pycom05g08340

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000250
Physical Location & Seq
Forward (+)
10956013 .. 10957680
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08340.3

Sequence Viewer

Length: 1227 bp
ATGTGTACCGACTACTTCATCAAAAGGAGCGCTTCATATGGAATAACATCATCTACTGATTTGGTATCCCATAATCTATCGTCCCCGACAATAGCCCGCAATTTATGGGCAAAAACTTGGCGAAGTTCTTCCAAAGATATGGCATCAAGCAGGACATGTCTACGCCTAGATATCCCCAAGGCAACAGAAAAATCCCTCACAGACAAGCAGGGAAATTGGCCAGACGAACTTCCTAGTGTCTTATGGGCATATCTAGCATTTGGTCAAAAGAAATCATCCCTCCCAATGCATCGTGTTAAGCATCAACACCATACTACTAACTTTAGAGCAGAACAAAAAAGAGATGGCCAAATATCTAGATCTGGCAAAGGAGAAACTCGAGAAGGTTATCACTTACAATGTAGACTACAGCAGCAGCTCATCTCTAGCTACAATAGAAGGGCAAAGACCCAGCAGTTTCAGCCTAGAGATCTAGTCCTAAGAAAAGAAAGTCCCTACAAAATCAGCAGAGCAGGCGGCAATGATAGCTACACCCTCACCACCATGAACGACAAAAAGATCGACAAGCAGTGGAATGCCTACAACCTGCAAAAGTACTATTCAAAGACTCAAGCAGCTCGACGAGCACTACCTCAAAAGCCAAGGACTACCCAATTGTTATGCAATTCTACCTTTCAACTAAGTTCGATATCCTTCATCTTATTTTCAATGAAGATTTCCGAAGCTATATATCACTTGGCTCAACTACGTATCTGCAACAATTATCTACTCCTACTCACATTGAAGATCACACCTAATCAAGGTCCTATCGCAAGAATTCTAAACGTGGATGGGTCAGGTTGCCCTGGTGTAACTAAAGGCATCACTAGAAGTAGCCACCTAACGATCTCTGAACCTAAAGTCCCTGTCTATTCAACGAGCAGCCAACAGTTTCTGGAAAGTACCTGGGAACCTAAAACCTCTACGAATTCAACGAGAAGCCTACGAATTCTAGAACATCCTTGGGAATCTAAAGTTGCTGCCAATTCACCAAGCAACCTACGGTTTCTAGAAAACTGTCTACGGTACACCCTTCGAGCAATTAGCCCAGGTTATCTCGCATATACACTGCCTAGCGCAGAACAACCCCTTGGGTTTGCACTGATTCTTAAAAAAGTGCAAAGTGTTCTTTCGAACCACTGCCCACGAATTTCCATAGAACCATGTACTTTTAATTCGAAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

409

Amino Acids

46.27

Weight (kDa)

9.93

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000250)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22373 FvH4_1g22374 FvH4_1g25831 FvH4_1g28031 FvH4_2g11354 FvH4_3g11301 FvH4_3g11302 FvH4_3g14364 FvH4_3g20862 FvH4_4g15482 FvH4_4g15483 FvH4_4g15484 FvH4_5g25502 FvH4_5g37242 FvH4_6g18432 FvH4_6g18433 FvH4_6g20952 FvH4_7g02201 FvH4_7g10991
prunus_persica Prupe.6G166700_v2.0.a1
pyrus_communis pycom01g03860 pycom02g16130 pycom05g08340 pycom07g08280 pycom16g20980 pycom16g25810
rosa_chinensis RchiOBHm_Chr5g0018261
rosa_laevigata RLG00000022447
rosa_multiflora Rmu_co7963284.1_g000001 Rmu_co7968088.1_g000001 Rmu_co8036800.1_g000001 Rmu_co8110294.1_g000001 Rmu_co8291955.1_g000001 Rmu_co8396209.1_g000002 Rmu_co8413879.1_g000001 Rmu_co8464165.1_g000001 Rmu_co8469565.1_g000001 Rmu_co8485695.1_g000003 Rmu_co8486821.1_g000003 Rmu_co8515379.1_g000001 Rmu_sc0000239.1_g000074 Rmu_sc0000327.1_g000028 Rmu_sc0000509.1_g000002 Rmu_sc0000677.1_g000042 Rmu_sc0000756.1_g000032 Rmu_sc0000782.1_g000013 Rmu_sc0001144.1_g000044 Rmu_sc0001861.1_g000003 Rmu_sc0002371.1_g000016 Rmu_sc0002451.1_g000061 Rmu_sc0002764.1_g000053 Rmu_sc0002868.1_g000041 Rmu_sc0003735.1_g000026 Rmu_sc0004654.1_g000006 Rmu_sc0005038.1_g000020 Rmu_sc0005236.1_g000001 Rmu_sc0006725.1_g000004 Rmu_sc0010685.1_g000001 Rmu_sc0011973.1_g000010 Rmu_sc0012722.1_g000001 Rmu_sc0015925.1_g000002 Rmu_sc0017188.1_g000001 Rmu_sc0021806.1_g000001 Rmu_sc0033125.1_g000001 Rmu_sc0038204.1_g000001 Rmu_ssc0000215.1_g000038
rosa_roxburghii Rroxscaffold_1G00001020 Rroxscaffold_1G00027520 Rroxscaffold_3G00228140 Rroxscaffold_4G00320850 Rroxscaffold_4G00321830 Rroxscaffold_4G00321840 Rroxscaffold_4G00332450 Rroxscaffold_5G00344360 Rroxscaffold_5G00365190 Rroxscaffold_5G00377320 Rroxscaffold_5G00377330 Rroxscaffold_7G00183080 Rroxscaffold_7G00184560 Rroxscaffold_7G00216010
rosa_rugosa Rorug02G0381000
rosa_samantha Rh1AG155000 Rh1AG167000 Rh2AG095000 Rh3BG335500 Rh4AG082900 Rh4CG090200 Rh5CG445500 Rh7DG403200
rosa_wichuraiana Rw0G001000 Rw0G014090 Rw0G019800 Rw0G020130 Rw0G020350 Rw1G002560 Rw1G013310 Rw1G014300 Rw1G014670 Rw1G016610 Rw1G021110 Rw1G024950 Rw1G028180 Rw2G020910 Rw2G024170 Rw2G024770 Rw2G029990 Rw2G031960 Rw2G034100 Rw3G013950 Rw3G028980 Rw3G029200 Rw4G004150 Rw4G008130 Rw4G012200 Rw4G018750 Rw4G019330 Rw4G021010 Rw4G022120 Rw4G030810 Rw5G015840 Rw5G015850 Rw5G022430 Rw5G022480 Rw5G026870 Rw5G033550 Rw6G009170 Rw6G010190 Rw6G011570 Rw6G018280 Rw6G023730 Rw7G025660 Rw7G029890 Rw7G031130 Rw7G042550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 594
AccI GTMKAC 3 cut(s) 160, 403, 1060
AciI CCGC 2 cut(s) 97, 516
AcoI YGGCCR 2 cut(s) 218, 346
AcsI RAATTY 4 cut(s) 816, 967, 987, 1188
AfaI GTAC 5 cut(s) 7, 596, 943, 1067, 1207
AfeI AGCGCT 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 800
AflIII ACRYGT 1 cut(s) 155
AgsI TTSAA 6 cut(s) 603, 677, 708, 784, 915, 972
AjnI CCWGG 3 cut(s) 844, 944, 1087
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
AloI GAACNNNNNNTCC 2 cut(s) 885, 917
AluBI AGCT 5 cut(s) 418, 429, 528, 617, 725
AluI AGCT 5 cut(s) 418, 429, 528, 617, 725
Alw21I GWGCWC 1 cut(s) 628
AlwNI CAGNNNCTG 1 cut(s) 934
Ama87I CYCGRG 1 cut(s) 378
Aor51HI AGCGCT 1 cut(s) 31
AoxI GGCC 2 cut(s) 218, 346
ApeKI GCWGC 5 cut(s) 412, 415, 614, 921, 1019
ApoI RAATTY 4 cut(s) 816, 967, 987, 1188
Asp700I GAANNNNTTC 1 cut(s) 127
AspLEI GCGC 2 cut(s) 32, 1118
AspS9I GGNCC 1 cut(s) 803
AsuHPI GGTGA 2 cut(s) 529, 1020
AsuII TTCGAA 2 cut(s) 1172, 1217
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 1 cut(s) 803
BalI TGGCCA 2 cut(s) 220, 348
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 5 cut(s) 424, 427, 626, 933, 1006
BccI CCATC 2 cut(s) 338, 824
BciT130I CCWGG 3 cut(s) 846, 946, 1089
BciVI GTATCC 1 cut(s) 76
BfmI CTRYAG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 594
BfuI GTATCC 1 cut(s) 76
BglII AGATCT 2 cut(s) 359, 469
BisI GCNGC 6 cut(s) 413, 416, 517, 615, 922, 1020
BlsI GCNGC 6 cut(s) 414, 417, 518, 616, 923, 1021
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 3 cut(s) 846, 946, 1089
Bme18I GGWCC 1 cut(s) 803
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 803
BmiI GGNNCC 1 cut(s) 951
BmrFI CCNGG 3 cut(s) 846, 946, 1089
BmsI GCATC 4 cut(s) 152, 298, 310, 870
Bpu14I TTCGAA 2 cut(s) 1172, 1217
BpuEI CTTGAG 1 cut(s) 594
BsaAI YACGTR 1 cut(s) 749
BsaJI CCNNGG 7 cut(s) 177, 641, 844, 945, 1001, 1087, 1129
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse3DI GCAATG 1 cut(s) 526
BseBI CCWGG 3 cut(s) 846, 946, 1089
BseDI CCNNGG 7 cut(s) 177, 641, 844, 945, 1001, 1087, 1129
BseGI GGATG 3 cut(s) 275, 835, 997
BseLI CCNNNNNNNGG 1 cut(s) 800
BseMI GCAATG 1 cut(s) 526
BseXI GCAGC 5 cut(s) 424, 427, 626, 933, 1006
BseYI CCCAGC 1 cut(s) 450
BshFI GGCC 2 cut(s) 220, 348
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 378
BslFI GGGAC 3 cut(s) 67, 477, 887
BslI CCNNNNNNNGG 1 cut(s) 800
BsmFI GGGAC 3 cut(s) 67, 477, 887
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 220, 348
BsoBI CYCGRG 1 cut(s) 378
Bsp119I TTCGAA 2 cut(s) 1172, 1217
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 5 cut(s) 359, 469, 558, 786, 885
BspACI CCGC 2 cut(s) 97, 516
BspANI GGCC 2 cut(s) 220, 348
BspLI GGNNCC 1 cut(s) 951
BspMI ACCTGC 1 cut(s) 594
BspT104I TTCGAA 2 cut(s) 1172, 1217
BsrDI GCAATG 1 cut(s) 526
BssECI CCNNGG 7 cut(s) 177, 641, 844, 945, 1001, 1087, 1129
BssMI GATC 5 cut(s) 359, 469, 558, 786, 885
BssT1I CCWWGG 4 cut(s) 177, 641, 1001, 1129
Bst2UI CCWGG 3 cut(s) 846, 946, 1089
Bst4CI ACNGT 4 cut(s) 930, 1044, 1058, 1065
BstBAI YACGTR 1 cut(s) 749
BstBI TTCGAA 2 cut(s) 1172, 1217
BstC8I GCNNGC 2 cut(s) 97, 514
BstDEI CTNAG 2 cut(s) 479, 680
BstENI CCTNNNNNAGG 1 cut(s) 798
BstF5I GGATG 3 cut(s) 275, 835, 997
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 2 cut(s) 32, 1118
BstKTI GATC 5 cut(s) 362, 472, 561, 789, 888
BstMBI GATC 5 cut(s) 359, 469, 558, 786, 885
BstMWI GCNNNNNNNGC 5 cut(s) 254, 460, 513, 525, 623
BstNI CCWGG 3 cut(s) 846, 946, 1089
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 3 cut(s) 844, 944, 1087
BstSFI CTRYAG 1 cut(s) 407
BstSNI TACGTA 1 cut(s) 749
BstV1I GCAGC 5 cut(s) 424, 427, 626, 933, 1006
BstX2I RGATCY 2 cut(s) 359, 469
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 2 cut(s) 359, 469
BsuI GTATCC 1 cut(s) 76
BsuRI GGCC 2 cut(s) 220, 348
BtsCI GGATG 3 cut(s) 275, 835, 997
BtsI GCAGTG 3 cut(s) 575, 1106, 1177
BtsIMutI CAGTG 4 cut(s) 575, 1106, 1139, 1177
BveI ACCTGC 1 cut(s) 594
Cac8I GCNNGC 2 cut(s) 97, 514
CaiI CAGNNNCTG 1 cut(s) 934
CfoI GCGC 2 cut(s) 32, 1118
Cfr13I GGNCC 1 cut(s) 803
Csp6I GTAC 5 cut(s) 6, 595, 942, 1066, 1206
CviAII CATG 3 cut(s) 156, 544, 1203
CviQI GTAC 5 cut(s) 6, 595, 942, 1066, 1206
DdeI CTNAG 2 cut(s) 479, 680
DpnI GATC 5 cut(s) 361, 471, 560, 788, 887
DpnII GATC 5 cut(s) 359, 469, 558, 786, 885
EaeI YGGCCR 2 cut(s) 218, 346
Eco105I TACGTA 1 cut(s) 749
Eco130I CCWWGG 4 cut(s) 177, 641, 1001, 1129
Eco32I GATATC 2 cut(s) 172, 690
Eco47I GGWCC 1 cut(s) 803
Eco47III AGCGCT 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 803
EcoRI GAATTC 3 cut(s) 816, 967, 987
EcoRII CCWGG 3 cut(s) 844, 944, 1087
EcoRV GATATC 2 cut(s) 172, 690
EcoT14I CCWWGG 4 cut(s) 177, 641, 1001, 1129
EcoT22I ATGCAT 1 cut(s) 291
ErhI CCWWGG 4 cut(s) 177, 641, 1001, 1129
FaeI CATG 3 cut(s) 159, 547, 1206
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FaqI GGGAC 3 cut(s) 67, 477, 887
FatI CATG 3 cut(s) 155, 543, 1202
FauI CCCGC 1 cut(s) 104
FauNDI CATATG 1 cut(s) 37
FblI GTMKAC 3 cut(s) 160, 403, 1060
Fnu4HI GCNGC 6 cut(s) 413, 416, 517, 615, 922, 1020
FokI GGATG 3 cut(s) 262, 842, 984
Fsp4HI GCNGC 6 cut(s) 413, 416, 517, 615, 922, 1020
GlaI GCGC 2 cut(s) 31, 1117
GluI GCNGC 6 cut(s) 413, 416, 517, 615, 922, 1020
GsaI CCCAGC 1 cut(s) 454
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 2 cut(s) 220, 348
HhaI GCGC 2 cut(s) 32, 1118
Hin1II CATG 3 cut(s) 159, 547, 1206
Hin6I GCGC 2 cut(s) 30, 1116
HinP1I GCGC 2 cut(s) 30, 1116
HinfI GANTC 3 cut(s) 607, 1007, 1144
HphI GGTGA 2 cut(s) 529, 1020
Hpy166II GTNNAC 5 cut(s) 6, 161, 404, 1061, 1068
Hpy188I TCNGA 2 cut(s) 721, 892
Hpy188III TCNNGA 5 cut(s) 357, 380, 935, 992, 1049
Hpy8I GTNNAC 5 cut(s) 6, 161, 404, 1061, 1068
Hpy99I CGWCG 1 cut(s) 624
HpyAV CCTTC 4 cut(s) 377, 432, 703, 1082
HpyCH4III ACNGT 4 cut(s) 930, 1044, 1058, 1065
HpyCH4IV ACGT 2 cut(s) 748, 825
HpyCH4V TGCA 6 cut(s) 289, 589, 663, 756, 1139, 1159
HpyF10VI GCNNNNNNNGC 5 cut(s) 254, 460, 513, 525, 623
HpyF3I CTNAG 2 cut(s) 479, 680
HpySE526I ACGT 2 cut(s) 748, 825
Hsp92II CATG 3 cut(s) 159, 547, 1206
HspAI GCGC 2 cut(s) 30, 1116
Kzo9I GATC 5 cut(s) 359, 469, 558, 786, 885
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 5 cut(s) 424, 427, 626, 933, 1006
LweI GCATC 4 cut(s) 152, 298, 310, 870
MaeII ACGT 2 cut(s) 748, 825
MaeIII GTNAC 1 cut(s) 850
MalI GATC 5 cut(s) 361, 471, 560, 788, 887
MboI GATC 5 cut(s) 359, 469, 558, 786, 885
MboII GAAGA 3 cut(s) 120, 724, 796
MfeI CAATTG 1 cut(s) 653
MflI RGATCY 2 cut(s) 359, 469
MhlI GDGCHC 1 cut(s) 628
MlsI TGGCCA 2 cut(s) 220, 348
MluNI TGGCCA 2 cut(s) 220, 348
MlyI GAGTC 1 cut(s) 601
MnlI CCTC 5 cut(s) 206, 290, 545, 642, 970
Mox20I TGGCCA 2 cut(s) 220, 348
Mph1103I ATGCAT 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 127
MscI TGGCCA 2 cut(s) 220, 348
MseI TTAA 3 cut(s) 297, 1149, 1212
MslI CAYNNNNRTG 1 cut(s) 542
Msp20I TGGCCA 2 cut(s) 220, 348
MspR9I CCNGG 3 cut(s) 846, 946, 1089
MunI CAATTG 1 cut(s) 653
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 3 cut(s) 846, 946, 1089
MwoI GCNNNNNNNGC 5 cut(s) 254, 460, 513, 525, 623
NdeI CATATG 1 cut(s) 37
NdeII GATC 5 cut(s) 359, 469, 558, 786, 885
NlaIII CATG 3 cut(s) 159, 547, 1206
NlaIV GGNNCC 1 cut(s) 951
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 159
NspV TTCGAA 2 cut(s) 1172, 1217
PaeR7I CTCGAG 1 cut(s) 378
PciI ACATGT 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 971
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 1007, 1144
PkrI GCNGC 6 cut(s) 414, 417, 518, 616, 923, 1021
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
Ppu21I YACGTR 1 cut(s) 749
PpuMI RGGWCCY 1 cut(s) 803
PscI ACATGT 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 803
Psp6I CCWGG 3 cut(s) 844, 944, 1087
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 3 cut(s) 844, 944, 1087
PspN4I GGNNCC 1 cut(s) 951
PspPI GGNCC 1 cut(s) 803
PspPPI RGGWCCY 1 cut(s) 803
PstNI CAGNNNCTG 1 cut(s) 934
PsuI RGATCY 2 cut(s) 359, 469
RsaI GTAC 5 cut(s) 7, 596, 943, 1067, 1207
RsaNI GTAC 5 cut(s) 6, 595, 942, 1066, 1206
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 3 cut(s) 297, 1149, 1212
SatI GCNGC 6 cut(s) 413, 416, 517, 615, 922, 1020
Sau3AI GATC 5 cut(s) 359, 469, 558, 786, 885
Sau96I GGNCC 1 cut(s) 803
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 3 cut(s) 846, 946, 1089
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 4 cut(s) 152, 298, 310, 870
SfcI CTRYAG 1 cut(s) 407
Sfr274I CTCGAG 1 cut(s) 378
SfuI TTCGAA 2 cut(s) 1172, 1217
SinI GGWCC 1 cut(s) 803
SlaI CTCGAG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 2 cut(s) 378, 609
SmoI CTYRAG 2 cut(s) 378, 609
SnaBI TACGTA 1 cut(s) 749
SsiI CCGC 2 cut(s) 97, 516
StyD4I CCNGG 3 cut(s) 844, 944, 1087
StyI CCWWGG 4 cut(s) 177, 641, 1001, 1129
TaaI ACNGT 4 cut(s) 930, 1044, 1058, 1065
TaiI ACGT 2 cut(s) 751, 828
TaqI TCGA 7 cut(s) 379, 561, 619, 686, 1075, 1172, 1217
TatI WGTACW 2 cut(s) 594, 1205
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 2 cut(s) 1007, 1144
Tru1I TTAA 3 cut(s) 297, 1149, 1212
Tru9I TTAA 3 cut(s) 297, 1149, 1212
TscAI CASTG 4 cut(s) 575, 1113, 1146, 1184
TseI GCWGC 5 cut(s) 412, 415, 614, 921, 1019
TspDTI ATGAA 5 cut(s) 7, 24, 560, 685, 725
TspRI CASTG 4 cut(s) 575, 1113, 1146, 1184
VpaK11BI GGWCC 1 cut(s) 803
XagI CCTNNNNNAGG 1 cut(s) 798
XapI RAATTY 4 cut(s) 816, 967, 987, 1188
XbaI TCTAGA 3 cut(s) 356, 991, 1048
XceI RCATGY 1 cut(s) 159
XhoI CTCGAG 1 cut(s) 378
XmiI GTMKAC 3 cut(s) 160, 403, 1060
XmnI GAANNNNTTC 1 cut(s) 127
ZrmI AGTACT 1 cut(s) 596
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.