Rroxscaffold_2G00077520

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000188
Physical Location & Seq
Reverse (-)
1027819 .. 1029277
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077520.1

Sequence Viewer

Length: 756 bp
ATGGGAGGTAGCAGTGATATAGAAAAGGGTGAAGCGGATGAAAGCGTTCCAGTTCATCTAGAACGTAAAGTGGCTGATGCCAAAATTTTGAGGAACCCCGATCCGGAGTCTTCTGGTACGAATCTTCACATACTTGATTTGATTAGAACTGCCCTCGTTCTTGGCCCAGGTTCGAAAGCTGCCGCCACAACAGACATCCACCTGCCGCAAAGGATACCTAGTGCTGCTACTCTTTCAGAGGCAGGCGTTATATTTAAAAAGGCCAAAGAAAGTTGCATATTGAACATAACGTTCAACAATGGCGTTTTCACCATTCCTCCGTTGGCTATCGATGAGAGTACTGGTCCTTTGTTCAGGAACCTTATGGCCTTCGAGCAGTGCTATCACAGTTGCGAGCACAGGATAACATCCTATGCGGTTTTGATGGATAGCCTCATCGATACGAACAAGGATGTGGATCTTCTATGTGAGGAAGGGATAATAGCCAACTGGTTGAGTGCTGATGATGCTTCGAAGTTCTTCAACGATCTATACACTGACACCACAGTCATTGATTTTGTCTACGAGGATCTCTGCAAAAGAGTGAACGAATATCACAAAGCTCCATGCAACAGGTGGAGAGAAAAATTGAAACATGATTATTTTGATACACCATGGTCTACTATATCTTTTTTTGCAGCTTTTGTCCTCCTGGTCCTCACCTTCTTGCAGACCATATACACAATCCAGCCATACTATCATCCCGCTCCTGAATAA

Protein Analysis

251

Amino Acids

28.15

Weight (kDa)

5.0

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 34 - 233 3.7e-62 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000188)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02500 FvH4_4g03110 FvH4_5g11900 FvH4_5g11910 FvH4_5g19510 FvH4_6g53141 FvH4_6g53141 FvH4_6g53143 FvH4_6g53143 FvH4_6g53260 FvH4_6g53260 FvH4_6g53260 FvH4_6g53280
malus_domestica MD00G1110800.v1.1 MD00G1111300.v1.1 MD08G1225300.v1.1 MD08G1225500.v1.1 MD08G1225700.v1.1 MD09G1013500.v1.1 MD09G1013700.v1.1 MD09G1013800.v1.1 MD09G1014000.v1.1
prunus_persica Prupe.1G028300_v2.0.a1 Prupe.1G029300_v2.0.a1 Prupe.1G029400_v2.0.a1 Prupe.1G029800_v2.0.a1 Prupe.1G165900_v2.0.a1 Prupe.3G303500_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029200_v2.0.a1 Prupe.7G037400_v2.0.a1
pyrus_communis pycom08g19660 pycom08g19690 pycom08g19710 pycom08g19720 pycom111g01160 pycom111g01170 pycom17g01140
rosa_chinensis RchiOBHm_Chr2g0111831 RchiOBHm_Chr2g0138211 RchiOBHm_Chr2g0163091 RchiOBHm_Chr2g0175051 RchiOBHm_Chr2g0175141 RchiOBHm_Chr2g0175171 RchiOBHm_Chr2g0175191 RchiOBHm_Chr3g0483151 RchiOBHm_Chr3g0483161 RchiOBHm_Chr3g0483171 RchiOBHm_Chr4g0389941 RchiOBHm_Chr4g0411021 RchiOBHm_Chr4g0411031 RchiOBHm_Chr4g0411041 RchiOBHm_Chr5g0041861 RchiOBHm_Chr5g0058261 RchiOBHm_Chr5g0065661 RchiOBHm_Chr5g0070431 RchiOBHm_Chr7g0185221 RchiOBHm_Chr7g0185581 RchiOBHm_Chr7g0185591 RchiOBHm_Chr7g0185611 RchiOBHm_Chr7g0185641
rosa_laevigata RLG00000004895 RLG00000004896 RLG00000004897 RLG00000008379 RLG00000021370 RLG00000022309 RLG00000034099 RLG00000035811 RLG00000036132 RLG00000036450
rosa_multiflora Rmu_co8229993.1_g000001 Rmu_co8360165.1_g000001 Rmu_co8461695.1_g000001 Rmu_sc0000213.1_g000004 Rmu_sc0000235.1_g000066 Rmu_sc0000367.1_g000060 Rmu_sc0000861.1_g000057 Rmu_sc0000861.1_g000068 Rmu_sc0001244.1_g000011 Rmu_sc0001244.1_g000013 Rmu_sc0001323.1_g000018 Rmu_sc0002180.1_g000032 Rmu_sc0002280.1_g000009 Rmu_sc0003226.1_g000095 Rmu_sc0003226.1_g000096 Rmu_sc0003555.1_g000010 Rmu_sc0003555.1_g000018 Rmu_sc0003920.1_g000011 Rmu_sc0003984.1_g000012 Rmu_sc0004336.1_g000026 Rmu_sc0006027.1_g000005 Rmu_sc0006570.1_g000004 Rmu_sc0007961.1_g000010 Rmu_sc0008747.1_g000001 Rmu_sc0008834.1_g000005 Rmu_sc0010190.1_g000004 Rmu_sc0010190.1_g000007 Rmu_sc0010190.1_g000008 Rmu_sc0010642.1_g000012 Rmu_sc0011497.1_g000002
rosa_roxburghii Rroxscaffold_1G00010770 Rroxscaffold_1G00010780 Rroxscaffold_1G00011860 Rroxscaffold_1G00015470 Rroxscaffold_1G00038670 Rroxscaffold_2G00077520 Rroxscaffold_2G00077550 Rroxscaffold_2G00106420 Rroxscaffold_3G00268750 Rroxscaffold_3G00268770 Rroxscaffold_3G00268780 Rroxscaffold_3G00268790 Rroxscaffold_3G00269130 Rroxscaffold_5G00336020 Rroxscaffold_5G00343150 Rroxscaffold_5G00355700
rosa_rugosa Rorug02G0500300 Rorug02G0581200 Rorug03G0204700 Rorug03G0327300 Rorug05G0196100 Rorug05G0196400 Rorug05G0405900 Rorug05G0406000 Rorug06G0467000 Rorug06G0467000 Rorug06G0470200 Rorug06G0470200 Rorug07G0180500
rosa_samantha Rh2AG566700 Rh2AG661900 Rh2AG663200 Rh3CG286400 Rh3CG286500 Rh3CG366100 Rh4DG024200 Rh4DG076600 Rh4DG160000 Rh4DG160100 Rh5BG286800 Rh5DG295500 Rh5DG461000 Rh7DG072100 Rh7DG075500
rosa_wichuraiana Rw1G002310 Rw1G015820 Rw2G046920 Rw2G054280 Rw3G022880 Rw3G029170 Rw4G002330 Rw4G013670 Rw5G026420 Rw5G026440 Rw5G040490 Rw5G042130 Rw7G006300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 210
AasI GACNNNNNNGTC 1 cut(s) 545
Acc36I ACCTGC 1 cut(s) 210
AccBSI CCGCTC 1 cut(s) 746
AccI GTMKAC 2 cut(s) 561, 659
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 5 cut(s) 35, 183, 206, 416, 744
AclI AACGTT 1 cut(s) 290
AclWI GGATC 3 cut(s) 95, 465, 576
AcsI RAATTY 1 cut(s) 84
AfaI GTAC 2 cut(s) 118, 340
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 4 cut(s) 283, 295, 523, 631
AjnI CCWGG 2 cut(s) 166, 690
AluBI AGCT 3 cut(s) 179, 602, 680
AluI AGCT 3 cut(s) 179, 602, 680
Alw21I GWGCWC 1 cut(s) 399
AlwI GGATC 3 cut(s) 95, 465, 576
Aor13HI TCCGGA 1 cut(s) 103
AoxI GGCC 3 cut(s) 163, 261, 366
ApeKI GCWGC 3 cut(s) 179, 224, 677
ApoI RAATTY 1 cut(s) 84
Asp700I GAANNNNTTC 2 cut(s) 45, 518
AspS9I GGNCC 3 cut(s) 164, 344, 694
AsuHPI GGTGA 3 cut(s) 41, 301, 691
AsuII TTCGAA 2 cut(s) 173, 512
AvaII GGWCC 2 cut(s) 344, 694
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 166, 211, 689
BccI CCATC 1 cut(s) 418
BciT130I CCWGG 2 cut(s) 168, 692
BciVI GTATCC 1 cut(s) 207
BfaI CTAG 2 cut(s) 59, 219
BfuAI ACCTGC 1 cut(s) 210
BfuI GTATCC 1 cut(s) 207
BisI GCNGC 5 cut(s) 180, 183, 206, 225, 678
BlsI GCNGC 5 cut(s) 181, 184, 207, 226, 679
BmcAI AGTACT 1 cut(s) 340
Bme1390I CCNGG 2 cut(s) 168, 692
Bme18I GGWCC 2 cut(s) 344, 694
BmgT120I GGNCC 3 cut(s) 164, 344, 694
BmiI GGNNCC 2 cut(s) 95, 359
BmrFI CCNGG 2 cut(s) 168, 692
BmsI GCATC 2 cut(s) 67, 496
BpiI GAAGAC 1 cut(s) 102
Bpu14I TTCGAA 2 cut(s) 173, 512
Bsa29I ATCGAT 2 cut(s) 330, 438
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 166, 653
BsaWI WCCGGW 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 301, 331
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 50, 346, 494
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 103
BseBI CCWGG 2 cut(s) 168, 692
BseCI ATCGAT 2 cut(s) 330, 438
BseDI CCNNGG 2 cut(s) 166, 653
BseGI GGATG 5 cut(s) 43, 195, 407, 457, 739
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 3 cut(s) 50, 346, 494
BseXI GCAGC 3 cut(s) 166, 211, 689
BshFI GGCC 3 cut(s) 165, 263, 368
BshVI ATCGAT 2 cut(s) 330, 438
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 103
BsnI GGCC 3 cut(s) 165, 263, 368
Bsp119I TTCGAA 2 cut(s) 173, 512
Bsp1286I GDGCHC 1 cut(s) 399
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 4 cut(s) 100, 457, 526, 568
Bsp19I CCATGG 1 cut(s) 653
BspACI CCGC 5 cut(s) 35, 183, 206, 416, 744
BspANI GGCC 3 cut(s) 165, 263, 368
BspDI ATCGAT 2 cut(s) 330, 438
BspEI TCCGGA 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 95, 359
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 3 cut(s) 95, 465, 576
BspT104I TTCGAA 2 cut(s) 173, 512
BsrBI CCGCTC 1 cut(s) 746
BsrI ACTGG 3 cut(s) 50, 346, 494
BssECI CCNNGG 2 cut(s) 166, 653
BssMI GATC 4 cut(s) 100, 457, 526, 568
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 2 cut(s) 168, 692
Bst4CI ACNGT 2 cut(s) 389, 547
BstBI TTCGAA 2 cut(s) 173, 512
BstC8I GCNNGC 2 cut(s) 244, 395
BstDSI CCRYGG 1 cut(s) 653
BstF5I GGATG 5 cut(s) 43, 195, 407, 457, 739
BstKTI GATC 4 cut(s) 103, 460, 529, 571
BstMBI GATC 4 cut(s) 100, 457, 526, 568
BstMWI GCNNNNNNNGC 1 cut(s) 506
BstNI CCWGG 2 cut(s) 168, 692
BstSCI CCNGG 2 cut(s) 166, 690
BstV1I GCAGC 3 cut(s) 166, 211, 689
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 2 cut(s) 457, 568
BstYI RGATCY 2 cut(s) 457, 568
Bsu15I ATCGAT 2 cut(s) 330, 438
BsuI GTATCC 1 cut(s) 207
BsuRI GGCC 3 cut(s) 165, 263, 368
BsuTUI ATCGAT 2 cut(s) 330, 438
BtgI CCRYGG 1 cut(s) 653
BtsCI GGATG 5 cut(s) 43, 195, 407, 457, 739
BtsI GCAGTG 2 cut(s) 19, 383
BtsIMutI CAGTG 3 cut(s) 19, 383, 534
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 244, 395
Cfr13I GGNCC 3 cut(s) 164, 344, 694
ClaI ATCGAT 2 cut(s) 330, 438
Csp6I GTAC 2 cut(s) 117, 339
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 3 cut(s) 606, 635, 654
CviQI GTAC 2 cut(s) 117, 339
DpnI GATC 4 cut(s) 102, 459, 528, 570
DpnII GATC 4 cut(s) 100, 457, 526, 568
DraI TTTAAA 1 cut(s) 256
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 653
Eco47I GGWCC 2 cut(s) 344, 694
EcoRII CCWGG 2 cut(s) 166, 690
EcoT14I CCWWGG 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 3 cut(s) 609, 638, 657
FatI CATG 3 cut(s) 605, 634, 653
FauI CCCGC 1 cut(s) 751
FblI GTMKAC 2 cut(s) 561, 659
Fnu4HI GCNGC 5 cut(s) 180, 183, 206, 225, 678
FokI GGATG 5 cut(s) 50, 182, 394, 464, 726
Fsp4HI GCNGC 5 cut(s) 180, 183, 206, 225, 678
FspBI CTAG 2 cut(s) 59, 219
GluI GCNGC 5 cut(s) 180, 183, 206, 225, 678
HaeIII GGCC 3 cut(s) 165, 263, 368
HapII CCGG 1 cut(s) 104
Hin1II CATG 3 cut(s) 609, 638, 657
HinfI GANTC 2 cut(s) 107, 121
HpaII CCGG 1 cut(s) 104
HphI GGTGA 3 cut(s) 41, 301, 691
Hpy166II GTNNAC 3 cut(s) 562, 586, 660
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 4 cut(s) 59, 104, 355, 749
Hpy8I GTNNAC 3 cut(s) 562, 586, 660
HpyAV CCTTC 3 cut(s) 379, 467, 712
HpyCH4III ACNGT 2 cut(s) 389, 547
HpyCH4IV ACGT 2 cut(s) 64, 290
HpyCH4V TGCA 5 cut(s) 276, 576, 609, 677, 709
HpyF10VI GCNNNNNNNGC 1 cut(s) 506
HpySE526I ACGT 2 cut(s) 64, 290
Hsp92II CATG 3 cut(s) 609, 638, 657
Kpn2I TCCGGA 1 cut(s) 103
Kzo9I GATC 4 cut(s) 100, 457, 526, 568
LmnI GCTCC 2 cut(s) 607, 751
Lsp1109I GCAGC 3 cut(s) 166, 211, 689
LweI GCATC 2 cut(s) 67, 496
MaeI CTAG 2 cut(s) 59, 219
MaeII ACGT 2 cut(s) 64, 290
MalI GATC 4 cut(s) 102, 459, 528, 570
MbiI CCGCTC 1 cut(s) 746
MboI GATC 4 cut(s) 100, 457, 526, 568
MboII GAAGA 4 cut(s) 102, 116, 452, 511
MflI RGATCY 2 cut(s) 457, 568
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 2 cut(s) 84, 626
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 9 cut(s) 84, 164, 232, 327, 443, 463, 559, 698, 707
MroI TCCGGA 1 cut(s) 103
MroXI GAANNNNTTC 2 cut(s) 45, 518
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 168, 692
MvaI CCWGG 2 cut(s) 168, 692
MwoI GCNNNNNNNGC 1 cut(s) 506
NcoI CCATGG 1 cut(s) 653
NdeII GATC 4 cut(s) 100, 457, 526, 568
NlaIII CATG 3 cut(s) 609, 638, 657
NlaIV GGNNCC 2 cut(s) 95, 359
NspV TTCGAA 2 cut(s) 173, 512
PaqCI CACCTGC 1 cut(s) 210
PdmI GAANNNNTTC 2 cut(s) 45, 518
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 5 cut(s) 181, 184, 207, 226, 679
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp1406I AACGTT 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 166, 690
PspGI CCWGG 2 cut(s) 166, 690
PspN4I GGNNCC 2 cut(s) 95, 359
PspPI GGNCC 3 cut(s) 164, 344, 694
PsuI RGATCY 2 cut(s) 457, 568
RsaI GTAC 2 cut(s) 118, 340
RsaNI GTAC 2 cut(s) 117, 339
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 5 cut(s) 180, 183, 206, 225, 678
Sau3AI GATC 4 cut(s) 100, 457, 526, 568
Sau96I GGNCC 3 cut(s) 164, 344, 694
ScaI AGTACT 1 cut(s) 340
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 168, 692
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 2 cut(s) 67, 496
SfuI TTCGAA 2 cut(s) 173, 512
SinI GGWCC 2 cut(s) 344, 694
Sse9I AATT 2 cut(s) 84, 626
SsiI CCGC 5 cut(s) 35, 183, 206, 416, 744
SspMI CTAG 2 cut(s) 59, 219
StyD4I CCNGG 2 cut(s) 166, 690
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 2 cut(s) 389, 547
TaiI ACGT 2 cut(s) 67, 293
TaqI TCGA 5 cut(s) 173, 330, 372, 438, 512
TasI AATT 2 cut(s) 84, 626
TatI WGTACW 1 cut(s) 338
TauI GCSGC 2 cut(s) 185, 208
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 3 cut(s) 19, 383, 541
TseI GCWGC 3 cut(s) 179, 224, 677
TspDTI ATGAA 2 cut(s) 44, 54
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 3 cut(s) 19, 383, 541
VpaK11BI GGWCC 2 cut(s) 344, 694
XapI RAATTY 1 cut(s) 84
XbaI TCTAGA 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 612
XmiI GTMKAC 2 cut(s) 561, 659
XmnI GAANNNNTTC 2 cut(s) 45, 518
XspI CTAG 2 cut(s) 59, 219
ZrmI AGTACT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.