RchiOBHm_Chr5g0053341

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000080
Physical Location & Seq
Reverse (-)
55589654 .. 55593194
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33062

Sequence Viewer

Length: 1623 bp
ATGGCAGCTGAAAATGCCAAGAGAGGGATAAAGAGGTTCTTAGAACCATCTACCGGTGGCAGAATTGATCAAGAAAAATCTGAATATGGTCATGTGAGACCAAAAAGATTGAAAAGAATGTTTCATCGAGAAATTGCTGGAGGATCTAATGAATGTCGAAATGAAATGCAATTGAAAGGTGATGATGAAAACGCTGCGGAAGGGATGGCAGCTAAGGATGCCAAGAAAGGAAAAAAAAGATTGTCAAAAGAATCCACTACTAAGAGAGTAAATCAAGGAAAAACTGAACGTAGTCATGTTAGATCAAAGAGATTGAAAAGAATGTTTTGCCGAGAGAGTAATGAAAAAGATAATCAATGTCAGAGTGAGACCGAATTCAGTGATCAACGTTCTCGACTATTTGGTCAAGCGTCATATCAACAAAACAGACGAGGGTTAACTGTGAAATACGAAGACTCGGGGGATAATGTGTACTCATGTAACCATTGTGATGCATGCTTTTGGCTTGGAGAAGCTACTAAAAGATCAACTTCAAATGCATCAATTATCTATACAAATTGTTGTAGGAAAGGCGAAATCAAACTTGAAAGATCAAAACCAACACCAACTTTCCTCGAGCATTTATTGAATCCAGACAATGGTTCAGAAAGCAGATCATTCAGAGAAAACATAAGAATTTATAATTCAATGTTTGCTTTTACATCAATGGGAGCAAATATTGACTACAAAATAAATAATGGTTTAGGTCCTTATGTTTTCAAAATTAGTGGTCAAGTACATCATTTAATGGGTTCTTTATTACCTTCTAATGGAGAATCTCCTAAATATGCTCAATTATATATATACGATACAAAAAATGAAGTTTCAAATCGCATTAATGCAATTGATCCGAATCACAATAATGTAAATATCAAATCAAATATAGTTGAAGGACTCATTAAAATGTTTGACCAAAATAATGAATTAGTAAAAGAATTTCGAACTATAAGAGATAGATATGAAAATGGTTTCATTCCTACATTAAATATGACTATACTTAATCGTCAACCTACTGATAGCAAACAATATGAAACACCAACCAGTGAAGAAATCGGTGGTTTAATTGTTGGTGAGATTGGACAATATAATGCAAATAAAGATTTAATTATTGAATCAAAAAGTGGTTGTTTACAACGTATTTCTAAAATACATCCAAAATACATGTCATTACAATATCCAATACTTTTTCCATATGGTGAAGATGGATATAAACCGAATTTACTAATAGAGCAAATACCTGGAAATTCTAAAAAAAAAAGACAACGAATATCTATGCGAGCATACATTGCGTATCAAATCCAAGACCGAAATAATGAAGTAAAAACTTTATTAAAAGGTGGACGTTTATTTCAACAATTTTTAGTAGATTCTTATGCAACAGTTGAAGAAGACGGTTTAGATTGGATTAGAAAAAATCAAAAGAATTTTCGTAGTGAAATATATAAAGAACTTTGTACTTCAGCTACAGCAGGAATTAATAAAGCATCCAATTTAGGACAAAAAATTATTCTACCGAGTTCTTATACTGGAAGTCCTAGAGATATGATTAATAATTATCAAGATGCTATGGCAATTTGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

540

Amino Acids

61.78

Weight (kDa)

9.34

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 441 - 540 1.7e-19 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
AasI GACNNNNNNGTC 1 cut(s) 402
AccB7I CCANNNNNTGG 1 cut(s) 638
AciI CCGC 1 cut(s) 197
AclI AACGTT 1 cut(s) 388
AclWI GGATC 2 cut(s) 151, 881
AcsI RAATTY 6 cut(s) 374, 675, 974, 1255, 1282, 1462
AcuI CTGAAG 1 cut(s) 1482
AfaI GTAC 3 cut(s) 473, 777, 1495
AfiI CCNNNNNNNGG 4 cut(s) 24, 53, 638, 809
AflIII ACRYGT 1 cut(s) 1200
AgeI ACCGGT 1 cut(s) 53
AjnI CCWGG 1 cut(s) 1276
AluBI AGCT 4 cut(s) 8, 212, 515, 1502
AluI AGCT 4 cut(s) 8, 212, 515, 1502
Alw26I GTCTC 2 cut(s) 91, 362
AlwI GGATC 2 cut(s) 151, 881
Ama87I CYCGRG 2 cut(s) 457, 614
ApeKI GCWGC 3 cut(s) 5, 194, 209
ApoI RAATTY 6 cut(s) 374, 675, 974, 1255, 1282, 1462
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AseI ATTAAT 3 cut(s) 876, 1515, 1587
AsiGI ACCGGT 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 746
AsuHPI GGTGA 3 cut(s) 191, 1121, 1247
AsuII TTCGAA 1 cut(s) 979
AvaI CYCGRG 2 cut(s) 457, 614
AvaII GGWCC 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 459, 1434
BbvI GCAGC 3 cut(s) 17, 181, 221
BccI CCATC 3 cut(s) 55, 199, 1235
BciT130I CCWGG 1 cut(s) 1278
BclI TGATCA 2 cut(s) 67, 382
BcoDI GTCTC 2 cut(s) 91, 362
BfaI CTAG 1 cut(s) 1575
BfmI CTRYAG 1 cut(s) 1503
BisI GCNGC 3 cut(s) 6, 195, 210
BlsI GCNGC 3 cut(s) 7, 196, 211
Bme1390I CCNGG 1 cut(s) 1278
Bme18I GGWCC 1 cut(s) 746
BmeT110I CYCGRG 2 cut(s) 457, 614
BmgT120I GGNCC 1 cut(s) 746
BmrFI CCNGG 1 cut(s) 1278
BmsI GCATC 5 cut(s) 208, 481, 548, 1532, 1591
BpiI GAAGAC 2 cut(s) 459, 1434
BpmI CTGGAG 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 979
BsaBI GATNNNNATC 1 cut(s) 891
BsaI GGTCTC 2 cut(s) 91, 362
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 53, 638, 809
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 1080, 1570
Bse3DI GCAATG 1 cut(s) 1323
Bse8I GATNNNNATC 1 cut(s) 891
BseBI CCWGG 1 cut(s) 1278
BseGI GGATG 4 cut(s) 210, 223, 1189, 1523
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 4 cut(s) 24, 53, 638, 809
BseMI GCAATG 1 cut(s) 1323
BseNI ACTGG 2 cut(s) 1080, 1570
BseXI GCAGC 3 cut(s) 17, 181, 221
BshTI ACCGGT 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 457, 614
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 4 cut(s) 24, 53, 638, 809
BsmAI GTCTC 2 cut(s) 91, 362
Bso31I GGTCTC 2 cut(s) 91, 362
BsoBI CYCGRG 2 cut(s) 457, 614
Bsp119I TTCGAA 1 cut(s) 979
Bsp143I GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
BspACI CCGC 1 cut(s) 197
BspPI GGATC 2 cut(s) 151, 881
BspT104I TTCGAA 1 cut(s) 979
BspTNI GGTCTC 2 cut(s) 91, 362
BsrDI GCAATG 1 cut(s) 1323
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 2 cut(s) 1080, 1570
BssAI RCCGGY 1 cut(s) 53
BssMI GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
Bst2UI CCWGG 1 cut(s) 1278
Bst4CI ACNGT 3 cut(s) 442, 1420, 1433
BstAPI GCANNNNNTGC 1 cut(s) 1325
BstBI TTCGAA 1 cut(s) 979
BstC8I GCNNGC 2 cut(s) 496, 1317
BstDEI CTNAG 3 cut(s) 40, 213, 261
BstF5I GGATG 4 cut(s) 210, 223, 1189, 1523
BstKTI GATC 8 cut(s) 70, 146, 305, 385, 527, 593, 656, 889
BstMAI GTCTC 2 cut(s) 91, 362
BstMBI GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
BstMWI GCNNNNNNNGC 3 cut(s) 14, 218, 1325
BstNI CCWGG 1 cut(s) 1278
BstNSI RCATGY 2 cut(s) 498, 1204
BstSCI CCNGG 1 cut(s) 1276
BstSFI CTRYAG 1 cut(s) 1503
BstV1I GCAGC 3 cut(s) 17, 181, 221
BstV2I GAAGAC 2 cut(s) 459, 1434
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 4 cut(s) 210, 223, 1189, 1523
BtsIMutI CAGTG 2 cut(s) 385, 1087
Cac8I GCNNGC 2 cut(s) 496, 1317
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 746
CseI GACGC 1 cut(s) 399
Csp6I GTAC 3 cut(s) 472, 776, 1494
CspAI ACCGGT 1 cut(s) 53
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 5 cut(s) 92, 296, 477, 495, 1201
CviJI RGCY 5 cut(s) 8, 212, 505, 515, 1502
CviKI_1 RGCY 5 cut(s) 8, 212, 505, 515, 1502
CviQI GTAC 3 cut(s) 472, 776, 1494
DdeI CTNAG 3 cut(s) 40, 213, 261
DpnI GATC 8 cut(s) 69, 145, 304, 384, 526, 592, 655, 888
DpnII GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
DrdI GACNNNNNNGTC 1 cut(s) 402
DseDI GACNNNNNNGTC 1 cut(s) 402
Eco31I GGTCTC 2 cut(s) 91, 362
Eco47I GGWCC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 1482
Eco88I CYCGRG 2 cut(s) 457, 614
EcoO109I RGGNCCY 1 cut(s) 746
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 1276
EcoT22I ATGCAT 2 cut(s) 496, 541
FaeI CATG 5 cut(s) 95, 299, 480, 498, 1204
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 514, 546
FatI CATG 5 cut(s) 91, 295, 476, 494, 1200
FauNDI CATATG 1 cut(s) 1231
FbaI TGATCA 2 cut(s) 67, 382
Fnu4HI GCNGC 3 cut(s) 6, 195, 210
FokI GGATG 4 cut(s) 217, 230, 1176, 1510
Fsp4HI GCNGC 3 cut(s) 6, 195, 210
FspBI CTAG 1 cut(s) 1575
GluI GCNGC 3 cut(s) 6, 195, 210
GsuI CTGGAG 1 cut(s) 159
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 399
Hin1II CATG 5 cut(s) 95, 299, 480, 498, 1204
HincII GTYRAC 2 cut(s) 438, 1046
HindII GTYRAC 2 cut(s) 438, 1046
HinfI GANTC 8 cut(s) 251, 455, 628, 815, 892, 933, 1151, 1406
HpaI GTTAAC 1 cut(s) 438
HpaII CCGG 1 cut(s) 54
HphI GGTGA 3 cut(s) 191, 1121, 1247
Hpy166II GTNNAC 5 cut(s) 438, 472, 1046, 1169, 1379
Hpy188I TCNGA 5 cut(s) 82, 363, 646, 662, 891
Hpy188III TCNNGA 5 cut(s) 71, 128, 393, 632, 1598
Hpy8I GTNNAC 5 cut(s) 438, 472, 1046, 1169, 1379
HpyAV CCTTC 3 cut(s) 194, 813, 923
HpyCH4III ACNGT 3 cut(s) 442, 1420, 1433
HpyCH4IV ACGT 4 cut(s) 289, 388, 1174, 1381
HpyCH4V TGCA 6 cut(s) 169, 494, 539, 881, 1130, 1415
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 218, 1325
HpyF3I CTNAG 3 cut(s) 40, 213, 261
HpySE526I ACGT 4 cut(s) 289, 388, 1174, 1381
Hsp92II CATG 5 cut(s) 95, 299, 480, 498, 1204
Ksp22I TGATCA 2 cut(s) 67, 382
KspAI GTTAAC 1 cut(s) 438
Kzo9I GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
LmnI GCTCC 1 cut(s) 710
LpnPI CCDG 8 cut(s) 67, 123, 645, 1093, 1263, 1290, 1494, 1551
Lsp1109I GCAGC 3 cut(s) 17, 181, 221
LweI GCATC 5 cut(s) 208, 481, 548, 1532, 1591
MaeI CTAG 1 cut(s) 1575
MaeII ACGT 4 cut(s) 289, 388, 1174, 1381
MaeIII GTNAC 1 cut(s) 479
MalI GATC 8 cut(s) 69, 145, 304, 384, 526, 592, 655, 888
MboI GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
MboII GAAGA 5 cut(s) 464, 1097, 1250, 1436, 1439
MfeI CAATTG 2 cut(s) 170, 882
MflI RGATCY 1 cut(s) 143
MlyI GAGTC 2 cut(s) 449, 927
MnlI CCTC 5 cut(s) 17, 27, 134, 425, 623
Mph1103I ATGCAT 2 cut(s) 496, 541
MslI CAYNNNNRTG 3 cut(s) 489, 900, 941
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 1278
MunI CAATTG 2 cut(s) 170, 882
MvaI CCWGG 1 cut(s) 1278
MwoI GCNNNNNNNGC 3 cut(s) 14, 218, 1325
NdeI CATATG 1 cut(s) 1231
NdeII GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
NlaIII CATG 5 cut(s) 95, 299, 480, 498, 1204
NmeAIII GCCGAG 1 cut(s) 356
NsiI ATGCAT 2 cut(s) 496, 541
NspI RCATGY 2 cut(s) 498, 1204
NspV TTCGAA 1 cut(s) 979
PaeI GCATGC 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 614
PciI ACATGT 1 cut(s) 1200
PfeI GAWTC 6 cut(s) 251, 628, 815, 892, 1151, 1406
PflMI CCANNNNNTGG 1 cut(s) 638
PinAI ACCGGT 1 cut(s) 53
PkrI GCNGC 3 cut(s) 7, 196, 211
PleI GAGTC 2 cut(s) 449, 927
PpsI GAGTC 2 cut(s) 449, 927
PpuMI RGGWCCY 1 cut(s) 746
PscI ACATGT 1 cut(s) 1200
PshBI ATTAAT 3 cut(s) 876, 1515, 1587
PsiI TTATAA 1 cut(s) 681
Psp1406I AACGTT 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 746
Psp6I CCWGG 1 cut(s) 1276
PspGI CCWGG 1 cut(s) 1276
PspPI GGNCC 1 cut(s) 746
PspPPI RGGWCCY 1 cut(s) 746
PspXI VCTCGAGB 1 cut(s) 614
PsuI RGATCY 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 3 cut(s) 473, 777, 1495
RsaNI GTAC 3 cut(s) 472, 776, 1494
RseI CAYNNNNRTG 3 cut(s) 489, 900, 941
SatI GCNGC 3 cut(s) 6, 195, 210
Sau3AI GATC 8 cut(s) 67, 143, 302, 382, 524, 590, 653, 886
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 2 cut(s) 449, 927
ScrFI CCNGG 1 cut(s) 1278
SfaNI GCATC 5 cut(s) 208, 481, 548, 1532, 1591
SfcI CTRYAG 1 cut(s) 1503
Sfr274I CTCGAG 1 cut(s) 614
SfuI TTCGAA 1 cut(s) 979
SinI GGWCC 1 cut(s) 746
SlaI CTCGAG 1 cut(s) 614
SmiMI CAYNNNNRTG 3 cut(s) 489, 900, 941
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SphI GCATGC 1 cut(s) 498
SsiI CCGC 1 cut(s) 197
SspI AATATT 1 cut(s) 718
SspMI CTAG 1 cut(s) 1575
StyD4I CCNGG 1 cut(s) 1276
TaaI ACNGT 3 cut(s) 442, 1420, 1433
TaiI ACGT 4 cut(s) 292, 391, 1177, 1384
TaqI TCGA 5 cut(s) 127, 157, 394, 615, 979
TaqII GACCGA 2 cut(s) 386, 1359
TatI WGTACW 3 cut(s) 471, 775, 1493
TfiI GAWTC 6 cut(s) 251, 628, 815, 892, 1151, 1406
TscAI CASTG 2 cut(s) 385, 1087
TseI GCWGC 3 cut(s) 5, 194, 209
TspRI CASTG 2 cut(s) 385, 1087
Van91I CCANNNNNTGG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 746
VspI ATTAAT 3 cut(s) 876, 1515, 1587
XapI RAATTY 6 cut(s) 374, 675, 974, 1255, 1282, 1462
XceI RCATGY 2 cut(s) 498, 1204
XhoI CTCGAG 1 cut(s) 614
XspI CTAG 1 cut(s) 1575
Zsp2I ATGCAT 2 cut(s) 496, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.