Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 15 |
| AciI | CCGC | 3 cut(s) | 23, 386, 412 |
| AcsI | RAATTY | 2 cut(s) | 176, 299 |
| AcuI | CTGAAG | 1 cut(s) | 195 |
| AgsI | TTSAA | 1 cut(s) | 362 |
| AluBI | AGCT | 2 cut(s) | 107, 328 |
| AluI | AGCT | 2 cut(s) | 107, 328 |
| Alw21I | GWGCWC | 1 cut(s) | 293 |
| AoxI | GGCC | 1 cut(s) | 81 |
| ApoI | RAATTY | 2 cut(s) | 176, 299 |
| AsuHPI | GGTGA | 1 cut(s) | 227 |
| AsuII | TTCGAA | 1 cut(s) | 160 |
| Bbv12I | GWGCWC | 1 cut(s) | 293 |
| BccI | CCATC | 1 cut(s) | 109 |
| BfaI | CTAG | 2 cut(s) | 294, 329 |
| BglII | AGATCT | 1 cut(s) | 256 |
| BmsI | GCATC | 2 cut(s) | 69, 391 |
| Bpu14I | TTCGAA | 1 cut(s) | 160 |
| Bse1I | ACTGG | 1 cut(s) | 157 |
| BseGI | GGATG | 1 cut(s) | 60 |
| BseNI | ACTGG | 1 cut(s) | 157 |
| BshFI | GGCC | 1 cut(s) | 83 |
| BsiHKAI | GWGCWC | 1 cut(s) | 293 |
| BslFI | GGGAC | 1 cut(s) | 185 |
| BsmFI | GGGAC | 1 cut(s) | 185 |
| BsmI | GAATGC | 1 cut(s) | 351 |
| BsnI | GGCC | 1 cut(s) | 83 |
| Bsp119I | TTCGAA | 1 cut(s) | 160 |
| Bsp1286I | GDGCHC | 1 cut(s) | 293 |
| Bsp143I | GATC | 4 cut(s) | 192, 217, 256, 304 |
| BspACI | CCGC | 3 cut(s) | 23, 386, 412 |
| BspANI | GGCC | 1 cut(s) | 83 |
| BspT104I | TTCGAA | 1 cut(s) | 160 |
| BsrI | ACTGG | 1 cut(s) | 157 |
| BssMI | GATC | 4 cut(s) | 192, 217, 256, 304 |
| Bst6I | CTCTTC | 1 cut(s) | 211 |
| BstBI | TTCGAA | 1 cut(s) | 160 |
| BstC8I | GCNNGC | 1 cut(s) | 98 |
| BstF5I | GGATG | 1 cut(s) | 60 |
| BstKTI | GATC | 4 cut(s) | 195, 220, 259, 307 |
| BstMBI | GATC | 4 cut(s) | 192, 217, 256, 304 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 316, 325 |
| BstNSI | RCATGY | 1 cut(s) | 100 |
| BstX2I | RGATCY | 1 cut(s) | 256 |
| BstYI | RGATCY | 1 cut(s) | 256 |
| BsuRI | GGCC | 1 cut(s) | 83 |
| BtsCI | GGATG | 1 cut(s) | 60 |
| Cac8I | GCNNGC | 1 cut(s) | 98 |
| CviAII | CATG | 2 cut(s) | 97, 190 |
| CviJI | RGCY | 4 cut(s) | 83, 107, 247, 328 |
| CviKI_1 | RGCY | 4 cut(s) | 83, 107, 247, 328 |
| DpnI | GATC | 4 cut(s) | 194, 219, 258, 306 |
| DpnII | GATC | 4 cut(s) | 192, 217, 256, 304 |
| Eam1104I | CTCTTC | 1 cut(s) | 211 |
| EarI | CTCTTC | 1 cut(s) | 211 |
| Eco147I | AGGCCT | 1 cut(s) | 83 |
| Eco57I | CTGAAG | 1 cut(s) | 195 |
| FaeI | CATG | 2 cut(s) | 100, 193 |
| FaiI | YATR | 5 cut(s) | 15, 98, 141, 191, 377 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 354, 386 |
| FaqI | GGGAC | 1 cut(s) | 185 |
| FatI | CATG | 2 cut(s) | 96, 189 |
| FauI | CCCGC | 1 cut(s) | 393 |
| FokI | GGATG | 1 cut(s) | 47 |
| FspBI | CTAG | 2 cut(s) | 294, 329 |
| HaeIII | GGCC | 1 cut(s) | 83 |
| Hin1II | CATG | 2 cut(s) | 100, 193 |
| HincII | GTYRAC | 1 cut(s) | 146 |
| HindII | GTYRAC | 1 cut(s) | 146 |
| HphI | GGTGA | 1 cut(s) | 227 |
| Hpy166II | GTNNAC | 1 cut(s) | 146 |
| Hpy188I | TCNGA | 2 cut(s) | 65, 239 |
| Hpy188III | TCNNGA | 1 cut(s) | 254 |
| Hpy8I | GTNNAC | 1 cut(s) | 146 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 404 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 316, 325 |
| Hsp92II | CATG | 2 cut(s) | 100, 193 |
| Kzo9I | GATC | 4 cut(s) | 192, 217, 256, 304 |
| LpnPI | CCDG | 3 cut(s) | 138, 198, 229 |
| LweI | GCATC | 2 cut(s) | 69, 391 |
| MaeI | CTAG | 2 cut(s) | 294, 329 |
| MaeIII | GTNAC | 1 cut(s) | 124 |
| MalI | GATC | 4 cut(s) | 194, 219, 258, 306 |
| MboI | GATC | 4 cut(s) | 192, 217, 256, 304 |
| MboII | GAAGA | 9 cut(s) | 59, 126, 174, 198, 201, 209, 344, 374, 404 |
| MfeI | CAATTG | 1 cut(s) | 405 |
| MflI | RGATCY | 1 cut(s) | 256 |
| MhlI | GDGCHC | 1 cut(s) | 293 |
| MluCI | AATT | 6 cut(s) | 90, 129, 176, 278, 299, 405 |
| MmeI | TCCRAC | 3 cut(s) | 24, 88, 252 |
| MnlI | CCTC | 3 cut(s) | 29, 73, 94 |
| MseI | TTAA | 2 cut(s) | 282, 417 |
| MunI | CAATTG | 1 cut(s) | 405 |
| Mva1269I | GAATGC | 1 cut(s) | 351 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 316, 325 |
| NdeII | GATC | 4 cut(s) | 192, 217, 256, 304 |
| NlaIII | CATG | 2 cut(s) | 100, 193 |
| NmeAIII | GCCGAG | 1 cut(s) | 375 |
| NmuCI | GTSAC | 1 cut(s) | 124 |
| NspI | RCATGY | 1 cut(s) | 100 |
| NspV | TTCGAA | 1 cut(s) | 160 |
| PaeI | GCATGC | 1 cut(s) | 100 |
| PceI | AGGCCT | 1 cut(s) | 83 |
| PctI | GAATGC | 1 cut(s) | 351 |
| PsiI | TTATAA | 1 cut(s) | 15 |
| PsuI | RGATCY | 1 cut(s) | 256 |
| SaqAI | TTAA | 2 cut(s) | 282, 417 |
| Sau3AI | GATC | 4 cut(s) | 192, 217, 256, 304 |
| SduI | GDGCHC | 1 cut(s) | 293 |
| SetI | ASST | 4 cut(s) | 40, 109, 217, 330 |
| SfaNI | GCATC | 2 cut(s) | 69, 391 |
| SfuI | TTCGAA | 1 cut(s) | 160 |
| SphI | GCATGC | 1 cut(s) | 100 |
| Sse9I | AATT | 6 cut(s) | 90, 129, 176, 278, 299, 405 |
| SseBI | AGGCCT | 1 cut(s) | 83 |
| SsiI | CCGC | 3 cut(s) | 23, 386, 412 |
| SspI | AATATT | 1 cut(s) | 229 |
| SspMI | CTAG | 2 cut(s) | 294, 329 |
| StuI | AGGCCT | 1 cut(s) | 83 |
| TaqI | TCGA | 3 cut(s) | 160, 195, 253 |
| TasI | AATT | 6 cut(s) | 90, 129, 176, 278, 299, 405 |
| Tru1I | TTAA | 2 cut(s) | 282, 417 |
| Tru9I | TTAA | 2 cut(s) | 282, 417 |
| TseFI | GTSAC | 1 cut(s) | 124 |
| Tsp45I | GTSAC | 1 cut(s) | 124 |
| TspDTI | ATGAA | 1 cut(s) | 381 |
| XapI | RAATTY | 2 cut(s) | 176, 299 |
| XceI | RCATGY | 1 cut(s) | 100 |
| XspI | CTAG | 2 cut(s) | 294, 329 |