MD09G1168200.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000173
Physical Location & Seq
Reverse (-)
13869658 .. 13876574
6917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1168200.v1.1.491

Sequence Viewer

Length: 2289 bp
ATGTGGAAGCTTAAGGTCGCAGATGGGGGCAATGATCCATACATCTACAGCACAAACAACTTCGTGGGAAGGCAGATATTTGAGTTTGATCCTGAAGCAGGAACTACTGAAGAGAGAGCTGAGGTGGAAGAAGCTCGTCTTCATTTCTACAATAACCGCTATCAGGTTAAGCCCAGTGGTGACTTCCTATGGCGAATGCAGTTCCTAAGGGAGAAGAACTTTAAACAAACAATTCCCCCAATTAAAGTTGAGGATGGAGAGGAAATCACACATGAAAAGGCCACAGCTTCATTGAGAAGGTCTGTCCACTTCTTTTCAGCGTTGCAGGCTAGCGATGGCCATTGGCCTGCCGAAAATGCCGGCCCGTTGTTTTTCCTTCCTCCTTTGGTAATGTGTGTTTACATTACAGGGCATCTTAACACTGTATTCCATGCAGAACACCGTAAAGAAATTCTGCGTTACATATACTATCATCAGAACCAAGATGGTGGTTGGGGATTACACATTGAAGGACACAGCACTATGTTCTGCACAGCTCTCAGCTACATTTGTATGCGTATTCTTGGAGAAGGACCTGATGGTGGCCAAGACAATGCTTGTGCAAGAGCTAGAAAGTGGATTCTTGATCATGGCAGTGTTACCCACATCCCCTCATGGGGTAAAACTTGGCTTTCGATACTTGGTGTGTTTGAGTGGTCCGGAAGCAACCCAATGCCCCCAGAGTTTTGGATTCTGCCTTCATTTCTTCCCATGCATCCAGCAAAAATGTGGTGCTACTGTAGGATGGTTTACATGCCTATGTCATATTTATATGGGAAAAGATTTGTTGGTCCAATCACACCTCTCATACTACAATTAAGGGAAGAACTCTATGCTCAGCCTTATGACGAAATTAACTGGAAGGGAGTGCGTCATCACTGTGCTAAAGAGGACATCTACTACCCTCATCCTTGGATACAGGACTTTCTATGGGATAGTCTGTACATATGTACGGAGCCTCTTCTTACTCGCTGGCCATTTAACAAGATGATCAGGAAAAGGGCTCTCGAAGTAACAATGAAGCACATTCATTATGAAGATGAAAACAGCAGATACATTACCATTGGATGTGTCGAAAAGGTGTTATGCATGCTGGCTTGTTGGGCTGAAGACCCAAATGGTGATTATTTTAAAAAGCATCTTGCCAGGATACCAGATTATTTATGGGTTGCTGAGGATGGGATGAAGATGCAAAGTTTTGGCAGTCAACAGTGGGATACTGGTTTTGCCATTCAAGCTTTGCTTGCTTCCAATCTAACCGATGAAATAGCACCCACACTTGCTAGAGGACATGACTTCGTCAAGAAATCCCAGGTTAAGGACAATCCATCGGGTGACTTCAAAAGCATGCACCGCCACATTTCCAAAGGATCGTGGACTTTTTCTCTGATCAAGACCATGGATGGCAAGTTTCTGATTGCACTGCAGAGGGTTTTAAAGTGTTGCCTGCTTTTATCGATGATGCCACCGGAGCTGGTTGGTGAAAAAATGGAACCTGAGCGGTTATACGATGCTGTCAATGTCCTAATTTCCCTGCAGAGTAAAAATGGTGGTTTAGCAGCCTGGGAACCAGCTGGAGCAGCAGAATGGTTAGAAATGTTGAATCCCACGGAATTCTTCGCGGACATTGTTGTTGAGCATGAATATGTTGAATGCACTTCATCAGCAATTCAGGCTTTAGTTTTGTTTAAGAAGTTGTACCCTGGCCACAGGAAGAAAGAGATAGATCAATTTATTACCAATGCTGCACAGTACCTTGAAAATATACAAATGGAAGATGGTTCATGGTACGGGAATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCTCTTGGAGGGTTAACAGCTGCTGGCAAGACTTTCAACAACTGTGCAGTTATACGCAAAGCTATTAATTTTCTACTCACAATCCAGAAAGAGAACGGTGGTTGGGGAGAGAGCTACCTTTCATGTCCAAAAAAGGAGTATGTTCCTCTTGAAGGAAACCGATCAAATTTAGTACACACAGCTTGGGCTATGATGGGTTTGATTTATGCAGGACAGGCCGAAAGAGACCCCGCACCTCTTCATCGTGCAGCAAAGTTGATAATGAACTCTCAGATGGAAAATGGTGATTTTTCTCAGCAGGAAATCACTGGAGTGTTCAATAAGAATTGTATGCTACATTATGCAGCTTATAGAAACATCTACCCCCTGTGGGCTCTTGCAGAATACCGCAAGTGGGTTCCATTGCCTTCCAAAGCGTGA

Protein Analysis

763

Amino Acids

87.69

Weight (kDa)

6.46

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 99 - 402 1.1e-35 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 417 - 754 1.3e-50 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1859
AccB1I GGYRCC 1 cut(s) 1859
AccBSI CCGCTC 1 cut(s) 1540
AccII CGCG 1 cut(s) 1661
AccIII TCCGGA 1 cut(s) 698
AciI CCGC 6 cut(s) 157, 1393, 1540, 1661, 2100, 2257
AclWI GGATC 3 cut(s) 29, 83, 1417
AcoI YGGCCR 4 cut(s) 337, 583, 1013, 1744
AcsI RAATTY 3 cut(s) 450, 1652, 2035
AcuI CTGAAG 3 cut(s) 114, 129, 1167
AfaI GTAC 7 cut(s) 983, 991, 1739, 1793, 1829, 1861, 2043
AfiI CCNNNNNNNGG 9 cut(s) 98, 163, 581, 655, 656, 1357, 2021, 2239, 2263
AflII CTTAAG 1 cut(s) 11
AgsI TTSAA 9 cut(s) 509, 1274, 1381, 1642, 1691, 1799, 1906, 2021, 2188
AjnI CCWGG 5 cut(s) 1184, 1350, 1601, 1741, 1862
AleI CACNNNNGTG 1 cut(s) 2180
Alw26I GTCTC 1 cut(s) 2088
AlwI GGATC 3 cut(s) 29, 83, 1417
AlwNI CAGNNNCTG 1 cut(s) 1892
Aor13HI TCCGGA 1 cut(s) 698
AoxI GGCC 8 cut(s) 279, 337, 344, 361, 583, 1013, 1744, 2085
ApeKI GCWGC 6 cut(s) 1598, 1619, 1784, 1889, 2117, 2213
ApoI RAATTY 3 cut(s) 450, 1652, 2035
AseI ATTAAT 1 cut(s) 1935
Asp718I GGTACC 1 cut(s) 1859
AspS9I GGNCC 4 cut(s) 362, 572, 696, 830
AsuHPI GGTGA 5 cut(s) 191, 1172, 1385, 1532, 2165
AsuNHI GCTAGC 1 cut(s) 329
AvaII GGWCC 3 cut(s) 572, 696, 830
AxyI CCTNAGG 1 cut(s) 206
BalI TGGCCA 4 cut(s) 339, 585, 1015, 1746
BanI GGYRCC 1 cut(s) 1859
BanII GRGCYC 2 cut(s) 1045, 2245
BbsI GAAGAC 2 cut(s) 131, 1155
BbvCI CCTCAGC 2 cut(s) 120, 1212
BbvI GCAGC 6 cut(s) 1610, 1631, 1771, 1876, 2129, 2225
BciT130I CCWGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
BciVI GTATCC 3 cut(s) 948, 1182, 1249
BclI TGATCA 3 cut(s) 625, 1029, 1428
BcoDI GTCTC 1 cut(s) 2088
BfaI CTAG 3 cut(s) 330, 609, 1323
BfmI CTRYAG 4 cut(s) 46, 778, 1463, 1574
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 3 cut(s) 948, 1182, 1249
BisI GCNGC 6 cut(s) 1599, 1620, 1785, 1890, 2118, 2214
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 6 cut(s) 1600, 1621, 1786, 1891, 2119, 2215
Bme1390I CCNGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
Bme18I GGWCC 3 cut(s) 572, 696, 830
BmgT120I GGNCC 4 cut(s) 362, 572, 696, 830
BmiI GGNNCC 5 cut(s) 996, 1533, 1608, 1861, 2268
BmrFI CCNGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 6 cut(s) 421, 763, 1186, 1218, 1491, 1540
BmtI GCTAGC 1 cut(s) 333
BmuI ACTGGG 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 131, 1155
BpmI CTGGAG 2 cut(s) 1635, 2199
Bpu10I CCTNAGC 3 cut(s) 120, 1212, 1536
Bpu1102I GCTNAGC 1 cut(s) 876
Bsa29I ATCGAT 1 cut(s) 1496
BsaI GGTCTC 1 cut(s) 2088
BsaJI CCNNGG 6 cut(s) 950, 1350, 1437, 1602, 1647, 1741
BsaWI WCCGGW 2 cut(s) 698, 1507
Bsc4I CCNNNNNNNGG 9 cut(s) 98, 163, 581, 655, 656, 1357, 2021, 2239, 2263
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 4 cut(s) 174, 902, 1264, 2182
Bse21I CCTNAGG 1 cut(s) 206
Bse3DI GCAATG 2 cut(s) 37, 2270
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
BseCI ATCGAT 1 cut(s) 1496
BseDI CCNNGG 6 cut(s) 950, 1350, 1437, 1602, 1647, 1741
BseGI GGATG 9 cut(s) 259, 645, 754, 789, 946, 1112, 1222, 1227, 1447
BseLI CCNNNNNNNGG 9 cut(s) 98, 163, 581, 655, 656, 1357, 2021, 2239, 2263
BseMI GCAATG 2 cut(s) 37, 2270
BseMII CTCAG 7 cut(s) 111, 553, 890, 1203, 1527, 2153, 2177
BseNI ACTGG 4 cut(s) 174, 902, 1264, 2182
BseXI GCAGC 6 cut(s) 1610, 1631, 1771, 1876, 2129, 2225
BsgI GTGCAG 4 cut(s) 514, 1770, 1935, 2136
Bsh1236I CGCG 1 cut(s) 1661
BshFI GGCC 8 cut(s) 281, 339, 346, 363, 585, 1015, 1746, 2087
BshNI GGYRCC 1 cut(s) 1859
BshVI ATCGAT 1 cut(s) 1496
BsiSI CCGG 3 cut(s) 360, 699, 1508
BslI CCNNNNNNNGG 9 cut(s) 98, 163, 581, 655, 656, 1357, 2021, 2239, 2263
BsmAI GTCTC 1 cut(s) 2088
BsmI GAATGC 2 cut(s) 201, 1697
BsnI GGCC 8 cut(s) 281, 339, 346, 363, 585, 1015, 1746, 2087
Bso31I GGTCTC 1 cut(s) 2088
Bsp1286I GDGCHC 2 cut(s) 1045, 2245
Bsp13I TCCGGA 1 cut(s) 698
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
Bsp1720I GCTNAGC 1 cut(s) 876
Bsp19I CCATGG 1 cut(s) 1437
BspACI CCGC 6 cut(s) 157, 1393, 1540, 1661, 2100, 2257
BspANI GGCC 8 cut(s) 281, 339, 346, 363, 585, 1015, 1746, 2087
BspCNI CTCAG 7 cut(s) 112, 552, 889, 1204, 1528, 2152, 2176
BspDI ATCGAT 1 cut(s) 1496
BspEI TCCGGA 1 cut(s) 698
BspFNI CGCG 1 cut(s) 1661
BspLI GGNNCC 5 cut(s) 996, 1533, 1608, 1861, 2268
BspMAI CTGCAG 2 cut(s) 1467, 1578
BspOI GCTAGC 1 cut(s) 333
BspPI GGATC 3 cut(s) 29, 83, 1417
BspT107I GGYRCC 1 cut(s) 1859
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 2088
BsrBI CCGCTC 1 cut(s) 1540
BsrDI GCAATG 2 cut(s) 37, 2270
BsrFI RCCGGY 1 cut(s) 359
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 4 cut(s) 174, 902, 1264, 2182
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 6 cut(s) 950, 1350, 1437, 1602, 1647, 1741
BssMI GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
BssT1I CCWWGG 2 cut(s) 950, 1437
Bst2UI CCWGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
Bst4CI ACNGT 8 cut(s) 424, 443, 779, 920, 1251, 1791, 1913, 1967
Bst6I CTCTTC 3 cut(s) 105, 1005, 2112
BstAFI CTTAAG 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 981
BstDEI CTNAG 8 cut(s) 120, 206, 539, 876, 1212, 1536, 2139, 2163
BstDSI CCRYGG 2 cut(s) 1437, 1647
BstENI CCTNNNNNAGG 2 cut(s) 96, 2019
BstF5I GGATG 9 cut(s) 259, 645, 754, 789, 946, 1112, 1222, 1227, 1447
BstFNI CGCG 1 cut(s) 1661
BstKTI GATC 8 cut(s) 37, 91, 628, 1032, 1412, 1431, 1768, 2033
BstMAI GTCTC 1 cut(s) 2088
BstMBI GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
BstNI CCWGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
BstNSI RCATGY 3 cut(s) 796, 1132, 1390
BstSCI CCNGG 5 cut(s) 1184, 1350, 1601, 1741, 1862
BstSFI CTRYAG 4 cut(s) 46, 778, 1463, 1574
BstUI CGCG 1 cut(s) 1661
BstV1I GCAGC 6 cut(s) 1610, 1631, 1771, 1876, 2129, 2225
BstV2I GAAGAC 2 cut(s) 131, 1155
BstXI CCANNNNNNTGG 2 cut(s) 488, 726
Bsu15I ATCGAT 1 cut(s) 1496
Bsu36I CCTNAGG 1 cut(s) 206
BsuI GTATCC 3 cut(s) 948, 1182, 1249
BsuRI GGCC 8 cut(s) 281, 339, 346, 363, 585, 1015, 1746, 2087
BsuTUI ATCGAT 1 cut(s) 1496
BtgI CCRYGG 2 cut(s) 1437, 1647
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 9 cut(s) 259, 645, 754, 789, 946, 1112, 1222, 1227, 1447
BtsI GCAGTG 2 cut(s) 640, 1460
BtsIMutI CAGTG 7 cut(s) 181, 420, 640, 916, 1256, 1460, 2175
CaiI CAGNNNCTG 1 cut(s) 1892
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 4 cut(s) 362, 572, 696, 830
ClaI ATCGAT 1 cut(s) 1496
CseI GACGC 1 cut(s) 899
CsiI ACCWGGT 1 cut(s) 1862
Csp6I GTAC 7 cut(s) 982, 990, 1738, 1792, 1828, 1860, 2042
CviQI GTAC 7 cut(s) 982, 990, 1738, 1792, 1828, 1860, 2042
DdeI CTNAG 8 cut(s) 120, 206, 539, 876, 1212, 1536, 2139, 2163
DpnI GATC 8 cut(s) 36, 90, 627, 1031, 1411, 1430, 1767, 2032
DpnII GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
DraI TTTAAA 3 cut(s) 223, 1171, 1476
EaeI YGGCCR 4 cut(s) 337, 583, 1013, 1744
Eam1104I CTCTTC 3 cut(s) 105, 1005, 2112
EarI CTCTTC 3 cut(s) 105, 1005, 2112
Eco130I CCWWGG 2 cut(s) 950, 1437
Eco24I GRGCYC 2 cut(s) 1045, 2245
Eco31I GGTCTC 1 cut(s) 2088
Eco47I GGWCC 3 cut(s) 572, 696, 830
Eco57I CTGAAG 3 cut(s) 114, 129, 1167
Eco81I CCTNAGG 1 cut(s) 206
EcoNI CCTNNNNNAGG 2 cut(s) 96, 2019
EcoO109I RGGNCCY 1 cut(s) 572
EcoRI GAATTC 1 cut(s) 1652
EcoRII CCWGG 5 cut(s) 1184, 1350, 1601, 1741, 1862
EcoT14I CCWWGG 2 cut(s) 950, 1437
EcoT22I ATGCAT 2 cut(s) 756, 1130
EcoT38I GRGCYC 2 cut(s) 1045, 2245
ErhI CCWWGG 2 cut(s) 950, 1437
FalI AAGNNNNNCTT 4 cut(s) 123, 155, 1266, 1298
FauI CCCGC 1 cut(s) 2107
FauNDI CATATG 2 cut(s) 986, 1855
FbaI TGATCA 3 cut(s) 625, 1029, 1428
Fnu4HI GCNGC 6 cut(s) 1599, 1620, 1785, 1890, 2118, 2214
FokI GGATG 9 cut(s) 266, 632, 741, 796, 933, 1119, 1229, 1234, 1454
FriOI GRGCYC 2 cut(s) 1045, 2245
Fsp4HI GCNGC 6 cut(s) 1599, 1620, 1785, 1890, 2118, 2214
FspBI CTAG 3 cut(s) 330, 609, 1323
GluI GCNGC 6 cut(s) 1599, 1620, 1785, 1890, 2118, 2214
GsuI CTGGAG 2 cut(s) 1635, 2199
HaeIII GGCC 8 cut(s) 281, 339, 346, 363, 585, 1015, 1746, 2087
HapII CCGG 3 cut(s) 360, 699, 1508
HgaI GACGC 1 cut(s) 899
HincII GTYRAC 2 cut(s) 1247, 1884
HindII GTYRAC 2 cut(s) 1247, 1884
HindIII AAGCTT 2 cut(s) 8, 1275
HinfI GANTC 3 cut(s) 619, 730, 1642
HpaI GTTAAC 1 cut(s) 1884
HpaII CCGG 3 cut(s) 360, 699, 1508
HphI GGTGA 5 cut(s) 191, 1172, 1385, 1532, 2165
Hpy166II GTNNAC 7 cut(s) 307, 400, 790, 1247, 1416, 1884, 2044
Hpy188I TCNGA 4 cut(s) 477, 1428, 1455, 2142
Hpy188III TCNNGA 9 cut(s) 92, 623, 699, 1033, 1046, 1342, 1432, 1954, 2018
Hpy8I GTNNAC 7 cut(s) 307, 400, 790, 1247, 1416, 1884, 2044
HpyAV CCTTC 9 cut(s) 63, 291, 386, 503, 563, 747, 895, 2015, 2286
HpyCH4III ACNGT 8 cut(s) 424, 443, 779, 920, 1251, 1791, 1913, 1967
HpyF3I CTNAG 8 cut(s) 120, 206, 539, 876, 1212, 1536, 2139, 2163
Kpn2I TCCGGA 1 cut(s) 698
KpnI GGTACC 1 cut(s) 1863
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Ksp22I TGATCA 3 cut(s) 625, 1029, 1428
KspAI GTTAAC 1 cut(s) 1884
Kzo9I GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
LmnI GCTCC 3 cut(s) 994, 1510, 1616
Lsp1109I GCAGC 6 cut(s) 1610, 1631, 1771, 1876, 2129, 2225
LweI GCATC 6 cut(s) 421, 763, 1186, 1218, 1491, 1540
MabI ACCWGGT 1 cut(s) 1862
MaeI CTAG 3 cut(s) 330, 609, 1323
MaeIII GTNAC 5 cut(s) 179, 458, 637, 1051, 1373
MalI GATC 8 cut(s) 36, 90, 627, 1031, 1411, 1430, 1767, 2032
MbiI CCGCTC 1 cut(s) 1540
MboI GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
MhlI GDGCHC 2 cut(s) 1045, 2245
MlsI TGGCCA 4 cut(s) 339, 585, 1015, 1746
MluNI TGGCCA 4 cut(s) 339, 585, 1015, 1746
Mox20I TGGCCA 4 cut(s) 339, 585, 1015, 1746
Mph1103I ATGCAT 2 cut(s) 756, 1130
MroI TCCGGA 1 cut(s) 698
MroNI GCCGGC 1 cut(s) 359
MscI TGGCCA 4 cut(s) 339, 585, 1015, 1746
MslI CAYNNNNRTG 6 cut(s) 551, 633, 797, 918, 1683, 2180
Msp20I TGGCCA 4 cut(s) 339, 585, 1015, 1746
MspA1I CMGCKG 2 cut(s) 1613, 1889
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 3 cut(s) 360, 699, 1508
MspR9I CCNGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
Mva1269I GAATGC 2 cut(s) 201, 1697
MvaI CCWGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
MvnI CGCG 1 cut(s) 1661
NaeI GCCGGC 1 cut(s) 361
NcoI CCATGG 1 cut(s) 1437
NdeI CATATG 2 cut(s) 986, 1855
NdeII GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
NgoMIV GCCGGC 1 cut(s) 359
NheI GCTAGC 1 cut(s) 329
NlaIV GGNNCC 5 cut(s) 996, 1533, 1608, 1861, 2268
NmuCI GTSAC 2 cut(s) 179, 1373
NsiI ATGCAT 2 cut(s) 756, 1130
NspI RCATGY 3 cut(s) 796, 1132, 1390
OliI CACNNNNGTG 1 cut(s) 2180
PaeI GCATGC 2 cut(s) 1132, 1390
PctI GAATGC 2 cut(s) 201, 1697
PdiI GCCGGC 1 cut(s) 361
PfeI GAWTC 3 cut(s) 619, 730, 1642
PflFI GACNNNGTC 1 cut(s) 1337
PkrI GCNGC 6 cut(s) 1600, 1621, 1786, 1891, 2119, 2215
PpuMI RGGWCCY 1 cut(s) 572
PshBI ATTAAT 1 cut(s) 1935
Psp5II RGGWCCY 1 cut(s) 572
Psp6I CCWGG 5 cut(s) 1184, 1350, 1601, 1741, 1862
PspGI CCWGG 5 cut(s) 1184, 1350, 1601, 1741, 1862
PspN4I GGNNCC 5 cut(s) 996, 1533, 1608, 1861, 2268
PspPI GGNCC 4 cut(s) 362, 572, 696, 830
PspPPI RGGWCCY 1 cut(s) 572
PstI CTGCAG 2 cut(s) 1467, 1578
PstNI CAGNNNCTG 1 cut(s) 1892
PsyI GACNNNGTC 1 cut(s) 1337
PvuII CAGCTG 2 cut(s) 1613, 1889
RsaI GTAC 7 cut(s) 983, 991, 1739, 1793, 1829, 1861, 2043
RsaNI GTAC 7 cut(s) 982, 990, 1738, 1792, 1828, 1860, 2042
RseI CAYNNNNRTG 6 cut(s) 551, 633, 797, 918, 1683, 2180
SatI GCNGC 6 cut(s) 1599, 1620, 1785, 1890, 2118, 2214
Sau3AI GATC 8 cut(s) 34, 88, 625, 1029, 1409, 1428, 1765, 2030
Sau96I GGNCC 4 cut(s) 362, 572, 696, 830
ScrFI CCNGG 5 cut(s) 1186, 1352, 1603, 1743, 1864
SduI GDGCHC 2 cut(s) 1045, 2245
SexAI ACCWGGT 1 cut(s) 1862
SfaNI GCATC 6 cut(s) 421, 763, 1186, 1218, 1491, 1540
SfcI CTRYAG 4 cut(s) 46, 778, 1463, 1574
SinI GGWCC 3 cut(s) 572, 696, 830
SmiMI CAYNNNNRTG 6 cut(s) 551, 633, 797, 918, 1683, 2180
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SphI GCATGC 2 cut(s) 1132, 1390
SsiI CCGC 6 cut(s) 157, 1393, 1540, 1661, 2100, 2257
SspMI CTAG 3 cut(s) 330, 609, 1323
StyD4I CCNGG 5 cut(s) 1184, 1350, 1601, 1741, 1862
StyI CCWWGG 2 cut(s) 950, 1437
TaaI ACNGT 8 cut(s) 424, 443, 779, 920, 1251, 1791, 1913, 1967
TaqI TCGA 4 cut(s) 674, 1047, 1113, 1496
TatI WGTACW 2 cut(s) 981, 2041
TfiI GAWTC 3 cut(s) 619, 730, 1642
TscAI CASTG 7 cut(s) 181, 427, 640, 923, 1256, 1467, 2182
TseFI GTSAC 2 cut(s) 179, 1373
TseI GCWGC 6 cut(s) 1598, 1619, 1784, 1889, 2117, 2213
Tsp45I GTSAC 2 cut(s) 179, 1373
TspGWI ACGGA 2 cut(s) 1007, 1664
TspRI CASTG 7 cut(s) 181, 427, 640, 923, 1256, 1467, 2182
Tth111I GACNNNGTC 1 cut(s) 1337
Vha464I CTTAAG 1 cut(s) 11
VpaK11BI GGWCC 3 cut(s) 572, 696, 830
VspI ATTAAT 1 cut(s) 1935
XagI CCTNNNNNAGG 2 cut(s) 96, 2019
XapI RAATTY 3 cut(s) 450, 1652, 2035
XceI RCATGY 3 cut(s) 796, 1132, 1390
XcmI CCANNNNNNNNNTGG 2 cut(s) 765, 1200
XspI CTAG 3 cut(s) 330, 609, 1323
Zsp2I ATGCAT 2 cut(s) 756, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.