Rw6G015560

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000080
Physical Location & Seq
Forward (+)
29934756 .. 29937695
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G015560.1

Sequence Viewer

Length: 2940 bp
ATGACATGTAATCCAAATTGGGAAGAAATAACTGCAGAGTTATTACCTGGTCAAGTGGCACAAGATCGCCCGGATCTAACTACACGAATTTTTCACGCCAAATTTGAAGAGTTAAAGAATGATGTTATTAAAAAAGGAGTCTTGGGTACTGTTGTAGCATACGTATATGTCATCGAGTTTCAAAAACGTGGTCTCCCACACGTTCATATGCTTCTCATGTTAAGTGACGATGATAAACTAAACAACCCAGATGATTATAACCACATTGTTCGGGCTGAAATTCCAGATCAAAATGAAGAGCCAGAGTTATATAATGCGGTGTTGGGGCACATGATACACGGTCCATGTGGGGTACATAGGCAAACTGCTCCATGTATGAAGCGTGGGAGCTGCAAACGAGGCTATCCAAAGCCATTTTCAGCAAACACATTTCAAGGAAATGATTCTTATCCTGTTTATCGTAGACGAGACAATCACTCAGATATGCAATCTAATGGCAATCAAAATAGACCACTGGATAACAGTTGGGTTATCCCCTATAACCCATGGTTACTCACAAAATATGATTGTCATATCAATGTGGAAATCTGTTGCAGCATTAAGAGTGTCAAGTATTTGTATAAATATGTCTATAAAGGTCCAGACAGAGTAACATTTGAAGTTCGACCTGAAGCAAATCAAGATGAAATAAGAAACTTTGTTGATGCAAGATGGGTTTGTGCACCAGAAGCTTTCTGGAGGATGTTTAAATTCGTGATGAATCGAATGTATCCATCAGTGGAACGATTGCAAATTCATCTTCCAGATAGACACAATGTTTTCTTTAATGCCACTGAAAGTGTAACAGAAATTCTGGCAAATGAAAGAAGCTCGATGACGATGCTCACAGAATTTTTTACTAAGAATACTTATTTTGAAGGTGCGCGTCAATACTTATATCGAGAATTTCCTGAGCATTTCAGATGGGATGGGAGACATAAAACATGGGAAGCAAGGGAGACCAACCAAAAAGTCATTGGAAGAATTTACACAGTGTCACCTTCAGAAGGAGAAAAGTTTTACTTGCGTGTTCTTCTTAATCATGTTCGAGGACCTAAGTCATTTGAGGATTTAAGGACATTTGACGGTGTCTTACAACCGACATTCAAGAAAGCAGTTGAACAACGTGGTTTGTTAGAAGATGACGAAAGTATTAGACAATGTCTACTTGAGGCCTCTAATATTCGTATGCCGTCATCTTTAAGAAGATTGTTTGTCACCATCCTGGTTTATTGCATGCCCCATGGTGTGCGCAGTTTGTGGGATGAATTTTATCCATTCATGATAGAGGATTATCCTTCTTCGAGCACCACAGACAATGTACGTATCACAAATAGACTTTTACGAGACTTGAACCAGTTGCTGGTGCAGCACAGTAAGTCTGTCTTTGATTATGACTTGCCAGAAATGACTCAAGATGGTGGTGAAAATTCTTCAATGCCGAGGCTAATACAAGATGAAATTTCAATTAACATTCCTCAAGAAGACTGTGATGCAGTTCATCATTTGAATGAGGATCAAACTTTTGCATACAATTCCATAATATCTGCTATTGAGTGTCATGACAATGCCATATATTTCGTTGATGGTCCAGGAGGAACTGGAAAGACATACTTATATCGTGCATTATTAGCAAGCTTGAGAAGCAATGATCATATTGTATTGGCAACAGCAACTTCTGGCATAGCAGCAACAATACTACCTGGTGGGAGGACAGCACACTCTAGGTTTAAAATTCCTCTTAATCTTGATGCATCTTCGACATGCTCCATTAGTAAACAGTCTGATTTAGCAGAGTTGATCAGAAAGTCATCGGCAATTATTTGGGATGAAGCACCAATGATGAATCGATATGGGTTTGAAGCTCTTGATCGAACATTTAGAGACATAACAAGTGTCGATTTGCCATTTGGAGGAAAGGTGATGATCTTCGGGGGCGATTTCCGACAAGTTCTTCCTGTTATCCATAAGGGTACGAGATCTGAGATGGTGCAAGCCAGTTTGATTAATGCTTCATTTTGGAAGGATGTGAAGATTCTACGTTTGACACAAAATATGAGATCCATTAATGATCCAGAGTTCTCAGAGTTCTTACTTCGTGTTGGTAATGGAGAACAACCAACTGTCGTTGAAGACATAATACAAATACCTTCGCCCATGGTCATATCATGGGAGAATGAAGAATCAATCAACCAGTTGGTTAATCAAGTCTTCCCTAATTTGGATCGTCATGTAAATGATGCCAAGTACATGGTTGAAAGGGCAATAATTACTCCAAAAAATGATGATGTTGACGTACTCAATGAAAGAATCATACAACAATTTCCGGGGCCTGAACGAATCTTATACTCTTTTGACTCAGTTGAGGATGACACCAGAAATATGTATCAACAAGAATTTTTAAATTCAATTTCACCTTCTGGTATGCCGCCACATCAGTTGATTATAAAGAAAGGTGCCCCAATTATGCTTTTGAGAAATATTGACCCAAAGATGGGGTTGTGCAACGGTACAAGATTGACTTGCCGAGGAGCATATAACAATCTAATTGATGCAGAGATTTTAACTGGACAATTTTCGGGAACTAGAGTTTTCTTGCCAAGAATTGCTCTTAAGAGTGCCGAAAGTTCTGGACTCCCTTTTGAGATGACAAGAAAGCAGTTTCCCGTAAAGTTGAGTTTTGCACTTACTATAAATAAATCACAAGGGCAGACAATACCAAATGTTGGTATTTACCTTCCTGATCATGTGTTTAGCCATGGACAGCTGTACGTGGCTTTATCGAGAGGAGTTTCAGAGGCAAGCACCAAAGTTTTGGTAAAAAAAGGTTCATTAGAGGGTGAGGAAGGGGTGTTCACAGCAAACGTTGTGTATAAAGAAATTTTGCTTCCTTCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

979

Amino Acids

111.95

Weight (kDa)

6.05

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 1 - 72 1.2e-26 Helitron helicase-like domain at N-terminus
PIF1 PF05970 516 - 721 2.3e-75 PIF1-like helicase
AAA_30 PF13604 516 - 720 3.1e-06 AAA domain
Pif1_2B_dom PF21530 812 - 854 1.2e-19 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 258, 2486
Acc16I TGCGCA 1 cut(s) 1292
AccB1I GGYRCC 1 cut(s) 2495
AccB7I CCANNNNNTGG 2 cut(s) 1402, 2765
AccI GTMKAC 2 cut(s) 463, 1204
AccII CGCG 1 cut(s) 925
AciI CCGC 2 cut(s) 317, 2468
AclI AACGTT 1 cut(s) 2904
AclWI GGATC 5 cut(s) 81, 1563, 2094, 2105, 2271
AcuI CTGAAG 2 cut(s) 690, 1026
AfaI GTAC 8 cut(s) 148, 354, 1362, 2014, 2288, 2337, 2551, 2810
AfiI CCNNNNNNNGG 7 cut(s) 1046, 1402, 1952, 2260, 2533, 2534, 2765
AflII CTTAAG 1 cut(s) 2651
AflIII ACRYGT 2 cut(s) 5, 199
AjnI CCWGG 4 cut(s) 46, 1263, 1630, 1741
AjuI GAANNNNNNNTTGG 2 cut(s) 400, 432
AloI GAACNNNNNNTCC 2 cut(s) 2876, 2908
AluBI AGCT 6 cut(s) 390, 731, 870, 1677, 1904, 2806
AluI AGCT 6 cut(s) 390, 731, 870, 1677, 1904, 2806
Alw21I GWGCWC 2 cut(s) 724, 1349
Alw26I GTCTC 6 cut(s) 197, 462, 967, 992, 1380, 1917
Alw44I GTGCAC 1 cut(s) 720
AlwI GGATC 5 cut(s) 81, 1563, 2094, 2105, 2271
AlwNI CAGNNNCTG 1 cut(s) 1402
AoxI GGCC 2 cut(s) 1212, 2369
ApaLI GTGCAC 1 cut(s) 720
ApeKI GCWGC 4 cut(s) 390, 594, 1408, 1727
ArsI GACNNNNNNTTYG 2 cut(s) 175, 207
AseI ATTAAT 2 cut(s) 2046, 2106
Asp700I GAANNNNTTC 1 cut(s) 442
AspLEI GCGC 2 cut(s) 925, 1293
AspS9I GGNCC 5 cut(s) 341, 638, 1091, 1628, 2369
AsuC2I CCSGG 2 cut(s) 71, 2367
AsuHPI GGTGA 6 cut(s) 1029, 1249, 1475, 1972, 2445, 2891
AvaII GGWCC 4 cut(s) 341, 638, 1091, 1628
BaeGI GKGCMC 3 cut(s) 330, 724, 2500
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BanI GGYRCC 1 cut(s) 2495
BarI GAAGNNNNNNTAC 2 cut(s) 2163, 2195
BbsI GAAGAC 3 cut(s) 1530, 2178, 2242
Bbv12I GWGCWC 2 cut(s) 724, 1349
BbvI GCAGC 4 cut(s) 377, 606, 1420, 1739
BccI CCATC 9 cut(s) 705, 781, 957, 962, 1268, 1451, 1619, 2020, 2527
BceAI ACGGC 1 cut(s) 1216
BcgI CGANNNNNNTGC 2 cut(s) 862, 896
BciT130I CCWGG 4 cut(s) 48, 1265, 1632, 1743
BciVI GTATCC 1 cut(s) 780
BclI TGATCA 3 cut(s) 1690, 1839, 2782
BcnI CCSGG 2 cut(s) 71, 2367
BcoDI GTCTC 6 cut(s) 197, 462, 967, 992, 1380, 1917
BfaI CTAG 2 cut(s) 1764, 2625
BfmI CTRYAG 1 cut(s) 33
BfrI CTTAAG 1 cut(s) 2651
BfuI GTATCC 1 cut(s) 780
BglII AGATCT 1 cut(s) 2018
BisI GCNGC 5 cut(s) 391, 595, 1409, 1728, 2468
BlsI GCNGC 5 cut(s) 392, 596, 1410, 1729, 2469
Bme1390I CCNGG 6 cut(s) 48, 71, 1265, 1632, 1743, 2367
Bme18I GGWCC 4 cut(s) 341, 638, 1091, 1628
BmgT120I GGNCC 5 cut(s) 341, 638, 1091, 1628, 2369
BmiI GGNNCC 2 cut(s) 2370, 2497
BmrFI CCNGG 6 cut(s) 48, 71, 1265, 1632, 1743, 2367
BmsI GCATC 7 cut(s) 694, 870, 1522, 1780, 1802, 2269, 2581
BoxI GACNNNNGTC 1 cut(s) 1096
BpiI GAAGAC 3 cut(s) 1530, 2178, 2242
BpmI CTGGAG 1 cut(s) 757
Bpu10I CCTNAGC 1 cut(s) 951
BpuEI CTTGAG 4 cut(s) 1229, 1437, 1503, 1699
BpuMI CCSGG 2 cut(s) 71, 2367
Bsa29I ATCGAT 1 cut(s) 1888
BsaAI YACGTR 3 cut(s) 163, 1364, 2812
BsaBI GATNNNNATC 1 cut(s) 447
BsaI GGTCTC 2 cut(s) 197, 992
BsaJI CCNNGG 7 cut(s) 545, 1282, 1481, 2196, 2366, 2566, 2797
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 1944, 1974
Bsc4I CCNNNNNNNGG 7 cut(s) 1046, 1402, 1952, 2260, 2533, 2534, 2765
Bse1I ACTGG 6 cut(s) 519, 1396, 1645, 2037, 2233, 2611
Bse3DI GCAATG 1 cut(s) 1693
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 4 cut(s) 48, 1265, 1632, 1743
BseCI ATCGAT 1 cut(s) 1888
BseDI CCNNGG 7 cut(s) 545, 1282, 1481, 2196, 2366, 2566, 2797
BseGI GGATG 7 cut(s) 747, 973, 1260, 1309, 1873, 2071, 2413
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 7 cut(s) 1046, 1402, 1952, 2260, 2533, 2534, 2765
BseMI GCAATG 1 cut(s) 1693
BseMII CTCAG 5 cut(s) 492, 942, 2013, 2136, 2412
BseNI ACTGG 6 cut(s) 519, 1396, 1645, 2037, 2233, 2611
BseRI GAGGAG 2 cut(s) 2583, 2841
BseSI GKGCMC 3 cut(s) 330, 724, 2500
BseXI GCAGC 4 cut(s) 377, 606, 1420, 1739
BsgI GTGCAG 1 cut(s) 1427
Bsh1236I CGCG 1 cut(s) 925
BshFI GGCC 2 cut(s) 1214, 2371
BshNI GGYRCC 1 cut(s) 2495
BshVI ATCGAT 1 cut(s) 1888
BsiHKAI GWGCWC 2 cut(s) 724, 1349
BsiSI CCGG 2 cut(s) 71, 2366
BslI CCNNNNNNNGG 7 cut(s) 1046, 1402, 1952, 2260, 2533, 2534, 2765
BsmAI GTCTC 6 cut(s) 197, 462, 967, 992, 1380, 1917
BsnI GGCC 2 cut(s) 1214, 2371
Bso31I GGTCTC 2 cut(s) 197, 992
Bsp1286I GDGCHC 4 cut(s) 330, 724, 1349, 2500
Bsp19I CCATGG 4 cut(s) 545, 1282, 2196, 2797
BspACI CCGC 2 cut(s) 317, 2468
BspANI GGCC 2 cut(s) 1214, 2371
BspCNI CTCAG 5 cut(s) 491, 943, 2014, 2135, 2411
BspDI ATCGAT 1 cut(s) 1888
BspFNI CGCG 1 cut(s) 925
BspHI TCATGA 2 cut(s) 1320, 1600
BspLI GGNNCC 2 cut(s) 2370, 2497
BspMAI CTGCAG 1 cut(s) 37
BspPI GGATC 5 cut(s) 81, 1563, 2094, 2105, 2271
BspQI GCTCTTC 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 2495
BspTI CTTAAG 1 cut(s) 2651
BspTNI GGTCTC 2 cut(s) 197, 992
BsrDI GCAATG 1 cut(s) 1693
BsrI ACTGG 6 cut(s) 519, 1396, 1645, 2037, 2233, 2611
BssECI CCNNGG 7 cut(s) 545, 1282, 1481, 2196, 2366, 2566, 2797
BssT1I CCWWGG 4 cut(s) 545, 1282, 2196, 2797
Bst2UI CCWGG 4 cut(s) 48, 1265, 1632, 1743
Bst6I CTCTTC 2 cut(s) 102, 291
BstAFI CTTAAG 1 cut(s) 2651
BstBAI YACGTR 3 cut(s) 163, 1364, 2812
BstC8I GCNNGC 4 cut(s) 1277, 1675, 2034, 2842
BstDEI CTNAG 7 cut(s) 478, 900, 951, 1095, 2022, 2122, 2398
BstDSI CCRYGG 4 cut(s) 545, 1282, 2196, 2797
BstENI CCTNNNNNAGG 1 cut(s) 1044
BstF5I GGATG 7 cut(s) 747, 973, 1260, 1309, 1873, 2071, 2413
BstFNI CGCG 1 cut(s) 925
BstHHI GCGC 2 cut(s) 925, 1293
BstMAI GTCTC 6 cut(s) 197, 462, 967, 992, 1380, 1917
BstMWI GCNNNNNNNGC 5 cut(s) 399, 728, 1408, 1670, 1683
BstNI CCWGG 4 cut(s) 48, 1265, 1632, 1743
BstNSI RCATGY 3 cut(s) 9, 1279, 1806
BstPAI GACNNNNGTC 1 cut(s) 1096
BstSCI CCNGG 6 cut(s) 46, 69, 1263, 1630, 1741, 2365
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 3 cut(s) 330, 724, 2500
BstSNI TACGTA 2 cut(s) 163, 1364
BstUI CGCG 1 cut(s) 925
BstV1I GCAGC 4 cut(s) 377, 606, 1420, 1739
BstV2I GAAGAC 3 cut(s) 1530, 2178, 2242
BstX2I RGATCY 3 cut(s) 73, 2018, 2099
BstXI CCANNNNNNTGG 2 cut(s) 2290, 2854
BstYI RGATCY 3 cut(s) 73, 2018, 2099
Bsu15I ATCGAT 1 cut(s) 1888
BsuI GTATCC 1 cut(s) 780
BsuRI GGCC 2 cut(s) 1214, 2371
BsuTUI ATCGAT 1 cut(s) 1888
BtgI CCRYGG 4 cut(s) 545, 1282, 2196, 2797
BtsCI GGATG 7 cut(s) 747, 973, 1260, 1309, 1873, 2071, 2413
BtsIMutI CAGTG 4 cut(s) 512, 783, 831, 1038
Cac8I GCNNGC 4 cut(s) 1277, 1675, 2034, 2842
CaiI CAGNNNCTG 1 cut(s) 1402
CciI TCATGA 2 cut(s) 1320, 1600
CfoI GCGC 2 cut(s) 925, 1293
Cfr13I GGNCC 5 cut(s) 341, 638, 1091, 1628, 2369
ClaI ATCGAT 1 cut(s) 1888
CseI GACGC 1 cut(s) 914
CsiI ACCWGGT 2 cut(s) 46, 1741
Csp6I GTAC 8 cut(s) 147, 353, 1361, 2013, 2287, 2336, 2550, 2809
CspCI CAANNNNNGTGG 2 cut(s) 2460, 2495
CviQI GTAC 8 cut(s) 147, 353, 1361, 2013, 2287, 2336, 2550, 2809
DdeI CTNAG 7 cut(s) 478, 900, 951, 1095, 2022, 2122, 2398
DraI TTTAAA 3 cut(s) 748, 1771, 2442
Eam1104I CTCTTC 2 cut(s) 102, 291
EarI CTCTTC 2 cut(s) 102, 291
Eco105I TACGTA 2 cut(s) 163, 1364
Eco130I CCWWGG 4 cut(s) 545, 1282, 2196, 2797
Eco147I AGGCCT 1 cut(s) 1214
Eco31I GGTCTC 2 cut(s) 197, 992
Eco47I GGWCC 4 cut(s) 341, 638, 1091, 1628
Eco57I CTGAAG 2 cut(s) 690, 1026
EcoNI CCTNNNNNAGG 1 cut(s) 1044
EcoO109I RGGNCCY 2 cut(s) 1091, 2369
EcoRII CCWGG 4 cut(s) 46, 1263, 1630, 1741
EcoT14I CCWWGG 4 cut(s) 545, 1282, 2196, 2797
EcoT22I ATGCAT 1 cut(s) 1795
ErhI CCWWGG 4 cut(s) 545, 1282, 2196, 2797
FalI AAGNNNNNCTT 8 cut(s) 1046, 1078, 1409, 1441, 1637, 1669, 2545, 2577
FauNDI CATATG 1 cut(s) 207
FbaI TGATCA 3 cut(s) 1690, 1839, 2782
FblI GTMKAC 2 cut(s) 463, 1204
Fnu4HI GCNGC 5 cut(s) 391, 595, 1409, 1728, 2468
FokI GGATG 7 cut(s) 754, 980, 1247, 1316, 1880, 2078, 2420
Fsp4HI GCNGC 5 cut(s) 391, 595, 1409, 1728, 2468
FspBI CTAG 2 cut(s) 1764, 2625
FspI TGCGCA 1 cut(s) 1292
GlaI GCGC 2 cut(s) 924, 1292
GluI GCNGC 5 cut(s) 391, 595, 1409, 1728, 2468
GsuI CTGGAG 1 cut(s) 757
HaeIII GGCC 2 cut(s) 1214, 2371
HapII CCGG 2 cut(s) 71, 2366
HgaI GACGC 1 cut(s) 914
HhaI GCGC 2 cut(s) 925, 1293
Hin6I GCGC 2 cut(s) 923, 1291
HinP1I GCGC 2 cut(s) 923, 1291
HincII GTYRAC 1 cut(s) 2332
HindII GTYRAC 1 cut(s) 2332
HindIII AAGCTT 2 cut(s) 729, 1675
HpaII CCGG 2 cut(s) 71, 2366
HphI GGTGA 6 cut(s) 1029, 1249, 1475, 1972, 2445, 2891
Hpy166II GTNNAC 6 cut(s) 464, 722, 1205, 1817, 2332, 2895
Hpy188I TCNGA 9 cut(s) 481, 962, 1045, 1825, 1844, 1985, 2023, 2125, 2836
Hpy8I GTNNAC 6 cut(s) 464, 722, 1205, 1817, 2332, 2895
HpyCH4IV ACGT 9 cut(s) 162, 187, 201, 1165, 1363, 2080, 2334, 2811, 2904
HpyF10VI GCNNNNNNNGC 5 cut(s) 399, 728, 1408, 1670, 1683
HpyF3I CTNAG 7 cut(s) 478, 900, 951, 1095, 2022, 2122, 2398
HpySE526I ACGT 9 cut(s) 162, 187, 201, 1165, 1363, 2080, 2334, 2811, 2904
HspAI GCGC 2 cut(s) 923, 1291
Ksp22I TGATCA 3 cut(s) 1690, 1839, 2782
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 4 cut(s) 373, 387, 1811, 2570
Lsp1109I GCAGC 4 cut(s) 377, 606, 1420, 1739
LweI GCATC 7 cut(s) 694, 870, 1522, 1780, 1802, 2269, 2581
MabI ACCWGGT 2 cut(s) 46, 1741
MaeI CTAG 2 cut(s) 1764, 2625
MaeII ACGT 9 cut(s) 162, 187, 201, 1165, 1363, 2080, 2334, 2811, 2904
MaeIII GTNAC 6 cut(s) 224, 549, 649, 841, 1035, 1255
MflI RGATCY 3 cut(s) 73, 2018, 2099
MhlI GDGCHC 4 cut(s) 330, 724, 1349, 2500
MlyI GAGTC 4 cut(s) 147, 1444, 2390, 2667
MmeI TCCRAC 1 cut(s) 2008
Mph1103I ATGCAT 1 cut(s) 1795
MroXI GAANNNNTTC 1 cut(s) 442
MslI CAYNNNNRTG 4 cut(s) 576, 1548, 1605, 2274
MspA1I CMGCKG 1 cut(s) 2806
MspCI CTTAAG 1 cut(s) 2651
MspI CCGG 2 cut(s) 71, 2366
MspR9I CCNGG 6 cut(s) 48, 71, 1265, 1632, 1743, 2367
MvaI CCWGG 4 cut(s) 48, 1265, 1632, 1743
MvnI CGCG 1 cut(s) 925
MwoI GCNNNNNNNGC 5 cut(s) 399, 728, 1408, 1670, 1683
NciI CCSGG 2 cut(s) 71, 2367
NcoI CCATGG 4 cut(s) 545, 1282, 2196, 2797
NdeI CATATG 1 cut(s) 207
NlaIV GGNNCC 2 cut(s) 2370, 2497
NmeAIII GCCGAG 2 cut(s) 1506, 2591
NmuCI GTSAC 3 cut(s) 224, 1035, 1255
NsbI TGCGCA 1 cut(s) 1292
NsiI ATGCAT 1 cut(s) 1795
NspI RCATGY 3 cut(s) 9, 1279, 1806
PaeI GCATGC 1 cut(s) 1279
PagI TCATGA 2 cut(s) 1320, 1600
PceI AGGCCT 1 cut(s) 1214
PciI ACATGT 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 442
PfeI GAWTC 7 cut(s) 443, 760, 1885, 2074, 2222, 2349, 2379
PflFI GACNNNGTC 2 cut(s) 1127, 1200
PflMI CCANNNNNTGG 2 cut(s) 1402, 2765
PfoI TCCNGGA 1 cut(s) 1630
PkrI GCNGC 5 cut(s) 392, 596, 1410, 1729, 2469
PleI GAGTC 4 cut(s) 146, 1444, 2390, 2667
PpsI GAGTC 4 cut(s) 146, 1444, 2390, 2667
Ppu21I YACGTR 3 cut(s) 163, 1364, 2812
PpuMI RGGWCCY 1 cut(s) 1091
PscI ACATGT 1 cut(s) 5
PshAI GACNNNNGTC 1 cut(s) 1096
PshBI ATTAAT 2 cut(s) 2046, 2106
PsiI TTATAA 2 cut(s) 258, 2486
Psp1406I AACGTT 1 cut(s) 2904
Psp5II RGGWCCY 1 cut(s) 1091
Psp6I CCWGG 4 cut(s) 46, 1263, 1630, 1741
PspGI CCWGG 4 cut(s) 46, 1263, 1630, 1741
PspN4I GGNNCC 2 cut(s) 2370, 2497
PspPI GGNCC 5 cut(s) 341, 638, 1091, 1628, 2369
PspPPI RGGWCCY 1 cut(s) 1091
PstI CTGCAG 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 1402
PsuI RGATCY 3 cut(s) 73, 2018, 2099
PsyI GACNNNGTC 2 cut(s) 1127, 1200
PvuII CAGCTG 1 cut(s) 2806
RsaI GTAC 8 cut(s) 148, 354, 1362, 2014, 2288, 2337, 2551, 2810
RsaNI GTAC 8 cut(s) 147, 353, 1361, 2013, 2287, 2336, 2550, 2809
RseI CAYNNNNRTG 4 cut(s) 576, 1548, 1605, 2274
SapI GCTCTTC 1 cut(s) 291
SatI GCNGC 5 cut(s) 391, 595, 1409, 1728, 2468
Sau96I GGNCC 5 cut(s) 341, 638, 1091, 1628, 2369
SchI GAGTC 4 cut(s) 147, 1444, 2390, 2667
ScrFI CCNGG 6 cut(s) 48, 71, 1265, 1632, 1743, 2367
SduI GDGCHC 4 cut(s) 330, 724, 1349, 2500
SexAI ACCWGGT 2 cut(s) 46, 1741
SfaNI GCATC 7 cut(s) 694, 870, 1522, 1780, 1802, 2269, 2581
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 4 cut(s) 341, 638, 1091, 1628
SmiMI CAYNNNNRTG 4 cut(s) 576, 1548, 1605, 2274
SmlI CTYRAG 5 cut(s) 1208, 1452, 1518, 1678, 2651
SmoI CTYRAG 5 cut(s) 1208, 1452, 1518, 1678, 2651
SnaBI TACGTA 2 cut(s) 163, 1364
SphI GCATGC 1 cut(s) 1279
SseBI AGGCCT 1 cut(s) 1214
SsiI CCGC 2 cut(s) 317, 2468
SspI AATATT 2 cut(s) 1222, 2521
SspMI CTAG 2 cut(s) 1764, 2625
StuI AGGCCT 1 cut(s) 1214
StyD4I CCNGG 6 cut(s) 46, 69, 1263, 1630, 1741, 2365
StyI CCWWGG 4 cut(s) 545, 1282, 2196, 2797
TaiI ACGT 9 cut(s) 165, 190, 204, 1168, 1366, 2083, 2337, 2814, 2907
TatI WGTACW 1 cut(s) 2286
TauI GCSGC 1 cut(s) 2470
TfiI GAWTC 7 cut(s) 443, 760, 1885, 2074, 2222, 2349, 2379
TscAI CASTG 4 cut(s) 519, 783, 838, 1038
TseFI GTSAC 3 cut(s) 224, 1035, 1255
TseI GCWGC 4 cut(s) 390, 594, 1408, 1727
Tsp45I GTSAC 3 cut(s) 224, 1035, 1255
TspRI CASTG 4 cut(s) 519, 783, 838, 1038
Tth111I GACNNNGTC 2 cut(s) 1127, 1200
Van91I CCANNNNNTGG 2 cut(s) 1402, 2765
Vha464I CTTAAG 1 cut(s) 2651
VneI GTGCAC 1 cut(s) 720
VpaK11BI GGWCC 4 cut(s) 341, 638, 1091, 1628
VspI ATTAAT 2 cut(s) 2046, 2106
XagI CCTNNNNNAGG 1 cut(s) 1044
XceI RCATGY 3 cut(s) 9, 1279, 1806
XcmI CCANNNNNNNNNTGG 2 cut(s) 732, 1013
XmiI GTMKAC 2 cut(s) 463, 1204
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 2 cut(s) 1764, 2625
Zsp2I ATGCAT 1 cut(s) 1795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.