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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 235 |
| AciI | CCGC | 1 cut(s) | 347 |
| AcsI | RAATTY | 2 cut(s) | 106, 192 |
| AcyI | GRCGYC | 1 cut(s) | 209 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 235 |
| AgsI | TTSAA | 3 cut(s) | 148, 286, 308 |
| AjnI | CCWGG | 1 cut(s) | 75 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 210, 242 |
| AluBI | AGCT | 4 cut(s) | 8, 38, 290, 304 |
| AluI | AGCT | 4 cut(s) | 8, 38, 290, 304 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 82 |
| ApeKI | GCWGC | 2 cut(s) | 35, 301 |
| ApoI | RAATTY | 2 cut(s) | 106, 192 |
| AsuHPI | GGTGA | 1 cut(s) | 78 |
| BbvI | GCAGC | 2 cut(s) | 47, 313 |
| BccI | CCATC | 1 cut(s) | 56 |
| BceAI | ACGGC | 1 cut(s) | 124 |
| BciT130I | CCWGG | 1 cut(s) | 77 |
| BclI | TGATCA | 1 cut(s) | 166 |
| BfaI | CTAG | 3 cut(s) | 101, 338, 352 |
| BisI | GCNGC | 2 cut(s) | 36, 302 |
| BlsI | GCNGC | 2 cut(s) | 37, 303 |
| Bme1390I | CCNGG | 1 cut(s) | 77 |
| BmrFI | CCNGG | 1 cut(s) | 77 |
| BmsI | GCATC | 2 cut(s) | 52, 324 |
| BsaHI | GRCGYC | 1 cut(s) | 209 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 235 |
| BseBI | CCWGG | 1 cut(s) | 77 |
| BseGI | GGATG | 1 cut(s) | 67 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 235 |
| BseXI | GCAGC | 2 cut(s) | 47, 313 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 235 |
| Bsp143I | GATC | 3 cut(s) | 13, 166, 245 |
| BspACI | CCGC | 1 cut(s) | 347 |
| BssMI | GATC | 3 cut(s) | 13, 166, 245 |
| BssNI | GRCGYC | 1 cut(s) | 209 |
| Bst2UI | CCWGG | 1 cut(s) | 77 |
| BstACI | GRCGYC | 1 cut(s) | 209 |
| BstF5I | GGATG | 1 cut(s) | 67 |
| BstKTI | GATC | 3 cut(s) | 16, 169, 248 |
| BstMBI | GATC | 3 cut(s) | 13, 166, 245 |
| BstNI | CCWGG | 1 cut(s) | 77 |
| BstNSI | RCATGY | 1 cut(s) | 262 |
| BstSCI | CCNGG | 1 cut(s) | 75 |
| BstV1I | GCAGC | 2 cut(s) | 47, 313 |
| BtsCI | GGATG | 1 cut(s) | 67 |
| CaiI | CAGNNNCTG | 1 cut(s) | 82 |
| CseI | GACGC | 1 cut(s) | 217 |
| CviAII | CATG | 2 cut(s) | 230, 259 |
| CviJI | RGCY | 7 cut(s) | 8, 38, 80, 252, 280, 290, 304 |
| CviKI_1 | RGCY | 7 cut(s) | 8, 38, 80, 252, 280, 290, 304 |
| DpnI | GATC | 3 cut(s) | 15, 168, 247 |
| DpnII | GATC | 3 cut(s) | 13, 166, 245 |
| EcoRI | GAATTC | 1 cut(s) | 106 |
| EcoRII | CCWGG | 1 cut(s) | 75 |
| FaeI | CATG | 2 cut(s) | 233, 262 |
| FaiI | YATR | 5 cut(s) | 41, 113, 202, 231, 260 |
| FatI | CATG | 2 cut(s) | 229, 258 |
| FbaI | TGATCA | 1 cut(s) | 166 |
| Fnu4HI | GCNGC | 2 cut(s) | 36, 302 |
| FokI | GGATG | 1 cut(s) | 74 |
| Fsp4HI | GCNGC | 2 cut(s) | 36, 302 |
| FspBI | CTAG | 3 cut(s) | 101, 338, 352 |
| GluI | GCNGC | 2 cut(s) | 36, 302 |
| HgaI | GACGC | 1 cut(s) | 217 |
| Hin1I | GRCGYC | 1 cut(s) | 209 |
| Hin1II | CATG | 2 cut(s) | 233, 262 |
| HincII | GTYRAC | 1 cut(s) | 45 |
| HindII | GTYRAC | 1 cut(s) | 45 |
| HinfI | GANTC | 1 cut(s) | 97 |
| HphI | GGTGA | 1 cut(s) | 78 |
| Hpy166II | GTNNAC | 2 cut(s) | 45, 178 |
| Hpy188I | TCNGA | 3 cut(s) | 133, 171, 245 |
| Hpy188III | TCNNGA | 4 cut(s) | 11, 25, 101, 148 |
| Hpy8I | GTNNAC | 2 cut(s) | 45, 178 |
| Hpy99I | CGWCG | 1 cut(s) | 211 |
| HpyAV | CCTTC | 1 cut(s) | 154 |
| HpyCH4V | TGCA | 2 cut(s) | 272, 301 |
| Hsp92I | GRCGYC | 1 cut(s) | 209 |
| Hsp92II | CATG | 2 cut(s) | 233, 262 |
| Ksp22I | TGATCA | 1 cut(s) | 166 |
| Kzo9I | GATC | 3 cut(s) | 13, 166, 245 |
| LmnI | GCTCC | 1 cut(s) | 13 |
| LpnPI | CCDG | 4 cut(s) | 24, 62, 89, 276 |
| Lsp1109I | GCAGC | 2 cut(s) | 47, 313 |
| LweI | GCATC | 2 cut(s) | 52, 324 |
| MaeI | CTAG | 3 cut(s) | 101, 338, 352 |
| MaeIII | GTNAC | 2 cut(s) | 84, 316 |
| MalI | GATC | 3 cut(s) | 15, 168, 247 |
| MboI | GATC | 3 cut(s) | 13, 166, 245 |
| MboII | GAAGA | 4 cut(s) | 60, 163, 189, 343 |
| MluCI | AATT | 4 cut(s) | 106, 118, 192, 273 |
| MmeI | TCCRAC | 1 cut(s) | 96 |
| MnlI | CCTC | 3 cut(s) | 51, 170, 334 |
| MseI | TTAA | 1 cut(s) | 276 |
| MspR9I | CCNGG | 1 cut(s) | 77 |
| MvaI | CCWGG | 1 cut(s) | 77 |
| NdeII | GATC | 3 cut(s) | 13, 166, 245 |
| NlaIII | CATG | 2 cut(s) | 233, 262 |
| NmuCI | GTSAC | 1 cut(s) | 84 |
| NspI | RCATGY | 1 cut(s) | 262 |
| PfeI | GAWTC | 1 cut(s) | 97 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 235 |
| PkrI | GCNGC | 2 cut(s) | 37, 303 |
| Psp6I | CCWGG | 1 cut(s) | 75 |
| PspGI | CCWGG | 1 cut(s) | 75 |
| PstNI | CAGNNNCTG | 1 cut(s) | 82 |
| SaqAI | TTAA | 1 cut(s) | 276 |
| SatI | GCNGC | 2 cut(s) | 36, 302 |
| Sau3AI | GATC | 3 cut(s) | 13, 166, 245 |
| ScrFI | CCNGG | 1 cut(s) | 77 |
| SetI | ASST | 6 cut(s) | 10, 40, 162, 292, 306, 345 |
| SfaNI | GCATC | 2 cut(s) | 52, 324 |
| Sse9I | AATT | 4 cut(s) | 106, 118, 192, 273 |
| SsiI | CCGC | 1 cut(s) | 347 |
| SspMI | CTAG | 3 cut(s) | 101, 338, 352 |
| StyD4I | CCNGG | 1 cut(s) | 75 |
| TaqI | TCGA | 3 cut(s) | 24, 95, 206 |
| TasI | AATT | 4 cut(s) | 106, 118, 192, 273 |
| TfiI | GAWTC | 1 cut(s) | 97 |
| Tru1I | TTAA | 1 cut(s) | 276 |
| Tru9I | TTAA | 1 cut(s) | 276 |
| TseFI | GTSAC | 1 cut(s) | 84 |
| TseI | GCWGC | 2 cut(s) | 35, 301 |
| Tsp45I | GTSAC | 1 cut(s) | 84 |
| TspDTI | ATGAA | 3 cut(s) | 45, 189, 231 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 235 |
| XapI | RAATTY | 2 cut(s) | 106, 192 |
| XbaI | TCTAGA | 1 cut(s) | 100 |
| XceI | RCATGY | 1 cut(s) | 262 |
| XspI | CTAG | 3 cut(s) | 101, 338, 352 |