Rh4BG166400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000253
Physical Location & Seq
Reverse (-)
30033716 .. 30034294
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG166400.1

Sequence Viewer

Length: 579 bp
ATGGAAGCTCAAGATCAAGTCATTGAGAATGAACCAGTTGTGCCAACTATTTATTTGAGCGAAACATCTTCAAACCAAGCTTTGCCGAAATTGTCGAACCTAGAGAGTTCGATATCTAACTTGCTTGGTTCAGAATTGCCATCTATCAAGAATGATTACCTCATTGCTCAGGTTGTGGACATTTATGATAAAGTTTCTTCTAGTCAAACTCCAAAGGAATTGTTAACCTATGTTCCGAACATCACATCAAGCCTTTATATGCTCCACCCTACAATTACACCCCTGAAAGCTGGAAAGTTCCAACTTAAAGCATTTATGACTACTATTAAGAAGATGCTGGATGGGGCGGATCAGACATTGGCTACTACTGGGATCCTTGAGCAGAATACTACCATGGATATTGAACTCGAGGAGTGTATGAAATCCAAGGTGACTTTTGAGTCTCAACTCGCAAGATCTTCCAACGAACTACAAGCCTTGAAAACTACGAAATTTGACATCATAGCAGATGAACAGAGAGTTCAAAATAGGCGATCAGAATATGATGCTAAGAGGCAATCCCTTGAAGACACTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.53

Weight (kDa)

4.76

Isoelectric Point (pI)

59.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28912 FvH4_3g31370 FvH4_3g31371 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_4g10340 FvH4_6g28991 FvH4_7g16740 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610 FvH4_7g18610
malus_domestica MD10G1126100.v1.1 MD10G1312700.v1.1
rosa_chinensis RchiOBHm_Chr6g0276621
rosa_laevigata RLG00000023327 RLG00000023328
rosa_multiflora Rmu_co8195922.1_g000001 Rmu_co8303105.1_g000001 Rmu_co8385689.1_g000001 Rmu_sc0000385.1_g000010 Rmu_sc0000594.1_g000002 Rmu_sc0000857.1_g000010 Rmu_sc0001016.1_g000053 Rmu_sc0001468.1_g000028 Rmu_sc0001548.1_g000047 Rmu_sc0001548.1_g000048 Rmu_sc0002281.1_g000026 Rmu_sc0002281.1_g000027 Rmu_sc0002281.1_g000028 Rmu_sc0002478.1_g000001 Rmu_sc0002513.1_g000014 Rmu_sc0002662.1_g000017 Rmu_sc0003936.1_g000040 Rmu_sc0004084.1_g000047 Rmu_sc0005529.1_g000005 Rmu_sc0005529.1_g000006 Rmu_sc0005728.1_g000010 Rmu_sc0005747.1_g000017 Rmu_sc0005747.1_g000018 Rmu_sc0006385.1_g000001 Rmu_sc0006550.1_g000019 Rmu_sc0006803.1_g000033 Rmu_sc0007102.1_g000011 Rmu_sc0011018.1_g000001 Rmu_sc0011452.1_g000014 Rmu_sc0011805.1_g000001 Rmu_sc0011805.1_g000002 Rmu_sc0012566.1_g000001 Rmu_sc0013807.1_g000007 Rmu_sc0015817.1_g000001 Rmu_sc0015930.1_g000011 Rmu_sc0018873.1_g000003 Rmu_sc0020929.1_g000001
rosa_roxburghii Rroxscaffold_2G00081310 Rroxscaffold_2G00111790 Rroxscaffold_5G00349530 Rroxscaffold_5G00356270 Rroxscaffold_5G00356280 Rroxscaffold_5G00356290
rosa_rugosa Rorug01G0056400 Rorug01G0056500 Rorug02G0247000 Rorug03G0271700 Rorug04G0157900 Rorug05G0221700 Rorug05G0251400 Rorug05G0251400 Rorug06G0087300 Rorug07G0221900 Rorug07G0221900
rosa_samantha Rh1AG073200 Rh1AG073300 Rh1AG124600 Rh1AG124700 Rh1BG091900 Rh1CG031600 Rh1CG072100 Rh1CG072200 Rh1CG118800 Rh1CG276600 Rh1CG276700 Rh1DG077300 Rh2BG192000 Rh2BG330900 Rh2BG331000 Rh2BG517700 Rh2BG517800 Rh2CG188300 Rh2DG120800 Rh2DG323900 Rh2DG420700 Rh2DG433000 Rh2DG529400 Rh2DG529500 Rh3DG358100 Rh3DG358200 Rh4BG129700 Rh4BG129800 Rh4BG129900 Rh4BG166400 Rh4CG032000 Rh4DG024800 Rh4DG039500 Rh5AG223400 Rh5BG244500 Rh5BG244600 Rh5BG244700 Rh5CG363000 Rh5CG366900 Rh6AG117500 Rh6AG188600 Rh6AG188700 Rh6AG188800 Rh6BG111400 Rh6BG140800 Rh6BG178800 Rh6DG124100 Rh6DG210000 Rh7AG441200 Rh7BG296800 Rh7BG413900 Rh7BG414000 Rh7BG452400 Rh7DG234000 Rh7DG234100
rosa_wichuraiana Rw2G019410 Rw5G042140 Rw7G019590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 439
AciI CCGC 1 cut(s) 347
AclWI GGATC 3 cut(s) 357, 367, 380
AcsI RAATTY 1 cut(s) 491
AgsI TTSAA 5 cut(s) 72, 404, 481, 524, 566
AluBI AGCT 3 cut(s) 8, 80, 290
AluI AGCT 3 cut(s) 8, 80, 290
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 3 cut(s) 357, 367, 380
Ama87I CYCGRG 1 cut(s) 407
ApoI RAATTY 1 cut(s) 491
AsuHPI GGTGA 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 407
BamHI GGATCC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 573
BccI CCATC 2 cut(s) 148, 335
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 2 cut(s) 101, 201
BfmI CTRYAG 1 cut(s) 575
BglII AGATCT 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 407
BmiI GGNNCC 1 cut(s) 374
BmrI ACTGGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 324, 535
BmuI ACTGGG 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 573
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 393, 426
Bse1I ACTGG 2 cut(s) 35, 373
Bse3DI GCAATG 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 393, 426
BseGI GGATG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 2 cut(s) 35, 373
BseRI GAGGAG 1 cut(s) 425
BsiHKCI CYCGRG 1 cut(s) 407
BsmAI GTCTC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 407
Bsp143I GATC 5 cut(s) 13, 349, 372, 455, 533
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 374
BspPI GGATC 3 cut(s) 357, 367, 380
BsrDI GCAATG 1 cut(s) 162
BsrI ACTGG 2 cut(s) 35, 373
BssECI CCNNGG 2 cut(s) 393, 426
BssMI GATC 5 cut(s) 13, 349, 372, 455, 533
BssT1I CCWWGG 2 cut(s) 393, 426
BstDEI CTNAG 2 cut(s) 168, 549
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 1 cut(s) 346
BstKTI GATC 5 cut(s) 16, 352, 375, 458, 536
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 5 cut(s) 13, 349, 372, 455, 533
BstSFI CTRYAG 1 cut(s) 575
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 2 cut(s) 372, 455
BstYI RGATCY 2 cut(s) 372, 455
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 1 cut(s) 346
CviAII CATG 1 cut(s) 394
CviJI RGCY 6 cut(s) 8, 80, 252, 290, 362, 476
CviKI_1 RGCY 6 cut(s) 8, 80, 252, 290, 362, 476
DdeI CTNAG 2 cut(s) 168, 549
DpnI GATC 5 cut(s) 15, 351, 374, 457, 535
DpnII GATC 5 cut(s) 13, 349, 372, 455, 533
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EciI GGCGGA 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 393, 426
Eco32I GATATC 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 407
EcoRV GATATC 1 cut(s) 114
EcoT14I CCWWGG 2 cut(s) 393, 426
ErhI CCWWGG 2 cut(s) 393, 426
FaeI CATG 1 cut(s) 397
FaiI YATR 9 cut(s) 186, 231, 258, 260, 317, 395, 419, 503, 543
FatI CATG 1 cut(s) 393
FokI GGATG 1 cut(s) 353
FspBI CTAG 2 cut(s) 101, 201
Hin1II CATG 1 cut(s) 397
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 1 cut(s) 440
HpaI GTTAAC 1 cut(s) 225
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 2 cut(s) 178, 225
Hpy188I TCNGA 4 cut(s) 133, 237, 354, 538
Hpy188III TCNNGA 2 cut(s) 11, 148
Hpy8I GTNNAC 2 cut(s) 178, 225
HpyF3I CTNAG 2 cut(s) 168, 549
Hsp92II CATG 1 cut(s) 397
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 5 cut(s) 13, 349, 372, 455, 533
LmnI GCTCC 1 cut(s) 267
LpnPI CCDG 6 cut(s) 48, 155, 276, 296, 323, 354
LweI GCATC 2 cut(s) 324, 535
MaeI CTAG 2 cut(s) 101, 201
MaeIII GTNAC 1 cut(s) 430
MalI GATC 5 cut(s) 15, 351, 374, 457, 535
MboI GATC 5 cut(s) 13, 349, 372, 455, 533
MboII GAAGA 5 cut(s) 60, 189, 343, 450, 578
MflI RGATCY 2 cut(s) 372, 455
MluCI AATT 5 cut(s) 89, 134, 218, 273, 491
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 325, 486
MnlI CCTC 3 cut(s) 170, 403, 546
MseI TTAA 3 cut(s) 224, 306, 327
NcoI CCATGG 1 cut(s) 393
NdeII GATC 5 cut(s) 13, 349, 372, 455, 533
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 1 cut(s) 430
PaeR7I CTCGAG 1 cut(s) 407
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 374
PspXI VCTCGAGB 1 cut(s) 407
PsuI RGATCY 2 cut(s) 372, 455
SaqAI TTAA 3 cut(s) 224, 306, 327
Sau3AI GATC 5 cut(s) 13, 349, 372, 455, 533
SchI GAGTC 1 cut(s) 449
SetI ASST 8 cut(s) 10, 82, 102, 162, 174, 230, 292, 432
SfaNI GCATC 2 cut(s) 324, 535
SfcI CTRYAG 1 cut(s) 575
Sfr274I CTCGAG 1 cut(s) 407
SlaI CTCGAG 1 cut(s) 407
SmlI CTYRAG 3 cut(s) 9, 377, 407
SmoI CTYRAG 3 cut(s) 9, 377, 407
Sse9I AATT 5 cut(s) 89, 134, 218, 273, 491
SsiI CCGC 1 cut(s) 347
SspMI CTAG 2 cut(s) 101, 201
StyI CCWWGG 2 cut(s) 393, 426
TaqI TCGA 3 cut(s) 95, 110, 408
TasI AATT 5 cut(s) 89, 134, 218, 273, 491
Tru1I TTAA 3 cut(s) 224, 306, 327
Tru9I TTAA 3 cut(s) 224, 306, 327
TseFI GTSAC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 3 cut(s) 45, 434, 525
XapI RAATTY 1 cut(s) 491
XhoI CTCGAG 1 cut(s) 407
XspI CTAG 2 cut(s) 101, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.