Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000174

Physical Location & Seq

Genomic Coordinates Reverse (-)

31278267 .. 31281368

Gene Structure 3102 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1240600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGTCACTGAGGTCTCGAAAACCTGCTGGACAACCAGCCATCGTCGATCAAGAAGATGTTCCAATCCCGAAACCCTACAAGCGGAGGTGGCGGTTGGCACTCACAGCCATATACTTCACCAGGGCTCTTGCTTCACTTGCCAAGAAACTACTACACGGTAACAATTCCCAGTTGCTACGCTCCCGCTCGTATGTAGCCATAGACGTGCCCAGGAATCAAGACGATCACGACAATGCACGTGACAAAAAGCACCTTCCTTTTCTCAACATTGATCAGAAGACGCTTGCTGACATGGTGAAGGAAAAAAACCTTGAGCTTCTTAGTCAATTTGGAGGGGTTCGAGAATTGGCTGCAGTTCTTGAGACTGACGTGAAAAGCGGCGTTAGAGGCGCTGAACCTGATCTGATGCATAGAAAGAATGTTTTCGGCACCAATGCGTACCAGAAACCGCCTGCGAAAGGGTTGCTGAGTTTCGTGCTTGAAGCTATCAAGGACACCACAATTATAATACTTTTGGTATGTGCATTACTTTCTCTAGGGTTTGGTATTAAACAGCATGGCTTGAAAGATGGGTGGTATGACGGTGGCAGCATTATCTTTGCTATATTTTTGGTTGTTATTGTGTCTGCACTTTCCAACTTTAAGCAGTCAAGACAATTCGAAAAGCTTTCTTCAAAGAGTGATGACATTCGTGTGGAAGTTGTGAGGGATGGACAGCGCCGTCCTACATCAATCTTTGATGTTGTGGTTGGTGATCTTGTGTGTTTGAAGATCGGTGATCAGGTTCCGACGGATGGTTTGTTCTTAGAAGGGCATTCGTTGAAGGTGGATGAGTCTAGTATGACCGGAGAAAGTGATCACATTGAGATCAATGATGGAAGCAACCCTTTTCTTCTATCCGGTACAAAGGTGACAGATGGGTTTGGCTTGATGATTGTGACTTCTGTGGGCATGAACACGGCGTGGGGAGAGATGATGAGCTCGATCAGCCGCTCTTTGGATGAGCAAACACCTTTGCAAGTGCGCCTCAACAAGCTGACTTCGTACATTGGGAAGGTTGGTTTGACAGTGGCTCTCCTTGTTCTTGTGGTTTCTCTAATTCGTTATTTCACAGGAAACACCGTAGATGTCAATGGAAATAGGGAATTCGGAGGCAGAAAGATGAAGTTTGATGATGTGATGAATGGTGTTGTGGGGATTGTAGCTGCAGCAATTACCATTGTTGTGGTGGCGATTCCCGAAGGCCTGCCTCTGGCTGTTACTTTGACCTTGGCTTATTCAATGAAGAAAATGATGAATGACAATGCTTTGGTACGGAAGCTCTCTGCTTGCGAGACAATGGGATCGGCAACAACAATCTGCACAGACAAAACGGGTACTCTTACTTTGAATGAAATGAACGTTTCAGAGTTCTGGCTTGGTACACAACCGATGACAGAAGAGAACATCACCGTGATAGCGCCTAATGTCCTACATTTACTCCGAGAGGCTGTTGGTCTAAACACAACTGGAAGTATTTGTAAACCAAATTCTTCATCCCCTGCTGAGATTTCGGGTAGCCCTACTGAAAAAGCAATACTTTCATGGGCCTTGTTTGATTTGGGTATGAACATTGATGAAGTGAAACAAGGCTGTGAGATAATTCATGTAGAAACCTTCAATTCGCAGAAAAAGAGAAGCGGTGTTTTGATTAGGAGGAACAACGAAAAGAGTACTCAAACGCATTGGAAAGGAGCTGCTGAGATGATACTTACAATGTGTGCAAACTATTATGATAAAACTGGGGCGCTAAGAGCCATGAATGACGAAGAAAGAAAGCGTGTTGGATCAGCCATTCAGAACATGGCATCCAAAAGCCTCCGGTGCATTGCCTTCGCGCATAGAATGCTTGAAGACGAGAACGGTCAGGTTCCTGAGAAGCTTGAAGAGGGTGGAATGACACTTTTAGGTCTTGTGGGTATAAAGGATCCGTGTAGACCGGGAGTCAGAACAGCAGTAGACGGTTGCAGAGCTGCAGGAGTGAACATTAAGATGATAACTGGCGACAATCTGCATACTGCAAGAGCTATTGCGGTTGAGTGTGGGATACTCAAAACAGATGAAGATTTGGAGACTGAGGCTGTAGTCGAAGGGGTACAATTCAGAGGTTACTCACCTGAAGAGAGGATGGAAAGGATCGACAAGATACGCGTGATGGCAAGATCCTCACCATTTGACAAGCTTCTGATGGTGCAATGCTTGAAGCAGAAAGGCCATGTGGTTGCTGTCACAGGAGATGGTACGAATGATGCACCGGCGCTAAAAGAAGCAGACATTGGGCTTTCAATGGGGATCCAAGGCACCGAAGTTGCAAAGGAGAGCTCGGATATCGTCATCTTAGATGACAACTTCGCATCTGTTGTGACAGTTTTGAGGTGGGGAAGGTGTGTGTACAACAATATTCAAAAGTTCCTCCAGTTCCAACTCACTGTCAATGTCGCTGCGCTTGTTATCAACATTGTTGCTGCTGTTACTTCTGGTAAAGTTCCACTAACTGCAGTGCAGATGCTATGGGTGAATCTCATCATGGACACCTTGGGGGCTTTAGCTTTGGCCACGGAAGAACCGACAGATGAACTTATGGAAAAGAAGCCCGTGGGACGAACGGAACCACTCATTATTAGAATCATGTGGAGAAACCTCATTGCTCAGGCCTTGTATCAAATCACAATCTTGTTAACCCTACAGTTTAAGGGAAGATCCATCTTCGGCGTGGATGAAAAGGTCAAGAACACCCTTATCTTCAACACATTTGTTCTCTGCCAAATCTTCAACGAGTTCAATGCAAGAAAACTTGAAAAGAAGAACATATTCAAGGGGTTACTCAAGAGCAAGCTGTTTCTGGCAATAGTCGGTAGTACCATAGTTCTTCAACTGCTGATGGTGGAATTTCTGAAGAAGTTTGCCAGCACTGAGAGGCTGGACTGGGGACAATGGGGTGCTTGCGTCGGAATCGCAGCGTTCTCTTGGCCGATTGGCTGGCTTGTTAAGTGCATTCCAGTTTCAGGATGGCAGGTTCCAAGGCTAAGAGCTCCTGCATTCTCAAACACAGAGAAATATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1034

Amino Acids

113.72

Weight (kDa)

8.55

Isoelectric Point (pI)

35.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	111 - 178	3.5e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	230 - 329	7.1e-20	P-type ATPase actuator domain
Hydrolase	PF00702	452 - 771	1.5e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	491 - 660	2.9e-30	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	842 - 1011	2.3e-46	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22910
fragaria_vesca	FvH4_4g01640 FvH4_4g01670 FvH4_4g01690 FvH4_4g01690 FvH4_4g01692 FvH4_4g06940
malus_domestica	MD04G1223800.v1.1 MD12G1240600.v1.1 MD13G1201600.v1.1 MD15G1425600.v1.1 MD15G1425900.v1.1 MD15G1426000.v1.1 MD15G1426200.v1.1 MD15G1429900.v1.1 MD16G1186200.v1.1 MD16G1201500.v1.1 MD16G1201800.v1.1 MD16G1245900.v1.1
prunus_persica	Prupe.1G023100_v2.0.a1 Prupe.1G023200_v2.0.a1 Prupe.1G023300_v2.0.a1 Prupe.1G023400_v2.0.a1 Prupe.2G086400_v2.0.a1
pyrus_communis	pycom03g20400 pycom04g03220 pycom04g03230 pycom04g19720 pycom13g17500 pycom14g13160 pycom15g37720
rosa_chinensis	RchiOBHm_Chr4g0385871 RchiOBHm_Chr4g0388251 RchiOBHm_Chr4g0388261 RchiOBHm_Chr4g0388291 RchiOBHm_Chr4g0388301 RchiOBHm_Chr4g0388321 RchiOBHm_Chr4g0388331 RchiOBHm_Chr4g0388341 RchiOBHm_Chr4g0388491 RchiOBHm_Chr4g0388501 RchiOBHm_Chr4g0388521 RchiOBHm_Chr4g0388541 RchiOBHm_Chr4g0388581 RchiOBHm_Chr4g0388591 RchiOBHm_Chr5g0038901 RchiOBHm_Chr5g0039001 RchiOBHm_Chr6g0264011
rosa_laevigata	RLG00000003378 RLG00000003379 RLG00000010036 RLG00000010037 RLG00000010039 RLG00000010041 RLG00000010042 RLG00000010043 RLG00000010049 RLG00000010050 RLG00000010051 RLG00000010052 RLG00000010054 RLG00000010055 RLG00000010062 RLG00000010064 RLG00000010065 RLG00000010066 RLG00000010067 RLG00000010069 RLG00000010070 RLG00000010071 RLG00000014295 RLG00000033880
rosa_multiflora	Rmu_co8110336.1_g000001 Rmu_sc0000533.1_g000070 Rmu_sc0000533.1_g000082 Rmu_sc0000753.1_g000013 Rmu_sc0000753.1_g000024 Rmu_sc0000753.1_g000027 Rmu_sc0000753.1_g000029 Rmu_sc0000753.1_g000030 Rmu_sc0000753.1_g000037 Rmu_sc0000753.1_g000048 Rmu_sc0001241.1_g000003 Rmu_sc0001241.1_g000020 Rmu_sc0001241.1_g000034 Rmu_sc0001241.1_g000045 Rmu_sc0001949.1_g000015 Rmu_sc0001949.1_g000020 Rmu_sc0001949.1_g000023 Rmu_sc0001949.1_g000028 Rmu_sc0002357.1_g000005 Rmu_sc0002357.1_g000017 Rmu_sc0002357.1_g000032 Rmu_sc0002357.1_g000035 Rmu_sc0002357.1_g000043 Rmu_sc0002694.1_g000002 Rmu_sc0004459.1_g000018 Rmu_sc0004773.1_g000011 Rmu_sc0006339.1_g000009 Rmu_sc0008728.1_g000008 Rmu_sc0012275.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042120 Rroxscaffold_2G00113240 Rroxscaffold_2G00131810 Rroxscaffold_4G00314870 Rroxscaffold_5G00334730 Rroxscaffold_5G00334750 Rroxscaffold_5G00334770 Rroxscaffold_5G00334790 Rroxscaffold_5G00334850 Rroxscaffold_5G00334970 Rroxscaffold_5G00335010 Rroxscaffold_5G00335030 Rroxscaffold_5G00335040
rosa_rugosa	Rorug03G0301800 Rorug03G0316600 Rorug03G0316600 Rorug03G0316700 Rorug03G0316700 Rorug03G0316800.1 Rorug03G0317300 Rorug03G0317400 Rorug03G0318000 Rorug03G0318500 Rorug03G0318600 Rorug03G0318700 Rorug03G0319000 Rorug03G0319000 Rorug03G0319100 Rorug05G0175000 Rorug05G0175500
rosa_samantha	Rh4CG007500 Rh4CG022300 Rh4CG022400 Rh4CG022600 Rh4CG022700 Rh4CG023500 Rh4CG023700 Rh4CG023900 Rh4CG024000 Rh4CG024600 Rh4CG025200 Rh4CG025300 Rh4CG025700 Rh4CG025800 Rh4CG025900 Rh4CG026000 Rh4CG026100 Rh4CG026200 Rh5BG266500 Rh6AG128500 Rh6DG110800
rosa_wichuraiana	Rw0G009370 Rw4G000360 Rw4G001430 Rw4G001440 Rw4G001460 Rw4G001470 Rw4G001490 Rw4G001500 Rw4G001520 Rw4G001620 Rw4G001630 Rw4G001650 Rw4G001670 Rw5G024450 Rw5G024490 Rw6G011090 Rw6G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	506
AasI	GACNNNNNNGTC	1 cut(s)	720
Acc36I	ACCTGC	2 cut(s)	31, 3043
AccB1I	GGYRCC	2 cut(s)	428, 2339
AccBSI	CCGCTC	2 cut(s)	186, 993
AccI	GTMKAC	2 cut(s)	1975, 1998
AccII	CGCG	2 cut(s)	1877, 2190
AciI	CCGC	8 cut(s)	82, 91, 184, 378, 449, 991, 1680, 2072
AclI	AACGTT	1 cut(s)	1401
AclWI	GGATC	9 cut(s)	1351, 1834, 1961, 1974, 2183, 2196, 2326, 2339, 2733
AcoI	YGGCCR	2 cut(s)	2592, 3008
AcsI	RAATTY	3 cut(s)	1145, 1528, 2927
AcuI	CTGAAG	2 cut(s)	2178, 2954
AcvI	CACGTG	1 cut(s)	239
AdeI	CACNNNGTG	1 cut(s)	963
AfiI	CCNNNNNNNGG	6 cut(s)	81, 458, 794, 997, 1252, 3044
AflIII	ACRYGT	1 cut(s)	2188
AhdI	GACNNNNNGTC	1 cut(s)	1982
AjiI	CACGTC	2 cut(s)	205, 370
AjnI	CCWGG	2 cut(s)	119, 209
AjuI	GAANNNNNNNTTGG	2 cut(s)	2298, 2330
AloI	GAACNNNNNNTCC	6 cut(s)	785, 817, 1832, 1864, 3039, 3071
Alw21I	GWGCWC	3 cut(s)	983, 2363, 3073
Alw26I	GTCTC	4 cut(s)	18, 356, 1328, 2105
AlwI	GGATC	9 cut(s)	1351, 1834, 1961, 1974, 2183, 2196, 2326, 2339, 2733
AoxI	GGCC	6 cut(s)	1243, 1587, 2251, 2592, 2691, 3008
ApeKI	GCWGC	9 cut(s)	350, 588, 1205, 1208, 1736, 2012, 2480, 2504, 2996
ApoI	RAATTY	3 cut(s)	1145, 1528, 2927
ArsI	GACNNNNNNTTYG	2 cut(s)	781, 813
Asp700I	GAANNNNTTC	3 cut(s)	57, 422, 2849
AspLEI	GCGC	8 cut(s)	392, 720, 1026, 1462, 1789, 1879, 2299, 2485
AspS9I	GGNCC	1 cut(s)	1587
AsuC2I	CCSGG	1 cut(s)	1980
AsuHPI	GGTGA	9 cut(s)	109, 307, 764, 788, 922, 1441, 2145, 2199, 2566
AsuII	TTCGAA	1 cut(s)	660
BaeGI	GKGCMC	1 cut(s)	210
BaeI	ACNNNNGTAYC	2 cut(s)	894, 927
BalI	TGGCCA	1 cut(s)	2594
BamHI	GGATCC	2 cut(s)	1966, 2331
BanI	GGYRCC	2 cut(s)	428, 2339
BanII	GRGCYC	4 cut(s)	127, 983, 2363, 3073
BbrPI	CACGTG	1 cut(s)	239
BbsI	GAAGAC	2 cut(s)	284, 1899
Bbv12I	GWGCWC	3 cut(s)	983, 2363, 3073
BbvI	GCAGC	9 cut(s)	337, 600, 1192, 1220, 1723, 1999, 2467, 2491, 3008
BceAI	ACGGC	2 cut(s)	705, 975
BcgI	CGANNNNNNTGC	6 cut(s)	445, 479, 1855, 1889, 2804, 2838
BciT130I	CCWGG	2 cut(s)	121, 211
BciVI	GTATCC	1 cut(s)	2079
BclI	TGATCA	3 cut(s)	271, 778, 856
BcnI	CCSGG	1 cut(s)	1980
BcoDI	GTCTC	4 cut(s)	18, 356, 1328, 2105
BfaI	CTAG	2 cut(s)	536, 837
BfmI	CTRYAG	6 cut(s)	351, 1206, 2013, 2121, 2535, 2723
BfoI	RGCGCY	5 cut(s)	393, 721, 1463, 1790, 2300
BfuAI	ACCTGC	2 cut(s)	31, 3043
BfuI	GTATCC	1 cut(s)	2079
BmcAI	AGTACT	1 cut(s)	1714
Bme1390I	CCNGG	3 cut(s)	121, 211, 1980
BmeRI	GACNNNNNGTC	1 cut(s)	1982
BmgBI	CACGTC	2 cut(s)	205, 370
BmgT120I	GGNCC	1 cut(s)	1587
BmiI	GGNNCC	8 cut(s)	430, 786, 1911, 1968, 2333, 2341, 2649, 3057
BmrFI	CCNGG	3 cut(s)	121, 211, 1980
BmrI	ACTGGG	3 cut(s)	163, 1791, 2974
BmsI	GCATC	5 cut(s)	396, 1856, 2278, 2402, 2535
BmuI	ACTGGG	3 cut(s)	163, 1791, 2974
BpiI	GAAGAC	2 cut(s)	284, 1899
BpmI	CTGGAG	1 cut(s)	2438
Bpu10I	CCTNAGC	1 cut(s)	2688
Bpu14I	TTCGAA	1 cut(s)	660
BpuEI	CTTGAG	3 cut(s)	332, 380, 2849
BpuMI	CCSGG	1 cut(s)	1980
BsaAI	YACGTR	1 cut(s)	239
BsaI	GGTCTC	1 cut(s)	18
BsaJI	CCNNGG	8 cut(s)	120, 209, 1269, 2335, 2574, 2595, 2634, 3059
BsaWI	WCCGGW	3 cut(s)	845, 899, 1860
BsaXI	ACNNNNNCTCC	2 cut(s)	1464, 1494
Bsc4I	CCNNNNNNNGG	6 cut(s)	81, 458, 794, 997, 1252, 3044
Bse118I	RCCGGY	1 cut(s)	2293
Bse1I	ACTGG	7 cut(s)	169, 1513, 1786, 2044, 2455, 2969, 3038
Bse3DI	GCAATG	3 cut(s)	1866, 2240, 2682
BseBI	CCWGG	2 cut(s)	121, 211
BseDI	CCNNGG	8 cut(s)	120, 209, 1269, 2335, 2574, 2595, 2634, 3059
BseGI	GGATG	9 cut(s)	715, 799, 835, 1006, 1535, 1847, 2172, 2761, 3053
BseLI	CCNNNNNNNGG	6 cut(s)	81, 458, 794, 997, 1252, 3044
BseMI	GCAATG	3 cut(s)	1866, 2240, 2682
BseMII	CTCAG	7 cut(s)	458, 1536, 1731, 1905, 2106, 2702, 2943
BseNI	ACTGG	7 cut(s)	169, 1513, 1786, 2044, 2455, 2969, 3038
BseSI	GKGCMC	1 cut(s)	210
BseXI	GCAGC	9 cut(s)	337, 600, 1192, 1220, 1723, 1999, 2467, 2491, 3008
BsgI	GTGCAG	3 cut(s)	612, 1345, 2561
Bsh1236I	CGCG	2 cut(s)	1877, 2190
BshFI	GGCC	6 cut(s)	1245, 1589, 2253, 2594, 2693, 3010
BshNI	GGYRCC	2 cut(s)	428, 2339
BsiHKAI	GWGCWC	3 cut(s)	983, 2363, 3073
BsiSI	CCGG	5 cut(s)	846, 900, 1861, 1979, 2294
BslFI	GGGAC	2 cut(s)	2652, 2982
BslI	CCNNNNNNNGG	6 cut(s)	81, 458, 794, 997, 1252, 3044
BsmAI	GTCTC	4 cut(s)	18, 356, 1328, 2105
BsmFI	GGGAC	2 cut(s)	2652, 2982
BsmI	GAATGC	4 cut(s)	814, 1890, 3033, 3077
BsnI	GGCC	6 cut(s)	1245, 1589, 2253, 2594, 2693, 3010
Bso31I	GGTCTC	1 cut(s)	18
Bsp119I	TTCGAA	1 cut(s)	660
Bsp1286I	GDGCHC	5 cut(s)	127, 210, 983, 2363, 3073
Bsp1407I	TGTACA	1 cut(s)	2430
BspACI	CCGC	8 cut(s)	82, 91, 184, 378, 449, 991, 1680, 2072
BspANI	GGCC	6 cut(s)	1245, 1589, 2253, 2594, 2693, 3010
BspCNI	CTCAG	7 cut(s)	459, 1537, 1732, 1906, 2107, 2701, 2944
BspFNI	CGCG	2 cut(s)	1877, 2190
BspLI	GGNNCC	8 cut(s)	430, 786, 1911, 1968, 2333, 2341, 2649, 3057
BspMAI	CTGCAG	4 cut(s)	355, 1210, 2017, 2539
BspMI	ACCTGC	2 cut(s)	31, 3043
BspPI	GGATC	9 cut(s)	1351, 1834, 1961, 1974, 2183, 2196, 2326, 2339, 2733
BspT104I	TTCGAA	1 cut(s)	660
BspT107I	GGYRCC	2 cut(s)	428, 2339
BspTNI	GGTCTC	1 cut(s)	18
BsrBI	CCGCTC	2 cut(s)	186, 993
BsrDI	GCAATG	3 cut(s)	1866, 2240, 2682
BsrFI	RCCGGY	1 cut(s)	2293
BsrGI	TGTACA	1 cut(s)	2430
BsrI	ACTGG	7 cut(s)	169, 1513, 1786, 2044, 2455, 2969, 3038
BssAI	RCCGGY	1 cut(s)	2293
BssECI	CCNNGG	8 cut(s)	120, 209, 1269, 2335, 2574, 2595, 2634, 3059
BssT1I	CCWWGG	4 cut(s)	1269, 2335, 2574, 3059
Bst2UI	CCWGG	2 cut(s)	121, 211
Bst6I	CTCTTC	3 cut(s)	1434, 1920, 2154
BstAPI	GCANNNNNTGC	1 cut(s)	1885
BstAUI	TGTACA	1 cut(s)	2430
BstBAI	YACGTR	1 cut(s)	239
BstBI	TTCGAA	1 cut(s)	660
BstC8I	GCNNGC	8 cut(s)	285, 453, 1247, 1330, 2873, 2947, 2983, 3020
BstDSI	CCRYGG	2 cut(s)	2595, 2634
BstENI	CCTNNNNNAGG	1 cut(s)	456
BstF5I	GGATG	9 cut(s)	715, 799, 835, 1006, 1535, 1847, 2172, 2761, 3053
BstFNI	CGCG	2 cut(s)	1877, 2190
BstH2I	RGCGCY	5 cut(s)	393, 721, 1463, 1790, 2300
BstHHI	GCGC	8 cut(s)	392, 720, 1026, 1462, 1789, 1879, 2299, 2485
BstMAI	GTCTC	4 cut(s)	18, 356, 1328, 2105
BstMWI	GCNNNNNNNGC	8 cut(s)	88, 104, 137, 387, 987, 1793, 1863, 1885
BstNI	CCWGG	2 cut(s)	121, 211
BstSCI	CCNGG	3 cut(s)	119, 209, 1978
BstSFI	CTRYAG	6 cut(s)	351, 1206, 2013, 2121, 2535, 2723
BstSLI	GKGCMC	1 cut(s)	210
BstUI	CGCG	2 cut(s)	1877, 2190
BstV1I	GCAGC	9 cut(s)	337, 600, 1192, 1220, 1723, 1999, 2467, 2491, 3008
BstV2I	GAAGAC	2 cut(s)	284, 1899
BstX2I	RGATCY	4 cut(s)	1966, 2201, 2331, 2738
BstXI	CCANNNNNNTGG	1 cut(s)	1225
BstYI	RGATCY	4 cut(s)	1966, 2201, 2331, 2738
BsuI	GTATCC	1 cut(s)	2079
BsuRI	GGCC	6 cut(s)	1245, 1589, 2253, 2594, 2693, 3010
BtgI	CCRYGG	2 cut(s)	2595, 2634
BtrI	CACGTC	2 cut(s)	205, 370
BtsCI	GGATG	9 cut(s)	715, 799, 835, 1006, 1535, 1847, 2172, 2761, 3053
BtsI	GCAGTG	1 cut(s)	2544
BtsIMutI	CAGTG	5 cut(s)	5, 1074, 2466, 2544, 2949
BveI	ACCTGC	2 cut(s)	31, 3043
Cac8I	GCNNGC	8 cut(s)	285, 453, 1247, 1330, 2873, 2947, 2983, 3020
CfoI	GCGC	8 cut(s)	392, 720, 1026, 1462, 1789, 1879, 2299, 2485
Cfr10I	RCCGGY	1 cut(s)	2293
Cfr13I	GGNCC	1 cut(s)	1587
CseI	GACGC	2 cut(s)	289, 2974
DraIII	CACNNNGTG	1 cut(s)	963
DrdI	GACNNNNNNGTC	1 cut(s)	720
DriI	GACNNNNNGTC	1 cut(s)	1982
DseDI	GACNNNNNNGTC	1 cut(s)	720
EaeI	YGGCCR	2 cut(s)	2592, 3008
Eam1104I	CTCTTC	3 cut(s)	1434, 1920, 2154
Eam1105I	GACNNNNNGTC	1 cut(s)	1982
EarI	CTCTTC	3 cut(s)	1434, 1920, 2154
Ecl136II	GAGCTC	3 cut(s)	981, 2361, 3071
Eco130I	CCWWGG	4 cut(s)	1269, 2335, 2574, 3059
Eco147I	AGGCCT	2 cut(s)	1245, 2693
Eco24I	GRGCYC	4 cut(s)	127, 983, 2363, 3073
Eco31I	GGTCTC	1 cut(s)	18
Eco32I	GATATC	1 cut(s)	2368
Eco53kI	GAGCTC	3 cut(s)	981, 2361, 3071
Eco57I	CTGAAG	2 cut(s)	2178, 2954
Eco72I	CACGTG	1 cut(s)	239
EcoICRI	GAGCTC	3 cut(s)	981, 2361, 3071
EcoNI	CCTNNNNNAGG	1 cut(s)	456
EcoRI	GAATTC	1 cut(s)	1145
EcoRII	CCWGG	2 cut(s)	119, 209
EcoRV	GATATC	1 cut(s)	2368
EcoT14I	CCWWGG	4 cut(s)	1269, 2335, 2574, 3059
EcoT22I	ATGCAT	1 cut(s)	411
EcoT38I	GRGCYC	4 cut(s)	127, 983, 2363, 3073
ErhI	CCWWGG	4 cut(s)	1269, 2335, 2574, 3059
FalI	AAGNNNNNCTT	4 cut(s)	871, 903, 1563, 1595
FaqI	GGGAC	2 cut(s)	2652, 2982
FauI	CCCGC	1 cut(s)	191
FbaI	TGATCA	3 cut(s)	271, 778, 856
FblI	GTMKAC	2 cut(s)	1975, 1998
FokI	GGATG	9 cut(s)	722, 806, 842, 1013, 1522, 1834, 2179, 2768, 3060
FriOI	GRGCYC	4 cut(s)	127, 983, 2363, 3073
FspBI	CTAG	2 cut(s)	536, 837
GlaI	GCGC	8 cut(s)	391, 719, 1025, 1461, 1788, 1878, 2298, 2484
GsuI	CTGGAG	1 cut(s)	2438
HaeII	RGCGCY	5 cut(s)	393, 721, 1463, 1790, 2300
HaeIII	GGCC	6 cut(s)	1245, 1589, 2253, 2594, 2693, 3010
HapII	CCGG	5 cut(s)	846, 900, 1861, 1979, 2294
HgaI	GACGC	2 cut(s)	289, 2974
HhaI	GCGC	8 cut(s)	392, 720, 1026, 1462, 1789, 1879, 2299, 2485
Hin6I	GCGC	8 cut(s)	390, 718, 1024, 1460, 1787, 1877, 2297, 2483
HinP1I	GCGC	8 cut(s)	390, 718, 1024, 1460, 1787, 1877, 2297, 2483
HincII	GTYRAC	1 cut(s)	2718
HindII	GTYRAC	1 cut(s)	2718
HindIII	AAGCTT	3 cut(s)	665, 1919, 2219
HinfI	GANTC	7 cut(s)	214, 833, 1234, 1983, 2557, 2664, 2991
HpaI	GTTAAC	1 cut(s)	2718
HpaII	CCGG	5 cut(s)	846, 900, 1861, 1979, 2294
HphI	GGTGA	9 cut(s)	109, 307, 764, 788, 922, 1441, 2145, 2199, 2566
Hpy166II	GTNNAC	7 cut(s)	1424, 1523, 1976, 1999, 2023, 2431, 2718
Hpy8I	GTNNAC	7 cut(s)	1424, 1523, 1976, 1999, 2023, 2431, 2718
Hpy99I	CGWCG	3 cut(s)	47, 793, 2990
HpyCH4IV	ACGT	4 cut(s)	204, 238, 369, 1401
HpyF10VI	GCNNNNNNNGC	8 cut(s)	88, 104, 137, 387, 987, 1793, 1863, 1885
HpySE526I	ACGT	4 cut(s)	204, 238, 369, 1401
HspAI	GCGC	8 cut(s)	390, 718, 1024, 1460, 1787, 1877, 2297, 2483
Ksp22I	TGATCA	3 cut(s)	271, 778, 856
KspAI	GTTAAC	1 cut(s)	2718
LmnI	GCTCC	3 cut(s)	185, 1733, 3076
Lsp1109I	GCAGC	9 cut(s)	337, 600, 1192, 1220, 1723, 1999, 2467, 2491, 3008
LweI	GCATC	5 cut(s)	396, 1856, 2278, 2402, 2535
MaeI	CTAG	2 cut(s)	536, 837
MaeII	ACGT	4 cut(s)	204, 238, 369, 1401
MbiI	CCGCTC	2 cut(s)	186, 993
MflI	RGATCY	4 cut(s)	1966, 2201, 2331, 2738
MhlI	GDGCHC	5 cut(s)	127, 210, 983, 2363, 3073
MlsI	TGGCCA	1 cut(s)	2594
MluI	ACGCGT	1 cut(s)	2188
MluNI	TGGCCA	1 cut(s)	2594
MlyI	GAGTC	2 cut(s)	842, 1992
MmeI	TCCRAC	5 cut(s)	660, 812, 1804, 2485, 2968
Mox20I	TGGCCA	1 cut(s)	2594
Mph1103I	ATGCAT	1 cut(s)	411
MroXI	GAANNNNTTC	3 cut(s)	57, 422, 2849
MscI	TGGCCA	1 cut(s)	2594
MseI	TTAA	7 cut(s)	549, 642, 2028, 2717, 2730, 3027, 3100
MslI	CAYNNNNRTG	6 cut(s)	203, 231, 692, 1223, 1451, 2030
Msp20I	TGGCCA	1 cut(s)	2594
MspI	CCGG	5 cut(s)	846, 900, 1861, 1979, 2294
MspR9I	CCNGG	3 cut(s)	121, 211, 1980
Mva1269I	GAATGC	4 cut(s)	814, 1890, 3033, 3077
MvaI	CCWGG	2 cut(s)	121, 211
MvnI	CGCG	2 cut(s)	1877, 2190
MwoI	GCNNNNNNNGC	8 cut(s)	88, 104, 137, 387, 987, 1793, 1863, 1885
NciI	CCSGG	1 cut(s)	1980
NlaIV	GGNNCC	8 cut(s)	430, 786, 1911, 1968, 2333, 2341, 2649, 3057
NmuCI	GTSAC	6 cut(s)	3, 239, 910, 937, 2266, 2401
NsiI	ATGCAT	1 cut(s)	411
NspV	TTCGAA	1 cut(s)	660
PceI	AGGCCT	2 cut(s)	1245, 2693
PcsI	WCGNNNNNNNCGW	1 cut(s)	2603
PctI	GAATGC	4 cut(s)	814, 1890, 3033, 3077
PdmI	GAANNNNTTC	3 cut(s)	57, 422, 2849
PfeI	GAWTC	5 cut(s)	214, 1234, 2557, 2664, 2991
PleI	GAGTC	2 cut(s)	841, 1991
PmaCI	CACGTG	1 cut(s)	239
PmlI	CACGTG	1 cut(s)	239
PpsI	GAGTC	2 cut(s)	841, 1991
Ppu21I	YACGTR	1 cut(s)	239
PsiI	TTATAA	1 cut(s)	506
Psp124BI	GAGCTC	3 cut(s)	983, 2363, 3073
Psp1406I	AACGTT	1 cut(s)	1401
Psp6I	CCWGG	2 cut(s)	119, 209
PspCI	CACGTG	1 cut(s)	239
PspGI	CCWGG	2 cut(s)	119, 209
PspN4I	GGNNCC	8 cut(s)	430, 786, 1911, 1968, 2333, 2341, 2649, 3057
PspPI	GGNCC	1 cut(s)	1587
PstI	CTGCAG	4 cut(s)	355, 1210, 2017, 2539
PsuI	RGATCY	4 cut(s)	1966, 2201, 2331, 2738
RseI	CAYNNNNRTG	6 cut(s)	203, 231, 692, 1223, 1451, 2030
SacI	GAGCTC	3 cut(s)	983, 2363, 3073
SaqAI	TTAA	7 cut(s)	549, 642, 2028, 2717, 2730, 3027, 3100
Sau96I	GGNCC	1 cut(s)	1587
ScaI	AGTACT	1 cut(s)	1714
SchI	GAGTC	2 cut(s)	842, 1992
ScrFI	CCNGG	3 cut(s)	121, 211, 1980
SduI	GDGCHC	5 cut(s)	127, 210, 983, 2363, 3073
SfaNI	GCATC	5 cut(s)	396, 1856, 2278, 2402, 2535
SfcI	CTRYAG	6 cut(s)	351, 1206, 2013, 2121, 2535, 2723
SfuI	TTCGAA	1 cut(s)	660
SgrAI	CRCCGGYG	1 cut(s)	2293
SmiMI	CAYNNNNRTG	6 cut(s)	203, 231, 692, 1223, 1451, 2030
SmlI	CTYRAG	3 cut(s)	311, 359, 2864
SmoI	CTYRAG	3 cut(s)	311, 359, 2864
SseBI	AGGCCT	2 cut(s)	1245, 2693
SsiI	CCGC	8 cut(s)	82, 91, 184, 378, 449, 991, 1680, 2072
SspI	AATATT	2 cut(s)	2440, 3098
SspMI	CTAG	2 cut(s)	536, 837
SstI	GAGCTC	3 cut(s)	983, 2363, 3073
StuI	AGGCCT	2 cut(s)	1245, 2693
StyD4I	CCNGG	3 cut(s)	119, 209, 1978
StyI	CCWWGG	4 cut(s)	1269, 2335, 2574, 3059
TaiI	ACGT	4 cut(s)	207, 241, 372, 1404
TaqI	TCGA	7 cut(s)	16, 45, 340, 660, 983, 2127, 2178
TatI	WGTACW	2 cut(s)	1712, 2430
TauI	GCSGC	2 cut(s)	381, 993
TfiI	GAWTC	5 cut(s)	214, 1234, 2557, 2664, 2991
Tru1I	TTAA	7 cut(s)	549, 642, 2028, 2717, 2730, 3027, 3100
Tru9I	TTAA	7 cut(s)	549, 642, 2028, 2717, 2730, 3027, 3100
TscAI	CASTG	5 cut(s)	12, 1074, 2473, 2544, 2956
TseFI	GTSAC	6 cut(s)	3, 239, 910, 937, 2266, 2401
TseI	GCWGC	9 cut(s)	350, 588, 1205, 1208, 1736, 2012, 2480, 2504, 2996
Tsp45I	GTSAC	6 cut(s)	3, 239, 910, 937, 2266, 2401
TspGWI	ACGGA	5 cut(s)	806, 1330, 1959, 2612, 2660
TspRI	CASTG	5 cut(s)	12, 1074, 2473, 2544, 2956
XagI	CCTNNNNNAGG	1 cut(s)	456
XapI	RAATTY	3 cut(s)	1145, 1528, 2927
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1225, 1840, 2749, 3045
XmiI	GTMKAC	2 cut(s)	1975, 1998
XmnI	GAANNNNTTC	3 cut(s)	57, 422, 2849
XspI	CTAG	2 cut(s)	536, 837
ZrmI	AGTACT	1 cut(s)	1714
Zsp2I	ATGCAT	1 cut(s)	411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.