Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0000174

Physical Location & Seq

Genomic Coordinates Forward (+)

1398920 .. 1402943

Gene Structure 4024 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG007500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3090 bp

ATGTCTAGTAGTTCTGCTAGTAACTTGCATGCAAAAGTGTGGAGCATCGAGTTGATCCTAGATGTTGGAAACAAAAGTCTTAACGTGCAGAAGAGAAAGTGGCATTCTGCCTTTGTGGCCATCTACTGCTCTAGAGCCTTCCTCAAACCTCCTGTGCTAAAAGACATCAACATCAACACCAAATTTCTATCTCGCTCTCCCTCTTACACAACCATTAGTGTTGATGATTCGGTGGCTAATGGCTTCAGAATAGACCAAACAAGACTCACTGAGCTTGTGAAGGCAAAAGATTTAAAGCAGCTCCAGGAGTTGGGAGGGGTTCAAGGTGTAGGAGAAGCACTCAAAACTGATGTAGAGCATGGAATTCATGGCGAAAATGCTGAAGACATTCTCAAGAGAATCGAAGCGTTTGGTTCCAACACATACAAGAGACCACCAAAAAAAGGCTTCTTAAATTTTGTTTGGGAAGCCTTCAAAGACCTCACAATCTTAATCCTCTTGGGATGTGCTGCGCTTTCTCTTGGTTTTGGAATCAAAGAGCATGGATTAAAAGAAGGTTGGATTGACGGTGGAAGCATATTTCTTGCAATCATTCTAGTTATTTCTGTTTCGGCCATAAGCAACTATAGACAGAGCATACAATTTGACAAATTGTCAAAAGTCAGTAATAATCTTCAGATTGAGGCTGTGAGAAAGGAAAGGCGCCAACAGATTTCGATATTTGATATTGTGGTTGGTGATGTCATTTGTTTGAAGATTGGAGATCAAGTTCCAGCTGATGGATTGCTTCTGGATGGCCATTCTTTATCAGTAGATGAATCAAGCATGACAGGGGAGAGTGACCATGTTGAAATTAGCATTACTCAGAATCCATTCTTATTTTCTGGAACTAAAATTGCTGATGGTTATGGTCGGATGCTTGTCACATCTGTTGGGATGAACACAAATTGGGGTGAAATGATGAGCAAAATCAGCCAGGACACGAGTGAAAAGACGCCTCTACAAGCACGTCTAGACAAGCTAACTTCATCTATAGGTAAAGTTGGTTTGGCAGTTGCTTTCTTAGTTCTTTCAGTCATGTTGGTTCGATACTTCACAGGATATACCGTGGATGAGAATGGAAGCAAAATGTTCAATGGCAGCAAGACAAAAGTAGATGACATAATAAATGCTATCATTGGAATTGTAGCAGCTGCGGTTACAATTGTTGTAGTAGCAATTCCAGAAGGTCTGCCATTGGCTGTGACTCTCACTCTAGCTTATTCCATGAAGAGAATGATGGCTGATAATGCAATGGTACGGAAGCTCTCTGCTTGTGAGACCATGGGGTCTGCTACAACAATTTGTACAGACAAAACAGGTACTCTGACCATGAACCAGATGAAGGTGACTAAGTTTTGGTTGGGGAAAGAATCTGTGGAAGAAAAAGCTTATTCATCTATTTCTCCTTTTGTTCTCTACTTTATTCAAGAAGGGGTTGCTCTCAATACAACTGGTAGTGTGTACAGGCCTAGCTTGGATTCAAAAATTGAGATCTCTGGCAGTCCAACCGAAAAGGCTATTCTATCATGGGCGGTTCATGATACAAAGATGGATATGGAGAAAGTGGTGAAGAGTTGTAGCATTCTCCATGTTGAGGCTTTCAATTCACAGAAGAAACGTAGTGGGGTTTTGATTAAGAGGAAGGCGGATAATACAGTCCAAGTACATTTTAAAGGAGCTGCAGAGATGATACTAGCAATGTGTACAAGTTACTATGATGCCTCTGGCATTGTTAAGGATATGGATTACAATGAAAAAATGAACTTTGAGCAGATTATTCAAGGTATGGCAGCCAGCAGCCTCAGATGCATCGCATTCGCACATAAAGAAGTTGCAGCAGAGGAGCAAGTTGAAGGAGACCAGAAAGTTGTGCTAAAGGAAGATGGTTTGACCCTATTGGGACTTGTAGGTCTTAAAGATCCATGTCGTCCAGGAGTGAAGAAAGCAGTTGAAGACTGTCAATATGCAGGTGTGAACATCAAAATGATCACTGGCGATAATGTTTTCACTGCAAAAGCCATAGCTACTGAGTGTGGGATACTCAGGCCTGGTCAGGACATGCTCGGAGCAGTCATAGAAGGTGTCGAATTTCGCAACTACACTCCAGAACAGAGAATGGAGAAGGTTGAGGCAATATGTGTAATGGCGAGGTCTTCTCCTTTTGATAAGCTTCTGATGGTACAATGCTTGAAGCAGAAAGGTCATGTAGTTGCAGTGACAGGTGATGGAACCAATGATGCACCAGCATTGAAAGAAGCTGATATAGGACTTTCTATGGGGATTCAAGGCACAGAAGTTGCTAAAGAGAGCTCGGATATTGTGATCATGGATGATAACTTTGCTTCAGTTGCTACAGTTTTGATGTGGGGAAGATGTGTGTACAATAATATCCAGAAGTTCATCCAATTCCAGCTCACTGTCAATGTTGCAGCTCTTGTGATCAACTTTGTAGCCGCAGTTTCAGCAGGTCAAGTCCCATTAACAGCAGTTCAGTTACTGTGGGTAAACTTGATCATGGACACACTTGGAGCTCTTGCTCTTGCCACAGAAAAGCCCACAAAAGAACTCATGGAGAAGCCACCAGTGGGAAGGACAGCACCTCTCATCACAAATGTCATGTGGAGGAACCTCTTACCTCAAGCATTGTACCAGATAACAGTCCTCTTGATCTTGCAATTCAGGGGCAAAGTCATCTTTGGTGTCACTGATAAGGTAAAGGACACATTGATCTTCAACACTTTTGTGCTTTGCCAGGTTTTCAATGAGTTCAATGCGAGGAAGCTAGAGAAGAAGAATGTCTTCAAGGGAATACACACCAACAAGTTATTTATGGGAATCATTGCAGCAACGATTATTCTCCAAGTGGTGATGGTGGAGTTTCTTAAGAAATTTGCAGATACAGAGAGATTGAACTGGGGACAATGGGGTATATGCATTGGCATTGCAGTCATCTCTTGGCCAATTGGCTGGGTGTTCAAGTCAATACCTGTTCCAGAGAAATCCTTTCTCAGTTATCTAAAGATGAAGAATTATAAGAGCAACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1029

Amino Acids

113.08

Weight (kDa)

8.51

Isoelectric Point (pI)

29.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	104 - 173	3.8e-13	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	229 - 323	1.1e-19	P-type ATPase actuator domain
Hydrolase	PF00702	447 - 768	7.6e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	488 - 658	2.4e-28	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	839 - 1008	1.1e-47	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22910
fragaria_vesca	FvH4_4g01640 FvH4_4g01670 FvH4_4g01690 FvH4_4g01690 FvH4_4g01692 FvH4_4g06940
malus_domestica	MD04G1223800.v1.1 MD12G1240600.v1.1 MD13G1201600.v1.1 MD15G1425600.v1.1 MD15G1425900.v1.1 MD15G1426000.v1.1 MD15G1426200.v1.1 MD15G1429900.v1.1 MD16G1186200.v1.1 MD16G1201500.v1.1 MD16G1201800.v1.1 MD16G1245900.v1.1
prunus_persica	Prupe.1G023100_v2.0.a1 Prupe.1G023200_v2.0.a1 Prupe.1G023300_v2.0.a1 Prupe.1G023400_v2.0.a1 Prupe.2G086400_v2.0.a1
pyrus_communis	pycom03g20400 pycom04g03220 pycom04g03230 pycom04g19720 pycom13g17500 pycom14g13160 pycom15g37720
rosa_chinensis	RchiOBHm_Chr4g0385871 RchiOBHm_Chr4g0388251 RchiOBHm_Chr4g0388261 RchiOBHm_Chr4g0388291 RchiOBHm_Chr4g0388301 RchiOBHm_Chr4g0388321 RchiOBHm_Chr4g0388331 RchiOBHm_Chr4g0388341 RchiOBHm_Chr4g0388491 RchiOBHm_Chr4g0388501 RchiOBHm_Chr4g0388521 RchiOBHm_Chr4g0388541 RchiOBHm_Chr4g0388581 RchiOBHm_Chr4g0388591 RchiOBHm_Chr5g0038901 RchiOBHm_Chr5g0039001 RchiOBHm_Chr6g0264011
rosa_laevigata	RLG00000003378 RLG00000003379 RLG00000010036 RLG00000010037 RLG00000010039 RLG00000010041 RLG00000010042 RLG00000010043 RLG00000010049 RLG00000010050 RLG00000010051 RLG00000010052 RLG00000010054 RLG00000010055 RLG00000010062 RLG00000010064 RLG00000010065 RLG00000010066 RLG00000010067 RLG00000010069 RLG00000010070 RLG00000010071 RLG00000014295 RLG00000033880
rosa_multiflora	Rmu_co8110336.1_g000001 Rmu_sc0000533.1_g000070 Rmu_sc0000533.1_g000082 Rmu_sc0000753.1_g000013 Rmu_sc0000753.1_g000024 Rmu_sc0000753.1_g000027 Rmu_sc0000753.1_g000029 Rmu_sc0000753.1_g000030 Rmu_sc0000753.1_g000037 Rmu_sc0000753.1_g000048 Rmu_sc0001241.1_g000003 Rmu_sc0001241.1_g000020 Rmu_sc0001241.1_g000034 Rmu_sc0001241.1_g000045 Rmu_sc0001949.1_g000015 Rmu_sc0001949.1_g000020 Rmu_sc0001949.1_g000023 Rmu_sc0001949.1_g000028 Rmu_sc0002357.1_g000005 Rmu_sc0002357.1_g000017 Rmu_sc0002357.1_g000032 Rmu_sc0002357.1_g000035 Rmu_sc0002357.1_g000043 Rmu_sc0002694.1_g000002 Rmu_sc0004459.1_g000018 Rmu_sc0004773.1_g000011 Rmu_sc0006339.1_g000009 Rmu_sc0008728.1_g000008 Rmu_sc0012275.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042120 Rroxscaffold_2G00113240 Rroxscaffold_2G00131810 Rroxscaffold_4G00314870 Rroxscaffold_5G00334730 Rroxscaffold_5G00334750 Rroxscaffold_5G00334770 Rroxscaffold_5G00334790 Rroxscaffold_5G00334850 Rroxscaffold_5G00334970 Rroxscaffold_5G00335010 Rroxscaffold_5G00335030 Rroxscaffold_5G00335040
rosa_rugosa	Rorug03G0301800 Rorug03G0316600 Rorug03G0316600 Rorug03G0316700 Rorug03G0316700 Rorug03G0316800.1 Rorug03G0317300 Rorug03G0317400 Rorug03G0318000 Rorug03G0318500 Rorug03G0318600 Rorug03G0318700 Rorug03G0319000 Rorug03G0319000 Rorug03G0319100 Rorug05G0175000 Rorug05G0175500
rosa_samantha	Rh4CG007500 Rh4CG022300 Rh4CG022400 Rh4CG022600 Rh4CG022700 Rh4CG023500 Rh4CG023700 Rh4CG023900 Rh4CG024000 Rh4CG024600 Rh4CG025200 Rh4CG025300 Rh4CG025700 Rh4CG025800 Rh4CG025900 Rh4CG026000 Rh4CG026100 Rh4CG026200 Rh5BG266500 Rh6AG128500 Rh6DG110800
rosa_wichuraiana	Rw0G009370 Rw4G000360 Rw4G001430 Rw4G001440 Rw4G001460 Rw4G001470 Rw4G001490 Rw4G001500 Rw4G001520 Rw4G001620 Rw4G001630 Rw4G001650 Rw4G001670 Rw5G024450 Rw5G024490 Rw6G011090 Rw6G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3075
AarI	CACCTGC	1 cut(s)	2000
AasI	GACNNNNNNGTC	2 cut(s)	1327, 1950
Acc36I	ACCTGC	2 cut(s)	2000, 2498
AccB1I	GGYRCC	1 cut(s)	702
AccB7I	CCANNNNNTGG	2 cut(s)	310, 779
AciI	CCGC	4 cut(s)	1196, 1574, 1688, 2496
AclWI	GGATC	2 cut(s)	49, 1955
AcoI	YGGCCR	4 cut(s)	117, 612, 796, 2999
AcsI	RAATTY	5 cut(s)	182, 363, 454, 2129, 2930
AcuI	CTGAAG	4 cut(s)	229, 402, 659, 2370
AcyI	GRCGYC	2 cut(s)	703, 995
AfaI	GTAC	9 cut(s)	1299, 1348, 1363, 1505, 1707, 1747, 2223, 2423, 2690
AfiI	CCNNNNNNNGG	6 cut(s)	310, 443, 779, 1384, 1636, 2627
AflII	CTTAAG	1 cut(s)	2924
AhdI	GACNNNNNGTC	1 cut(s)	652
AjiI	CACGTC	1 cut(s)	1010
AjnI	CCWGG	5 cut(s)	303, 975, 1972, 2089, 2793
AleI	CACNNNNGTG	1 cut(s)	2783
AloI	GAACNNNNNNTCC	2 cut(s)	753, 785
Alw21I	GWGCWC	2 cut(s)	2354, 2575
Alw26I	GTCTC	3 cut(s)	424, 1313, 1893
AlwI	GGATC	2 cut(s)	49, 1955
AlwNI	CAGNNNCTG	1 cut(s)	2539
AoxI	GGCC	6 cut(s)	117, 612, 796, 1508, 2087, 2999
ApoI	RAATTY	5 cut(s)	182, 363, 454, 2129, 2930
Asp700I	GAANNNNTTC	3 cut(s)	387, 872, 2840
AspLEI	GCGC	2 cut(s)	514, 705
AsuHPI	GGTGA	6 cut(s)	749, 965, 1399, 1621, 2276, 2920
BalI	TGGCCA	3 cut(s)	119, 798, 3001
BanI	GGYRCC	1 cut(s)	702
BanII	GRGCYC	2 cut(s)	2354, 2575
BauI	CACGAG	1 cut(s)	982
BbsI	GAAGAC	4 cut(s)	390, 2001, 2187, 2833
Bbv12I	GWGCWC	2 cut(s)	2354, 2575
BcgI	CGANNNNNNTGC	2 cut(s)	1840, 1874
BciT130I	CCWGG	5 cut(s)	305, 977, 1974, 2091, 2795
BciVI	GTATCC	1 cut(s)	2073
BclI	TGATCA	4 cut(s)	2028, 2364, 2481, 2553
BcoDI	GTCTC	3 cut(s)	424, 1313, 1893
BfmI	CTRYAG	4 cut(s)	625, 1032, 1722, 2394
BfoI	RGCGCY	1 cut(s)	706
BfrI	CTTAAG	1 cut(s)	2924
BfuAI	ACCTGC	2 cut(s)	2000, 2498
BfuI	GTATCC	1 cut(s)	2073
BglI	GCCNNNNNGGC	1 cut(s)	116
BglII	AGATCT	1 cut(s)	1533
Bme1390I	CCNGG	5 cut(s)	305, 977, 1974, 2091, 2795
BmeRI	GACNNNNNGTC	1 cut(s)	652
BmgBI	CACGTC	1 cut(s)	1010
BmiI	GGNNCC	4 cut(s)	415, 704, 2272, 2669
BmrFI	CCNGG	5 cut(s)	305, 977, 1974, 2091, 2795
BmrI	ACTGGG	1 cut(s)	2965
BmsI	GCATC	6 cut(s)	54, 906, 1750, 1838, 1860, 2269
BmuI	ACTGGG	1 cut(s)	2965
BpiI	GAAGAC	4 cut(s)	390, 2001, 2187, 2833
BplI	GAGNNNNNCTC	6 cut(s)	126, 158, 324, 356, 2182, 2214
BpmI	CTGGAG	2 cut(s)	287, 2130
BpuEI	CTTGAG	2 cut(s)	377, 2664
BsaHI	GRCGYC	2 cut(s)	703, 995
BsaI	GGTCTC	3 cut(s)	424, 1313, 1893
BsaJI	CCNNGG	2 cut(s)	1107, 1323
BsaXI	ACNNNNNCTCC	2 cut(s)	753, 783
Bsc4I	CCNNNNNNNGG	6 cut(s)	310, 443, 779, 1384, 1636, 2627
Bse1I	ACTGG	4 cut(s)	1498, 2038, 2624, 2960
Bse3DI	GCAATG	4 cut(s)	1299, 1746, 2880, 2982
BseBI	CCWGG	5 cut(s)	305, 977, 1974, 2091, 2795
BseDI	CCNNGG	2 cut(s)	1107, 1323
BseGI	GGATG	7 cut(s)	509, 799, 921, 942, 1117, 2377, 2442
BseLI	CCNNNNNNNGG	6 cut(s)	310, 443, 779, 1384, 1636, 2627
BseMI	GCAATG	4 cut(s)	1299, 1746, 2880, 2982
BseMII	CTCAG	6 cut(s)	261, 878, 1858, 2061, 2098, 3064
BseNI	ACTGG	4 cut(s)	1498, 2038, 2624, 2960
BseRI	GAGGAG	1 cut(s)	1898
BseYI	CCCAGC	1 cut(s)	3009
BsgI	GTGCAG	1 cut(s)	107
BshFI	GGCC	6 cut(s)	119, 614, 798, 1510, 2089, 3001
BshNI	GGYRCC	1 cut(s)	702
BsiHKAI	GWGCWC	2 cut(s)	2354, 2575
BslFI	GGGAC	3 cut(s)	1956, 2501, 2973
BslI	CCNNNNNNNGG	6 cut(s)	310, 443, 779, 1384, 1636, 2627
BsmAI	GTCTC	3 cut(s)	424, 1313, 1893
BsmFI	GGGAC	3 cut(s)	1956, 2501, 2973
BsmI	GAATGC	3 cut(s)	103, 1623, 1856
BsnI	GGCC	6 cut(s)	119, 614, 798, 1510, 2089, 3001
Bso31I	GGTCTC	3 cut(s)	424, 1313, 1893
Bsp1286I	GDGCHC	2 cut(s)	2354, 2575
Bsp1407I	TGTACA	4 cut(s)	1346, 1503, 1745, 2421
Bsp19I	CCATGG	1 cut(s)	1323
BspACI	CCGC	4 cut(s)	1196, 1574, 1688, 2496
BspANI	GGCC	6 cut(s)	119, 614, 798, 1510, 2089, 3001
BspCNI	CTCAG	6 cut(s)	262, 877, 1857, 2062, 2097, 3063
BspHI	TCATGA	1 cut(s)	1579
BspLI	GGNNCC	4 cut(s)	415, 704, 2272, 2669
BspMAI	CTGCAG	1 cut(s)	1726
BspMI	ACCTGC	2 cut(s)	2000, 2498
BspPI	GGATC	2 cut(s)	49, 1955
BspT107I	GGYRCC	1 cut(s)	702
BspTI	CTTAAG	1 cut(s)	2924
BspTNI	GGTCTC	3 cut(s)	424, 1313, 1893
BsrDI	GCAATG	4 cut(s)	1299, 1746, 2880, 2982
BsrGI	TGTACA	4 cut(s)	1346, 1503, 1745, 2421
BsrI	ACTGG	4 cut(s)	1498, 2038, 2624, 2960
BssECI	CCNNGG	2 cut(s)	1107, 1323
BssNI	GRCGYC	2 cut(s)	703, 995
BssSI	CACGAG	1 cut(s)	982
BssT1I	CCWWGG	1 cut(s)	1323
Bst2BI	CACGAG	1 cut(s)	982
Bst2UI	CCWGG	5 cut(s)	305, 977, 1974, 2091, 2795
Bst4CI	ACNGT	8 cut(s)	569, 1108, 1699, 2000, 2398, 2461, 2541, 2701
Bst6I	CTCTTC	3 cut(s)	86, 1265, 1607
BstACI	GRCGYC	2 cut(s)	703, 995
BstAFI	CTTAAG	1 cut(s)	2924
BstAUI	TGTACA	4 cut(s)	1346, 1503, 1745, 2421
BstC8I	GCNNGC	2 cut(s)	30, 1837
BstDEI	CTNAG	8 cut(s)	270, 864, 1063, 1392, 1844, 2070, 2084, 3050
BstDSI	CCRYGG	2 cut(s)	1107, 1323
BstF5I	GGATG	7 cut(s)	509, 799, 921, 942, 1117, 2377, 2442
BstH2I	RGCGCY	1 cut(s)	706
BstHHI	GCGC	2 cut(s)	514, 705
BstMAI	GTCTC	3 cut(s)	424, 1313, 1893
BstMWI	GCNNNNNNNGC	6 cut(s)	116, 972, 1289, 1848, 2390, 2504
BstNI	CCWGG	5 cut(s)	305, 977, 1974, 2091, 2795
BstNSI	RCATGY	2 cut(s)	32, 2104
BstSCI	CCNGG	5 cut(s)	303, 975, 1972, 2089, 2793
BstSFI	CTRYAG	4 cut(s)	625, 1032, 1722, 2394
BstV2I	GAAGAC	4 cut(s)	390, 2001, 2187, 2833
BstX2I	RGATCY	2 cut(s)	1533, 1960
BstXI	CCANNNNNNTGG	1 cut(s)	3009
BstYI	RGATCY	2 cut(s)	1533, 1960
BsuI	GTATCC	1 cut(s)	2073
BsuRI	GGCC	6 cut(s)	119, 614, 798, 1510, 2089, 3001
BtgI	CCRYGG	2 cut(s)	1107, 1323
BtgZI	GCGATG	1 cut(s)	1837
BtrI	CACGTC	1 cut(s)	1010
BtsCI	GGATG	7 cut(s)	509, 799, 921, 942, 1117, 2377, 2442
BtsI	GCAGTG	2 cut(s)	2049, 2262
BtsIMutI	CAGTG	7 cut(s)	267, 2031, 2049, 2262, 2457, 2631, 2745
BveI	ACCTGC	2 cut(s)	2000, 2498
Cac8I	GCNNGC	2 cut(s)	30, 1837
CaiI	CAGNNNCTG	1 cut(s)	2539
CciI	TCATGA	1 cut(s)	1579
CfoI	GCGC	2 cut(s)	514, 705
CseI	GACGC	1 cut(s)	1003
Csp6I	GTAC	9 cut(s)	1298, 1347, 1362, 1504, 1706, 1746, 2222, 2422, 2689
CviQI	GTAC	9 cut(s)	1298, 1347, 1362, 1504, 1706, 1746, 2222, 2422, 2689
DdeI	CTNAG	8 cut(s)	270, 864, 1063, 1392, 1844, 2070, 2084, 3050
DinI	GGCGCC	1 cut(s)	704
DraI	TTTAAA	2 cut(s)	294, 1714
DrdI	GACNNNNNNGTC	2 cut(s)	1327, 1950
DriI	GACNNNNNGTC	1 cut(s)	652
DseDI	GACNNNNNNGTC	2 cut(s)	1327, 1950
EaeI	YGGCCR	4 cut(s)	117, 612, 796, 2999
Eam1104I	CTCTTC	3 cut(s)	86, 1265, 1607
Eam1105I	GACNNNNNGTC	1 cut(s)	652
EarI	CTCTTC	3 cut(s)	86, 1265, 1607
EciI	GGCGGA	1 cut(s)	1703
Ecl136II	GAGCTC	2 cut(s)	2352, 2573
Eco130I	CCWWGG	1 cut(s)	1323
Eco147I	AGGCCT	2 cut(s)	1510, 2089
Eco24I	GRGCYC	2 cut(s)	2354, 2575
Eco31I	GGTCTC	3 cut(s)	424, 1313, 1893
Eco53kI	GAGCTC	2 cut(s)	2352, 2573
Eco57I	CTGAAG	4 cut(s)	229, 402, 659, 2370
EcoICRI	GAGCTC	2 cut(s)	2352, 2573
EcoRI	GAATTC	1 cut(s)	363
EcoRII	CCWGG	5 cut(s)	303, 975, 1972, 2089, 2793
EcoT14I	CCWWGG	1 cut(s)	1323
EcoT22I	ATGCAT	2 cut(s)	1853, 2978
EcoT38I	GRGCYC	2 cut(s)	2354, 2575
EgeI	GGCGCC	1 cut(s)	704
EheI	GGCGCC	1 cut(s)	704
ErhI	CCWWGG	1 cut(s)	1323
FalI	AAGNNNNNCTT	2 cut(s)	2825, 2857
FaqI	GGGAC	3 cut(s)	1956, 2501, 2973
FbaI	TGATCA	4 cut(s)	2028, 2364, 2481, 2553
FokI	GGATG	7 cut(s)	516, 806, 928, 949, 1124, 2384, 2429
FriOI	GRGCYC	2 cut(s)	2354, 2575
GlaI	GCGC	2 cut(s)	513, 704
GsaI	CCCAGC	1 cut(s)	3013
GsuI	CTGGAG	2 cut(s)	287, 2130
HaeII	RGCGCY	1 cut(s)	706
HaeIII	GGCC	6 cut(s)	119, 614, 798, 1510, 2089, 3001
HgaI	GACGC	1 cut(s)	1003
HhaI	GCGC	2 cut(s)	514, 705
Hin1I	GRCGYC	2 cut(s)	703, 995
Hin6I	GCGC	2 cut(s)	512, 703
HinP1I	GCGC	2 cut(s)	512, 703
HindIII	AAGCTT	2 cut(s)	1428, 2210
HphI	GGTGA	6 cut(s)	749, 965, 1399, 1621, 2276, 2920
Hpy166II	GTNNAC	5 cut(s)	1504, 1746, 2017, 2422, 2548
Hpy188I	TCNGA	9 cut(s)	248, 678, 867, 915, 1368, 1847, 2108, 2217, 2356
Hpy8I	GTNNAC	5 cut(s)	1504, 1746, 2017, 2422, 2548
HpyCH4III	ACNGT	8 cut(s)	569, 1108, 1699, 2000, 2398, 2461, 2541, 2701
HpyCH4IV	ACGT	3 cut(s)	84, 1009, 1660
HpyF10VI	GCNNNNNNNGC	6 cut(s)	116, 972, 1289, 1848, 2390, 2504
HpyF3I	CTNAG	8 cut(s)	270, 864, 1063, 1392, 1844, 2070, 2084, 3050
HpySE526I	ACGT	3 cut(s)	84, 1009, 1660
Hsp92I	GRCGYC	2 cut(s)	703, 995
HspAI	GCGC	2 cut(s)	512, 703
KasI	GGCGCC	1 cut(s)	702
Ksp22I	TGATCA	4 cut(s)	2028, 2364, 2481, 2553
LmnI	GCTCC	6 cut(s)	42, 306, 1718, 1885, 2108, 2570
LweI	GCATC	6 cut(s)	54, 906, 1750, 1838, 1860, 2269
MaeII	ACGT	3 cut(s)	84, 1009, 1660
MfeI	CAATTG	2 cut(s)	1203, 3003
MflI	RGATCY	2 cut(s)	1533, 1960
MhlI	GDGCHC	2 cut(s)	2354, 2575
MlsI	TGGCCA	3 cut(s)	119, 798, 3001
MluNI	TGGCCA	3 cut(s)	119, 798, 3001
Mly113I	GGCGCC	1 cut(s)	703
MlyI	GAGTC	2 cut(s)	258, 1240
MmeI	TCCRAC	5 cut(s)	46, 441, 539, 893, 1571
Mox20I	TGGCCA	3 cut(s)	119, 798, 3001
Mph1103I	ATGCAT	2 cut(s)	1853, 2978
MroXI	GAANNNNTTC	3 cut(s)	387, 872, 2840
MscI	TGGCCA	3 cut(s)	119, 798, 3001
MslI	CAYNNNNRTG	2 cut(s)	2024, 2783
Msp20I	TGGCCA	3 cut(s)	119, 798, 3001
MspA1I	CMGCKG	2 cut(s)	776, 1193
MspCI	CTTAAG	1 cut(s)	2924
MspR9I	CCNGG	5 cut(s)	305, 977, 1974, 2091, 2795
MunI	CAATTG	2 cut(s)	1203, 3003
Mva1269I	GAATGC	3 cut(s)	103, 1623, 1856
MvaI	CCWGG	5 cut(s)	305, 977, 1974, 2091, 2795
MwoI	GCNNNNNNNGC	6 cut(s)	116, 972, 1289, 1848, 2390, 2504
NarI	GGCGCC	1 cut(s)	703
NcoI	CCATGG	1 cut(s)	1323
NlaIV	GGNNCC	4 cut(s)	415, 704, 2272, 2669
NmuCI	GTSAC	6 cut(s)	839, 922, 1243, 1387, 2257, 2743
NsiI	ATGCAT	2 cut(s)	1853, 2978
NspI	RCATGY	2 cut(s)	32, 2104
OliI	CACNNNNGTG	1 cut(s)	2783
PaeI	GCATGC	1 cut(s)	32
PagI	TCATGA	1 cut(s)	1579
PaqCI	CACCTGC	1 cut(s)	2000
PceI	AGGCCT	2 cut(s)	1510, 2089
PctI	GAATGC	3 cut(s)	103, 1623, 1856
PdmI	GAANNNNTTC	3 cut(s)	387, 872, 2840
PfeI	GAWTC	9 cut(s)	227, 399, 531, 818, 868, 1412, 1520, 2323, 2877
PflMI	CCANNNNNTGG	2 cut(s)	310, 779
PfoI	TCCNGGA	2 cut(s)	303, 1972
PleI	GAGTC	2 cut(s)	258, 1240
PluTI	GGCGCC	1 cut(s)	706
PpsI	GAGTC	2 cut(s)	258, 1240
PsiI	TTATAA	1 cut(s)	3075
Psp124BI	GAGCTC	2 cut(s)	2354, 2575
Psp6I	CCWGG	5 cut(s)	303, 975, 1972, 2089, 2793
PspFI	CCCAGC	1 cut(s)	3009
PspGI	CCWGG	5 cut(s)	303, 975, 1972, 2089, 2793
PspN4I	GGNNCC	4 cut(s)	415, 704, 2272, 2669
PstI	CTGCAG	1 cut(s)	1726
PstNI	CAGNNNCTG	1 cut(s)	2539
PsuI	RGATCY	2 cut(s)	1533, 1960
PvuII	CAGCTG	2 cut(s)	776, 1193
RsaI	GTAC	9 cut(s)	1299, 1348, 1363, 1505, 1707, 1747, 2223, 2423, 2690
RsaNI	GTAC	9 cut(s)	1298, 1347, 1362, 1504, 1706, 1746, 2222, 2422, 2689
RseI	CAYNNNNRTG	2 cut(s)	2024, 2783
SacI	GAGCTC	2 cut(s)	2354, 2575
SchI	GAGTC	2 cut(s)	258, 1240
ScrFI	CCNGG	5 cut(s)	305, 977, 1974, 2091, 2795
SduI	GDGCHC	2 cut(s)	2354, 2575
SfaNI	GCATC	6 cut(s)	54, 906, 1750, 1838, 1860, 2269
SfcI	CTRYAG	4 cut(s)	625, 1032, 1722, 2394
SfoI	GGCGCC	1 cut(s)	704
SmiMI	CAYNNNNRTG	2 cut(s)	2024, 2783
SmlI	CTYRAG	3 cut(s)	392, 2679, 2924
SmoI	CTYRAG	3 cut(s)	392, 2679, 2924
SphI	GCATGC	1 cut(s)	32
SseBI	AGGCCT	2 cut(s)	1510, 2089
SsiI	CCGC	4 cut(s)	1196, 1574, 1688, 2496
SspDI	GGCGCC	1 cut(s)	702
SstI	GAGCTC	2 cut(s)	2354, 2575
StuI	AGGCCT	2 cut(s)	1510, 2089
StyD4I	CCNGG	5 cut(s)	303, 975, 1972, 2089, 2793
StyI	CCWWGG	1 cut(s)	1323
TaaI	ACNGT	8 cut(s)	569, 1108, 1699, 2000, 2398, 2461, 2541, 2701
TaiI	ACGT	3 cut(s)	87, 1012, 1663
TaqI	TCGA	5 cut(s)	48, 402, 716, 1087, 2127
TatI	WGTACW	5 cut(s)	1346, 1503, 1705, 1745, 2421
TauI	GCSGC	1 cut(s)	2498
TfiI	GAWTC	9 cut(s)	227, 399, 531, 818, 868, 1412, 1520, 2323, 2877
TscAI	CASTG	7 cut(s)	274, 2038, 2056, 2262, 2464, 2631, 2752
TseFI	GTSAC	6 cut(s)	839, 922, 1243, 1387, 2257, 2743
Tsp45I	GTSAC	6 cut(s)	839, 922, 1243, 1387, 2257, 2743
TspGWI	ACGGA	1 cut(s)	1315
TspRI	CASTG	7 cut(s)	274, 2038, 2056, 2262, 2464, 2631, 2752
Van91I	CCANNNNNTGG	2 cut(s)	310, 779
Vha464I	CTTAAG	1 cut(s)	2924
XapI	RAATTY	5 cut(s)	182, 363, 454, 2129, 2930
XbaI	TCTAGA	2 cut(s)	131, 1012
XceI	RCATGY	2 cut(s)	32, 2104
XmnI	GAANNNNTTC	3 cut(s)	387, 872, 2840
Zsp2I	ATGCAT	2 cut(s)	1853, 2978

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.