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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 190 |
| AgsI | TTSAA | 1 cut(s) | 112 |
| AluBI | AGCT | 1 cut(s) | 18 |
| AluI | AGCT | 1 cut(s) | 18 |
| Alw21I | GWGCWC | 1 cut(s) | 172 |
| AoxI | GGCC | 1 cut(s) | 8 |
| ApeKI | GCWGC | 2 cut(s) | 80, 144 |
| ApoI | RAATTY | 1 cut(s) | 190 |
| AspLEI | GCGC | 1 cut(s) | 144 |
| AspS9I | GGNCC | 1 cut(s) | 9 |
| AsuHPI | GGTGA | 1 cut(s) | 170 |
| Bbv12I | GWGCWC | 1 cut(s) | 172 |
| BbvI | GCAGC | 2 cut(s) | 67, 156 |
| BccI | CCATC | 1 cut(s) | 155 |
| BclI | TGATCA | 1 cut(s) | 124 |
| BglII | AGATCT | 1 cut(s) | 71 |
| BisI | GCNGC | 2 cut(s) | 81, 145 |
| BlsI | GCNGC | 2 cut(s) | 82, 146 |
| BmgT120I | GGNCC | 1 cut(s) | 9 |
| BmrI | ACTGGG | 1 cut(s) | 212 |
| BmuI | ACTGGG | 1 cut(s) | 212 |
| BpuEI | CTTGAG | 1 cut(s) | 232 |
| Bse1I | ACTGG | 1 cut(s) | 207 |
| BseNI | ACTGG | 1 cut(s) | 207 |
| BseXI | GCAGC | 2 cut(s) | 67, 156 |
| BshFI | GGCC | 1 cut(s) | 10 |
| BsiHKAI | GWGCWC | 1 cut(s) | 172 |
| BsnI | GGCC | 1 cut(s) | 10 |
| Bsp1286I | GDGCHC | 1 cut(s) | 172 |
| Bsp143I | GATC | 3 cut(s) | 60, 71, 124 |
| BspANI | GGCC | 1 cut(s) | 10 |
| BsrI | ACTGG | 1 cut(s) | 207 |
| BssMI | GATC | 3 cut(s) | 60, 71, 124 |
| Bst4CI | ACNGT | 1 cut(s) | 238 |
| BstDEI | CTNAG | 1 cut(s) | 174 |
| BstHHI | GCGC | 1 cut(s) | 144 |
| BstKTI | GATC | 3 cut(s) | 63, 74, 127 |
| BstMBI | GATC | 3 cut(s) | 60, 71, 124 |
| BstV1I | GCAGC | 2 cut(s) | 67, 156 |
| BstX2I | RGATCY | 1 cut(s) | 71 |
| BstYI | RGATCY | 1 cut(s) | 71 |
| BsuRI | GGCC | 1 cut(s) | 10 |
| BtsI | GCAGTG | 1 cut(s) | 172 |
| BtsIMutI | CAGTG | 3 cut(s) | 172, 200, 234 |
| CfoI | GCGC | 1 cut(s) | 144 |
| Cfr13I | GGNCC | 1 cut(s) | 9 |
| CviJI | RGCY | 4 cut(s) | 10, 18, 116, 210 |
| CviKI_1 | RGCY | 4 cut(s) | 10, 18, 116, 210 |
| DdeI | CTNAG | 1 cut(s) | 174 |
| DpnI | GATC | 3 cut(s) | 62, 73, 126 |
| DpnII | GATC | 3 cut(s) | 60, 71, 124 |
| FaiI | YATR | 1 cut(s) | 253 |
| FbaI | TGATCA | 1 cut(s) | 124 |
| Fnu4HI | GCNGC | 2 cut(s) | 81, 145 |
| Fsp4HI | GCNGC | 2 cut(s) | 81, 145 |
| GlaI | GCGC | 1 cut(s) | 143 |
| GluI | GCNGC | 2 cut(s) | 81, 145 |
| HaeIII | GGCC | 1 cut(s) | 10 |
| HhaI | GCGC | 1 cut(s) | 144 |
| Hin6I | GCGC | 1 cut(s) | 142 |
| HinP1I | GCGC | 1 cut(s) | 142 |
| HphI | GGTGA | 1 cut(s) | 170 |
| Hpy188III | TCNNGA | 1 cut(s) | 58 |
| HpyAV | CCTTC | 1 cut(s) | 106 |
| HpyCH4III | ACNGT | 1 cut(s) | 238 |
| HpyF3I | CTNAG | 1 cut(s) | 174 |
| HspAI | GCGC | 1 cut(s) | 142 |
| Ksp22I | TGATCA | 1 cut(s) | 124 |
| Kzo9I | GATC | 3 cut(s) | 60, 71, 124 |
| LmnI | GCTCC | 2 cut(s) | 139, 175 |
| LpnPI | CCDG | 3 cut(s) | 71, 113, 188 |
| Lsp1109I | GCAGC | 2 cut(s) | 67, 156 |
| MaeIII | GTNAC | 1 cut(s) | 94 |
| MalI | GATC | 3 cut(s) | 62, 73, 126 |
| MboI | GATC | 3 cut(s) | 60, 71, 124 |
| MboII | GAAGA | 4 cut(s) | 27, 66, 115, 198 |
| MflI | RGATCY | 1 cut(s) | 71 |
| MhlI | GDGCHC | 1 cut(s) | 172 |
| MluCI | AATT | 2 cut(s) | 190, 244 |
| MnlI | CCTC | 3 cut(s) | 34, 129, 200 |
| NdeII | GATC | 3 cut(s) | 60, 71, 124 |
| NmeAIII | GCCGAG | 1 cut(s) | 207 |
| PkrI | GCNGC | 2 cut(s) | 82, 146 |
| PspPI | GGNCC | 1 cut(s) | 9 |
| PsuI | RGATCY | 1 cut(s) | 71 |
| SatI | GCNGC | 2 cut(s) | 81, 145 |
| Sau3AI | GATC | 3 cut(s) | 60, 71, 124 |
| Sau96I | GGNCC | 1 cut(s) | 9 |
| SduI | GDGCHC | 1 cut(s) | 172 |
| SetI | ASST | 1 cut(s) | 20 |
| SgeI | CNNG | 9 cut(s) | 23, 70, 97, 112, 121, 167, 195, 215, 223 |
| SmlI | CTYRAG | 1 cut(s) | 211 |
| SmoI | CTYRAG | 1 cut(s) | 211 |
| Sse9I | AATT | 2 cut(s) | 190, 244 |
| TaaI | ACNGT | 1 cut(s) | 238 |
| TasI | AATT | 2 cut(s) | 190, 244 |
| TscAI | CASTG | 3 cut(s) | 172, 207, 241 |
| TseI | GCWGC | 2 cut(s) | 80, 144 |
| TspDTI | ATGAA | 1 cut(s) | 27 |
| TspRI | CASTG | 3 cut(s) | 172, 207, 241 |
| XapI | RAATTY | 1 cut(s) | 190 |