Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0000174

Physical Location & Seq

Genomic Coordinates Forward (+)

2012488 .. 2015793

Gene Structure 3306 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00334730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3015 bp

ATGACGTCTCTGCATGATCCTGTCGACCTCTGGGAAGTTTCAGACATAATTTCTGCTGTGCCTGTTGAAAGGGTTTTGGCTGTTATGGAAAGTGCTCAGAATATTACTAATGAGGAAATGATAAGCCTTCCAGTCATTCTTCCAGTCAGTGTCAGACAACAACGGTTTCAAACTAGACCAAAAACCCTCACCCAGCTTGTGAAGGATAAAAGTCTTGATCAGCTCCAGGAGTTGGGAGGGGTTGAAGGAGTAGCATCAGCCCTCAAAACTGATGCAGCGCATGGAGTTTGCGGTGACGCTGAAGATGTTGCACACCGACTTGAAGCATTTGGTTTGAATACATACAAGATAGCAGCAGCCAAGAGGTTCTTCCATTTTGTGCGGAAAGCATGCCAAGACCTCACAATTTTTATTCTTATAGGCTGTGCTGCACTCTCTCTTGCTTTGGGCATCAAAGAGCATGGATTAAGAGAAGGTTGGAGTGATGGTGGAAGCATATTTCTTGCAGTCATTCTAGTGATTTCAGTGTCTGCCATAAGCAATTACAGGCAGCACAGAGAGTTTGACAAATTGTCAAAAGTCGGTAGCAATCTCCAAATTGAAGCTGTGAGAAATGGCAAGAGACAACATATTTTAATATTTGAAATTGTGGTTGGAGATGTCATTTGCTTAAAAATTGGAGATCAAGTTCCAGCCGACGGGTTGCTCTTGGAGGGATATTCATTGGAGGTAGATGAATCAAGCATGACAGGGGAGAGTGACCATGTCGAAATCAACCAAAGCCAGAACCCATTCTTGTTTTCTGGCACCAAAGTGGTTAACGGGCATGCTCAGATGCTTGTCACTTCCGTCGGCATGAACACAGCATGGGGTGCAATGATGAGCCAAATCAGCAGTGATACAAGTGAACAGACACCTTTACAAGCTCGCATCCATGGGCTAGCCTCATCAATAGGTATAGTTGGTTTGCTAGTAGCTTTGTCAAATCTCATAGTCTCAAAGATATACTACTTCACCGGAAATACCAAGGACAAACATGGCAAGAGGGAGTTCAATGGGAGCAGAACCAAAGTTAATCGCATGATAATGGCCATCATTGGGATGGTAGGAACAGCAGTTACAATTGTTGTGGCTGCAATTCCAGAAGGTTTACCAGTTGCCATGACGATTACTCTCTGTTATGCAATGAAGAGAATGATGGCTGATAAAGCAATGGTTAGGAAACTCTCTGCTTGTGAGATTATGGGCTCTGCTACCACCATTTGCACTGACAAAACAGGAACTCTCACGATGAACCAGATGAAGGTGGCCAAGTTCTGGATAGGTGAAAAGCCCGTGGAAGAAGTCTACTCATCAATTTCGTCCCATCTTCTTAGCTTGATTCGAGAAGGCGTTGCTATGAACACAACAGGTTCTAGGCACATTTCAGGATCTGGATTTGAATTTTCAGGCAGTCCTACTGAAAAAGCCATTCTTTCATGGGCATCTTTCGACCTGCATATGGACATGGAGAGATTGAAAAACAATTCTATCATTCAAGATGTTGAAGCCTTCAATTCACACAAGAAAAGAAGTGGTATTTTGATCAAGAGGAAGATAGACAACAGTCACAACACAATGCAAGTACATTGGAAAGGAGCTGCGGAGGTGATACTAGCAATGTGCAACAGTTACCACAATGCCTCTGGACATATAAAAGATATGGATGACGATGCAAAGGGAAAACTTGAGCATATTATTCAAGGTATGGCAGCTAGTAGCCTCCGATGCATTGCATTTGCACACAAAGAAGTATCTGCAGAGGAGCAAGTGGATCCAGAACATCATAAGACTTTGCTAAAAGACAATGGCTTGACCCTATTAGGACTTGTGGGTCTTAAAGATCCATGTCGGCCAGGAGTGAAGAAAGCTGTTGAGGACTGTCAACATGCAGGGGTGAACATCAAAATGATCACAGGTGACAACATCTTTACTGCAAAATCGATAGCCACGGAATGTGGGATACTGAAGTCTGAGCAGGACACGATCAGTGAAGCTGTGATAGAGGGTGTGCAATTTCGGAACTACACACCAGAAGAGAGAATGCAGAAAGTAGACAATATCCGTGTCATGGCAAGGTCATCCCCTTTTGATAAGCTTCTTATGGTGCAATGCTTGAAGCAGAAAGGTCATGTAGTTGCAGTGACTGGTGATGGCGCAAATGATGCTCCTGCATTGAAAGAAGCTGACGTAGGTCTCTCTATGGGGATGCAAGGCACTGAAGTTGCGAAAGATAGCTCAGACATTGTGATCATGGATGACGATTTTGCTTCAGTAGCCACAGTTTTGATGTGGGGAAGATGTGTGTACAATAACATCCAAAAGTTCATTCAATTCCAACTCACTGTCAATGTTGCAGCTCTTGTAATCAACTTTGTGGCAGCAGTTTCAGCAAGTAAGGTGCCCTTAACAGCAGTTCAATTATTGTGGGTGAACTTGATTATGGACACACTGGGTGCTCTGGCCCTTGCGACAGACAAACCCACAAAAGAACTCATGGAGAATCCACCTGTGAGAAGAACGGAGCCTCTCATCACCAACATCATGTGGAGGAACCTTTTACCTCAAGCTCTGTACCAGATAGCAGTAGTATTGACCTTACGATTCAGGGGCAACACAATCTTTGGTGTAGATGATAAGGTACAAGATACCTTGATCTTTAACACGTTTGTTCTCTGCCAGGTCTTCAATGAATTCAACGCAAGAAAGCTTGAGAAAAAGAACGTTTTCAAGGGACTGTTCACCAACAAAATCTTTTTGGTGATCATTGCATTAATAATTATTCTGCAGCTTGTGATGGTGGAACTTCTGAACAAATACGCAGATACAGAGAGGTTGAATTGGGGGCAATGGGGTGTATGTCTTGGAATTGCGGCCATGTCTTGGCCACTCGGGTGGGTTGTTAAGTGTCTACCTGTTCCACAGAAACCTATCGTCAGCTATCTGGACGTGAAGATATACAAGAACTCCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1004

Amino Acids

110.29

Weight (kDa)

6.94

Isoelectric Point (pI)

32.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	78 - 146	4.3e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	202 - 297	6.7e-22	P-type ATPase actuator domain
Hydrolase	PF00702	420 - 743	1.1e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	459 - 632	8e-27	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	814 - 983	1.6e-48	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22910
fragaria_vesca	FvH4_4g01640 FvH4_4g01670 FvH4_4g01690 FvH4_4g01690 FvH4_4g01692 FvH4_4g06940
malus_domestica	MD04G1223800.v1.1 MD12G1240600.v1.1 MD13G1201600.v1.1 MD15G1425600.v1.1 MD15G1425900.v1.1 MD15G1426000.v1.1 MD15G1426200.v1.1 MD15G1429900.v1.1 MD16G1186200.v1.1 MD16G1201500.v1.1 MD16G1201800.v1.1 MD16G1245900.v1.1
prunus_persica	Prupe.1G023100_v2.0.a1 Prupe.1G023200_v2.0.a1 Prupe.1G023300_v2.0.a1 Prupe.1G023400_v2.0.a1 Prupe.2G086400_v2.0.a1
pyrus_communis	pycom03g20400 pycom04g03220 pycom04g03230 pycom04g19720 pycom13g17500 pycom14g13160 pycom15g37720
rosa_chinensis	RchiOBHm_Chr4g0385871 RchiOBHm_Chr4g0388251 RchiOBHm_Chr4g0388261 RchiOBHm_Chr4g0388291 RchiOBHm_Chr4g0388301 RchiOBHm_Chr4g0388321 RchiOBHm_Chr4g0388331 RchiOBHm_Chr4g0388341 RchiOBHm_Chr4g0388491 RchiOBHm_Chr4g0388501 RchiOBHm_Chr4g0388521 RchiOBHm_Chr4g0388541 RchiOBHm_Chr4g0388581 RchiOBHm_Chr4g0388591 RchiOBHm_Chr5g0038901 RchiOBHm_Chr5g0039001 RchiOBHm_Chr6g0264011
rosa_laevigata	RLG00000003378 RLG00000003379 RLG00000010036 RLG00000010037 RLG00000010039 RLG00000010041 RLG00000010042 RLG00000010043 RLG00000010049 RLG00000010050 RLG00000010051 RLG00000010052 RLG00000010054 RLG00000010055 RLG00000010062 RLG00000010064 RLG00000010065 RLG00000010066 RLG00000010067 RLG00000010069 RLG00000010070 RLG00000010071 RLG00000014295 RLG00000033880
rosa_multiflora	Rmu_co8110336.1_g000001 Rmu_sc0000533.1_g000070 Rmu_sc0000533.1_g000082 Rmu_sc0000753.1_g000013 Rmu_sc0000753.1_g000024 Rmu_sc0000753.1_g000027 Rmu_sc0000753.1_g000029 Rmu_sc0000753.1_g000030 Rmu_sc0000753.1_g000037 Rmu_sc0000753.1_g000048 Rmu_sc0001241.1_g000003 Rmu_sc0001241.1_g000020 Rmu_sc0001241.1_g000034 Rmu_sc0001241.1_g000045 Rmu_sc0001949.1_g000015 Rmu_sc0001949.1_g000020 Rmu_sc0001949.1_g000023 Rmu_sc0001949.1_g000028 Rmu_sc0002357.1_g000005 Rmu_sc0002357.1_g000017 Rmu_sc0002357.1_g000032 Rmu_sc0002357.1_g000035 Rmu_sc0002357.1_g000043 Rmu_sc0002694.1_g000002 Rmu_sc0004459.1_g000018 Rmu_sc0004773.1_g000011 Rmu_sc0006339.1_g000009 Rmu_sc0008728.1_g000008 Rmu_sc0012275.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042120 Rroxscaffold_2G00113240 Rroxscaffold_2G00131810 Rroxscaffold_4G00314870 Rroxscaffold_5G00334730 Rroxscaffold_5G00334750 Rroxscaffold_5G00334770 Rroxscaffold_5G00334790 Rroxscaffold_5G00334850 Rroxscaffold_5G00334970 Rroxscaffold_5G00335010 Rroxscaffold_5G00335030 Rroxscaffold_5G00335040
rosa_rugosa	Rorug03G0301800 Rorug03G0316600 Rorug03G0316600 Rorug03G0316700 Rorug03G0316700 Rorug03G0316800.1 Rorug03G0317300 Rorug03G0317400 Rorug03G0318000 Rorug03G0318500 Rorug03G0318600 Rorug03G0318700 Rorug03G0319000 Rorug03G0319000 Rorug03G0319100 Rorug05G0175000 Rorug05G0175500
rosa_samantha	Rh4CG007500 Rh4CG022300 Rh4CG022400 Rh4CG022600 Rh4CG022700 Rh4CG023500 Rh4CG023700 Rh4CG023900 Rh4CG024000 Rh4CG024600 Rh4CG025200 Rh4CG025300 Rh4CG025700 Rh4CG025800 Rh4CG025900 Rh4CG026000 Rh4CG026100 Rh4CG026200 Rh5BG266500 Rh6AG128500 Rh6DG110800
rosa_wichuraiana	Rw0G009370 Rw4G000360 Rw4G001430 Rw4G001440 Rw4G001460 Rw4G001470 Rw4G001490 Rw4G001500 Rw4G001520 Rw4G001620 Rw4G001630 Rw4G001650 Rw4G001670 Rw5G024450 Rw5G024490 Rw6G011090 Rw6G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1872
AatII	GACGTC	1 cut(s)	8
Acc36I	ACCTGC	1 cut(s)	1503
AccB1I	GGYRCC	2 cut(s)	806, 2440
AccB7I	CCANNNNNTGG	3 cut(s)	232, 1317, 2922
AccI	GTMKAC	4 cut(s)	24, 1347, 2094, 2950
AciI	CCGC	4 cut(s)	291, 382, 1643, 2912
AclI	AACGTT	1 cut(s)	2763
AclWI	GGATC	5 cut(s)	11, 1438, 1808, 1821, 1877
AcoI	YGGCCR	5 cut(s)	1089, 1308, 1892, 2913, 2924
AcsI	RAATTY	2 cut(s)	1442, 2732
AcuI	CTGAAG	4 cut(s)	321, 2027, 2280, 2295
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	1 cut(s)	2495
AfaI	GTAC	4 cut(s)	1626, 2348, 2615, 2682
AfiI	CCNNNNNNNGG	8 cut(s)	232, 698, 1098, 1303, 1317, 1501, 2110, 2922
AflIII	ACRYGT	1 cut(s)	2703
AhdI	GACNNNNNGTC	1 cut(s)	571
AjiI	CACGTC	1 cut(s)	2989
AjnI	CCWGG	3 cut(s)	225, 1894, 2718
AjuI	GAANNNNNNNTTGG	6 cut(s)	353, 385, 636, 668, 2352, 2384
AleI	CACNNNNGTG	2 cut(s)	812, 2932
AloI	GAACNNNNNNTCC	2 cut(s)	672, 704
Alw21I	GWGCWC	2 cut(s)	97, 2500
Alw26I	GTCTC	4 cut(s)	12, 616, 1000, 2240
AlwI	GGATC	5 cut(s)	11, 1438, 1808, 1821, 1877
AlwNI	CAGNNNCTG	3 cut(s)	530, 1433, 2186
Ama87I	CYCGRG	1 cut(s)	2930
AoxI	GGCC	6 cut(s)	1089, 1308, 1892, 2502, 2913, 2924
ApoI	RAATTY	2 cut(s)	1442, 2732
AseI	ATTAAT	1 cut(s)	2813
Asp700I	GAANNNNTTC	2 cut(s)	791, 2765
AspLEI	GCGC	2 cut(s)	280, 2198
AspS9I	GGNCC	1 cut(s)	2503
AsuNHI	GCTAGC	1 cut(s)	940
AvaI	CYCGRG	1 cut(s)	2930
BaeGI	GKGCMC	1 cut(s)	2445
BaeI	ACNNNNGTAYC	2 cut(s)	1994, 2027
BalI	TGGCCA	3 cut(s)	1091, 1310, 2926
BamHI	GGATCC	1 cut(s)	1813
BanI	GGYRCC	2 cut(s)	806, 2440
BanII	GRGCYC	1 cut(s)	1250
BbsI	GAAGAC	1 cut(s)	2716
Bbv12I	GWGCWC	2 cut(s)	97, 2500
BccI	CCATC	7 cut(s)	479, 1096, 1100, 1192, 1374, 2186, 2830
BciT130I	CCWGG	3 cut(s)	227, 1896, 2720
BciVI	GTATCC	1 cut(s)	1995
BclI	TGATCA	5 cut(s)	217, 1584, 1950, 2289, 2802
BcoDI	GTCTC	4 cut(s)	12, 616, 1000, 2240
BfaI	CTAG	7 cut(s)	174, 515, 941, 971, 1416, 1655, 1755
BfmI	CTRYAG	2 cut(s)	1797, 2825
BfuAI	ACCTGC	1 cut(s)	1503
BfuI	GTATCC	1 cut(s)	1995
Bme1390I	CCNGG	3 cut(s)	227, 1896, 2720
BmeRI	GACNNNNNGTC	1 cut(s)	571
BmeT110I	CYCGRG	1 cut(s)	2930
BmgBI	CACGTC	1 cut(s)	2989
BmgT120I	GGNCC	1 cut(s)	2503
BmiI	GGNNCC	5 cut(s)	808, 1815, 2442, 2565, 2594
BmrFI	CCNGG	3 cut(s)	227, 1896, 2720
BmrI	ACTGGG	1 cut(s)	2501
BmtI	GCTAGC	1 cut(s)	944
BmuI	ACTGGG	1 cut(s)	2501
BoxI	GACNNNNGTC	2 cut(s)	1605, 2232
BpiI	GAAGAC	1 cut(s)	2716
BpmI	CTGGAG	2 cut(s)	209, 2992
BpuEI	CTTGAG	3 cut(s)	1748, 2589, 2771
Bsa29I	ATCGAT	1 cut(s)	1982
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	2240
BsaJI	CCNNGG	4 cut(s)	934, 1026, 1335, 1989
BsaWI	WCCGGW	1 cut(s)	1016
BsaXI	ACNNNNNCTCC	5 cut(s)	472, 502, 672, 702, 2990
Bsc4I	CCNNNNNNNGG	8 cut(s)	232, 698, 1098, 1303, 1317, 1501, 2110, 2922
Bse1I	ACTGG	6 cut(s)	131, 143, 1154, 2191, 2496, 3009
Bse3DI	GCAATG	8 cut(s)	882, 1191, 1218, 1665, 1770, 2156, 2805, 2894
BseBI	CCWGG	3 cut(s)	227, 1896, 2720
BseCI	ATCGAT	1 cut(s)	1982
BseDI	CCNNGG	4 cut(s)	934, 1026, 1335, 1989
BseGI	GGATG	7 cut(s)	930, 1107, 1711, 2120, 2253, 2302, 2355
BseLI	CCNNNNNNNGG	8 cut(s)	232, 698, 1098, 1303, 1317, 1501, 2110, 2922
BseMI	GCAATG	8 cut(s)	882, 1191, 1218, 1665, 1770, 2156, 2805, 2894
BseMII	CTCAG	4 cut(s)	110, 845, 2004, 2292
BseNI	ACTGG	6 cut(s)	131, 143, 1154, 2191, 2496, 3009
BseRI	GAGGAG	1 cut(s)	1817
BseSI	GKGCMC	1 cut(s)	2445
BseYI	CCCAGC	1 cut(s)	192
BsgI	GTGCAG	1 cut(s)	414
BshFI	GGCC	6 cut(s)	1091, 1310, 1894, 2504, 2915, 2926
BshNI	GGYRCC	2 cut(s)	806, 2440
BshVI	ATCGAT	1 cut(s)	1982
BsiHKAI	GWGCWC	2 cut(s)	97, 2500
BsiHKCI	CYCGRG	1 cut(s)	2930
BsiSI	CCGG	1 cut(s)	1017
BslFI	GGGAC	2 cut(s)	1348, 2787
BslI	CCNNNNNNNGG	8 cut(s)	232, 698, 1098, 1303, 1317, 1501, 2110, 2922
BsmAI	GTCTC	4 cut(s)	12, 616, 1000, 2240
BsmBI	CGTCTC	1 cut(s)	12
BsmFI	GGGAC	2 cut(s)	1348, 2787
BsmI	GAATGC	1 cut(s)	2088
BsnI	GGCC	6 cut(s)	1091, 1310, 1894, 2504, 2915, 2926
Bso31I	GGTCTC	1 cut(s)	2240
BsoBI	CYCGRG	1 cut(s)	2930
Bsp1286I	GDGCHC	4 cut(s)	97, 1250, 2445, 2500
Bsp1407I	TGTACA	1 cut(s)	2346
Bsp19I	CCATGG	1 cut(s)	934
BspACI	CCGC	4 cut(s)	291, 382, 1643, 2912
BspANI	GGCC	6 cut(s)	1091, 1310, 1894, 2504, 2915, 2926
BspCNI	CTCAG	4 cut(s)	109, 844, 2005, 2291
BspDI	ATCGAT	1 cut(s)	1982
BspLI	GGNNCC	5 cut(s)	808, 1815, 2442, 2565, 2594
BspMAI	CTGCAG	2 cut(s)	1801, 2829
BspMI	ACCTGC	1 cut(s)	1503
BspOI	GCTAGC	1 cut(s)	944
BspPI	GGATC	5 cut(s)	11, 1438, 1808, 1821, 1877
BspT107I	GGYRCC	2 cut(s)	806, 2440
BspTNI	GGTCTC	1 cut(s)	2240
BsrDI	GCAATG	8 cut(s)	882, 1191, 1218, 1665, 1770, 2156, 2805, 2894
BsrGI	TGTACA	1 cut(s)	2346
BsrI	ACTGG	6 cut(s)	131, 143, 1154, 2191, 2496, 3009
BssECI	CCNNGG	4 cut(s)	934, 1026, 1335, 1989
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	934, 1026
Bst2UI	CCWGG	3 cut(s)	227, 1896, 2720
Bst4CI	ACNGT	7 cut(s)	165, 1607, 1670, 1922, 2323, 2386, 2778
Bst6I	CTCTTC	2 cut(s)	1184, 2070
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	2 cut(s)	872, 2204
BstAUI	TGTACA	1 cut(s)	2346
BstC8I	GCNNGC	4 cut(s)	391, 828, 928, 942
BstDEI	CTNAG	5 cut(s)	96, 831, 1373, 2013, 2278
BstDSI	CCRYGG	3 cut(s)	934, 1335, 1989
BstF5I	GGATG	7 cut(s)	930, 1107, 1711, 2120, 2253, 2302, 2355
BstHHI	GCGC	2 cut(s)	280, 2198
BstMAI	GTCTC	4 cut(s)	12, 616, 1000, 2240
BstMWI	GCNNNNNNNGC	7 cut(s)	872, 891, 1208, 1767, 2204, 2315, 2429
BstNI	CCWGG	3 cut(s)	227, 1896, 2720
BstNSI	RCATGY	3 cut(s)	393, 830, 1931
BstPAI	GACNNNNGTC	2 cut(s)	1605, 2232
BstSCI	CCNGG	3 cut(s)	225, 1894, 2718
BstSFI	CTRYAG	2 cut(s)	1797, 2825
BstSLI	GKGCMC	1 cut(s)	2445
BstV2I	GAAGAC	1 cut(s)	2716
BstX2I	RGATCY	3 cut(s)	1430, 1813, 1882
BstXI	CCANNNNNNTGG	1 cut(s)	2934
BstYI	RGATCY	3 cut(s)	1430, 1813, 1882
Bsu15I	ATCGAT	1 cut(s)	1982
BsuI	GTATCC	1 cut(s)	1995
BsuRI	GGCC	6 cut(s)	1091, 1310, 1894, 2504, 2915, 2926
BsuTUI	ATCGAT	1 cut(s)	1982
BtgI	CCRYGG	3 cut(s)	934, 1335, 1989
BtrI	CACGTC	1 cut(s)	2989
BtsCI	GGATG	7 cut(s)	930, 1107, 1711, 2120, 2253, 2302, 2355
BtsI	GCAGTG	2 cut(s)	901, 2187
BtsIMutI	CAGTG	9 cut(s)	154, 531, 901, 1266, 2035, 2187, 2256, 2382, 2489
BveI	ACCTGC	1 cut(s)	1503
Cac8I	GCNNGC	4 cut(s)	391, 828, 928, 942
CaiI	CAGNNNCTG	3 cut(s)	530, 1433, 2186
CfoI	GCGC	2 cut(s)	280, 2198
Cfr13I	GGNCC	1 cut(s)	2503
ClaI	ATCGAT	1 cut(s)	1982
CseI	GACGC	1 cut(s)	305
Csp6I	GTAC	4 cut(s)	1625, 2347, 2614, 2681
CspCI	CAANNNNNGTGG	4 cut(s)	1110, 1145, 2447, 2482
CviQI	GTAC	4 cut(s)	1625, 2347, 2614, 2681
DdeI	CTNAG	5 cut(s)	96, 831, 1373, 2013, 2278
DraIII	CACNNNGTG	1 cut(s)	2495
DrdI	GACNNNNNNGTC	1 cut(s)	1872
DriI	GACNNNNNGTC	1 cut(s)	571
DseDI	GACNNNNNNGTC	1 cut(s)	1872
EaeI	YGGCCR	5 cut(s)	1089, 1308, 1892, 2913, 2924
Eam1104I	CTCTTC	2 cut(s)	1184, 2070
Eam1105I	GACNNNNNGTC	1 cut(s)	571
EarI	CTCTTC	2 cut(s)	1184, 2070
Eco130I	CCWWGG	2 cut(s)	934, 1026
Eco24I	GRGCYC	1 cut(s)	1250
Eco31I	GGTCTC	1 cut(s)	2240
Eco57I	CTGAAG	4 cut(s)	321, 2027, 2280, 2295
Eco88I	CYCGRG	1 cut(s)	2930
EcoRI	GAATTC	1 cut(s)	2732
EcoRII	CCWGG	3 cut(s)	225, 1894, 2718
EcoT14I	CCWWGG	2 cut(s)	934, 1026
EcoT22I	ATGCAT	1 cut(s)	1772
EcoT38I	GRGCYC	1 cut(s)	1250
ErhI	CCWWGG	2 cut(s)	934, 1026
Esp3I	CGTCTC	1 cut(s)	12
FalI	AAGNNNNNCTT	6 cut(s)	353, 385, 1458, 1490, 2429, 2461
FaqI	GGGAC	2 cut(s)	1348, 2787
FauNDI	CATATG	1 cut(s)	1500
FbaI	TGATCA	5 cut(s)	217, 1584, 1950, 2289, 2802
FblI	GTMKAC	4 cut(s)	24, 1347, 2094, 2950
FokI	GGATG	7 cut(s)	917, 1114, 1718, 2107, 2260, 2309, 2342
FriOI	GRGCYC	1 cut(s)	1250
FspBI	CTAG	7 cut(s)	174, 515, 941, 971, 1416, 1655, 1755
GlaI	GCGC	2 cut(s)	279, 2197
GsaI	CCCAGC	1 cut(s)	196
GsuI	CTGGAG	2 cut(s)	209, 2992
HaeIII	GGCC	6 cut(s)	1091, 1310, 1894, 2504, 2915, 2926
HapII	CCGG	1 cut(s)	1017
HgaI	GACGC	1 cut(s)	305
HhaI	GCGC	2 cut(s)	280, 2198
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	278, 2196
HinP1I	GCGC	2 cut(s)	278, 2196
HincII	GTYRAC	3 cut(s)	25, 820, 1925
HindII	GTYRAC	3 cut(s)	25, 820, 1925
HindIII	AAGCTT	2 cut(s)	2135, 2747
HinfI	GANTC	4 cut(s)	737, 1381, 2542, 2643
HpaI	GTTAAC	1 cut(s)	820
HpaII	CCGG	1 cut(s)	1017
Hpy188I	TCNGA	9 cut(s)	43, 99, 155, 834, 1766, 2014, 2061, 2281, 2850
Hpy99I	CGWCG	2 cut(s)	701, 854
HpyAV	CCTTC	8 cut(s)	137, 196, 239, 467, 1139, 1297, 1382, 1561
HpyCH4III	ACNGT	7 cut(s)	165, 1607, 1670, 1922, 2323, 2386, 2778
HpyCH4IV	ACGT	5 cut(s)	5, 2229, 2705, 2763, 2988
HpyF10VI	GCNNNNNNNGC	7 cut(s)	872, 891, 1208, 1767, 2204, 2315, 2429
HpyF3I	CTNAG	5 cut(s)	96, 831, 1373, 2013, 2278
HpySE526I	ACGT	5 cut(s)	5, 2229, 2705, 2763, 2988
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	278, 2196
Ksp22I	TGATCA	5 cut(s)	217, 1584, 1950, 2289, 2802
KspAI	GTTAAC	1 cut(s)	820
LmnI	GCTCC	6 cut(s)	228, 1059, 1637, 1804, 2212, 2563
MaeI	CTAG	7 cut(s)	174, 515, 941, 971, 1416, 1655, 1755
MaeII	ACGT	5 cut(s)	5, 2229, 2705, 2763, 2988
MaeIII	GTNAC	8 cut(s)	293, 758, 841, 1117, 1607, 1670, 1958, 2182
MfeI	CAATTG	1 cut(s)	1122
MflI	RGATCY	3 cut(s)	1430, 1813, 1882
MhlI	GDGCHC	4 cut(s)	97, 1250, 2445, 2500
MlsI	TGGCCA	3 cut(s)	1091, 1310, 2926
MluNI	TGGCCA	3 cut(s)	1091, 1310, 2926
MmeI	TCCRAC	3 cut(s)	458, 634, 2401
Mox20I	TGGCCA	3 cut(s)	1091, 1310, 2926
Mph1103I	ATGCAT	1 cut(s)	1772
MroXI	GAANNNNTTC	2 cut(s)	791, 2765
MscI	TGGCCA	3 cut(s)	1091, 1310, 2926
MslI	CAYNNNNRTG	6 cut(s)	515, 812, 1085, 1100, 1946, 2932
Msp20I	TGGCCA	3 cut(s)	1091, 1310, 2926
MspI	CCGG	1 cut(s)	1017
MspR9I	CCNGG	3 cut(s)	227, 1896, 2720
MunI	CAATTG	1 cut(s)	1122
Mva1269I	GAATGC	1 cut(s)	2088
MvaI	CCWGG	3 cut(s)	227, 1896, 2720
MwoI	GCNNNNNNNGC	7 cut(s)	872, 891, 1208, 1767, 2204, 2315, 2429
NcoI	CCATGG	1 cut(s)	934
NdeI	CATATG	1 cut(s)	1500
NheI	GCTAGC	1 cut(s)	940
NlaIV	GGNNCC	5 cut(s)	808, 1815, 2442, 2565, 2594
NmuCI	GTSAC	6 cut(s)	293, 758, 841, 1607, 1958, 2182
NsiI	ATGCAT	1 cut(s)	1772
NspI	RCATGY	3 cut(s)	393, 830, 1931
OliI	CACNNNNGTG	2 cut(s)	812, 2932
PaeI	GCATGC	2 cut(s)	393, 830
PctI	GAATGC	1 cut(s)	2088
PdmI	GAANNNNTTC	2 cut(s)	791, 2765
PfeI	GAWTC	4 cut(s)	737, 1381, 2542, 2643
PflFI	GACNNNGTC	1 cut(s)	764
PflMI	CCANNNNNTGG	3 cut(s)	232, 1317, 2922
PfoI	TCCNGGA	1 cut(s)	225
PshAI	GACNNNNGTC	2 cut(s)	1605, 2232
PshBI	ATTAAT	1 cut(s)	2813
Psp1406I	AACGTT	1 cut(s)	2763
Psp6I	CCWGG	3 cut(s)	225, 1894, 2718
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	3 cut(s)	225, 1894, 2718
PspN4I	GGNNCC	5 cut(s)	808, 1815, 2442, 2565, 2594
PspPI	GGNCC	1 cut(s)	2503
PsrI	GAACNNNNNNTAC	2 cut(s)	1102, 1134
PstI	CTGCAG	2 cut(s)	1801, 2829
PstNI	CAGNNNCTG	3 cut(s)	530, 1433, 2186
PsuI	RGATCY	3 cut(s)	1430, 1813, 1882
PsyI	GACNNNGTC	1 cut(s)	764
RsaI	GTAC	4 cut(s)	1626, 2348, 2615, 2682
RsaNI	GTAC	4 cut(s)	1625, 2347, 2614, 2681
RseI	CAYNNNNRTG	6 cut(s)	515, 812, 1085, 1100, 1946, 2932
SalI	GTCGAC	1 cut(s)	23
Sau96I	GGNCC	1 cut(s)	2503
ScrFI	CCNGG	3 cut(s)	227, 1896, 2720
SduI	GDGCHC	4 cut(s)	97, 1250, 2445, 2500
SfcI	CTRYAG	2 cut(s)	1797, 2825
SmiMI	CAYNNNNRTG	6 cut(s)	515, 812, 1085, 1100, 1946, 2932
SmlI	CTYRAG	3 cut(s)	1727, 2604, 2750
SmoI	CTYRAG	3 cut(s)	1727, 2604, 2750
SphI	GCATGC	2 cut(s)	393, 830
SsiI	CCGC	4 cut(s)	291, 382, 1643, 2912
SspI	AATATT	2 cut(s)	103, 639
SspMI	CTAG	7 cut(s)	174, 515, 941, 971, 1416, 1655, 1755
StyD4I	CCNGG	3 cut(s)	225, 1894, 2718
StyI	CCWWGG	2 cut(s)	934, 1026
TaaI	ACNGT	7 cut(s)	165, 1607, 1670, 1922, 2323, 2386, 2778
TaiI	ACGT	5 cut(s)	8, 2232, 2708, 2766, 2991
TaqI	TCGA	5 cut(s)	24, 768, 1384, 1491, 1982
TatI	WGTACW	2 cut(s)	1624, 2346
TauI	GCSGC	1 cut(s)	2915
TfiI	GAWTC	4 cut(s)	737, 1381, 2542, 2643
TscAI	CASTG	9 cut(s)	154, 531, 901, 1273, 2035, 2187, 2263, 2389, 2496
TseFI	GTSAC	6 cut(s)	293, 758, 841, 1607, 1958, 2182
Tsp45I	GTSAC	6 cut(s)	293, 758, 841, 1607, 1958, 2182
TspGWI	ACGGA	4 cut(s)	838, 2006, 2093, 2576
TspRI	CASTG	9 cut(s)	154, 531, 901, 1273, 2035, 2187, 2263, 2389, 2496
Tth111I	GACNNNGTC	1 cut(s)	764
Van91I	CCANNNNNTGG	3 cut(s)	232, 1317, 2922
VspI	ATTAAT	1 cut(s)	2813
XapI	RAATTY	2 cut(s)	1442, 2732
XceI	RCATGY	3 cut(s)	393, 830, 1931
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1099, 1682
XmiI	GTMKAC	4 cut(s)	24, 1347, 2094, 2950
XmnI	GAANNNNTTC	2 cut(s)	791, 2765
XspI	CTAG	7 cut(s)	174, 515, 941, 971, 1416, 1655, 1755
ZraI	GACGTC	1 cut(s)	6
Zsp2I	ATGCAT	1 cut(s)	1772

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.