calcium-binding protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 521 |
| AccII | CGCG | 1 cut(s) | 452 |
| AciI | CCGC | 3 cut(s) | 226, 236, 301 |
| AclWI | GGATC | 1 cut(s) | 501 |
| AcyI | GRCGYC | 1 cut(s) | 576 |
| AfaI | GTAC | 1 cut(s) | 194 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 185 |
| AflIII | ACRYGT | 2 cut(s) | 144, 450 |
| AgsI | TTSAA | 8 cut(s) | 50, 104, 119, 158, 287, 319, 331, 559 |
| AjnI | CCWGG | 1 cut(s) | 304 |
| AluBI | AGCT | 1 cut(s) | 565 |
| AluI | AGCT | 1 cut(s) | 565 |
| AlwI | GGATC | 1 cut(s) | 501 |
| AoxI | GGCC | 2 cut(s) | 298, 402 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 367, 399 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 45, 554 |
| AspS9I | GGNCC | 2 cut(s) | 298, 380 |
| AsuHPI | GGTGA | 3 cut(s) | 41, 121, 337 |
| AsuII | TTCGAA | 1 cut(s) | 548 |
| AvaII | GGWCC | 1 cut(s) | 380 |
| BanI | GGYRCC | 1 cut(s) | 521 |
| BbsI | GAAGAC | 1 cut(s) | 174 |
| BciT130I | CCWGG | 1 cut(s) | 306 |
| BciVI | GTATCC | 1 cut(s) | 237 |
| BfaI | CTAG | 2 cut(s) | 59, 164 |
| BfuI | GTATCC | 1 cut(s) | 237 |
| BisI | GCNGC | 2 cut(s) | 236, 301 |
| BlsI | GCNGC | 2 cut(s) | 237, 302 |
| Bme1390I | CCNGG | 1 cut(s) | 306 |
| Bme18I | GGWCC | 1 cut(s) | 380 |
| BmgT120I | GGNCC | 2 cut(s) | 298, 380 |
| BmiI | GGNNCC | 3 cut(s) | 381, 395, 523 |
| BmrFI | CCNGG | 1 cut(s) | 306 |
| BmsI | GCATC | 2 cut(s) | 160, 532 |
| BoxI | GACNNNNGTC | 1 cut(s) | 174 |
| BpiI | GAAGAC | 1 cut(s) | 174 |
| BpmI | CTGGAG | 1 cut(s) | 181 |
| Bpu14I | TTCGAA | 1 cut(s) | 548 |
| Bsa29I | ATCGAT | 2 cut(s) | 366, 474 |
| BsaBI | GATNNNNATC | 1 cut(s) | 492 |
| BsaHI | GRCGYC | 1 cut(s) | 576 |
| BsaJI | CCNNGG | 1 cut(s) | 304 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 185 |
| Bse1I | ACTGG | 1 cut(s) | 198 |
| Bse8I | GATNNNNATC | 1 cut(s) | 492 |
| BseBI | CCWGG | 1 cut(s) | 306 |
| BseCI | ATCGAT | 2 cut(s) | 366, 474 |
| BseDI | CCNNGG | 1 cut(s) | 304 |
| BseGI | GGATG | 3 cut(s) | 43, 443, 493 |
| BseJI | GATNNNNATC | 1 cut(s) | 492 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 185 |
| BseNI | ACTGG | 1 cut(s) | 198 |
| Bsh1236I | CGCG | 1 cut(s) | 452 |
| BshFI | GGCC | 2 cut(s) | 300, 404 |
| BshNI | GGYRCC | 1 cut(s) | 521 |
| BshVI | ATCGAT | 2 cut(s) | 366, 474 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 185 |
| BsnI | GGCC | 2 cut(s) | 300, 404 |
| Bsp119I | TTCGAA | 1 cut(s) | 548 |
| Bsp143I | GATC | 1 cut(s) | 493 |
| BspACI | CCGC | 3 cut(s) | 226, 236, 301 |
| BspANI | GGCC | 2 cut(s) | 300, 404 |
| BspDI | ATCGAT | 2 cut(s) | 366, 474 |
| BspFNI | CGCG | 1 cut(s) | 452 |
| BspLI | GGNNCC | 3 cut(s) | 381, 395, 523 |
| BspPI | GGATC | 1 cut(s) | 501 |
| BspT104I | TTCGAA | 1 cut(s) | 548 |
| BspT107I | GGYRCC | 1 cut(s) | 521 |
| BsrI | ACTGG | 1 cut(s) | 198 |
| BssECI | CCNNGG | 1 cut(s) | 304 |
| BssMI | GATC | 1 cut(s) | 493 |
| BssNI | GRCGYC | 1 cut(s) | 576 |
| Bst2UI | CCWGG | 1 cut(s) | 306 |
| Bst4CI | ACNGT | 2 cut(s) | 79, 425 |
| BstACI | GRCGYC | 1 cut(s) | 576 |
| BstBI | TTCGAA | 1 cut(s) | 548 |
| BstC8I | GCNNGC | 2 cut(s) | 139, 274 |
| BstF5I | GGATG | 3 cut(s) | 43, 443, 493 |
| BstFNI | CGCG | 1 cut(s) | 452 |
| BstKTI | GATC | 1 cut(s) | 496 |
| BstMBI | GATC | 1 cut(s) | 493 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 309, 542 |
| BstNI | CCWGG | 1 cut(s) | 306 |
| BstNSI | RCATGY | 2 cut(s) | 148, 585 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 174 |
| BstSCI | CCNGG | 1 cut(s) | 304 |
| BstUI | CGCG | 1 cut(s) | 452 |
| BstV2I | GAAGAC | 1 cut(s) | 174 |
| BstX2I | RGATCY | 1 cut(s) | 493 |
| BstYI | RGATCY | 1 cut(s) | 493 |
| Bsu15I | ATCGAT | 2 cut(s) | 366, 474 |
| BsuI | GTATCC | 1 cut(s) | 237 |
| BsuRI | GGCC | 2 cut(s) | 300, 404 |
| BsuTUI | ATCGAT | 2 cut(s) | 366, 474 |
| BtsCI | GGATG | 3 cut(s) | 43, 443, 493 |
| BtsI | GCAGTG | 2 cut(s) | 19, 419 |
| BtsIMutI | CAGTG | 2 cut(s) | 19, 419 |
| Cac8I | GCNNGC | 2 cut(s) | 139, 274 |
| Cfr13I | GGNCC | 2 cut(s) | 298, 380 |
| ClaI | ATCGAT | 2 cut(s) | 366, 474 |
| CseI | GACGC | 1 cut(s) | 584 |
| Csp6I | GTAC | 1 cut(s) | 193 |
| CviAII | CATG | 3 cut(s) | 145, 572, 582 |
| CviJI | RGCY | 6 cut(s) | 189, 300, 339, 362, 404, 565 |
| CviKI_1 | RGCY | 6 cut(s) | 189, 300, 339, 362, 404, 565 |
| CviQI | GTAC | 1 cut(s) | 193 |
| DpnI | GATC | 1 cut(s) | 495 |
| DpnII | GATC | 1 cut(s) | 493 |
| EciI | GGCGGA | 1 cut(s) | 215 |
| Eco47I | GGWCC | 1 cut(s) | 380 |
| EcoO109I | RGGNCCY | 1 cut(s) | 380 |
| EcoRII | CCWGG | 1 cut(s) | 304 |
| FaeI | CATG | 3 cut(s) | 148, 575, 585 |
| FatI | CATG | 3 cut(s) | 144, 571, 581 |
| Fnu4HI | GCNGC | 2 cut(s) | 236, 301 |
| FokI | GGATG | 3 cut(s) | 50, 430, 500 |
| Fsp4HI | GCNGC | 2 cut(s) | 236, 301 |
| FspBI | CTAG | 2 cut(s) | 59, 164 |
| GluI | GCNGC | 2 cut(s) | 236, 301 |
| GsuI | CTGGAG | 1 cut(s) | 181 |
| HaeIII | GGCC | 2 cut(s) | 300, 404 |
| HgaI | GACGC | 1 cut(s) | 584 |
| Hin1I | GRCGYC | 1 cut(s) | 576 |
| Hin1II | CATG | 3 cut(s) | 148, 575, 585 |
| HinfI | GANTC | 1 cut(s) | 126 |
| HphI | GGTGA | 3 cut(s) | 41, 121, 337 |
| Hpy188I | TCNGA | 1 cut(s) | 268 |
| Hpy188III | TCNNGA | 2 cut(s) | 59, 391 |
| HpyAV | CCTTC | 2 cut(s) | 110, 415 |
| HpyCH4III | ACNGT | 2 cut(s) | 79, 425 |
| HpyCH4V | TGCA | 3 cut(s) | 151, 312, 585 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 309, 542 |
| Hsp92I | GRCGYC | 1 cut(s) | 576 |
| Hsp92II | CATG | 3 cut(s) | 148, 575, 585 |
| Kzo9I | GATC | 1 cut(s) | 493 |
| LmnI | GCTCC | 1 cut(s) | 228 |
| LpnPI | CCDG | 6 cut(s) | 211, 219, 258, 291, 318, 376 |
| LweI | GCATC | 2 cut(s) | 160, 532 |
| MaeI | CTAG | 2 cut(s) | 59, 164 |
| MalI | GATC | 1 cut(s) | 495 |
| MboI | GATC | 1 cut(s) | 493 |
| MboII | GAAGA | 4 cut(s) | 146, 179, 488, 547 |
| MflI | RGATCY | 1 cut(s) | 493 |
| MluI | ACGCGT | 1 cut(s) | 450 |
| MmeI | TCCRAC | 1 cut(s) | 61 |
| MnlI | CCTC | 7 cut(s) | 62, 189, 234, 262, 363, 366, 479 |
| MroXI | GAANNNNTTC | 2 cut(s) | 45, 554 |
| MseI | TTAA | 2 cut(s) | 216, 279 |
| MspR9I | CCNGG | 1 cut(s) | 306 |
| MvaI | CCWGG | 1 cut(s) | 306 |
| MvnI | CGCG | 1 cut(s) | 452 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 309, 542 |
| NdeII | GATC | 1 cut(s) | 493 |
| NlaIII | CATG | 3 cut(s) | 148, 575, 585 |
| NlaIV | GGNNCC | 3 cut(s) | 381, 395, 523 |
| NspI | RCATGY | 2 cut(s) | 148, 585 |
| NspV | TTCGAA | 1 cut(s) | 548 |
| PciI | ACATGT | 1 cut(s) | 144 |
| PdmI | GAANNNNTTC | 2 cut(s) | 45, 554 |
| PfeI | GAWTC | 1 cut(s) | 126 |
| PkrI | GCNGC | 2 cut(s) | 237, 302 |
| PpuMI | RGGWCCY | 1 cut(s) | 380 |
| PscI | ACATGT | 1 cut(s) | 144 |
| PshAI | GACNNNNGTC | 1 cut(s) | 174 |
| Psp5II | RGGWCCY | 1 cut(s) | 380 |
| Psp6I | CCWGG | 1 cut(s) | 304 |
| PspGI | CCWGG | 1 cut(s) | 304 |
| PspN4I | GGNNCC | 3 cut(s) | 381, 395, 523 |
| PspPI | GGNCC | 2 cut(s) | 298, 380 |
| PspPPI | RGGWCCY | 1 cut(s) | 380 |
| PsuI | RGATCY | 1 cut(s) | 493 |
| RsaI | GTAC | 1 cut(s) | 194 |
| RsaNI | GTAC | 1 cut(s) | 193 |
| SaqAI | TTAA | 2 cut(s) | 216, 279 |
| SatI | GCNGC | 2 cut(s) | 236, 301 |
| Sau3AI | GATC | 1 cut(s) | 493 |
| Sau96I | GGNCC | 2 cut(s) | 298, 380 |
| ScrFI | CCNGG | 1 cut(s) | 306 |
| SfaNI | GCATC | 2 cut(s) | 160, 532 |
| SfuI | TTCGAA | 1 cut(s) | 548 |
| SinI | GGWCC | 1 cut(s) | 380 |
| SsiI | CCGC | 3 cut(s) | 226, 236, 301 |
| SspMI | CTAG | 2 cut(s) | 59, 164 |
| StyD4I | CCNGG | 1 cut(s) | 304 |
| TaaI | ACNGT | 2 cut(s) | 79, 425 |
| TaqI | TCGA | 4 cut(s) | 366, 408, 474, 548 |
| TatI | WGTACW | 1 cut(s) | 192 |
| TauI | GCSGC | 2 cut(s) | 238, 303 |
| TfiI | GAWTC | 1 cut(s) | 126 |
| Tru1I | TTAA | 2 cut(s) | 216, 279 |
| Tru9I | TTAA | 2 cut(s) | 216, 279 |
| TscAI | CASTG | 2 cut(s) | 19, 419 |
| TspDTI | ATGAA | 2 cut(s) | 44, 54 |
| TspRI | CASTG | 2 cut(s) | 19, 419 |
| VpaK11BI | GGWCC | 1 cut(s) | 380 |
| XbaI | TCTAGA | 1 cut(s) | 58 |
| XceI | RCATGY | 2 cut(s) | 148, 585 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 355 |
| XmnI | GAANNNNTTC | 2 cut(s) | 45, 554 |
| XspI | CTAG | 2 cut(s) | 59, 164 |