cut(s) 628
PaeI GCATGC 1 cut(s) 571
PaeR7I CTCGAG 1 cut(s) 1667
PasI CCCWGGG 1 cut(s) 1751
PciSI GCTCTTC 2 cut(s) 120, 1062
PcsI WCGNNNNNNNCGW 1 cut(s) 1674
PctI GAATGC 2 cut(s) 216, 305
PdiI GCCGGC 1 cut(s) 115
PdmI GAANNNNTTC 4 cut(s) 147, 233, 1077, 2237
PfeI GAWTC 5 cut(s) 247, 634, 769, 1175, 1851
PleI GAGTC 2 cut(s) 98, 1535
PmeI GTTTAAAC 1 cut(s) 603
PpsI GAGTC 2 cut(s) 98, 1535
PpuMI RGGWCCY 1 cut(s) 587
PshBI ATTAAT 2 cut(s) 2078, 2142
PsiI TTATAA 1 cut(s) 459
Psp124BI GAGCTC 1 cut(s) 1630
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 6 cut(s) 177, 183, 668, 1257, 1610, 1750
PspGI CCWGG 6 cut(s) 177, 183, 668, 1257, 1610, 1750
PspN4I GGNNCC 3 cut(s) 379, 528, 847
PspOMI GGGCCC 1 cut(s) 526
PspPI GGNCC 7 cut(s) 181, 377, 526, 527, 587, 845, 1029
PspPPI RGGWCCY 1 cut(s) 587
PstI CTGCAG 2 cut(s) 451, 1477
PstNI CAGNNNCTG 2 cut(s) 220, 2182
PsuI RGATCY 3 cut(s) 1162, 1322, 2013
RsaI GTAC 7 cut(s) 283, 547, 887, 924, 1329, 1471, 2005
RsaNI GTAC 7 cut(s) 282, 546, 886, 923, 1328, 1470, 2004
RseI CAYNNNNRTG 5 cut(s) 566, 628, 648, 933, 1773
SacI GAGCTC 1 cut(s) 1630
SapI GCTCTTC 2 cut(s) 120, 1062
SaqAI TTAA 8 cut(s) 602, 1136, 1182, 1566, 1727, 2078, 2142, 2202
Sau3AI GATC 5 cut(s) 640, 1162, 1322, 1444, 2013
Sau96I GGNCC 7 cut(s) 181, 377, 526, 527, 587, 845, 1029
SchI GAGTC 2 cut(s) 98, 1535
ScrFI CCNGG 6 cut(s) 179, 185, 670, 1259, 1612, 1752
SduI GDGCHC 5 cut(s) 530, 625, 1630, 1706, 2254
SexAI ACCWGGT 1 cut(s) 177
SfaNI GCATC 6 cut(s) 436, 1131, 1198, 1258, 1500, 1549
SfcI CTRYAG 2 cut(s) 447, 1473
Sfr274I CTCGAG 1 cut(s) 1667
SinI GGWCC 3 cut(s) 181, 377, 587
SlaI CTCGAG 1 cut(s) 1667
SmiMI CAYNNNNRTG 5 cut(s) 566, 628, 648, 933, 1773
SmlI CTYRAG 1 cut(s) 1667
SmoI CTYRAG 1 cut(s) 1667
SphI GCATGC 1 cut(s) 571
SsiI CCGC 2 cut(s) 300, 410
SspMI CTAG 4 cut(s) 131, 531, 1272, 2284
SstI GAGCTC 1 cut(s) 1630
StyD4I CCNGG 6 cut(s) 177, 183, 668, 1257, 1610, 1750
StyI CCWWGG 3 cut(s) 399, 790, 961
TaaI ACNGT 8 cut(s) 50, 281, 485, 596, 902, 1003, 1500, 2040
TaqI TCGA 6 cut(s) 141, 151, 1668, 1683, 1773, 2042
TaqII GACCGA 1 cut(s) 365
TatI WGTACW 5 cut(s) 281, 545, 885, 1327, 1469
TfiI GAWTC 5 cut(s) 247, 634, 769, 1175, 1851
Tru1I TTAA 8 cut(s) 602, 1136, 1182, 1566, 1727, 2078, 2142, 2202
Tru9I TTAA 8 cut(s) 602, 1136, 1182, 1566, 1727, 2078, 2142, 2202
TscAI CASTG 2 cut(s) 907, 1315
TseFI GTSAC 4 cut(s) 173, 194, 1385, 1406
Tsp45I GTSAC 4 cut(s) 173, 194, 1385, 1406
TspGWI ACGGA 1 cut(s) 1394
TspRI CASTG 2 cut(s) 907, 1315
VneI GTGCAC 1 cut(s) 1702
VpaK11BI GGWCC 3 cut(s) 181, 377, 587
VspI ATTAAT 2 cut(s) 2078, 2142
XagI CCTNNNNNAGG 1 cut(s) 2028
XapI RAATTY 6 cut(s) 906, 1346, 1588, 1661, 1946, 2044
XceI RCATGY 3 cut(s) 571, 1800, 1816
XhoI CTCGAG 1 cut(s) 1667
XmiI GTMKAC 1 cut(s) 574
XmnI GAANNNNTTC 4 cut(s) 147, 233, 1077, 2237
XspI CTAG 4 cut(s) 131, 531, 1272, 2284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.