Rorug05G0324600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000164
Physical Location & Seq
Reverse (-)
38951079 .. 38954621
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0324600.1

Sequence Viewer

Length: 2583 bp
ATGACCCTATTTGGAAAAGTTCAAAGTAGAAGAATTGCCCGGAAGATCAACATTATGCAACTCATCTCATTTATCACCAGCTCAAGTATGAAAATGAGAAAAAAGATGCCATTGGTTCATTCCTACAGAATTGAAAATGCCGAGAAAATGGTTTTGAAAGCTCTGCTTCTCTTTCTCTTCTTTCAGTTCTGCACTTCCAGGGACACCATGAGAGGGAACGAAGGACAATTGAAGGATGCTGATGGTGACTATTTAGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCAGACTAGAGTACCTAATAAAACAGTGGTGTGGATTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATGAACAGGTATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCAGCCCTATATGGTCCACAAATGCGTCACGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTGGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATTATCCTATAGATACTTTAATTCCAGGTATGAAAATTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTATAGGCTAAGTTCAGATCATAATGCATCTCCCCAATTTTTTTTGTATAAAGGTTTGAGTAAGTATTGGCGAAGTGATCCAGGGCCAGCGCCTACTTTGGTCGCTAATCAAGATGAAACTTATTATTTCATGAATGAAACCAATGCAATTGCAAGAATAACAGTGACTGATTCTGCCTTAGAGCGCCTTACATGGGATGGTGGTGGCCTTCAATGGAAGGAAGATTACTCTGCACCGAAGTCCCGTTGTGACAGGTACGGACAGTGTGGTGCCAACAGCAGATGTAGCCCTGATAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGTTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTATAAAGGTGACAAGAGTTAAATATCCAGACACATCGATAGCAGCATCATTAAAATCAGGTATGAGTGGCAAAGAGTGTGCACAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCGATGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGATTGGAAGAGATCTCTATGTTCGTGTGAATGCAACTGTCTTAGCGGCAAATGCCGGAAGATCACAAGGTTTGTGGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTGCAGTGCGCTAGCATTGGTACTAATCATTATGCTTGCATGGAAGTGGCGTAAGAAGAACAGGAACACAAGAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAACAGCAGCCACAGACCACTTCTCTCATGTCAATGAACTTGGCCGCGGTGGCTTTGGCTCTGTTTATATGGGTCAGCTATCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATTGAAGAATTTAAGAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAGGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAGGCTTGGACTCCTTTCTTTTTGATAAGACAAGACGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGTAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGAGACCAACTGCAAGATAAGACAAGCCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCAGTATTTGGGAGATTTTCCACAAAGTCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCAGAAAAACAATGGTTCTGATCCGGAGGATCCTTCCGTGAACTTAATAGGACGTGTATGGGAGCTCTGGAGAGAAGGCACAGCCTTGGATATTGTGGATTCAACACTGAAGTCATATCATCCAAATGAAGCCATGAGATGCATACAAGTTGCTCTCTTGTGTGTACAAGAAGATCCGATGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGATGAAGCATCTCCTCCATTGCCAAAGCAGCCGGCAGTTGTTTACAGAAGAAATTTCGGCACTGATGTTAATCCATTACTTTCAAACAGATCTTTTTCTAGAAATGACTTGACAGTAACTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

860

Amino Acids

97.39

Weight (kDa)

6.44

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 120 - 230 3.9e-35 D-mannose binding lectin
S_locus_glycop PF00954 279 - 355 1.1e-12 S-locus glycoprotein domain
PAN_2 PF08276 379 - 445 1.4e-16 PAN-like domain
Pkinase PF00069 545 - 810 4.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 547 - 812 5.4e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000164)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02320 FvH4_1g21472 FvH4_2g00813 FvH4_2g02512 FvH4_2g03921 FvH4_2g23460 FvH4_3g29141 FvH4_4g05791 FvH4_4g06321 FvH4_4g15595 FvH4_4g18162 FvH4_4g18163 FvH4_4g29541 FvH4_4g29541 FvH4_4g29541 FvH4_4g29541 FvH4_4g29541 FvH4_5g31093 FvH4_6g01982 FvH4_6g21931 FvH4_6g44691 FvH4_6g48541
prunus_persica Prupe.1G074100_v2.0.a1 Prupe.3G021400_v2.0.a1
pyrus_communis pycom02g18570 pycom14g00670
rosa_chinensis RchiOBHm_Chr1g0379201 RchiOBHm_Chr1g0379361 RchiOBHm_Chr4g0405981 RchiOBHm_Chr5g0073641 RchiOBHm_Chr5g0073651 RchiOBHm_Chr7g0236661
rosa_multiflora Rmu_co8179562.1_g000001 Rmu_co8343647.1_g000001 Rmu_sc0000041.1_g000017 Rmu_sc0000114.1_g000020 Rmu_sc0000286.1_g000006 Rmu_sc0000308.1_g000038 Rmu_sc0000365.1_g000061 Rmu_sc0000365.1_g000062 Rmu_sc0000538.1_g000060 Rmu_sc0000538.1_g000061 Rmu_sc0000586.1_g000016 Rmu_sc0000898.1_g000007 Rmu_sc0000913.1_g000003 Rmu_sc0000913.1_g000004 Rmu_sc0000996.1_g000006 Rmu_sc0000996.1_g000007 Rmu_sc0000998.1_g000003 Rmu_sc0001350.1_g000009 Rmu_sc0002079.1_g000009 Rmu_sc0002200.1_g000013 Rmu_sc0002226.1_g000025 Rmu_sc0002279.1_g000014 Rmu_sc0002478.1_g000014 Rmu_sc0002717.1_g000004 Rmu_sc0002868.1_g000027 Rmu_sc0002889.1_g000043 Rmu_sc0003023.1_g000001 Rmu_sc0003110.1_g000006 Rmu_sc0003226.1_g000028 Rmu_sc0003226.1_g000029 Rmu_sc0003337.1_g000053 Rmu_sc0003558.1_g000007 Rmu_sc0004345.1_g000014 Rmu_sc0004383.1_g000007 Rmu_sc0004888.1_g000021 Rmu_sc0005114.1_g000012 Rmu_sc0005223.1_g000019 Rmu_sc0005223.1_g000020 Rmu_sc0005639.1_g000025 Rmu_sc0005685.1_g000027 Rmu_sc0005992.1_g000002 Rmu_sc0006326.1_g000034 Rmu_sc0006326.1_g000035 Rmu_sc0007390.1_g000012 Rmu_sc0007451.1_g000006 Rmu_sc0007825.1_g000003 Rmu_sc0008600.1_g000023 Rmu_sc0009291.1_g000003 Rmu_sc0012046.1_g000001 Rmu_sc0012046.1_g000002 Rmu_sc0012710.1_g000001 Rmu_sc0015238.1_g000001 Rmu_sc0016125.1_g000003 Rmu_sc0016175.1_g000001 Rmu_sc0016898.1_g000001 Rmu_sc0017323.1_g000010 Rmu_sc0017874.1_g000002 Rmu_sc0019167.1_g000001 Rmu_sc0020815.1_g000006 Rmu_sc0025564.1_g000001 Rmu_sc0026418.1_g000001 Rmu_sc0035188.1_g000002 Rmu_sc0039254.1_g000001 Rmu_ssc0000120.1_g000015 Rmu_ssc0000120.1_g000016 Rmu_ssc0000330.1_g000023 Rmu_ssc0000433.1_g000004
rosa_roxburghii Rroxscaffold_1G00047130 Rroxscaffold_1G00051320 Rroxscaffold_1G00056600 Rroxscaffold_2G00109650 Rroxscaffold_2G00119980 Rroxscaffold_3G00222550 Rroxscaffold_3G00224980 Rroxscaffold_3G00251020 Rroxscaffold_6G00411190 Rroxscaffold_7G00166520 Rroxscaffold_7G00172920 Rroxscaffold_7G00191860 Rroxscaffold_7G00194430
rosa_rugosa Rorug01G0198900 Rorug01G0217300 Rorug01G0244500 Rorug02G0147400 Rorug02G0152200 Rorug02G0595500 Rorug02G0595600 Rorug03G0060500 Rorug03G0203000 Rorug03G0285800 Rorug04G0027200 Rorug04G0027300 Rorug04G0225800 Rorug05G0073400 Rorug05G0213300 Rorug05G0324600 Rorug05G0390900 Rorug05G0391000 Rorug05G0391100 Rorug05G0391200 Rorug05G0462900 Rorug05G0473000 Rorug06G0256500 Rorug07G0199000 Rorug07G0208800 Rorug07G0208800 Rorug07G0316600 Rorug07G0316700
rosa_samantha Rh2BG532400 Rh2CG581900 Rh3CG322600 Rh6AG079900 Rh6AG250800 Rh6AG496300 Rh6BG506100 Rh6CG126000 Rh6CG511700 Rh6DG109400 Rh6DG112700 Rh7CG104500 Rh7CG322000 Rh7CG431500
rosa_wichuraiana Rw0G020410 Rw1G009190 Rw1G014580 Rw1G021000 Rw1G022190 Rw2G025900 Rw2G029920 Rw2G037530 Rw3G018560 Rw3G020560 Rw4G008500 Rw4G022520 Rw5G019030 Rw5G031180 Rw5G044830 Rw6G005010 Rw6G006890 Rw6G011330 Rw6G011380 Rw6G023900 Rw7G028490 Rw7G030170 Rw7G030370 Rw7G032030 Rw7G032040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 453, 1157
AasI GACNNNNNNGTC 1 cut(s) 1903
Acc36I ACCTGC 1 cut(s) 2422
AccB1I GGYRCC 1 cut(s) 992
AccII CGCG 1 cut(s) 1656
AccIII TCCGGA 1 cut(s) 2248
AciI CCGC 3 cut(s) 1394, 1654, 1656
AclWI GGATC 6 cut(s) 794, 2207, 2240, 2249, 2262, 2393
AcoI YGGCCR 2 cut(s) 1651, 2224
AcsI RAATTY 5 cut(s) 301, 550, 1751, 2052, 2497
AcuI CTGAAG 3 cut(s) 274, 1716, 2354
AfaI GTAC 6 cut(s) 348, 980, 1343, 1479, 2150, 2391
AfiI CCNNNNNNNGG 4 cut(s) 213, 710, 916, 1868
AflIII ACRYGT 2 cut(s) 2136, 2278
AgeI ACCGGT 1 cut(s) 312
AhlI ACTAGT 2 cut(s) 436, 2015
AjiI CACGTC 1 cut(s) 2279
AjnI CCWGG 6 cut(s) 197, 564, 628, 703, 802, 1048
AleI CACNNNNGTG 1 cut(s) 1240
AluBI AGCT 8 cut(s) 81, 161, 309, 535, 1558, 1687, 2290, 2576
AluI AGCT 8 cut(s) 81, 161, 309, 535, 1558, 1687, 2290, 2576
Alw21I GWGCWC 3 cut(s) 414, 1237, 2292
Alw26I GTCTC 3 cut(s) 1140, 1567, 2082
Alw44I GTGCAC 1 cut(s) 1233
AlwI GGATC 6 cut(s) 794, 2207, 2240, 2249, 2262, 2393
AlwNI CAGNNNCTG 1 cut(s) 890
Aor13HI TCCGGA 1 cut(s) 2248
AoxI GGCC 4 cut(s) 806, 928, 1651, 2224
ApaLI GTGCAC 1 cut(s) 1233
ApeKI GCWGC 4 cut(s) 465, 1196, 1616, 2473
ApoI RAATTY 5 cut(s) 301, 550, 1751, 2052, 2497
AsiGI ACCGGT 1 cut(s) 312
Asp700I GAANNNNTTC 1 cut(s) 1255
AspLEI GCGC 3 cut(s) 814, 909, 1468
AspS9I GGNCC 4 cut(s) 477, 806, 1905, 2407
AsuC2I CCSGG 2 cut(s) 40, 297
AsuHPI GGTGA 6 cut(s) 67, 257, 658, 1174, 1964, 2133
AsuNHI GCTAGC 1 cut(s) 1468
AvaII GGWCC 3 cut(s) 477, 1905, 2407
BaeGI GKGCMC 1 cut(s) 1237
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BalI TGGCCA 1 cut(s) 2226
BamHI GGATCC 1 cut(s) 2254
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 2292
BarI GAAGNNNNNNTAC 2 cut(s) 932, 964
BauI CACGAG 1 cut(s) 1953
Bbv12I GWGCWC 3 cut(s) 414, 1237, 2292
BbvI GCAGC 4 cut(s) 477, 1208, 1628, 2485
BccI CCATC 6 cut(s) 236, 398, 914, 1136, 1321, 2398
BciT130I CCWGG 6 cut(s) 199, 566, 630, 705, 804, 1050
BciVI GTATCC 1 cut(s) 535
BcnI CCSGG 2 cut(s) 40, 297
BcoDI GTCTC 3 cut(s) 1140, 1567, 2082
BcuI ACTAGT 2 cut(s) 436, 2015
BfaI CTAG 9 cut(s) 306, 342, 437, 1469, 1850, 2016, 2030, 2073, 2544
BfmI CTRYAG 3 cut(s) 124, 612, 724
BfoI RGCGCY 2 cut(s) 815, 910
BfuAI ACCTGC 1 cut(s) 2422
BfuI GTATCC 1 cut(s) 535
BglI GCCNNNNNGGC 1 cut(s) 1659
BglII AGATCT 3 cut(s) 1360, 2005, 2534
BisI GCNGC 6 cut(s) 466, 1197, 1395, 1617, 1654, 2474
BlsI GCNGC 6 cut(s) 467, 1198, 1396, 1618, 1655, 2475
Bme1390I CCNGG 8 cut(s) 40, 199, 297, 566, 630, 705, 804, 1050
Bme18I GGWCC 3 cut(s) 477, 1905, 2407
BmgBI CACGTC 1 cut(s) 2279
BmgT120I GGNCC 4 cut(s) 477, 806, 1905, 2407
BmiI GGNNCC 3 cut(s) 994, 1796, 2256
BmrFI CCNGG 8 cut(s) 40, 199, 297, 566, 630, 705, 804, 1050
BmrI ACTGGG 2 cut(s) 669, 720
BmsI GCATC 6 cut(s) 96, 226, 758, 1208, 2354, 2462
BmtI GCTAGC 1 cut(s) 1472
BmuI ACTGGG 2 cut(s) 669, 720
BpmI CTGGAG 1 cut(s) 2314
BpuEI CTTGAG 3 cut(s) 67, 1580, 1966
BpuMI CCSGG 2 cut(s) 40, 297
Bsa29I ATCGAT 1 cut(s) 1190
BsaBI GATNNNNATC 2 cut(s) 1080, 2514
BsaI GGTCTC 1 cut(s) 2082
BsaJI CCNNGG 8 cut(s) 198, 295, 703, 803, 1049, 1654, 2083, 2310
BsaWI WCCGGW 3 cut(s) 312, 2143, 2248
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 4 cut(s) 213, 710, 916, 1868
Bse118I RCCGGY 2 cut(s) 312, 2476
Bse1I ACTGG 2 cut(s) 664, 715
Bse3DI GCAATG 1 cut(s) 2462
Bse8I GATNNNNATC 2 cut(s) 1080, 2514
BseAI TCCGGA 1 cut(s) 2248
BseBI CCWGG 6 cut(s) 199, 566, 630, 705, 804, 1050
BseCI ATCGAT 1 cut(s) 1190
BseDI CCNNGG 8 cut(s) 198, 295, 703, 803, 1049, 1654, 2083, 2310
BseGI GGATG 6 cut(s) 241, 574, 925, 1109, 1845, 2344
BseJI GATNNNNATC 2 cut(s) 1080, 2514
BseLI CCNNNNNNNGG 4 cut(s) 213, 710, 916, 1868
BseMI GCAATG 1 cut(s) 2462
BseMII CTCAG 2 cut(s) 350, 1583
BseNI ACTGG 2 cut(s) 664, 715
BseRI GAGGAG 2 cut(s) 1568, 2448
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 4 cut(s) 477, 1208, 1628, 2485
BsgI GTGCAG 4 cut(s) 175, 513, 939, 1480
Bsh1236I CGCG 1 cut(s) 1656
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 4 cut(s) 808, 930, 1653, 2226
BshNI GGYRCC 1 cut(s) 992
BshTI ACCGGT 1 cut(s) 312
BshVI ATCGAT 1 cut(s) 1190
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 3 cut(s) 414, 1237, 2292
BsiSI CCGG 7 cut(s) 40, 297, 313, 1404, 2144, 2249, 2477
BslFI GGGAC 3 cut(s) 215, 728, 949
BslI CCNNNNNNNGG 4 cut(s) 213, 710, 916, 1868
BsmAI GTCTC 3 cut(s) 1140, 1567, 2082
BsmBI CGTCTC 1 cut(s) 1140
BsmFI GGGAC 3 cut(s) 215, 728, 949
BsmI GAATGC 1 cut(s) 1384
BsnI GGCC 4 cut(s) 808, 930, 1653, 2226
Bso31I GGTCTC 1 cut(s) 2082
Bsp1286I GDGCHC 3 cut(s) 414, 1237, 2292
Bsp13I TCCGGA 1 cut(s) 2248
Bsp1407I TGTACA 2 cut(s) 1341, 2389
Bsp19I CCATGG 1 cut(s) 2083
BspACI CCGC 3 cut(s) 1394, 1654, 1656
BspANI GGCC 4 cut(s) 808, 930, 1653, 2226
BspCNI CTCAG 2 cut(s) 349, 1582
BspDI ATCGAT 1 cut(s) 1190
BspEI TCCGGA 1 cut(s) 2248
BspFNI CGCG 1 cut(s) 1656
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 3 cut(s) 994, 1796, 2256
BspMI ACCTGC 1 cut(s) 2422
BspOI GCTAGC 1 cut(s) 1472
BspPI GGATC 6 cut(s) 794, 2207, 2240, 2249, 2262, 2393
BspT107I GGYRCC 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 2082
BsrDI GCAATG 1 cut(s) 2462
BsrFI RCCGGY 2 cut(s) 312, 2476
BsrGI TGTACA 2 cut(s) 1341, 2389
BsrI ACTGG 2 cut(s) 664, 715
BssAI RCCGGY 2 cut(s) 312, 2476
BssECI CCNNGG 8 cut(s) 198, 295, 703, 803, 1049, 1654, 2083, 2310
BssSI CACGAG 1 cut(s) 1953
BssT1I CCWWGG 2 cut(s) 2083, 2310
Bst2BI CACGAG 1 cut(s) 1953
Bst2UI CCWGG 6 cut(s) 199, 566, 630, 705, 804, 1050
Bst4CI ACNGT 9 cut(s) 361, 495, 886, 987, 1043, 1387, 1458, 1905, 2560
Bst6I CTCTTC 2 cut(s) 182, 1351
BstAUI TGTACA 2 cut(s) 1341, 2389
BstC8I GCNNGC 5 cut(s) 810, 1443, 1470, 1494, 2478
BstDEI CTNAG 6 cut(s) 336, 731, 901, 1247, 1390, 1569
BstDSI CCRYGG 2 cut(s) 1654, 2083
BstENI CCTNNNNNAGG 1 cut(s) 1866
BstF5I GGATG 6 cut(s) 241, 574, 925, 1109, 1845, 2344
BstFNI CGCG 1 cut(s) 1656
BstH2I RGCGCY 2 cut(s) 815, 910
BstHHI GCGC 3 cut(s) 814, 909, 1468
BstMAI GTCTC 3 cut(s) 1140, 1567, 2082
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 6 cut(s) 1008, 1400, 1659, 1775, 2423, 2473
BstNI CCWGG 6 cut(s) 199, 566, 630, 705, 804, 1050
BstNSI RCATGY 2 cut(s) 1861, 2140
BstSCI CCNGG 8 cut(s) 38, 197, 295, 564, 628, 703, 802, 1048
BstSFI CTRYAG 3 cut(s) 124, 612, 724
BstSLI GKGCMC 1 cut(s) 1237
BstUI CGCG 1 cut(s) 1656
BstV1I GCAGC 4 cut(s) 477, 1208, 1628, 2485
BstX2I RGATCY 5 cut(s) 1360, 2005, 2254, 2398, 2534
BstYI RGATCY 5 cut(s) 1360, 2005, 2254, 2398, 2534
Bsu15I ATCGAT 1 cut(s) 1190
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 4 cut(s) 808, 930, 1653, 2226
BsuTUI ATCGAT 1 cut(s) 1190
BtgI CCRYGG 2 cut(s) 1654, 2083
BtgZI GCGATG 1 cut(s) 1293
BtrI CACGTC 1 cut(s) 2279
BtsCI GGATG 6 cut(s) 241, 574, 925, 1109, 1845, 2344
BtsI GCAGTG 2 cut(s) 1263, 1468
BtsIMutI CAGTG 7 cut(s) 366, 891, 992, 1263, 1468, 2330, 2505
BveI ACCTGC 1 cut(s) 2422
Cac8I GCNNGC 5 cut(s) 810, 1443, 1470, 1494, 2478
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 1 cut(s) 852
CfoI GCGC 3 cut(s) 814, 909, 1468
Cfr10I RCCGGY 2 cut(s) 312, 2476
Cfr13I GGNCC 4 cut(s) 477, 806, 1905, 2407
Cfr42I CCGCGG 1 cut(s) 1657
ClaI ATCGAT 1 cut(s) 1190
CseI GACGC 1 cut(s) 477
Csp6I GTAC 6 cut(s) 347, 979, 1342, 1478, 2149, 2390
CspAI ACCGGT 1 cut(s) 312
CspCI CAANNNNNGTGG 2 cut(s) 1403, 1438
CviQI GTAC 6 cut(s) 347, 979, 1342, 1478, 2149, 2390
DdeI CTNAG 6 cut(s) 336, 731, 901, 1247, 1390, 1569
DrdI GACNNNNNNGTC 1 cut(s) 1903
DseDI GACNNNNNNGTC 1 cut(s) 1903
EaeI YGGCCR 2 cut(s) 1651, 2224
Eam1104I CTCTTC 2 cut(s) 182, 1351
EarI CTCTTC 2 cut(s) 182, 1351
Ecl136II GAGCTC 1 cut(s) 2290
Eco130I CCWWGG 2 cut(s) 2083, 2310
Eco24I GRGCYC 1 cut(s) 2292
Eco31I GGTCTC 1 cut(s) 2082
Eco47I GGWCC 3 cut(s) 477, 1905, 2407
Eco53kI GAGCTC 1 cut(s) 2290
Eco57I CTGAAG 3 cut(s) 274, 1716, 2354
EcoICRI GAGCTC 1 cut(s) 2290
EcoNI CCTNNNNNAGG 1 cut(s) 1866
EcoRII CCWGG 6 cut(s) 197, 564, 628, 703, 802, 1048
EcoT14I CCWWGG 2 cut(s) 2083, 2310
EcoT22I ATGCAT 2 cut(s) 751, 2369
EcoT38I GRGCYC 1 cut(s) 2292
ErhI CCWWGG 2 cut(s) 2083, 2310
Esp3I CGTCTC 1 cut(s) 1140
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 1755, 1787
FaqI GGGAC 3 cut(s) 215, 728, 949
FauNDI CATATG 1 cut(s) 2128
Fnu4HI GCNGC 6 cut(s) 466, 1197, 1395, 1617, 1654, 2474
FokI GGATG 6 cut(s) 248, 581, 932, 1116, 1852, 2331
FriOI GRGCYC 1 cut(s) 2292
Fsp4HI GCNGC 6 cut(s) 466, 1197, 1395, 1617, 1654, 2474
FspBI CTAG 9 cut(s) 306, 342, 437, 1469, 1850, 2016, 2030, 2073, 2544
GlaI GCGC 3 cut(s) 813, 908, 1467
GluI GCNGC 6 cut(s) 466, 1197, 1395, 1617, 1654, 2474
GsuI CTGGAG 1 cut(s) 2314
HaeII RGCGCY 2 cut(s) 815, 910
HaeIII GGCC 4 cut(s) 808, 930, 1653, 2226
HapII CCGG 7 cut(s) 40, 297, 313, 1404, 2144, 2249, 2477
HgaI GACGC 1 cut(s) 477
HhaI GCGC 3 cut(s) 814, 909, 1468
Hin6I GCGC 3 cut(s) 812, 907, 1466
HinP1I GCGC 3 cut(s) 812, 907, 1466
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 7 cut(s) 324, 893, 1081, 1877, 1960, 1979, 2324
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 7 cut(s) 40, 297, 313, 1404, 2144, 2249, 2477
HphI GGTGA 6 cut(s) 67, 257, 658, 1174, 1964, 2133
Hpy166II GTNNAC 8 cut(s) 480, 511, 1235, 1342, 1344, 2266, 2390, 2488
Hpy188I TCNGA 9 cut(s) 339, 739, 1086, 1572, 2059, 2123, 2182, 2245, 2403
Hpy188III TCNNGA 9 cut(s) 833, 853, 1181, 1601, 1734, 1983, 2249, 2293, 2544
Hpy8I GTNNAC 8 cut(s) 480, 511, 1235, 1342, 1344, 2266, 2390, 2488
HpyAV CCTTC 8 cut(s) 215, 226, 934, 941, 1283, 1918, 2268, 2294
HpyCH4III ACNGT 9 cut(s) 361, 495, 886, 987, 1043, 1387, 1458, 1905, 2560
HpyCH4IV ACGT 1 cut(s) 2278
HpyF10VI GCNNNNNNNGC 6 cut(s) 1008, 1400, 1659, 1775, 2423, 2473
HpyF3I CTNAG 6 cut(s) 336, 731, 901, 1247, 1390, 1569
HpySE526I ACGT 1 cut(s) 2278
HspAI GCGC 3 cut(s) 812, 907, 1466
Kpn2I TCCGGA 1 cut(s) 2248
KroI GCCGGC 1 cut(s) 2476
KroNI GCCGGC 1 cut(s) 2478
KspAI GTTAAC 1 cut(s) 511
KspI CCGCGG 1 cut(s) 1657
LmnI GCTCC 2 cut(s) 1555, 2287
Lsp1109I GCAGC 4 cut(s) 477, 1208, 1628, 2485
LweI GCATC 6 cut(s) 96, 226, 758, 1208, 2354, 2462
MaeI CTAG 9 cut(s) 306, 342, 437, 1469, 1850, 2016, 2030, 2073, 2544
MaeII ACGT 1 cut(s) 2278
MaeIII GTNAC 9 cut(s) 245, 489, 690, 886, 971, 1037, 1162, 2139, 2560
MfeI CAATTG 2 cut(s) 227, 870
MflI RGATCY 5 cut(s) 1360, 2005, 2254, 2398, 2534
MhlI GDGCHC 3 cut(s) 414, 1237, 2292
MlsI TGGCCA 1 cut(s) 2226
MluNI TGGCCA 1 cut(s) 2226
MlyI GAGTC 2 cut(s) 1871, 1973
MmeI TCCRAC 3 cut(s) 301, 2101, 2190
Mox20I TGGCCA 1 cut(s) 2226
Mph1103I ATGCAT 2 cut(s) 751, 2369
MroI TCCGGA 1 cut(s) 2248
MroNI GCCGGC 1 cut(s) 2476
MroXI GAANNNNTTC 1 cut(s) 1255
MscI TGGCCA 1 cut(s) 2226
MseI TTAA 9 cut(s) 510, 623, 677, 1023, 1173, 1205, 1755, 2270, 2514
MslI CAYNNNNRTG 5 cut(s) 1240, 1337, 1501, 1641, 2349
Msp20I TGGCCA 1 cut(s) 2226
MspA1I CMGCKG 1 cut(s) 1656
MspI CCGG 7 cut(s) 40, 297, 313, 1404, 2144, 2249, 2477
MspR9I CCNGG 8 cut(s) 40, 199, 297, 566, 630, 705, 804, 1050
MunI CAATTG 2 cut(s) 227, 870
Mva1269I GAATGC 1 cut(s) 1384
MvaI CCWGG 6 cut(s) 199, 566, 630, 705, 804, 1050
MvnI CGCG 1 cut(s) 1656
MwoI GCNNNNNNNGC 6 cut(s) 1008, 1400, 1659, 1775, 2423, 2473
NaeI GCCGGC 1 cut(s) 2478
NciI CCSGG 2 cut(s) 40, 297
NcoI CCATGG 1 cut(s) 2083
NdeI CATATG 1 cut(s) 2128
NgoMIV GCCGGC 1 cut(s) 2476
NheI GCTAGC 1 cut(s) 1468
NlaIV GGNNCC 3 cut(s) 994, 1796, 2256
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 8 cut(s) 245, 489, 690, 886, 971, 1037, 1162, 2139
NsiI ATGCAT 2 cut(s) 751, 2369
NspI RCATGY 2 cut(s) 1861, 2140
OliI CACNNNNGTG 1 cut(s) 1240
PagI TCATGA 1 cut(s) 852
PciI ACATGT 1 cut(s) 2136
PctI GAATGC 1 cut(s) 1384
PdiI GCCGGC 1 cut(s) 2478
PdmI GAANNNNTTC 1 cut(s) 1255
PfeI GAWTC 5 cut(s) 324, 893, 1081, 1960, 2324
PfoI TCCNGGA 1 cut(s) 564
PinAI ACCGGT 1 cut(s) 312
PkrI GCNGC 6 cut(s) 467, 1198, 1396, 1618, 1655, 2475
PleI GAGTC 2 cut(s) 1871, 1973
PpsI GAGTC 2 cut(s) 1871, 1973
PscI ACATGT 1 cut(s) 2136
PsiI TTATAA 2 cut(s) 453, 1157
Psp124BI GAGCTC 1 cut(s) 2292
Psp6I CCWGG 6 cut(s) 197, 564, 628, 703, 802, 1048
PspGI CCWGG 6 cut(s) 197, 564, 628, 703, 802, 1048
PspN4I GGNNCC 3 cut(s) 994, 1796, 2256
PspPI GGNCC 4 cut(s) 477, 806, 1905, 2407
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 5 cut(s) 1360, 2005, 2254, 2398, 2534
RsaI GTAC 6 cut(s) 348, 980, 1343, 1479, 2150, 2391
RsaNI GTAC 6 cut(s) 347, 979, 1342, 1478, 2149, 2390
RseI CAYNNNNRTG 5 cut(s) 1240, 1337, 1501, 1641, 2349
SacI GAGCTC 1 cut(s) 2292
SacII CCGCGG 1 cut(s) 1657
SaqAI TTAA 9 cut(s) 510, 623, 677, 1023, 1173, 1205, 1755, 2270, 2514
SatI GCNGC 6 cut(s) 466, 1197, 1395, 1617, 1654, 2474
Sau96I GGNCC 4 cut(s) 477, 806, 1905, 2407
SchI GAGTC 2 cut(s) 1871, 1973
ScrFI CCNGG 8 cut(s) 40, 199, 297, 566, 630, 705, 804, 1050
SduI GDGCHC 3 cut(s) 414, 1237, 2292
SfaNI GCATC 6 cut(s) 96, 226, 758, 1208, 2354, 2462
SfcI CTRYAG 3 cut(s) 124, 612, 724
Sfr303I CCGCGG 1 cut(s) 1657
SgrBI CCGCGG 1 cut(s) 1657
SinI GGWCC 3 cut(s) 477, 1905, 2407
SmiMI CAYNNNNRTG 5 cut(s) 1240, 1337, 1501, 1641, 2349
SmlI CTYRAG 3 cut(s) 82, 1559, 1981
SmoI CTYRAG 3 cut(s) 82, 1559, 1981
SpeI ACTAGT 2 cut(s) 436, 2015
SsiI CCGC 3 cut(s) 1394, 1654, 1656
SspI AATATT 1 cut(s) 2079
SspMI CTAG 9 cut(s) 306, 342, 437, 1469, 1850, 2016, 2030, 2073, 2544
SstI GAGCTC 1 cut(s) 2292
StyD4I CCNGG 8 cut(s) 38, 197, 295, 564, 628, 703, 802, 1048
StyI CCWWGG 2 cut(s) 2083, 2310
TaaI ACNGT 9 cut(s) 361, 495, 886, 987, 1043, 1387, 1458, 1905, 2560
TaiI ACGT 1 cut(s) 2281
TaqI TCGA 4 cut(s) 1117, 1190, 1596, 2578
TaqII GACCGA 1 cut(s) 2424
TatI WGTACW 2 cut(s) 1341, 2389
TauI GCSGC 2 cut(s) 1397, 1656
TfiI GAWTC 5 cut(s) 324, 893, 1081, 1960, 2324
Tru1I TTAA 9 cut(s) 510, 623, 677, 1023, 1173, 1205, 1755, 2270, 2514
Tru9I TTAA 9 cut(s) 510, 623, 677, 1023, 1173, 1205, 1755, 2270, 2514
TscAI CASTG 7 cut(s) 366, 891, 992, 1270, 1468, 2337, 2512
TseFI GTSAC 8 cut(s) 245, 489, 690, 886, 971, 1037, 1162, 2139
TseI GCWGC 4 cut(s) 465, 1196, 1616, 2473
Tsp45I GTSAC 8 cut(s) 245, 489, 690, 886, 971, 1037, 1162, 2139
TspGWI ACGGA 2 cut(s) 996, 2251
TspRI CASTG 7 cut(s) 366, 891, 992, 1270, 1468, 2337, 2512
VneI GTGCAC 1 cut(s) 1233
VpaK11BI GGWCC 3 cut(s) 477, 1905, 2407
XagI CCTNNNNNAGG 1 cut(s) 1866
XapI RAATTY 5 cut(s) 301, 550, 1751, 2052, 2497
XbaI TCTAGA 1 cut(s) 2543
XceI RCATGY 2 cut(s) 1861, 2140
XcmI CCANNNNNNNNNTGG 1 cut(s) 2234
XmnI GAANNNNTTC 1 cut(s) 1255
XspI CTAG 9 cut(s) 306, 342, 437, 1469, 1850, 2016, 2030, 2073, 2544
Zsp2I ATGCAT 2 cut(s) 751, 2369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.