RchiOBHm_Chr7g0185221

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000188
Physical Location & Seq
Reverse (-)
5423257 .. 5424577
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16528

Sequence Viewer

Length: 1254 bp
ATGACGGAATTGACCGTAGTCATGGAAGATCATGGCAGTACAAATATTGATGTTGAAGCATTGGAACAGAGGATGAAAGCAAAGCTTCGCAGTGATTCACCCTTGTCTGCTCAGTGCTGCATTTTCAGAGTTCCTGAATTACTCCGGAAACATAAACCAGAGGCATCTGATGTTGTCTCAATCGGACCCTTTCATCACAGTAGCAGCAAACTTTTTGCAAACATGAAAGATGTGAAACAGTGGTATTTAAATTCTCTACTCTTAAGAAAGAATATAAGTTTAAAAGCTTTGATCCAAGGTATTGATAGTATTACTTGGTTTGAGAAACGCGCCCGTGATTATTATGCAGAATCATTTAATCATCTTGACCAGAACCAATTCATAGAAATGATGATAATTGATGGCTGCTTCCTATTAGAACTATTTCAGAGACGTTCCAATATTTTCCCAGATTCAGATAAGGTCAATGTACTCAATGATCCCTTATCCAACATGGGTTGCATGATCCAGTATCTTTGCCATGATCTTCTGCTGCTAGAAAATCAACTGCCTTGGTTTGTTCTCGAGTCTCTATATAACCTTACCTGCTACGAACGAGGCTCTCTCACTAGGCTGGTTCTCAACTTCTTCATCAGCGGACTATCGTCACTGGCTCATAATTGCAACGGTTATCGCAATCACAGTCATGATAATGAAATTCTACACATACTTGATCTGATAAGAAGTGCTATTGTTGTTCCACTCCACAAATTTGAATCAATCGAACAAGAGATTACACTGCCCCATGCAACTGAACTCTTTGAGGCAGGCATTCAATTCAAAAGAAGCTTAGTTGATATTAATGAACCTTTGTTCAGGAACCGCATAGCCTTCGAGCAATGCTATCATGGTCGAGACTATAAAGTAACTTCTTATGCCTCTTTAATGGATAACCTCATTGCTTCCAGCAAGGATGTTGTTTTTCTCTCTGACAATGAAATAATCCGTAATTGGTTGAGTGCTGAAGATGCTTCCCAGGTGTTCAGTAAGCTTGTTAATGACACATTGATTGTTGATTTTTGCTATGGCAGGCTCTGTGCTGCAGTGAATAAATATTACAAAGTTAGATGGAACCAGTGGCTAGCCCGACTGAAGCGTGATTACTTATCTAATCCATGGAAAATCACTTCTTTGGTTGCAGCCTTTATCCTTCTGGTTCTCACCCTATTGCAGACAACATATACCATTCAGCAATACTATTCTCCTCCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

417

Amino Acids

48.4

Weight (kDa)

6.36

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 41 - 278 6.4e-50 Plant protein of unknown function
DUF247 PF03140 279 - 400 3.8e-36 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000188)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02500 FvH4_4g03110 FvH4_5g11900 FvH4_5g11910 FvH4_5g19510 FvH4_6g53141 FvH4_6g53141 FvH4_6g53143 FvH4_6g53143 FvH4_6g53260 FvH4_6g53260 FvH4_6g53260 FvH4_6g53280
malus_domestica MD00G1110800.v1.1 MD00G1111300.v1.1 MD08G1225300.v1.1 MD08G1225500.v1.1 MD08G1225700.v1.1 MD09G1013500.v1.1 MD09G1013700.v1.1 MD09G1013800.v1.1 MD09G1014000.v1.1
prunus_persica Prupe.1G028300_v2.0.a1 Prupe.1G029300_v2.0.a1 Prupe.1G029400_v2.0.a1 Prupe.1G029800_v2.0.a1 Prupe.1G165900_v2.0.a1 Prupe.3G303500_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029200_v2.0.a1 Prupe.7G037400_v2.0.a1
pyrus_communis pycom08g19660 pycom08g19690 pycom08g19710 pycom08g19720 pycom111g01160 pycom111g01170 pycom17g01140
rosa_chinensis RchiOBHm_Chr2g0111831 RchiOBHm_Chr2g0138211 RchiOBHm_Chr2g0163091 RchiOBHm_Chr2g0175051 RchiOBHm_Chr2g0175141 RchiOBHm_Chr2g0175171 RchiOBHm_Chr2g0175191 RchiOBHm_Chr3g0483151 RchiOBHm_Chr3g0483161 RchiOBHm_Chr3g0483171 RchiOBHm_Chr4g0389941 RchiOBHm_Chr4g0411021 RchiOBHm_Chr4g0411031 RchiOBHm_Chr4g0411041 RchiOBHm_Chr5g0041861 RchiOBHm_Chr5g0058261 RchiOBHm_Chr5g0065661 RchiOBHm_Chr5g0070431 RchiOBHm_Chr7g0185221 RchiOBHm_Chr7g0185581 RchiOBHm_Chr7g0185591 RchiOBHm_Chr7g0185611 RchiOBHm_Chr7g0185641
rosa_laevigata RLG00000004895 RLG00000004896 RLG00000004897 RLG00000008379 RLG00000021370 RLG00000022309 RLG00000034099 RLG00000035811 RLG00000036132 RLG00000036450
rosa_multiflora Rmu_co8229993.1_g000001 Rmu_co8360165.1_g000001 Rmu_co8461695.1_g000001 Rmu_sc0000213.1_g000004 Rmu_sc0000235.1_g000066 Rmu_sc0000367.1_g000060 Rmu_sc0000861.1_g000057 Rmu_sc0000861.1_g000068 Rmu_sc0001244.1_g000011 Rmu_sc0001244.1_g000013 Rmu_sc0001323.1_g000018 Rmu_sc0002180.1_g000032 Rmu_sc0002280.1_g000009 Rmu_sc0003226.1_g000095 Rmu_sc0003226.1_g000096 Rmu_sc0003555.1_g000010 Rmu_sc0003555.1_g000018 Rmu_sc0003920.1_g000011 Rmu_sc0003984.1_g000012 Rmu_sc0004336.1_g000026 Rmu_sc0006027.1_g000005 Rmu_sc0006570.1_g000004 Rmu_sc0007961.1_g000010 Rmu_sc0008747.1_g000001 Rmu_sc0008834.1_g000005 Rmu_sc0010190.1_g000004 Rmu_sc0010190.1_g000007 Rmu_sc0010190.1_g000008 Rmu_sc0010642.1_g000012 Rmu_sc0011497.1_g000002
rosa_roxburghii Rroxscaffold_1G00010770 Rroxscaffold_1G00010780 Rroxscaffold_1G00011860 Rroxscaffold_1G00015470 Rroxscaffold_1G00038670 Rroxscaffold_2G00077520 Rroxscaffold_2G00077550 Rroxscaffold_2G00106420 Rroxscaffold_3G00268750 Rroxscaffold_3G00268770 Rroxscaffold_3G00268780 Rroxscaffold_3G00268790 Rroxscaffold_3G00269130 Rroxscaffold_5G00336020 Rroxscaffold_5G00343150 Rroxscaffold_5G00355700
rosa_rugosa Rorug02G0500300 Rorug02G0581200 Rorug03G0204700 Rorug03G0327300 Rorug05G0196100 Rorug05G0196400 Rorug05G0405900 Rorug05G0406000 Rorug06G0467000 Rorug06G0467000 Rorug06G0470200 Rorug06G0470200 Rorug07G0180500
rosa_samantha Rh2AG566700 Rh2AG661900 Rh2AG663200 Rh3CG286400 Rh3CG286500 Rh3CG366100 Rh4DG024200 Rh4DG076600 Rh4DG160000 Rh4DG160100 Rh5BG286800 Rh5DG295500 Rh5DG461000 Rh7DG072100 Rh7DG075500
rosa_wichuraiana Rw1G002310 Rw1G015820 Rw2G046920 Rw2G054280 Rw3G022880 Rw3G029170 Rw4G002330 Rw4G013670 Rw5G026420 Rw5G026440 Rw5G040490 Rw5G042130 Rw7G006300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 593
AccII CGCG 1 cut(s) 330
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 2 cut(s) 636, 862
AclWI GGATC 3 cut(s) 286, 473, 499
AcsI RAATTY 3 cut(s) 250, 696, 749
AcuI CTGAAG 2 cut(s) 1023, 1151
AfaI GTAC 2 cut(s) 40, 471
AflII CTTAAG 1 cut(s) 262
AgsI TTSAA 4 cut(s) 56, 755, 815, 820
AjnI CCWGG 1 cut(s) 1014
AluBI AGCT 4 cut(s) 85, 287, 828, 1030
AluI AGCT 4 cut(s) 85, 287, 828, 1030
Alw26I GTCTC 4 cut(s) 181, 424, 573, 888
AlwI GGATC 3 cut(s) 286, 473, 499
AlwNI CAGNNNCTG 1 cut(s) 1074
Ama87I CYCGRG 1 cut(s) 563
Aor13HI TCCGGA 1 cut(s) 144
ApeKI GCWGC 6 cut(s) 117, 204, 405, 532, 1079, 1178
ApoI RAATTY 3 cut(s) 250, 696, 749
AseI ATTAAT 1 cut(s) 840
Asp700I GAANNNNTTC 2 cut(s) 377, 423
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 90, 1192
AsuNHI GCTAGC 1 cut(s) 1120
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 1124, 1156
BbvI GCAGC 6 cut(s) 104, 216, 392, 519, 1066, 1190
BccI CCATC 2 cut(s) 395, 1101
BcgI CGANNNNNNTGC 2 cut(s) 853, 887
BciT130I CCWGG 1 cut(s) 1016
BcoDI GTCTC 4 cut(s) 181, 424, 573, 888
BfaI CTAG 3 cut(s) 536, 609, 1121
BfmI CTRYAG 1 cut(s) 1080
BfrI CTTAAG 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 593
BisI GCNGC 6 cut(s) 118, 205, 406, 533, 1080, 1179
BlsI GCNGC 6 cut(s) 119, 206, 407, 534, 1081, 1180
Bme1390I CCNGG 1 cut(s) 1016
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 3 cut(s) 187, 860, 1112
BmrFI CCNGG 1 cut(s) 1016
BmsI GCATC 2 cut(s) 173, 997
BmtI GCTAGC 1 cut(s) 1124
BoxI GACNNNNGTC 2 cut(s) 17, 643
BplI GAGNNNNNCTC 2 cut(s) 588, 620
BsaJI CCNNGG 4 cut(s) 295, 551, 1014, 1154
BsaWI WCCGGW 1 cut(s) 144
Bse1I ACTGG 3 cut(s) 508, 654, 1114
Bse3DI GCAATG 2 cut(s) 884, 936
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 1 cut(s) 1016
BseDI CCNNGG 4 cut(s) 295, 551, 1014, 1154
BseGI GGATG 2 cut(s) 78, 958
BseMI GCAATG 2 cut(s) 884, 936
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 3 cut(s) 508, 654, 1114
BseRI GAGGAG 1 cut(s) 1233
BseXI GCAGC 6 cut(s) 104, 216, 392, 519, 1066, 1190
Bsh1236I CGCG 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 1 cut(s) 145
BsmAI GTCTC 4 cut(s) 181, 424, 573, 888
BsmBI CGTCTC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 810
BsoBI CYCGRG 1 cut(s) 563
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 6 cut(s) 28, 291, 478, 504, 523, 712
Bsp19I CCATGG 1 cut(s) 1154
BspACI CCGC 2 cut(s) 636, 862
BspCNI CTCAG 1 cut(s) 124
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 3 cut(s) 187, 860, 1112
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 1 cut(s) 593
BspOI GCTAGC 1 cut(s) 1124
BspPI GGATC 3 cut(s) 286, 473, 499
BspTI CTTAAG 1 cut(s) 262
BsrDI GCAATG 2 cut(s) 884, 936
BsrI ACTGG 3 cut(s) 508, 654, 1114
BssECI CCNNGG 4 cut(s) 295, 551, 1014, 1154
BssMI GATC 6 cut(s) 28, 291, 478, 504, 523, 712
BssT1I CCWWGG 3 cut(s) 295, 551, 1154
Bst2UI CCWGG 1 cut(s) 1016
Bst4CI ACNGT 5 cut(s) 16, 200, 240, 668, 683
BstAFI CTTAAG 1 cut(s) 262
BstC8I GCNNGC 3 cut(s) 808, 1070, 1122
BstDEI CTNAG 3 cut(s) 111, 829, 1248
BstDSI CCRYGG 1 cut(s) 1154
BstF5I GGATG 2 cut(s) 78, 958
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 6 cut(s) 31, 294, 481, 507, 526, 715
BstMAI GTCTC 4 cut(s) 181, 424, 573, 888
BstMBI GATC 6 cut(s) 28, 291, 478, 504, 523, 712
BstMWI GCNNNNNNNGC 1 cut(s) 1007
BstNI CCWGG 1 cut(s) 1016
BstPAI GACNNNNGTC 2 cut(s) 17, 643
BstSCI CCNGG 1 cut(s) 1014
BstSFI CTRYAG 1 cut(s) 1080
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 6 cut(s) 104, 216, 392, 519, 1066, 1190
BtgI CCRYGG 1 cut(s) 1154
BtsCI GGATG 2 cut(s) 78, 958
BtsI GCAGTG 3 cut(s) 97, 776, 1089
BtsIMutI CAGTG 7 cut(s) 97, 119, 245, 647, 776, 1089, 1121
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 808, 1070, 1122
CaiI CAGNNNCTG 1 cut(s) 1074
CciI TCATGA 1 cut(s) 685
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 39, 470
CviQI GTAC 2 cut(s) 39, 470
DdeI CTNAG 3 cut(s) 111, 829, 1248
DpnI GATC 6 cut(s) 30, 293, 480, 506, 525, 714
DpnII GATC 6 cut(s) 28, 291, 478, 504, 523, 712
DraI TTTAAA 2 cut(s) 249, 282
Eco130I CCWWGG 3 cut(s) 295, 551, 1154
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 1023, 1151
Eco88I CYCGRG 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 1014
EcoT14I CCWWGG 3 cut(s) 295, 551, 1154
ErhI CCWWGG 3 cut(s) 295, 551, 1154
Esp3I CGTCTC 1 cut(s) 424
FalI AAGNNNNNCTT 2 cut(s) 69, 101
Fnu4HI GCNGC 6 cut(s) 118, 205, 406, 533, 1080, 1179
FokI GGATG 2 cut(s) 85, 965
Fsp4HI GCNGC 6 cut(s) 118, 205, 406, 533, 1080, 1179
FspBI CTAG 3 cut(s) 536, 609, 1121
GlaI GCGC 1 cut(s) 331
GluI GCNGC 6 cut(s) 118, 205, 406, 533, 1080, 1179
HapII CCGG 1 cut(s) 145
HhaI GCGC 1 cut(s) 332
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HindIII AAGCTT 4 cut(s) 83, 285, 826, 1028
HinfI GANTC 5 cut(s) 95, 350, 452, 566, 755
HpaII CCGG 1 cut(s) 145
HphI GGTGA 2 cut(s) 90, 1192
Hpy188I TCNGA 7 cut(s) 128, 169, 185, 429, 457, 717, 970
Hpy188III TCNNGA 7 cut(s) 134, 145, 365, 563, 686, 856, 893
HpyAV CCTTC 2 cut(s) 880, 1199
HpyCH4III ACNGT 5 cut(s) 16, 200, 240, 668, 683
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 9 cut(s) 120, 218, 347, 501, 663, 788, 1082, 1178, 1210
HpyF10VI GCNNNNNNNGC 1 cut(s) 1007
HpyF3I CTNAG 3 cut(s) 111, 829, 1248
HpySE526I ACGT 1 cut(s) 433
HspAI GCGC 1 cut(s) 330
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 6 cut(s) 28, 291, 478, 504, 523, 712
Lsp1109I GCAGC 6 cut(s) 104, 216, 392, 519, 1066, 1190
LweI GCATC 2 cut(s) 173, 997
MaeI CTAG 3 cut(s) 536, 609, 1121
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 2 cut(s) 645, 904
MalI GATC 6 cut(s) 30, 293, 480, 506, 525, 714
MboI GATC 6 cut(s) 28, 291, 478, 504, 523, 712
MboII GAAGA 4 cut(s) 38, 518, 619, 1016
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 7 cut(s) 63, 154, 590, 796, 928, 944, 1254
MroI TCCGGA 1 cut(s) 144
MroXI GAANNNNTTC 2 cut(s) 377, 423
MseI TTAA 7 cut(s) 248, 263, 281, 357, 840, 923, 1035
MslI CAYNNNNRTG 3 cut(s) 386, 684, 690
MspA1I CMGCKG 1 cut(s) 636
MspCI CTTAAG 1 cut(s) 262
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 1 cut(s) 1016
Mva1269I GAATGC 1 cut(s) 810
MvaI CCWGG 1 cut(s) 1016
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 1007
NcoI CCATGG 1 cut(s) 1154
NdeII GATC 6 cut(s) 28, 291, 478, 504, 523, 712
NheI GCTAGC 1 cut(s) 1120
NlaIV GGNNCC 3 cut(s) 187, 860, 1112
NmuCI GTSAC 1 cut(s) 645
PaeR7I CTCGAG 1 cut(s) 563
PagI TCATGA 1 cut(s) 685
PctI GAATGC 1 cut(s) 810
PdmI GAANNNNTTC 2 cut(s) 377, 423
PfeI GAWTC 4 cut(s) 95, 350, 452, 755
PkrI GCNGC 6 cut(s) 119, 206, 407, 534, 1081, 1180
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PshAI GACNNNNGTC 2 cut(s) 17, 643
PshBI ATTAAT 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 1014
PspGI CCWGG 1 cut(s) 1014
PspN4I GGNNCC 3 cut(s) 187, 860, 1112
PspPI GGNCC 1 cut(s) 185
PstI CTGCAG 1 cut(s) 1084
PstNI CAGNNNCTG 1 cut(s) 1074
RsaI GTAC 2 cut(s) 40, 471
RsaNI GTAC 2 cut(s) 39, 470
RseI CAYNNNNRTG 3 cut(s) 386, 684, 690
SaqAI TTAA 7 cut(s) 248, 263, 281, 357, 840, 923, 1035
SatI GCNGC 6 cut(s) 118, 205, 406, 533, 1080, 1179
Sau3AI GATC 6 cut(s) 28, 291, 478, 504, 523, 712
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 1016
SfaNI GCATC 2 cut(s) 173, 997
SfcI CTRYAG 1 cut(s) 1080
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 563
SmiI ATTTAAAT 1 cut(s) 249
SmiMI CAYNNNNRTG 3 cut(s) 386, 684, 690
SmlI CTYRAG 2 cut(s) 262, 563
SmoI CTYRAG 2 cut(s) 262, 563
SsiI CCGC 2 cut(s) 636, 862
SspI AATATT 3 cut(s) 46, 442, 1094
SspMI CTAG 3 cut(s) 536, 609, 1121
StyD4I CCNGG 1 cut(s) 1014
StyI CCWWGG 3 cut(s) 295, 551, 1154
SwaI ATTTAAAT 1 cut(s) 249
TaaI ACNGT 5 cut(s) 16, 200, 240, 668, 683
TaiI ACGT 1 cut(s) 436
TaqI TCGA 4 cut(s) 564, 762, 873, 892
TatI WGTACW 2 cut(s) 38, 469
TfiI GAWTC 4 cut(s) 95, 350, 452, 755
Tru1I TTAA 7 cut(s) 248, 263, 281, 357, 840, 923, 1035
Tru9I TTAA 7 cut(s) 248, 263, 281, 357, 840, 923, 1035
TscAI CASTG 7 cut(s) 97, 119, 245, 654, 783, 1089, 1121
TseFI GTSAC 1 cut(s) 645
TseI GCWGC 6 cut(s) 117, 204, 405, 532, 1079, 1178
Tsp45I GTSAC 1 cut(s) 645
TspDTI ATGAA 8 cut(s) 89, 182, 239, 370, 619, 708, 858, 990
TspGWI ACGGA 2 cut(s) 20, 974
TspRI CASTG 7 cut(s) 97, 119, 245, 654, 783, 1089, 1121
Vha464I CTTAAG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 185
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 3 cut(s) 250, 696, 749
XhoI CTCGAG 1 cut(s) 563
XmnI GAANNNNTTC 2 cut(s) 377, 423
XspI CTAG 3 cut(s) 536, 609, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.