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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 65 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 39 |
| AgsI | TTSAA | 1 cut(s) | 151 |
| ApoI | RAATTY | 1 cut(s) | 65 |
| AspS9I | GGNCC | 1 cut(s) | 30 |
| AvaII | GGWCC | 1 cut(s) | 30 |
| BfaI | CTAG | 1 cut(s) | 141 |
| Bme18I | GGWCC | 1 cut(s) | 30 |
| BmgT120I | GGNCC | 1 cut(s) | 30 |
| BmiI | GGNNCC | 1 cut(s) | 31 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 39 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 39 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 39 |
| BspLI | GGNNCC | 1 cut(s) | 31 |
| Bst4CI | ACNGT | 1 cut(s) | 50 |
| Bst6I | CTCTTC | 1 cut(s) | 60 |
| Cfr13I | GGNCC | 1 cut(s) | 30 |
| CviJI | RGCY | 2 cut(s) | 5, 112 |
| CviKI_1 | RGCY | 2 cut(s) | 5, 112 |
| Eam1104I | CTCTTC | 1 cut(s) | 60 |
| EarI | CTCTTC | 1 cut(s) | 60 |
| Eco47I | GGWCC | 1 cut(s) | 30 |
| FaiI | YATR | 2 cut(s) | 8, 46 |
| FspBI | CTAG | 1 cut(s) | 141 |
| HinfI | GANTC | 2 cut(s) | 80, 137 |
| Hpy188III | TCNNGA | 1 cut(s) | 59 |
| HpyAV | CCTTC | 1 cut(s) | 157 |
| HpyCH4III | ACNGT | 1 cut(s) | 50 |
| LpnPI | CCDG | 1 cut(s) | 3 |
| MaeI | CTAG | 1 cut(s) | 141 |
| MboII | GAAGA | 3 cut(s) | 47, 140, 167 |
| MluCI | AATT | 2 cut(s) | 65, 166 |
| MlyI | GAGTC | 2 cut(s) | 89, 131 |
| MnlI | CCTC | 1 cut(s) | 127 |
| MseI | TTAA | 3 cut(s) | 12, 38, 169 |
| NlaIV | GGNNCC | 1 cut(s) | 31 |
| PleI | GAGTC | 2 cut(s) | 88, 131 |
| PpsI | GAGTC | 2 cut(s) | 88, 131 |
| PspN4I | GGNNCC | 1 cut(s) | 31 |
| PspPI | GGNCC | 1 cut(s) | 30 |
| SaqAI | TTAA | 3 cut(s) | 12, 38, 169 |
| Sau96I | GGNCC | 1 cut(s) | 30 |
| SchI | GAGTC | 2 cut(s) | 89, 131 |
| SetI | ASST | 1 cut(s) | 44 |
| SgeI | CNNG | 6 cut(s) | 30, 71, 88, 96, 121, 153 |
| SinI | GGWCC | 1 cut(s) | 30 |
| Sse9I | AATT | 2 cut(s) | 65, 166 |
| SspMI | CTAG | 1 cut(s) | 141 |
| TaaI | ACNGT | 1 cut(s) | 50 |
| TasI | AATT | 2 cut(s) | 65, 166 |
| Tru1I | TTAA | 3 cut(s) | 12, 38, 169 |
| Tru9I | TTAA | 3 cut(s) | 12, 38, 169 |
| TspDTI | ATGAA | 2 cut(s) | 141, 168 |
| VpaK11BI | GGWCC | 1 cut(s) | 30 |
| XapI | RAATTY | 1 cut(s) | 65 |
| XspI | CTAG | 1 cut(s) | 141 |