Rw1G015820

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000188
Physical Location & Seq
Reverse (-)
35239721 .. 35241097
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015820.1

Sequence Viewer

Length: 1377 bp
ATGGCAAATATTATGGGAAAAAGTAGTGCAGGACCATCTACTATTATAAACGACAGATCGAAGTTGATTGATGCATTGGAAAACACCATTACGGACAAGCTTCCCCATGATTCACCCTTTTCTGATACAAGCTGCATCTTCAGAGTTCCTCAACTAATCCGGCGACATAACCCGGAGGCGTATCAACCCCAGGTTGCCTCAATAGGACCCATCCATTATGGAGGTGAGAAATTTAAACCCATGGAAACTGTGAAGCGGTGGTATTTAAAGAACCTTCTCATCTCAAACTCGAATATTACTTTGAGAAAGTTGATTGAAGATGTTACTGAGTTTGAGAAACGCGCCCGTCTTTTCTATGCTGACCCCCTTGATCATATTAGCGAGAGTGACTTTATAGAAATGATGATACTAGACGGTTGCTTCATAACAGAATTATTTCGGAAGTCATTCTTTGAGGACAAGAAGATTACTGACCCCGTATTCAACATGATTTGCAAATTCCAACATATATGCAATGACCTTTTGCTTCTAGAAAATCAGCTACCTTGGTTTGTTCTCGAGAGTTTATATGCTCTTACAGAGGGCTTGTACCCAAGTCGGCACCGCCTTGACGTACTGATGCTCACCGTCTTTACCTCACAAGAACCATTGCAACATAACTGCTCATCATATGCAAGTTATCTTCTCGCCAATCCTAACACTTGTTATGAAGCTTTGCACATACTTGATCTGGTAAGAAATTCCATAGTTTTTTCTTTCCGAAAAGTTTCACCTACCAATAACAGAAGCAGAAATTTTTTTGGGATAGTTAAAAAACAAGAAAACGAGTTTGAGTCAGAAATACCTTTTGCAACTACTCTTTCAGAGGCCGGCGTTAAATTCAAAAGAGGTATTGGCAGTGAAAGCATATTGAACATAGAATTCAGAGATCGGGTTTTAACTATTCCACCTCTCGAGATCGGAGAGTTGACCGAACCATTGTTCAGAAATCTTGTTGCCTTTGAGCAGTGCTACCATGGTCGTTCAGATGAAATTACATCTTATGCCGCTTTGATGAAAAAACTCCTTGGTTCCAGCAAGGATATGCATTTTCTTCGTGAGGCAAAAATAATTGGGAACTGGCTGAGCGATGAGGATGGCTTTCAGTTCTTCAACAAGCTTTACAATGACACTGTACTCAATAGGTACTTCTATGAGCACCTCTGCCGTGAAGTGAATGGTTATTACGAAGTTACTTGGAACAAGTGGCTGGCAGATTTCAAGCGTGAGCATTTAGCTGACCCATGGAAAGTTATTTCCTTCTGTGTAGGAATTATCCTTCTCGTTATCACTGGACTGCAAGCTGCATATAGCATTTTCAAATGGTGGTTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

458

Amino Acids

53.24

Weight (kDa)

6.21

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 46 - 442 4.4e-107 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000188)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02500 FvH4_4g03110 FvH4_5g11900 FvH4_5g11910 FvH4_5g19510 FvH4_6g53141 FvH4_6g53141 FvH4_6g53143 FvH4_6g53143 FvH4_6g53260 FvH4_6g53260 FvH4_6g53260 FvH4_6g53280
malus_domestica MD00G1110800.v1.1 MD00G1111300.v1.1 MD08G1225300.v1.1 MD08G1225500.v1.1 MD08G1225700.v1.1 MD09G1013500.v1.1 MD09G1013700.v1.1 MD09G1013800.v1.1 MD09G1014000.v1.1
prunus_persica Prupe.1G028300_v2.0.a1 Prupe.1G029300_v2.0.a1 Prupe.1G029400_v2.0.a1 Prupe.1G029800_v2.0.a1 Prupe.1G165900_v2.0.a1 Prupe.3G303500_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029200_v2.0.a1 Prupe.7G037400_v2.0.a1
pyrus_communis pycom08g19660 pycom08g19690 pycom08g19710 pycom08g19720 pycom111g01160 pycom111g01170 pycom17g01140
rosa_chinensis RchiOBHm_Chr2g0111831 RchiOBHm_Chr2g0138211 RchiOBHm_Chr2g0163091 RchiOBHm_Chr2g0175051 RchiOBHm_Chr2g0175141 RchiOBHm_Chr2g0175171 RchiOBHm_Chr2g0175191 RchiOBHm_Chr3g0483151 RchiOBHm_Chr3g0483161 RchiOBHm_Chr3g0483171 RchiOBHm_Chr4g0389941 RchiOBHm_Chr4g0411021 RchiOBHm_Chr4g0411031 RchiOBHm_Chr4g0411041 RchiOBHm_Chr5g0041861 RchiOBHm_Chr5g0058261 RchiOBHm_Chr5g0065661 RchiOBHm_Chr5g0070431 RchiOBHm_Chr7g0185221 RchiOBHm_Chr7g0185581 RchiOBHm_Chr7g0185591 RchiOBHm_Chr7g0185611 RchiOBHm_Chr7g0185641
rosa_laevigata RLG00000004895 RLG00000004896 RLG00000004897 RLG00000008379 RLG00000021370 RLG00000022309 RLG00000034099 RLG00000035811 RLG00000036132 RLG00000036450
rosa_multiflora Rmu_co8229993.1_g000001 Rmu_co8360165.1_g000001 Rmu_co8461695.1_g000001 Rmu_sc0000213.1_g000004 Rmu_sc0000235.1_g000066 Rmu_sc0000367.1_g000060 Rmu_sc0000861.1_g000057 Rmu_sc0000861.1_g000068 Rmu_sc0001244.1_g000011 Rmu_sc0001244.1_g000013 Rmu_sc0001323.1_g000018 Rmu_sc0002180.1_g000032 Rmu_sc0002280.1_g000009 Rmu_sc0003226.1_g000095 Rmu_sc0003226.1_g000096 Rmu_sc0003555.1_g000010 Rmu_sc0003555.1_g000018 Rmu_sc0003920.1_g000011 Rmu_sc0003984.1_g000012 Rmu_sc0004336.1_g000026 Rmu_sc0006027.1_g000005 Rmu_sc0006570.1_g000004 Rmu_sc0007961.1_g000010 Rmu_sc0008747.1_g000001 Rmu_sc0008834.1_g000005 Rmu_sc0010190.1_g000004 Rmu_sc0010190.1_g000007 Rmu_sc0010190.1_g000008 Rmu_sc0010642.1_g000012 Rmu_sc0011497.1_g000002
rosa_roxburghii Rroxscaffold_1G00010770 Rroxscaffold_1G00010780 Rroxscaffold_1G00011860 Rroxscaffold_1G00015470 Rroxscaffold_1G00038670 Rroxscaffold_2G00077520 Rroxscaffold_2G00077550 Rroxscaffold_2G00106420 Rroxscaffold_3G00268750 Rroxscaffold_3G00268770 Rroxscaffold_3G00268780 Rroxscaffold_3G00268790 Rroxscaffold_3G00269130 Rroxscaffold_5G00336020 Rroxscaffold_5G00343150 Rroxscaffold_5G00355700
rosa_rugosa Rorug02G0500300 Rorug02G0581200 Rorug03G0204700 Rorug03G0327300 Rorug05G0196100 Rorug05G0196400 Rorug05G0405900 Rorug05G0406000 Rorug06G0467000 Rorug06G0467000 Rorug06G0470200 Rorug06G0470200 Rorug07G0180500
rosa_samantha Rh2AG566700 Rh2AG661900 Rh2AG663200 Rh3CG286400 Rh3CG286500 Rh3CG366100 Rh4DG024200 Rh4DG076600 Rh4DG160000 Rh4DG160100 Rh5BG286800 Rh5DG295500 Rh5DG461000 Rh7DG072100 Rh7DG075500
rosa_wichuraiana Rw1G002310 Rw1G015820 Rw2G046920 Rw2G054280 Rw3G022880 Rw3G029170 Rw4G002330 Rw4G013670 Rw5G026420 Rw5G026440 Rw5G040490 Rw5G042130 Rw7G006300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 600
AccII CGCG 1 cut(s) 342
AciI CCGC 3 cut(s) 256, 604, 1047
AcsI RAATTY 6 cut(s) 230, 497, 739, 793, 878, 920
AcuI CTGAAG 1 cut(s) 124
AfaI GTAC 4 cut(s) 590, 615, 1176, 1187
AgsI TTSAA 7 cut(s) 317, 484, 883, 913, 1153, 1261, 1360
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 7 cut(s) 100, 132, 541, 713, 1159, 1277, 1343
AluI AGCT 7 cut(s) 100, 132, 541, 713, 1159, 1277, 1343
Alw21I GWGCWC 1 cut(s) 1200
Ama87I CYCGRG 2 cut(s) 557, 953
AoxI GGCC 1 cut(s) 867
ApeKI GCWGC 2 cut(s) 132, 1343
ApoI RAATTY 6 cut(s) 230, 497, 739, 793, 878, 920
Asp700I GAANNNNTTC 3 cut(s) 435, 446, 766
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 32, 206
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 4 cut(s) 105, 236, 616, 762
AvaI CYCGRG 2 cut(s) 557, 953
AvaII GGWCC 2 cut(s) 32, 206
BanI GGYRCC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 1200
BbvI GCAGC 2 cut(s) 119, 1330
BccI CCATC 3 cut(s) 43, 218, 1130
BceAI ACGGC 1 cut(s) 1191
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 1 cut(s) 191
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 1 cut(s) 173
BfaI CTAG 2 cut(s) 410, 530
BisI GCNGC 3 cut(s) 133, 1047, 1344
BlpI GCTNAGC 1 cut(s) 1124
BlsI GCNGC 3 cut(s) 134, 1048, 1345
Bme1390I CCNGG 2 cut(s) 173, 191
Bme18I GGWCC 2 cut(s) 32, 206
BmeT110I CYCGRG 2 cut(s) 557, 953
BmgT120I GGNCC 2 cut(s) 32, 206
BmiI GGNNCC 3 cut(s) 208, 602, 1072
BmrFI CCNGG 2 cut(s) 173, 191
BmsI GCATC 3 cut(s) 61, 144, 609
Bpu1102I GCTNAGC 1 cut(s) 1124
BpuMI CCSGG 1 cut(s) 173
BsaJI CCNNGG 6 cut(s) 189, 240, 545, 1015, 1066, 1283
Bse118I RCCGGY 1 cut(s) 869
Bse1I ACTGG 2 cut(s) 1124, 1336
Bse3DI GCAATG 2 cut(s) 520, 647
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 6 cut(s) 189, 240, 545, 1015, 1066, 1283
BseGI GGATG 2 cut(s) 210, 1141
BseMI GCAATG 2 cut(s) 520, 647
BseMII CTCAG 2 cut(s) 318, 1115
BseNI ACTGG 2 cut(s) 1124, 1336
BseXI GCAGC 2 cut(s) 119, 1330
BsgI GTGCAG 1 cut(s) 48
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 869
BshNI GGYRCC 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 1200
BsiHKCI CYCGRG 2 cut(s) 557, 953
BsiSI CCGG 3 cut(s) 160, 173, 870
BsnI GGCC 1 cut(s) 869
BsoBI CYCGRG 2 cut(s) 557, 953
Bsp1286I GDGCHC 1 cut(s) 1200
Bsp143I GATC 5 cut(s) 56, 370, 727, 928, 957
Bsp1720I GCTNAGC 1 cut(s) 1124
Bsp19I CCATGG 3 cut(s) 240, 1015, 1283
BspACI CCGC 3 cut(s) 256, 604, 1047
BspANI GGCC 1 cut(s) 869
BspCNI CTCAG 2 cut(s) 319, 1116
BspFNI CGCG 1 cut(s) 342
BspLI GGNNCC 3 cut(s) 208, 602, 1072
BspT107I GGYRCC 1 cut(s) 600
BsrDI GCAATG 2 cut(s) 520, 647
BsrFI RCCGGY 1 cut(s) 869
BsrI ACTGG 2 cut(s) 1124, 1336
BssAI RCCGGY 1 cut(s) 869
BssECI CCNNGG 6 cut(s) 189, 240, 545, 1015, 1066, 1283
BssMI GATC 5 cut(s) 56, 370, 727, 928, 957
BssT1I CCWWGG 5 cut(s) 240, 545, 1015, 1066, 1283
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 4 cut(s) 250, 416, 628, 1174
BstC8I GCNNGC 3 cut(s) 871, 1251, 1341
BstDEI CTNAG 2 cut(s) 327, 1124
BstDSI CCRYGG 3 cut(s) 240, 1015, 1283
BstF5I GGATG 2 cut(s) 210, 1141
BstFNI CGCG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 5 cut(s) 59, 373, 730, 931, 960
BstMBI GATC 5 cut(s) 56, 370, 727, 928, 957
BstMWI GCNNNNNNNGC 1 cut(s) 903
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 2 cut(s) 171, 189
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 119, 1330
BsuRI GGCC 1 cut(s) 869
BtgI CCRYGG 3 cut(s) 240, 1015, 1283
BtgZI GCGATG 1 cut(s) 1143
BtsCI GGATG 2 cut(s) 210, 1141
BtsI GCAGTG 2 cut(s) 904, 1013
BtsIMutI CAGTG 4 cut(s) 904, 1013, 1170, 1329
Cac8I GCNNGC 3 cut(s) 871, 1251, 1341
CfoI GCGC 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 869
Cfr13I GGNCC 2 cut(s) 32, 206
Csp6I GTAC 4 cut(s) 589, 614, 1175, 1186
CviAII CATG 5 cut(s) 107, 241, 487, 1016, 1284
CviQI GTAC 4 cut(s) 589, 614, 1175, 1186
DdeI CTNAG 2 cut(s) 327, 1124
DpnI GATC 5 cut(s) 58, 372, 729, 930, 959
DpnII GATC 5 cut(s) 56, 370, 727, 928, 957
DraI TTTAAA 2 cut(s) 235, 267
Eco130I CCWWGG 5 cut(s) 240, 545, 1015, 1066, 1283
Eco47I GGWCC 2 cut(s) 32, 206
Eco57I CTGAAG 1 cut(s) 124
Eco88I CYCGRG 2 cut(s) 557, 953
EcoO109I RGGNCCY 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 920
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 5 cut(s) 240, 545, 1015, 1066, 1283
EcoT22I ATGCAT 2 cut(s) 76, 1089
ErhI CCWWGG 5 cut(s) 240, 545, 1015, 1066, 1283
FaeI CATG 5 cut(s) 110, 244, 490, 1019, 1287
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FatI CATG 5 cut(s) 106, 240, 486, 1015, 1283
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 133, 1047, 1344
FokI GGATG 2 cut(s) 197, 1148
Fsp4HI GCNGC 3 cut(s) 133, 1047, 1344
FspBI CTAG 2 cut(s) 410, 530
GlaI GCGC 1 cut(s) 343
GluI GCNGC 3 cut(s) 133, 1047, 1344
HaeIII GGCC 1 cut(s) 869
HapII CCGG 3 cut(s) 160, 173, 870
HhaI GCGC 1 cut(s) 344
Hin1II CATG 5 cut(s) 110, 244, 490, 1019, 1287
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HindIII AAGCTT 3 cut(s) 98, 711, 1157
HinfI GANTC 2 cut(s) 110, 833
HpaII CCGG 3 cut(s) 160, 173, 870
HphI GGTGA 4 cut(s) 105, 236, 616, 762
Hpy166II GTNNAC 1 cut(s) 969
Hpy188III TCNNGA 6 cut(s) 530, 557, 559, 953, 955, 1097
Hpy8I GTNNAC 1 cut(s) 969
HpyAV CCTTC 3 cut(s) 284, 1309, 1328
HpyCH4III ACNGT 4 cut(s) 250, 416, 628, 1174
HpyCH4IV ACGT 1 cut(s) 612
HpyF10VI GCNNNNNNNGC 1 cut(s) 903
HpyF3I CTNAG 2 cut(s) 327, 1124
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 5 cut(s) 110, 244, 490, 1019, 1287
HspAI GCGC 1 cut(s) 342
KroI GCCGGC 1 cut(s) 869
KroNI GCCGGC 1 cut(s) 871
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 5 cut(s) 56, 370, 727, 928, 957
Lsp1109I GCAGC 2 cut(s) 119, 1330
LweI GCATC 3 cut(s) 61, 144, 609
MaeI CTAG 2 cut(s) 410, 530
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 3 cut(s) 322, 386, 1231
MalI GATC 5 cut(s) 58, 372, 729, 930, 959
MboI GATC 5 cut(s) 56, 370, 727, 928, 957
MboII GAAGA 6 cut(s) 130, 329, 475, 674, 1085, 1141
MhlI GDGCHC 1 cut(s) 1200
MlyI GAGTC 1 cut(s) 842
MmeI TCCRAC 1 cut(s) 526
Mph1103I ATGCAT 2 cut(s) 76, 1089
MroNI GCCGGC 1 cut(s) 869
MroXI GAANNNNTTC 3 cut(s) 435, 446, 766
MseI TTAA 6 cut(s) 234, 266, 810, 876, 938, 1370
MspI CCGG 3 cut(s) 160, 173, 870
MspR9I CCNGG 2 cut(s) 173, 191
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 903
NaeI GCCGGC 1 cut(s) 871
NciI CCSGG 1 cut(s) 173
NcoI CCATGG 3 cut(s) 240, 1015, 1283
NdeI CATATG 1 cut(s) 670
NdeII GATC 5 cut(s) 56, 370, 727, 928, 957
NgoMIV GCCGGC 1 cut(s) 869
NlaIII CATG 5 cut(s) 110, 244, 490, 1019, 1287
NlaIV GGNNCC 3 cut(s) 208, 602, 1072
NmuCI GTSAC 1 cut(s) 386
NsiI ATGCAT 2 cut(s) 76, 1089
PaeR7I CTCGAG 2 cut(s) 557, 953
PdiI GCCGGC 1 cut(s) 871
PdmI GAANNNNTTC 3 cut(s) 435, 446, 766
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 134, 1048, 1345
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
PpuMI RGGWCCY 1 cut(s) 206
PsiI TTATAA 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 3 cut(s) 208, 602, 1072
PspPI GGNCC 2 cut(s) 32, 206
PspPPI RGGWCCY 1 cut(s) 206
RsaI GTAC 4 cut(s) 590, 615, 1176, 1187
RsaNI GTAC 4 cut(s) 589, 614, 1175, 1186
SaqAI TTAA 6 cut(s) 234, 266, 810, 876, 938, 1370
SatI GCNGC 3 cut(s) 133, 1047, 1344
Sau3AI GATC 5 cut(s) 56, 370, 727, 928, 957
Sau96I GGNCC 2 cut(s) 32, 206
SchI GAGTC 1 cut(s) 842
ScrFI CCNGG 2 cut(s) 173, 191
SduI GDGCHC 1 cut(s) 1200
SfaNI GCATC 3 cut(s) 61, 144, 609
Sfr274I CTCGAG 2 cut(s) 557, 953
SinI GGWCC 2 cut(s) 32, 206
SlaI CTCGAG 2 cut(s) 557, 953
SmlI CTYRAG 2 cut(s) 557, 953
SmoI CTYRAG 2 cut(s) 557, 953
SsiI CCGC 3 cut(s) 256, 604, 1047
SspI AATATT 2 cut(s) 10, 295
SspMI CTAG 2 cut(s) 410, 530
StyD4I CCNGG 2 cut(s) 171, 189
StyI CCWWGG 5 cut(s) 240, 545, 1015, 1066, 1283
TaaI ACNGT 4 cut(s) 250, 416, 628, 1174
TaiI ACGT 1 cut(s) 615
TaqI TCGA 4 cut(s) 59, 290, 558, 954
TaqII GACCGA 1 cut(s) 986
TatI WGTACW 1 cut(s) 1174
TauI GCSGC 1 cut(s) 1049
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 6 cut(s) 234, 266, 810, 876, 938, 1370
Tru9I TTAA 6 cut(s) 234, 266, 810, 876, 938, 1370
TscAI CASTG 4 cut(s) 904, 1013, 1177, 1336
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 2 cut(s) 132, 1343
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 4 cut(s) 412, 723, 1044, 1070
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 4 cut(s) 904, 1013, 1177, 1336
VpaK11BI GGWCC 2 cut(s) 32, 206
XapI RAATTY 6 cut(s) 230, 497, 739, 793, 878, 920
XbaI TCTAGA 1 cut(s) 529
XhoI CTCGAG 2 cut(s) 557, 953
XmnI GAANNNNTTC 3 cut(s) 435, 446, 766
XspI CTAG 2 cut(s) 410, 530
Zsp2I ATGCAT 2 cut(s) 76, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.