Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000250

Physical Location & Seq

Genomic Coordinates Forward (+)

28681831 .. 28684488

Gene Structure 2658 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G020910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGCCTTTCGGGTTAAAGAATGCAGGTGCCACCTATCCACGACTCGTCAACTCCATGTTTGAGGAAGTTATCGGTACTATCATGGAGGTATACGTGGACGACATGCTGGTAAAAAGTTTAACTCAGGAGGATCATGTAGCCAACTTGTCAATCGTCTTCACCATCATATTGCGCAACGGGATGCGGCTTAACCCACAGAAGTGCATCTTCGGGGTCGAGGCCGGAAAGTTCCTCGGGTACATCATCAGCCACAGGGGAATTGAAGCCAATCCAGAGAAAGTGCAGGCTATAATTGACATGGTATCCCCAACCTACCGGAATGAGGTCCAGTCTCTTGTAGGCAAGGTGGCCGCCCTGTCAAGATTCATCTCCAGGCTGACGGACAAGTGTACCCCTTTCTTCAAATTGCTAAAAACCCAACACGACTCCGATTGTGAGTACCCGGTGTACTACGCAGGCAAAGGTTACACTGGTGCAGAGTTCAGGTACCCGGACATTGAAAGAGTAGCACTGGCCCTCCTGGTATCAGCCCGGAAGCTCAGACATTACTTCCAGGTACACTCTATCACCGTTTTCACTAATCACCCATTGAGGCAGGTTTTGCAGAAACCAGAGACATCGGGCAGATTAATTAAATGGGCCATCGAGCTGGGGGAATTCGATATCAAGTACCTGCCCCGTACAGCCATCAAGGGCCAGGCAGCAGCCGATTTTATTTCCAAATCAATCCCTTCCCATGACCCAAGCAGGGAACCACCTGAACCACTAGCTTCGGATCCACCTCCGCCAAGCACTTGGCGATTATACGTGGATGGATCATCCAACAAGAGAACTAGCGGAGCCGGCATCCTGCTAATTAGCCCGGACGAGCAAGTCTACGAGTACGCCCTCAAATTTGCCTTCAAAGCATCCAATAATACCGCAGAATACGAGGCACTCATAGCCGGTCTGCAGATCGCCCGGGAACTAGGGGTCCAGCACCTCCATATCTTCAGCGATTCCCAGTTGGTCGTAAACCAGGTCAGCGGTAACTTCGAGGCAAAGGAACCCCACATGTCCTCCTACCAGGCCTTGGCCCGGGCTTTAGTCCAGAGGTTCACCTCCTACATTTTTACCCAAATACCGAGGGCAGAAAACGACAAGGCAGACGCTCTTGCTAAGCTCGCATCAGCTTCTCCAAACCCTACCTATGGGACCACTAAAGTCGAAATACTCGAGGGACCTAGCACTTCCAAAACGGTGTCAGAAATATTTTCGGTGGATCACAAAGCTTCATGGATGGATCCAATTTTGAAATACATGGTTGATGGCCTAGCACCGGATGATAAGGTCGAGGCCAGAAGACTGCAGCTAAGGTCAGCTCGGTACACGATCATTAATGGCAAACTCTACAGGAGAGGACATTGCTTTCCCAACCTGAAGTGTGTGACACCGGAGGAAGGTCACAAAGTAATGACGGACATTCACGCTGGTGTATGTGGTAACCATGCCGGAGCCCGATCTCTTGCACACAAGACCCTAAGGGCAGGATATTTCTGGCCAACCATGTCGGCCCTGGCTCAAACTATATCCAGTTCTTGCCACAAATGCCAAATGTACGCAAATATCCCACGAGCACCGCCCATCGCCCTTTCCATCCTCCTTGCACCATGGCCGTTCTGTCAGTGGGGTTTTGACTTGATTGGTAAACTTCCCACAGCAGTGGGACAGTTCAAGTACGCCATTGTTGCCGTAGACTACCAAACAAAGTGGGTAGAGGCAGAACCTCTCGTCGCCATCACCACGGAAAAAGTCAAGAACTTCCTCTGGAAGAACATATACTGCAGGTTCGGAGTCCCGGATACCATAGTCACGGACAATGGTACACAGTTTGACAATGATGAGTTGAGGGCTTACACACAGAACCTCGGCACTAAGATCCTCTATGCTTCCCCGGCTCACCCCCAGACAAACGGTCAGGTCAAAGCTATCAACAAGATAATCAAGAAGATACTGAAAAAGAAGCTTGACGAAGCCAAGGGCCTTTGGGCCGCTAAACTCCCGGAAGTGCTATGGGCTATCAGGACAACGGCAACAGAGGCCACGGGCGAGACCCCTTTCTGCATGGCTTTTGGATCAGAAGCAGTACTCCCTATTGAAACACAAGTCCAGAGTCCCCGGATCGAATACTTCGACGCGGGTACCAACTCAGATGGCCTACACCTCGATGCTGATTTACTTGAGGAGCGAAGAGAAGTCGCACATATGCATAACTTGGCGAACAAGCGGAGGATAGCTCGTTACTACGATGCCAAGGTACAGTCCCGAACATTAAAATTGAGGGACTGGGTCATGAAGCAAGTCTACCCAGAACCCCGGGGATTGGATCCATCCTGGACAGGGCCTTACGAGATCATTGAAGAGGTAGGTCCCGCAACTTTTTTCATCCGGGATATGAACGGGGTCGTTTCCAGGCATCCATGGAATACCCAGCGCCTGCGGTACTATTACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000943 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003887 GO:0003964 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006278 GO:0006508 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0019538 GO:0032196 GO:0032197 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140096 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RVT_1 RVT_3 rve

Protein Analysis

831

Amino Acids

93.45

Weight (kDa)

8.93

Isoelectric Point (pI)

39.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_1	PF00078	1 - 82	6.1e-14	Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH	PF17917	139 - 220	9.2e-14	RNase H-like domain found in reverse transcriptase
RNase_H	PF00075	267 - 345	1.9e-08	RNase H
RVT_3	PF13456	270 - 389	2.8e-22	Reverse transcriptase-like
Integrase_H2C2	PF17921	478 - 533	6.2e-07	Integrase zinc binding domain
rve	PF00665	556 - 648	5.6e-11	Integrase core domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000250)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g22373 FvH4_1g22374 FvH4_1g25831 FvH4_1g28031 FvH4_2g11354 FvH4_3g11301 FvH4_3g11302 FvH4_3g14364 FvH4_3g20862 FvH4_4g15482 FvH4_4g15483 FvH4_4g15484 FvH4_5g25502 FvH4_5g37242 FvH4_6g18432 FvH4_6g18433 FvH4_6g20952 FvH4_7g02201 FvH4_7g10991
prunus_persica	Prupe.6G166700_v2.0.a1
pyrus_communis	pycom01g03860 pycom02g16130 pycom05g08340 pycom07g08280 pycom16g20980 pycom16g25810
rosa_chinensis	RchiOBHm_Chr5g0018261
rosa_laevigata	RLG00000022447
rosa_multiflora	Rmu_co7963284.1_g000001 Rmu_co7968088.1_g000001 Rmu_co8036800.1_g000001 Rmu_co8110294.1_g000001 Rmu_co8291955.1_g000001 Rmu_co8396209.1_g000002 Rmu_co8413879.1_g000001 Rmu_co8464165.1_g000001 Rmu_co8469565.1_g000001 Rmu_co8485695.1_g000003 Rmu_co8486821.1_g000003 Rmu_co8515379.1_g000001 Rmu_sc0000239.1_g000074 Rmu_sc0000327.1_g000028 Rmu_sc0000509.1_g000002 Rmu_sc0000677.1_g000042 Rmu_sc0000756.1_g000032 Rmu_sc0000782.1_g000013 Rmu_sc0001144.1_g000044 Rmu_sc0001861.1_g000003 Rmu_sc0002371.1_g000016 Rmu_sc0002451.1_g000061 Rmu_sc0002764.1_g000053 Rmu_sc0002868.1_g000041 Rmu_sc0003735.1_g000026 Rmu_sc0004654.1_g000006 Rmu_sc0005038.1_g000020 Rmu_sc0005236.1_g000001 Rmu_sc0006725.1_g000004 Rmu_sc0010685.1_g000001 Rmu_sc0011973.1_g000010 Rmu_sc0012722.1_g000001 Rmu_sc0015925.1_g000002 Rmu_sc0017188.1_g000001 Rmu_sc0021806.1_g000001 Rmu_sc0033125.1_g000001 Rmu_sc0038204.1_g000001 Rmu_ssc0000215.1_g000038
rosa_roxburghii	Rroxscaffold_1G00001020 Rroxscaffold_1G00027520 Rroxscaffold_3G00228140 Rroxscaffold_4G00320850 Rroxscaffold_4G00321830 Rroxscaffold_4G00321840 Rroxscaffold_4G00332450 Rroxscaffold_5G00344360 Rroxscaffold_5G00365190 Rroxscaffold_5G00377320 Rroxscaffold_5G00377330 Rroxscaffold_7G00183080 Rroxscaffold_7G00184560 Rroxscaffold_7G00216010
rosa_rugosa	Rorug02G0381000
rosa_samantha	Rh1AG155000 Rh1AG167000 Rh2AG095000 Rh3BG335500 Rh4AG082900 Rh4CG090200 Rh5CG445500 Rh7DG403200
rosa_wichuraiana	Rw0G001000 Rw0G014090 Rw0G019800 Rw0G020130 Rw0G020350 Rw1G002560 Rw1G013310 Rw1G014300 Rw1G014670 Rw1G016610 Rw1G021110 Rw1G024950 Rw1G028180 Rw2G020910 Rw2G024170 Rw2G024770 Rw2G029990 Rw2G031960 Rw2G034100 Rw3G013950 Rw3G028980 Rw3G029200 Rw4G004150 Rw4G008130 Rw4G012200 Rw4G018750 Rw4G019330 Rw4G021010 Rw4G022120 Rw4G030810 Rw5G015840 Rw5G015850 Rw5G022430 Rw5G022480 Rw5G026870 Rw5G033550 Rw6G009170 Rw6G010190 Rw6G011570 Rw6G018280 Rw6G023730 Rw7G025660 Rw7G029890 Rw7G031130 Rw7G042550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	14
AasI	GACNNNNNNGTC	1 cut(s)	872
Acc16I	TGCGCA	1 cut(s)	173
Acc36I	ACCTGC	4 cut(s)	14, 586, 681, 1815
Acc65I	GGTACC	2 cut(s)	486, 2180
AccB1I	GGYRCC	3 cut(s)	26, 486, 2180
AccB7I	CCANNNNNTGG	1 cut(s)	1072
AccI	GTMKAC	4 cut(s)	90, 876, 1734, 2343
AccII	CGCG	1 cut(s)	2177
AcoI	YGGCCR	3 cut(s)	348, 1538, 1652
AcsI	RAATTY	2 cut(s)	656, 893
AcuI	CTGAAG	2 cut(s)	976, 1439
AfiI	CCNNNNNNNGG	9 cut(s)	322, 748, 1072, 1077, 1190, 1318, 1439, 2362, 2379
AflIII	ACRYGT	1 cut(s)	1053
AgsI	TTSAA	8 cut(s)	263, 403, 500, 904, 1294, 1714, 2138, 2399
AhdI	GACNNNNNGTC	1 cut(s)	1953
AjnI	CCWGG	9 cut(s)	371, 519, 552, 696, 1017, 1065, 1554, 2372, 2450
AleI	CACNNNNGTG	3 cut(s)	199, 1470, 1700
AloI	GAACNNNNNNTCC	2 cut(s)	957, 989
Alw21I	GWGCWC	1 cut(s)	1618
Alw26I	GTCTC	3 cut(s)	336, 608, 2084
AlwNI	CAGNNNCTG	1 cut(s)	2476
Ama87I	CYCGRG	5 cut(s)	233, 960, 1077, 1214, 2355
ApeKI	GCWGC	3 cut(s)	701, 704, 1348
ApoI	RAATTY	2 cut(s)	656, 893
AseI	ATTAAT	2 cut(s)	629, 1377
Asp718I	GGTACC	2 cut(s)	486, 2180
AspLEI	GCGC	2 cut(s)	174, 2475
AsuHPI	GGTGA	6 cut(s)	151, 559, 575, 1090, 1771, 1931
AvaI	CYCGRG	5 cut(s)	233, 960, 1077, 1214, 2355
AvaII	GGWCC	5 cut(s)	325, 973, 1194, 1220, 2408
AxyI	CCTNAGG	1 cut(s)	1520
BaeI	ACNNNNGTAYC	6 cut(s)	373, 406, 1833, 1866, 2164, 2197
BalI	TGGCCA	1 cut(s)	1540
BamHI	GGATCC	3 cut(s)	775, 1282, 2365
BanI	GGYRCC	3 cut(s)	26, 486, 2180
BanII	GRGCYC	1 cut(s)	1498
BarI	GAAGNNNNNNTAC	4 cut(s)	1802, 1834, 2327, 2359
BauI	CACGAG	1 cut(s)	1611
BbsI	GAAGAC	2 cut(s)	148, 1348
Bbv12I	GWGCWC	1 cut(s)	1618
BbvI	GCAGC	3 cut(s)	713, 716, 1360
BceAI	ACGGC	3 cut(s)	1639, 1715, 2085
BciT130I	CCWGG	9 cut(s)	373, 521, 554, 698, 1019, 1067, 1556, 2374, 2452
BciVI	GTATCC	2 cut(s)	313, 1834
BcoDI	GTCTC	3 cut(s)	336, 608, 2084
BfaI	CTAG	5 cut(s)	767, 834, 968, 1224, 1313
BfmI	CTRYAG	4 cut(s)	950, 1346, 1390, 1822
BfoI	RGCGCY	1 cut(s)	2476
BfuAI	ACCTGC	4 cut(s)	14, 586, 681, 1815
BfuI	GTATCC	2 cut(s)	313, 1834
BisI	GCNGC	6 cut(s)	185, 351, 702, 705, 1349, 2031
BlpI	GCTNAGC	1 cut(s)	1158
BlsI	GCNGC	6 cut(s)	186, 352, 703, 706, 1350, 2032
BmcAI	AGTACT	1 cut(s)	2127
Bme18I	GGWCC	5 cut(s)	325, 973, 1194, 1220, 2408
BmeRI	GACNNNNNGTC	1 cut(s)	1953
BmeT110I	CYCGRG	5 cut(s)	233, 960, 1077, 1214, 2355
BmrI	ACTGGG	2 cut(s)	997, 2335
BmsI	GCATC	8 cut(s)	171, 213, 855, 917, 1175, 2197, 2278, 2464
BmuI	ACTGGG	2 cut(s)	997, 2335
BpiI	GAAGAC	2 cut(s)	148, 1348
BplI	GAGNNNNNCTC	2 cut(s)	2260, 2292
BpmI	CTGGAG	1 cut(s)	355
Bpu10I	CCTNAGC	1 cut(s)	1352
Bpu1102I	GCTNAGC	1 cut(s)	1158
BpuEI	CTTGAG	1 cut(s)	2240
BsaAI	YACGTR	2 cut(s)	94, 808
BsaI	GGTCTC	1 cut(s)	2084
BsaWI	WCCGGW	3 cut(s)	315, 1318, 1432
BsaXI	ACNNNNNCTCC	4 cut(s)	501, 531, 1043, 1073
Bsc4I	CCNNNNNNNGG	9 cut(s)	322, 748, 1072, 1077, 1190, 1318, 1439, 2362, 2379
Bse118I	RCCGGY	2 cut(s)	842, 944
Bse1I	ACTGG	6 cut(s)	328, 475, 516, 1003, 1572, 2330
Bse21I	CCTNAGG	1 cut(s)	1520
Bse3DI	GCAATG	1 cut(s)	1402
BseBI	CCWGG	9 cut(s)	373, 521, 554, 698, 1019, 1067, 1556, 2374, 2452
BseLI	CCNNNNNNNGG	9 cut(s)	322, 748, 1072, 1077, 1190, 1318, 1439, 2362, 2379
BseMI	GCAATG	1 cut(s)	1402
BseMII	CTCAG	3 cut(s)	137, 553, 2202
BseNI	ACTGG	6 cut(s)	328, 475, 516, 1003, 1572, 2330
BseRI	GAGGAG	1 cut(s)	2237
BseXI	GCAGC	3 cut(s)	713, 716, 1360
BseYI	CCCAGC	2 cut(s)	649, 2469
BsgI	GTGCAG	2 cut(s)	302, 495
Bsh1236I	CGCG	1 cut(s)	2177
BshNI	GGYRCC	3 cut(s)	26, 486, 2180
BsiHKAI	GWGCWC	1 cut(s)	1618
BsiHKCI	CYCGRG	5 cut(s)	233, 960, 1077, 1214, 2355
BslFI	GGGAC	8 cut(s)	1207, 1233, 1719, 1820, 2139, 2287, 2336, 2394
BslI	CCNNNNNNNGG	9 cut(s)	322, 748, 1072, 1077, 1190, 1318, 1439, 2362, 2379
BsmAI	GTCTC	3 cut(s)	336, 608, 2084
BsmFI	GGGAC	8 cut(s)	1207, 1233, 1719, 1820, 2139, 2287, 2336, 2394
BsmI	GAATGC	1 cut(s)	25
Bso31I	GGTCTC	1 cut(s)	2084
BsoBI	CYCGRG	5 cut(s)	233, 960, 1077, 1214, 2355
Bsp1286I	GDGCHC	2 cut(s)	1498, 1618
Bsp1720I	GCTNAGC	1 cut(s)	1158
Bsp19I	CCATGG	2 cut(s)	1649, 2459
BspCNI	CTCAG	3 cut(s)	136, 552, 2201
BspFNI	CGCG	1 cut(s)	2177
BspHI	TCATGA	1 cut(s)	2331
BspMAI	CTGCAG	3 cut(s)	954, 1350, 1826
BspMI	ACCTGC	4 cut(s)	14, 586, 681, 1815
BspT107I	GGYRCC	3 cut(s)	26, 486, 2180
BspTNI	GGTCTC	1 cut(s)	2084
BsrDI	GCAATG	1 cut(s)	1402
BsrFI	RCCGGY	2 cut(s)	842, 944
BsrI	ACTGG	6 cut(s)	328, 475, 516, 1003, 1572, 2330
BssAI	RCCGGY	2 cut(s)	842, 944
BssNAI	GTATAC	1 cut(s)	91
BssSI	CACGAG	1 cut(s)	1611
BssT1I	CCWWGG	5 cut(s)	1071, 1649, 2016, 2292, 2459
Bst1107I	GTATAC	1 cut(s)	91
Bst2BI	CACGAG	1 cut(s)	1611
Bst2UI	CCWGG	9 cut(s)	373, 521, 554, 698, 1019, 1067, 1556, 2374, 2452
Bst4CI	ACNGT	6 cut(s)	571, 1240, 1710, 1869, 1955, 2301
Bst6I	CTCTTC	2 cut(s)	2224, 2394
BstAPI	GCANNNNNTGC	1 cut(s)	601
BstBAI	YACGTR	2 cut(s)	94, 808
BstC8I	GCNNGC	5 cut(s)	285, 457, 844, 1164, 2477
BstDEI	CTNAG	7 cut(s)	123, 539, 1158, 1352, 1520, 1914, 2188
BstDSI	CCRYGG	4 cut(s)	1649, 1782, 2082, 2459
BstEII	GGTNACC	1 cut(s)	1481
BstFNI	CGCG	1 cut(s)	2177
BstH2I	RGCGCY	1 cut(s)	2476
BstHHI	GCGC	2 cut(s)	174, 2475
BstMAI	GTCTC	3 cut(s)	336, 608, 2084
BstMWI	GCNNNNNNNGC	9 cut(s)	601, 843, 905, 941, 1163, 1587, 1727, 1934, 2078
BstNI	CCWGG	9 cut(s)	373, 521, 554, 698, 1019, 1067, 1556, 2374, 2452
BstNSI	RCATGY	2 cut(s)	106, 1057
BstPI	GGTNACC	1 cut(s)	1481
BstSFI	CTRYAG	4 cut(s)	950, 1346, 1390, 1822
BstUI	CGCG	1 cut(s)	2177
BstV1I	GCAGC	3 cut(s)	713, 716, 1360
BstV2I	GAAGAC	2 cut(s)	148, 1348
BstX2I	RGATCY	4 cut(s)	775, 1282, 1917, 2365
BstXI	CCANNNNNNTGG	3 cut(s)	649, 795, 1702
BstYI	RGATCY	4 cut(s)	775, 1282, 1917, 2365
BstZ17I	GTATAC	1 cut(s)	91
Bsu36I	CCTNAGG	1 cut(s)	1520
BsuI	GTATCC	2 cut(s)	313, 1834
BtgI	CCRYGG	4 cut(s)	1649, 1782, 2082, 2459
BtgZI	GCGATG	1 cut(s)	1609
BtsI	GCAGTG	1 cut(s)	1707
BtsIMutI	CAGTG	4 cut(s)	468, 509, 1670, 1707
BveI	ACCTGC	4 cut(s)	14, 586, 681, 1815
Cac8I	GCNNGC	5 cut(s)	285, 457, 844, 1164, 2477
CaiI	CAGNNNCTG	1 cut(s)	2476
CciI	TCATGA	1 cut(s)	2331
CfoI	GCGC	2 cut(s)	174, 2475
Cfr10I	RCCGGY	2 cut(s)	842, 944
Cfr9I	CCCGGG	3 cut(s)	960, 1077, 2355
CseI	GACGC	2 cut(s)	1157, 2183
CsiI	ACCWGGT	1 cut(s)	1017
CspCI	CAANNNNNGTGG	2 cut(s)	1730, 1765
DdeI	CTNAG	7 cut(s)	123, 539, 1158, 1352, 1520, 1914, 2188
DrdI	GACNNNNNNGTC	1 cut(s)	872
DriI	GACNNNNNGTC	1 cut(s)	1953
DseDI	GACNNNNNNGTC	1 cut(s)	872
EaeI	YGGCCR	3 cut(s)	348, 1538, 1652
Eam1104I	CTCTTC	2 cut(s)	2224, 2394
Eam1105I	GACNNNNNGTC	1 cut(s)	1953
EarI	CTCTTC	2 cut(s)	2224, 2394
EciI	GGCGGA	1 cut(s)	774
Eco130I	CCWWGG	5 cut(s)	1071, 1649, 2016, 2292, 2459
Eco147I	AGGCCT	1 cut(s)	1070
Eco24I	GRGCYC	1 cut(s)	1498
Eco31I	GGTCTC	1 cut(s)	2084
Eco32I	GATATC	1 cut(s)	664
Eco47I	GGWCC	5 cut(s)	325, 973, 1194, 1220, 2408
Eco57I	CTGAAG	2 cut(s)	976, 1439
Eco81I	CCTNAGG	1 cut(s)	1520
Eco88I	CYCGRG	5 cut(s)	233, 960, 1077, 1214, 2355
Eco91I	GGTNACC	1 cut(s)	1481
EcoO109I	RGGNCCY	4 cut(s)	1220, 2020, 2381, 2408
EcoO65I	GGTNACC	1 cut(s)	1481
EcoRI	GAATTC	1 cut(s)	656
EcoRII	CCWGG	9 cut(s)	371, 519, 552, 696, 1017, 1065, 1554, 2372, 2450
EcoRV	GATATC	1 cut(s)	664
EcoT14I	CCWWGG	5 cut(s)	1071, 1649, 2016, 2292, 2459
EcoT22I	ATGCAT	1 cut(s)	2250
EcoT38I	GRGCYC	1 cut(s)	1498
ErhI	CCWWGG	5 cut(s)	1071, 1649, 2016, 2292, 2459
FalI	AAGNNNNNCTT	2 cut(s)	191, 223
FaqI	GGGAC	8 cut(s)	1207, 1233, 1719, 1820, 2139, 2287, 2336, 2394
FauI	CCCGC	2 cut(s)	2170, 2419
FauNDI	CATATG	1 cut(s)	2244
FblI	GTMKAC	4 cut(s)	90, 876, 1734, 2343
Fnu4HI	GCNGC	6 cut(s)	185, 351, 702, 705, 1349, 2031
FriOI	GRGCYC	1 cut(s)	1498
Fsp4HI	GCNGC	6 cut(s)	185, 351, 702, 705, 1349, 2031
FspBI	CTAG	5 cut(s)	767, 834, 968, 1224, 1313
FspI	TGCGCA	1 cut(s)	173
GlaI	GCGC	2 cut(s)	173, 2474
GluI	GCNGC	6 cut(s)	185, 351, 702, 705, 1349, 2031
GsaI	CCCAGC	2 cut(s)	653, 2473
GsuI	CTGGAG	1 cut(s)	355
HaeII	RGCGCY	1 cut(s)	2476
HgaI	GACGC	2 cut(s)	1157, 2183
HhaI	GCGC	2 cut(s)	174, 2475
Hin6I	GCGC	2 cut(s)	172, 2473
HinP1I	GCGC	2 cut(s)	172, 2473
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	2 cut(s)	1269, 2003
HinfI	GANTC	6 cut(s)	42, 363, 425, 998, 1833, 2152
HphI	GGTGA	6 cut(s)	151, 559, 575, 1090, 1771, 1931
Hpy188I	TCNGA	7 cut(s)	430, 542, 775, 1246, 1832, 2119, 2191
Hpy99I	CGWCG	2 cut(s)	1775, 2177
HpyAV	CCTTC	3 cut(s)	741, 910, 1433
HpyCH4III	ACNGT	6 cut(s)	571, 1240, 1710, 1869, 1955, 2301
HpyCH4IV	ACGT	2 cut(s)	93, 807
HpyF10VI	GCNNNNNNNGC	9 cut(s)	601, 843, 905, 941, 1163, 1587, 1727, 1934, 2078
HpyF3I	CTNAG	7 cut(s)	123, 539, 1158, 1352, 1520, 1914, 2188
HpySE526I	ACGT	2 cut(s)	93, 807
HspAI	GCGC	2 cut(s)	172, 2473
KpnI	GGTACC	2 cut(s)	490, 2184
KroI	GCCGGC	1 cut(s)	842
KroNI	GCCGGC	1 cut(s)	844
LmnI	GCTCC	3 cut(s)	839, 1493, 2224
Lsp1109I	GCAGC	3 cut(s)	713, 716, 1360
LweI	GCATC	8 cut(s)	171, 213, 855, 917, 1175, 2197, 2278, 2464
MabI	ACCWGGT	1 cut(s)	1017
MaeI	CTAG	5 cut(s)	767, 834, 968, 1224, 1313
MaeII	ACGT	2 cut(s)	93, 807
MaeIII	GTNAC	7 cut(s)	464, 1028, 1426, 1442, 1481, 1849, 2279
MboII	GAAGA	9 cut(s)	148, 199, 391, 982, 1353, 1822, 1999, 2241, 2411
MflI	RGATCY	4 cut(s)	775, 1282, 1917, 2365
MhlI	GDGCHC	2 cut(s)	1498, 1618
MlsI	TGGCCA	1 cut(s)	1540
MluCI	AATT	9 cut(s)	258, 291, 404, 630, 656, 855, 893, 1287, 2315
MluNI	TGGCCA	1 cut(s)	1540
MlyI	GAGTC	4 cut(s)	36, 419, 1842, 2161
MmeI	TCCRAC	1 cut(s)	846
Mox20I	TGGCCA	1 cut(s)	1540
Mph1103I	ATGCAT	1 cut(s)	2250
MroNI	GCCGGC	1 cut(s)	842
MscI	TGGCCA	1 cut(s)	1540
MseI	TTAA	7 cut(s)	14, 119, 189, 629, 633, 1377, 2312
MslI	CAYNNNNRTG	4 cut(s)	199, 1470, 1700, 2205
Msp20I	TGGCCA	1 cut(s)	1540
MspA1I	CMGCKG	1 cut(s)	1026
Mva1269I	GAATGC	1 cut(s)	25
MvaI	CCWGG	9 cut(s)	373, 521, 554, 698, 1019, 1067, 1556, 2374, 2452
MvnI	CGCG	1 cut(s)	2177
MwoI	GCNNNNNNNGC	9 cut(s)	601, 843, 905, 941, 1163, 1587, 1727, 1934, 2078
NaeI	GCCGGC	1 cut(s)	844
NcoI	CCATGG	2 cut(s)	1649, 2459
NdeI	CATATG	1 cut(s)	2244
NgoMIV	GCCGGC	1 cut(s)	842
NmeAIII	GCCGAG	1 cut(s)	1887
NmuCI	GTSAC	3 cut(s)	1426, 1442, 1849
NsbI	TGCGCA	1 cut(s)	173
NsiI	ATGCAT	1 cut(s)	2250
NspI	RCATGY	2 cut(s)	106, 1057
OliI	CACNNNNGTG	3 cut(s)	199, 1470, 1700
PacI	TTAATTAA	1 cut(s)	633
PaeR7I	CTCGAG	1 cut(s)	1214
PagI	TCATGA	1 cut(s)	2331
PaqCI	CACCTGC	1 cut(s)	14
PceI	AGGCCT	1 cut(s)	1070
PciI	ACATGT	1 cut(s)	1053
PcsI	WCGNNNNNNNCGW	2 cut(s)	1212, 2169
PctI	GAATGC	1 cut(s)	25
PdiI	GCCGGC	1 cut(s)	844
PfeI	GAWTC	2 cut(s)	363, 998
PflFI	GACNNNGTC	1 cut(s)	2327
PflMI	CCANNNNNTGG	1 cut(s)	1072
PfoI	TCCNGGA	4 cut(s)	1836, 2040, 2372, 2427
PkrI	GCNGC	6 cut(s)	186, 352, 703, 706, 1350, 2032
PleI	GAGTC	4 cut(s)	36, 419, 1841, 2160
PpsI	GAGTC	4 cut(s)	36, 419, 1841, 2160
Ppu21I	YACGTR	2 cut(s)	94, 808
PpuMI	RGGWCCY	2 cut(s)	1220, 2408
PscI	ACATGT	1 cut(s)	1053
PshBI	ATTAAT	2 cut(s)	629, 1377
Psp5II	RGGWCCY	2 cut(s)	1220, 2408
Psp6I	CCWGG	9 cut(s)	371, 519, 552, 696, 1017, 1065, 1554, 2372, 2450
PspEI	GGTNACC	1 cut(s)	1481
PspFI	CCCAGC	2 cut(s)	649, 2469
PspGI	CCWGG	9 cut(s)	371, 519, 552, 696, 1017, 1065, 1554, 2372, 2450
PspPPI	RGGWCCY	2 cut(s)	1220, 2408
PspXI	VCTCGAGB	1 cut(s)	1214
PstI	CTGCAG	3 cut(s)	954, 1350, 1826
PstNI	CAGNNNCTG	1 cut(s)	2476
PsuI	RGATCY	4 cut(s)	775, 1282, 1917, 2365
PsyI	GACNNNGTC	1 cut(s)	2327
RseI	CAYNNNNRTG	4 cut(s)	199, 1470, 1700, 2205
SaqAI	TTAA	7 cut(s)	14, 119, 189, 629, 633, 1377, 2312
SatI	GCNGC	6 cut(s)	185, 351, 702, 705, 1349, 2031
ScaI	AGTACT	1 cut(s)	2127
SchI	GAGTC	4 cut(s)	36, 419, 1842, 2161
SduI	GDGCHC	2 cut(s)	1498, 1618
SexAI	ACCWGGT	1 cut(s)	1017
SfaNI	GCATC	8 cut(s)	171, 213, 855, 917, 1175, 2197, 2278, 2464
SfcI	CTRYAG	4 cut(s)	950, 1346, 1390, 1822
Sfr274I	CTCGAG	1 cut(s)	1214
SinI	GGWCC	5 cut(s)	325, 973, 1194, 1220, 2408
SlaI	CTCGAG	1 cut(s)	1214
SmaI	CCCGGG	3 cut(s)	962, 1079, 2357
SmiMI	CAYNNNNRTG	4 cut(s)	199, 1470, 1700, 2205
SmlI	CTYRAG	2 cut(s)	1214, 2219
SmoI	CTYRAG	2 cut(s)	1214, 2219
SrfI	GCCCGGGC	1 cut(s)	1079
Sse9I	AATT	9 cut(s)	258, 291, 404, 630, 656, 855, 893, 1287, 2315
SseBI	AGGCCT	1 cut(s)	1070
SspI	AATATT	1 cut(s)	1251
SspMI	CTAG	5 cut(s)	767, 834, 968, 1224, 1313
StuI	AGGCCT	1 cut(s)	1070
StyI	CCWWGG	5 cut(s)	1071, 1649, 2016, 2292, 2459
TaaI	ACNGT	6 cut(s)	571, 1240, 1710, 1869, 1955, 2301
TaiI	ACGT	2 cut(s)	96, 810
TasI	AATT	9 cut(s)	258, 291, 404, 630, 656, 855, 893, 1287, 2315
TatI	WGTACW	2 cut(s)	447, 2125
TauI	GCSGC	3 cut(s)	187, 353, 2033
TfiI	GAWTC	2 cut(s)	363, 998
Tru1I	TTAA	7 cut(s)	14, 119, 189, 629, 633, 1377, 2312
Tru9I	TTAA	7 cut(s)	14, 119, 189, 629, 633, 1377, 2312
TscAI	CASTG	4 cut(s)	475, 516, 1670, 1707
TseFI	GTSAC	3 cut(s)	1426, 1442, 1849
TseI	GCWGC	3 cut(s)	701, 704, 1348
Tsp45I	GTSAC	3 cut(s)	1426, 1442, 1849
TspDTI	ATGAA	5 cut(s)	355, 1263, 2348, 2413, 2450
TspGWI	ACGGA	4 cut(s)	395, 1472, 1799, 1868
TspMI	CCCGGG	3 cut(s)	960, 1077, 2355
TspRI	CASTG	4 cut(s)	475, 516, 1670, 1707
Tth111I	GACNNNGTC	1 cut(s)	2327
Van91I	CCANNNNNTGG	1 cut(s)	1072
VpaK11BI	GGWCC	5 cut(s)	325, 973, 1194, 1220, 2408
VspI	ATTAAT	2 cut(s)	629, 1377
XapI	RAATTY	2 cut(s)	656, 893
XceI	RCATGY	2 cut(s)	106, 1057
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1552
XhoI	CTCGAG	1 cut(s)	1214
XmaI	CCCGGG	3 cut(s)	960, 1077, 2355
XmiI	GTMKAC	4 cut(s)	90, 876, 1734, 2343
XspI	CTAG	5 cut(s)	767, 834, 968, 1224, 1313
ZrmI	AGTACT	1 cut(s)	2127
Zsp2I	ATGCAT	1 cut(s)	2250

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.