RLG00000020360

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000173
Physical Location & Seq
Forward (+)
64216717 .. 64221812
5096 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020360

Sequence Viewer

Length: 2409 bp
ATGAACAAGGTGGGCTATAATTTATTGTCGGCCTCCCCATCCTTCCTATCTATAAAGTGGGCACCCCATGCATCTCAAACATCACAATTCACAATTCACAACCCATCAAACTCCTCTCTCTCTCTGTGTCTCTTCAGGACTCCTCTCTCACTCCATCAGCAACCGGTTTCAGATCGGCCGTACTATAGAGAAAGGCTACTTGTCACATGGGAGTTTGACCCCGATGCAGGCACACCGGAAGAGCGCGCTGAGGTTGAGGCCGCTCGTGAGAATTTCTACCAAAATCGCTTCAAGGTTGCTCCCAGCAGTGACCTCCTCTGGCGTTTTCAGATGCTAAGAGAGAGAAACTTCAAGCAAGAAATTCCTCCGGTGAGAATTGGGGAAGGTGAGGAAATCACATTTGATCAGGCCACAGCTGCATACAAGAGGGCTTCTTCTTTCTGGAATGCCTTGCAGTCACCCCATGGTCACTGGCCTGCTGAGAATGCTGGCCCAAACTTTTACTTTCCTCCCTTGGTCATGAGTGTATACATACCAGGATACCTCAATGTTGTTTTCCCTGCTGAGTACAAGAAGGAAATCTTGCGCTACACATACAACCATCAGAACGAAGATGGTGGCTGGGGTTTGCACATTGCAGGGCCTAGTATGATGTTCACCACATGTCTCAACTATTGTATGATGCGTATTCTCGGAGAAGGCCCTGAGGGTGGTCGAGACAACGCCTGTGCAAGGGCCCGGAAATGGATTCTTGACCGCGGTGGTGCACAATATTCTGCCTCATGGGGAAAGACCTGGATGGCGATACTTGGTGTTTATGATTGGGAAGGAAGCAACCCAATGCCCCCAGAGTTTTGGAGCTACCCTACTCTGCTTCCATTTCATCCTTCAAAAATGTTTTGCTACTGTAGGTTGACGTACCTACCCATGTCTTACTTCTATGCAACAAGATTTGTTGGCCCAATCACTCCTCTTGTTGAGCAATTGAGACAAGAAATCTACAATGAGCCTTACGATCAAATCAAGTGGAGTCAAGTGCGCCATCATTGTGCAACAGAAGATAACTACTATCCTCATGGAAGAGTACAGCGCTTTATGTGGGATAGTCTTTACAATGTTTGTGAGCCTCTTCTCAAAATCTGGCCCTTCAAAAAGATCAGAGACAATGCTGTTCAATTCACCATTGACCAAATTCATTACGAAGATGAGAACAGTCGCTACATTACCATTGGATGTGTTGAAAAGCCATTGATGATGCTTGCTTGTTGGGCTGAGGATCCTAGTGGAGAAGCTTTTAAGAAGCATCTTCCTAGAGTTACTGACTATATTTGGCTCGGTGAAGATGGTATCAAGATGCAGAGTTTTGGTAGTCAGTCATGGGATTGTGCTCTTGTGATTCAAGCATTGCTTTGTGGGAATCTCAACAATGAATTTGGACCTGTACTTAAGTTGGCACATGACTTCCTAAAGAAATCTCAGGTGAGGGTGAATACTTCCGGCGACTACTTGGCTCATTTCCGTCATATTTCTAAAGGATGCTGGACTTTCTCTGATCGGGATCATGGATGGCAAGTGTCTGACTGCACTGCAGAAGCATTGAGGTGCTGCTGTCTGTTTGCAATGATGTCACCAGAGCTTGTTGGTGAGCCAATGGAAGCTGAGTGTATGTATGATGCTGTCAATGTCATACTGTCACTACAGAGTCCAAATGGTGGTGTATCGGCCTGGGAGCCAACTGGAGCTCCTAAATGGTTGGAGTGGCTCAACCCCGTCGAATTTCTTGAGGACCTTGTCATTGAATACGAATACATCGAGTGTACTTCATCTTCAATCCAGGCCCTAATATTGTTTAGGAAGTTGTACCCTGGTCACAGAAGGAAGGAGATTAACAACTTCATTACAAGGGCTGCAGACTACATTGAAAGTATCCAATACCCCGATGGTTCATGGTATGGAAACTGGGGTATCTGCTTTGTCTATGGAACATGGTTCGCAATCAAAGGATTAGAGGCTGCTGGAAGAACATACAACAACTGTGAGGCTGTTCGTAAAGGTGTCGATTTCTTGCTCAATTCACAGAGGGAAGATGGTGGTTGGGGAGAGCACTACACCTCATGCACAAATAAGAAATACACAGCTCAGGACAGTACAAATCTGGTGCAAACTGCCCTTGGATTGATGGGTCTAATTCACGGTCGACAGGCTGAGAGAGATCCAACTCCTATCCACCGTGCCGCAAGGGTGTTGATGAATGGTCAATTGGATGACGGTGATTTCCCCCAACAGGAACTGATGGGAGTTTTCATGAGGAATGCCATGTTGCACTATGCAGCATATAGAAATATCTTCCCATTGTGGGCTCTCGGAGAGTACCGCACTCTGGTGCAATTGCCTACCAAAAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

803

Amino Acids

92.36

Weight (kDa)

6.03

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 187 - 439 2.3e-32 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 459 - 792 1.7e-48 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccB7I CCANNNNNTGG 1 cut(s) 1712
AccBSI CCGCTC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 528, 2197
AccII CGCG 2 cut(s) 246, 759
AciI CCGC 5 cut(s) 261, 757, 759, 2235, 2374
AclWI GGATC 4 cut(s) 1271, 1284, 1566, 2207
AcoI YGGCCR 1 cut(s) 176
AcsI RAATTY 5 cut(s) 271, 360, 1191, 1430, 1775
AcuI CTGAAG 1 cut(s) 118
AfaI GTAC 9 cut(s) 182, 569, 920, 1086, 1443, 1819, 1862, 2149, 2372
AfeI AGCGCT 1 cut(s) 1091
AfiI CCNNNNNNNGG 8 cut(s) 227, 710, 732, 744, 1712, 2284, 2356, 2380
AflII CTTAAG 1 cut(s) 1445
AflIII ACRYGT 1 cut(s) 662
AgeI ACCGGT 1 cut(s) 163
AjnI CCWGG 5 cut(s) 535, 794, 1724, 1833, 1864
AjuI GAANNNNNNNTTGG 2 cut(s) 2243, 2275
AleI CACNNNNGTG 1 cut(s) 2381
AluBI AGCT 7 cut(s) 416, 861, 1292, 1636, 1658, 1742, 2138
AluI AGCT 7 cut(s) 416, 861, 1292, 1636, 1658, 1742, 2138
Alw21I GWGCWC 4 cut(s) 769, 1390, 1744, 2106
Alw26I GTCTC 5 cut(s) 134, 671, 711, 982, 1155
Alw44I GTGCAC 1 cut(s) 765
AlwI GGATC 4 cut(s) 1271, 1284, 1566, 2207
AlwNI CAGNNNCTG 1 cut(s) 2290
Aor51HI AGCGCT 1 cut(s) 1091
ApaI GGGCCC 1 cut(s) 739
ApaLI GTGCAC 1 cut(s) 765
ApeKI GCWGC 5 cut(s) 416, 1605, 1907, 2012, 2330
ApoI RAATTY 5 cut(s) 271, 360, 1191, 1430, 1775
AsiGI ACCGGT 1 cut(s) 163
AspLEI GCGC 5 cut(s) 246, 248, 588, 1041, 1092
AsuC2I CCSGG 1 cut(s) 739
AvaII GGWCC 2 cut(s) 1436, 1786
AxyI CCTNAGG 1 cut(s) 705
BaeGI GKGCMC 3 cut(s) 64, 739, 769
BaeI ACNNNNGTAYC 2 cut(s) 1948, 1981
BamHI GGATCC 1 cut(s) 1276
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 3 cut(s) 739, 1744, 2362
BarI GAAGNNNNNNTAC 2 cut(s) 1810, 1842
BauI CACGAG 1 cut(s) 264
Bbv12I GWGCWC 4 cut(s) 769, 1390, 1744, 2106
BbvCI CCTCAGC 2 cut(s) 249, 1272
BbvI GCAGC 5 cut(s) 403, 1592, 1894, 1999, 2342
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 5 cut(s) 537, 796, 1726, 1835, 1866
BciVI GTATCC 2 cut(s) 533, 1937
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 5 cut(s) 134, 671, 711, 982, 1155
BfaI CTAG 3 cut(s) 645, 1281, 1311
BfmI CTRYAG 5 cut(s) 184, 907, 1587, 1697, 1908
BfoI RGCGCY 1 cut(s) 1093
BfrI CTTAAG 1 cut(s) 1445
BfuI GTATCC 2 cut(s) 533, 1937
BisI GCNGC 7 cut(s) 261, 417, 1606, 1908, 2013, 2235, 2331
BlsI GCNGC 7 cut(s) 262, 418, 1607, 1909, 2014, 2236, 2332
Bme1390I CCNGG 6 cut(s) 537, 739, 796, 1726, 1835, 1866
Bme18I GGWCC 2 cut(s) 1436, 1786
BmiI GGNNCC 4 cut(s) 63, 737, 1278, 1731
BmrFI CCNGG 6 cut(s) 537, 739, 796, 1726, 1835, 1866
BmrI ACTGGG 1 cut(s) 1969
BmsI GCATC 9 cut(s) 80, 214, 321, 672, 1245, 1312, 1344, 1526, 1663
BmuI ACTGGG 1 cut(s) 1969
BpmI CTGGAG 1 cut(s) 1758
Bpu10I CCTNAGC 3 cut(s) 249, 1272, 2139
BpuEI CTTGAG 1 cut(s) 1802
BpuMI CCSGG 1 cut(s) 739
BsaBI GATNNNNATC 1 cut(s) 1557
BsaJI CCNNGG 6 cut(s) 463, 513, 757, 1725, 1864, 2170
BsaWI WCCGGW 3 cut(s) 163, 235, 367
BsaXI ACNNNNNCTCC 2 cut(s) 1726, 1756
Bsc4I CCNNNNNNNGG 8 cut(s) 227, 710, 732, 744, 1712, 2284, 2356, 2380
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 3 cut(s) 476, 1741, 1964
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 3 cut(s) 633, 1403, 1626
Bse8I GATNNNNATC 1 cut(s) 1557
BseBI CCWGG 5 cut(s) 537, 796, 1726, 1835, 1866
BseDI CCNNGG 6 cut(s) 463, 513, 757, 1725, 1864, 2170
BseGI GGATG 7 cut(s) 38, 804, 883, 1238, 1541, 1571, 2269
BseJI GATNNNNATC 1 cut(s) 1557
BseLI CCNNNNNNNGG 8 cut(s) 227, 710, 732, 744, 1712, 2284, 2356, 2380
BseMI GCAATG 3 cut(s) 633, 1403, 1626
BseMII CTCAG 9 cut(s) 240, 471, 555, 696, 1263, 1490, 1650, 2153, 2196
BseNI ACTGG 3 cut(s) 476, 1741, 1964
BsePI GCGCGC 1 cut(s) 244
BseRI GAGGAG 4 cut(s) 103, 132, 305, 960
BseSI GKGCMC 3 cut(s) 64, 739, 769
BseX3I CGGCCG 1 cut(s) 176
BseXI GCAGC 5 cut(s) 403, 1592, 1894, 1999, 2342
BseYI CCCAGC 2 cut(s) 302, 621
BsgI GTGCAG 1 cut(s) 1567
Bsh1236I CGCG 2 cut(s) 246, 759
Bsh1285I CGRYCG 2 cut(s) 179, 2197
BshNI GGYRCC 1 cut(s) 61
BshTI ACCGGT 1 cut(s) 163
BsiEI CGRYCG 2 cut(s) 179, 2197
BsiHKAI GWGCWC 4 cut(s) 769, 1390, 1744, 2106
BsiSI CCGG 5 cut(s) 164, 236, 368, 739, 1497
BslI CCNNNNNNNGG 8 cut(s) 227, 710, 732, 744, 1712, 2284, 2356, 2380
BsmAI GTCTC 5 cut(s) 134, 671, 711, 982, 1155
BsmI GAATGC 3 cut(s) 451, 490, 2317
Bsp120I GGGCCC 1 cut(s) 735
Bsp1286I GDGCHC 7 cut(s) 64, 739, 769, 1390, 1744, 2106, 2362
Bsp143I GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
Bsp19I CCATGG 1 cut(s) 463
BspACI CCGC 5 cut(s) 261, 757, 759, 2235, 2374
BspCNI CTCAG 9 cut(s) 241, 472, 556, 697, 1264, 1489, 1651, 2152, 2197
BspFNI CGCG 2 cut(s) 246, 759
BspHI TCATGA 2 cut(s) 519, 2304
BspLI GGNNCC 4 cut(s) 63, 737, 1278, 1731
BspMAI CTGCAG 2 cut(s) 1591, 1912
BspPI GGATC 4 cut(s) 1271, 1284, 1566, 2207
BspQI GCTCTTC 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 61
BspTI CTTAAG 1 cut(s) 1445
BsrBI CCGCTC 1 cut(s) 263
BsrDI GCAATG 3 cut(s) 633, 1403, 1626
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 3 cut(s) 476, 1741, 1964
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 6 cut(s) 463, 513, 757, 1725, 1864, 2170
BssHII GCGCGC 1 cut(s) 244
BssMI GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
BssNAI GTATAC 1 cut(s) 529
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 3 cut(s) 463, 513, 2170
Bst1107I GTATAC 1 cut(s) 529
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 5 cut(s) 537, 796, 1726, 1835, 1866
Bst4CI ACNGT 8 cut(s) 908, 1214, 1692, 2036, 2147, 2195, 2231, 2270
Bst6I CTCTTC 4 cut(s) 137, 234, 1075, 1134
BstAFI CTTAAG 1 cut(s) 1445
BstAPI GCANNNNNTGC 1 cut(s) 68
BstC8I GCNNGC 5 cut(s) 229, 246, 477, 490, 1260
BstDSI CCRYGG 2 cut(s) 463, 757
BstENI CCTNNNNNAGG 1 cut(s) 730
BstF5I GGATG 7 cut(s) 38, 804, 883, 1238, 1541, 1571, 2269
BstFNI CGCG 2 cut(s) 246, 759
BstH2I RGCGCY 1 cut(s) 1093
BstHHI GCGC 5 cut(s) 246, 248, 588, 1041, 1092
BstKTI GATC 8 cut(s) 175, 406, 1018, 1158, 1279, 1555, 1561, 2215
BstMAI GTCTC 5 cut(s) 134, 671, 711, 982, 1155
BstMBI GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
BstMCI CGRYCG 2 cut(s) 179, 2197
BstMWI GCNNNNNNNGC 4 cut(s) 68, 416, 485, 1268
BstNI CCWGG 5 cut(s) 537, 796, 1726, 1835, 1866
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 6 cut(s) 535, 737, 794, 1724, 1833, 1864
BstSFI CTRYAG 5 cut(s) 184, 907, 1587, 1697, 1908
BstSLI GKGCMC 3 cut(s) 64, 739, 769
BstUI CGCG 2 cut(s) 246, 759
BstV1I GCAGC 5 cut(s) 403, 1592, 1894, 1999, 2342
BstX2I RGATCY 2 cut(s) 1276, 2212
BstXI CCANNNNNNTGG 1 cut(s) 855
BstYI RGATCY 2 cut(s) 1276, 2212
BstZ17I GTATAC 1 cut(s) 529
BstZI CGGCCG 1 cut(s) 176
Bsu36I CCTNAGG 1 cut(s) 705
BsuI GTATCC 2 cut(s) 533, 1937
BtgI CCRYGG 2 cut(s) 463, 757
BtsCI GGATG 7 cut(s) 38, 804, 883, 1238, 1541, 1571, 2269
BtsI GCAGTG 2 cut(s) 313, 1584
BtsIMutI CAGTG 3 cut(s) 313, 469, 1584
Cac8I GCNNGC 5 cut(s) 229, 246, 477, 490, 1260
CaiI CAGNNNCTG 1 cut(s) 2290
CciI TCATGA 2 cut(s) 519, 2304
CfoI GCGC 5 cut(s) 246, 248, 588, 1041, 1092
Cfr10I RCCGGY 1 cut(s) 163
Cfr42I CCGCGG 1 cut(s) 760
Csp6I GTAC 9 cut(s) 181, 568, 919, 1085, 1442, 1818, 1861, 2148, 2371
CspAI ACCGGT 1 cut(s) 163
CspCI CAANNNNNGTGG 2 cut(s) 1007, 1042
CviQI GTAC 9 cut(s) 181, 568, 919, 1085, 1442, 1818, 1861, 2148, 2371
DpnI GATC 8 cut(s) 174, 405, 1017, 1157, 1278, 1554, 1560, 2214
DpnII GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
EaeI YGGCCR 1 cut(s) 176
EagI CGGCCG 1 cut(s) 176
Eam1104I CTCTTC 4 cut(s) 137, 234, 1075, 1134
EarI CTCTTC 4 cut(s) 137, 234, 1075, 1134
Ecl136II GAGCTC 1 cut(s) 1742
EclXI CGGCCG 1 cut(s) 176
Eco130I CCWWGG 3 cut(s) 463, 513, 2170
Eco24I GRGCYC 3 cut(s) 739, 1744, 2362
Eco47I GGWCC 2 cut(s) 1436, 1786
Eco47III AGCGCT 1 cut(s) 1091
Eco52I CGGCCG 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 1742
Eco57I CTGAAG 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 1742
EcoNI CCTNNNNNAGG 1 cut(s) 730
EcoO109I RGGNCCY 5 cut(s) 641, 701, 735, 1786, 1837
EcoRII CCWGG 5 cut(s) 535, 794, 1724, 1833, 1864
EcoT14I CCWWGG 3 cut(s) 463, 513, 2170
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 3 cut(s) 739, 1744, 2362
ErhI CCWWGG 3 cut(s) 463, 513, 2170
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 1392, 1424
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 2 cut(s) 528, 2197
Fnu4HI GCNGC 7 cut(s) 261, 417, 1606, 1908, 2013, 2235, 2331
FokI GGATG 7 cut(s) 25, 811, 870, 1245, 1548, 1578, 2276
FriOI GRGCYC 3 cut(s) 739, 1744, 2362
Fsp4HI GCNGC 7 cut(s) 261, 417, 1606, 1908, 2013, 2235, 2331
FspBI CTAG 3 cut(s) 645, 1281, 1311
GlaI GCGC 5 cut(s) 245, 247, 587, 1040, 1091
GluI GCNGC 7 cut(s) 261, 417, 1606, 1908, 2013, 2235, 2331
GsaI CCCAGC 2 cut(s) 306, 625
GsuI CTGGAG 1 cut(s) 1758
HaeII RGCGCY 1 cut(s) 1093
HapII CCGG 5 cut(s) 164, 236, 368, 739, 1497
HhaI GCGC 5 cut(s) 246, 248, 588, 1041, 1092
Hin6I GCGC 5 cut(s) 244, 246, 586, 1039, 1090
HinP1I GCGC 5 cut(s) 244, 246, 586, 1039, 1090
HincII GTYRAC 2 cut(s) 915, 2198
HindII GTYRAC 2 cut(s) 915, 2198
HindIII AAGCTT 1 cut(s) 1290
HinfI GANTC 6 cut(s) 139, 748, 1030, 1396, 1417, 1702
HpaII CCGG 5 cut(s) 164, 236, 368, 739, 1497
Hpy166II GTNNAC 6 cut(s) 529, 657, 767, 915, 1818, 2198
Hpy188I TCNGA 8 cut(s) 172, 330, 606, 695, 1160, 1552, 1579, 2366
Hpy8I GTNNAC 6 cut(s) 529, 657, 767, 915, 1818, 2198
Hpy99I CGWCG 1 cut(s) 1775
HpyAV CCTTC 9 cut(s) 52, 377, 568, 692, 821, 897, 1156, 1869, 1873
HpyCH4III ACNGT 8 cut(s) 908, 1214, 1692, 2036, 2147, 2195, 2231, 2270
HpyCH4IV ACGT 1 cut(s) 917
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 416, 485, 1268
HpySE526I ACGT 1 cut(s) 917
HspAI GCGC 5 cut(s) 244, 246, 586, 1039, 1090
Ksp22I TGATCA 1 cut(s) 403
KspI CCGCGG 1 cut(s) 760
Kzo9I GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 5 cut(s) 304, 858, 1729, 1739, 1747
Lsp1109I GCAGC 5 cut(s) 403, 1592, 1894, 1999, 2342
LweI GCATC 9 cut(s) 80, 214, 321, 672, 1245, 1312, 1344, 1526, 1663
MaeI CTAG 3 cut(s) 645, 1281, 1311
MaeII ACGT 1 cut(s) 917
MaeIII GTNAC 8 cut(s) 202, 308, 456, 467, 1315, 1626, 1692, 1868
MalI GATC 8 cut(s) 174, 405, 1017, 1157, 1278, 1554, 1560, 2214
MbiI CCGCTC 1 cut(s) 263
MboI GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
MfeI CAATTG 3 cut(s) 983, 2258, 2387
MflI RGATCY 2 cut(s) 1276, 2212
MhlI GDGCHC 7 cut(s) 64, 739, 769, 1390, 1744, 2106, 2362
MlyI GAGTC 3 cut(s) 133, 1039, 1711
MmeI TCCRAC 2 cut(s) 1734, 2240
Mph1103I ATGCAT 1 cut(s) 73
MseI TTAA 3 cut(s) 1296, 1446, 1887
MslI CAYNNNNRTG 3 cut(s) 1047, 1600, 2381
MspA1I CMGCKG 2 cut(s) 416, 759
MspCI CTTAAG 1 cut(s) 1445
MspI CCGG 5 cut(s) 164, 236, 368, 739, 1497
MspR9I CCNGG 6 cut(s) 537, 739, 796, 1726, 1835, 1866
MunI CAATTG 3 cut(s) 983, 2258, 2387
Mva1269I GAATGC 3 cut(s) 451, 490, 2317
MvaI CCWGG 5 cut(s) 537, 796, 1726, 1835, 1866
MvnI CGCG 2 cut(s) 246, 759
MwoI GCNNNNNNNGC 4 cut(s) 68, 416, 485, 1268
NciI CCSGG 1 cut(s) 739
NcoI CCATGG 1 cut(s) 463
NdeII GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
NlaIV GGNNCC 4 cut(s) 63, 737, 1278, 1731
NmuCI GTSAC 7 cut(s) 202, 308, 456, 467, 1626, 1692, 1868
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 666
OliI CACNNNNGTG 1 cut(s) 2381
PagI TCATGA 2 cut(s) 519, 2304
PauI GCGCGC 1 cut(s) 244
PciI ACATGT 1 cut(s) 662
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 1809
PctI GAATGC 3 cut(s) 451, 490, 2317
PfeI GAWTC 3 cut(s) 748, 1396, 1417
PflFI GACNNNGTC 1 cut(s) 1790
PflMI CCANNNNNTGG 1 cut(s) 1712
PinAI ACCGGT 1 cut(s) 163
PkrI GCNGC 7 cut(s) 262, 418, 1607, 1909, 2014, 2236, 2332
PleI GAGTC 3 cut(s) 133, 1038, 1710
PpsI GAGTC 3 cut(s) 133, 1038, 1710
PpuMI RGGWCCY 1 cut(s) 1786
PscI ACATGT 1 cut(s) 662
Psp124BI GAGCTC 1 cut(s) 1744
Psp5II RGGWCCY 1 cut(s) 1786
Psp6I CCWGG 5 cut(s) 535, 794, 1724, 1833, 1864
PspFI CCCAGC 2 cut(s) 302, 621
PspGI CCWGG 5 cut(s) 535, 794, 1724, 1833, 1864
PspN4I GGNNCC 4 cut(s) 63, 737, 1278, 1731
PspOMI GGGCCC 1 cut(s) 735
PspPPI RGGWCCY 1 cut(s) 1786
PsrI GAACNNNNNNTAC 2 cut(s) 1202, 1234
PstI CTGCAG 2 cut(s) 1591, 1912
PstNI CAGNNNCTG 1 cut(s) 2290
PsuI RGATCY 2 cut(s) 1276, 2212
PsyI GACNNNGTC 1 cut(s) 1790
PteI GCGCGC 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 9 cut(s) 182, 569, 920, 1086, 1443, 1819, 1862, 2149, 2372
RsaNI GTAC 9 cut(s) 181, 568, 919, 1085, 1442, 1818, 1861, 2148, 2371
RseI CAYNNNNRTG 3 cut(s) 1047, 1600, 2381
SacI GAGCTC 1 cut(s) 1744
SacII CCGCGG 1 cut(s) 760
SalI GTCGAC 1 cut(s) 2196
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 3 cut(s) 1296, 1446, 1887
SatI GCNGC 7 cut(s) 261, 417, 1606, 1908, 2013, 2235, 2331
Sau3AI GATC 8 cut(s) 172, 403, 1015, 1155, 1276, 1552, 1558, 2212
SchI GAGTC 3 cut(s) 133, 1039, 1711
ScrFI CCNGG 6 cut(s) 537, 739, 796, 1726, 1835, 1866
SduI GDGCHC 7 cut(s) 64, 739, 769, 1390, 1744, 2106, 2362
SfaNI GCATC 9 cut(s) 80, 214, 321, 672, 1245, 1312, 1344, 1526, 1663
SfcI CTRYAG 5 cut(s) 184, 907, 1587, 1697, 1908
Sfr303I CCGCGG 1 cut(s) 760
SgrBI CCGCGG 1 cut(s) 760
SinI GGWCC 2 cut(s) 1436, 1786
SmiMI CAYNNNNRTG 3 cut(s) 1047, 1600, 2381
SmlI CTYRAG 2 cut(s) 1445, 1781
SmoI CTYRAG 2 cut(s) 1445, 1781
SsiI CCGC 5 cut(s) 261, 757, 759, 2235, 2374
SspI AATATT 2 cut(s) 773, 1845
SspMI CTAG 3 cut(s) 645, 1281, 1311
SstI GAGCTC 1 cut(s) 1744
StyD4I CCNGG 6 cut(s) 535, 737, 794, 1724, 1833, 1864
StyI CCWWGG 3 cut(s) 463, 513, 2170
TaaI ACNGT 8 cut(s) 908, 1214, 1692, 2036, 2147, 2195, 2231, 2270
TaiI ACGT 1 cut(s) 920
TaqI TCGA 5 cut(s) 715, 1773, 1812, 2058, 2197
TatI WGTACW 5 cut(s) 567, 1084, 1441, 1817, 2147
TauI GCSGC 2 cut(s) 263, 2237
TfiI GAWTC 3 cut(s) 748, 1396, 1417
Tru1I TTAA 3 cut(s) 1296, 1446, 1887
Tru9I TTAA 3 cut(s) 1296, 1446, 1887
TscAI CASTG 3 cut(s) 313, 476, 1591
TseFI GTSAC 7 cut(s) 202, 308, 456, 467, 1626, 1692, 1868
TseI GCWGC 5 cut(s) 416, 1605, 1907, 2012, 2330
Tsp45I GTSAC 7 cut(s) 202, 308, 456, 467, 1626, 1692, 1868
TspDTI ATGAA 9 cut(s) 17, 872, 1184, 1443, 1812, 1885, 1935, 2264, 2293
TspGWI ACGGA 1 cut(s) 1508
TspRI CASTG 3 cut(s) 313, 476, 1591
Tth111I GACNNNGTC 1 cut(s) 1790
Van91I CCANNNNNTGG 1 cut(s) 1712
Vha464I CTTAAG 1 cut(s) 1445
VneI GTGCAC 1 cut(s) 765
VpaK11BI GGWCC 2 cut(s) 1436, 1786
XagI CCTNNNNNAGG 1 cut(s) 730
XapI RAATTY 5 cut(s) 271, 360, 1191, 1430, 1775
XceI RCATGY 1 cut(s) 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 1937
XmiI GTMKAC 2 cut(s) 528, 2197
XspI CTAG 3 cut(s) 645, 1281, 1311
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.