Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000250

Physical Location & Seq

Genomic Coordinates Forward (+)

36787874 .. 36790531

Gene Structure 2658 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G024770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGCCTTTCGGGTTAAAAAATGCAGGTGCCACGTATCAGCGACTCGTCAACTCCATGTTTGAGGAAGTTATCGGTACTATCATGGAGGTATACGTGGACGACATGCTGGTAAAAAGTCTAACTCTGGAGGATCATGTAGCCAACTTGTCAATCGTCTTTACCATCATATTGCGCAACGGGATGCGGCTTAACCCACAGAAGTGCATCTTCGGGGTCGAGGCCGGAAAGTTCCTCGGGTACATCATCAGCCACAAGGGAATTGAGGCCAATCCAGAGAAAGTGCAGGCTATAATAGACATGGTATCCCCAACCTACCGGAACGAGGTCCAATCTCTTGTAGGCAAAGTGGCAGCCCTGTCAAGATTCATCTCCAGGCTGACGGACAAGTGTACTCCGTTCTTCAAACTGCTAAAAACCCAACACGTTGAGGTGATAGCCTGGACAGTGGAGCACGAGGCTGCTTTCCTTTGCTTGAATTCAGTCCTGATTAGGAAGGACTCTGATTGCGAGTACCCAGTGTACTACGCCGGGAAAGGTTACACTGGTGCAGAGTCCAAGTACTCGGACATTGAAAGAGTAGCACTGGCCCTCCTGGTATCAGCCCGGAAGCTCAGACATTACTTCCAAGCACATTCTATCACCGTTTTCACTAATCACCCATTGAGGCAGGTTTTGCAGAAACCAGAGACATCGGGCAGGTTAATTAAATGGGCCATCGAGCTTGGGGAATTCGATATCAAGTACCTGCCCCGGACAGCCATTAAAGGCCAGGCAGAGGACGATTTTATTTCCGAATCAATCCCTTCCCATGACCCAAACAAGGAACCACCTGAACCGCTAGCCTCGGATCCACCTCCGCCAAGCATTTGGAGATTATACGTGGACGGATCATCCAACAAGAAAACTAGCGGAGCCGGCATCCTACTAATTAGCCCGGACGAGCAAGTCTACGAGTACGCCCTCAAATTTGCCTTCAAAGCATCCAATAATACCGCAGAATACGAGGCACTCATAGCCCGTCTGCAGATCTCCCGGGAACTAGGGGTCCAACACCTCCATATCTTCAGTGATTCCCAGTTGGTCGTAAACCAGGTCAGCGGTAACTTCGAGGCAAAGGAGCCCCACATGTCCTCCTACCAGGCCTTGGCCCGGGCTTTAGTTCAGAGGTTCACCTCCTACATTTTTACCCAAATACCGAGGGCAGAAAACGACAAGGCAAACGCTCTTGCTAAGCTCGCATCAACTTCTCCAAACCCTACCTATGGGACCACTAAAGTCGAAATACTCGCGGGACCTAGCACTTCCAAAACGGTGTTAGAAATATTTTCGGTGGATCACAAAGCTTCATGGATGGACCCAATTTTGAAATACATGGTTGATGGCCTAGCACCGGATGATAAGGTCGAGGCCAGAAGACTGCAGCTAAGATCAGCTCGATACACGATCATGAATGGCAAACTCTACAGAAGAGGACATTGCTTTCCCAACCTGAAGTGTGTGACACCGGAGGAAGGTCACAAAATAATGACGGACATTCACGCTGGTGTATGTGGTAACCATGCCGGAGCTCGATCTCTTGCACACAAGACCCTAAGGGCAGGATATTTTTGGCCAACCATGTCGGCCCTGGCTCAAACTATATCAACTTCTTGCCACAAATGCCAAATGTACGCAAATATCCCACGAGCACCGCCCATCGCCCTTTCCATCCTCCTTGCACCATGGCCGTTCTGTCAGTGGGGTTTGGACTTGATTGGTAAACTTCCCACAGCAGTGGGACAGTTCAAATACGCCATTGTTGCCGTGGACTACCAAACAAAGTGGGTAGAGGCAGAACCTCTCGTCGCCATCACCACGGAAAAAGTCAAGAACTTCCTATGGAAGAACATCTACTGCAGGTTTGGAGTCCCGGATACCATAGTTACGGACAATGGTACCCAGTTTGACAATGATGAGTTGAGGGCTTACACACAAAATCTCGGCACTAAGATCCTCTATGCTTCCCCGGCTCACCCCCAGACAAACGGTCAGGTCGAAGCCATCAACAAGATAATCAAGAAAATACTGAAAAAGAAGCTTGATGAAGCCAAGGGTCTTTGGGCCGCTAAACTCCCGGAAGTGTTGTGGGCTATCAGAACCACGGCAACGGAGGCCACGGGCGAGACCCCTTTCTGCATGGCTTTTGGATCAGAAGCAGTACTCCCTATTGAAACACAAGTCCAGAGTCCCCGGATCGAATACTTCGATGCGGGTACCAACTCAGATGGCCTACACCTCGATGCTGATTTACTCGAGGAACGAAGAGAAGTGGCACATATGCATAACTTGGCAAACAAGCGGAGGATAGCTCGTTACTACGATGCCAAGGTACAGTCCCGGACATTAAAATTAGGGGACTGGGTCATGAAGCAAGTCTACCCAGAACCCCGGGGACTGGATCCATCCTGGATAGGGCCTTACGAGATTATTGAAGAGGTAGGTCCCGCAACCTTTTTCATCCGGGATATGAACGGGGTCGTTTCCAGGCATCCATGGAATACCCAGCGCCTGTGGTACTATTACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000943 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003887 GO:0003964 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006278 GO:0006508 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0019538 GO:0032196 GO:0032197 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140096 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RVT_1 RVT_3 rve

Protein Analysis

855

Amino Acids

96.19

Weight (kDa)

8.53

Isoelectric Point (pI)

39.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_1	PF00078	1 - 82	6e-15	Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH	PF17917	150 - 244	2.5e-15	RNase H-like domain found in reverse transcriptase
RT_RNaseH_2	PF17919	150 - 215	4.7e-07	RNase H-like domain found in reverse transcriptase
RNase_H	PF00075	292 - 368	7.9e-08	RNase H
RVT_3	PF13456	294 - 413	1.6e-18	Reverse transcriptase-like
Integrase_H2C2	PF17921	502 - 557	5.3e-07	Integrase zinc binding domain
rve	PF00665	579 - 672	5e-11	Integrase core domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000250)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g22373 FvH4_1g22374 FvH4_1g25831 FvH4_1g28031 FvH4_2g11354 FvH4_3g11301 FvH4_3g11302 FvH4_3g14364 FvH4_3g20862 FvH4_4g15482 FvH4_4g15483 FvH4_4g15484 FvH4_5g25502 FvH4_5g37242 FvH4_6g18432 FvH4_6g18433 FvH4_6g20952 FvH4_7g02201 FvH4_7g10991
prunus_persica	Prupe.6G166700_v2.0.a1
pyrus_communis	pycom01g03860 pycom02g16130 pycom05g08340 pycom07g08280 pycom16g20980 pycom16g25810
rosa_chinensis	RchiOBHm_Chr5g0018261
rosa_laevigata	RLG00000022447
rosa_multiflora	Rmu_co7963284.1_g000001 Rmu_co7968088.1_g000001 Rmu_co8036800.1_g000001 Rmu_co8110294.1_g000001 Rmu_co8291955.1_g000001 Rmu_co8396209.1_g000002 Rmu_co8413879.1_g000001 Rmu_co8464165.1_g000001 Rmu_co8469565.1_g000001 Rmu_co8485695.1_g000003 Rmu_co8486821.1_g000003 Rmu_co8515379.1_g000001 Rmu_sc0000239.1_g000074 Rmu_sc0000327.1_g000028 Rmu_sc0000509.1_g000002 Rmu_sc0000677.1_g000042 Rmu_sc0000756.1_g000032 Rmu_sc0000782.1_g000013 Rmu_sc0001144.1_g000044 Rmu_sc0001861.1_g000003 Rmu_sc0002371.1_g000016 Rmu_sc0002451.1_g000061 Rmu_sc0002764.1_g000053 Rmu_sc0002868.1_g000041 Rmu_sc0003735.1_g000026 Rmu_sc0004654.1_g000006 Rmu_sc0005038.1_g000020 Rmu_sc0005236.1_g000001 Rmu_sc0006725.1_g000004 Rmu_sc0010685.1_g000001 Rmu_sc0011973.1_g000010 Rmu_sc0012722.1_g000001 Rmu_sc0015925.1_g000002 Rmu_sc0017188.1_g000001 Rmu_sc0021806.1_g000001 Rmu_sc0033125.1_g000001 Rmu_sc0038204.1_g000001 Rmu_ssc0000215.1_g000038
rosa_roxburghii	Rroxscaffold_1G00001020 Rroxscaffold_1G00027520 Rroxscaffold_3G00228140 Rroxscaffold_4G00320850 Rroxscaffold_4G00321830 Rroxscaffold_4G00321840 Rroxscaffold_4G00332450 Rroxscaffold_5G00344360 Rroxscaffold_5G00365190 Rroxscaffold_5G00377320 Rroxscaffold_5G00377330 Rroxscaffold_7G00183080 Rroxscaffold_7G00184560 Rroxscaffold_7G00216010
rosa_rugosa	Rorug02G0381000
rosa_samantha	Rh1AG155000 Rh1AG167000 Rh2AG095000 Rh3BG335500 Rh4AG082900 Rh4CG090200 Rh5CG445500 Rh7DG403200
rosa_wichuraiana	Rw0G001000 Rw0G014090 Rw0G019800 Rw0G020130 Rw0G020350 Rw1G002560 Rw1G013310 Rw1G014300 Rw1G014670 Rw1G016610 Rw1G021110 Rw1G024950 Rw1G028180 Rw2G020910 Rw2G024170 Rw2G024770 Rw2G029990 Rw2G031960 Rw2G034100 Rw3G013950 Rw3G028980 Rw3G029200 Rw4G004150 Rw4G008130 Rw4G012200 Rw4G018750 Rw4G019330 Rw4G021010 Rw4G022120 Rw4G030810 Rw5G015840 Rw5G015850 Rw5G022430 Rw5G022480 Rw5G026870 Rw5G033550 Rw6G009170 Rw6G010190 Rw6G011570 Rw6G018280 Rw6G023730 Rw7G025660 Rw7G029890 Rw7G031130 Rw7G042550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	14
AasI	GACNNNNNNGTC	1 cut(s)	944
Acc16I	TGCGCA	1 cut(s)	173
Acc36I	ACCTGC	5 cut(s)	14, 658, 687, 753, 1887
Acc65I	GGTACC	2 cut(s)	1934, 2252
AccB1I	GGYRCC	3 cut(s)	26, 1934, 2252
AccB7I	CCANNNNNTGG	1 cut(s)	1144
AccI	GTMKAC	3 cut(s)	90, 948, 2415
AccII	CGCG	1 cut(s)	1289
AcoI	YGGCCR	2 cut(s)	1610, 1724
AcsI	RAATTY	3 cut(s)	475, 728, 965
AcuI	CTGAAG	2 cut(s)	1048, 1511
AflIII	ACRYGT	2 cut(s)	421, 1125
AgsI	TTSAA	8 cut(s)	403, 475, 572, 976, 1366, 1786, 2210, 2471
AhdI	GACNNNNNGTC	1 cut(s)	2025
AjnI	CCWGG	9 cut(s)	371, 437, 591, 768, 1089, 1137, 1626, 2444, 2522
AleI	CACNNNNGTG	3 cut(s)	199, 1542, 1772
AloI	GAACNNNNNNTCC	2 cut(s)	1029, 1061
AluBI	AGCT	9 cut(s)	610, 721, 1234, 1343, 1423, 1433, 1568, 2077, 2348
AluI	AGCT	9 cut(s)	610, 721, 1234, 1343, 1423, 1433, 1568, 2077, 2348
Alw21I	GWGCWC	3 cut(s)	453, 1570, 1690
Alw26I	GTCTC	2 cut(s)	680, 2156
AlwNI	CAGNNNCTG	1 cut(s)	2548
Ama87I	CYCGRG	5 cut(s)	233, 1032, 1149, 2291, 2427
ApeKI	GCWGC	3 cut(s)	350, 458, 1420
ApoI	RAATTY	3 cut(s)	475, 728, 965
Asp718I	GGTACC	2 cut(s)	1934, 2252
AspLEI	GCGC	2 cut(s)	174, 2547
AsuHPI	GGTGA	6 cut(s)	442, 631, 647, 1162, 1843, 2003
AsuNHI	GCTAGC	1 cut(s)	838
AvaI	CYCGRG	5 cut(s)	233, 1032, 1149, 2291, 2427
AvaII	GGWCC	6 cut(s)	325, 1045, 1266, 1292, 1354, 2480
AxyI	CCTNAGG	1 cut(s)	1592
BaeI	ACNNNNGTAYC	4 cut(s)	1905, 1918, 1938, 1951
BalI	TGGCCA	1 cut(s)	1612
BamHI	GGATCC	2 cut(s)	847, 2437
BanI	GGYRCC	3 cut(s)	26, 1934, 2252
BanII	GRGCYC	2 cut(s)	1122, 1570
BarI	GAAGNNNNNNTAC	4 cut(s)	1874, 1906, 2399, 2431
BauI	CACGAG	2 cut(s)	452, 1683
BbsI	GAAGAC	1 cut(s)	1420
Bbv12I	GWGCWC	3 cut(s)	453, 1570, 1690
BbvI	GCAGC	3 cut(s)	362, 445, 1432
BceAI	ACGGC	3 cut(s)	1711, 1787, 2157
BciT130I	CCWGG	9 cut(s)	373, 439, 593, 770, 1091, 1139, 1628, 2446, 2524
BciVI	GTATCC	2 cut(s)	313, 1906
BcoDI	GTCTC	2 cut(s)	680, 2156
BfaI	CTAG	5 cut(s)	839, 906, 1040, 1296, 1385
BfmI	CTRYAG	4 cut(s)	1022, 1418, 1462, 1894
BfoI	RGCGCY	1 cut(s)	2548
BfuAI	ACCTGC	5 cut(s)	14, 658, 687, 753, 1887
BfuI	GTATCC	2 cut(s)	313, 1906
BglII	AGATCT	1 cut(s)	1026
BisI	GCNGC	5 cut(s)	185, 351, 459, 1421, 2103
BlpI	GCTNAGC	1 cut(s)	1230
BlsI	GCNGC	5 cut(s)	186, 352, 460, 1422, 2104
BmcAI	AGTACT	2 cut(s)	560, 2199
Bme18I	GGWCC	6 cut(s)	325, 1045, 1266, 1292, 1354, 2480
BmeRI	GACNNNNNGTC	1 cut(s)	2025
BmeT110I	CYCGRG	5 cut(s)	233, 1032, 1149, 2291, 2427
BmrI	ACTGGG	4 cut(s)	509, 1069, 1933, 2407
BmsI	GCATC	9 cut(s)	171, 213, 927, 989, 1247, 2236, 2269, 2350, 2536
BmtI	GCTAGC	1 cut(s)	842
BmuI	ACTGGG	4 cut(s)	509, 1069, 1933, 2407
BpiI	GAAGAC	1 cut(s)	1420
BplI	GAGNNNNNCTC	2 cut(s)	2332, 2364
BpmI	CTGGAG	2 cut(s)	146, 355
Bpu1102I	GCTNAGC	1 cut(s)	1230
BsaAI	YACGTR	3 cut(s)	33, 94, 880
BsaI	GGTCTC	1 cut(s)	2156
BsaWI	WCCGGW	3 cut(s)	315, 1390, 1504
BsaXI	ACNNNNNCTCC	4 cut(s)	573, 603, 1115, 1145
Bse118I	RCCGGY	1 cut(s)	914
Bse1I	ACTGG	7 cut(s)	515, 547, 588, 1075, 1939, 2402, 2439
Bse21I	CCTNAGG	1 cut(s)	1592
Bse3DI	GCAATG	1 cut(s)	1474
BseBI	CCWGG	9 cut(s)	373, 439, 593, 770, 1091, 1139, 1628, 2446, 2524
BseMI	GCAATG	1 cut(s)	1474
BseMII	CTCAG	2 cut(s)	625, 2274
BseNI	ACTGG	7 cut(s)	515, 547, 588, 1075, 1939, 2402, 2439
BseXI	GCAGC	3 cut(s)	362, 445, 1432
BseYI	CCCAGC	1 cut(s)	2541
BsgI	GTGCAG	2 cut(s)	302, 567
Bsh1236I	CGCG	1 cut(s)	1289
BshNI	GGYRCC	3 cut(s)	26, 1934, 2252
BsiHKAI	GWGCWC	3 cut(s)	453, 1570, 1690
BsiHKCI	CYCGRG	5 cut(s)	233, 1032, 1149, 2291, 2427
BslFI	GGGAC	9 cut(s)	1279, 1305, 1791, 1892, 2211, 2359, 2408, 2445, 2466
BsmAI	GTCTC	2 cut(s)	680, 2156
BsmFI	GGGAC	9 cut(s)	1279, 1305, 1791, 1892, 2211, 2359, 2408, 2445, 2466
Bso31I	GGTCTC	1 cut(s)	2156
BsoBI	CYCGRG	5 cut(s)	233, 1032, 1149, 2291, 2427
Bsp1286I	GDGCHC	4 cut(s)	453, 1122, 1570, 1690
Bsp1720I	GCTNAGC	1 cut(s)	1230
Bsp19I	CCATGG	2 cut(s)	1721, 2531
BspCNI	CTCAG	2 cut(s)	624, 2273
BspFNI	CGCG	1 cut(s)	1289
BspHI	TCATGA	2 cut(s)	1446, 2403
BspMAI	CTGCAG	3 cut(s)	1026, 1422, 1898
BspMI	ACCTGC	5 cut(s)	14, 658, 687, 753, 1887
BspOI	GCTAGC	1 cut(s)	842
BspT107I	GGYRCC	3 cut(s)	26, 1934, 2252
BspTNI	GGTCTC	1 cut(s)	2156
BsrDI	GCAATG	1 cut(s)	1474
BsrFI	RCCGGY	1 cut(s)	914
BsrI	ACTGG	7 cut(s)	515, 547, 588, 1075, 1939, 2402, 2439
BssAI	RCCGGY	1 cut(s)	914
BssNAI	GTATAC	1 cut(s)	91
BssSI	CACGAG	2 cut(s)	452, 1683
BssT1I	CCWWGG	5 cut(s)	1143, 1721, 2088, 2364, 2531
Bst1107I	GTATAC	1 cut(s)	91
Bst2BI	CACGAG	2 cut(s)	452, 1683
Bst2UI	CCWGG	9 cut(s)	373, 439, 593, 770, 1091, 1139, 1628, 2446, 2524
Bst4CI	ACNGT	6 cut(s)	445, 643, 1312, 1782, 2027, 2373
Bst6I	CTCTTC	3 cut(s)	1462, 2296, 2466
BstAPI	GCANNNNNTGC	1 cut(s)	673
BstBAI	YACGTR	3 cut(s)	33, 94, 880
BstC8I	GCNNGC	4 cut(s)	285, 840, 916, 1236
BstDEI	CTNAG	6 cut(s)	611, 1230, 1424, 1592, 1986, 2260
BstDSI	CCRYGG	6 cut(s)	1721, 1803, 1854, 2139, 2154, 2531
BstEII	GGTNACC	1 cut(s)	1553
BstFNI	CGCG	1 cut(s)	1289
BstH2I	RGCGCY	1 cut(s)	2548
BstHHI	GCGC	2 cut(s)	174, 2547
BstMAI	GTCTC	2 cut(s)	680, 2156
BstMWI	GCNNNNNNNGC	9 cut(s)	673, 915, 977, 1013, 1235, 1659, 1799, 2006, 2150
BstNI	CCWGG	9 cut(s)	373, 439, 593, 770, 1091, 1139, 1628, 2446, 2524
BstNSI	RCATGY	2 cut(s)	106, 1129
BstPI	GGTNACC	1 cut(s)	1553
BstSFI	CTRYAG	4 cut(s)	1022, 1418, 1462, 1894
BstUI	CGCG	1 cut(s)	1289
BstV1I	GCAGC	3 cut(s)	362, 445, 1432
BstV2I	GAAGAC	1 cut(s)	1420
BstX2I	RGATCY	4 cut(s)	847, 1026, 1989, 2437
BstXI	CCANNNNNNTGG	2 cut(s)	867, 1774
BstYI	RGATCY	4 cut(s)	847, 1026, 1989, 2437
BstZ17I	GTATAC	1 cut(s)	91
Bsu36I	CCTNAGG	1 cut(s)	1592
BsuI	GTATCC	2 cut(s)	313, 1906
BtgI	CCRYGG	6 cut(s)	1721, 1803, 1854, 2139, 2154, 2531
BtgZI	GCGATG	1 cut(s)	1681
BtsI	GCAGTG	1 cut(s)	1779
BtsIMutI	CAGTG	7 cut(s)	450, 522, 540, 581, 1072, 1742, 1779
BveI	ACCTGC	5 cut(s)	14, 658, 687, 753, 1887
Cac8I	GCNNGC	4 cut(s)	285, 840, 916, 1236
CaiI	CAGNNNCTG	1 cut(s)	2548
CciI	TCATGA	2 cut(s)	1446, 2403
CfoI	GCGC	2 cut(s)	174, 2547
Cfr10I	RCCGGY	1 cut(s)	914
Cfr9I	CCCGGG	3 cut(s)	1032, 1149, 2427
CsiI	ACCWGGT	1 cut(s)	1089
CspCI	CAANNNNNGTGG	2 cut(s)	1802, 1837
DdeI	CTNAG	6 cut(s)	611, 1230, 1424, 1592, 1986, 2260
DrdI	GACNNNNNNGTC	1 cut(s)	944
DriI	GACNNNNNGTC	1 cut(s)	2025
DseDI	GACNNNNNNGTC	1 cut(s)	944
EaeI	YGGCCR	2 cut(s)	1610, 1724
Eam1104I	CTCTTC	3 cut(s)	1462, 2296, 2466
Eam1105I	GACNNNNNGTC	1 cut(s)	2025
EarI	CTCTTC	3 cut(s)	1462, 2296, 2466
EciI	GGCGGA	1 cut(s)	846
Ecl136II	GAGCTC	1 cut(s)	1568
Eco130I	CCWWGG	5 cut(s)	1143, 1721, 2088, 2364, 2531
Eco147I	AGGCCT	1 cut(s)	1142
Eco24I	GRGCYC	2 cut(s)	1122, 1570
Eco31I	GGTCTC	1 cut(s)	2156
Eco32I	GATATC	1 cut(s)	736
Eco47I	GGWCC	6 cut(s)	325, 1045, 1266, 1292, 1354, 2480
Eco53kI	GAGCTC	1 cut(s)	1568
Eco57I	CTGAAG	2 cut(s)	1048, 1511
Eco81I	CCTNAGG	1 cut(s)	1592
Eco88I	CYCGRG	5 cut(s)	233, 1032, 1149, 2291, 2427
Eco91I	GGTNACC	1 cut(s)	1553
EcoICRI	GAGCTC	1 cut(s)	1568
EcoO109I	RGGNCCY	3 cut(s)	1292, 2453, 2480
EcoO65I	GGTNACC	1 cut(s)	1553
EcoRI	GAATTC	2 cut(s)	475, 728
EcoRII	CCWGG	9 cut(s)	371, 437, 591, 768, 1089, 1137, 1626, 2444, 2522
EcoRV	GATATC	1 cut(s)	736
EcoT14I	CCWWGG	5 cut(s)	1143, 1721, 2088, 2364, 2531
EcoT22I	ATGCAT	1 cut(s)	2322
EcoT38I	GRGCYC	2 cut(s)	1122, 1570
ErhI	CCWWGG	5 cut(s)	1143, 1721, 2088, 2364, 2531
FalI	AAGNNNNNCTT	2 cut(s)	191, 223
FaqI	GGGAC	9 cut(s)	1279, 1305, 1791, 1892, 2211, 2359, 2408, 2445, 2466
FauI	CCCGC	3 cut(s)	1282, 2242, 2491
FauNDI	CATATG	1 cut(s)	2316
FblI	GTMKAC	3 cut(s)	90, 948, 2415
Fnu4HI	GCNGC	5 cut(s)	185, 351, 459, 1421, 2103
FriOI	GRGCYC	2 cut(s)	1122, 1570
Fsp4HI	GCNGC	5 cut(s)	185, 351, 459, 1421, 2103
FspBI	CTAG	5 cut(s)	839, 906, 1040, 1296, 1385
FspI	TGCGCA	1 cut(s)	173
GlaI	GCGC	2 cut(s)	173, 2546
GluI	GCNGC	5 cut(s)	185, 351, 459, 1421, 2103
GsaI	CCCAGC	1 cut(s)	2545
GsuI	CTGGAG	2 cut(s)	146, 355
HaeII	RGCGCY	1 cut(s)	2548
HhaI	GCGC	2 cut(s)	174, 2547
Hin6I	GCGC	2 cut(s)	172, 2545
HinP1I	GCGC	2 cut(s)	172, 2545
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	2 cut(s)	1341, 2075
HinfI	GANTC	8 cut(s)	42, 363, 497, 551, 794, 1070, 1905, 2224
HphI	GGTGA	6 cut(s)	442, 631, 647, 1162, 1843, 2003
Hpy188I	TCNGA	9 cut(s)	502, 565, 614, 793, 847, 1164, 2135, 2191, 2263
Hpy188III	TCNNGA	9 cut(s)	125, 272, 360, 484, 1447, 1867, 2056, 2221, 2404
Hpy99I	CGWCG	1 cut(s)	1847
HpyAV	CCTTC	4 cut(s)	487, 813, 982, 1505
HpyCH4III	ACNGT	6 cut(s)	445, 643, 1312, 1782, 2027, 2373
HpyCH4IV	ACGT	4 cut(s)	32, 93, 423, 879
HpyF10VI	GCNNNNNNNGC	9 cut(s)	673, 915, 977, 1013, 1235, 1659, 1799, 2006, 2150
HpyF3I	CTNAG	6 cut(s)	611, 1230, 1424, 1592, 1986, 2260
HpySE526I	ACGT	4 cut(s)	32, 93, 423, 879
HspAI	GCGC	2 cut(s)	172, 2545
KpnI	GGTACC	2 cut(s)	1938, 2256
KroI	GCCGGC	1 cut(s)	914
KroNI	GCCGGC	1 cut(s)	916
LmnI	GCTCC	4 cut(s)	448, 911, 1117, 1565
Lsp1109I	GCAGC	3 cut(s)	362, 445, 1432
LweI	GCATC	9 cut(s)	171, 213, 927, 989, 1247, 2236, 2269, 2350, 2536
MabI	ACCWGGT	1 cut(s)	1089
MaeI	CTAG	5 cut(s)	839, 906, 1040, 1296, 1385
MaeII	ACGT	4 cut(s)	32, 93, 423, 879
MaeIII	GTNAC	7 cut(s)	536, 1100, 1498, 1514, 1553, 1921, 2351
MboII	GAAGA	8 cut(s)	199, 391, 1054, 1425, 1479, 1894, 2313, 2483
MflI	RGATCY	4 cut(s)	847, 1026, 1989, 2437
MhlI	GDGCHC	4 cut(s)	453, 1122, 1570, 1690
MlsI	TGGCCA	1 cut(s)	1612
MluCI	AATT	8 cut(s)	258, 475, 702, 728, 927, 965, 1359, 2387
MluNI	TGGCCA	1 cut(s)	1612
MlyI	GAGTC	5 cut(s)	36, 491, 560, 1914, 2233
MmeI	TCCRAC	2 cut(s)	918, 1072
Mox20I	TGGCCA	1 cut(s)	1612
Mph1103I	ATGCAT	1 cut(s)	2322
MroNI	GCCGGC	1 cut(s)	914
MscI	TGGCCA	1 cut(s)	1612
MseI	TTAA	6 cut(s)	14, 189, 701, 705, 762, 2384
MslI	CAYNNNNRTG	5 cut(s)	199, 1445, 1542, 1772, 2277
Msp20I	TGGCCA	1 cut(s)	1612
MspA1I	CMGCKG	1 cut(s)	1098
MvaI	CCWGG	9 cut(s)	373, 439, 593, 770, 1091, 1139, 1628, 2446, 2524
MvnI	CGCG	1 cut(s)	1289
MwoI	GCNNNNNNNGC	9 cut(s)	673, 915, 977, 1013, 1235, 1659, 1799, 2006, 2150
NaeI	GCCGGC	1 cut(s)	916
NcoI	CCATGG	2 cut(s)	1721, 2531
NdeI	CATATG	1 cut(s)	2316
NgoMIV	GCCGGC	1 cut(s)	914
NheI	GCTAGC	1 cut(s)	838
NmeAIII	GCCGAG	1 cut(s)	1959
NmuCI	GTSAC	2 cut(s)	1498, 1514
NsbI	TGCGCA	1 cut(s)	173
NsiI	ATGCAT	1 cut(s)	2322
NspI	RCATGY	2 cut(s)	106, 1129
OliI	CACNNNNGTG	3 cut(s)	199, 1542, 1772
PacI	TTAATTAA	1 cut(s)	705
PaeR7I	CTCGAG	1 cut(s)	2291
PagI	TCATGA	2 cut(s)	1446, 2403
PaqCI	CACCTGC	1 cut(s)	14
PceI	AGGCCT	1 cut(s)	1142
PciI	ACATGT	1 cut(s)	1125
PcsI	WCGNNNNNNNCGW	2 cut(s)	2031, 2241
PdiI	GCCGGC	1 cut(s)	916
PfeI	GAWTC	3 cut(s)	363, 794, 1070
PflFI	GACNNNGTC	1 cut(s)	2399
PflMI	CCANNNNNTGG	1 cut(s)	1144
PfoI	TCCNGGA	5 cut(s)	1908, 2112, 2375, 2444, 2499
PkrI	GCNGC	5 cut(s)	186, 352, 460, 1422, 2104
PleI	GAGTC	5 cut(s)	36, 491, 559, 1913, 2232
PpsI	GAGTC	5 cut(s)	36, 491, 559, 1913, 2232
Ppu21I	YACGTR	3 cut(s)	33, 94, 880
PpuMI	RGGWCCY	2 cut(s)	1292, 2480
PscI	ACATGT	1 cut(s)	1125
Psp124BI	GAGCTC	1 cut(s)	1570
Psp5II	RGGWCCY	2 cut(s)	1292, 2480
Psp6I	CCWGG	9 cut(s)	371, 437, 591, 768, 1089, 1137, 1626, 2444, 2522
PspEI	GGTNACC	1 cut(s)	1553
PspFI	CCCAGC	1 cut(s)	2541
PspGI	CCWGG	9 cut(s)	371, 437, 591, 768, 1089, 1137, 1626, 2444, 2522
PspPPI	RGGWCCY	2 cut(s)	1292, 2480
PspXI	VCTCGAGB	1 cut(s)	2291
PstI	CTGCAG	3 cut(s)	1026, 1422, 1898
PstNI	CAGNNNCTG	1 cut(s)	2548
PsuI	RGATCY	4 cut(s)	847, 1026, 1989, 2437
PsyI	GACNNNGTC	1 cut(s)	2399
RseI	CAYNNNNRTG	5 cut(s)	199, 1445, 1542, 1772, 2277
SacI	GAGCTC	1 cut(s)	1570
SaqAI	TTAA	6 cut(s)	14, 189, 701, 705, 762, 2384
SatI	GCNGC	5 cut(s)	185, 351, 459, 1421, 2103
ScaI	AGTACT	2 cut(s)	560, 2199
SchI	GAGTC	5 cut(s)	36, 491, 560, 1914, 2233
SduI	GDGCHC	4 cut(s)	453, 1122, 1570, 1690
SexAI	ACCWGGT	1 cut(s)	1089
SfaNI	GCATC	9 cut(s)	171, 213, 927, 989, 1247, 2236, 2269, 2350, 2536
SfcI	CTRYAG	4 cut(s)	1022, 1418, 1462, 1894
Sfr274I	CTCGAG	1 cut(s)	2291
SinI	GGWCC	6 cut(s)	325, 1045, 1266, 1292, 1354, 2480
SlaI	CTCGAG	1 cut(s)	2291
SmaI	CCCGGG	3 cut(s)	1034, 1151, 2429
SmiMI	CAYNNNNRTG	5 cut(s)	199, 1445, 1542, 1772, 2277
SmlI	CTYRAG	1 cut(s)	2291
SmoI	CTYRAG	1 cut(s)	2291
SrfI	GCCCGGGC	1 cut(s)	1151
Sse9I	AATT	8 cut(s)	258, 475, 702, 728, 927, 965, 1359, 2387
SseBI	AGGCCT	1 cut(s)	1142
SspI	AATATT	1 cut(s)	1323
SspMI	CTAG	5 cut(s)	839, 906, 1040, 1296, 1385
SstI	GAGCTC	1 cut(s)	1570
StuI	AGGCCT	1 cut(s)	1142
StyI	CCWWGG	5 cut(s)	1143, 1721, 2088, 2364, 2531
TaaI	ACNGT	6 cut(s)	445, 643, 1312, 1782, 2027, 2373
TaiI	ACGT	4 cut(s)	35, 96, 426, 882
TasI	AATT	8 cut(s)	258, 475, 702, 728, 927, 965, 1359, 2387
TatI	WGTACW	4 cut(s)	389, 519, 558, 2197
TauI	GCSGC	2 cut(s)	187, 2105
TfiI	GAWTC	3 cut(s)	363, 794, 1070
Tru1I	TTAA	6 cut(s)	14, 189, 701, 705, 762, 2384
Tru9I	TTAA	6 cut(s)	14, 189, 701, 705, 762, 2384
TscAI	CASTG	7 cut(s)	450, 522, 547, 588, 1072, 1742, 1779
TseFI	GTSAC	2 cut(s)	1498, 1514
TseI	GCWGC	3 cut(s)	350, 458, 1420
Tsp45I	GTSAC	2 cut(s)	1498, 1514
TspDTI	ATGAA	7 cut(s)	355, 1335, 1463, 2097, 2420, 2485, 2522
TspGWI	ACGGA	7 cut(s)	384, 395, 900, 1544, 1871, 1940, 2162
TspMI	CCCGGG	3 cut(s)	1032, 1149, 2427
TspRI	CASTG	7 cut(s)	450, 522, 547, 588, 1072, 1742, 1779
Tth111I	GACNNNGTC	1 cut(s)	2399
Van91I	CCANNNNNTGG	1 cut(s)	1144
VpaK11BI	GGWCC	6 cut(s)	325, 1045, 1266, 1292, 1354, 2480
XapI	RAATTY	3 cut(s)	475, 728, 965
XceI	RCATGY	2 cut(s)	106, 1129
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1624, 1801
XhoI	CTCGAG	1 cut(s)	2291
XmaI	CCCGGG	3 cut(s)	1032, 1149, 2427
XmiI	GTMKAC	3 cut(s)	90, 948, 2415
XspI	CTAG	5 cut(s)	839, 906, 1040, 1296, 1385
ZrmI	AGTACT	2 cut(s)	560, 2199
Zsp2I	ATGCAT	1 cut(s)	2322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.