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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 416 |
| Acc65I | GGTACC | 1 cut(s) | 168 |
| AccB1I | GGYRCC | 1 cut(s) | 168 |
| AciI | CCGC | 1 cut(s) | 12 |
| AclWI | GGATC | 2 cut(s) | 78, 204 |
| AcoI | YGGCCR | 1 cut(s) | 13 |
| AcsI | RAATTY | 1 cut(s) | 425 |
| AcyI | GRCGYC | 1 cut(s) | 42 |
| AfaI | GTAC | 5 cut(s) | 25, 57, 153, 170, 247 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 52 |
| AgsI | TTSAA | 2 cut(s) | 62, 239 |
| AluBI | AGCT | 2 cut(s) | 242, 388 |
| AluI | AGCT | 2 cut(s) | 242, 388 |
| Alw26I | GTCTC | 2 cut(s) | 327, 478 |
| AlwI | GGATC | 2 cut(s) | 78, 204 |
| AoxI | GGCC | 3 cut(s) | 13, 513, 532 |
| ApaI | GGGCCC | 1 cut(s) | 536 |
| ApeKI | GCWGC | 1 cut(s) | 99 |
| ApoI | RAATTY | 1 cut(s) | 425 |
| Asp718I | GGTACC | 1 cut(s) | 168 |
| AspS9I | GGNCC | 2 cut(s) | 532, 533 |
| AsuHPI | GGTGA | 1 cut(s) | 154 |
| AxyI | CCTNAGG | 1 cut(s) | 294 |
| BaeGI | GKGCMC | 1 cut(s) | 536 |
| BanI | GGYRCC | 1 cut(s) | 168 |
| BanII | GRGCYC | 2 cut(s) | 37, 536 |
| BbvI | GCAGC | 1 cut(s) | 111 |
| BclI | TGATCA | 1 cut(s) | 181 |
| BcoDI | GTCTC | 2 cut(s) | 327, 478 |
| BisI | GCNGC | 2 cut(s) | 13, 100 |
| BlsI | GCNGC | 2 cut(s) | 14, 101 |
| BmgT120I | GGNCC | 2 cut(s) | 532, 533 |
| BmiI | GGNNCC | 3 cut(s) | 170, 533, 534 |
| BpuEI | CTTGAG | 1 cut(s) | 115 |
| BsaBI | GATNNNNATC | 1 cut(s) | 306 |
| BsaHI | GRCGYC | 1 cut(s) | 42 |
| BsaJI | CCNNGG | 2 cut(s) | 46, 556 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 52 |
| Bse21I | CCTNAGG | 1 cut(s) | 294 |
| Bse8I | GATNNNNATC | 1 cut(s) | 306 |
| BseDI | CCNNGG | 2 cut(s) | 46, 556 |
| BseGI | GGATG | 2 cut(s) | 10, 112 |
| BseJI | GATNNNNATC | 1 cut(s) | 306 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 52 |
| BseMII | CTCAG | 1 cut(s) | 308 |
| BseRI | GAGGAG | 2 cut(s) | 282, 384 |
| BseSI | GKGCMC | 1 cut(s) | 536 |
| BseXI | GCAGC | 1 cut(s) | 111 |
| BsgI | GTGCAG | 1 cut(s) | 536 |
| BshFI | GGCC | 3 cut(s) | 15, 515, 534 |
| BshNI | GGYRCC | 1 cut(s) | 168 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 52 |
| BsmAI | GTCTC | 2 cut(s) | 327, 478 |
| BsmI | GAATGC | 1 cut(s) | 451 |
| BsnI | GGCC | 3 cut(s) | 15, 515, 534 |
| Bsp120I | GGGCCC | 1 cut(s) | 532 |
| Bsp1286I | GDGCHC | 2 cut(s) | 37, 536 |
| Bsp143I | GATC | 3 cut(s) | 83, 181, 196 |
| Bsp19I | CCATGG | 1 cut(s) | 556 |
| BspACI | CCGC | 1 cut(s) | 12 |
| BspANI | GGCC | 3 cut(s) | 15, 515, 534 |
| BspCNI | CTCAG | 1 cut(s) | 307 |
| BspLI | GGNNCC | 3 cut(s) | 170, 533, 534 |
| BspPI | GGATC | 2 cut(s) | 78, 204 |
| BspT107I | GGYRCC | 1 cut(s) | 168 |
| BssECI | CCNNGG | 2 cut(s) | 46, 556 |
| BssMI | GATC | 3 cut(s) | 83, 181, 196 |
| BssNI | GRCGYC | 1 cut(s) | 42 |
| BssT1I | CCWWGG | 1 cut(s) | 556 |
| BstACI | GRCGYC | 1 cut(s) | 42 |
| BstC8I | GCNNGC | 1 cut(s) | 10 |
| BstDEI | CTNAG | 3 cut(s) | 147, 294, 329 |
| BstDSI | CCRYGG | 2 cut(s) | 46, 556 |
| BstF5I | GGATG | 2 cut(s) | 10, 112 |
| BstKTI | GATC | 3 cut(s) | 86, 184, 199 |
| BstMAI | GTCTC | 2 cut(s) | 327, 478 |
| BstMBI | GATC | 3 cut(s) | 83, 181, 196 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 41 |
| BstNSI | RCATGY | 1 cut(s) | 328 |
| BstSLI | GKGCMC | 1 cut(s) | 536 |
| BstV1I | GCAGC | 1 cut(s) | 111 |
| BstX2I | RGATCY | 1 cut(s) | 83 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 563 |
| BstYI | RGATCY | 1 cut(s) | 83 |
| Bsu36I | CCTNAGG | 1 cut(s) | 294 |
| BsuRI | GGCC | 3 cut(s) | 15, 515, 534 |
| BtgI | CCRYGG | 2 cut(s) | 46, 556 |
| BtsCI | GGATG | 2 cut(s) | 10, 112 |
| BtsI | GCAGTG | 1 cut(s) | 548 |
| BtsIMutI | CAGTG | 1 cut(s) | 548 |
| Cac8I | GCNNGC | 1 cut(s) | 10 |
| Cfr13I | GGNCC | 2 cut(s) | 532, 533 |
| CseI | GACGC | 1 cut(s) | 50 |
| Csp6I | GTAC | 5 cut(s) | 24, 56, 152, 169, 246 |
| CviAII | CATG | 4 cut(s) | 224, 325, 507, 557 |
| CviJI | RGCY | 7 cut(s) | 15, 35, 69, 242, 388, 515, 534 |
| CviKI_1 | RGCY | 7 cut(s) | 15, 35, 69, 242, 388, 515, 534 |
| CviQI | GTAC | 5 cut(s) | 24, 56, 152, 169, 246 |
| DdeI | CTNAG | 3 cut(s) | 147, 294, 329 |
| DpnI | GATC | 3 cut(s) | 85, 183, 198 |
| DpnII | GATC | 3 cut(s) | 83, 181, 196 |
| EaeI | YGGCCR | 1 cut(s) | 13 |
| Eco130I | CCWWGG | 1 cut(s) | 556 |
| Eco147I | AGGCCT | 1 cut(s) | 515 |
| Eco24I | GRGCYC | 2 cut(s) | 37, 536 |
| Eco81I | CCTNAGG | 1 cut(s) | 294 |
| EcoO109I | RGGNCCY | 2 cut(s) | 532, 533 |
| EcoT14I | CCWWGG | 1 cut(s) | 556 |
| EcoT38I | GRGCYC | 2 cut(s) | 37, 536 |
| ErhI | CCWWGG | 1 cut(s) | 556 |
| FaeI | CATG | 4 cut(s) | 227, 328, 510, 560 |
| FatI | CATG | 4 cut(s) | 223, 324, 506, 556 |
| FbaI | TGATCA | 1 cut(s) | 181 |
| Fnu4HI | GCNGC | 2 cut(s) | 13, 100 |
| FokI | GGATG | 2 cut(s) | 17, 99 |
| FriOI | GRGCYC | 2 cut(s) | 37, 536 |
| Fsp4HI | GCNGC | 2 cut(s) | 13, 100 |
| GluI | GCNGC | 2 cut(s) | 13, 100 |
| HaeIII | GGCC | 3 cut(s) | 15, 515, 534 |
| HgaI | GACGC | 1 cut(s) | 50 |
| Hin1I | GRCGYC | 1 cut(s) | 42 |
| Hin1II | CATG | 4 cut(s) | 227, 328, 510, 560 |
| HincII | GTYRAC | 1 cut(s) | 262 |
| HindII | GTYRAC | 1 cut(s) | 262 |
| HinfI | GANTC | 2 cut(s) | 281, 471 |
| HphI | GGTGA | 1 cut(s) | 154 |
| Hpy166II | GTNNAC | 2 cut(s) | 262, 405 |
| Hpy188I | TCNGA | 3 cut(s) | 73, 186, 193 |
| Hpy188III | TCNNGA | 2 cut(s) | 94, 296 |
| Hpy8I | GTNNAC | 2 cut(s) | 262, 405 |
| HpyAV | CCTTC | 2 cut(s) | 269, 505 |
| HpyCH4V | TGCA | 5 cut(s) | 8, 230, 348, 449, 553 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 41 |
| HpyF3I | CTNAG | 3 cut(s) | 147, 294, 329 |
| Hsp92I | GRCGYC | 1 cut(s) | 42 |
| Hsp92II | CATG | 4 cut(s) | 227, 328, 510, 560 |
| KpnI | GGTACC | 1 cut(s) | 172 |
| Ksp22I | TGATCA | 1 cut(s) | 181 |
| Kzo9I | GATC | 3 cut(s) | 83, 181, 196 |
| LpnPI | CCDG | 4 cut(s) | 150, 226, 281, 326 |
| Lsp1109I | GCAGC | 1 cut(s) | 111 |
| MaeIII | GTNAC | 2 cut(s) | 133, 142 |
| MalI | GATC | 3 cut(s) | 85, 183, 198 |
| MboI | GATC | 3 cut(s) | 83, 181, 196 |
| MboII | GAAGA | 2 cut(s) | 308, 311 |
| MflI | RGATCY | 1 cut(s) | 83 |
| MhlI | GDGCHC | 2 cut(s) | 37, 536 |
| MluCI | AATT | 2 cut(s) | 425, 524 |
| MlyI | GAGTC | 1 cut(s) | 480 |
| MmeI | TCCRAC | 1 cut(s) | 448 |
| MnlI | CCTC | 8 cut(s) | 97, 129, 187, 265, 303, 362, 425, 546 |
| MslI | CAYNNNNRTG | 1 cut(s) | 561 |
| Mva1269I | GAATGC | 1 cut(s) | 451 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 41 |
| NcoI | CCATGG | 1 cut(s) | 556 |
| NdeII | GATC | 3 cut(s) | 83, 181, 196 |
| NlaIII | CATG | 4 cut(s) | 227, 328, 510, 560 |
| NlaIV | GGNNCC | 3 cut(s) | 170, 533, 534 |
| NmuCI | GTSAC | 1 cut(s) | 142 |
| NspI | RCATGY | 1 cut(s) | 328 |
| PceI | AGGCCT | 1 cut(s) | 515 |
| PctI | GAATGC | 1 cut(s) | 451 |
| PfeI | GAWTC | 1 cut(s) | 281 |
| PkrI | GCNGC | 2 cut(s) | 14, 101 |
| PleI | GAGTC | 1 cut(s) | 479 |
| PpsI | GAGTC | 1 cut(s) | 479 |
| PsiI | TTATAA | 1 cut(s) | 416 |
| PspN4I | GGNNCC | 3 cut(s) | 170, 533, 534 |
| PspOMI | GGGCCC | 1 cut(s) | 532 |
| PspPI | GGNCC | 2 cut(s) | 532, 533 |
| PsuI | RGATCY | 1 cut(s) | 83 |
| RsaI | GTAC | 5 cut(s) | 25, 57, 153, 170, 247 |
| RsaNI | GTAC | 5 cut(s) | 24, 56, 152, 169, 246 |
| RseI | CAYNNNNRTG | 1 cut(s) | 561 |
| SatI | GCNGC | 2 cut(s) | 13, 100 |
| Sau3AI | GATC | 3 cut(s) | 83, 181, 196 |
| Sau96I | GGNCC | 2 cut(s) | 532, 533 |
| SchI | GAGTC | 1 cut(s) | 480 |
| SduI | GDGCHC | 2 cut(s) | 37, 536 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 561 |
| SmlI | CTYRAG | 1 cut(s) | 94 |
| SmoI | CTYRAG | 1 cut(s) | 94 |
| Sse9I | AATT | 2 cut(s) | 425, 524 |
| SseBI | AGGCCT | 1 cut(s) | 515 |
| SsiI | CCGC | 1 cut(s) | 12 |
| StuI | AGGCCT | 1 cut(s) | 515 |
| StyI | CCWWGG | 1 cut(s) | 556 |
| TaqI | TCGA | 2 cut(s) | 217, 367 |
| TasI | AATT | 2 cut(s) | 425, 524 |
| TatI | WGTACW | 1 cut(s) | 23 |
| TauI | GCSGC | 1 cut(s) | 15 |
| TfiI | GAWTC | 1 cut(s) | 281 |
| TscAI | CASTG | 1 cut(s) | 555 |
| TseFI | GTSAC | 1 cut(s) | 142 |
| TseI | GCWGC | 1 cut(s) | 99 |
| Tsp45I | GTSAC | 1 cut(s) | 142 |
| TspDTI | ATGAA | 3 cut(s) | 101, 149, 308 |
| TspRI | CASTG | 1 cut(s) | 555 |
| XapI | RAATTY | 1 cut(s) | 425 |
| XceI | RCATGY | 1 cut(s) | 328 |