FvH4_4g03110

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000188
Physical Location & Seq
Reverse (-)
2782640 .. 2783935
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03110.t1

Sequence Viewer

Length: 1134 bp
ATGGAAGATCATGGCAGTAGAAATATTGATGTTGAAGCATTGGAACGGAGCATGGAAGCAAAGCTTTGCAGGAATTCGCCCTTGTCTGCTAAGTGCTGCATCTTCAGAGTTCCTGAGTTACTCCGGAGACATAAACCAGAGGCATATCAACCTGATGTTGTCTCAATCGGACCCCTCCATCACCATAACGGGGTTGATGATAGTATTACCGGGTTTGAGAAACGTGCCCGTGATTATTATGCAGACTCACTTGATCATCTTAATGGCAATGAATTCGATTCAGATCAGGCTAATGATCCCATAACCAACATGGATTTCATGATCCAGTATCTTTTTCATGATCTTCTGCTACTAGAAAATCAGCTTCCTTGGTTTGTTCTCGAGTCTCTACATAGACTTATCGGCAAAAATCAAGTCCCCCTCACTATGCTCGTGCTCAACTTCTTCAGCAGACTATCATCAATGTCCCGTTATTGCAAGGGTTATTTCAAACACACTCATGATGAGGAGATTCTACACATACTTGATCTGCTAAGAAGTGCTATTGTTGTTCCATTCCAAAAATTTGAATCAACTGAATACGATAATGCACTGCCCCATGCGACTGCTCTCTTGGAGGCAGGCATTGAATTCAGAAGAAGCTCAGTTGATGGCATAATGAATATTGATTTCAAAGATGGAGTTCTCACAATTCCACAGCTAGCAATTGAAGAAATGACTGAACCTTTGTTCAGGAACCTCATAGCCTTCGAGCAATGCCATCATGGTCGCGAGAATAAAGTAACTTCTTATGCACTTATACTGGATAACCTTATTGCTTCCAGCAAGGATGTTGTTTTTCTTTGTGACAAACAAATAATCCACAATTGGTTGAGTGCTGAAGATGTTTCCCAGTTGTTTGGTAAGCTTCATTGTGACACATTCACTGTTGGATTTTGCTATGGCGGGCTTTGCGCTGAAGTGAATAATTATTACCAGGCTACATGGAACAAGTGGCTGGCCGGATTGAAGCGCGATTACTTATCTAATCCGTGGAAAATCACTTCTTTGGTAGTAGCCTTCATCCTTCTGCTGGTTCTCACCCTATTGCAGACAACATATACCATTCAGCAATACTATCATCCTCATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

378

Amino Acids

43.46

Weight (kDa)

5.59

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 34 - 63 3.3e-08 Plant protein of unknown function
DUF247 PF03140 93 - 358 5.4e-73 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000188)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02500 FvH4_4g03110 FvH4_5g11900 FvH4_5g11910 FvH4_5g19510 FvH4_6g53141 FvH4_6g53141 FvH4_6g53143 FvH4_6g53143 FvH4_6g53260 FvH4_6g53260 FvH4_6g53260 FvH4_6g53280
malus_domestica MD00G1110800.v1.1 MD00G1111300.v1.1 MD08G1225300.v1.1 MD08G1225500.v1.1 MD08G1225700.v1.1 MD09G1013500.v1.1 MD09G1013700.v1.1 MD09G1013800.v1.1 MD09G1014000.v1.1
prunus_persica Prupe.1G028300_v2.0.a1 Prupe.1G029300_v2.0.a1 Prupe.1G029400_v2.0.a1 Prupe.1G029800_v2.0.a1 Prupe.1G165900_v2.0.a1 Prupe.3G303500_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029200_v2.0.a1 Prupe.7G037400_v2.0.a1
pyrus_communis pycom08g19660 pycom08g19690 pycom08g19710 pycom08g19720 pycom111g01160 pycom111g01170 pycom17g01140
rosa_chinensis RchiOBHm_Chr2g0111831 RchiOBHm_Chr2g0138211 RchiOBHm_Chr2g0163091 RchiOBHm_Chr2g0175051 RchiOBHm_Chr2g0175141 RchiOBHm_Chr2g0175171 RchiOBHm_Chr2g0175191 RchiOBHm_Chr3g0483151 RchiOBHm_Chr3g0483161 RchiOBHm_Chr3g0483171 RchiOBHm_Chr4g0389941 RchiOBHm_Chr4g0411021 RchiOBHm_Chr4g0411031 RchiOBHm_Chr4g0411041 RchiOBHm_Chr5g0041861 RchiOBHm_Chr5g0058261 RchiOBHm_Chr5g0065661 RchiOBHm_Chr5g0070431 RchiOBHm_Chr7g0185221 RchiOBHm_Chr7g0185581 RchiOBHm_Chr7g0185591 RchiOBHm_Chr7g0185611 RchiOBHm_Chr7g0185641
rosa_laevigata RLG00000004895 RLG00000004896 RLG00000004897 RLG00000008379 RLG00000021370 RLG00000022309 RLG00000034099 RLG00000035811 RLG00000036132 RLG00000036450
rosa_multiflora Rmu_co8229993.1_g000001 Rmu_co8360165.1_g000001 Rmu_co8461695.1_g000001 Rmu_sc0000213.1_g000004 Rmu_sc0000235.1_g000066 Rmu_sc0000367.1_g000060 Rmu_sc0000861.1_g000057 Rmu_sc0000861.1_g000068 Rmu_sc0001244.1_g000011 Rmu_sc0001244.1_g000013 Rmu_sc0001323.1_g000018 Rmu_sc0002180.1_g000032 Rmu_sc0002280.1_g000009 Rmu_sc0003226.1_g000095 Rmu_sc0003226.1_g000096 Rmu_sc0003555.1_g000010 Rmu_sc0003555.1_g000018 Rmu_sc0003920.1_g000011 Rmu_sc0003984.1_g000012 Rmu_sc0004336.1_g000026 Rmu_sc0006027.1_g000005 Rmu_sc0006570.1_g000004 Rmu_sc0007961.1_g000010 Rmu_sc0008747.1_g000001 Rmu_sc0008834.1_g000005 Rmu_sc0010190.1_g000004 Rmu_sc0010190.1_g000007 Rmu_sc0010190.1_g000008 Rmu_sc0010642.1_g000012 Rmu_sc0011497.1_g000002
rosa_roxburghii Rroxscaffold_1G00010770 Rroxscaffold_1G00010780 Rroxscaffold_1G00011860 Rroxscaffold_1G00015470 Rroxscaffold_1G00038670 Rroxscaffold_2G00077520 Rroxscaffold_2G00077550 Rroxscaffold_2G00106420 Rroxscaffold_3G00268750 Rroxscaffold_3G00268770 Rroxscaffold_3G00268780 Rroxscaffold_3G00268790 Rroxscaffold_3G00269130 Rroxscaffold_5G00336020 Rroxscaffold_5G00343150 Rroxscaffold_5G00355700
rosa_rugosa Rorug02G0500300 Rorug02G0581200 Rorug03G0204700 Rorug03G0327300 Rorug05G0196100 Rorug05G0196400 Rorug05G0405900 Rorug05G0406000 Rorug06G0467000 Rorug06G0467000 Rorug06G0470200 Rorug06G0470200 Rorug07G0180500
rosa_samantha Rh2AG566700 Rh2AG661900 Rh2AG663200 Rh3CG286400 Rh3CG286500 Rh3CG366100 Rh4DG024200 Rh4DG076600 Rh4DG160000 Rh4DG160100 Rh5BG286800 Rh5DG295500 Rh5DG461000 Rh7DG072100 Rh7DG075500
rosa_wichuraiana Rw1G002310 Rw1G015820 Rw2G046920 Rw2G054280 Rw3G022880 Rw3G029170 Rw4G002330 Rw4G013670 Rw5G026420 Rw5G026440 Rw5G040490 Rw5G042130 Rw7G006300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 771, 1014
AccIII TCCGGA 1 cut(s) 123
AciI CCGC 1 cut(s) 945
AclWI GGATC 2 cut(s) 290, 316
AcoI YGGCCR 1 cut(s) 999
AcsI RAATTY 4 cut(s) 73, 272, 563, 629
AcuI CTGAAG 4 cut(s) 88, 430, 900, 978
AfiI CCNNNNNNNGG 2 cut(s) 190, 1072
AgsI TTSAA 7 cut(s) 35, 490, 569, 629, 673, 710, 1009
AjnI CCWGG 1 cut(s) 975
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 5 cut(s) 64, 364, 642, 700, 907
AluI AGCT 5 cut(s) 64, 364, 642, 700, 907
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 3 cut(s) 121, 166, 390
AlwI GGATC 2 cut(s) 290, 316
Ama87I CYCGRG 1 cut(s) 380
Aor13HI TCCGGA 1 cut(s) 123
AoxI GGCC 1 cut(s) 999
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 4 cut(s) 73, 272, 563, 629
ArsI GACNNNNNNTTYG 4 cut(s) 399, 431, 709, 741
AspLEI GCGC 2 cut(s) 956, 1014
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 173, 1072
AsuNHI GCTAGC 1 cut(s) 700
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 1001, 1033
BauI CACGAG 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 83
BccI CCATC 4 cut(s) 186, 644, 671, 768
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 977
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 121, 166, 390
BfaI CTAG 2 cut(s) 353, 701
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 2 cut(s) 211, 977
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 172, 737
BmrFI CCNGG 2 cut(s) 211, 977
BmrI ACTGGG 1 cut(s) 886
BmsI GCATC 1 cut(s) 108
BmtI GCTAGC 1 cut(s) 704
BmuI ACTGGG 1 cut(s) 886
BpuMI CCSGG 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 368, 1031
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 1072
Bse1I ACTGG 3 cut(s) 325, 807, 892
Bse3DI GCAATG 2 cut(s) 274, 761
Bse8I GATNNNNATC 1 cut(s) 282
BseAI TCCGGA 1 cut(s) 123
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 2 cut(s) 368, 1031
BseGI GGATG 3 cut(s) 835, 1062, 1120
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 190, 1072
BseMI GCAATG 2 cut(s) 274, 761
BseMII CTCAG 2 cut(s) 105, 657
BseNI ACTGG 3 cut(s) 325, 807, 892
BseRI GAGGAG 1 cut(s) 521
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 1 cut(s) 83
Bsh1236I CGCG 2 cut(s) 771, 1014
BshFI GGCC 1 cut(s) 1001
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 124, 210, 1002
BslFI GGGAC 2 cut(s) 401, 451
BslI CCNNNNNNNGG 2 cut(s) 190, 1072
BsmAI GTCTC 3 cut(s) 121, 166, 390
BsmFI GGGAC 2 cut(s) 401, 451
BsnI GGCC 1 cut(s) 1001
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 229, 438
Bsp13I TCCGGA 1 cut(s) 123
Bsp143I GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
Bsp68I TCGCGA 1 cut(s) 771
BspACI CCGC 1 cut(s) 945
BspANI GGCC 1 cut(s) 1001
BspCNI CTCAG 2 cut(s) 106, 656
BspEI TCCGGA 1 cut(s) 123
BspFNI CGCG 2 cut(s) 771, 1014
BspHI TCATGA 3 cut(s) 318, 337, 499
BspLI GGNNCC 2 cut(s) 172, 737
BspOI GCTAGC 1 cut(s) 704
BspPI GGATC 2 cut(s) 290, 316
BsrDI GCAATG 2 cut(s) 274, 761
BsrI ACTGG 3 cut(s) 325, 807, 892
BssECI CCNNGG 2 cut(s) 368, 1031
BssMI GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
BssSI CACGAG 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 368
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 1 cut(s) 928
BstC8I GCNNGC 4 cut(s) 622, 702, 947, 999
BstDEI CTNAG 4 cut(s) 90, 114, 533, 643
BstDSI CCRYGG 1 cut(s) 1031
BstF5I GGATG 3 cut(s) 835, 1062, 1120
BstFNI CGCG 2 cut(s) 771, 1014
BstHHI GCGC 2 cut(s) 956, 1014
BstKTI GATC 7 cut(s) 10, 256, 286, 298, 324, 343, 529
BstMAI GTCTC 3 cut(s) 121, 166, 390
BstMBI GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
BstMWI GCNNNNNNNGC 1 cut(s) 951
BstNI CCWGG 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 209, 975
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 2 cut(s) 771, 1014
BstV1I GCAGC 1 cut(s) 83
BstXI CCANNNNNNTGG 1 cut(s) 899
BsuRI GGCC 1 cut(s) 1001
BtgI CCRYGG 1 cut(s) 1031
BtsCI GGATG 3 cut(s) 835, 1062, 1120
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 2 cut(s) 590, 924
BtuMI TCGCGA 1 cut(s) 771
Cac8I GCNNGC 4 cut(s) 622, 702, 947, 999
CciI TCATGA 3 cut(s) 318, 337, 499
CfoI GCGC 2 cut(s) 956, 1014
Cfr13I GGNCC 1 cut(s) 170
CviAII CATG 9 cut(s) 11, 52, 310, 319, 338, 500, 599, 764, 984
DdeI CTNAG 4 cut(s) 90, 114, 533, 643
DpnI GATC 7 cut(s) 9, 255, 285, 297, 323, 342, 528
DpnII GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
EaeI YGGCCR 1 cut(s) 999
Eco130I CCWWGG 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 4 cut(s) 88, 430, 900, 978
Eco88I CYCGRG 1 cut(s) 380
EcoRI GAATTC 3 cut(s) 73, 272, 629
EcoRII CCWGG 1 cut(s) 975
EcoT14I CCWWGG 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 368
FaeI CATG 9 cut(s) 14, 55, 313, 322, 341, 503, 602, 767, 987
FalI AAGNNNNNCTT 2 cut(s) 48, 80
FaqI GGGAC 2 cut(s) 401, 451
FatI CATG 9 cut(s) 10, 51, 309, 318, 337, 499, 598, 763, 983
FauI CCCGC 1 cut(s) 938
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 3 cut(s) 842, 1049, 1107
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 2 cut(s) 353, 701
GlaI GCGC 2 cut(s) 955, 1013
GluI GCNGC 1 cut(s) 97
HaeIII GGCC 1 cut(s) 1001
HapII CCGG 3 cut(s) 124, 210, 1002
HhaI GCGC 2 cut(s) 956, 1014
Hin1II CATG 9 cut(s) 14, 55, 313, 322, 341, 503, 602, 767, 987
Hin6I GCGC 2 cut(s) 954, 1012
HinP1I GCGC 2 cut(s) 954, 1012
HindIII AAGCTT 2 cut(s) 62, 905
HinfI GANTC 5 cut(s) 245, 278, 383, 511, 569
HpaII CCGG 3 cut(s) 124, 210, 1002
HphI GGTGA 2 cut(s) 173, 1072
Hpy188I TCNGA 4 cut(s) 107, 170, 283, 635
Hpy188III TCNNGA 8 cut(s) 113, 124, 319, 338, 380, 500, 733, 770
HpyAV CCTTC 3 cut(s) 757, 1069, 1076
HpyCH4III ACNGT 1 cut(s) 928
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 7 cut(s) 69, 99, 242, 477, 590, 794, 1090
HpyF10VI GCNNNNNNNGC 1 cut(s) 951
HpyF3I CTNAG 4 cut(s) 90, 114, 533, 643
HpySE526I ACGT 1 cut(s) 223
Hsp92II CATG 9 cut(s) 14, 55, 313, 322, 341, 503, 602, 767, 987
HspAI GCGC 2 cut(s) 954, 1012
Kpn2I TCCGGA 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 1 cut(s) 108
MaeI CTAG 2 cut(s) 353, 701
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 4 cut(s) 117, 781, 845, 914
MalI GATC 7 cut(s) 9, 255, 285, 297, 323, 342, 528
MboI GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
MboII GAAGA 7 cut(s) 17, 94, 335, 436, 648, 722, 893
MfeI CAATTG 2 cut(s) 705, 865
MhlI GDGCHC 2 cut(s) 229, 438
MluCI AATT 8 cut(s) 73, 272, 563, 629, 690, 705, 865, 967
MlyI GAGTC 2 cut(s) 239, 392
MmeI TCCRAC 1 cut(s) 910
MnlI CCTC 7 cut(s) 133, 185, 431, 499, 610, 749, 1134
MroI TCCGGA 1 cut(s) 123
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 261, 498
MspI CCGG 3 cut(s) 124, 210, 1002
MspR9I CCNGG 2 cut(s) 211, 977
MunI CAATTG 2 cut(s) 705, 865
MvaI CCWGG 1 cut(s) 977
MvnI CGCG 2 cut(s) 771, 1014
MwoI GCNNNNNNNGC 1 cut(s) 951
NciI CCSGG 1 cut(s) 211
NdeII GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
NheI GCTAGC 1 cut(s) 700
NlaIII CATG 9 cut(s) 14, 55, 313, 322, 341, 503, 602, 767, 987
NlaIV GGNNCC 2 cut(s) 172, 737
NmuCI GTSAC 2 cut(s) 845, 914
NruI TCGCGA 1 cut(s) 771
PaeR7I CTCGAG 1 cut(s) 380
PagI TCATGA 3 cut(s) 318, 337, 499
PfeI GAWTC 3 cut(s) 278, 511, 569
PkrI GCNGC 1 cut(s) 98
PleI GAGTC 2 cut(s) 239, 391
PpsI GAGTC 2 cut(s) 239, 391
Psp6I CCWGG 1 cut(s) 975
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 2 cut(s) 172, 737
PspPI GGNCC 1 cut(s) 170
RruI TCGCGA 1 cut(s) 771
RseI CAYNNNNRTG 2 cut(s) 261, 498
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 7 cut(s) 7, 253, 283, 295, 321, 340, 526
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 2 cut(s) 239, 392
ScrFI CCNGG 2 cut(s) 211, 977
SduI GDGCHC 2 cut(s) 229, 438
SfaNI GCATC 1 cut(s) 108
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 2 cut(s) 261, 498
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 8 cut(s) 73, 272, 563, 629, 690, 705, 865, 967
SsiI CCGC 1 cut(s) 945
SspI AATATT 2 cut(s) 25, 664
SspMI CTAG 2 cut(s) 353, 701
StyD4I CCNGG 2 cut(s) 209, 975
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 1 cut(s) 928
TaiI ACGT 1 cut(s) 226
TaqI TCGA 3 cut(s) 276, 381, 750
TasI AATT 8 cut(s) 73, 272, 563, 629, 690, 705, 865, 967
TfiI GAWTC 3 cut(s) 278, 511, 569
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 597, 931
TseFI GTSAC 2 cut(s) 845, 914
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 2 cut(s) 845, 914
TspDTI ATGAA 6 cut(s) 285, 307, 326, 674, 899, 1051
TspGWI ACGGA 2 cut(s) 61, 1020
TspRI CASTG 2 cut(s) 597, 931
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 4 cut(s) 73, 272, 563, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 380
XspI CTAG 2 cut(s) 353, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.