Rw1G010010

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000080
Physical Location & Seq
Forward (+)
22243057 .. 22244331
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010010.1

Sequence Viewer

Length: 1275 bp
ATGAATCAAATAGTCTTAGCCACAGCATCATCTGGAATAGCTACAAATATACTGCCTAGCGGGAGGACAGCTCACTCTAGATTTAAGATTCTACTCAATGTAGATGCGTCATCTATGTGTTCCATAAGTAAACAGTCTGCTCTGGCACAACTGATACAAGACTCGACTGCAATTATTTGGGATGAGGCACCTATGACACATCGACATGTCTTTGAAGCACTTGATCGAACTTTTCGAGACATAATGGATGTCGACTTACCATTTGGTGGAAAGATAATGATTTTTGGGGGAGATTTTCGTCAAGTCCTCCCTGTTATTCCTAAAGGAACGAAATCTGAACTCATTCAAGCCTCCATTGTGAAATCAACATTTTGGGCACAGATAAAGATATTGACACTGAGACAGAATATGAGATCCATAAATGACCACCAATTTGCAGAGTTTCTTCTTCGTGTTGGCGATGGGAGTGAACAAGTCATCAATGACGAAATGATAAGATTACCGGAATGCATGGTGGTTCCATGGGAAAGCGACCAATCTATCAATCTAATTATTGATGAAGTTTTCCCCAATTTGGGTGATCATGTAAATGATGCAAGATACATGGTGGACAGAGCATTGATTACTCCTATAAACGACGATGTGGACGTGTTGAATGAAAAGATAATCAATATGTTTCCAGGTGAAGAAATTACATTGTACTCATTTGACTCTGTTGAAGATGACATCAGAAATTTGTACCAACCAGAGTTTCTAAACTCAATAACCGCAGGTGGGCTGCCACCCCACAAGTTAACTTTGAAAGTAGGTGCTCCAATCATGCTTTTAAGGAATATTGACCCTAAATTGGGATTGTGTAACGGTACTAGATTGTTGTGCCGTGGTTCGTACCAAAATCTGATTGATGCTGAGATACTAACAGGACAATTTGCTAGGACTAGAGTTTTCCTACCGAGAATTCCTTTGAAAAGTGCAGAAAATGCTGGGCTACTATTTCAACTAACAAGAAAGCAATTTCCAGTCAAACTGAGCTTCTGTTTGACCATAAACAAATCTCAAGGCCAGACTATCCCACATGTAGGTGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTATATGTGGCATTGTCGAGAGGAGTATCGGAGTCAACCACAAAAGTTTTGGTTAAAAAAGGATCCATAGTTGGTGAAGATGGTGTATTTACGAAGAATGTCGTATTTAAAGAAGTTTTGCTTCATTCAAACACAAATGAAGGATCATCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

424

Amino Acids

47.15

Weight (kDa)

6.08

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 2 - 159 1.5e-56 PIF1-like helicase
Pif1_2B_dom PF21530 250 - 292 2.4e-21 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 761
AccB1I GGYRCC 1 cut(s) 187
AccB7I CCANNNNNTGG 1 cut(s) 266
AccI GTMKAC 1 cut(s) 252
AciI CCGC 2 cut(s) 60, 768
AclWI GGATC 4 cut(s) 408, 1176, 1189, 1270
AcsI RAATTY 2 cut(s) 733, 957
AfaI GTAC 4 cut(s) 701, 740, 865, 890
AfiI CCNNNNNNNGG 7 cut(s) 266, 574, 575, 774, 847, 848, 1079
AflIII ACRYGT 3 cut(s) 205, 648, 1075
AgsI TTSAA 8 cut(s) 215, 347, 655, 719, 802, 967, 998, 1248
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 1 cut(s) 679
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
AluBI AGCT 3 cut(s) 41, 71, 1032
AluI AGCT 3 cut(s) 41, 71, 1032
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 2 cut(s) 231, 394
AlwI GGATC 4 cut(s) 408, 1176, 1189, 1270
AoxI GGCC 1 cut(s) 1060
ApeKI GCWGC 1 cut(s) 778
ApoI RAATTY 2 cut(s) 733, 957
ArsI GACNNNNNNTTYG 4 cut(s) 245, 277, 416, 448
Asp700I GAANNNNTTC 1 cut(s) 342
AsuHPI GGTGA 3 cut(s) 590, 695, 1205
BaeGI GKGCMC 1 cut(s) 379
BamHI GGATCC 1 cut(s) 1181
BanI GGYRCC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 1 cut(s) 765
BccI CCATC 2 cut(s) 455, 1193
BceAI ACGGC 1 cut(s) 864
BciT130I CCWGG 1 cut(s) 681
BclI TGATCA 1 cut(s) 580
BcoDI GTCTC 2 cut(s) 231, 394
BfaI CTAG 5 cut(s) 57, 78, 867, 933, 939
BfuAI ACCTGC 1 cut(s) 761
BisI GCNGC 1 cut(s) 779
BlsI GCNGC 1 cut(s) 780
Bme1390I CCNGG 1 cut(s) 681
BmgBI CACGTC 1 cut(s) 649
BmiI GGNNCC 3 cut(s) 189, 519, 1183
BmrFI CCNGG 1 cut(s) 681
BmsI GCATC 4 cut(s) 35, 94, 583, 895
BplI GAGNNNNNCTC 2 cut(s) 55, 87
BpuEI CTTGAG 1 cut(s) 1041
BsaJI CCNNGG 2 cut(s) 521, 880
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 574, 575, 774, 847, 848, 1079
Bse1I ACTGG 1 cut(s) 1019
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 2 cut(s) 521, 880
BseGI GGATG 2 cut(s) 187, 253
BseLI CCNNNNNNNGG 7 cut(s) 266, 574, 575, 774, 847, 848, 1079
BseMII CTCAG 3 cut(s) 389, 900, 1019
BseNI ACTGG 1 cut(s) 1019
BseRI GAGGAG 1 cut(s) 1155
BseSI GKGCMC 1 cut(s) 379
BseXI GCAGC 1 cut(s) 765
BseYI CCCAGC 1 cut(s) 983
BsgI GTGCAG 1 cut(s) 993
BshFI GGCC 1 cut(s) 1062
BshNI GGYRCC 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 1 cut(s) 503
BslI CCNNNNNNNGG 7 cut(s) 266, 574, 575, 774, 847, 848, 1079
BsmAI GTCTC 2 cut(s) 231, 394
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 1 cut(s) 1062
Bsp1286I GDGCHC 2 cut(s) 379, 814
Bsp143I GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
Bsp19I CCATGG 1 cut(s) 521
BspACI CCGC 2 cut(s) 60, 768
BspANI GGCC 1 cut(s) 1062
BspCNI CTCAG 3 cut(s) 390, 901, 1020
BspLI GGNNCC 3 cut(s) 189, 519, 1183
BspMI ACCTGC 1 cut(s) 761
BspPI GGATC 4 cut(s) 408, 1176, 1189, 1270
BspT107I GGYRCC 1 cut(s) 187
BsrI ACTGG 1 cut(s) 1019
BssECI CCNNGG 2 cut(s) 521, 880
BssMI GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
BssT1I CCWWGG 1 cut(s) 521
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 2 cut(s) 135, 863
BstAPI GCANNNNNTGC 1 cut(s) 980
BstDEI CTNAG 4 cut(s) 16, 398, 909, 1028
BstDSI CCRYGG 2 cut(s) 521, 880
BstF5I GGATG 2 cut(s) 187, 253
BstKTI GATC 6 cut(s) 226, 416, 583, 1099, 1184, 1265
BstMAI GTCTC 2 cut(s) 231, 394
BstMBI GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
BstMWI GCNNNNNNNGC 1 cut(s) 980
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 2 cut(s) 209, 1079
BstSCI CCNGG 1 cut(s) 679
BstSLI GKGCMC 1 cut(s) 379
BstV1I GCAGC 1 cut(s) 765
BstX2I RGATCY 2 cut(s) 413, 1181
BstYI RGATCY 2 cut(s) 413, 1181
BsuRI GGCC 1 cut(s) 1062
BtgI CCRYGG 2 cut(s) 521, 880
BtgZI GCGATG 1 cut(s) 474
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 2 cut(s) 187, 253
BtsIMutI CAGTG 1 cut(s) 395
BveI ACCTGC 1 cut(s) 761
CseI GACGC 1 cut(s) 96
Csp6I GTAC 4 cut(s) 700, 739, 864, 889
CviAII CATG 9 cut(s) 206, 511, 522, 584, 604, 820, 1076, 1100, 1112
CviJI RGCY 8 cut(s) 20, 41, 71, 350, 778, 988, 1032, 1062
CviKI_1 RGCY 8 cut(s) 20, 41, 71, 350, 778, 988, 1032, 1062
CviQI GTAC 4 cut(s) 700, 739, 864, 889
DdeI CTNAG 4 cut(s) 16, 398, 909, 1028
DpnI GATC 6 cut(s) 225, 415, 582, 1098, 1183, 1264
DpnII GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
DraI TTTAAA 1 cut(s) 1228
Eco130I CCWWGG 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 957
EcoRII CCWGG 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 521
EcoT22I ATGCAT 1 cut(s) 512
ErhI CCWWGG 1 cut(s) 521
FaeI CATG 9 cut(s) 209, 514, 525, 587, 607, 823, 1079, 1103, 1115
FalI AAGNNNNNCTT 2 cut(s) 1224, 1256
FatI CATG 9 cut(s) 205, 510, 521, 583, 603, 819, 1075, 1099, 1111
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 1 cut(s) 779
FokI GGATG 2 cut(s) 194, 260
Fsp4HI GCNGC 1 cut(s) 779
FspBI CTAG 5 cut(s) 57, 78, 867, 933, 939
GluI GCNGC 1 cut(s) 779
GsaI CCCAGC 1 cut(s) 987
HaeIII GGCC 1 cut(s) 1062
HapII CCGG 1 cut(s) 503
HgaI GACGC 1 cut(s) 96
Hin1II CATG 9 cut(s) 209, 514, 525, 587, 607, 823, 1079, 1103, 1115
HincII GTYRAC 3 cut(s) 253, 795, 1155
HindII GTYRAC 3 cut(s) 253, 795, 1155
HinfI GANTC 5 cut(s) 4, 88, 161, 710, 1151
HpaI GTTAAC 1 cut(s) 795
HpaII CCGG 1 cut(s) 503
HphI GGTGA 3 cut(s) 590, 695, 1205
Hpy166II GTNNAC 8 cut(s) 131, 253, 470, 610, 646, 795, 1087, 1155
Hpy188I TCNGA 4 cut(s) 337, 731, 900, 1150
Hpy188III TCNNGA 4 cut(s) 33, 78, 236, 1137
Hpy8I GTNNAC 8 cut(s) 131, 253, 470, 610, 646, 795, 1087, 1155
Hpy99I CGWCG 1 cut(s) 641
HpyAV CCTTC 1 cut(s) 1253
HpyCH4III ACNGT 2 cut(s) 135, 863
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 5 cut(s) 170, 437, 510, 596, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 980
HpyF3I CTNAG 4 cut(s) 16, 398, 909, 1028
HpySE526I ACGT 1 cut(s) 648
Hsp92II CATG 9 cut(s) 209, 514, 525, 587, 607, 823, 1079, 1103, 1115
Ksp22I TGATCA 1 cut(s) 580
KspAI GTTAAC 1 cut(s) 795
Kzo9I GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
LmnI GCTCC 1 cut(s) 817
Lsp1109I GCAGC 1 cut(s) 765
LweI GCATC 4 cut(s) 35, 94, 583, 895
MaeI CTAG 5 cut(s) 57, 78, 867, 933, 939
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 1 cut(s) 857
MalI GATC 6 cut(s) 225, 415, 582, 1098, 1183, 1264
MboI GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
MboII GAAGA 7 cut(s) 437, 440, 698, 731, 1208, 1225, 1260
MflI RGATCY 2 cut(s) 413, 1181
MhlI GDGCHC 2 cut(s) 379, 814
MlyI GAGTC 3 cut(s) 155, 704, 1160
MnlI CCTC 6 cut(s) 57, 178, 317, 361, 1100, 1133
Mph1103I ATGCAT 1 cut(s) 512
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 5 cut(s) 84, 794, 827, 1173, 1227
MslI CAYNNNNRTG 4 cut(s) 115, 204, 588, 1080
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 1 cut(s) 681
MwoI GCNNNNNNNGC 1 cut(s) 980
NcoI CCATGG 1 cut(s) 521
NdeII GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
NlaIII CATG 9 cut(s) 209, 514, 525, 587, 607, 823, 1079, 1103, 1115
NlaIV GGNNCC 3 cut(s) 189, 519, 1183
NsiI ATGCAT 1 cut(s) 512
NspI RCATGY 2 cut(s) 209, 1079
PaqCI CACCTGC 1 cut(s) 761
PciI ACATGT 2 cut(s) 205, 1075
PcsI WCGNNNNNNNCGW 2 cut(s) 232, 645
PctI GAATGC 1 cut(s) 512
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 4, 88
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 1 cut(s) 780
PleI GAGTC 3 cut(s) 155, 704, 1159
PpsI GAGTC 3 cut(s) 155, 704, 1159
PscI ACATGT 2 cut(s) 205, 1075
Psp6I CCWGG 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 983
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 3 cut(s) 189, 519, 1183
PsuI RGATCY 2 cut(s) 413, 1181
RsaI GTAC 4 cut(s) 701, 740, 865, 890
RsaNI GTAC 4 cut(s) 700, 739, 864, 889
RseI CAYNNNNRTG 4 cut(s) 115, 204, 588, 1080
SalI GTCGAC 1 cut(s) 251
SaqAI TTAA 5 cut(s) 84, 794, 827, 1173, 1227
SatI GCNGC 1 cut(s) 779
Sau3AI GATC 6 cut(s) 223, 413, 580, 1096, 1181, 1262
SchI GAGTC 3 cut(s) 155, 704, 1160
ScrFI CCNGG 1 cut(s) 681
SduI GDGCHC 2 cut(s) 379, 814
SfaNI GCATC 4 cut(s) 35, 94, 583, 895
SmiMI CAYNNNNRTG 4 cut(s) 115, 204, 588, 1080
SmlI CTYRAG 1 cut(s) 1056
SmoI CTYRAG 1 cut(s) 1056
SsiI CCGC 2 cut(s) 60, 768
SspI AATATT 1 cut(s) 835
SspMI CTAG 5 cut(s) 57, 78, 867, 933, 939
StyD4I CCNGG 1 cut(s) 679
StyI CCWWGG 1 cut(s) 521
TaaI ACNGT 2 cut(s) 135, 863
TaiI ACGT 1 cut(s) 651
TaqI TCGA 6 cut(s) 164, 202, 226, 235, 252, 1136
TatI WGTACW 1 cut(s) 699
TfiI GAWTC 2 cut(s) 4, 88
Tru1I TTAA 5 cut(s) 84, 794, 827, 1173, 1227
Tru9I TTAA 5 cut(s) 84, 794, 827, 1173, 1227
TscAI CASTG 1 cut(s) 402
TseI GCWGC 1 cut(s) 778
TspDTI ATGAA 6 cut(s) 17, 573, 672, 1232, 1260, 1272
TspRI CASTG 1 cut(s) 402
Van91I CCANNNNNTGG 1 cut(s) 266
XapI RAATTY 2 cut(s) 733, 957
XbaI TCTAGA 1 cut(s) 77
XceI RCATGY 2 cut(s) 209, 1079
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XmiI GTMKAC 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 5 cut(s) 57, 78, 867, 933, 939
Zsp2I ATGCAT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.