MD08G1225500.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000188
Physical Location & Seq
Reverse (-)
29236658 .. 29237446
789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1225500.v1.1.491

Sequence Viewer

Length: 789 bp
ATGTATGAGCTCACCGTGAAGTATTACCAGAACCATGAGCGCTGCCTCTCGGAACTCATCCTCACCGCGCTCAGCTCACAACCACAACTGTCCCATAACTGCCGGTGGTATCTGGATCATCTAAGAGGAAATAGTTACAAAGAGGACGAAAGTGTGCTCCACATTCTCGATCTCATAAGAACTTCCATAGTTTTCTCATTCACCGAACGCTATGAGAAACCTCCCTTCGATGCAAAGATACAACTGATAGCTTCAGCAACTGCTCTTTCTGAGGCCGGCATTAGATTCAAAATGAGCTCCGATGAATGTAAAAGCATAATGAACATCGAATGCGAGAATGGGGTTATTATGATTCCACATCTAGAAATTGGAGAGTTAACTGAATCGATATTCAGGAACCTGATCGCCTTGGAGCAATGCTGTTATGGTCGTTCGCATAAAATCACATCTTATGCCGTTCTGATGGATAACCTCCTCGGCTCTAGCAACGATATAAAATTTCTCTGCGACAAGGAAATTATAGGTAACTGGCTGAGCGCTGAAACTGGTTCTGAGTTCTTCAGCAAGCTTTACTGCGACACGGTGCTTACTTATTTCTACTATGGTGGACTCTGTACTCGAGTGAAGGAATACCAAACTGCATTGAGGAGGTGGCGAGAAATATTCAAGCGTAACTATTGGTCAGATCCATGGAAAGCCACCTCCTGGACTGTAGCAAGTATCCTTCTGATTCTCACCTTAGTGCAGACTGCATACACCATTAAGCAATACTATTCTCCTCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

263

Amino Acids

30.45

Weight (kDa)

6.1

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 42 - 245 1e-48 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000188)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02500 FvH4_4g03110 FvH4_5g11900 FvH4_5g11910 FvH4_5g19510 FvH4_6g53141 FvH4_6g53141 FvH4_6g53143 FvH4_6g53143 FvH4_6g53260 FvH4_6g53260 FvH4_6g53260 FvH4_6g53280
malus_domestica MD00G1110800.v1.1 MD00G1111300.v1.1 MD08G1225300.v1.1 MD08G1225500.v1.1 MD08G1225700.v1.1 MD09G1013500.v1.1 MD09G1013700.v1.1 MD09G1013800.v1.1 MD09G1014000.v1.1
prunus_persica Prupe.1G028300_v2.0.a1 Prupe.1G029300_v2.0.a1 Prupe.1G029400_v2.0.a1 Prupe.1G029800_v2.0.a1 Prupe.1G165900_v2.0.a1 Prupe.3G303500_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029200_v2.0.a1 Prupe.7G037400_v2.0.a1
pyrus_communis pycom08g19660 pycom08g19690 pycom08g19710 pycom08g19720 pycom111g01160 pycom111g01170 pycom17g01140
rosa_chinensis RchiOBHm_Chr2g0111831 RchiOBHm_Chr2g0138211 RchiOBHm_Chr2g0163091 RchiOBHm_Chr2g0175051 RchiOBHm_Chr2g0175141 RchiOBHm_Chr2g0175171 RchiOBHm_Chr2g0175191 RchiOBHm_Chr3g0483151 RchiOBHm_Chr3g0483161 RchiOBHm_Chr3g0483171 RchiOBHm_Chr4g0389941 RchiOBHm_Chr4g0411021 RchiOBHm_Chr4g0411031 RchiOBHm_Chr4g0411041 RchiOBHm_Chr5g0041861 RchiOBHm_Chr5g0058261 RchiOBHm_Chr5g0065661 RchiOBHm_Chr5g0070431 RchiOBHm_Chr7g0185221 RchiOBHm_Chr7g0185581 RchiOBHm_Chr7g0185591 RchiOBHm_Chr7g0185611 RchiOBHm_Chr7g0185641
rosa_laevigata RLG00000004895 RLG00000004896 RLG00000004897 RLG00000008379 RLG00000021370 RLG00000022309 RLG00000034099 RLG00000035811 RLG00000036132 RLG00000036450
rosa_multiflora Rmu_co8229993.1_g000001 Rmu_co8360165.1_g000001 Rmu_co8461695.1_g000001 Rmu_sc0000213.1_g000004 Rmu_sc0000235.1_g000066 Rmu_sc0000367.1_g000060 Rmu_sc0000861.1_g000057 Rmu_sc0000861.1_g000068 Rmu_sc0001244.1_g000011 Rmu_sc0001244.1_g000013 Rmu_sc0001323.1_g000018 Rmu_sc0002180.1_g000032 Rmu_sc0002280.1_g000009 Rmu_sc0003226.1_g000095 Rmu_sc0003226.1_g000096 Rmu_sc0003555.1_g000010 Rmu_sc0003555.1_g000018 Rmu_sc0003920.1_g000011 Rmu_sc0003984.1_g000012 Rmu_sc0004336.1_g000026 Rmu_sc0006027.1_g000005 Rmu_sc0006570.1_g000004 Rmu_sc0007961.1_g000010 Rmu_sc0008747.1_g000001 Rmu_sc0008834.1_g000005 Rmu_sc0010190.1_g000004 Rmu_sc0010190.1_g000007 Rmu_sc0010190.1_g000008 Rmu_sc0010642.1_g000012 Rmu_sc0011497.1_g000002
rosa_roxburghii Rroxscaffold_1G00010770 Rroxscaffold_1G00010780 Rroxscaffold_1G00011860 Rroxscaffold_1G00015470 Rroxscaffold_1G00038670 Rroxscaffold_2G00077520 Rroxscaffold_2G00077550 Rroxscaffold_2G00106420 Rroxscaffold_3G00268750 Rroxscaffold_3G00268770 Rroxscaffold_3G00268780 Rroxscaffold_3G00268790 Rroxscaffold_3G00269130 Rroxscaffold_5G00336020 Rroxscaffold_5G00343150 Rroxscaffold_5G00355700
rosa_rugosa Rorug02G0500300 Rorug02G0581200 Rorug03G0204700 Rorug03G0327300 Rorug05G0196100 Rorug05G0196400 Rorug05G0405900 Rorug05G0406000 Rorug06G0467000 Rorug06G0467000 Rorug06G0470200 Rorug06G0470200 Rorug07G0180500
rosa_samantha Rh2AG566700 Rh2AG661900 Rh2AG663200 Rh3CG286400 Rh3CG286500 Rh3CG366100 Rh4DG024200 Rh4DG076600 Rh4DG160000 Rh4DG160100 Rh5BG286800 Rh5DG295500 Rh5DG461000 Rh7DG072100 Rh7DG075500
rosa_wichuraiana Rw1G002310 Rw1G015820 Rw2G046920 Rw2G054280 Rw3G022880 Rw3G029170 Rw4G002330 Rw4G013670 Rw5G026420 Rw5G026440 Rw5G040490 Rw5G042130 Rw7G006300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 705
AccII CGCG 1 cut(s) 68
AciI CCGC 1 cut(s) 66
AclWI GGATC 2 cut(s) 123, 680
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 2 cut(s) 237, 544
AfaI GTAC 1 cut(s) 616
AfeI AGCGCT 2 cut(s) 41, 538
AfiI CCNNNNNNNGG 1 cut(s) 705
AgsI TTSAA 2 cut(s) 289, 667
AjnI CCWGG 1 cut(s) 704
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 5 cut(s) 10, 75, 251, 297, 568
AluI AGCT 5 cut(s) 10, 75, 251, 297, 568
Alw21I GWGCWC 3 cut(s) 12, 159, 299
AlwI GGATC 2 cut(s) 123, 680
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 618
Aor51HI AGCGCT 2 cut(s) 41, 538
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 42
ApoI RAATTY 1 cut(s) 497
AspLEI GCGC 3 cut(s) 42, 70, 539
AsuHPI GGTGA 4 cut(s) 4, 55, 193, 727
AvaI CYCGRG 1 cut(s) 618
AxyI CCTNAGG 1 cut(s) 780
BanII GRGCYC 2 cut(s) 12, 299
Bbv12I GWGCWC 3 cut(s) 12, 159, 299
BbvI GCAGC 1 cut(s) 29
BccI CCATC 1 cut(s) 457
BceAI ACGGC 1 cut(s) 440
BciT130I CCWGG 1 cut(s) 706
BciVI GTATCC 1 cut(s) 731
BfaI CTAG 2 cut(s) 362, 483
BfmI CTRYAG 1 cut(s) 711
BfoI RGCGCY 2 cut(s) 43, 540
BfuI GTATCC 1 cut(s) 731
BisI GCNGC 1 cut(s) 43
BlpI GCTNAGC 2 cut(s) 71, 533
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 706
BmeT110I CYCGRG 1 cut(s) 618
BmiI GGNNCC 1 cut(s) 398
BmrFI CCNGG 1 cut(s) 706
BmsI GCATC 1 cut(s) 220
Bpu1102I GCTNAGC 2 cut(s) 71, 533
Bsa29I ATCGAT 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 408, 475, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 705
Bse118I RCCGGY 2 cut(s) 102, 275
Bse1I ACTGG 2 cut(s) 533, 550
Bse21I CCTNAGG 1 cut(s) 780
Bse3DI GCAATG 1 cut(s) 422
BseBI CCWGG 1 cut(s) 706
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 408, 475, 689
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 705
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 4 cut(s) 85, 261, 524, 543
BseNI ACTGG 2 cut(s) 533, 550
BseRI GAGGAG 3 cut(s) 464, 661, 768
BseXI GCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 764
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 386
BsiHKAI GWGCWC 3 cut(s) 12, 159, 299
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 2 cut(s) 103, 276
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 3 cut(s) 12, 159, 299
Bsp143I GATC 4 cut(s) 115, 169, 402, 685
Bsp1720I GCTNAGC 2 cut(s) 71, 533
Bsp19I CCATGG 1 cut(s) 689
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 4 cut(s) 84, 262, 525, 544
BspDI ATCGAT 1 cut(s) 386
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 398
BspPI GGATC 2 cut(s) 123, 680
BsrDI GCAATG 1 cut(s) 422
BsrFI RCCGGY 2 cut(s) 102, 275
BsrI ACTGG 2 cut(s) 533, 550
BssAI RCCGGY 2 cut(s) 102, 275
BssECI CCNNGG 3 cut(s) 408, 475, 689
BssMI GATC 4 cut(s) 115, 169, 402, 685
BssT1I CCWWGG 2 cut(s) 408, 689
Bst2UI CCWGG 1 cut(s) 706
Bst4CI ACNGT 4 cut(s) 16, 90, 583, 712
BstC8I GCNNGC 2 cut(s) 277, 566
BstDEI CTNAG 7 cut(s) 71, 122, 270, 533, 552, 739, 780
BstDSI CCRYGG 1 cut(s) 689
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 2 cut(s) 43, 540
BstHHI GCGC 3 cut(s) 42, 70, 539
BstKTI GATC 4 cut(s) 118, 172, 405, 688
BstMBI GATC 4 cut(s) 115, 169, 402, 685
BstNI CCWGG 1 cut(s) 706
BstSCI CCNGG 1 cut(s) 704
BstSFI CTRYAG 1 cut(s) 711
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
Bsu15I ATCGAT 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 780
BsuI GTATCC 1 cut(s) 731
BsuRI GGCC 1 cut(s) 275
BsuTUI ATCGAT 1 cut(s) 386
BtgI CCRYGG 1 cut(s) 689
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 277, 566
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 3 cut(s) 42, 70, 539
Cfr10I RCCGGY 2 cut(s) 102, 275
ClaI ATCGAT 1 cut(s) 386
Csp6I GTAC 1 cut(s) 615
CviAII CATG 2 cut(s) 35, 690
CviJI RGCY 9 cut(s) 10, 75, 251, 275, 297, 480, 532, 568, 698
CviKI_1 RGCY 9 cut(s) 10, 75, 251, 275, 297, 480, 532, 568, 698
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 7 cut(s) 71, 122, 270, 533, 552, 739, 780
DpnI GATC 4 cut(s) 117, 171, 404, 687
DpnII GATC 4 cut(s) 115, 169, 402, 685
Ecl136II GAGCTC 2 cut(s) 10, 297
Eco130I CCWWGG 2 cut(s) 408, 689
Eco24I GRGCYC 2 cut(s) 12, 299
Eco47III AGCGCT 2 cut(s) 41, 538
Eco53kI GAGCTC 2 cut(s) 10, 297
Eco57I CTGAAG 2 cut(s) 237, 544
Eco81I CCTNAGG 1 cut(s) 780
Eco88I CYCGRG 1 cut(s) 618
EcoICRI GAGCTC 2 cut(s) 10, 297
EcoRII CCWGG 1 cut(s) 704
EcoT14I CCWWGG 2 cut(s) 408, 689
EcoT38I GRGCYC 2 cut(s) 12, 299
ErhI CCWWGG 2 cut(s) 408, 689
FaeI CATG 2 cut(s) 38, 693
FaqI GGGAC 1 cut(s) 76
FatI CATG 2 cut(s) 34, 689
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 2 cut(s) 12, 299
Fsp4HI GCNGC 1 cut(s) 43
FspBI CTAG 2 cut(s) 362, 483
GlaI GCGC 3 cut(s) 41, 69, 538
GluI GCNGC 1 cut(s) 43
HaeII RGCGCY 2 cut(s) 43, 540
HaeIII GGCC 1 cut(s) 275
HapII CCGG 2 cut(s) 103, 276
HhaI GCGC 3 cut(s) 42, 70, 539
Hin1II CATG 2 cut(s) 38, 693
Hin6I GCGC 3 cut(s) 40, 68, 537
HinP1I GCGC 3 cut(s) 40, 68, 537
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 5 cut(s) 285, 352, 383, 609, 730
HpaI GTTAAC 1 cut(s) 378
HpaII CCGG 2 cut(s) 103, 276
HphI GGTGA 4 cut(s) 4, 55, 193, 727
Hpy166II GTNNAC 2 cut(s) 378, 608
Hpy188I TCNGA 7 cut(s) 52, 271, 301, 462, 553, 685, 729
Hpy188III TCNNGA 4 cut(s) 113, 167, 362, 394
Hpy8I GTNNAC 2 cut(s) 378, 608
HpyAV CCTTC 3 cut(s) 235, 619, 734
HpyCH4III ACNGT 4 cut(s) 16, 90, 583, 712
HpyCH4V TGCA 4 cut(s) 233, 641, 745, 752
HpyF3I CTNAG 7 cut(s) 71, 122, 270, 533, 552, 739, 780
Hsp92II CATG 2 cut(s) 38, 693
HspAI GCGC 3 cut(s) 40, 68, 537
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
KspAI GTTAAC 1 cut(s) 378
Kzo9I GATC 4 cut(s) 115, 169, 402, 685
LmnI GCTCC 3 cut(s) 162, 302, 412
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 362, 483
MaeIII GTNAC 3 cut(s) 134, 524, 671
MalI GATC 4 cut(s) 117, 171, 404, 687
MboI GATC 4 cut(s) 115, 169, 402, 685
MboII GAAGA 1 cut(s) 550
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 3 cut(s) 12, 159, 299
MluCI AATT 3 cut(s) 366, 497, 516
MlyI GAGTC 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 275
MseI TTAA 2 cut(s) 377, 762
MslI CAYNNNNRTG 1 cut(s) 740
MspI CCGG 2 cut(s) 103, 276
MspR9I CCNGG 1 cut(s) 706
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 706
MvnI CGCG 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 277
NcoI CCATGG 1 cut(s) 689
NdeII GATC 4 cut(s) 115, 169, 402, 685
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 2 cut(s) 38, 693
NlaIV GGNNCC 1 cut(s) 398
NmeAIII GCCGAG 1 cut(s) 456
OliI CACNNNNGTG 1 cut(s) 740
PaeR7I CTCGAG 1 cut(s) 618
PctI GAATGC 1 cut(s) 335
PdiI GCCGGC 1 cut(s) 277
PfeI GAWTC 4 cut(s) 285, 352, 383, 730
PflMI CCANNNNNTGG 1 cut(s) 705
PfoI TCCNGGA 1 cut(s) 704
PkrI GCNGC 1 cut(s) 44
PleI GAGTC 1 cut(s) 603
PpsI GAGTC 1 cut(s) 603
Psp124BI GAGCTC 2 cut(s) 12, 299
Psp6I CCWGG 1 cut(s) 704
PspGI CCWGG 1 cut(s) 704
PspN4I GGNNCC 1 cut(s) 398
PspXI VCTCGAGB 1 cut(s) 618
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 1 cut(s) 685
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
RseI CAYNNNNRTG 1 cut(s) 740
SacI GAGCTC 2 cut(s) 12, 299
SaqAI TTAA 2 cut(s) 377, 762
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 4 cut(s) 115, 169, 402, 685
SchI GAGTC 1 cut(s) 603
ScrFI CCNGG 1 cut(s) 706
SduI GDGCHC 3 cut(s) 12, 159, 299
SfaNI GCATC 1 cut(s) 220
SfcI CTRYAG 1 cut(s) 711
Sfr274I CTCGAG 1 cut(s) 618
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 1 cut(s) 618
SmoI CTYRAG 1 cut(s) 618
Sse9I AATT 3 cut(s) 366, 497, 516
SsiI CCGC 1 cut(s) 66
SspI AATATT 1 cut(s) 663
SspMI CTAG 2 cut(s) 362, 483
SstI GAGCTC 2 cut(s) 12, 299
StyD4I CCNGG 1 cut(s) 704
StyI CCWWGG 2 cut(s) 408, 689
TaaI ACNGT 4 cut(s) 16, 90, 583, 712
TaqI TCGA 5 cut(s) 168, 228, 327, 386, 619
TasI AATT 3 cut(s) 366, 497, 516
TatI WGTACW 1 cut(s) 614
TfiI GAWTC 4 cut(s) 285, 352, 383, 730
Tru1I TTAA 2 cut(s) 377, 762
Tru9I TTAA 2 cut(s) 377, 762
TseI GCWGC 1 cut(s) 42
TspDTI ATGAA 2 cut(s) 318, 335
Van91I CCANNNNNTGG 1 cut(s) 705
XapI RAATTY 1 cut(s) 497
XbaI TCTAGA 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 618
XspI CTAG 2 cut(s) 362, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.