Rmu_co8385689.1_g000001

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8385689.1
OG0000253
Physical Location & Seq
Reverse (-)
2 .. 1171
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8385689.1_g000001.1.cds

Sequence Viewer

Length: 1170 bp
ctgggctatatttaccatggtcttggcctagctgccactgatccagaagggcctggaaattcgaatgtttgctttcccattcattatgttattggttggctaggagaacacttcccctgcttatatagagctcgagccgatagtgacttcccaactaaccatccgtatttggcaagatatgcaggtattgaggccgttgatttggatatttcatcggctagacgtgttttcagaactgatgtttctgtgatttaccgtcctaattcatttattgaacaaagcggttgctttttgactgacgatggtggcctgtcaagtgatcagttcgagttgttggtttgtatgcgctcttcattgttgccggtgagagtgggacgtgatctttgcttggaaccatattatccgaacagatttgctcgccaatttggctttgatcaaggggttccctcaaacagactcagttttggccttaccagaaggcgtcaatgctgcattgaggatttagccaaggcccagcaggtactcttccaaacagataccggctctcgcttttacattccacgttccacttataatgggacatgtacctggacatattgtaggtggtggatgagagcttgtgcaccatacctaggcaggtctgtgtccaatgtttacttgacattgaccaaacgtcgacttgagaaaagagatgatatatttgtgatcaaatccatcagagagttctcttccaatttgcgaaaagaggtcttgttttcagaagaggacataagtaggtcggctgcttatgacaagaatccccaacctgctaaaaggacgaacaggtcgggtgaatatcggaaagcatcttcgagtaattttgaaactaggcattcaaaacgacaacgcaacgagcgacatggcatcaaagaaacttccagagctccatttcaaaatagtggggcagcaaaaactctcaagtgtgctcaatttgagagatacgatggttatgaagatggcagaattgatgatacaacttttcctcaggatgattttgttcaggacagagatgttgccccctgtgatggtgacaaaatcactactccaaggttgaaatcagtaatatcgcgagttaccgaaacaaatggttcagaagtggagacaccaccaaaggacttggaacaaaagtca

Protein Analysis

390

Amino Acids

44.6

Weight (kDa)

8.64

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28912 FvH4_3g31370 FvH4_3g31371 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_6g28991 FvH4_7g16740 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610
malus_domestica MD10G1126100.v1.1 MD10G1312700.v1.1
rosa_chinensis RchiOBHm_Chr6g0276621
rosa_laevigata RLG00000023327 RLG00000023328
rosa_multiflora Rmu_co8195922.1_g000001 Rmu_co8303105.1_g000001 Rmu_co8385689.1_g000001 Rmu_sc0000385.1_g000010 Rmu_sc0000594.1_g000002 Rmu_sc0000857.1_g000010 Rmu_sc0001016.1_g000053 Rmu_sc0001468.1_g000028 Rmu_sc0001548.1_g000047 Rmu_sc0001548.1_g000048 Rmu_sc0002281.1_g000026 Rmu_sc0002281.1_g000027 Rmu_sc0002281.1_g000028 Rmu_sc0002478.1_g000001 Rmu_sc0002513.1_g000014 Rmu_sc0002662.1_g000017 Rmu_sc0003936.1_g000040 Rmu_sc0004084.1_g000047 Rmu_sc0005529.1_g000005 Rmu_sc0005529.1_g000006 Rmu_sc0005728.1_g000010 Rmu_sc0005747.1_g000017 Rmu_sc0005747.1_g000018 Rmu_sc0006385.1_g000001 Rmu_sc0006550.1_g000019 Rmu_sc0006803.1_g000033 Rmu_sc0007102.1_g000011 Rmu_sc0011018.1_g000001 Rmu_sc0011452.1_g000014 Rmu_sc0011805.1_g000001 Rmu_sc0011805.1_g000002 Rmu_sc0012566.1_g000001 Rmu_sc0013807.1_g000007 Rmu_sc0015817.1_g000001 Rmu_sc0015930.1_g000011 Rmu_sc0018873.1_g000003 Rmu_sc0020929.1_g000001
rosa_roxburghii Rroxscaffold_2G00081310 Rroxscaffold_2G00111790 Rroxscaffold_5G00349530 Rroxscaffold_5G00356270 Rroxscaffold_5G00356280 Rroxscaffold_5G00356290
rosa_rugosa Rorug01G0056400 Rorug01G0056500 Rorug02G0247000 Rorug03G0271700 Rorug04G0157900 Rorug05G0221700 Rorug05G0251400 Rorug05G0251400 Rorug06G0087300 Rorug07G0221900 Rorug07G0221900
rosa_samantha Rh1AG073200 Rh1AG073300 Rh1AG124600 Rh1AG124700 Rh1BG091900 Rh1CG031600 Rh1CG072100 Rh1CG072200 Rh1CG118800 Rh1CG276600 Rh1CG276700 Rh1DG077300 Rh2BG192000 Rh2BG330900 Rh2BG331000 Rh2BG517700 Rh2BG517800 Rh2CG188300 Rh2DG120800 Rh2DG323900 Rh2DG420700 Rh2DG433000 Rh2DG529400 Rh2DG529500 Rh3DG358100 Rh3DG358200 Rh4BG129700 Rh4BG129800 Rh4BG129900 Rh4BG166400 Rh4CG032000 Rh4DG024800 Rh4DG039500 Rh5AG223400 Rh5BG244500 Rh5BG244600 Rh5BG244700 Rh5CG363000 Rh5CG366900 Rh6AG117500 Rh6AG188600 Rh6AG188700 Rh6AG188800 Rh6BG111400 Rh6BG140800 Rh6BG178800 Rh6DG124100 Rh6DG210000 Rh7AG441200 Rh7BG296800 Rh7BG413900 Rh7BG414000 Rh7BG452400 Rh7DG234000 Rh7DG234100
rosa_wichuraiana Rw2G019410 Rw5G042140 Rw7G019590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AasI GACNNNNNNGTC 1 cut(s) 823
Acc36I ACCTGC 4 cut(s) 173, 508, 627, 814
AccI GTMKAC 1 cut(s) 676
AccII CGCG 1 cut(s) 1108
AciI CCGC 1 cut(s) 282
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 481
AfaI GTAC 2 cut(s) 522, 586
AfiI CCNNNNNNNGG 2 cut(s) 632, 1064
AflIII ACRYGT 2 cut(s) 223, 581
AgsI TTSAA 5 cut(s) 275, 863, 876, 932, 1093
AhdI GACNNNNNGTC 1 cut(s) 672
AjiI CACGTC 2 cut(s) 224, 377
AjnI CCWGG 2 cut(s) 52, 587
AluBI AGCT 4 cut(s) 32, 131, 617, 923
AluI AGCT 4 cut(s) 32, 131, 617, 923
Alw21I GWGCWC 4 cut(s) 133, 625, 925, 967
Alw26I GTCTC 1 cut(s) 1133
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 6 cut(s) 25, 50, 192, 307, 466, 510
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 4 cut(s) 32, 489, 782, 944
ApoI RAATTY 1 cut(s) 58
ArsI GACNNNNNNTTYG 2 cut(s) 366, 398
AspA2I CCTAGG 1 cut(s) 631
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 50, 511
AsuHPI GGTGA 3 cut(s) 376, 842, 1079
AsuII TTCGAA 1 cut(s) 62
AvaI CYCGRG 1 cut(s) 132
AvrII CCTAGG 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 1023
BaeGI GKGCMC 1 cut(s) 625
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BanII GRGCYC 2 cut(s) 133, 925
BarI GAAGNNNNNNTAC 2 cut(s) 334, 366
Bbv12I GWGCWC 4 cut(s) 133, 625, 925, 967
BbvI GCAGC 4 cut(s) 19, 476, 769, 956
BccI CCATC 6 cut(s) 168, 296, 722, 977, 989, 1058
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 2 cut(s) 54, 589
BclI TGATCA 3 cut(s) 319, 433, 705
BcoDI GTCTC 1 cut(s) 1133
BfaI CTAG 5 cut(s) 29, 101, 219, 632, 867
BfuAI ACCTGC 4 cut(s) 173, 508, 627, 814
BglI GCCNNNNNGGC 1 cut(s) 426
BisI GCNGC 4 cut(s) 33, 490, 783, 945
BlnI CCTAGG 1 cut(s) 631
BlsI GCNGC 4 cut(s) 34, 491, 784, 946
Bme1390I CCNGG 2 cut(s) 54, 589
BmeRI GACNNNNNGTC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 132
BmgBI CACGTC 2 cut(s) 224, 377
BmgT120I GGNCC 2 cut(s) 50, 511
BmiI GGNNCC 2 cut(s) 393, 444
BmrFI CCNGG 2 cut(s) 54, 589
BmsI GCATC 2 cut(s) 854, 912
Bpu14I TTCGAA 1 cut(s) 62
BpuEI CTTGAG 2 cut(s) 701, 941
BsaHI GRCGYC 1 cut(s) 481
BsaJI CCNNGG 4 cut(s) 16, 507, 631, 1085
Bsc4I CCNNNNNNNGG 2 cut(s) 632, 1064
Bse118I RCCGGY 2 cut(s) 361, 539
Bse21I CCTNAGG 1 cut(s) 1023
BseBI CCWGG 2 cut(s) 54, 589
BseDI CCNNGG 4 cut(s) 16, 507, 631, 1085
BseGI GGATG 3 cut(s) 160, 615, 1033
BseLI CCNNNNNNNGG 2 cut(s) 632, 1064
BseMII CTCAG 2 cut(s) 472, 1037
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 4 cut(s) 19, 476, 769, 956
BseYI CCCAGC 1 cut(s) 513
Bsh1236I CGCG 1 cut(s) 1108
BshFI GGCC 6 cut(s) 27, 52, 194, 309, 468, 512
BsiHKAI GWGCWC 4 cut(s) 133, 625, 925, 967
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 2 cut(s) 362, 540
BslFI GGGAC 2 cut(s) 387, 592
BslI CCNNNNNNNGG 2 cut(s) 632, 1064
BsmAI GTCTC 1 cut(s) 1133
BsmFI GGGAC 2 cut(s) 387, 592
BsmI GAATGC 1 cut(s) 871
BsnI GGCC 6 cut(s) 27, 52, 194, 309, 468, 512
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 4 cut(s) 133, 625, 925, 967
Bsp143I GATC 5 cut(s) 40, 319, 379, 433, 705
Bsp19I CCATGG 1 cut(s) 16
Bsp68I TCGCGA 1 cut(s) 1108
BspACI CCGC 1 cut(s) 282
BspANI GGCC 6 cut(s) 27, 52, 194, 309, 468, 512
BspCNI CTCAG 2 cut(s) 471, 1036
BspFNI CGCG 1 cut(s) 1108
BspLI GGNNCC 2 cut(s) 393, 444
BspMI ACCTGC 4 cut(s) 173, 508, 627, 814
BspPI GGATC 1 cut(s) 35
BspQI GCTCTTC 1 cut(s) 355
BspT104I TTCGAA 1 cut(s) 62
BsrFI RCCGGY 2 cut(s) 361, 539
BssAI RCCGGY 2 cut(s) 361, 539
BssECI CCNNGG 4 cut(s) 16, 507, 631, 1085
BssMI GATC 5 cut(s) 40, 319, 379, 433, 705
BssNI GRCGYC 1 cut(s) 481
BssT1I CCWWGG 4 cut(s) 16, 507, 631, 1085
Bst2UI CCWGG 2 cut(s) 54, 589
Bst4CI ACNGT 1 cut(s) 257
Bst6I CTCTTC 4 cut(s) 355, 530, 733, 756
BstACI GRCGYC 1 cut(s) 481
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 2 cut(s) 458, 1023
BstDSI CCRYGG 1 cut(s) 16
BstF5I GGATG 3 cut(s) 160, 615, 1033
BstFNI CGCG 1 cut(s) 1108
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 5 cut(s) 43, 322, 382, 436, 708
BstMAI GTCTC 1 cut(s) 1133
BstMBI GATC 5 cut(s) 40, 319, 379, 433, 705
BstMWI GCNNNNNNNGC 2 cut(s) 179, 426
BstNI CCWGG 2 cut(s) 54, 589
BstNSI RCATGY 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 52, 587
BstSLI GKGCMC 1 cut(s) 625
BstUI CGCG 1 cut(s) 1108
BstV1I GCAGC 4 cut(s) 19, 476, 769, 956
BstXI CCANNNNNNTGG 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 1023
BsuRI GGCC 6 cut(s) 27, 52, 194, 309, 468, 512
BtgI CCRYGG 1 cut(s) 16
BtrI CACGTC 2 cut(s) 224, 377
BtsCI GGATG 3 cut(s) 160, 615, 1033
BtsIMutI CAGTG 1 cut(s) 36
BtuMI TCGCGA 1 cut(s) 1108
BveI ACCTGC 4 cut(s) 173, 508, 627, 814
Cac8I GCNNGC 1 cut(s) 418
CfoI GCGC 1 cut(s) 348
Cfr10I RCCGGY 2 cut(s) 361, 539
Cfr13I GGNCC 2 cut(s) 50, 511
CseI GACGC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 521, 585
CviAII CATG 3 cut(s) 17, 582, 899
CviQI GTAC 2 cut(s) 521, 585
DdeI CTNAG 2 cut(s) 458, 1023
DpnI GATC 5 cut(s) 42, 321, 381, 435, 707
DpnII GATC 5 cut(s) 40, 319, 379, 433, 705
DrdI GACNNNNNNGTC 1 cut(s) 823
DriI GACNNNNNGTC 1 cut(s) 672
DseDI GACNNNNNNGTC 1 cut(s) 823
Eam1104I CTCTTC 4 cut(s) 355, 530, 733, 756
Eam1105I GACNNNNNGTC 1 cut(s) 672
EarI CTCTTC 4 cut(s) 355, 530, 733, 756
Ecl136II GAGCTC 2 cut(s) 131, 923
Eco130I CCWWGG 4 cut(s) 16, 507, 631, 1085
Eco24I GRGCYC 2 cut(s) 133, 925
Eco53kI GAGCTC 2 cut(s) 131, 923
Eco81I CCTNAGG 1 cut(s) 1023
Eco88I CYCGRG 1 cut(s) 132
EcoICRI GAGCTC 2 cut(s) 131, 923
EcoO109I RGGNCCY 1 cut(s) 50
EcoRII CCWGG 2 cut(s) 52, 587
EcoT14I CCWWGG 4 cut(s) 16, 507, 631, 1085
EcoT38I GRGCYC 2 cut(s) 133, 925
ErhI CCWWGG 4 cut(s) 16, 507, 631, 1085
FaeI CATG 3 cut(s) 20, 585, 902
FaqI GGGAC 2 cut(s) 387, 592
FatI CATG 3 cut(s) 16, 581, 898
FbaI TGATCA 3 cut(s) 319, 433, 705
FblI GTMKAC 1 cut(s) 676
Fnu4HI GCNGC 4 cut(s) 33, 490, 783, 945
FokI GGATG 3 cut(s) 147, 622, 1040
FriOI GRGCYC 2 cut(s) 133, 925
Fsp4HI GCNGC 4 cut(s) 33, 490, 783, 945
FspBI CTAG 5 cut(s) 29, 101, 219, 632, 867
GlaI GCGC 1 cut(s) 347
GluI GCNGC 4 cut(s) 33, 490, 783, 945
GsaI CCCAGC 1 cut(s) 517
HaeIII GGCC 6 cut(s) 27, 52, 194, 309, 468, 512
HapII CCGG 2 cut(s) 362, 540
HgaI GACGC 1 cut(s) 470
HhaI GCGC 1 cut(s) 348
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 3 cut(s) 20, 585, 902
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 677
HindII GTYRAC 1 cut(s) 677
HinfI GANTC 2 cut(s) 456, 796
HpaII CCGG 2 cut(s) 362, 540
HphI GGTGA 3 cut(s) 376, 842, 1079
Hpy166II GTNNAC 3 cut(s) 623, 655, 677
Hpy188I TCNGA 6 cut(s) 233, 405, 719, 760, 840, 1132
Hpy188III TCNNGA 5 cut(s) 44, 918, 1025, 1040, 1107
Hpy8I GTNNAC 3 cut(s) 623, 655, 677
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 2 cut(s) 41, 471
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4IV ACGT 4 cut(s) 223, 376, 562, 673
HpyCH4V TGCA 3 cut(s) 182, 492, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 426
HpyF3I CTNAG 2 cut(s) 458, 1023
HpySE526I ACGT 4 cut(s) 223, 376, 562, 673
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 3 cut(s) 20, 585, 902
HspAI GCGC 1 cut(s) 346
Ksp22I TGATCA 3 cut(s) 319, 433, 705
Kzo9I GATC 5 cut(s) 40, 319, 379, 433, 705
LguI GCTCTTC 1 cut(s) 355
LmnI GCTCC 1 cut(s) 928
Lsp1109I GCAGC 4 cut(s) 19, 476, 769, 956
LweI GCATC 2 cut(s) 854, 912
MaeI CTAG 5 cut(s) 29, 101, 219, 632, 867
MaeII ACGT 4 cut(s) 223, 376, 562, 673
MaeIII GTNAC 3 cut(s) 143, 1067, 1111
MalI GATC 5 cut(s) 42, 321, 381, 435, 707
MboI GATC 5 cut(s) 40, 319, 379, 433, 705
MboII GAAGA 6 cut(s) 342, 517, 720, 773, 840, 1004
MhlI GDGCHC 4 cut(s) 133, 625, 925, 967
MluCI AATT 7 cut(s) 58, 262, 422, 733, 856, 968, 1002
MlyI GAGTC 1 cut(s) 450
MnlI CCTC 6 cut(s) 184, 457, 490, 739, 757, 1032
MspI CCGG 2 cut(s) 362, 540
MspR9I CCNGG 2 cut(s) 54, 589
Mva1269I GAATGC 1 cut(s) 871
MvaI CCWGG 2 cut(s) 54, 589
MvnI CGCG 1 cut(s) 1108
MwoI GCNNNNNNNGC 2 cut(s) 179, 426
NcoI CCATGG 1 cut(s) 16
NdeII GATC 5 cut(s) 40, 319, 379, 433, 705
NlaIII CATG 3 cut(s) 20, 585, 902
NlaIV GGNNCC 2 cut(s) 393, 444
NmuCI GTSAC 2 cut(s) 143, 1067
NruI TCGCGA 1 cut(s) 1108
NspI RCATGY 1 cut(s) 585
NspV TTCGAA 1 cut(s) 62
PaeR7I CTCGAG 1 cut(s) 132
PciI ACATGT 1 cut(s) 581
PciSI GCTCTTC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 892
PctI GAATGC 1 cut(s) 871
PfeI GAWTC 1 cut(s) 796
PkrI GCNGC 4 cut(s) 34, 491, 784, 946
PleI GAGTC 1 cut(s) 450
PpsI GAGTC 1 cut(s) 450
PscI ACATGT 1 cut(s) 581
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 133, 925
Psp6I CCWGG 2 cut(s) 52, 587
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 2 cut(s) 52, 587
PspN4I GGNNCC 2 cut(s) 393, 444
PspPI GGNCC 2 cut(s) 50, 511
PspXI VCTCGAGB 1 cut(s) 132
RruI TCGCGA 1 cut(s) 1108
RsaI GTAC 2 cut(s) 522, 586
RsaNI GTAC 2 cut(s) 521, 585
SacI GAGCTC 2 cut(s) 133, 925
SalI GTCGAC 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 355
SatI GCNGC 4 cut(s) 33, 490, 783, 945
Sau3AI GATC 5 cut(s) 40, 319, 379, 433, 705
Sau96I GGNCC 2 cut(s) 50, 511
SchI GAGTC 1 cut(s) 450
ScrFI CCNGG 2 cut(s) 54, 589
SduI GDGCHC 4 cut(s) 133, 625, 925, 967
SfaNI GCATC 2 cut(s) 854, 912
Sfr274I CTCGAG 1 cut(s) 132
SfuI TTCGAA 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 132
SmlI CTYRAG 3 cut(s) 132, 680, 956
SmoI CTYRAG 3 cut(s) 132, 680, 956
Sse9I AATT 7 cut(s) 58, 262, 422, 733, 856, 968, 1002
SsiI CCGC 1 cut(s) 282
SspMI CTAG 5 cut(s) 29, 101, 219, 632, 867
SstI GAGCTC 2 cut(s) 133, 925
StyD4I CCNGG 2 cut(s) 52, 587
StyI CCWWGG 4 cut(s) 16, 507, 631, 1085
TaaI ACNGT 1 cut(s) 257
TaiI ACGT 4 cut(s) 226, 379, 565, 676
TaqI TCGA 5 cut(s) 62, 133, 327, 676, 851
TasI AATT 7 cut(s) 58, 262, 422, 733, 856, 968, 1002
TfiI GAWTC 1 cut(s) 796
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 2 cut(s) 143, 1067
TseI GCWGC 4 cut(s) 32, 489, 782, 944
Tsp45I GTSAC 2 cut(s) 143, 1067
TspDTI ATGAA 5 cut(s) 71, 201, 255, 342, 1005
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 43
VneI GTGCAC 1 cut(s) 621
XapI RAATTY 1 cut(s) 58
XceI RCATGY 1 cut(s) 585
XhoI CTCGAG 1 cut(s) 132
XmaJI CCTAGG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 676
XspI CTAG 5 cut(s) 29, 101, 219, 632, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.