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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 100 |
| AciI | CCGC | 1 cut(s) | 100 |
| AgsI | TTSAA | 1 cut(s) | 273 |
| AluBI | AGCT | 6 cut(s) | 52, 68, 89, 208, 244, 280 |
| AluI | AGCT | 6 cut(s) | 52, 68, 89, 208, 244, 280 |
| AoxI | GGCC | 1 cut(s) | 138 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 305 |
| AsuC2I | CCSGG | 1 cut(s) | 28 |
| BcnI | CCSGG | 1 cut(s) | 28 |
| BfmI | CTRYAG | 1 cut(s) | 69 |
| Bme1390I | CCNGG | 1 cut(s) | 28 |
| BmrFI | CCNGG | 1 cut(s) | 28 |
| BpmI | CTGGAG | 1 cut(s) | 302 |
| BpuMI | CCSGG | 1 cut(s) | 28 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 8, 38 |
| BseRI | GAGGAG | 1 cut(s) | 110 |
| BshFI | GGCC | 1 cut(s) | 140 |
| BsiSI | CCGG | 1 cut(s) | 28 |
| BsnI | GGCC | 1 cut(s) | 140 |
| Bsp143I | GATC | 2 cut(s) | 193, 252 |
| BspACI | CCGC | 1 cut(s) | 100 |
| BspANI | GGCC | 1 cut(s) | 140 |
| BsrBI | CCGCTC | 1 cut(s) | 100 |
| BssMI | GATC | 2 cut(s) | 193, 252 |
| Bst4CI | ACNGT | 1 cut(s) | 131 |
| BstC8I | GCNNGC | 2 cut(s) | 66, 278 |
| BstDEI | CTNAG | 1 cut(s) | 19 |
| BstKTI | GATC | 2 cut(s) | 196, 255 |
| BstMBI | GATC | 2 cut(s) | 193, 252 |
| BstSCI | CCNGG | 1 cut(s) | 26 |
| BstSFI | CTRYAG | 1 cut(s) | 69 |
| BsuRI | GGCC | 1 cut(s) | 140 |
| Cac8I | GCNNGC | 2 cut(s) | 66, 278 |
| CseI | GACGC | 1 cut(s) | 101 |
| CviAII | CATG | 1 cut(s) | 125 |
| CviJI | RGCY | 8 cut(s) | 52, 68, 89, 140, 208, 244, 263, 280 |
| CviKI_1 | RGCY | 8 cut(s) | 52, 68, 89, 140, 208, 244, 263, 280 |
| DdeI | CTNAG | 1 cut(s) | 19 |
| DpnI | GATC | 2 cut(s) | 195, 254 |
| DpnII | GATC | 2 cut(s) | 193, 252 |
| Eco147I | AGGCCT | 1 cut(s) | 140 |
| Eco32I | GATATC | 1 cut(s) | 82 |
| EcoRV | GATATC | 1 cut(s) | 82 |
| FaeI | CATG | 1 cut(s) | 128 |
| FaiI | YATR | 4 cut(s) | 126, 158, 266, 340 |
| FatI | CATG | 1 cut(s) | 124 |
| FauI | CCCGC | 1 cut(s) | 93 |
| GsuI | CTGGAG | 1 cut(s) | 302 |
| HaeIII | GGCC | 1 cut(s) | 140 |
| HapII | CCGG | 1 cut(s) | 28 |
| HgaI | GACGC | 1 cut(s) | 101 |
| Hin1II | CATG | 1 cut(s) | 128 |
| HinfI | GANTC | 3 cut(s) | 23, 38, 173 |
| HpaII | CCGG | 1 cut(s) | 28 |
| Hpy188I | TCNGA | 5 cut(s) | 120, 135, 226, 300, 333 |
| Hpy99I | CGWCG | 2 cut(s) | 117, 120 |
| HpyAV | CCTTC | 1 cut(s) | 130 |
| HpyCH4III | ACNGT | 1 cut(s) | 131 |
| HpyCH4V | TGCA | 2 cut(s) | 64, 338 |
| HpyF3I | CTNAG | 1 cut(s) | 19 |
| Hsp92II | CATG | 1 cut(s) | 128 |
| Kzo9I | GATC | 2 cut(s) | 193, 252 |
| LmnI | GCTCC | 5 cut(s) | 57, 97, 213, 249, 283 |
| LpnPI | CCDG | 6 cut(s) | 41, 48, 173, 245, 261, 266 |
| MaeIII | GTNAC | 1 cut(s) | 103 |
| MalI | GATC | 2 cut(s) | 195, 254 |
| MbiI | CCGCTC | 1 cut(s) | 100 |
| MboI | GATC | 2 cut(s) | 193, 252 |
| MboII | GAAGA | 3 cut(s) | 33, 203, 261 |
| MlyI | GAGTC | 1 cut(s) | 17 |
| MmeI | TCCRAC | 2 cut(s) | 98, 204 |
| MnlI | CCTC | 2 cut(s) | 88, 151 |
| MroXI | GAANNNNTTC | 1 cut(s) | 305 |
| MseI | TTAA | 1 cut(s) | 213 |
| MspI | CCGG | 1 cut(s) | 28 |
| MspR9I | CCNGG | 1 cut(s) | 28 |
| NciI | CCSGG | 1 cut(s) | 28 |
| NdeII | GATC | 2 cut(s) | 193, 252 |
| NlaIII | CATG | 1 cut(s) | 128 |
| PceI | AGGCCT | 1 cut(s) | 140 |
| PdmI | GAANNNNTTC | 1 cut(s) | 305 |
| PfeI | GAWTC | 2 cut(s) | 38, 173 |
| PleI | GAGTC | 1 cut(s) | 17 |
| PpsI | GAGTC | 1 cut(s) | 17 |
| SaqAI | TTAA | 1 cut(s) | 213 |
| Sau3AI | GATC | 2 cut(s) | 193, 252 |
| SchI | GAGTC | 1 cut(s) | 17 |
| ScrFI | CCNGG | 1 cut(s) | 28 |
| SetI | ASST | 6 cut(s) | 54, 70, 91, 210, 246, 282 |
| SfcI | CTRYAG | 1 cut(s) | 69 |
| SseBI | AGGCCT | 1 cut(s) | 140 |
| SsiI | CCGC | 1 cut(s) | 100 |
| StuI | AGGCCT | 1 cut(s) | 140 |
| StyD4I | CCNGG | 1 cut(s) | 26 |
| TaaI | ACNGT | 1 cut(s) | 131 |
| TaqI | TCGA | 1 cut(s) | 255 |
| TfiI | GAWTC | 2 cut(s) | 38, 173 |
| Tru1I | TTAA | 1 cut(s) | 213 |
| Tru9I | TTAA | 1 cut(s) | 213 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XmnI | GAANNNNTTC | 1 cut(s) | 305 |