Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000174

Physical Location & Seq

Genomic Coordinates Reverse (-)

3139367 .. 3144370

Gene Structure 5004 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G001670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3186 bp

ATGGATTGTGGATTCTACGTGCTTGATTCGTCATGGAAATTACGATGGCCATTAGTCTTAGTTACAAGTCTCTCTCTTTGCAATAGATGCGAACCCACAGATAAGTTGTGCAAGAGTTCCAGTGTGGAAGTCAACTTGATCCTAGATGTTCGAAACAAAAGTCTTAACGTGCAGAAGAGAAAGTGGCATTCTGCCTTTGTGGCCATCTACTGCTCTAGAGCCTTCCTCAAACCTCCTGTGCTAAAAGACATCAACATCAACACCAAATTACTATCTCGCTCTCCCTCTCACACTACCATTAGTGTTGATGATTCGGTTTCGGTGCCAGACGCTAATGGCTTCAGAATAGGCCAGACAAGACTCACTGAGCTTGTGGAGGCAAAAGATTTAAACCAGCTCCATGAGTTGGGAGGGGCTCAAGGTGTAGCAGAAGCACTCGAAACTGATGTAGAGCATGGAATTCATGGCGAAGATGCTGAAGACATTCTCAAGAGAATTGAAGCGTTTGGTTCCAACACATACCAGAGACCACCAAAGAAAGGCTTCTTCCATTTTGTTTGGGAAGCCTTCAAAGACCTCACAATCTTAATCCTCTTGGGATGTGCTGCGCTTTCTCTTGGTTTTGGGATCAAAGAGCATGGTTTAAAAGAAGGTTGGATTGACGGTGGAAGCATATGTCTTGCAATAATTCTAGTTATTTCTGTTTCGGCCATAAGCAACTATAGACAGAGCATACAGTTTGACAAATTGTCAAAAGTCAGTAATAATCTCCAGATTGAGGCTGTGAGAAAGGGAAGGCGCCAACCGGTTTCGATATTTGATATTGTGGTTGGTGATGTCATTTGTTTGAAGATTGGAGATCAAGTTCCAGCTGATGGATTGCTTCAGGATGGCCATTCTTTATCAGTAGATGAATCAAGTATGACAGGGGAGAGTGACCATGTTGAAATTAGCATTACTCAGAATCCATTCTTATTTTCTGGAACTAAAATTGCTGATGGTTATGGTCGGATGCTTGTCACATCTGTTGGGATGAACACAAATTGGGGTGAAATGATGAGCAAAATCAGCCAGGACACGAGTGAAAAGACGCCTCTACAAGCACGTCTAGACAAGCTAACTTCATCTATAGGTAAAGTCGGTTTGGCAGTTGCTTTCTTAGTTCTTTCAGTCATGTTAGTTCGATACTTCACAGGGAATACCGTGGATGAGAATGGAAGCAAAATGTTCAATGGCAGCAAGACAAAAGTAGATGACATAATAAATGCTATCATTGGAATTGTAGCAGCTGCGGTTACAATTGTTGTAGTAGCAATTCCAGAAGGTCTGCCATTGGCTGTGACTCTCACTCTTGCTTATTCCATGAAGAGAATGATGGCTGATAATGCAATGGTGCGGAAGCTCTCTGCTTGTGAGACCATGGGGTCCGCTACAACAATTTGTACAGACAAAACAGGTACTCTGACCATGAACCAGATGAAGGTGACTAAGTTTTGGTTGGGAAAAGAATCTGTGGAAAAAGAAGCTTATTCATCTATTTCTCCTTGTGTTCTCGACTTTATTCAAGAAGGGGTTGCTCTCAATACAACTGGTAGTGTGTACAGGCCTAGCTTGGATTCAAAAATTGAGATCTCTGGCAGTCCAACCGAAAAGGCTATTCTATCATGGGCGATTCATGATACAAAGATGGATATGGAGAATGTGGTGAAGAGTTGTAGCATTCTCCATGTTGAGGCTTTCAATTCACAGAAGAAACGTAGTGGGGTTTTAATTAAGAGGAAGGCAGATAATACAGTCCAAGTACATTATAAAGGAGCTGCAGAGATGATACTAGCAATGTGTACAAGTTACTATGATGCCTCTGGAGTTGTTAAGGATATGGATTACAATGAAAAAATGAACTTTGAGAAGATTATTCAAGGTATGGCAGCCAGCAGCCTCAGATGCATCGCATTCGCACATAAAGAAGTTGCAGCAGAGGAGCAAGTTGAAGGAGACCAGAAAGTTGTGCTGAGGGAAGATGGTTTGACCCTATTGGGACTTGTAGGTCTTAAAGATCCATGTCGTCCAGGAGTGAAGAAAGCAGTTGAAGACTGTCAATATGCAGGTGTGAACATCAAAATGATCACTGGCGATAATGTTTTCACTGCAAAAGCCATAGCTACTGAGTGTGGGATACTCAGGCCTGGTCAGGACATGCTCGGAGCAGTCATAGAAGGTGTCGAATTTCGCAACTACACTCCAGAACAGAGAATGGAGAAGGTTGAGGCAATATGTGTAATGGCGAGGTCTTCTCCTTTTGATAAGCTTCTGATGGTACAATGCTTGAAGCAGAAAGGTCATGTAGTTGCAGTGACAGGTGATGGAACCAATGATGCACCAGCATTGAAAGAAGCTGATATAGGACTTTCTATGGGGATTCAAGGCACAGAAGTTGCTAAAGAGAGCTCGGATATTGTCATCATGGATGATAACTTTGCTTCAGTTGCTACAGTTTTGATGTGGGGAAGATGTGTGTACAATAATATCCAGAAGTTCATCCAATTCCAGCTCACTGTCAATGTTGCAGCTCTTGTTATCAACTTTGTAGCTGCAGTTTCAGCAGGTCAAGTCCCATTAACAGCAGTTCAGTTATTGTGGGTGAACTTGATCATGGACACACTTGGAGCTCTTGCTCTTGCCACAGAAAAGCCCACAAAAGAACTCATGGAGAAGCCACCAGTGGGAAGGACAGCGCCTCTCATCACAAACATCATGTGGAGGAACCTCTTGCCTCAAGCATTGTACCAGATAACAGTCCTCTTGATCCTGCAATTCAGGGGCAAAGAAATCTTTGGTGTCACTGATAAGGTAAAGGACACATTGATCTTCAACACTTTTGTGCTTTGCCAGGTTTTCAATGAGTTCAATGCGAGGAAGCTAGAGAAGAAGAATGTCTTCAAGGGAATACACACCAACAAGTTATTTATGGGAATCATTGCAGCAACAATTATTCTCCAAGTGGTGATGGTGGAGTTTCTTAAAAAATTTGCAGATACAGAGAGATTGAACTGGGGACAATGGGGTATATGCATTGGCATTGCAGTCATCTCTTGGCCAATTGGTTGGGTGTTCAAGTCAATACCTGTTCCAGAGAAATCCTTTCTCATTTATCTAAAGATGAAGAATTATAAGAGCAACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1061

Amino Acids

116.6

Weight (kDa)

7.44

Isoelectric Point (pI)

30.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	136 - 205	5.1e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	261 - 355	8.2e-19	P-type ATPase actuator domain
Hydrolase	PF00702	479 - 800	2.8e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	519 - 690	3.2e-28	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	871 - 1040	1.5e-47	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22910
fragaria_vesca	FvH4_4g01640 FvH4_4g01670 FvH4_4g01690 FvH4_4g01690 FvH4_4g01692 FvH4_4g06940
malus_domestica	MD04G1223800.v1.1 MD12G1240600.v1.1 MD13G1201600.v1.1 MD15G1425600.v1.1 MD15G1425900.v1.1 MD15G1426000.v1.1 MD15G1426200.v1.1 MD15G1429900.v1.1 MD16G1186200.v1.1 MD16G1201500.v1.1 MD16G1201800.v1.1 MD16G1245900.v1.1
prunus_persica	Prupe.1G023100_v2.0.a1 Prupe.1G023200_v2.0.a1 Prupe.1G023300_v2.0.a1 Prupe.1G023400_v2.0.a1 Prupe.2G086400_v2.0.a1
pyrus_communis	pycom03g20400 pycom04g03220 pycom04g03230 pycom04g19720 pycom13g17500 pycom14g13160 pycom15g37720
rosa_chinensis	RchiOBHm_Chr4g0385871 RchiOBHm_Chr4g0388251 RchiOBHm_Chr4g0388261 RchiOBHm_Chr4g0388291 RchiOBHm_Chr4g0388301 RchiOBHm_Chr4g0388321 RchiOBHm_Chr4g0388331 RchiOBHm_Chr4g0388341 RchiOBHm_Chr4g0388491 RchiOBHm_Chr4g0388501 RchiOBHm_Chr4g0388521 RchiOBHm_Chr4g0388541 RchiOBHm_Chr4g0388581 RchiOBHm_Chr4g0388591 RchiOBHm_Chr5g0038901 RchiOBHm_Chr5g0039001 RchiOBHm_Chr6g0264011
rosa_laevigata	RLG00000003378 RLG00000003379 RLG00000010036 RLG00000010037 RLG00000010039 RLG00000010041 RLG00000010042 RLG00000010043 RLG00000010049 RLG00000010050 RLG00000010051 RLG00000010052 RLG00000010054 RLG00000010055 RLG00000010062 RLG00000010064 RLG00000010065 RLG00000010066 RLG00000010067 RLG00000010069 RLG00000010070 RLG00000010071 RLG00000014295 RLG00000033880
rosa_multiflora	Rmu_co8110336.1_g000001 Rmu_sc0000533.1_g000070 Rmu_sc0000533.1_g000082 Rmu_sc0000753.1_g000013 Rmu_sc0000753.1_g000024 Rmu_sc0000753.1_g000027 Rmu_sc0000753.1_g000029 Rmu_sc0000753.1_g000030 Rmu_sc0000753.1_g000037 Rmu_sc0000753.1_g000048 Rmu_sc0001241.1_g000003 Rmu_sc0001241.1_g000020 Rmu_sc0001241.1_g000034 Rmu_sc0001241.1_g000045 Rmu_sc0001949.1_g000015 Rmu_sc0001949.1_g000020 Rmu_sc0001949.1_g000023 Rmu_sc0001949.1_g000028 Rmu_sc0002357.1_g000005 Rmu_sc0002357.1_g000017 Rmu_sc0002357.1_g000032 Rmu_sc0002357.1_g000035 Rmu_sc0002357.1_g000043 Rmu_sc0002694.1_g000002 Rmu_sc0004459.1_g000018 Rmu_sc0004773.1_g000011 Rmu_sc0006339.1_g000009 Rmu_sc0008728.1_g000008 Rmu_sc0012275.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042120 Rroxscaffold_2G00113240 Rroxscaffold_2G00131810 Rroxscaffold_4G00314870 Rroxscaffold_5G00334730 Rroxscaffold_5G00334750 Rroxscaffold_5G00334770 Rroxscaffold_5G00334790 Rroxscaffold_5G00334850 Rroxscaffold_5G00334970 Rroxscaffold_5G00335010 Rroxscaffold_5G00335030 Rroxscaffold_5G00335040
rosa_rugosa	Rorug03G0301800 Rorug03G0316600 Rorug03G0316600 Rorug03G0316700 Rorug03G0316700 Rorug03G0316800.1 Rorug03G0317300 Rorug03G0317400 Rorug03G0318000 Rorug03G0318500 Rorug03G0318600 Rorug03G0318700 Rorug03G0319000 Rorug03G0319000 Rorug03G0319100 Rorug05G0175000 Rorug05G0175500
rosa_samantha	Rh4CG007500 Rh4CG022300 Rh4CG022400 Rh4CG022600 Rh4CG022700 Rh4CG023500 Rh4CG023700 Rh4CG023900 Rh4CG024000 Rh4CG024600 Rh4CG025200 Rh4CG025300 Rh4CG025700 Rh4CG025800 Rh4CG025900 Rh4CG026000 Rh4CG026100 Rh4CG026200 Rh5BG266500 Rh6AG128500 Rh6DG110800
rosa_wichuraiana	Rw0G009370 Rw4G000360 Rw4G001430 Rw4G001440 Rw4G001460 Rw4G001470 Rw4G001490 Rw4G001500 Rw4G001520 Rw4G001620 Rw4G001630 Rw4G001650 Rw4G001670 Rw5G024450 Rw5G024490 Rw6G011090 Rw6G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1809, 3171
AarI	CACCTGC	1 cut(s)	2096
AasI	GACNNNNNNGTC	2 cut(s)	1423, 2046
Acc36I	ACCTGC	2 cut(s)	2096, 2594
AccB1I	GGYRCC	2 cut(s)	322, 798
AccB7I	CCANNNNNTGG	2 cut(s)	406, 875
AciI	CCGC	3 cut(s)	1292, 1396, 1428
AclWI	GGATC	4 cut(s)	133, 635, 2051, 2800
AcoI	YGGCCR	5 cut(s)	47, 201, 708, 892, 3095
AcsI	RAATTY	3 cut(s)	459, 2225, 3026
AcuI	CTGAAG	4 cut(s)	325, 498, 869, 2466
AcyI	GRCGYC	2 cut(s)	799, 1091
AfaI	GTAC	8 cut(s)	1444, 1459, 1601, 1803, 1843, 2319, 2519, 2786
AfiI	CCNNNNNNNGG	7 cut(s)	406, 539, 778, 875, 1480, 1732, 2723
AgeI	ACCGGT	1 cut(s)	805
AhdI	GACNNNNNGTC	1 cut(s)	748
AjiI	CACGTC	1 cut(s)	1106
AjnI	CCWGG	4 cut(s)	1071, 2068, 2185, 2889
AleI	CACNNNNGTG	1 cut(s)	2879
AloI	GAACNNNNNNTCC	2 cut(s)	849, 881
Alw21I	GWGCWC	2 cut(s)	2450, 2671
Alw26I	GTCTC	4 cut(s)	74, 520, 1409, 1989
AlwI	GGATC	4 cut(s)	133, 635, 2051, 2800
AoxI	GGCC	8 cut(s)	47, 201, 349, 708, 892, 1604, 2183, 3095
ApoI	RAATTY	3 cut(s)	459, 2225, 3026
ArsI	GACNNNNNNTTYG	2 cut(s)	145, 177
AsiGI	ACCGGT	1 cut(s)	805
Asp700I	GAANNNNTTC	4 cut(s)	483, 542, 968, 2936
AspLEI	GCGC	3 cut(s)	610, 801, 2737
AspS9I	GGNCC	1 cut(s)	1425
AsuHPI	GGTGA	7 cut(s)	845, 1061, 1495, 1717, 2372, 2653, 3016
AsuII	TTCGAA	1 cut(s)	151
AvaII	GGWCC	1 cut(s)	1425
BalI	TGGCCA	4 cut(s)	49, 203, 894, 3097
BanI	GGYRCC	2 cut(s)	322, 798
BanII	GRGCYC	3 cut(s)	418, 2450, 2671
BauI	CACGAG	1 cut(s)	1078
BbsI	GAAGAC	4 cut(s)	486, 2097, 2283, 2929
Bbv12I	GWGCWC	2 cut(s)	2450, 2671
BbvCI	CCTCAGC	1 cut(s)	2012
BcgI	CGANNNNNNTGC	2 cut(s)	1936, 1970
BciT130I	CCWGG	4 cut(s)	1073, 2070, 2187, 2891
BciVI	GTATCC	1 cut(s)	2169
BclI	TGATCA	2 cut(s)	2124, 2649
BcoDI	GTCTC	4 cut(s)	74, 520, 1409, 1989
BfaI	CTAG	7 cut(s)	143, 216, 692, 1109, 1608, 1832, 2921
BfmI	CTRYAG	5 cut(s)	721, 1128, 1818, 2490, 2592
BfoI	RGCGCY	2 cut(s)	802, 2738
BfuAI	ACCTGC	2 cut(s)	2096, 2594
BfuI	GTATCC	1 cut(s)	2169
BglI	GCCNNNNNGGC	1 cut(s)	200
BglII	AGATCT	1 cut(s)	1629
Bme1390I	CCNGG	4 cut(s)	1073, 2070, 2187, 2891
Bme18I	GGWCC	1 cut(s)	1425
BmeRI	GACNNNNNGTC	1 cut(s)	748
BmgBI	CACGTC	1 cut(s)	1106
BmgT120I	GGNCC	1 cut(s)	1425
BmiI	GGNNCC	6 cut(s)	324, 511, 800, 1426, 2368, 2765
BmrFI	CCNGG	4 cut(s)	1073, 2070, 2187, 2891
BmrI	ACTGGG	1 cut(s)	3061
BmsI	GCATC	7 cut(s)	77, 463, 1002, 1846, 1934, 1956, 2365
BmuI	ACTGGG	1 cut(s)	3061
BpiI	GAAGAC	4 cut(s)	486, 2097, 2283, 2929
BplI	GAGNNNNNCTC	4 cut(s)	210, 242, 2278, 2310
BpmI	CTGGAG	3 cut(s)	755, 1884, 2226
Bpu10I	CCTNAGC	1 cut(s)	2012
Bpu14I	TTCGAA	1 cut(s)	151
BpuEI	CTTGAG	3 cut(s)	402, 473, 2760
BsaAI	YACGTR	1 cut(s)	19
BsaHI	GRCGYC	2 cut(s)	799, 1091
BsaI	GGTCTC	3 cut(s)	520, 1409, 1989
BsaJI	CCNNGG	2 cut(s)	1203, 1419
BsaWI	WCCGGW	1 cut(s)	805
BsaXI	ACNNNNNCTCC	2 cut(s)	849, 879
Bsc4I	CCNNNNNNNGG	7 cut(s)	406, 539, 778, 875, 1480, 1732, 2723
Bse118I	RCCGGY	1 cut(s)	805
Bse1I	ACTGG	5 cut(s)	120, 1594, 2134, 2720, 3056
Bse3DI	GCAATG	4 cut(s)	1395, 1842, 2976, 3078
BseBI	CCWGG	4 cut(s)	1073, 2070, 2187, 2891
BseDI	CCNNGG	2 cut(s)	1203, 1419
BseGI	GGATG	7 cut(s)	605, 895, 1017, 1038, 1213, 2473, 2538
BseLI	CCNNNNNNNGG	7 cut(s)	406, 539, 778, 875, 1480, 1732, 2723
BseMI	GCAATG	4 cut(s)	1395, 1842, 2976, 3078
BseMII	CTCAG	6 cut(s)	357, 974, 1954, 2003, 2157, 2194
BseNI	ACTGG	5 cut(s)	120, 1594, 2134, 2720, 3056
BseRI	GAGGAG	1 cut(s)	1994
BsgI	GTGCAG	1 cut(s)	191
BshFI	GGCC	8 cut(s)	49, 203, 351, 710, 894, 1606, 2185, 3097
BshNI	GGYRCC	2 cut(s)	322, 798
BshTI	ACCGGT	1 cut(s)	805
BsiHKAI	GWGCWC	2 cut(s)	2450, 2671
BsiSI	CCGG	1 cut(s)	806
BslFI	GGGAC	3 cut(s)	2052, 2597, 3069
BslI	CCNNNNNNNGG	7 cut(s)	406, 539, 778, 875, 1480, 1732, 2723
BsmAI	GTCTC	4 cut(s)	74, 520, 1409, 1989
BsmFI	GGGAC	3 cut(s)	2052, 2597, 3069
BsmI	GAATGC	3 cut(s)	187, 1719, 1952
BsnI	GGCC	8 cut(s)	49, 203, 351, 710, 894, 1606, 2185, 3097
Bso31I	GGTCTC	3 cut(s)	520, 1409, 1989
Bsp119I	TTCGAA	1 cut(s)	151
Bsp1286I	GDGCHC	3 cut(s)	418, 2450, 2671
Bsp1407I	TGTACA	4 cut(s)	1442, 1599, 1841, 2517
Bsp143I	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
Bsp19I	CCATGG	1 cut(s)	1419
BspACI	CCGC	3 cut(s)	1292, 1396, 1428
BspANI	GGCC	8 cut(s)	49, 203, 351, 710, 894, 1606, 2185, 3097
BspCNI	CTCAG	6 cut(s)	358, 973, 1953, 2004, 2158, 2193
BspHI	TCATGA	1 cut(s)	1675
BspLI	GGNNCC	6 cut(s)	324, 511, 800, 1426, 2368, 2765
BspMAI	CTGCAG	2 cut(s)	1822, 2596
BspMI	ACCTGC	2 cut(s)	2096, 2594
BspPI	GGATC	4 cut(s)	133, 635, 2051, 2800
BspT104I	TTCGAA	1 cut(s)	151
BspT107I	GGYRCC	2 cut(s)	322, 798
BspTNI	GGTCTC	3 cut(s)	520, 1409, 1989
BsrDI	GCAATG	4 cut(s)	1395, 1842, 2976, 3078
BsrFI	RCCGGY	1 cut(s)	805
BsrGI	TGTACA	4 cut(s)	1442, 1599, 1841, 2517
BsrI	ACTGG	5 cut(s)	120, 1594, 2134, 2720, 3056
BssAI	RCCGGY	1 cut(s)	805
BssECI	CCNNGG	2 cut(s)	1203, 1419
BssMI	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
BssNI	GRCGYC	2 cut(s)	799, 1091
BssSI	CACGAG	1 cut(s)	1078
BssT1I	CCWWGG	1 cut(s)	1419
Bst2BI	CACGAG	1 cut(s)	1078
Bst2UI	CCWGG	4 cut(s)	1073, 2070, 2187, 2891
Bst4CI	ACNGT	8 cut(s)	665, 738, 1204, 1795, 2096, 2494, 2557, 2797
Bst6I	CTCTTC	3 cut(s)	170, 1361, 1703
BstACI	GRCGYC	2 cut(s)	799, 1091
BstAPI	GCANNNNNTGC	1 cut(s)	87
BstAUI	TGTACA	4 cut(s)	1442, 1599, 1841, 2517
BstBAI	YACGTR	1 cut(s)	19
BstBI	TTCGAA	1 cut(s)	151
BstC8I	GCNNGC	1 cut(s)	1933
BstDEI	CTNAG	9 cut(s)	58, 366, 960, 1159, 1488, 1940, 2012, 2166, 2180
BstDSI	CCRYGG	2 cut(s)	1203, 1419
BstF5I	GGATG	7 cut(s)	605, 895, 1017, 1038, 1213, 2473, 2538
BstH2I	RGCGCY	2 cut(s)	802, 2738
BstHHI	GCGC	3 cut(s)	610, 801, 2737
BstKTI	GATC	9 cut(s)	141, 630, 862, 1632, 2059, 2127, 2652, 2808, 2868
BstMAI	GTCTC	4 cut(s)	74, 520, 1409, 1989
BstMBI	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
BstMWI	GCNNNNNNNGC	7 cut(s)	87, 200, 1068, 1385, 1944, 2486, 2600
BstNI	CCWGG	4 cut(s)	1073, 2070, 2187, 2891
BstNSI	RCATGY	1 cut(s)	2200
BstSCI	CCNGG	4 cut(s)	1071, 2068, 2185, 2889
BstSFI	CTRYAG	5 cut(s)	721, 1128, 1818, 2490, 2592
BstV2I	GAAGAC	4 cut(s)	486, 2097, 2283, 2929
BstX2I	RGATCY	2 cut(s)	1629, 2056
BstXI	CCANNNNNNTGG	1 cut(s)	3105
BstYI	RGATCY	2 cut(s)	1629, 2056
BsuI	GTATCC	1 cut(s)	2169
BsuRI	GGCC	8 cut(s)	49, 203, 351, 710, 894, 1606, 2185, 3097
BtgI	CCRYGG	2 cut(s)	1203, 1419
BtgZI	GCGATG	1 cut(s)	1933
BtrI	CACGTC	1 cut(s)	1106
BtsCI	GGATG	7 cut(s)	605, 895, 1017, 1038, 1213, 2473, 2538
BtsI	GCAGTG	2 cut(s)	2145, 2358
BtsIMutI	CAGTG	8 cut(s)	127, 363, 2127, 2145, 2358, 2553, 2727, 2841
BveI	ACCTGC	2 cut(s)	2096, 2594
Cac8I	GCNNGC	1 cut(s)	1933
CciI	TCATGA	1 cut(s)	1675
CfoI	GCGC	3 cut(s)	610, 801, 2737
Cfr10I	RCCGGY	1 cut(s)	805
Cfr13I	GGNCC	1 cut(s)	1425
CseI	GACGC	2 cut(s)	338, 1099
Csp6I	GTAC	8 cut(s)	1443, 1458, 1600, 1802, 1842, 2318, 2518, 2785
CspAI	ACCGGT	1 cut(s)	805
CviQI	GTAC	8 cut(s)	1443, 1458, 1600, 1802, 1842, 2318, 2518, 2785
DdeI	CTNAG	9 cut(s)	58, 366, 960, 1159, 1488, 1940, 2012, 2166, 2180
DinI	GGCGCC	1 cut(s)	800
DpnI	GATC	9 cut(s)	140, 629, 861, 1631, 2058, 2126, 2651, 2807, 2867
DpnII	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
DraI	TTTAAA	2 cut(s)	390, 645
DrdI	GACNNNNNNGTC	2 cut(s)	1423, 2046
DriI	GACNNNNNGTC	1 cut(s)	748
DseDI	GACNNNNNNGTC	2 cut(s)	1423, 2046
EaeI	YGGCCR	5 cut(s)	47, 201, 708, 892, 3095
Eam1104I	CTCTTC	3 cut(s)	170, 1361, 1703
Eam1105I	GACNNNNNGTC	1 cut(s)	748
EarI	CTCTTC	3 cut(s)	170, 1361, 1703
Ecl136II	GAGCTC	2 cut(s)	2448, 2669
Eco130I	CCWWGG	1 cut(s)	1419
Eco147I	AGGCCT	2 cut(s)	1606, 2185
Eco24I	GRGCYC	3 cut(s)	418, 2450, 2671
Eco31I	GGTCTC	3 cut(s)	520, 1409, 1989
Eco47I	GGWCC	1 cut(s)	1425
Eco53kI	GAGCTC	2 cut(s)	2448, 2669
Eco57I	CTGAAG	4 cut(s)	325, 498, 869, 2466
EcoICRI	GAGCTC	2 cut(s)	2448, 2669
EcoRI	GAATTC	1 cut(s)	459
EcoRII	CCWGG	4 cut(s)	1071, 2068, 2185, 2889
EcoT14I	CCWWGG	1 cut(s)	1419
EcoT22I	ATGCAT	2 cut(s)	1949, 3074
EcoT38I	GRGCYC	3 cut(s)	418, 2450, 2671
EgeI	GGCGCC	1 cut(s)	800
EheI	GGCGCC	1 cut(s)	800
ErhI	CCWWGG	1 cut(s)	1419
FalI	AAGNNNNNCTT	4 cut(s)	527, 559, 2921, 2953
FaqI	GGGAC	3 cut(s)	2052, 2597, 3069
FauNDI	CATATG	1 cut(s)	674
FbaI	TGATCA	2 cut(s)	2124, 2649
FokI	GGATG	7 cut(s)	612, 902, 1024, 1045, 1220, 2480, 2525
FriOI	GRGCYC	3 cut(s)	418, 2450, 2671
FspBI	CTAG	7 cut(s)	143, 216, 692, 1109, 1608, 1832, 2921
GlaI	GCGC	3 cut(s)	609, 800, 2736
GsuI	CTGGAG	3 cut(s)	755, 1884, 2226
HaeII	RGCGCY	2 cut(s)	802, 2738
HaeIII	GGCC	8 cut(s)	49, 203, 351, 710, 894, 1606, 2185, 3097
HapII	CCGG	1 cut(s)	806
HgaI	GACGC	2 cut(s)	338, 1099
HhaI	GCGC	3 cut(s)	610, 801, 2737
Hin1I	GRCGYC	2 cut(s)	799, 1091
Hin6I	GCGC	3 cut(s)	608, 799, 2735
HinP1I	GCGC	3 cut(s)	608, 799, 2735
HincII	GTYRAC	1 cut(s)	133
HindII	GTYRAC	1 cut(s)	133
HindIII	AAGCTT	2 cut(s)	1524, 2306
HpaII	CCGG	1 cut(s)	806
HphI	GGTGA	7 cut(s)	845, 1061, 1495, 1717, 2372, 2653, 3016
Hpy166II	GTNNAC	6 cut(s)	133, 1600, 1842, 2113, 2518, 2644
Hpy188I	TCNGA	8 cut(s)	344, 963, 1011, 1464, 1943, 2204, 2313, 2452
Hpy8I	GTNNAC	6 cut(s)	133, 1600, 1842, 2113, 2518, 2644
HpyCH4III	ACNGT	8 cut(s)	665, 738, 1204, 1795, 2096, 2494, 2557, 2797
HpyCH4IV	ACGT	4 cut(s)	18, 168, 1105, 1756
HpyF10VI	GCNNNNNNNGC	7 cut(s)	87, 200, 1068, 1385, 1944, 2486, 2600
HpyF3I	CTNAG	9 cut(s)	58, 366, 960, 1159, 1488, 1940, 2012, 2166, 2180
HpySE526I	ACGT	4 cut(s)	18, 168, 1105, 1756
Hsp92I	GRCGYC	2 cut(s)	799, 1091
HspAI	GCGC	3 cut(s)	608, 799, 2735
KasI	GGCGCC	1 cut(s)	798
Ksp22I	TGATCA	2 cut(s)	2124, 2649
Kzo9I	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
LmnI	GCTCC	5 cut(s)	402, 1814, 1981, 2204, 2666
LweI	GCATC	7 cut(s)	77, 463, 1002, 1846, 1934, 1956, 2365
MaeI	CTAG	7 cut(s)	143, 216, 692, 1109, 1608, 1832, 2921
MaeII	ACGT	4 cut(s)	18, 168, 1105, 1756
MaeIII	GTNAC	9 cut(s)	61, 935, 1018, 1294, 1339, 1483, 1847, 2353, 2839
MalI	GATC	9 cut(s)	140, 629, 861, 1631, 2058, 2126, 2651, 2807, 2867
MboI	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
MfeI	CAATTG	2 cut(s)	1299, 3099
MflI	RGATCY	2 cut(s)	1629, 2056
MhlI	GDGCHC	3 cut(s)	418, 2450, 2671
MlsI	TGGCCA	4 cut(s)	49, 203, 894, 3097
MluNI	TGGCCA	4 cut(s)	49, 203, 894, 3097
Mly113I	GGCGCC	1 cut(s)	799
MlyI	GAGTC	2 cut(s)	354, 1336
MmeI	TCCRAC	4 cut(s)	537, 635, 989, 1667
Mox20I	TGGCCA	4 cut(s)	49, 203, 894, 3097
Mph1103I	ATGCAT	2 cut(s)	1949, 3074
MroXI	GAANNNNTTC	4 cut(s)	483, 542, 968, 2936
MscI	TGGCCA	4 cut(s)	49, 203, 894, 3097
MslI	CAYNNNNRTG	2 cut(s)	2120, 2879
Msp20I	TGGCCA	4 cut(s)	49, 203, 894, 3097
MspA1I	CMGCKG	2 cut(s)	872, 1289
MspI	CCGG	1 cut(s)	806
MspR9I	CCNGG	4 cut(s)	1073, 2070, 2187, 2891
MunI	CAATTG	2 cut(s)	1299, 3099
Mva1269I	GAATGC	3 cut(s)	187, 1719, 1952
MvaI	CCWGG	4 cut(s)	1073, 2070, 2187, 2891
MwoI	GCNNNNNNNGC	7 cut(s)	87, 200, 1068, 1385, 1944, 2486, 2600
NarI	GGCGCC	1 cut(s)	799
NcoI	CCATGG	1 cut(s)	1419
NdeI	CATATG	1 cut(s)	674
NdeII	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
NlaIV	GGNNCC	6 cut(s)	324, 511, 800, 1426, 2368, 2765
NmuCI	GTSAC	6 cut(s)	935, 1018, 1339, 1483, 2353, 2839
NsiI	ATGCAT	2 cut(s)	1949, 3074
NspI	RCATGY	1 cut(s)	2200
NspV	TTCGAA	1 cut(s)	151
OliI	CACNNNNGTG	1 cut(s)	2879
PacI	TTAATTAA	1 cut(s)	1773
PagI	TCATGA	1 cut(s)	1675
PaqCI	CACCTGC	1 cut(s)	2096
PceI	AGGCCT	2 cut(s)	1606, 2185
PctI	GAATGC	3 cut(s)	187, 1719, 1952
PdmI	GAANNNNTTC	4 cut(s)	483, 542, 968, 2936
PflMI	CCANNNNNTGG	2 cut(s)	406, 875
PfoI	TCCNGGA	1 cut(s)	2068
PinAI	ACCGGT	1 cut(s)	805
PleI	GAGTC	2 cut(s)	354, 1336
PluTI	GGCGCC	1 cut(s)	802
PpsI	GAGTC	2 cut(s)	354, 1336
Ppu21I	YACGTR	1 cut(s)	19
PsiI	TTATAA	2 cut(s)	1809, 3171
Psp124BI	GAGCTC	2 cut(s)	2450, 2671
Psp6I	CCWGG	4 cut(s)	1071, 2068, 2185, 2889
PspGI	CCWGG	4 cut(s)	1071, 2068, 2185, 2889
PspN4I	GGNNCC	6 cut(s)	324, 511, 800, 1426, 2368, 2765
PspPI	GGNCC	1 cut(s)	1425
PstI	CTGCAG	2 cut(s)	1822, 2596
PsuI	RGATCY	2 cut(s)	1629, 2056
PvuII	CAGCTG	2 cut(s)	872, 1289
RsaI	GTAC	8 cut(s)	1444, 1459, 1601, 1803, 1843, 2319, 2519, 2786
RsaNI	GTAC	8 cut(s)	1443, 1458, 1600, 1802, 1842, 2318, 2518, 2785
RseI	CAYNNNNRTG	2 cut(s)	2120, 2879
SacI	GAGCTC	2 cut(s)	2450, 2671
Sau3AI	GATC	9 cut(s)	138, 627, 859, 1629, 2056, 2124, 2649, 2805, 2865
Sau96I	GGNCC	1 cut(s)	1425
SchI	GAGTC	2 cut(s)	354, 1336
ScrFI	CCNGG	4 cut(s)	1073, 2070, 2187, 2891
SduI	GDGCHC	3 cut(s)	418, 2450, 2671
SfaNI	GCATC	7 cut(s)	77, 463, 1002, 1846, 1934, 1956, 2365
SfcI	CTRYAG	5 cut(s)	721, 1128, 1818, 2490, 2592
SfoI	GGCGCC	1 cut(s)	800
SfuI	TTCGAA	1 cut(s)	151
SinI	GGWCC	1 cut(s)	1425
SmiMI	CAYNNNNRTG	2 cut(s)	2120, 2879
SmlI	CTYRAG	3 cut(s)	417, 488, 2775
SmoI	CTYRAG	3 cut(s)	417, 488, 2775
SseBI	AGGCCT	2 cut(s)	1606, 2185
SsiI	CCGC	3 cut(s)	1292, 1396, 1428
SspDI	GGCGCC	1 cut(s)	798
SspMI	CTAG	7 cut(s)	143, 216, 692, 1109, 1608, 1832, 2921
SstI	GAGCTC	2 cut(s)	2450, 2671
StuI	AGGCCT	2 cut(s)	1606, 2185
StyD4I	CCNGG	4 cut(s)	1071, 2068, 2185, 2889
StyI	CCWWGG	1 cut(s)	1419
TaaI	ACNGT	8 cut(s)	665, 738, 1204, 1795, 2096, 2494, 2557, 2797
TaiI	ACGT	4 cut(s)	21, 171, 1108, 1759
TaqI	TCGA	6 cut(s)	151, 438, 812, 1183, 1554, 2223
TatI	WGTACW	5 cut(s)	1442, 1599, 1801, 1841, 2517
TscAI	CASTG	8 cut(s)	127, 370, 2134, 2152, 2358, 2560, 2727, 2848
TseFI	GTSAC	6 cut(s)	935, 1018, 1339, 1483, 2353, 2839
Tsp45I	GTSAC	6 cut(s)	935, 1018, 1339, 1483, 2353, 2839
TspRI	CASTG	8 cut(s)	127, 370, 2134, 2152, 2358, 2560, 2727, 2848
Van91I	CCANNNNNTGG	2 cut(s)	406, 875
VpaK11BI	GGWCC	1 cut(s)	1425
XapI	RAATTY	3 cut(s)	459, 2225, 3026
XbaI	TCTAGA	2 cut(s)	215, 1108
XceI	RCATGY	1 cut(s)	2200
XmnI	GAANNNNTTC	4 cut(s)	483, 542, 968, 2936
XspI	CTAG	7 cut(s)	143, 216, 692, 1109, 1608, 1832, 2921
Zsp2I	ATGCAT	2 cut(s)	1949, 3074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.