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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 355 |
| AciI | CCGC | 1 cut(s) | 263 |
| AclWI | GGATC | 3 cut(s) | 273, 283, 296 |
| AcsI | RAATTY | 2 cut(s) | 407, 505 |
| AcuI | CTGAAG | 1 cut(s) | 521 |
| AgsI | TTSAA | 3 cut(s) | 320, 397, 482 |
| AluBI | AGCT | 4 cut(s) | 8, 206, 298, 385 |
| AluI | AGCT | 4 cut(s) | 8, 206, 298, 385 |
| Alw26I | GTCTC | 1 cut(s) | 363 |
| AlwI | GGATC | 3 cut(s) | 273, 283, 296 |
| Ama87I | CYCGRG | 1 cut(s) | 323 |
| ApoI | RAATTY | 2 cut(s) | 407, 505 |
| AsuHPI | GGTGA | 1 cut(s) | 358 |
| AvaI | CYCGRG | 1 cut(s) | 323 |
| BamHI | GGATCC | 1 cut(s) | 288 |
| BbsI | GAAGAC | 1 cut(s) | 489 |
| BccI | CCATC | 1 cut(s) | 64 |
| BcoDI | GTCTC | 1 cut(s) | 363 |
| BfaI | CTAG | 3 cut(s) | 17, 117, 386 |
| BglII | AGATCT | 1 cut(s) | 371 |
| BmeT110I | CYCGRG | 1 cut(s) | 323 |
| BmiI | GGNNCC | 1 cut(s) | 290 |
| BmrI | ACTGGG | 1 cut(s) | 294 |
| BmsI | GCATC | 2 cut(s) | 240, 451 |
| BmuI | ACTGGG | 1 cut(s) | 294 |
| BpiI | GAAGAC | 1 cut(s) | 489 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 460, 492 |
| BpmI | CTGGAG | 1 cut(s) | 275 |
| Bpu10I | CCTNAGC | 1 cut(s) | 84 |
| BpuEI | CTTGAG | 1 cut(s) | 314 |
| BsaJI | CCNNGG | 2 cut(s) | 309, 342 |
| Bse1I | ACTGG | 1 cut(s) | 289 |
| Bse3DI | GCAATG | 1 cut(s) | 78 |
| BseDI | CCNNGG | 2 cut(s) | 309, 342 |
| BseMI | GCAATG | 1 cut(s) | 78 |
| BseMII | CTCAG | 2 cut(s) | 98, 503 |
| BseNI | ACTGG | 1 cut(s) | 289 |
| BseRI | GAGGAG | 1 cut(s) | 341 |
| BsiHKCI | CYCGRG | 1 cut(s) | 323 |
| BsmAI | GTCTC | 1 cut(s) | 363 |
| BsoBI | CYCGRG | 1 cut(s) | 323 |
| Bsp143I | GATC | 3 cut(s) | 265, 288, 371 |
| Bsp19I | CCATGG | 1 cut(s) | 309 |
| BspACI | CCGC | 1 cut(s) | 263 |
| BspCNI | CTCAG | 2 cut(s) | 97, 502 |
| BspLI | GGNNCC | 1 cut(s) | 290 |
| BspPI | GGATC | 3 cut(s) | 273, 283, 296 |
| BsrDI | GCAATG | 1 cut(s) | 78 |
| BsrI | ACTGG | 1 cut(s) | 289 |
| BssECI | CCNNGG | 2 cut(s) | 309, 342 |
| BssMI | GATC | 3 cut(s) | 265, 288, 371 |
| BssT1I | CCWWGG | 2 cut(s) | 309, 342 |
| BstDEI | CTNAG | 3 cut(s) | 84, 465, 489 |
| BstDSI | CCRYGG | 1 cut(s) | 309 |
| BstKTI | GATC | 3 cut(s) | 268, 291, 374 |
| BstMAI | GTCTC | 1 cut(s) | 363 |
| BstMBI | GATC | 3 cut(s) | 265, 288, 371 |
| BstV2I | GAAGAC | 1 cut(s) | 489 |
| BstX2I | RGATCY | 2 cut(s) | 288, 371 |
| BstYI | RGATCY | 2 cut(s) | 288, 371 |
| BtgI | CCRYGG | 1 cut(s) | 309 |
| CviAII | CATG | 1 cut(s) | 310 |
| CviJI | RGCY | 8 cut(s) | 8, 143, 168, 206, 278, 298, 385, 392 |
| CviKI_1 | RGCY | 8 cut(s) | 8, 143, 168, 206, 278, 298, 385, 392 |
| DdeI | CTNAG | 3 cut(s) | 84, 465, 489 |
| DpnI | GATC | 3 cut(s) | 267, 290, 373 |
| DpnII | GATC | 3 cut(s) | 265, 288, 371 |
| DraI | TTTAAA | 1 cut(s) | 514 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 355 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 355 |
| EciI | GGCGGA | 1 cut(s) | 278 |
| Eco130I | CCWWGG | 2 cut(s) | 309, 342 |
| Eco32I | GATATC | 1 cut(s) | 30 |
| Eco57I | CTGAAG | 1 cut(s) | 521 |
| Eco88I | CYCGRG | 1 cut(s) | 323 |
| EcoRI | GAATTC | 1 cut(s) | 505 |
| EcoRV | GATATC | 1 cut(s) | 30 |
| EcoT14I | CCWWGG | 2 cut(s) | 309, 342 |
| ErhI | CCWWGG | 2 cut(s) | 309, 342 |
| FaeI | CATG | 1 cut(s) | 313 |
| FaiI | YATR | 8 cut(s) | 102, 174, 176, 233, 311, 335, 419, 459 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 494, 526 |
| FatI | CATG | 1 cut(s) | 309 |
| FspBI | CTAG | 3 cut(s) | 17, 117, 386 |
| GsuI | CTGGAG | 1 cut(s) | 275 |
| Hin1II | CATG | 1 cut(s) | 313 |
| HinfI | GANTC | 1 cut(s) | 356 |
| HphI | GGTGA | 1 cut(s) | 358 |
| Hpy166II | GTNNAC | 1 cut(s) | 94 |
| Hpy188I | TCNGA | 5 cut(s) | 49, 153, 270, 441, 501 |
| Hpy188III | TCNNGA | 2 cut(s) | 64, 479 |
| Hpy8I | GTNNAC | 1 cut(s) | 94 |
| HpyF3I | CTNAG | 3 cut(s) | 84, 465, 489 |
| Hsp92II | CATG | 1 cut(s) | 313 |
| Kzo9I | GATC | 3 cut(s) | 265, 288, 371 |
| LmnI | GCTCC | 1 cut(s) | 183 |
| LpnPI | CCDG | 3 cut(s) | 71, 239, 270 |
| LweI | GCATC | 2 cut(s) | 240, 451 |
| MaeI | CTAG | 3 cut(s) | 17, 117, 386 |
| MaeIII | GTNAC | 1 cut(s) | 346 |
| MalI | GATC | 3 cut(s) | 267, 290, 373 |
| MboI | GATC | 3 cut(s) | 265, 288, 371 |
| MboII | GAAGA | 5 cut(s) | 105, 259, 366, 494, 514 |
| MflI | RGATCY | 2 cut(s) | 288, 371 |
| MluCI | AATT | 5 cut(s) | 50, 134, 189, 407, 505 |
| MlyI | GAGTC | 1 cut(s) | 365 |
| MmeI | TCCRAC | 1 cut(s) | 241 |
| MnlI | CCTC | 4 cut(s) | 86, 250, 319, 462 |
| MseI | TTAA | 3 cut(s) | 192, 222, 513 |
| NcoI | CCATGG | 1 cut(s) | 309 |
| NdeII | GATC | 3 cut(s) | 265, 288, 371 |
| NlaIII | CATG | 1 cut(s) | 313 |
| NlaIV | GGNNCC | 1 cut(s) | 290 |
| NmuCI | GTSAC | 1 cut(s) | 346 |
| PaeR7I | CTCGAG | 1 cut(s) | 323 |
| PleI | GAGTC | 1 cut(s) | 364 |
| PpsI | GAGTC | 1 cut(s) | 364 |
| PspN4I | GGNNCC | 1 cut(s) | 290 |
| PspXI | VCTCGAGB | 1 cut(s) | 323 |
| PsuI | RGATCY | 2 cut(s) | 288, 371 |
| SaqAI | TTAA | 3 cut(s) | 192, 222, 513 |
| Sau3AI | GATC | 3 cut(s) | 265, 288, 371 |
| SchI | GAGTC | 1 cut(s) | 365 |
| SetI | ASST | 8 cut(s) | 10, 18, 78, 90, 208, 300, 348, 387 |
| SfaNI | GCATC | 2 cut(s) | 240, 451 |
| Sfr274I | CTCGAG | 1 cut(s) | 323 |
| SlaI | CTCGAG | 1 cut(s) | 323 |
| SmlI | CTYRAG | 2 cut(s) | 293, 323 |
| SmoI | CTYRAG | 2 cut(s) | 293, 323 |
| Sse9I | AATT | 5 cut(s) | 50, 134, 189, 407, 505 |
| SsiI | CCGC | 1 cut(s) | 263 |
| SspMI | CTAG | 3 cut(s) | 17, 117, 386 |
| StyI | CCWWGG | 2 cut(s) | 309, 342 |
| TaqI | TCGA | 2 cut(s) | 26, 324 |
| TasI | AATT | 5 cut(s) | 50, 134, 189, 407, 505 |
| Tru1I | TTAA | 3 cut(s) | 192, 222, 513 |
| Tru9I | TTAA | 3 cut(s) | 192, 222, 513 |
| TseFI | GTSAC | 1 cut(s) | 346 |
| Tsp45I | GTSAC | 1 cut(s) | 346 |
| TspDTI | ATGAA | 2 cut(s) | 350, 441 |
| XapI | RAATTY | 2 cut(s) | 407, 505 |
| XhoI | CTCGAG | 1 cut(s) | 323 |
| XspI | CTAG | 3 cut(s) | 17, 117, 386 |