Rmu_sc0015523.1_g000009

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0015523.1
OG0000080
Physical Location & Seq
Forward (+)
26971 .. 39592
12622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0015523.1_g000009.1.cds

Sequence Viewer

Length: 5556 bp
atggcctttggtagtacaacccaatggagctcacattcggagctaggcgtttattcagtggttccttcctcggcaaccttcggtgcttcatcctcacgggctcttggaagtacaatgcctgcttatggttttggagcctcttataatcctgctttcagttgtttatggacaccatttgttgtgctagatgtgcaataccttcatgttaatgcagctattgattccagcataggccttaacccagcccctcttttagagttatcagcaacaattggaagtaaggatcttaggaaggttgatgaaattggaattcaagtccttattaggccacgtagtaatgttactgcgcaagcacgtggtagaggtcgcagacgcaggcgcgggcgcgaaactcataattgtgctagaaattttaatgaggccatcggagtcacaaaatttcagttgccagcatcgacgacatgtccacattgtcatgcccgtttattctatcacgaaagtagagacatgtgttgctccaatggaaagattgtcttgcctcccatacattctcctccagatatgatcgagctattctcctctgaatctccacaaggagcccattttaggcaaaacattcgagcctacaaccatgtattttcattcacatcaatgggtgtgcatgtcgatgaaagtcttgcaaccgggggtggtactttcacatttcgtgctcagggatccatatatcataagataggaagtcttctaccaaatgtcaatgatagaccaagatatctacaattgtatatatatgatactgaccatgaagtggacaatcgtatgctggagaatgcagcattacatagagatgtggttgaaaaaatacagagaattttgaatcaacataacccatttgtgcaaacatttcatcacctagctcagcgtccggatttgcaaaattgcagactcatcattagagagcaagctgcaaaccaacatcagtacagtttgccttcagcatcccacgttgctgcaataatagtgggtacaaatgatgtggaaaatctgacaggtcgtgatattgttgtgcaaacaaagcaaggccagcttctaaatattcgagattgtgttgggtactatgatcccttacagtatccattattgttcccatatggaacatatggatgggatgtgaatagtaggaataataatggtcaaaaattgacgtgtcgtgactattatgcgtatattttgcagatccgtcaatatgatgattcacttttactccgagggggccgtctattgcaacaatatgtcgtggataattacgtcaaaattgaaacacaaaagttgagatggattcgcagcaatcaaagtactcttcgacgtgagttgtatgacggactacatgactcacttaatgctggtgaaaataatgcaggtaacattggtcgcaagactattttaccatcatcttttattggaagtccacgtgatatgcaccaaagatatcaggatgcaatggctttagtccaaaaatatggaaaaccggatatttttctcacaatgacatgtaatccaaattgggaagaaataactgcagagttattacctggtcaagtggcacaagatcgcccggatctaactacgcgaatttttcacgccaaatttgaagagttaaagaatgatgttattaaaaaaggagtcttgggtactattgttgcatacgtatatgtcatcgagtttcaaaaacgtggtctcccacatgttcatatgcttctcatgttgagtgacgatgataaactaaacaacccagatgattatgaccacattgttcgggctgaaattccaaatcaaaatgaagagccagagttatacaatgcagtgttggggcacatgatacacggtccatgtggggtacataggcgaactgctccatgtatgaagcgtgggagctgcaaacgaggctatccaaagccattttcagtaaccacatttcaaggaaatgattcttatcctgtttatcgtagacgagataatcaatcagatatgcaatctaatggcaatcaaaatagaccactggataatagttgggttatcccctataatccatggttactcacaaaatatgattgtcatatcaatgtggaaatttgttgcagcattaagagcgtcaagtatttgtataaatatgtctacaaaggtccagacagagtaacatttgaagttcggcctgaagcaaatcaagatgaaataagaaactttgttgatgcaagatgggtttgtgcaccagaagctttgtggaggatgtttaaattcgtgatgaatcgaatgtatccatcagtggaacgattgcaaattcatcttccaaatcgacacaatgttttctttaatgccactgaaagtgtaacagcaattctggcaaatgaaagaagctcgatgacaatgctcacagaattttttactaagaatgcttattttgaaagtgcacgtcaatacttatatcgagagtttcctgagcattacaaatgggatgggagacttaaaacatgggaagcaaaggagaccaaccaaaaagtcattggaagaatttacacagtgtcaccttcagaaggagaaaagttttacttgcgtgttcttcttaatcatgttcgaggacctaagtcatttgaggatttaaggacagttgatggggtcttacaaccgacattcaagaaagcagttgaagaacgtggtttgttagaagatgatgaaagtattagacaatgtctacgtgaggcctctaatatacgtatgccgtcatctttaagaagattgtttgttaccatcctggtttattgcatgccccatggagtacgcagtttgtgggatgaattttatccattcatgatagaggattatccttcttcgagcaccacggacaatgtacgtatcacaaatagacttttacgggacttgaacgagttgttggtgcagcacagtaagtccgtctttgattatgacttgccagaaatgactcaagatggtggtgaaaattcttcaatgccaaggctaatacaagatgaaatttcaattaacattcctcaagaagaccgggatgcagttcatcatttgaatgaggatcaaacttttgcatacaattccataatatctgctattgagtgtcatgacaatgccatattttttgttgatggtccaggaggaactggaaagacatacttatatcgtgcattattagcaagcttgagaagcaatgaccatattgtattggcaacagcaacttctggcatagcagtaataatactacctggtgggaggacggcacactctaggtttaaaattcctcttaatcttgatgcatcttcaacatgttgtattagtaaacaatctgatttagcagagttgataagaaagtcatcggcaattgtttgggatgaagcaccaatgatgaatcgatatgggtttgaagctcttgatcgaacatttagagacataacaagtgtcgatttgccagttggaggaaaggtgatgatcttcgggggtgatttccgacaagttcttcctgttatccataagggtacgagatctgagatggtgcaagccagtttgattaatgcttcattttggaaggatgtgaagattcttcgtttgacacagaatatgagatccatcaatgatccatatttctcagagttcttacttcgtgttggtaatggagaacaaccaactgtggttgaagacataatacaaataccttggcccatgatcataccatgggagaatgaagaatcaattaaccagttggttaatcaagtcttccctaatttggatcgtcatgtaaatgatgccgggtacatggttgaaagagcaataattactcaaaaaaatgatgatgttgacgtactcaatgaaagaatcatacaacactttccagggcctgaacgaatcttatactctttcgactcagttgaggatgacaccagaaatatgtatcaacaagaatttttaaattcgatttcaccttctggtatgccgccacatcagttgattataaagaaaggtgccccaattatgcttttgaggaatattgacccaaagatggggttgtgcaacggtacaagattgacttgccgcggagcatataacaatctaattgatgcagagattttaacaggacatttttcgggaactagaattttcttgccaagaatctctcttaagagtgccgaaagttctagactcccgtttgagatgacaagaaagcagttccccgtaaagttgagttttgcacttaccataaataaatcacaaggccagacaataccaaatgttggtatttaccttcctgatcatgtgttcagccatggacaactttgcccggcttttgccactagtaacccagcttttgactttaccaccttttttggcgccccggttttcaacactgctagcactccagcttttggcactaattgtaccaccactttggccactacaggtaccccggcttttggcactactagtaccccaactattggcaccacgatcaccataacccctggtgtaggcacttctactgctccaactggtggcactaatgccatcgccataacctctagtgttggcactgctagtactccaactgttggcaatagtatcaccatagccccatctgttggcgctactgtcaccatagccccagctttgggtactagtatcaccataacccaagctgcgggaactactatcaccataaccagcaacccaacttatggtgctacaagcaccccaggcattggtggtgcaagtactccatcctttttctttccaacatccactgctcctacaagcaccccaggcggttttactaattcttttcgctttccaacatccactacttctacttttgcgcaatcaacttgcacctcttgcagcagccaagttggtgcttcatctccttttggaccgcggagacctccatttggagctcagtcaacaccaatagttgggaacaatgcctctaggcagtcagcttttaggggccaccacgggcagggaagtagagtggttccttacacagcatctgctgaaatagatagtaatggtgctggtcgtatgaagtcaatatcagcaatgccaattcacgagcataaatgtcacgaggaactgagatgggaagattatcaattgggggataaaggtggagcggttcctcctggtgggagtggcttcagctgccagtttaccacacagtcatttcttcagaaagaaaaacccacttcctttgggccgtatggaagtccatttgctcacacatcctcaagtacttctaatatctcaacttcatctccatttggtcaaccatcttcaagtccatttggtcaaccatcttcaaatccatatggtcgaacatcttcaagtccatttgctcaaccatcttcaagtcaatttgttcaaccatcttcaagtccatttgctcaaagcctcagttcatcaatttcggggaattccaatctttttacaccttcatcaattgggcaaacttcacctccacacgtaaatattttgtgtttataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1851

Amino Acids

206.0

Weight (kDa)

7.84

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 144, 4031, 5554
Acc16I TGCGCA 2 cut(s) 348, 4890
Acc36I ACCTGC 1 cut(s) 1409
Acc65I GGTACC 1 cut(s) 4478
AccB1I GGYRCC 4 cut(s) 4040, 4406, 4478, 4517
AccBSI CCGCTC 1 cut(s) 5186
AccI GTMKAC 3 cut(s) 2008, 2175, 2749
AccII CGCG 5 cut(s) 381, 387, 1630, 4113, 4948
AccIII TCCGGA 1 cut(s) 925
AciI CCGC 9 cut(s) 381, 4013, 4111, 4113, 4715, 4839, 4946, 4948, 5186
AcoI YGGCCR 1 cut(s) 4467
AcuI CTGAAG 5 cut(s) 978, 2235, 2571, 5194, 5225
AcvI CACGTG 2 cut(s) 356, 1472
AcyI GRCGYC 1 cut(s) 4407
AflII CTTAAG 1 cut(s) 4196
AflIII ACRYGT 7 cut(s) 461, 507, 1205, 1550, 1744, 3347, 5533
AhdI GACNNNNNGTC 1 cut(s) 462
AhlI ACTAGT 3 cut(s) 4370, 4499, 4691
AjiI CACGTC 3 cut(s) 1206, 1367, 2471
AjnI CCWGG 9 cut(s) 1591, 2808, 3175, 3286, 3910, 4537, 4768, 4834, 5194
AjuI GAANNNNNNNTTGG 4 cut(s) 1945, 1977, 2930, 2962
Alw21I GWGCWC 6 cut(s) 32, 712, 2269, 2470, 2894, 4969
Alw26I GTCTC 6 cut(s) 498, 1742, 2512, 2537, 3462, 4945
Alw44I GTGCAC 2 cut(s) 2265, 2466
AlwNI CAGNNNCTG 2 cut(s) 3917, 5063
Aor13HI TCCGGA 1 cut(s) 925
ApaLI GTGCAC 2 cut(s) 2265, 2466
ArsI GACNNNNNNTTYG 2 cut(s) 1720, 1752
AseI ATTAAT 1 cut(s) 3591
Asp700I GAANNNNTTC 2 cut(s) 1987, 5392
Asp718I GGTACC 1 cut(s) 4478
AspLEI GCGC 6 cut(s) 349, 381, 387, 4409, 4661, 4891
AsuC2I CCSGG 7 cut(s) 685, 1616, 3074, 3829, 4358, 4412, 4484
AsuNHI GCTAGC 1 cut(s) 4427
AvaII GGWCC 5 cut(s) 1886, 2183, 2636, 3173, 4943
BaeGI GKGCMC 4 cut(s) 1875, 2269, 2470, 4045
BaeI ACNNNNGTAYC 2 cut(s) 1683, 1716
BalI TGGCCA 1 cut(s) 4469
BamHI GGATCC 1 cut(s) 716
BanI GGYRCC 4 cut(s) 4040, 4406, 4478, 4517
BanII GRGCYC 4 cut(s) 32, 103, 601, 4969
BarI GAAGNNNNNNTAC 2 cut(s) 3708, 3740
BauI CACGAG 2 cut(s) 5123, 5138
BbrPI CACGTG 2 cut(s) 356, 1472
BbsI GAAGAC 4 cut(s) 734, 3075, 3723, 3787
Bbv12I GWGCWC 6 cut(s) 32, 712, 2269, 2470, 2894, 4969
BceAI ACGGC 4 cut(s) 1260, 2761, 3315, 5254
BcgI CGANNNNNNTGC 4 cut(s) 599, 633, 2407, 2441
BciT130I CCWGG 9 cut(s) 1593, 2810, 3177, 3288, 3912, 4539, 4770, 4836, 5196
BciVI GTATCC 2 cut(s) 1143, 2325
BclI TGATCA 2 cut(s) 3744, 4327
BcnI CCSGG 7 cut(s) 685, 1616, 3074, 3829, 4358, 4412, 4484
BcoDI GTCTC 6 cut(s) 498, 1742, 2512, 2537, 3462, 4945
BcuI ACTAGT 3 cut(s) 4370, 4499, 4691
BfmI CTRYAG 2 cut(s) 1578, 4473
BfoI RGCGCY 2 cut(s) 4410, 4662
BfrI CTTAAG 1 cut(s) 4196
BfuAI ACCTGC 1 cut(s) 1409
BfuI GTATCC 2 cut(s) 1143, 2325
BglII AGATCT 1 cut(s) 3563
BlpI GCTNAGC 1 cut(s) 918
BmcAI AGTACT 4 cut(s) 1357, 4615, 4789, 5305
Bme18I GGWCC 5 cut(s) 1886, 2183, 2636, 3173, 4943
BmeRI GACNNNNNGTC 1 cut(s) 462
BmgBI CACGTC 3 cut(s) 1206, 1367, 2471
BmtI GCTAGC 1 cut(s) 4431
BoxI GACNNNNGTC 1 cut(s) 2641
BpiI GAAGAC 4 cut(s) 734, 3075, 3723, 3787
BplI GAGNNNNNCTC 2 cut(s) 560, 592
BpmI CTGGAG 3 cut(s) 540, 845, 4419
Bpu10I CCTNAGC 2 cut(s) 711, 2496
Bpu1102I GCTNAGC 1 cut(s) 918
BpuEI CTTGAG 4 cut(s) 2982, 3048, 3244, 5284
BpuMI CCSGG 7 cut(s) 685, 1616, 3074, 3829, 4358, 4412, 4484
Bsa29I ATCGAT 1 cut(s) 3433
BsaAI YACGTR 8 cut(s) 332, 356, 1472, 1708, 2753, 2771, 2909, 5536
BsaBI GATNNNNATC 1 cut(s) 1992
BsaHI GRCGYC 1 cut(s) 4407
BsaI GGTCTC 3 cut(s) 1742, 2537, 4945
BsaWI WCCGGW 2 cut(s) 925, 1528
Bse1I ACTGG 7 cut(s) 2064, 3190, 3491, 3582, 3778, 4570, 5218
Bse3DI GCAATG 3 cut(s) 1506, 3238, 5118
Bse8I GATNNNNATC 1 cut(s) 1992
BseAI TCCGGA 1 cut(s) 925
BseBI CCWGG 9 cut(s) 1593, 2810, 3177, 3288, 3912, 4539, 4770, 4836, 5196
BseCI ATCGAT 1 cut(s) 3433
BseJI GATNNNNATC 1 cut(s) 1992
BseMI GCAATG 3 cut(s) 1506, 3238, 5118
BseMII CTCAG 9 cut(s) 725, 932, 2487, 3558, 3681, 3957, 4982, 5138, 5479
BseNI ACTGG 7 cut(s) 2064, 3190, 3491, 3582, 3778, 4570, 5218
BseRI GAGGAG 2 cut(s) 543, 568
BseSI GKGCMC 4 cut(s) 1875, 2269, 2470, 4045
BseYI CCCAGC 3 cut(s) 241, 4378, 4678
BsgI GTGCAG 1 cut(s) 2972
Bsh1236I CGCG 5 cut(s) 381, 387, 1630, 4113, 4948
BshNI GGYRCC 4 cut(s) 4040, 4406, 4478, 4517
BshVI ATCGAT 1 cut(s) 3433
BsiHKAI GWGCWC 6 cut(s) 32, 712, 2269, 2470, 2894, 4969
BsiSI CCGG 9 cut(s) 684, 926, 1529, 1616, 3073, 3828, 4358, 4412, 4484
BslFI GGGAC 1 cut(s) 2945
BsmAI GTCTC 6 cut(s) 498, 1742, 2512, 2537, 3462, 4945
BsmFI GGGAC 1 cut(s) 2945
BsmI GAATGC 2 cut(s) 835, 2455
Bso31I GGTCTC 3 cut(s) 1742, 2537, 4945
Bsp13I TCCGGA 1 cut(s) 925
Bsp1720I GCTNAGC 1 cut(s) 918
Bsp19I CCATGG 4 cut(s) 2090, 2827, 3752, 4342
BspACI CCGC 9 cut(s) 381, 4013, 4111, 4113, 4715, 4839, 4946, 4948, 5186
BspCNI CTCAG 9 cut(s) 724, 931, 2488, 3559, 3680, 3956, 4981, 5139, 5478
BspDI ATCGAT 1 cut(s) 3433
BspEI TCCGGA 1 cut(s) 925
BspFNI CGCG 5 cut(s) 381, 387, 1630, 4113, 4948
BspHI TCATGA 2 cut(s) 2865, 3145
BspMAI CTGCAG 1 cut(s) 1582
BspMI ACCTGC 1 cut(s) 1409
BspOI GCTAGC 1 cut(s) 4431
BspQI GCTCTTC 1 cut(s) 1836
BspT107I GGYRCC 4 cut(s) 4040, 4406, 4478, 4517
BspTI CTTAAG 1 cut(s) 4196
BspTNI GGTCTC 3 cut(s) 1742, 2537, 4945
BsrBI CCGCTC 1 cut(s) 5186
BsrDI GCAATG 3 cut(s) 1506, 3238, 5118
BsrI ACTGG 7 cut(s) 2064, 3190, 3491, 3582, 3778, 4570, 5218
BssNI GRCGYC 1 cut(s) 4407
BssSI CACGAG 2 cut(s) 5123, 5138
BssT1I CCWWGG 6 cut(s) 2090, 2827, 3026, 3734, 3752, 4342
Bst2BI CACGAG 2 cut(s) 5123, 5138
Bst2UI CCWGG 9 cut(s) 1593, 2810, 3177, 3288, 3912, 4539, 4770, 4836, 5196
Bst6I CTCTTC 3 cut(s) 1365, 1647, 1836
BstACI GRCGYC 1 cut(s) 4407
BstAFI CTTAAG 1 cut(s) 4196
BstAPI GCANNNNNTGC 1 cut(s) 4908
BstBAI YACGTR 8 cut(s) 332, 356, 1472, 1708, 2753, 2771, 2909, 5536
BstDSI CCRYGG 8 cut(s) 2090, 2827, 2895, 3752, 4111, 4342, 4946, 5026
BstENI CCTNNNNNAGG 2 cut(s) 2589, 4542
BstFNI CGCG 5 cut(s) 381, 387, 1630, 4113, 4948
BstH2I RGCGCY 2 cut(s) 4410, 4662
BstHHI GCGC 6 cut(s) 349, 381, 387, 4409, 4661, 4891
BstMAI GTCTC 6 cut(s) 498, 1742, 2512, 2537, 3462, 4945
BstNI CCWGG 9 cut(s) 1593, 2810, 3177, 3288, 3912, 4539, 4770, 4836, 5196
BstNSI RCATGY 7 cut(s) 465, 511, 665, 1554, 1748, 2824, 3351
BstPAI GACNNNNGTC 1 cut(s) 2641
BstSFI CTRYAG 2 cut(s) 1578, 4473
BstSLI GKGCMC 4 cut(s) 1875, 2269, 2470, 4045
BstSNI TACGTA 3 cut(s) 1708, 2771, 2909
BstUI CGCG 5 cut(s) 381, 387, 1630, 4113, 4948
BstV2I GAAGAC 4 cut(s) 734, 3075, 3723, 3787
BstX2I RGATCY 6 cut(s) 283, 716, 1236, 1618, 3563, 3644
BstXI CCANNNNNNTGG 2 cut(s) 1520, 4465
BstYI RGATCY 6 cut(s) 283, 716, 1236, 1618, 3563, 3644
Bsu15I ATCGAT 1 cut(s) 3433
BsuI GTATCC 2 cut(s) 1143, 2325
BsuTUI ATCGAT 1 cut(s) 3433
BtgI CCRYGG 8 cut(s) 2090, 2827, 2895, 3752, 4111, 4342, 4946, 5026
BtgZI GCGATG 1 cut(s) 4567
BtrI CACGTC 3 cut(s) 1206, 1367, 2471
BtsI GCAGTG 4 cut(s) 1869, 4422, 4605, 4815
BtsIMutI CAGTG 9 cut(s) 63, 1869, 2057, 2328, 2376, 2583, 4422, 4605, 4815
BveI ACCTGC 1 cut(s) 1409
CaiI CAGNNNCTG 2 cut(s) 3917, 5063
CciI TCATGA 2 cut(s) 2865, 3145
CfoI GCGC 6 cut(s) 349, 381, 387, 4409, 4661, 4891
Cfr42I CCGCGG 2 cut(s) 4114, 4949
ClaI ATCGAT 1 cut(s) 3433
CseI GACGC 3 cut(s) 381, 911, 2140
CsiI ACCWGGT 2 cut(s) 1591, 3286
DinI GGCGCC 1 cut(s) 4408
DraI TTTAAA 3 cut(s) 2293, 3316, 3987
DriI GACNNNNNGTC 1 cut(s) 462
EaeI YGGCCR 1 cut(s) 4467
Eam1104I CTCTTC 3 cut(s) 1365, 1647, 1836
Eam1105I GACNNNNNGTC 1 cut(s) 462
EarI CTCTTC 3 cut(s) 1365, 1647, 1836
Ecl136II GAGCTC 2 cut(s) 30, 4967
Eco105I TACGTA 3 cut(s) 1708, 2771, 2909
Eco130I CCWWGG 6 cut(s) 2090, 2827, 3026, 3734, 3752, 4342
Eco147I AGGCCT 2 cut(s) 234, 2759
Eco24I GRGCYC 4 cut(s) 32, 103, 601, 4969
Eco31I GGTCTC 3 cut(s) 1742, 2537, 4945
Eco32I GATATC 2 cut(s) 773, 1490
Eco47I GGWCC 5 cut(s) 1886, 2183, 2636, 3173, 4943
Eco53kI GAGCTC 2 cut(s) 30, 4967
Eco57I CTGAAG 5 cut(s) 978, 2235, 2571, 5194, 5225
Eco72I CACGTG 2 cut(s) 356, 1472
EcoICRI GAGCTC 2 cut(s) 30, 4967
EcoNI CCTNNNNNAGG 2 cut(s) 2589, 4542
EcoO109I RGGNCCY 2 cut(s) 2636, 3914
EcoRI GAATTC 2 cut(s) 309, 5485
EcoRII CCWGG 9 cut(s) 1591, 2808, 3175, 3286, 3910, 4537, 4768, 4834, 5194
EcoRV GATATC 2 cut(s) 773, 1490
EcoT14I CCWWGG 6 cut(s) 2090, 2827, 3026, 3734, 3752, 4342
EcoT22I ATGCAT 1 cut(s) 3340
EcoT38I GRGCYC 4 cut(s) 32, 103, 601, 4969
EgeI GGCGCC 1 cut(s) 4408
EheI GGCGCC 1 cut(s) 4408
ErhI CCWWGG 6 cut(s) 2090, 2827, 3026, 3734, 3752, 4342
FaqI GGGAC 1 cut(s) 2945
FauI CCCGC 2 cut(s) 374, 4708
FauNDI CATATG 4 cut(s) 1150, 1159, 1752, 5380
FbaI TGATCA 2 cut(s) 3744, 4327
FblI GTMKAC 3 cut(s) 2008, 2175, 2749
FriOI GRGCYC 4 cut(s) 32, 103, 601, 4969
FspI TGCGCA 2 cut(s) 348, 4890
GlaI GCGC 6 cut(s) 348, 380, 386, 4408, 4660, 4890
GsaI CCCAGC 3 cut(s) 245, 4382, 4682
GsuI CTGGAG 3 cut(s) 540, 845, 4419
HaeII RGCGCY 2 cut(s) 4410, 4662
HapII CCGG 9 cut(s) 684, 926, 1529, 1616, 3073, 3828, 4358, 4412, 4484
HgaI GACGC 3 cut(s) 381, 911, 2140
HhaI GCGC 6 cut(s) 349, 381, 387, 4409, 4661, 4891
Hin1I GRCGYC 1 cut(s) 4407
Hin6I GCGC 6 cut(s) 347, 379, 385, 4407, 4659, 4889
HinP1I GCGC 6 cut(s) 347, 379, 385, 4407, 4659, 4889
HincII GTYRAC 4 cut(s) 3877, 4974, 5339, 5363
HindII GTYRAC 4 cut(s) 3877, 4974, 5339, 5363
HindIII AAGCTT 2 cut(s) 2274, 3220
HpaII CCGG 9 cut(s) 684, 926, 1529, 1616, 3073, 3828, 4358, 4412, 4484
Hpy99I CGWCG 2 cut(s) 460, 1368
Hsp92I GRCGYC 1 cut(s) 4407
HspAI GCGC 6 cut(s) 347, 379, 385, 4407, 4659, 4889
KasI GGCGCC 1 cut(s) 4406
Kpn2I TCCGGA 1 cut(s) 925
KpnI GGTACC 1 cut(s) 4482
Ksp22I TGATCA 2 cut(s) 3744, 4327
KspI CCGCGG 2 cut(s) 4114, 4949
LguI GCTCTTC 1 cut(s) 1836
MabI ACCWGGT 2 cut(s) 1591, 3286
MbiI CCGCTC 1 cut(s) 5186
MfeI CAATTG 5 cut(s) 270, 779, 3402, 5165, 5511
MflI RGATCY 6 cut(s) 283, 716, 1236, 1618, 3563, 3644
MlsI TGGCCA 1 cut(s) 4469
MluNI TGGCCA 1 cut(s) 4469
Mly113I GGCGCC 1 cut(s) 4407
MlyI GAGTC 7 cut(s) 438, 939, 1385, 1692, 2989, 3935, 4212
MmeI TCCRAC 6 cut(s) 3475, 3553, 4586, 4643, 4832, 4889
Mox20I TGGCCA 1 cut(s) 4469
Mph1103I ATGCAT 1 cut(s) 3340
MroI TCCGGA 1 cut(s) 925
MroXI GAANNNNTTC 2 cut(s) 1987, 5392
MscI TGGCCA 1 cut(s) 4469
Msp20I TGGCCA 1 cut(s) 4469
MspA1I CMGCKG 3 cut(s) 4113, 4948, 5214
MspCI CTTAAG 1 cut(s) 4196
MspI CCGG 9 cut(s) 684, 926, 1529, 1616, 3073, 3828, 4358, 4412, 4484
MunI CAATTG 5 cut(s) 270, 779, 3402, 5165, 5511
Mva1269I GAATGC 2 cut(s) 835, 2455
MvaI CCWGG 9 cut(s) 1593, 2810, 3177, 3288, 3912, 4539, 4770, 4836, 5196
MvnI CGCG 5 cut(s) 381, 387, 1630, 4113, 4948
NarI GGCGCC 1 cut(s) 4407
NciI CCSGG 7 cut(s) 685, 1616, 3074, 3829, 4358, 4412, 4484
NcoI CCATGG 4 cut(s) 2090, 2827, 3752, 4342
NdeI CATATG 4 cut(s) 1150, 1159, 1752, 5380
NheI GCTAGC 1 cut(s) 4427
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 6 cut(s) 430, 1211, 1769, 2580, 4666, 5135
NsbI TGCGCA 2 cut(s) 348, 4890
NsiI ATGCAT 1 cut(s) 3340
NspI RCATGY 7 cut(s) 465, 511, 665, 1554, 1748, 2824, 3351
PaeI GCATGC 1 cut(s) 2824
PagI TCATGA 2 cut(s) 2865, 3145
PceI AGGCCT 2 cut(s) 234, 2759
PciI ACATGT 5 cut(s) 461, 507, 1550, 1744, 3347
PciSI GCTCTTC 1 cut(s) 1836
PctI GAATGC 2 cut(s) 835, 2455
PdmI GAANNNNTTC 2 cut(s) 1987, 5392
PflFI GACNNNGTC 1 cut(s) 2745
PfoI TCCNGGA 1 cut(s) 3175
PleI GAGTC 7 cut(s) 437, 939, 1385, 1691, 2989, 3935, 4212
PluTI GGCGCC 1 cut(s) 4410
PmaCI CACGTG 2 cut(s) 356, 1472
PmlI CACGTG 2 cut(s) 356, 1472
PpsI GAGTC 7 cut(s) 437, 939, 1385, 1691, 2989, 3935, 4212
Ppu21I YACGTR 8 cut(s) 332, 356, 1472, 1708, 2753, 2771, 2909, 5536
PpuMI RGGWCCY 1 cut(s) 2636
PscI ACATGT 5 cut(s) 461, 507, 1550, 1744, 3347
PshAI GACNNNNGTC 1 cut(s) 2641
PshBI ATTAAT 1 cut(s) 3591
PsiI TTATAA 3 cut(s) 144, 4031, 5554
Psp124BI GAGCTC 2 cut(s) 32, 4969
Psp5II RGGWCCY 1 cut(s) 2636
Psp6I CCWGG 9 cut(s) 1591, 2808, 3175, 3286, 3910, 4537, 4768, 4834, 5194
PspCI CACGTG 2 cut(s) 356, 1472
PspFI CCCAGC 3 cut(s) 241, 4378, 4678
PspGI CCWGG 9 cut(s) 1591, 2808, 3175, 3286, 3910, 4537, 4768, 4834, 5194
PspPPI RGGWCCY 1 cut(s) 2636
PstI CTGCAG 1 cut(s) 1582
PstNI CAGNNNCTG 2 cut(s) 3917, 5063
PsuI RGATCY 6 cut(s) 283, 716, 1236, 1618, 3563, 3644
PsyI GACNNNGTC 1 cut(s) 2745
PvuII CAGCTG 1 cut(s) 5214
SacI GAGCTC 2 cut(s) 32, 4969
SacII CCGCGG 2 cut(s) 4114, 4949
SapI GCTCTTC 1 cut(s) 1836
ScaI AGTACT 4 cut(s) 1357, 4615, 4789, 5305
SchI GAGTC 7 cut(s) 438, 939, 1385, 1692, 2989, 3935, 4212
SexAI ACCWGGT 2 cut(s) 1591, 3286
SfcI CTRYAG 2 cut(s) 1578, 4473
SfoI GGCGCC 1 cut(s) 4408
Sfr303I CCGCGG 2 cut(s) 4114, 4949
SgrBI CCGCGG 2 cut(s) 4114, 4949
SinI GGWCC 5 cut(s) 1886, 2183, 2636, 3173, 4943
SmlI CTYRAG 5 cut(s) 2997, 3063, 3223, 4196, 5299
SmoI CTYRAG 5 cut(s) 2997, 3063, 3223, 4196, 5299
SnaBI TACGTA 3 cut(s) 1708, 2771, 2909
SpeI ACTAGT 3 cut(s) 4370, 4499, 4691
SphI GCATGC 1 cut(s) 2824
SseBI AGGCCT 2 cut(s) 234, 2759
SsiI CCGC 9 cut(s) 381, 4013, 4111, 4113, 4715, 4839, 4946, 4948, 5186
SspDI GGCGCC 1 cut(s) 4406
SspI AATATT 3 cut(s) 1096, 4066, 5542
SstI GAGCTC 2 cut(s) 32, 4969
StuI AGGCCT 2 cut(s) 234, 2759
StyI CCWWGG 6 cut(s) 2090, 2827, 3026, 3734, 3752, 4342
TatI WGTACW 7 cut(s) 14, 110, 981, 1355, 4613, 4787, 5303
TauI GCSGC 2 cut(s) 4015, 4113
TscAI CASTG 9 cut(s) 63, 1869, 2064, 2328, 2383, 2583, 4429, 4612, 4822
TseFI GTSAC 6 cut(s) 430, 1211, 1769, 2580, 4666, 5135
Tsp45I GTSAC 6 cut(s) 430, 1211, 1769, 2580, 4666, 5135
TspGWI ACGGA 4 cut(s) 1229, 1395, 2912, 2956
TspRI CASTG 9 cut(s) 63, 1869, 2064, 2328, 2383, 2583, 4429, 4612, 4822
Tth111I GACNNNGTC 1 cut(s) 2745
Vha464I CTTAAG 1 cut(s) 4196
VneI GTGCAC 2 cut(s) 2265, 2466
VpaK11BI GGWCC 5 cut(s) 1886, 2183, 2636, 3173, 4943
VspI ATTAAT 1 cut(s) 3591
XagI CCTNNNNNAGG 2 cut(s) 2589, 4542
XbaI TCTAGA 1 cut(s) 4214
XceI RCATGY 7 cut(s) 465, 511, 665, 1554, 1748, 2824, 3351
XcmI CCANNNNNNNNNTGG 2 cut(s) 2277, 2558
XmiI GTMKAC 3 cut(s) 2008, 2175, 2749
XmnI GAANNNNTTC 2 cut(s) 1987, 5392
ZrmI AGTACT 4 cut(s) 1357, 4615, 4789, 5305
Zsp2I ATGCAT 1 cut(s) 3340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.