Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000174

Physical Location & Seq

Genomic Coordinates Reverse (-)

53016145 .. 53019042

Gene Structure 2898 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1429900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGAACTTTGTCACCTCGACAATGGCTTCAAAATTGATAACAAATGCCTCACTCAACTCATGAAGGAGAAAAACCTACACCTTCTTCAAGAGCTCGAAGGCATTGATGGCGTTGTATCGGCTCTCCAAACCGACACTGAGCGTGGCATCGACGATGATGGTGACAATATTGCTCGCCGGCTTGAAGCATTCGGTTCAAACACGTACAAGAAGCCGCCTTCAAAAAGCCTCTTCCATTTTATGTGGCAAGCTTCTAAGGATCTTACCATTGTCATCCTCTTAGGCTGCGCCGCGCTCTCACTTGGATTCGGCATAAAGCAGCATGGACTAAAAGAAGGATGGTATGACGGAGGAAGCATCTTTGTCGCGGTTTTTCTTATCGTGGTTGTATCCGCCCTTAGTGACTACAGGCAGAGCAAGCAGTTCCGGAAGTTGTCCAAAGCAAGTGAGGACATTCCAATTGATGTTGTGAGAGGAGGTAGGCGGCGACAGATTTCGAACCTTGGTATTGTTGTTGGAGATGTTGTGTTCTTGAAAATTGGAGATCAAGTTCCTGCTGATGGATTGTTCTTGCGTGGACATTCGTTGAGCGTGGACGAATCAAGCATGACGGGGGAAAGTGAGCATGTGGAAGTTAATGGCGGCCTGAATCCGTTCTTGTTTTCAGGAACCAAGGTGGTCGATGGATATGGTGAATTGCTTGTGGCTTCAGTTGGCATGAACTCGACGTGGGGCGAAATGATGAGCTTGATTAGCCGTGACACCAGTGATCAGCAGACACCATTACAAGCGCGGCTAAGTAAACTAACCCCGCTGATGGCGAAGATTGGCTTGGGTGTTGCATTCCTAGTTTTCGTGGTCTTGGTAGCGCGTTACTTCACAGGCAACACGAAGAACAACAGTGAATTTGGTAAGTTTGAGGGCAACAAGACAAAGATTGATGATATGGTGAATGCCGCGGTGGACATGGTAGCAGTTGCAGTTATTATTGTCGTGATTGCAATTCCTGAAGGCTTCCCGTTGGCTGTCACTCTGACTCTCGCTTATTCGATGAAGAAGATGATGGCGGACCAAGCATTGGTAAGGAGGCTCTCTGCTTGCGAAACTATGGGATGTGCCACCGTAATTTGTACAGACAAGACGGGCACACTGACGCTCAACGAGATGAAGGTTACAAAGTTTTGGCTTGGACAGGAATCTGTGGAAGATGATGCTGGTTATTCATCAATTTCTGATTGCCTTCTCGATTTAATCCGAGAAGGAGTAACGTTTAACACAACTGGTAGTGTTTATAGGCCTAGTTCAGGATCGGGATTTGAGTTTTCGGGCAGTCCTACTGAAAAAGCCATTCTTTCATGGGCTACTCTAGAGCTGCACATGGACATGGAGAAATTGAAGAAGCAATGTGTCATTCAACATGTTGAAGTCTTCAATTCGCACAAGAAACGAAGTGGGGTTTTGATGAGAAGGAAGGCCGACAATACTACCCATGTACATTGGAAAGGAGCTGCAGAAATGATACTAGAAATATGCTCGAGTTACTACGATGCGTCTGGTGTCACGAAAGATCTGAATGATGATGAAAGGCTGAAGTTTGAGAAGATTATTCAAGGCATGGCAGCAAGCAGTCTCCGATGCATTGCTTTTGCACACAAACAAGTTCAGAATAAGCACATTAATGACGAAAAGTACGAAAAGCTCGAAGAAAGCAACTTGATCCTCGTAGGATTGGTAGGTCTAAAGGATCCATGTAGGCCAGGAGTGAGGGAAGCAGTAGAAGCTTGCCAGTTAGCTGGGGTGCAGGTGAAAATGATCACCGGTGACAATGTTTTCACCGCTAAAGCAATAGCTAGCGAGTGCGGGATACTCCGGCCTGACCAGGAAGCCAAGGAAGCAGTGGTAGAAGGTATAGAATTTCGCAACTTCACGCCGGAGCAAAGAATGCAGAAAGTTGATGGAATTCACGTGATGGCGAGGTCTTCGCCATTTGATAAGCTTCTTATGGTGCAATGCTTGAAACAGAAAGGCAATGTAGTAGCTGTGACAGGTGATGGCACGAATGATGCACCCGCACTCAAAGAAGCGGACATAGGACTCTCGATGGGGATTCAGGGAACGGAAGTGGCCAAGGAGAGCTCAGACATTGTCATTTTAGATGACAATTTTGCAACTTTGGTGACTGTTTTAAAGTGGGGAAGAGGAGTTTATGCCAACATCCAAAAGTTTGTTCAATTCCAGCTCACAATAAATGTTGCAACTCTTGTGGTCAACTTTGTGGCAGCTGCCTCGGCTGGCGAAGTACCTTTAACAGCGGTCCAGCTACTGTGGGTGAATCTGATTATGGACACAATGGCGGCTCTGGCTCTCGCCACCGATAAGCCCACCAAAGAGCTCATGGAAAAGCCACCCGTGGGTCAGACGGATCCCGTGATCACCAACATCATGTGGAGGAACCTTTTACCCCAAGCTTTGTTCCAGATAGTTGTGCTCTTGGTCTTGCAGTTCAAAGGCAGATCAATATTTGGTGTTAGTGAGACGGTAAACAACACATTGATCTTCAATGTATTTGTGTTTTGCCAAGTGTTTAATGAGCTTAATTCAAGGGATATGGAGAGGATTAATATCTTCAAAGGGAGTCAGGGGAACAGGCTGTTTTGGGGCATCCTCGTTGTAACAATTGCAGTTCAGGTGGTGATGGTGGAACTTCTGAACAAGTTTGCAAACACAGAGCGGTTAAGTTGGGGACAATGGGGTGCATGCATTGGGATTGCAGCCATATCTTGGCCAATTAGTTGGGTTTTCAAGTTCATACCAGTACCTGCTAAGCCAATATTCAGCTATCTAAAGAGCGATAGCAAACGTGGGTTGGAGCAGTTGGCCGAGGCAAAGTCCCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

966

Amino Acids

106.07

Weight (kDa)

6.07

Isoelectric Point (pI)

29.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	33 - 100	1.8e-13	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	155 - 251	1.3e-19	P-type ATPase actuator domain
Hydrolase	PF00702	375 - 696	1.4e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	416 - 586	2.8e-31	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	767 - 937	3e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22910
fragaria_vesca	FvH4_4g01640 FvH4_4g01670 FvH4_4g01690 FvH4_4g01690 FvH4_4g01692 FvH4_4g06940
malus_domestica	MD04G1223800.v1.1 MD12G1240600.v1.1 MD13G1201600.v1.1 MD15G1425600.v1.1 MD15G1425900.v1.1 MD15G1426000.v1.1 MD15G1426200.v1.1 MD15G1429900.v1.1 MD16G1186200.v1.1 MD16G1201500.v1.1 MD16G1201800.v1.1 MD16G1245900.v1.1
prunus_persica	Prupe.1G023100_v2.0.a1 Prupe.1G023200_v2.0.a1 Prupe.1G023300_v2.0.a1 Prupe.1G023400_v2.0.a1 Prupe.2G086400_v2.0.a1
pyrus_communis	pycom03g20400 pycom04g03220 pycom04g03230 pycom04g19720 pycom13g17500 pycom14g13160 pycom15g37720
rosa_chinensis	RchiOBHm_Chr4g0385871 RchiOBHm_Chr4g0388251 RchiOBHm_Chr4g0388261 RchiOBHm_Chr4g0388291 RchiOBHm_Chr4g0388301 RchiOBHm_Chr4g0388321 RchiOBHm_Chr4g0388331 RchiOBHm_Chr4g0388341 RchiOBHm_Chr4g0388491 RchiOBHm_Chr4g0388501 RchiOBHm_Chr4g0388521 RchiOBHm_Chr4g0388541 RchiOBHm_Chr4g0388581 RchiOBHm_Chr4g0388591 RchiOBHm_Chr5g0038901 RchiOBHm_Chr5g0039001 RchiOBHm_Chr6g0264011
rosa_laevigata	RLG00000003378 RLG00000003379 RLG00000010036 RLG00000010037 RLG00000010039 RLG00000010041 RLG00000010042 RLG00000010043 RLG00000010049 RLG00000010050 RLG00000010051 RLG00000010052 RLG00000010054 RLG00000010055 RLG00000010062 RLG00000010064 RLG00000010065 RLG00000010066 RLG00000010067 RLG00000010069 RLG00000010070 RLG00000010071 RLG00000014295 RLG00000033880
rosa_multiflora	Rmu_co8110336.1_g000001 Rmu_sc0000533.1_g000070 Rmu_sc0000533.1_g000082 Rmu_sc0000753.1_g000013 Rmu_sc0000753.1_g000024 Rmu_sc0000753.1_g000027 Rmu_sc0000753.1_g000029 Rmu_sc0000753.1_g000030 Rmu_sc0000753.1_g000037 Rmu_sc0000753.1_g000048 Rmu_sc0001241.1_g000003 Rmu_sc0001241.1_g000020 Rmu_sc0001241.1_g000034 Rmu_sc0001241.1_g000045 Rmu_sc0001949.1_g000015 Rmu_sc0001949.1_g000020 Rmu_sc0001949.1_g000023 Rmu_sc0001949.1_g000028 Rmu_sc0002357.1_g000005 Rmu_sc0002357.1_g000017 Rmu_sc0002357.1_g000032 Rmu_sc0002357.1_g000035 Rmu_sc0002357.1_g000043 Rmu_sc0002694.1_g000002 Rmu_sc0004459.1_g000018 Rmu_sc0004773.1_g000011 Rmu_sc0006339.1_g000009 Rmu_sc0008728.1_g000008 Rmu_sc0012275.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042120 Rroxscaffold_2G00113240 Rroxscaffold_2G00131810 Rroxscaffold_4G00314870 Rroxscaffold_5G00334730 Rroxscaffold_5G00334750 Rroxscaffold_5G00334770 Rroxscaffold_5G00334790 Rroxscaffold_5G00334850 Rroxscaffold_5G00334970 Rroxscaffold_5G00335010 Rroxscaffold_5G00335030 Rroxscaffold_5G00335040
rosa_rugosa	Rorug03G0301800 Rorug03G0316600 Rorug03G0316600 Rorug03G0316700 Rorug03G0316700 Rorug03G0316800.1 Rorug03G0317300 Rorug03G0317400 Rorug03G0318000 Rorug03G0318500 Rorug03G0318600 Rorug03G0318700 Rorug03G0319000 Rorug03G0319000 Rorug03G0319100 Rorug05G0175000 Rorug05G0175500
rosa_samantha	Rh4CG007500 Rh4CG022300 Rh4CG022400 Rh4CG022600 Rh4CG022700 Rh4CG023500 Rh4CG023700 Rh4CG023900 Rh4CG024000 Rh4CG024600 Rh4CG025200 Rh4CG025300 Rh4CG025700 Rh4CG025800 Rh4CG025900 Rh4CG026000 Rh4CG026100 Rh4CG026200 Rh5BG266500 Rh6AG128500 Rh6DG110800
rosa_wichuraiana	Rw0G009370 Rw4G000360 Rw4G001430 Rw4G001440 Rw4G001460 Rw4G001470 Rw4G001490 Rw4G001500 Rw4G001520 Rw4G001620 Rw4G001630 Rw4G001650 Rw4G001670 Rw5G024450 Rw5G024490 Rw6G011090 Rw6G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1792
Acc36I	ACCTGC	2 cut(s)	1792, 2827
AccB7I	CCANNNNNTGG	2 cut(s)	1078, 2781
AccBSI	CCGCTC	1 cut(s)	2731
AccII	CGCG	5 cut(s)	293, 368, 793, 871, 959
AccIII	TCCGGA	1 cut(s)	426
AclI	AACGTT	1 cut(s)	1268
AclWI	GGATC	7 cut(s)	267, 1315, 1711, 1739, 1752, 2417, 2430
AcoI	YGGCCR	3 cut(s)	2124, 2783, 2877
AcsI	RAATTY	3 cut(s)	905, 1913, 1959
AcuI	CTGAAG	3 cut(s)	693, 1029, 1610
AcvI	CACGTG	1 cut(s)	1966
AfaI	GTAC	6 cut(s)	206, 1132, 1494, 1691, 2301, 2817
AfiI	CCNNNNNNNGG	6 cut(s)	560, 817, 1078, 1304, 2411, 2781
AflIII	ACRYGT	2 cut(s)	201, 1417
AgeI	ACCGGT	1 cut(s)	1817
AjiI	CACGTC	1 cut(s)	729
AjnI	CCWGG	2 cut(s)	1756, 1878
AjuI	GAANNNNNNNTTGG	4 cut(s)	815, 847, 2211, 2243
AloI	GAACNNNNNNTCC	2 cut(s)	534, 566
Alw21I	GWGCWC	4 cut(s)	96, 2138, 2394, 2490
Alw26I	GTCTC	2 cut(s)	1634, 2528
AlwI	GGATC	7 cut(s)	267, 1315, 1711, 1739, 1752, 2417, 2430
AlwNI	CAGNNNCTG	2 cut(s)	2323, 2819
Ama87I	CYCGRG	1 cut(s)	1534
Aor13HI	TCCGGA	1 cut(s)	426
AoxI	GGCC	8 cut(s)	643, 1295, 1473, 1754, 1871, 2124, 2783, 2877
ApeKI	GCWGC	8 cut(s)	285, 319, 1372, 1508, 1619, 2279, 2282, 2771
ApoI	RAATTY	3 cut(s)	905, 1913, 1959
AseI	ATTAAT	2 cut(s)	1677, 2619
AsiGI	ACCGGT	1 cut(s)	1817
Asp700I	GAANNNNTTC	1 cut(s)	653
AspLEI	GCGC	4 cut(s)	290, 295, 793, 871
AspS9I	GGNCC	2 cut(s)	1069, 2314
AsuII	TTCGAA	1 cut(s)	497
AsuNHI	GCTAGC	1 cut(s)	1850
AvaI	CYCGRG	1 cut(s)	1534
AvaII	GGWCC	2 cut(s)	1069, 2314
BaeGI	GKGCMC	1 cut(s)	1148
BalI	TGGCCA	2 cut(s)	2126, 2785
BamHI	GGATCC	2 cut(s)	1744, 2422
BanII	GRGCYC	3 cut(s)	96, 2138, 2394
BbrPI	CACGTG	1 cut(s)	1966
BbsI	GAAGAC	2 cut(s)	1420, 1971
Bbv12I	GWGCWC	4 cut(s)	96, 2138, 2394, 2490
BbvI	GCAGC	8 cut(s)	272, 331, 1359, 1495, 1631, 2269, 2291, 2783
BceAI	ACGGC	1 cut(s)	741
BcgI	CGANNNNNNTGC	4 cut(s)	346, 380, 2268, 2302
BciT130I	CCWGG	2 cut(s)	1758, 1880
BciVI	GTATCC	2 cut(s)	400, 1857
BclI	TGATCA	3 cut(s)	769, 1812, 2430
BcoDI	GTCTC	2 cut(s)	1634, 2528
BfaI	CTAG	5 cut(s)	848, 1299, 1367, 1523, 1851
BfmI	CTRYAG	2 cut(s)	406, 1509
BfuAI	ACCTGC	2 cut(s)	1792, 2827
BfuI	GTATCC	2 cut(s)	400, 1857
BglII	AGATCT	1 cut(s)	1567
BlpI	GCTNAGC	1 cut(s)	2823
Bme1390I	CCNGG	2 cut(s)	1758, 1880
Bme18I	GGWCC	2 cut(s)	1069, 2314
BmeT110I	CYCGRG	1 cut(s)	1534
BmgBI	CACGTC	1 cut(s)	729
BmgT120I	GGNCC	2 cut(s)	1069, 2314
BmiI	GGNNCC	4 cut(s)	670, 1746, 2424, 2453
BmrFI	CCNGG	2 cut(s)	1758, 1880
BmsI	GCATC	7 cut(s)	156, 366, 1201, 1537, 1625, 2053, 2670
BmtI	GCTAGC	1 cut(s)	1854
BpiI	GAAGAC	2 cut(s)	1420, 1971
Bpu1102I	GCTNAGC	1 cut(s)	2823
Bpu14I	TTCGAA	1 cut(s)	497
BsaAI	YACGTR	2 cut(s)	204, 1966
BsaBI	GATNNNNATC	1 cut(s)	2621
BsaJI	CCNNGG	8 cut(s)	502, 672, 957, 1887, 2127, 2286, 2409, 2880
BsaWI	WCCGGW	2 cut(s)	426, 1817
BsaXI	ACNNNNNCTCC	4 cut(s)	510, 534, 540, 564
Bsc4I	CCNNNNNNNGG	6 cut(s)	560, 817, 1078, 1304, 2411, 2781
Bse118I	RCCGGY	2 cut(s)	177, 1817
Bse1I	ACTGG	4 cut(s)	765, 1285, 1786, 2813
Bse3DI	GCAATG	4 cut(s)	1409, 1638, 2015, 2035
Bse8I	GATNNNNATC	1 cut(s)	2621
BseAI	TCCGGA	1 cut(s)	426
BseBI	CCWGG	2 cut(s)	1758, 1880
BseDI	CCNNGG	8 cut(s)	502, 672, 957, 1887, 2127, 2286, 2409, 2880
BseGI	GGATG	5 cut(s)	273, 344, 1118, 2214, 2661
BseJI	GATNNNNATC	1 cut(s)	2621
BseLI	CCNNNNNNNGG	6 cut(s)	560, 817, 1078, 1304, 2411, 2781
BseMI	GCAATG	4 cut(s)	1409, 1638, 2015, 2035
BseMII	CTCAG	2 cut(s)	129, 2151
BseNI	ACTGG	4 cut(s)	765, 1285, 1786, 2813
BseRI	GAGGAG	2 cut(s)	489, 2214
BseSI	GKGCMC	1 cut(s)	1148
BseXI	GCAGC	8 cut(s)	272, 331, 1359, 1495, 1631, 2269, 2291, 2783
BseYI	CCCAGC	1 cut(s)	1793
BsgI	GTGCAG	2 cut(s)	1358, 1820
Bsh1236I	CGCG	5 cut(s)	293, 368, 793, 871, 959
BshFI	GGCC	8 cut(s)	645, 1297, 1475, 1756, 1873, 2126, 2785, 2879
BshTI	ACCGGT	1 cut(s)	1817
BsiHKAI	GWGCWC	4 cut(s)	96, 2138, 2394, 2490
BsiHKCI	CYCGRG	1 cut(s)	1534
BsiSI	CCGG	5 cut(s)	178, 427, 1818, 1870, 1931
BslFI	GGGAC	2 cut(s)	2757, 2875
BslI	CCNNNNNNNGG	6 cut(s)	560, 817, 1078, 1304, 2411, 2781
BsmAI	GTCTC	2 cut(s)	1634, 2528
BsmBI	CGTCTC	1 cut(s)	2528
BsmFI	GGGAC	2 cut(s)	2757, 2875
BsmI	GAATGC	4 cut(s)	188, 842, 958, 1947
BsnI	GGCC	8 cut(s)	645, 1297, 1475, 1756, 1873, 2126, 2785, 2879
BsoBI	CYCGRG	1 cut(s)	1534
Bsp119I	TTCGAA	1 cut(s)	497
Bsp1286I	GDGCHC	5 cut(s)	96, 1148, 2138, 2394, 2490
Bsp13I	TCCGGA	1 cut(s)	426
Bsp1407I	TGTACA	2 cut(s)	1130, 1492
Bsp1720I	GCTNAGC	1 cut(s)	2823
BspANI	GGCC	8 cut(s)	645, 1297, 1475, 1756, 1873, 2126, 2785, 2879
BspCNI	CTCAG	2 cut(s)	130, 2150
BspEI	TCCGGA	1 cut(s)	426
BspFNI	CGCG	5 cut(s)	293, 368, 793, 871, 959
BspHI	TCATGA	1 cut(s)	60
BspLI	GGNNCC	4 cut(s)	670, 1746, 2424, 2453
BspMAI	CTGCAG	1 cut(s)	1513
BspMI	ACCTGC	2 cut(s)	1792, 2827
BspOI	GCTAGC	1 cut(s)	1854
BspPI	GGATC	7 cut(s)	267, 1315, 1711, 1739, 1752, 2417, 2430
BspT104I	TTCGAA	1 cut(s)	497
BsrBI	CCGCTC	1 cut(s)	2731
BsrDI	GCAATG	4 cut(s)	1409, 1638, 2015, 2035
BsrFI	RCCGGY	2 cut(s)	177, 1817
BsrGI	TGTACA	2 cut(s)	1130, 1492
BsrI	ACTGG	4 cut(s)	765, 1285, 1786, 2813
BssAI	RCCGGY	2 cut(s)	177, 1817
BssECI	CCNNGG	8 cut(s)	502, 672, 957, 1887, 2127, 2286, 2409, 2880
BssT1I	CCWWGG	4 cut(s)	502, 672, 1887, 2127
Bst2UI	CCWGG	2 cut(s)	1758, 1880
Bst4CI	ACNGT	5 cut(s)	902, 1123, 2182, 2325, 2539
Bst6I	CTCTTC	2 cut(s)	236, 2191
BstAPI	GCANNNNNTGC	1 cut(s)	1942
BstAUI	TGTACA	2 cut(s)	1130, 1492
BstBAI	YACGTR	2 cut(s)	204, 1966
BstBI	TTCGAA	1 cut(s)	497
BstDEI	CTNAG	7 cut(s)	138, 255, 280, 398, 797, 2137, 2823
BstDSI	CCRYGG	2 cut(s)	957, 2409
BstENI	CCTNNNNNAGG	1 cut(s)	1302
BstF5I	GGATG	5 cut(s)	273, 344, 1118, 2214, 2661
BstFNI	CGCG	5 cut(s)	293, 368, 793, 871, 959
BstHHI	GCGC	4 cut(s)	290, 295, 793, 871
BstMAI	GTCTC	2 cut(s)	1634, 2528
BstMWI	GCNNNNNNNGC	9 cut(s)	108, 418, 753, 1073, 1778, 1892, 1942, 2288, 2360
BstNI	CCWGG	2 cut(s)	1758, 1880
BstNSI	RCATGY	3 cut(s)	629, 1421, 2760
BstSCI	CCNGG	2 cut(s)	1756, 1878
BstSFI	CTRYAG	2 cut(s)	406, 1509
BstSLI	GKGCMC	1 cut(s)	1148
BstUI	CGCG	5 cut(s)	293, 368, 793, 871, 959
BstV1I	GCAGC	8 cut(s)	272, 331, 1359, 1495, 1631, 2269, 2291, 2783
BstV2I	GAAGAC	2 cut(s)	1420, 1971
BstX2I	RGATCY	4 cut(s)	259, 1567, 1744, 2422
BstXI	CCANNNNNNTGG	2 cut(s)	1793, 2793
BstYI	RGATCY	4 cut(s)	259, 1567, 1744, 2422
BsuI	GTATCC	2 cut(s)	400, 1857
BsuRI	GGCC	8 cut(s)	645, 1297, 1475, 1756, 1873, 2126, 2785, 2879
BtgI	CCRYGG	2 cut(s)	957, 2409
BtrI	CACGTC	1 cut(s)	729
BtsCI	GGATG	5 cut(s)	273, 344, 1118, 2214, 2661
BtsI	GCAGTG	1 cut(s)	1902
BtsIMutI	CAGTG	5 cut(s)	135, 772, 907, 1148, 1902
BveI	ACCTGC	2 cut(s)	1792, 2827
CaiI	CAGNNNCTG	2 cut(s)	2323, 2819
CciI	TCATGA	1 cut(s)	60
CfoI	GCGC	4 cut(s)	290, 295, 793, 871
Cfr10I	RCCGGY	2 cut(s)	177, 1817
Cfr13I	GGNCC	2 cut(s)	1069, 2314
Cfr42I	CCGCGG	1 cut(s)	960
CseI	GACGC	2 cut(s)	1162, 1539
Csp6I	GTAC	6 cut(s)	205, 1131, 1493, 1690, 2300, 2816
CspAI	ACCGGT	1 cut(s)	1817
CspCI	CAANNNNNGTGG	2 cut(s)	2244, 2279
CviQI	GTAC	6 cut(s)	205, 1131, 1493, 1690, 2300, 2816
DdeI	CTNAG	7 cut(s)	138, 255, 280, 398, 797, 2137, 2823
DraI	TTTAAA	1 cut(s)	2187
EaeI	YGGCCR	3 cut(s)	2124, 2783, 2877
Eam1104I	CTCTTC	2 cut(s)	236, 2191
EarI	CTCTTC	2 cut(s)	236, 2191
EciI	GGCGGA	2 cut(s)	382, 1082
Ecl136II	GAGCTC	3 cut(s)	94, 2136, 2392
Eco130I	CCWWGG	4 cut(s)	502, 672, 1887, 2127
Eco147I	AGGCCT	1 cut(s)	1297
Eco24I	GRGCYC	3 cut(s)	96, 2138, 2394
Eco47I	GGWCC	2 cut(s)	1069, 2314
Eco53kI	GAGCTC	3 cut(s)	94, 2136, 2392
Eco57I	CTGAAG	3 cut(s)	693, 1029, 1610
Eco72I	CACGTG	1 cut(s)	1966
Eco88I	CYCGRG	1 cut(s)	1534
EcoICRI	GAGCTC	3 cut(s)	94, 2136, 2392
EcoNI	CCTNNNNNAGG	1 cut(s)	1302
EcoRI	GAATTC	1 cut(s)	1959
EcoRII	CCWGG	2 cut(s)	1756, 1878
EcoT14I	CCWWGG	4 cut(s)	502, 672, 1887, 2127
EcoT22I	ATGCAT	2 cut(s)	1640, 2762
EcoT38I	GRGCYC	3 cut(s)	96, 2138, 2394
ErhI	CCWWGG	4 cut(s)	502, 672, 1887, 2127
Esp3I	CGTCTC	1 cut(s)	2528
FalI	AAGNNNNNCTT	4 cut(s)	815, 847, 1335, 1367
FaqI	GGGAC	2 cut(s)	2757, 2875
FauI	CCCGC	3 cut(s)	819, 1853, 2077
FbaI	TGATCA	3 cut(s)	769, 1812, 2430
FokI	GGATG	5 cut(s)	260, 351, 1125, 2201, 2648
FriOI	GRGCYC	3 cut(s)	96, 2138, 2394
FspBI	CTAG	5 cut(s)	848, 1299, 1367, 1523, 1851
GlaI	GCGC	4 cut(s)	289, 294, 792, 870
GsaI	CCCAGC	1 cut(s)	1797
HaeIII	GGCC	8 cut(s)	645, 1297, 1475, 1756, 1873, 2126, 2785, 2879
HapII	CCGG	5 cut(s)	178, 427, 1818, 1870, 1931
HgaI	GACGC	2 cut(s)	1162, 1539
HhaI	GCGC	4 cut(s)	290, 295, 793, 871
Hin6I	GCGC	4 cut(s)	288, 293, 791, 869
HinP1I	GCGC	4 cut(s)	288, 293, 791, 869
HincII	GTYRAC	1 cut(s)	2269
HindII	GTYRAC	1 cut(s)	2269
HindIII	AAGCTT	4 cut(s)	249, 1779, 1994, 2466
HinfI	GANTC	9 cut(s)	306, 599, 649, 1036, 1196, 2094, 2107, 2332, 2635
HpaII	CCGG	5 cut(s)	178, 427, 1818, 1870, 1931
Hpy166II	GTNNAC	6 cut(s)	578, 595, 803, 964, 2269, 2542
Hpy8I	GTNNAC	6 cut(s)	578, 595, 803, 964, 2269, 2542
Hpy99I	CGWCG	2 cut(s)	155, 730
HpyCH4III	ACNGT	5 cut(s)	902, 1123, 2182, 2325, 2539
HpyCH4IV	ACGT	5 cut(s)	203, 728, 1268, 1965, 2860
HpyF10VI	GCNNNNNNNGC	9 cut(s)	108, 418, 753, 1073, 1778, 1892, 1942, 2288, 2360
HpyF3I	CTNAG	7 cut(s)	138, 255, 280, 398, 797, 2137, 2823
HpySE526I	ACGT	5 cut(s)	203, 728, 1268, 1965, 2860
HspAI	GCGC	4 cut(s)	288, 293, 791, 869
Kpn2I	TCCGGA	1 cut(s)	426
KroI	GCCGGC	1 cut(s)	177
KroNI	GCCGGC	1 cut(s)	179
Ksp22I	TGATCA	3 cut(s)	769, 1812, 2430
KspI	CCGCGG	1 cut(s)	960
LmnI	GCTCC	3 cut(s)	1505, 1933, 2869
Lsp1109I	GCAGC	8 cut(s)	272, 331, 1359, 1495, 1631, 2269, 2291, 2783
LweI	GCATC	7 cut(s)	156, 366, 1201, 1537, 1625, 2053, 2670
MaeI	CTAG	5 cut(s)	848, 1299, 1367, 1523, 1851
MaeII	ACGT	5 cut(s)	203, 728, 1268, 1965, 2860
MbiI	CCGCTC	1 cut(s)	2731
MfeI	CAATTG	2 cut(s)	459, 2676
MflI	RGATCY	4 cut(s)	259, 1567, 1744, 2422
MhlI	GDGCHC	5 cut(s)	96, 1148, 2138, 2394, 2490
MlsI	TGGCCA	2 cut(s)	2126, 2785
MluNI	TGGCCA	2 cut(s)	2126, 2785
MlyI	GAGTC	3 cut(s)	1030, 2088, 2644
MmeI	TCCRAC	2 cut(s)	496, 2847
Mox20I	TGGCCA	2 cut(s)	2126, 2785
Mph1103I	ATGCAT	2 cut(s)	1640, 2762
MroI	TCCGGA	1 cut(s)	426
MroNI	GCCGGC	1 cut(s)	177
MroXI	GAANNNNTTC	1 cut(s)	653
MscI	TGGCCA	2 cut(s)	2126, 2785
MslI	CAYNNNNRTG	2 cut(s)	1382, 1677
Msp20I	TGGCCA	2 cut(s)	2126, 2785
MspA1I	CMGCKG	4 cut(s)	814, 959, 2282, 2312
MspI	CCGG	5 cut(s)	178, 427, 1818, 1870, 1931
MspR9I	CCNGG	2 cut(s)	1758, 1880
MteI	GCGCNGCGC	1 cut(s)	291
MunI	CAATTG	2 cut(s)	459, 2676
Mva1269I	GAATGC	4 cut(s)	188, 842, 958, 1947
MvaI	CCWGG	2 cut(s)	1758, 1880
MvnI	CGCG	5 cut(s)	293, 368, 793, 871, 959
MwoI	GCNNNNNNNGC	9 cut(s)	108, 418, 753, 1073, 1778, 1892, 1942, 2288, 2360
NaeI	GCCGGC	1 cut(s)	179
NgoMIV	GCCGGC	1 cut(s)	177
NheI	GCTAGC	1 cut(s)	1850
NlaIV	GGNNCC	4 cut(s)	670, 1746, 2424, 2453
NmeAIII	GCCGAG	1 cut(s)	2267
NmuCI	GTSAC	9 cut(s)	11, 161, 401, 758, 1027, 1558, 1820, 2041, 2176
NsiI	ATGCAT	2 cut(s)	1640, 2762
NspI	RCATGY	3 cut(s)	629, 1421, 2760
NspV	TTCGAA	1 cut(s)	497
PaeI	GCATGC	1 cut(s)	2760
PaeR7I	CTCGAG	1 cut(s)	1534
PagI	TCATGA	1 cut(s)	60
PaqCI	CACCTGC	1 cut(s)	1792
PceI	AGGCCT	1 cut(s)	1297
PciI	ACATGT	1 cut(s)	1417
PcsI	WCGNNNNNNNCGW	2 cut(s)	1689, 1698
PctI	GAATGC	4 cut(s)	188, 842, 958, 1947
PdiI	GCCGGC	1 cut(s)	179
PdmI	GAANNNNTTC	1 cut(s)	653
PfeI	GAWTC	6 cut(s)	306, 599, 649, 1196, 2107, 2332
PflFI	GACNNNGTC	1 cut(s)	2144
PflMI	CCANNNNNTGG	2 cut(s)	1078, 2781
PinAI	ACCGGT	1 cut(s)	1817
PleI	GAGTC	3 cut(s)	1030, 2088, 2643
PmaCI	CACGTG	1 cut(s)	1966
PmlI	CACGTG	1 cut(s)	1966
PpsI	GAGTC	3 cut(s)	1030, 2088, 2643
Ppu21I	YACGTR	2 cut(s)	204, 1966
PscI	ACATGT	1 cut(s)	1417
PshBI	ATTAAT	2 cut(s)	1677, 2619
Psp124BI	GAGCTC	3 cut(s)	96, 2138, 2394
Psp1406I	AACGTT	1 cut(s)	1268
Psp6I	CCWGG	2 cut(s)	1756, 1878
PspCI	CACGTG	1 cut(s)	1966
PspFI	CCCAGC	1 cut(s)	1793
PspGI	CCWGG	2 cut(s)	1756, 1878
PspN4I	GGNNCC	4 cut(s)	670, 1746, 2424, 2453
PspPI	GGNCC	2 cut(s)	1069, 2314
PspXI	VCTCGAGB	1 cut(s)	1534
PstI	CTGCAG	1 cut(s)	1513
PstNI	CAGNNNCTG	2 cut(s)	2323, 2819
PsuI	RGATCY	4 cut(s)	259, 1567, 1744, 2422
PsyI	GACNNNGTC	1 cut(s)	2144
PvuII	CAGCTG	1 cut(s)	2282
RsaI	GTAC	6 cut(s)	206, 1132, 1494, 1691, 2301, 2817
RsaNI	GTAC	6 cut(s)	205, 1131, 1493, 1690, 2300, 2816
RseI	CAYNNNNRTG	2 cut(s)	1382, 1677
SacI	GAGCTC	3 cut(s)	96, 2138, 2394
SacII	CCGCGG	1 cut(s)	960
Sau96I	GGNCC	2 cut(s)	1069, 2314
SchI	GAGTC	3 cut(s)	1030, 2088, 2644
ScrFI	CCNGG	2 cut(s)	1758, 1880
SduI	GDGCHC	5 cut(s)	96, 1148, 2138, 2394, 2490
SfaNI	GCATC	7 cut(s)	156, 366, 1201, 1537, 1625, 2053, 2670
SfcI	CTRYAG	2 cut(s)	406, 1509
Sfr274I	CTCGAG	1 cut(s)	1534
Sfr303I	CCGCGG	1 cut(s)	960
SfuI	TTCGAA	1 cut(s)	497
SgrAI	CRCCGGYG	1 cut(s)	1817
SgrBI	CCGCGG	1 cut(s)	960
SinI	GGWCC	2 cut(s)	1069, 2314
SlaI	CTCGAG	1 cut(s)	1534
SmiMI	CAYNNNNRTG	2 cut(s)	1382, 1677
SmlI	CTYRAG	1 cut(s)	1534
SmoI	CTYRAG	1 cut(s)	1534
SphI	GCATGC	1 cut(s)	2760
SseBI	AGGCCT	1 cut(s)	1297
SspI	AATATT	3 cut(s)	169, 2520, 2832
SspMI	CTAG	5 cut(s)	848, 1299, 1367, 1523, 1851
SstI	GAGCTC	3 cut(s)	96, 2138, 2394
StuI	AGGCCT	1 cut(s)	1297
StyD4I	CCNGG	2 cut(s)	1756, 1878
StyI	CCWWGG	4 cut(s)	502, 672, 1887, 2127
TaaI	ACNGT	5 cut(s)	902, 1123, 2182, 2325, 2539
TaiI	ACGT	5 cut(s)	206, 731, 1271, 1968, 2863
TatI	WGTACW	2 cut(s)	1130, 1492
TauI	GCSGC	7 cut(s)	217, 293, 487, 645, 796, 959, 2357
TfiI	GAWTC	6 cut(s)	306, 599, 649, 1196, 2107, 2332
TscAI	CASTG	5 cut(s)	142, 772, 907, 1155, 1902
TseFI	GTSAC	9 cut(s)	11, 161, 401, 758, 1027, 1558, 1820, 2041, 2176
TseI	GCWGC	8 cut(s)	285, 319, 1372, 1508, 1619, 2279, 2282, 2771
Tsp45I	GTSAC	9 cut(s)	11, 161, 401, 758, 1027, 1558, 1820, 2041, 2176
TspDTI	ATGAA	8 cut(s)	77, 734, 1067, 1181, 1212, 1344, 1596, 2797
TspGWI	ACGGA	4 cut(s)	363, 642, 2132, 2435
TspRI	CASTG	5 cut(s)	142, 772, 907, 1155, 1902
Tth111I	GACNNNGTC	1 cut(s)	2144
Van91I	CCANNNNNTGG	2 cut(s)	1078, 2781
VpaK11BI	GGWCC	2 cut(s)	1069, 2314
VspI	ATTAAT	2 cut(s)	1677, 2619
XagI	CCTNNNNNAGG	1 cut(s)	1302
XapI	RAATTY	3 cut(s)	905, 1913, 1959
XbaI	TCTAGA	1 cut(s)	1366
XceI	RCATGY	3 cut(s)	629, 1421, 2760
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1894, 2392
XhoI	CTCGAG	1 cut(s)	1534
XmnI	GAANNNNTTC	1 cut(s)	653
XspI	CTAG	5 cut(s)	848, 1299, 1367, 1523, 1851
Zsp2I	ATGCAT	2 cut(s)	1640, 2762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.