Rorug02G0500300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000188
Physical Location & Seq
Reverse (-)
63278179 .. 63297965
19787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0500300.1

Sequence Viewer

Length: 882 bp
ATGTTCAAGCTGACGATACATGAGATTGTTGGCATTGTGCAAGAGGTTGGTCCAAATGTTCATCGCTTCAAAGTTGGCGACCATGTCGGAGTTGGAACCTATACCGACTCATGTAGAGACTGCGAGTATTGTAACGATGGCCAAGAAAATTACTGTGATAAAGAAGCAGTTTACACTTATAACCGTTTTGATTATGATGGTACAATTACCCATGGAGGATTCTCCACTTTCACTGTTGTCCATGAAAGGTACTACTTTAAAATACCAGAAGAGTATCCATTGGCTTCAGCAGCACCACTGCTTTGTGCTAGCATTACTGTTTACTCTCCCATGATGGACCATAAGATGAACCAACCTGGGAAATCCCTAGGCGTGATCGACCTTGGCGGTCTTGGTCACATGGCTGTGAAATTCGGCAAGGCTTTGGGACTCAAGGTAACTGTTTTCAGCACAAGTATGTCAAAGAAAGACGAAGCTCTAACTCTGCTTGGAGCAGACAATTTCGTCATATCATCCAACCAAGACCAAATGAAGGCACTAGCTAAGTCACTTGACTTTATCATTGACACAGCATCTGGTGATCACCCATTCGACGAGTACATGTCACTACTGAAGACTGCTGGTGTTCTAGTCCTCGTGGGTGCCCCAAGTGAAATCAAATTGAGCCCTCTAAGCCTTATTATGGGCAAGAGATCAATTACTGGTAGCGCATCAGGTGGTACAAAACCAATTCAAGAAATGATAGACTTCTGTGCTGCTCACAAAATATATCCAAACATAGAAGTTGTTCCAATTGAGTATGTGAATGAAGCTGTTGAGCGAATCATAAAAAAGGATGTTAAGTACCGATTTGTGATAGATATTGAAAATTCCTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

293

Amino Acids

32.3

Weight (kDa)

5.72

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 7 - 90 7.3e-16 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 130 - 252 2.9e-20 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 163 - 275 5.4e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000188)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02500 FvH4_4g03110 FvH4_5g11900 FvH4_5g11910 FvH4_5g19510 FvH4_6g53141 FvH4_6g53141 FvH4_6g53143 FvH4_6g53143 FvH4_6g53260 FvH4_6g53260 FvH4_6g53260 FvH4_6g53280
malus_domestica MD00G1110800.v1.1 MD00G1111300.v1.1 MD08G1225300.v1.1 MD08G1225500.v1.1 MD08G1225700.v1.1 MD09G1013500.v1.1 MD09G1013700.v1.1 MD09G1013800.v1.1 MD09G1014000.v1.1
prunus_persica Prupe.1G028300_v2.0.a1 Prupe.1G029300_v2.0.a1 Prupe.1G029400_v2.0.a1 Prupe.1G029800_v2.0.a1 Prupe.1G165900_v2.0.a1 Prupe.3G303500_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029100_v2.0.a1 Prupe.5G029200_v2.0.a1 Prupe.7G037400_v2.0.a1
pyrus_communis pycom08g19660 pycom08g19690 pycom08g19710 pycom08g19720 pycom111g01160 pycom111g01170 pycom17g01140
rosa_chinensis RchiOBHm_Chr2g0111831 RchiOBHm_Chr2g0138211 RchiOBHm_Chr2g0163091 RchiOBHm_Chr2g0175051 RchiOBHm_Chr2g0175141 RchiOBHm_Chr2g0175171 RchiOBHm_Chr2g0175191 RchiOBHm_Chr3g0483151 RchiOBHm_Chr3g0483161 RchiOBHm_Chr3g0483171 RchiOBHm_Chr4g0389941 RchiOBHm_Chr4g0411021 RchiOBHm_Chr4g0411031 RchiOBHm_Chr4g0411041 RchiOBHm_Chr5g0041861 RchiOBHm_Chr5g0058261 RchiOBHm_Chr5g0065661 RchiOBHm_Chr5g0070431 RchiOBHm_Chr7g0185221 RchiOBHm_Chr7g0185581 RchiOBHm_Chr7g0185591 RchiOBHm_Chr7g0185611 RchiOBHm_Chr7g0185641
rosa_laevigata RLG00000004895 RLG00000004896 RLG00000004897 RLG00000008379 RLG00000021370 RLG00000022309 RLG00000034099 RLG00000035811 RLG00000036132 RLG00000036450
rosa_multiflora Rmu_co8229993.1_g000001 Rmu_co8360165.1_g000001 Rmu_co8461695.1_g000001 Rmu_sc0000213.1_g000004 Rmu_sc0000235.1_g000066 Rmu_sc0000367.1_g000060 Rmu_sc0000861.1_g000057 Rmu_sc0000861.1_g000068 Rmu_sc0001244.1_g000011 Rmu_sc0001244.1_g000013 Rmu_sc0001323.1_g000018 Rmu_sc0002180.1_g000032 Rmu_sc0002280.1_g000009 Rmu_sc0003226.1_g000095 Rmu_sc0003226.1_g000096 Rmu_sc0003555.1_g000010 Rmu_sc0003555.1_g000018 Rmu_sc0003920.1_g000011 Rmu_sc0003984.1_g000012 Rmu_sc0004336.1_g000026 Rmu_sc0006027.1_g000005 Rmu_sc0006570.1_g000004 Rmu_sc0007961.1_g000010 Rmu_sc0008747.1_g000001 Rmu_sc0008834.1_g000005 Rmu_sc0010190.1_g000004 Rmu_sc0010190.1_g000007 Rmu_sc0010190.1_g000008 Rmu_sc0010642.1_g000012 Rmu_sc0011497.1_g000002
rosa_roxburghii Rroxscaffold_1G00010770 Rroxscaffold_1G00010780 Rroxscaffold_1G00011860 Rroxscaffold_1G00015470 Rroxscaffold_1G00038670 Rroxscaffold_2G00077520 Rroxscaffold_2G00077550 Rroxscaffold_2G00106420 Rroxscaffold_3G00268750 Rroxscaffold_3G00268770 Rroxscaffold_3G00268780 Rroxscaffold_3G00268790 Rroxscaffold_3G00269130 Rroxscaffold_5G00336020 Rroxscaffold_5G00343150 Rroxscaffold_5G00355700
rosa_rugosa Rorug02G0500300 Rorug02G0581200 Rorug03G0204700 Rorug03G0327300 Rorug05G0196100 Rorug05G0196400 Rorug05G0405900 Rorug05G0406000 Rorug06G0467000 Rorug06G0467000 Rorug06G0470200 Rorug06G0470200 Rorug07G0180500
rosa_samantha Rh2AG566700 Rh2AG661900 Rh2AG663200 Rh3CG286400 Rh3CG286500 Rh3CG366100 Rh4DG024200 Rh4DG076600 Rh4DG160000 Rh4DG160100 Rh5BG286800 Rh5DG295500 Rh5DG461000 Rh7DG072100 Rh7DG075500
rosa_wichuraiana Rw1G002310 Rw1G015820 Rw2G046920 Rw2G054280 Rw3G022880 Rw3G029170 Rw4G002330 Rw4G013670 Rw5G026420 Rw5G026440 Rw5G040490 Rw5G042130 Rw7G006300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AasI GACNNNNNNGTC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 641
AciI CCGC 1 cut(s) 387
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 2 cut(s) 410, 868
AcuI CTGAAG 2 cut(s) 270, 632
AfaI GTAC 5 cut(s) 202, 251, 599, 721, 845
AfiI CCNNNNNNNGG 2 cut(s) 532, 682
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 5 cut(s) 7, 70, 734, 866, 877
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 4 cut(s) 10, 476, 542, 812
AluI AGCT 4 cut(s) 10, 476, 542, 812
Alw26I GTCTC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 575
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 290, 755
ApoI RAATTY 2 cut(s) 410, 868
Asp700I GAANNNNTTC 1 cut(s) 786
AspA2I CCTAGG 1 cut(s) 367
AspLEI GCGC 1 cut(s) 710
AspS9I GGNCC 2 cut(s) 50, 337
AsuHPI GGTGA 2 cut(s) 575, 590
AsuNHI GCTAGC 1 cut(s) 308
AvaII GGWCC 2 cut(s) 50, 337
AvrII CCTAGG 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 646
BalI TGGCCA 1 cut(s) 141
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 668
BauI CACGAG 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 2 cut(s) 302, 742
BccI CCATC 3 cut(s) 131, 191, 328
BciT130I CCWGG 1 cut(s) 357
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 4 cut(s) 309, 368, 539, 629
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 2 cut(s) 291, 756
BlnI CCTAGG 1 cut(s) 367
BlsI GCNGC 2 cut(s) 292, 757
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 2 cut(s) 50, 337
BmgT120I GGNCC 2 cut(s) 50, 337
BmiI GGNNCC 2 cut(s) 97, 643
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 2 cut(s) 581, 719
BmtI GCTAGC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 620
BpuEI CTTGAG 1 cut(s) 416
BsaJI CCNNGG 4 cut(s) 211, 356, 367, 382
Bsc4I CCNNNNNNNGG 2 cut(s) 532, 682
Bse1I ACTGG 1 cut(s) 706
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 4 cut(s) 211, 356, 367, 382
BseGI GGATG 2 cut(s) 512, 841
BseLI CCNNNNNNNGG 2 cut(s) 532, 682
BseNI ACTGG 1 cut(s) 706
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 2 cut(s) 302, 742
BshFI GGCC 1 cut(s) 141
BshNI GGYRCC 1 cut(s) 641
BslFI GGGAC 1 cut(s) 441
BslI CCNNNNNNNGG 2 cut(s) 532, 682
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 441
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 646, 668
Bsp143I GATC 3 cut(s) 375, 580, 692
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 1 cut(s) 387
BspANI GGCC 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 97, 643
BspOI GCTAGC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 641
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 4 cut(s) 211, 356, 367, 382
BssMI GATC 3 cut(s) 375, 580, 692
BssSI CACGAG 1 cut(s) 635
BssT1I CCWWGG 3 cut(s) 211, 367, 382
Bst2BI CACGAG 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 5 cut(s) 155, 185, 235, 319, 442
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 2 cut(s) 543, 671
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 2 cut(s) 512, 841
BstHHI GCGC 1 cut(s) 710
BstKTI GATC 3 cut(s) 378, 583, 695
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 3 cut(s) 375, 580, 692
BstMWI GCNNNNNNNGC 2 cut(s) 290, 672
BstNI CCWGG 1 cut(s) 357
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 646
BstV1I GCAGC 2 cut(s) 302, 742
BstV2I GAAGAC 1 cut(s) 620
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 512, 841
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 2 cut(s) 231, 296
Cac8I GCNNGC 1 cut(s) 310
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 1 cut(s) 710
Cfr13I GGNCC 2 cut(s) 50, 337
Csp6I GTAC 5 cut(s) 201, 250, 598, 720, 844
CviAII CATG 8 cut(s) 20, 83, 111, 212, 242, 331, 400, 601
CviQI GTAC 5 cut(s) 201, 250, 598, 720, 844
DdeI CTNAG 2 cut(s) 543, 671
DpnI GATC 3 cut(s) 377, 582, 694
DpnII GATC 3 cut(s) 375, 580, 692
DraI TTTAAA 1 cut(s) 259
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 211, 367, 382
Eco24I GRGCYC 1 cut(s) 668
Eco47I GGWCC 2 cut(s) 50, 337
Eco57I CTGAAG 2 cut(s) 270, 632
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 211, 367, 382
EcoT38I GRGCYC 1 cut(s) 668
ErhI CCWWGG 3 cut(s) 211, 367, 382
FaeI CATG 8 cut(s) 23, 86, 114, 215, 245, 334, 403, 604
FaqI GGGAC 1 cut(s) 441
FatI CATG 8 cut(s) 19, 82, 110, 211, 241, 330, 399, 600
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 2 cut(s) 291, 756
FokI GGATG 2 cut(s) 499, 848
FriOI GRGCYC 1 cut(s) 668
Fsp4HI GCNGC 2 cut(s) 291, 756
FspBI CTAG 4 cut(s) 309, 368, 539, 629
GlaI GCGC 1 cut(s) 709
GluI GCNGC 2 cut(s) 291, 756
HaeIII GGCC 1 cut(s) 141
HhaI GCGC 1 cut(s) 710
Hin1II CATG 8 cut(s) 23, 86, 114, 215, 245, 334, 403, 604
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HinfI GANTC 4 cut(s) 107, 219, 429, 822
HphI GGTGA 2 cut(s) 575, 590
Hpy166II GTNNAC 2 cut(s) 172, 322
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 1 cut(s) 734
Hpy8I GTNNAC 2 cut(s) 172, 322
Hpy99I CGWCG 1 cut(s) 596
HpyAV CCTTC 1 cut(s) 526
HpyCH4III ACNGT 5 cut(s) 155, 185, 235, 319, 442
HpyCH4V TGCA 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 672
HpyF3I CTNAG 2 cut(s) 543, 671
Hsp92II CATG 8 cut(s) 23, 86, 114, 215, 245, 334, 403, 604
HspAI GCGC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 3 cut(s) 375, 580, 692
LmnI GCTCC 1 cut(s) 491
LpnPI CCDG 7 cut(s) 279, 342, 369, 561, 606, 687, 699
Lsp1109I GCAGC 2 cut(s) 302, 742
LweI GCATC 2 cut(s) 581, 719
MaeI CTAG 4 cut(s) 309, 368, 539, 629
MaeIII GTNAC 5 cut(s) 131, 395, 436, 546, 603
MalI GATC 3 cut(s) 377, 582, 694
MboI GATC 3 cut(s) 375, 580, 692
MboII GAAGA 2 cut(s) 281, 625
MfeI CAATTG 1 cut(s) 792
MhlI GDGCHC 2 cut(s) 646, 668
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 9 cut(s) 148, 204, 410, 499, 659, 696, 729, 792, 868
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 2 cut(s) 101, 423
MmeI TCCRAC 3 cut(s) 67, 73, 540
MnlI CCTC 4 cut(s) 37, 209, 644, 678
Mox20I TGGCCA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 141
MseI TTAA 2 cut(s) 258, 840
MslI CAYNNNNRTG 2 cut(s) 404, 455
Msp20I TGGCCA 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 357
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 290, 672
NcoI CCATGG 1 cut(s) 211
NdeII GATC 3 cut(s) 375, 580, 692
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 8 cut(s) 23, 86, 114, 215, 245, 334, 403, 604
NlaIV GGNNCC 2 cut(s) 97, 643
NmuCI GTSAC 3 cut(s) 395, 546, 603
NspI RCATGY 1 cut(s) 604
PciI ACATGT 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 2 cut(s) 219, 822
PflFI GACNNNGTC 1 cut(s) 83
PkrI GCNGC 2 cut(s) 292, 757
PleI GAGTC 2 cut(s) 101, 423
PpsI GAGTC 2 cut(s) 101, 423
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 2 cut(s) 97, 643
PspPI GGNCC 2 cut(s) 50, 337
PstNI CAGNNNCTG 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 83
RsaI GTAC 5 cut(s) 202, 251, 599, 721, 845
RsaNI GTAC 5 cut(s) 201, 250, 598, 720, 844
RseI CAYNNNNRTG 2 cut(s) 404, 455
SaqAI TTAA 2 cut(s) 258, 840
SatI GCNGC 2 cut(s) 291, 756
Sau3AI GATC 3 cut(s) 375, 580, 692
Sau96I GGNCC 2 cut(s) 50, 337
SchI GAGTC 2 cut(s) 101, 423
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 646, 668
SfaNI GCATC 2 cut(s) 581, 719
SinI GGWCC 2 cut(s) 50, 337
SmiMI CAYNNNNRTG 2 cut(s) 404, 455
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 9 cut(s) 148, 204, 410, 499, 659, 696, 729, 792, 868
SsiI CCGC 1 cut(s) 387
SspMI CTAG 4 cut(s) 309, 368, 539, 629
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 3 cut(s) 211, 367, 382
TaaI ACNGT 5 cut(s) 155, 185, 235, 319, 442
TaqI TCGA 2 cut(s) 378, 591
TasI AATT 9 cut(s) 148, 204, 410, 499, 659, 696, 729, 792, 868
TatI WGTACW 1 cut(s) 597
TfiI GAWTC 2 cut(s) 219, 822
Tru1I TTAA 2 cut(s) 258, 840
Tru9I TTAA 2 cut(s) 258, 840
TscAI CASTG 2 cut(s) 238, 303
TseFI GTSAC 3 cut(s) 395, 546, 603
TseI GCWGC 2 cut(s) 290, 755
Tsp45I GTSAC 3 cut(s) 395, 546, 603
TspDTI ATGAA 5 cut(s) 50, 258, 362, 545, 822
TspRI CASTG 2 cut(s) 238, 303
Tth111I GACNNNGTC 1 cut(s) 83
VpaK11BI GGWCC 2 cut(s) 50, 337
XapI RAATTY 2 cut(s) 410, 868
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmaJI CCTAGG 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 4 cut(s) 309, 368, 539, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.