Rh5BG244500

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000253
Physical Location & Seq
Reverse (-)
29615378 .. 29617045
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG244500.1

Sequence Viewer

Length: 1668 bp
ATGGGTATTGATCTTTGGCTGGAACCATACTACCCAAATAGGTTTGCACGGCAGTTTGGTTTTGACCAGGGAGTTCCATCAAACAAGTTATTATTTACTGTTGAGAAAAGAAGACAGTGTTGCATTGAAGATCTAGCAAAAGCACAGCATATACTTCTTAGAGCTAATACCAGTGCCCGTTTTTACATCCCACGTTCTACTTATAATGGATCTTGCACTTGGATGTATTGTAGATGGTGGATGAGGGCATGCGCTCCATATTTGGGTCGATCCGTGTCTGCTGTTTATTTGACTCTAAAGAAGCAACCTTTAGAGAAAAGAAGTAATGTCTTTGTGATAAAATCTTTGAGAGAAGTCTCTTACAGTTTGCGAAGAGAAGTTTTGCTGACAGATGTTGAGGCAACAGATTCGACCACCCATTTTAGGAAATCTTCCTCTGTAGAAGACAACAAGAGGAAGAGGGATTGTTCCAGATCTGATAATTCAGGGGGAAGAAACCTCAAGCAAGCCCATAAAGATGAGCGTAGTGACTTTGATGACGTTATTGCCGATAATGCTAACTTTCTTCAGAAGAGACATACTCCAGATAAAGGCAATAGTTCTTATGGTGGCAGAACCACTGTTGCGAAAGAACAGAGAATACAATCTCGAGACCAAAAGTCGACATCCAGAGGAACAGAGTCAAATTCAAAAGAAGCACGACCGCAGACCAGAGAGACAGTGTTAAAGTTGAAGGAACCAGAGCCAAAGCCAAAGCCAAGACAGAGAGAATCAGAGCTAAAAGAGAAGCACCCAGAGAAAAGGGTTGCAGTTCCAGAGCCACAGTTAAATGAGCCAGAGCTGAAATCATTATCTGGCGGTAATTCCAACTCTCCTAGCAAAGCCACAGAGCTTATTGGTTCAGATACTTGCTCATCCCAGAGTTTTTGTGAAGAAATCATCCCATTGATTGAGAGCCCGTTGTCTATATATCAGATCTTTAGCCCAACGATAACTGAGCATCGACGAGATTTCATCATTACTGAGATGCAAGACATTTTAAGTAAGCTGAGTTTTTCTGGATCTCCTAAAGAAGTACTTGTTCACCGTCAGGATATTAACTCTACAATTGCCCTTTTGACATCTGTGATGGAAACTCTTGAGTCTAGCAGAAATGGATTTGATTGGTTTGCCAAGACAGTTGAGCAGATTTTTGAACTAGCATCTCAACTTTTGCAAGGAATGAGCACCATTGAGAAAGGTGCTGAAATTGATGCTGATCTTATCCAAGACTTAGCTCGTCACCAAGAAGAGTGCCAAACTGCTAAGAAGACTCTTAATATGCAACTGTCAGACTTCAAAAAACAACTTGCAGATTTAGAAGCCACAGAGAAGAGTGTTAATGAAGCAGAGAAGCAGGCTCATGAGGCAATGGAACGAGCTAAACAACTAAGAGCTACTTTTGAAGCTGCAAGAGGATCTCTTGAAGAGAAGATCAATGATTTGAAAAAATCTTCTGAAGAGGAAGACCAGTTAGATGAAGAGCTGCATCAAAAAAGAATTCAAGCCCATGAAGATAAACTTCCAGAATTTGAGCATGTGAAAGCCTCCTTCAAATCTCAGCAAGAGCAGTTTGCCAACTTAATTAATTCCTTGACATCATTTGGTGCTCAACCCAAACCCAAGTAG

Protein Analysis

555

Amino Acids

63.43

Weight (kDa)

7.58

Isoelectric Point (pI)

61.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 7 - 81 4.4e-08 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28912 FvH4_3g31370 FvH4_3g31371 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_6g28991 FvH4_7g16740 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610
malus_domestica MD10G1126100.v1.1 MD10G1312700.v1.1
rosa_chinensis RchiOBHm_Chr6g0276621
rosa_laevigata RLG00000023327 RLG00000023328
rosa_multiflora Rmu_co8195922.1_g000001 Rmu_co8303105.1_g000001 Rmu_co8385689.1_g000001 Rmu_sc0000385.1_g000010 Rmu_sc0000594.1_g000002 Rmu_sc0000857.1_g000010 Rmu_sc0001016.1_g000053 Rmu_sc0001468.1_g000028 Rmu_sc0001548.1_g000047 Rmu_sc0001548.1_g000048 Rmu_sc0002281.1_g000026 Rmu_sc0002281.1_g000027 Rmu_sc0002281.1_g000028 Rmu_sc0002478.1_g000001 Rmu_sc0002513.1_g000014 Rmu_sc0002662.1_g000017 Rmu_sc0003936.1_g000040 Rmu_sc0004084.1_g000047 Rmu_sc0005529.1_g000005 Rmu_sc0005529.1_g000006 Rmu_sc0005728.1_g000010 Rmu_sc0005747.1_g000017 Rmu_sc0005747.1_g000018 Rmu_sc0006385.1_g000001 Rmu_sc0006550.1_g000019 Rmu_sc0006803.1_g000033 Rmu_sc0007102.1_g000011 Rmu_sc0011018.1_g000001 Rmu_sc0011452.1_g000014 Rmu_sc0011805.1_g000001 Rmu_sc0011805.1_g000002 Rmu_sc0012566.1_g000001 Rmu_sc0013807.1_g000007 Rmu_sc0015817.1_g000001 Rmu_sc0015930.1_g000011 Rmu_sc0018873.1_g000003 Rmu_sc0020929.1_g000001
rosa_roxburghii Rroxscaffold_2G00081310 Rroxscaffold_2G00111790 Rroxscaffold_5G00349530 Rroxscaffold_5G00356270 Rroxscaffold_5G00356280 Rroxscaffold_5G00356290
rosa_rugosa Rorug01G0056400 Rorug01G0056500 Rorug02G0247000 Rorug03G0271700 Rorug04G0157900 Rorug05G0221700 Rorug05G0251400 Rorug05G0251400 Rorug06G0087300 Rorug07G0221900 Rorug07G0221900
rosa_samantha Rh1AG073200 Rh1AG073300 Rh1AG124600 Rh1AG124700 Rh1BG091900 Rh1CG031600 Rh1CG072100 Rh1CG072200 Rh1CG118800 Rh1CG276600 Rh1CG276700 Rh1DG077300 Rh2BG192000 Rh2BG330900 Rh2BG331000 Rh2BG517700 Rh2BG517800 Rh2CG188300 Rh2DG120800 Rh2DG323900 Rh2DG420700 Rh2DG433000 Rh2DG529400 Rh2DG529500 Rh3DG358100 Rh3DG358200 Rh4BG129700 Rh4BG129800 Rh4BG129900 Rh4BG166400 Rh4CG032000 Rh4DG024800 Rh4DG039500 Rh5AG223400 Rh5BG244500 Rh5BG244600 Rh5BG244700 Rh5CG363000 Rh5CG366900 Rh6AG117500 Rh6AG188600 Rh6AG188700 Rh6AG188800 Rh6BG111400 Rh6BG140800 Rh6BG178800 Rh6DG124100 Rh6DG210000 Rh7AG441200 Rh7BG296800 Rh7BG413900 Rh7BG414000 Rh7BG452400 Rh7DG234000 Rh7DG234100
rosa_wichuraiana Rw2G019410 Rw5G042140 Rw7G019590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AccI GTMKAC 1 cut(s) 662
AciI CCGC 2 cut(s) 704, 858
AclWI GGATC 4 cut(s) 217, 264, 1069, 1465
AcsI RAATTY 3 cut(s) 685, 1539, 1568
AcuI CTGAAG 2 cut(s) 551, 1518
AfaI GTAC 1 cut(s) 1077
AfiI CCNNNNNNNGG 3 cut(s) 263, 423, 590
AhdI GACNNNNNGTC 1 cut(s) 658
AjnI CCWGG 1 cut(s) 66
Alw21I GWGCWC 2 cut(s) 1229, 1651
Alw26I GTCTC 4 cut(s) 361, 568, 645, 710
AlwI GGATC 4 cut(s) 217, 264, 1069, 1465
Ama87I CYCGRG 1 cut(s) 648
ApeKI GCWGC 2 cut(s) 1448, 1525
ApoI RAATTY 3 cut(s) 685, 1539, 1568
AseI ATTAAT 1 cut(s) 1626
AspLEI GCGC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 1076, 1274
AvaI CYCGRG 1 cut(s) 648
BaeGI GKGCMC 1 cut(s) 178
BanII GRGCYC 1 cut(s) 959
BbsI GAAGAC 4 cut(s) 118, 450, 1316, 1512
Bbv12I GWGCWC 2 cut(s) 1229, 1651
BbvI GCAGC 2 cut(s) 1435, 1512
BccI CCATC 3 cut(s) 85, 228, 1123
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciT130I CCWGG 1 cut(s) 68
BcoDI GTCTC 4 cut(s) 361, 568, 645, 710
BfaI CTAG 4 cut(s) 134, 876, 1146, 1199
BfmI CTRYAG 1 cut(s) 438
BglII AGATCT 3 cut(s) 130, 473, 975
BisI GCNGC 2 cut(s) 1449, 1526
BlsI GCNGC 2 cut(s) 1450, 1527
BmcAI AGTACT 1 cut(s) 1077
Bme1390I CCNGG 1 cut(s) 68
BmeRI GACNNNNNGTC 1 cut(s) 658
BmeT110I CYCGRG 1 cut(s) 648
BmiI GGNNCC 2 cut(s) 24, 738
BmrFI CCNGG 1 cut(s) 68
BmsI GCATC 5 cut(s) 1009, 1017, 1211, 1243, 1537
BpiI GAAGAC 4 cut(s) 118, 450, 1316, 1512
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BpmI CTGGAG 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 485, 1160
BsaBI GATNNNNATC 1 cut(s) 1257
BsaI GGTCTC 1 cut(s) 645
BsaJI CCNNGG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 423, 590
Bse1I ACTGG 2 cut(s) 171, 1510
Bse3DI GCAATG 1 cut(s) 1416
Bse8I GATNNNNATC 1 cut(s) 1257
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 6 cut(s) 186, 228, 246, 665, 914, 939
BseJI GATNNNNATC 1 cut(s) 1257
BseLI CCNNNNNNNGG 3 cut(s) 263, 423, 590
BseMI GCAATG 1 cut(s) 1416
BseMII CTCAG 4 cut(s) 987, 1014, 1040, 1613
BseNI ACTGG 2 cut(s) 171, 1510
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 2 cut(s) 1435, 1512
Bsh1285I CGRYCG 1 cut(s) 704
BsiEI CGRYCG 1 cut(s) 704
BsiHKAI GWGCWC 2 cut(s) 1229, 1651
BsiHKCI CYCGRG 1 cut(s) 648
BslI CCNNNNNNNGG 3 cut(s) 263, 423, 590
BsmAI GTCTC 4 cut(s) 361, 568, 645, 710
Bso31I GGTCTC 1 cut(s) 645
BsoBI CYCGRG 1 cut(s) 648
Bsp1286I GDGCHC 4 cut(s) 178, 959, 1229, 1651
BspACI CCGC 2 cut(s) 704, 858
BspCNI CTCAG 4 cut(s) 988, 1015, 1041, 1612
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 2 cut(s) 24, 738
BspPI GGATC 4 cut(s) 217, 264, 1069, 1465
BspQI GCTCTTC 1 cut(s) 1515
BspTNI GGTCTC 1 cut(s) 645
BsrDI GCAATG 1 cut(s) 1416
BsrI ACTGG 2 cut(s) 171, 1510
BssECI CCNNGG 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 9 cut(s) 100, 117, 365, 622, 721, 825, 1088, 1180, 1329
Bst6I CTCTTC 8 cut(s) 367, 452, 566, 1284, 1367, 1461, 1494, 1515
BstC8I GCNNGC 3 cut(s) 250, 507, 1398
BstDEI CTNAG 8 cut(s) 158, 996, 1023, 1049, 1273, 1305, 1430, 1599
BstF5I GGATG 6 cut(s) 186, 228, 246, 665, 914, 939
BstHHI GCGC 1 cut(s) 254
BstMAI GTCTC 4 cut(s) 361, 568, 645, 710
BstMCI CGRYCG 1 cut(s) 704
BstMWI GCNNNNNNNGC 2 cut(s) 554, 1406
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 2 cut(s) 252, 1580
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 1435, 1512
BstV2I GAAGAC 4 cut(s) 118, 450, 1316, 1512
BstX2I RGATCY 6 cut(s) 130, 209, 473, 975, 1061, 1457
BstYI RGATCY 6 cut(s) 130, 209, 473, 975, 1061, 1457
BtsCI GGATG 6 cut(s) 186, 228, 246, 665, 914, 939
BtsIMutI CAGTG 4 cut(s) 122, 178, 618, 726
Cac8I GCNNGC 3 cut(s) 250, 507, 1398
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 1076
CviAII CATG 4 cut(s) 249, 1403, 1550, 1577
CviQI GTAC 1 cut(s) 1076
DdeI CTNAG 8 cut(s) 158, 996, 1023, 1049, 1273, 1305, 1430, 1599
DriI GACNNNNNGTC 1 cut(s) 658
Eam1104I CTCTTC 8 cut(s) 367, 452, 566, 1284, 1367, 1461, 1494, 1515
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 8 cut(s) 367, 452, 566, 1284, 1367, 1461, 1494, 1515
Eco24I GRGCYC 1 cut(s) 959
Eco31I GGTCTC 1 cut(s) 645
Eco57I CTGAAG 2 cut(s) 551, 1518
Eco88I CYCGRG 1 cut(s) 648
EcoRI GAATTC 1 cut(s) 1539
EcoRII CCWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 959
FaeI CATG 4 cut(s) 252, 1406, 1553, 1580
FalI AAGNNNNNCTT 6 cut(s) 1062, 1094, 1423, 1455, 1545, 1577
FatI CATG 4 cut(s) 248, 1402, 1549, 1576
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 2 cut(s) 1449, 1526
FokI GGATG 6 cut(s) 173, 235, 253, 652, 901, 926
FriOI GRGCYC 1 cut(s) 959
Fsp4HI GCNGC 2 cut(s) 1449, 1526
FspBI CTAG 4 cut(s) 134, 876, 1146, 1199
GlaI GCGC 1 cut(s) 253
GluI GCNGC 2 cut(s) 1449, 1526
GsuI CTGGAG 1 cut(s) 567
HhaI GCGC 1 cut(s) 254
Hin1II CATG 4 cut(s) 252, 1406, 1553, 1580
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HinfI GANTC 6 cut(s) 292, 407, 680, 770, 1142, 1312
HphI GGTGA 2 cut(s) 1076, 1274
Hpy166II GTNNAC 2 cut(s) 663, 1084
Hpy188I TCNGA 7 cut(s) 478, 570, 775, 904, 975, 1333, 1498
Hpy8I GTNNAC 2 cut(s) 663, 1084
Hpy99I CGWCG 1 cut(s) 1008
HpyAV CCTTC 2 cut(s) 727, 1600
HpyCH4III ACNGT 9 cut(s) 100, 117, 365, 622, 721, 825, 1088, 1180, 1329
HpyCH4IV ACGT 2 cut(s) 193, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 554, 1406
HpyF3I CTNAG 8 cut(s) 158, 996, 1023, 1049, 1273, 1305, 1430, 1599
HpySE526I ACGT 2 cut(s) 193, 540
Hsp92II CATG 4 cut(s) 252, 1406, 1553, 1580
HspAI GCGC 1 cut(s) 252
LguI GCTCTTC 1 cut(s) 1515
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 2 cut(s) 1435, 1512
LweI GCATC 5 cut(s) 1009, 1017, 1211, 1243, 1537
MaeI CTAG 4 cut(s) 134, 876, 1146, 1199
MaeII ACGT 2 cut(s) 193, 540
MaeIII GTNAC 2 cut(s) 527, 1280
MfeI CAATTG 1 cut(s) 1107
MflI RGATCY 6 cut(s) 130, 209, 473, 975, 1061, 1457
MhlI GDGCHC 4 cut(s) 178, 959, 1229, 1651
MluCI AATT 9 cut(s) 481, 685, 862, 1107, 1248, 1539, 1568, 1623, 1627
MlyI GAGTC 4 cut(s) 286, 689, 1151, 1306
MmeI TCCRAC 1 cut(s) 891
MseI TTAA 8 cut(s) 725, 827, 1040, 1098, 1317, 1380, 1622, 1626
MslI CAYNNNNRTG 2 cut(s) 221, 516
MspR9I CCNGG 1 cut(s) 68
MunI CAATTG 1 cut(s) 1107
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 2 cut(s) 554, 1406
NlaIII CATG 4 cut(s) 252, 1406, 1553, 1580
NlaIV GGNNCC 2 cut(s) 24, 738
NmuCI GTSAC 2 cut(s) 527, 1280
NspI RCATGY 2 cut(s) 252, 1580
PacI TTAATTAA 1 cut(s) 1626
PaeI GCATGC 1 cut(s) 252
PaeR7I CTCGAG 1 cut(s) 648
PagI TCATGA 1 cut(s) 1402
PciSI GCTCTTC 1 cut(s) 1515
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PfeI GAWTC 2 cut(s) 407, 770
PkrI GCNGC 2 cut(s) 1450, 1527
PleI GAGTC 4 cut(s) 286, 688, 1150, 1306
PpsI GAGTC 4 cut(s) 286, 688, 1150, 1306
PshBI ATTAAT 1 cut(s) 1626
PsiI TTATAA 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 24, 738
PsrI GAACNNNNNNTAC 2 cut(s) 624, 656
PsuI RGATCY 6 cut(s) 130, 209, 473, 975, 1061, 1457
RsaI GTAC 1 cut(s) 1077
RsaNI GTAC 1 cut(s) 1076
RseI CAYNNNNRTG 2 cut(s) 221, 516
SalI GTCGAC 1 cut(s) 661
SapI GCTCTTC 1 cut(s) 1515
SaqAI TTAA 8 cut(s) 725, 827, 1040, 1098, 1317, 1380, 1622, 1626
SatI GCNGC 2 cut(s) 1449, 1526
ScaI AGTACT 1 cut(s) 1077
SchI GAGTC 4 cut(s) 286, 689, 1151, 1306
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 4 cut(s) 178, 959, 1229, 1651
SfaNI GCATC 5 cut(s) 1009, 1017, 1211, 1243, 1537
SfcI CTRYAG 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 648
SlaI CTCGAG 1 cut(s) 648
SmiMI CAYNNNNRTG 2 cut(s) 221, 516
SmlI CTYRAG 3 cut(s) 500, 648, 1139
SmoI CTYRAG 3 cut(s) 500, 648, 1139
SphI GCATGC 1 cut(s) 252
Sse9I AATT 9 cut(s) 481, 685, 862, 1107, 1248, 1539, 1568, 1623, 1627
SsiI CCGC 2 cut(s) 704, 858
SspMI CTAG 4 cut(s) 134, 876, 1146, 1199
StyD4I CCNGG 1 cut(s) 66
TaaI ACNGT 9 cut(s) 100, 117, 365, 622, 721, 825, 1088, 1180, 1329
TaiI ACGT 2 cut(s) 196, 543
TaqI TCGA 5 cut(s) 268, 410, 649, 662, 1003
TasI AATT 9 cut(s) 481, 685, 862, 1107, 1248, 1539, 1568, 1623, 1627
TatI WGTACW 1 cut(s) 1075
TfiI GAWTC 2 cut(s) 407, 770
Tru1I TTAA 8 cut(s) 725, 827, 1040, 1098, 1317, 1380, 1622, 1626
Tru9I TTAA 8 cut(s) 725, 827, 1040, 1098, 1317, 1380, 1622, 1626
TscAI CASTG 4 cut(s) 122, 178, 625, 726
TseFI GTSAC 2 cut(s) 527, 1280
TseI GCWGC 2 cut(s) 1448, 1525
Tsp45I GTSAC 2 cut(s) 527, 1280
TspDTI ATGAA 4 cut(s) 1003, 1398, 1533, 1566
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 4 cut(s) 122, 178, 625, 726
VspI ATTAAT 1 cut(s) 1626
XapI RAATTY 3 cut(s) 685, 1539, 1568
XceI RCATGY 2 cut(s) 252, 1580
XhoI CTCGAG 1 cut(s) 648
XmiI GTMKAC 1 cut(s) 662
XspI CTAG 4 cut(s) 134, 876, 1146, 1199
ZrmI AGTACT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.