FvH4_3g27562

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000080
Physical Location & Seq
Forward (+)
20624612 .. 20626535
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27562.t1

Sequence Viewer

Length: 1431 bp
ATGTTACGTGATGAGGATAAGCTTAATAACCCTGATGATTATGATAAAATAGTTCAAGCTGAGATACCAGATCAAAATGATGAGCCTCAGTTGTACGATGTTGTATTGAGACACATGATGCATGGTCCATGTGGAACCCATAAGCCAAACTCCCCATGCATGAGACGTGGAAATTGCAAACGAGGTTCTCCAAAATCGTTTTTGATGAATACATTTCAAGGGAATGACTCTTATCCTACTTACTGCAGGCGTGATCTTCACTCAAGTTCAAATGTTGATAACAGGTGGGTAATTCCATACAACCCTTGGTTACTTGCGAAATATGACTGTCATATCAATGTCGAAATCTGTTCTAGCATTAAAAGTGTTAAGTATTTGTACAAGTATGTTTACAAAGGTCCAGATAAAGTAACGTTTGCAGTCCAACCTAAACGCGATGTGGATGAAATCAAAAATTTTGTTGATGCAAGATGGGAAGCCTCCAACATACGTATGCTTTCATCTTTAAGAAGATTGTTTGCCACCATCTTAGTTCATTGCGAGCCTCATGGCGTTCGAAATTTGTGGGATCAATATTACCAATTTATGATAGAAGATTATACTTCTTCAAGTTCTCCAAACAACGCGCATGCCACGAACAGACTTTTGCGAGACTTGAACGAATTGTTGGCCCAACATAGTAAATCTATCTCGGATTATGATTTGCCACAAATGACAGGAGATAATAACGAAGGATCAAAATTGCCAAGGCTAATACAAGATGAAGTTTCAGTTATCACTCCCCAACAAGATTACAACGCAGTTTATCATCTGAATCAAGATCAGACTTTTGCATACAACACTATAATATCTTCTATTGAGCGAAACGACAATGTCATGTTCTTTGTTGATGGTTCAGGAGGTACTGGAAAAACATACTTATATCGTGCATTGTTAGCAACGTTGAGAAATAAGAACCATATTGTACTTGCAACAGCAACCTCTGGAATATCAGCAACAATACTTCCTGGTGGAAGAACGGCACACTCAAGGTTTAAAATTCCACTAGATCCTGAAAAAACTCCTACATGCTCAATTAGTAAACAATCAGATCTAGCTGAGTTAATACGAACATCATCAGCTATTATATGTGATGAAGCACCAATGATGCATAAATGTGCGTTCGAGGCTCTGAATGAAACTTTGAAAGATATAACAGAAGTCAAATTACCATTTGGAGGTAAAGTAATTATCTTTGGCGGGGACTTCCGACAAATCCCACCAGTTGTTCCTAAAGGTACAAGGTCAGAAATGGTCAGTGCCAGTATGATAAATGCCTCATTTTGGAAAGATGTGAAAATCCTTCGTTTACGACAAAATATGAGATCCATCAACGATCCGCAGTTCTCAGACTTTTACTTAATGTTGGAAATGGAGAACAACCATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

477

Amino Acids

54.39

Weight (kDa)

6.92

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 271 - 458 1.2e-70 PIF1-like helicase
AAA_30 PF13604 271 - 435 1.1e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 435, 626
AciI CCGC 2 cut(s) 1239, 1379
AclI AACGTT 2 cut(s) 413, 941
AclWI GGATC 5 cut(s) 576, 742, 1043, 1359, 1370
AcsI RAATTY 3 cut(s) 454, 559, 1038
AfaI GTAC 5 cut(s) 95, 380, 904, 966, 1279
AfiI CCNNNNNNNGG 2 cut(s) 1217, 1323
AgsI TTSAA 6 cut(s) 56, 218, 270, 609, 658, 1186
AjiI CACGTC 1 cut(s) 167
AjnI CCWGG 1 cut(s) 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
AluBI AGCT 4 cut(s) 22, 59, 1097, 1121
AluI AGCT 4 cut(s) 22, 59, 1097, 1121
Alw26I GTCTC 3 cut(s) 103, 157, 645
AlwI GGATC 5 cut(s) 576, 742, 1043, 1359, 1370
AoxI GGCC 1 cut(s) 669
ApoI RAATTY 3 cut(s) 454, 559, 1038
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 3 cut(s) 125, 398, 670
AsuII TTCGAA 1 cut(s) 556
AvaII GGWCC 2 cut(s) 125, 398
BarI GAAGNNNNNNTAC 2 cut(s) 1191, 1223
BccI CCATC 4 cut(s) 465, 533, 884, 1376
BceAI ACGGC 1 cut(s) 1035
BciT130I CCWGG 1 cut(s) 1008
BcoDI GTCTC 3 cut(s) 103, 157, 645
BfaI CTAG 3 cut(s) 354, 1046, 1094
BfmI CTRYAG 1 cut(s) 244
BglII AGATCT 1 cut(s) 1090
Bme1390I CCNGG 1 cut(s) 1008
Bme18I GGWCC 2 cut(s) 125, 398
BmgBI CACGTC 1 cut(s) 167
BmgT120I GGNCC 3 cut(s) 125, 398, 670
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 1008
BmsI GCATC 3 cut(s) 108, 454, 1137
Bpu14I TTCGAA 1 cut(s) 556
BpuEI CTTGAG 2 cut(s) 247, 1012
BsaAI YACGTR 2 cut(s) 8, 491
BsaJI CCNNGG 2 cut(s) 305, 746
BsaXI ACNNNNNCTCC 2 cut(s) 1209, 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 1217, 1323
Bse1I ACTGG 3 cut(s) 910, 1262, 1302
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 1008
BseDI CCNNGG 2 cut(s) 305, 746
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 2 cut(s) 1217, 1323
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 4 cut(s) 51, 101, 1089, 1401
BseNI ACTGG 3 cut(s) 910, 1262, 1302
Bsh1236I CGCG 2 cut(s) 435, 626
BshFI GGCC 1 cut(s) 671
BslFI GGGAC 1 cut(s) 1256
BslI CCNNNNNNNGG 2 cut(s) 1217, 1323
BsmAI GTCTC 3 cut(s) 103, 157, 645
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 1256
BsnI GGCC 1 cut(s) 671
Bsp119I TTCGAA 1 cut(s) 556
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
BspACI CCGC 2 cut(s) 1239, 1379
BspANI GGCC 1 cut(s) 671
BspCNI CTCAG 4 cut(s) 52, 100, 1090, 1400
BspFNI CGCG 2 cut(s) 435, 626
BspLI GGNNCC 1 cut(s) 136
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 5 cut(s) 576, 742, 1043, 1359, 1370
BspT104I TTCGAA 1 cut(s) 556
BsrDI GCAATG 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 3 cut(s) 910, 1262, 1302
BssECI CCNNGG 2 cut(s) 305, 746
BssMI GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
BssT1I CCWWGG 2 cut(s) 305, 746
Bst2UI CCWGG 1 cut(s) 1008
Bst4CI ACNGT 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 378
BstBAI YACGTR 2 cut(s) 8, 491
BstBI TTCGAA 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 248, 542, 630
BstDEI CTNAG 5 cut(s) 60, 87, 529, 1098, 1387
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 2 cut(s) 435, 626
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 9 cut(s) 73, 256, 571, 737, 823, 1051, 1093, 1367, 1378
BstMAI GTCTC 3 cut(s) 103, 157, 645
BstMBI GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
BstMWI GCNNNNNNNGC 2 cut(s) 935, 1166
BstNI CCWGG 1 cut(s) 1008
BstNSI RCATGY 2 cut(s) 632, 1071
BstSCI CCNGG 1 cut(s) 1006
BstSFI CTRYAG 1 cut(s) 244
BstSNI TACGTA 1 cut(s) 491
BstUI CGCG 2 cut(s) 435, 626
BstX2I RGATCY 3 cut(s) 1048, 1090, 1364
BstYI RGATCY 3 cut(s) 1048, 1090, 1364
BsuRI GGCC 1 cut(s) 671
BtgZI GCGATG 1 cut(s) 450
BtrI CACGTC 1 cut(s) 167
BtsCI GGATG 1 cut(s) 448
BtsIMutI CAGTG 1 cut(s) 1303
Cac8I GCNNGC 3 cut(s) 248, 542, 630
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 3 cut(s) 125, 398, 670
Csp6I GTAC 5 cut(s) 94, 379, 903, 965, 1278
CviAII CATG 9 cut(s) 115, 122, 129, 156, 160, 548, 629, 877, 1068
CviQI GTAC 5 cut(s) 94, 379, 903, 965, 1278
DdeI CTNAG 5 cut(s) 60, 87, 529, 1098, 1387
DpnI GATC 9 cut(s) 72, 255, 570, 736, 822, 1050, 1092, 1366, 1377
DpnII GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
DraI TTTAAA 1 cut(s) 1036
Eco105I TACGTA 1 cut(s) 491
Eco130I CCWWGG 2 cut(s) 305, 746
Eco47I GGWCC 2 cut(s) 125, 398
EcoRII CCWGG 1 cut(s) 1006
EcoT14I CCWWGG 2 cut(s) 305, 746
EcoT22I ATGCAT 3 cut(s) 123, 161, 1152
ErhI CCWWGG 2 cut(s) 305, 746
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 9 cut(s) 118, 125, 132, 159, 163, 551, 632, 880, 1071
FaqI GGGAC 1 cut(s) 1256
FatI CATG 9 cut(s) 114, 121, 128, 155, 159, 547, 628, 876, 1067
FauI CCCGC 1 cut(s) 1232
FauNDI CATATG 1 cut(s) 1425
FokI GGATG 1 cut(s) 455
FspBI CTAG 3 cut(s) 354, 1046, 1094
GlaI GCGC 1 cut(s) 627
HaeIII GGCC 1 cut(s) 671
HhaI GCGC 1 cut(s) 628
Hin1II CATG 9 cut(s) 118, 125, 132, 159, 163, 551, 632, 880, 1071
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 2 cut(s) 227, 814
Hpy166II GTNNAC 3 cut(s) 391, 1082, 1349
Hpy188I TCNGA 8 cut(s) 694, 813, 825, 1090, 1173, 1250, 1288, 1390
Hpy188III TCNNGA 5 cut(s) 401, 818, 897, 984, 1052
Hpy8I GTNNAC 3 cut(s) 391, 1082, 1349
HpyAV CCTTC 2 cut(s) 725, 1352
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 5 cut(s) 7, 166, 413, 490, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 935, 1166
HpyF3I CTNAG 5 cut(s) 60, 87, 529, 1098, 1387
HpySE526I ACGT 5 cut(s) 7, 166, 413, 490, 941
Hsp92II CATG 9 cut(s) 118, 125, 132, 159, 163, 551, 632, 880, 1071
HspAI GCGC 1 cut(s) 626
Kzo9I GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
LweI GCATC 3 cut(s) 108, 454, 1137
MaeI CTAG 3 cut(s) 354, 1046, 1094
MaeII ACGT 5 cut(s) 7, 166, 413, 490, 941
MaeIII GTNAC 3 cut(s) 3, 309, 409
MalI GATC 9 cut(s) 72, 255, 570, 736, 822, 1050, 1092, 1366, 1377
MboI GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
MboII GAAGA 6 cut(s) 248, 522, 597, 605, 843, 1026
MflI RGATCY 3 cut(s) 1048, 1090, 1364
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 4 cut(s) 448, 507, 1273, 1386
Mph1103I ATGCAT 3 cut(s) 123, 161, 1152
MseI TTAA 7 cut(s) 24, 360, 369, 506, 1035, 1103, 1400
MslI CAYNNNNRTG 3 cut(s) 336, 491, 1155
MspR9I CCNGG 1 cut(s) 1008
MvaI CCWGG 1 cut(s) 1008
MvnI CGCG 2 cut(s) 435, 626
MwoI GCNNNNNNNGC 2 cut(s) 935, 1166
NdeI CATATG 1 cut(s) 1425
NdeII GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
NlaIII CATG 9 cut(s) 118, 125, 132, 159, 163, 551, 632, 880, 1071
NlaIV GGNNCC 1 cut(s) 136
NsiI ATGCAT 3 cut(s) 123, 161, 1152
NspI RCATGY 2 cut(s) 632, 1071
NspV TTCGAA 1 cut(s) 556
PaeI GCATGC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 814
PflFI GACNNNGTC 1 cut(s) 872
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Ppu21I YACGTR 2 cut(s) 8, 491
Psp1406I AACGTT 2 cut(s) 413, 941
Psp6I CCWGG 1 cut(s) 1006
PspGI CCWGG 1 cut(s) 1006
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 3 cut(s) 125, 398, 670
PstI CTGCAG 1 cut(s) 248
PsuI RGATCY 3 cut(s) 1048, 1090, 1364
PsyI GACNNNGTC 1 cut(s) 872
RsaI GTAC 5 cut(s) 95, 380, 904, 966, 1279
RsaNI GTAC 5 cut(s) 94, 379, 903, 965, 1278
RseI CAYNNNNRTG 3 cut(s) 336, 491, 1155
SaqAI TTAA 7 cut(s) 24, 360, 369, 506, 1035, 1103, 1400
Sau3AI GATC 9 cut(s) 70, 253, 568, 734, 820, 1048, 1090, 1364, 1375
Sau96I GGNCC 3 cut(s) 125, 398, 670
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 1008
SfaNI GCATC 3 cut(s) 108, 454, 1137
SfcI CTRYAG 1 cut(s) 244
SfuI TTCGAA 1 cut(s) 556
SinI GGWCC 2 cut(s) 125, 398
SmiMI CAYNNNNRTG 3 cut(s) 336, 491, 1155
SmlI CTYRAG 2 cut(s) 262, 1027
SmoI CTYRAG 2 cut(s) 262, 1027
SnaBI TACGTA 1 cut(s) 491
SphI GCATGC 1 cut(s) 632
SsiI CCGC 2 cut(s) 1239, 1379
SspI AATATT 1 cut(s) 575
SspMI CTAG 3 cut(s) 354, 1046, 1094
StyD4I CCNGG 1 cut(s) 1006
StyI CCWWGG 2 cut(s) 305, 746
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 5 cut(s) 10, 169, 416, 493, 944
TaqI TCGA 3 cut(s) 342, 556, 1164
TatI WGTACW 2 cut(s) 378, 964
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 7 cut(s) 24, 360, 369, 506, 1035, 1103, 1400
Tru9I TTAA 7 cut(s) 24, 360, 369, 506, 1035, 1103, 1400
TscAI CASTG 1 cut(s) 1303
TspDTI ATGAA 7 cut(s) 221, 459, 489, 524, 777, 1149, 1191
TspRI CASTG 1 cut(s) 1303
Tth111I GACNNNGTC 1 cut(s) 872
VpaK11BI GGWCC 2 cut(s) 125, 398
XapI RAATTY 3 cut(s) 454, 559, 1038
XceI RCATGY 2 cut(s) 632, 1071
XcmI CCANNNNNNNNNTGG 1 cut(s) 303
XspI CTAG 3 cut(s) 354, 1046, 1094
Zsp2I ATGCAT 3 cut(s) 123, 161, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.