Rmu_sc0006729.1_g000003

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006729.1
OG0000080
Physical Location & Seq
Reverse (-)
21793 .. 23526
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006729.1_g000003.1.cds

Sequence Viewer

Length: 828 bp
atgaaatttagtgttggatttggtgggcttacgatgagggactggactagagagatgatctcgactaggtcttggaagaggacgcggtcgatgttgccgttggcggcggaggaggacaaagttaagtggtggtggtggtggtcagtcaagtccccgaatgaccatataattgttgttgggaacattagagattttgaccttcctgaaatgacaagggagccagaagaaaattcaagattgccgaggtgtattgaagacaaactttccatacatatctcgcaagaagacatcgatgcaattgaccaccttaatgaagaccaaaggaccgcctttaacacgatcatggatgcagttcagataaaaagaagtgcattattttttgttgatggtcctggtggaactgggaagacatatttataccgtgcattgctagcaaacttacggagaatgaatcatatagtcttagccacagcatcatctagaatagctgcaaatatattgcccggcgggaggacagctcactctagatttaagattccactcaatgtagatgcatcatctatgtgttccataagtaaacagtctgctctggcacaactgataccagattcgaaagcaattatttgggataaggcacctatgacacatcgacatgtctttgaagcacttgatcgaacttttcgagacataatggatgtcgacttaccattcggtggaaagataatgattttttggggagattttggtcaagtcctctatgttattcctaaaggaacgaagtctgaactcattcaagcctccattgtgaaatcaacattttgggcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

275

Amino Acids

31.38

Weight (kDa)

9.05

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 634
AccB7I CCANNNNNTGG 1 cut(s) 713
AccI GTMKAC 1 cut(s) 699
AccII CGCG 1 cut(s) 85
AciI CCGC 5 cut(s) 85, 104, 107, 327, 507
AcsI RAATTY 2 cut(s) 5, 229
AfiI CCNNNNNNNGG 2 cut(s) 510, 713
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 4 cut(s) 234, 254, 662, 794
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 2 cut(s) 488, 518
AluI AGCT 2 cut(s) 488, 518
Alw26I GTCTC 1 cut(s) 678
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 2 cut(s) 5, 229
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
Asp700I GAANNNNTTC 1 cut(s) 789
AspS9I GGNCC 2 cut(s) 324, 389
AsuC2I CCSGG 1 cut(s) 504
AsuII TTCGAA 1 cut(s) 611
AsuNHI GCTAGC 1 cut(s) 430
AvaII GGWCC 2 cut(s) 324, 389
BanI GGYRCC 1 cut(s) 634
BbsI GAAGAC 4 cut(s) 261, 291, 321, 413
BbvI GCAGC 1 cut(s) 475
BccI CCATC 1 cut(s) 380
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 393
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 1 cut(s) 678
BfaI CTAG 5 cut(s) 48, 66, 431, 480, 525
BisI GCNGC 2 cut(s) 105, 489
BlsI GCNGC 2 cut(s) 106, 490
Bme1390I CCNGG 2 cut(s) 393, 504
Bme18I GGWCC 2 cut(s) 324, 389
BmgT120I GGNCC 2 cut(s) 324, 389
BmiI GGNNCC 2 cut(s) 219, 636
BmrFI CCNGG 2 cut(s) 393, 504
BmrI ACTGGG 1 cut(s) 411
BmsI GCATC 5 cut(s) 283, 337, 482, 541, 563
BmtI GCTAGC 1 cut(s) 434
BmuI ACTGGG 1 cut(s) 411
BpiI GAAGAC 4 cut(s) 261, 291, 321, 413
BplI GAGNNNNNCTC 4 cut(s) 44, 76, 502, 534
Bpu14I TTCGAA 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 242
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 713
Bse1I ACTGG 2 cut(s) 47, 406
Bse3DI GCAATG 1 cut(s) 425
BseBI CCWGG 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 2 cut(s) 352, 700
BseLI CCNNNNNNNGG 2 cut(s) 510, 713
BseMI GCAATG 1 cut(s) 425
BseNI ACTGG 2 cut(s) 47, 406
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 475
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 634
BshVI ATCGAT 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 2 cut(s) 53, 136
BslI CCNNNNNNNGG 2 cut(s) 510, 713
BsmAI GTCTC 1 cut(s) 678
BsmFI GGGAC 2 cut(s) 53, 136
Bsp119I TTCGAA 1 cut(s) 611
Bsp143I GATC 3 cut(s) 57, 339, 670
BspACI CCGC 5 cut(s) 85, 104, 107, 327, 507
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 2 cut(s) 219, 636
BspOI GCTAGC 1 cut(s) 434
BspT104I TTCGAA 1 cut(s) 611
BspT107I GGYRCC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 425
BsrI ACTGG 2 cut(s) 47, 406
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 3 cut(s) 57, 339, 670
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 422, 582
Bst6I CTCTTC 1 cut(s) 71
BstBI TTCGAA 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 463
BstF5I GGATG 2 cut(s) 352, 700
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 3 cut(s) 60, 342, 673
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 3 cut(s) 57, 339, 670
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 391, 502
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 475
BstV2I GAAGAC 4 cut(s) 261, 291, 321, 413
Bsu15I ATCGAT 1 cut(s) 291
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 2 cut(s) 352, 700
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 324, 389
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 1 cut(s) 91
CviAII CATG 2 cut(s) 343, 653
CviJI RGCY 6 cut(s) 28, 220, 467, 488, 518, 797
CviKI_1 RGCY 6 cut(s) 28, 220, 467, 488, 518, 797
DdeI CTNAG 1 cut(s) 463
DpnI GATC 3 cut(s) 59, 341, 672
DpnII GATC 3 cut(s) 57, 339, 670
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
EciI GGCGGA 1 cut(s) 122
Eco47I GGWCC 2 cut(s) 324, 389
EcoRII CCWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 556
FaeI CATG 2 cut(s) 346, 656
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 2 cut(s) 53, 136
FatI CATG 2 cut(s) 342, 652
FauI CCCGC 1 cut(s) 500
FblI GTMKAC 1 cut(s) 699
Fnu4HI GCNGC 2 cut(s) 105, 489
FokI GGATG 2 cut(s) 359, 707
Fsp4HI GCNGC 2 cut(s) 105, 489
FspBI CTAG 5 cut(s) 48, 66, 431, 480, 525
GluI GCNGC 2 cut(s) 105, 489
HapII CCGG 1 cut(s) 504
HgaI GACGC 1 cut(s) 91
Hin1II CATG 2 cut(s) 346, 656
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HinfI GANTC 3 cut(s) 451, 535, 608
HpaII CCGG 1 cut(s) 504
Hpy166II GTNNAC 2 cut(s) 578, 700
Hpy188I TCNGA 2 cut(s) 357, 784
Hpy188III TCNNGA 6 cut(s) 61, 203, 234, 480, 525, 683
Hpy8I GTNNAC 2 cut(s) 578, 700
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 422, 582
HpyCH4V TGCA 6 cut(s) 296, 350, 371, 425, 491, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 1 cut(s) 463
Hsp92II CATG 2 cut(s) 346, 656
Kzo9I GATC 3 cut(s) 57, 339, 670
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 9 cut(s) 28, 216, 234, 378, 387, 405, 517, 575, 618
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 5 cut(s) 283, 337, 482, 541, 563
MaeI CTAG 5 cut(s) 48, 66, 431, 480, 525
MalI GATC 3 cut(s) 59, 341, 672
MboI GATC 3 cut(s) 57, 339, 670
MboII GAAGA 6 cut(s) 88, 236, 266, 296, 326, 418
MfeI CAATTG 1 cut(s) 297
MluCI AATT 5 cut(s) 5, 168, 229, 297, 618
MnlI CCTC 8 cut(s) 30, 72, 103, 106, 237, 504, 764, 808
Mph1103I ATGCAT 1 cut(s) 556
MroXI GAANNNNTTC 1 cut(s) 789
MseI TTAA 4 cut(s) 123, 309, 333, 531
MslI CAYNNNNRTG 4 cut(s) 309, 341, 562, 651
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 2 cut(s) 393, 504
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 393
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 1 cut(s) 431
NciI CCSGG 1 cut(s) 504
NdeII GATC 3 cut(s) 57, 339, 670
NheI GCTAGC 1 cut(s) 430
NlaIII CATG 2 cut(s) 346, 656
NlaIV GGNNCC 2 cut(s) 219, 636
NmeAIII GCCGAG 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 611
PciI ACATGT 1 cut(s) 652
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 679
PdmI GAANNNNTTC 1 cut(s) 789
PfeI GAWTC 3 cut(s) 451, 535, 608
PflFI GACNNNGTC 2 cut(s) 67, 85
PflMI CCANNNNNTGG 1 cut(s) 713
PkrI GCNGC 2 cut(s) 106, 490
PscI ACATGT 1 cut(s) 652
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 2 cut(s) 219, 636
PspPI GGNCC 2 cut(s) 324, 389
PsyI GACNNNGTC 2 cut(s) 67, 85
RseI CAYNNNNRTG 4 cut(s) 309, 341, 562, 651
SalI GTCGAC 1 cut(s) 698
SaqAI TTAA 4 cut(s) 123, 309, 333, 531
SatI GCNGC 2 cut(s) 105, 489
Sau3AI GATC 3 cut(s) 57, 339, 670
Sau96I GGNCC 2 cut(s) 324, 389
ScrFI CCNGG 2 cut(s) 393, 504
SetI ASST 7 cut(s) 71, 201, 248, 309, 490, 520, 640
SfaNI GCATC 5 cut(s) 283, 337, 482, 541, 563
SfuI TTCGAA 1 cut(s) 611
SinI GGWCC 2 cut(s) 324, 389
SmiMI CAYNNNNRTG 4 cut(s) 309, 341, 562, 651
Sse9I AATT 5 cut(s) 5, 168, 229, 297, 618
SsiI CCGC 5 cut(s) 85, 104, 107, 327, 507
SspMI CTAG 5 cut(s) 48, 66, 431, 480, 525
StyD4I CCNGG 2 cut(s) 391, 502
TaaI ACNGT 2 cut(s) 422, 582
TaqI TCGA 8 cut(s) 62, 89, 291, 611, 649, 673, 682, 699
TasI AATT 5 cut(s) 5, 168, 229, 297, 618
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 3 cut(s) 451, 535, 608
Tru1I TTAA 4 cut(s) 123, 309, 333, 531
Tru9I TTAA 4 cut(s) 123, 309, 333, 531
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 3 cut(s) 17, 327, 464
TspGWI ACGGA 1 cut(s) 457
Tth111I GACNNNGTC 2 cut(s) 67, 85
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 2 cut(s) 324, 389
XapI RAATTY 2 cut(s) 5, 229
XbaI TCTAGA 2 cut(s) 479, 524
XceI RCATGY 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 699
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 5 cut(s) 48, 66, 431, 480, 525
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.