Rmu_sc0003441.1_g000022

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003441.1
OG0000080
Physical Location & Seq
Forward (+)
92598 .. 95720
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003441.1_g000022.1.cds

Sequence Viewer

Length: 2283 bp
atgggtgtgcatgtcgatgaaagtcttgcaaccgggggtggtactttcacatttcgtgctcagggatccatatatcataagataggaagtcttctaccaaatgctaatagaccaagatatctacaattgtacatatatgatactgaccatgaaatccgccaatatgatgattcacttttactccgcgggggtcgcctattgcaacaatatgtcgtggataattatgtcaaaattgaaacacaaaagttgagatggattcgtagcaatcaaagtactcttcgacgtgagttgtatgacggactacatgactcacttaatgctggtgaaaataatgcaggtaacattggtcacaagaccattttgccatcatcttttattggaagtccacgtgatatgcaccaaagatatcaggatgcaatggctttggtccaaaaatatggaaagccggattttttcctcacaatgacatgtaatccaaattgggaagaaataactgcagagttattacctggtcaagtggcacaagatcgcccggatctaactacgcgaatttttcacgccaaatttgaagagttaaagaatgatgttattaaaaaaggagtcttgggtactgttgtagcatacgtatacgtcatcgagtttcaaaaacgtggtctcccacacgttcatatgcttctcatgttaagtgacgatgataaactaaacaacccagatgattataaccacattgttcgggctgaaattccagatcaaaatgaagagccagagttatacaatgctgtgttggggcacatgatacacggtccatgtggggtacataggaaaactgctccatgtatgaagcgtgggagctgcaaacgaggctatccaaagccattttcagcaaacacatttcaaggaaatgattcttatcctgtttatcgtagtcgagacaatcactcagatatgcaatctcatggcaatcaaaatagaccactggataacagttgggttatcccctataacccatggttactcacaaaatatgattgtcatatcaatgtggaaatttgttgcagcattaagagtgtcaagtatttgtataaatctgtctataaaggtccagatagagtaacatttgaagttcgacctgaagcaaatcaagatgaaataagaaactttgttgatgcaagatgggtttgtgcaccagaagctttatggaggatgtttaaatttgtgatgaatcgaatatgggatgggagacataaaacatgggaagcaagggagaccaaccaaaaagtcattggaagaatttacacagtgtcaccttcagaaggagaaaagttttacttgcgtgttcttcttaatcatgttcgaggacctaagtcatttgaggatttaaggacatttgatggtgtcttacaaccgacattcaagaaagcagttgaacaacgtggtttgttagaagatgacgaaagtattagacaatgtctacttgaggcctctaatattcgtatgccgtcatctttaagaagattgtttgtcaccatcctagtttattgcatgccccatggtgtatgcagtttgtgggatgaattttatccattcatgatagaggattatccttcttcgagcaccacagacaatgtacgtatcacaaatagacttttacgagacttgaaccagttgctggtgcagcacagtaagtctgtctttgattatgacttgccagaaatgactcaagatggtggtgaaaattcttcaatgccgaggctaatacaagatgaaatttcaattaacattcctcaagaagactgtgatgcagttcatcatttgaatgaggatcaaacttttgcatacaattccataatatctgctattgagtgtcatgacaatgccatatatttcgttgatggtccaggaggaactagaaagacatacttatattgtgcattattagcaagcttgagaagtaatgatcatattgtattggcaacagcaacttctggcatagcagcaacaatactacctggtgggaggacagcacactctaggtttaaaattcctcttaatcttgatgcatcttcgacatgctccattagtaaacagtctgatttagcagagttgatcagaaagtcattggtaattatttgggatgaagcatcaatgatgaatcgatatgggtttgaagctcttgatcgaacatttagagacataacaagtgtcgatttgccatttggaggaaaggtgatgatctctcagttgaggatgacaccagaaatatgtatcaacaagaatttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

760

Amino Acids

87.11

Weight (kDa)

6.42

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
Acc36I ACCTGC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 1669
AccI GTMKAC 2 cut(s) 627, 1471
AccII CGCG 2 cut(s) 186, 547
AciI CCGC 3 cut(s) 157, 184, 186
AclWI GGATC 4 cut(s) 60, 73, 543, 1830
AcuI CTGAAG 2 cut(s) 1152, 1293
AcvI CACGTG 1 cut(s) 389
AfaI GTAC 6 cut(s) 43, 131, 274, 610, 816, 1629
AfiI CCNNNNNNNGG 3 cut(s) 1313, 1669, 2219
AflIII ACRYGT 2 cut(s) 467, 661
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 3 cut(s) 508, 1897, 2008
AjuI GAANNNNNNNTTGG 2 cut(s) 862, 894
AluBI AGCT 4 cut(s) 852, 1193, 1944, 2171
AluI AGCT 4 cut(s) 852, 1193, 1944, 2171
Alw21I GWGCWC 3 cut(s) 61, 1186, 1616
Alw26I GTCTC 6 cut(s) 659, 924, 1234, 1259, 1647, 2184
Alw44I GTGCAC 1 cut(s) 1182
AlwI GGATC 4 cut(s) 60, 73, 543, 1830
AlwNI CAGNNNCTG 1 cut(s) 1669
AoxI GGCC 1 cut(s) 1479
ApaLI GTGCAC 1 cut(s) 1182
ApeKI GCWGC 4 cut(s) 852, 1056, 1675, 1994
ArsI GACNNNNNNTTYG 2 cut(s) 637, 669
Asp700I GAANNNNTTC 1 cut(s) 904
AspS9I GGNCC 5 cut(s) 427, 803, 1100, 1358, 1895
AsuC2I CCSGG 2 cut(s) 34, 533
AsuHPI GGTGA 5 cut(s) 335, 1296, 1516, 1742, 2239
AvaII GGWCC 5 cut(s) 427, 803, 1100, 1358, 1895
BaeGI GKGCMC 2 cut(s) 792, 1186
BaeI ACNNNNGTAYC 2 cut(s) 600, 633
BamHI GGATCC 1 cut(s) 65
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 83, 1797
Bbv12I GWGCWC 3 cut(s) 61, 1186, 1616
BbvI GCAGC 4 cut(s) 839, 1068, 1687, 2006
BccI CCATC 8 cut(s) 246, 373, 1167, 1229, 1385, 1535, 1718, 1886
BceAI ACGGC 1 cut(s) 1483
BciT130I CCWGG 3 cut(s) 510, 1899, 2010
BclI TGATCA 2 cut(s) 1957, 2106
BcnI CCSGG 2 cut(s) 34, 533
BcoDI GTCTC 6 cut(s) 659, 924, 1234, 1259, 1647, 2184
BfaI CTAG 3 cut(s) 1532, 1908, 2031
BfmI CTRYAG 1 cut(s) 495
BfuAI ACCTGC 1 cut(s) 326
BisI GCNGC 4 cut(s) 853, 1057, 1676, 1995
BlsI GCNGC 4 cut(s) 854, 1058, 1677, 1996
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 5 cut(s) 34, 510, 533, 1899, 2010
Bme18I GGWCC 5 cut(s) 427, 803, 1100, 1358, 1895
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 5 cut(s) 427, 803, 1100, 1358, 1895
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 5 cut(s) 34, 510, 533, 1899, 2010
BmsI GCATC 6 cut(s) 403, 1156, 1789, 2047, 2069, 2150
BoxI GACNNNNGTC 1 cut(s) 1363
BpiI GAAGAC 2 cut(s) 83, 1797
Bpu10I CCTNAGC 1 cut(s) 60
BpuEI CTTGAG 4 cut(s) 1496, 1704, 1770, 1966
BpuMI CCSGG 2 cut(s) 34, 533
Bsa29I ATCGAT 1 cut(s) 2155
BsaAI YACGTR 3 cut(s) 389, 625, 1631
BsaBI GATNNNNATC 1 cut(s) 909
BsaI GGTCTC 2 cut(s) 659, 1259
BsaJI CCNNGG 5 cut(s) 33, 184, 1007, 1549, 1748
BsaXI ACNNNNNCTCC 6 cut(s) 165, 195, 639, 669, 2211, 2241
Bsc4I CCNNNNNNNGG 3 cut(s) 1313, 1669, 2219
Bse1I ACTGG 2 cut(s) 981, 1663
Bse3DI GCAATG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 909
BseBI CCWGG 3 cut(s) 510, 1899, 2010
BseCI ATCGAT 1 cut(s) 2155
BseDI CCNNGG 5 cut(s) 33, 184, 1007, 1549, 1748
BseGI GGATG 7 cut(s) 418, 1209, 1240, 1527, 1576, 2140, 2253
BseJI GATNNNNATC 1 cut(s) 909
BseLI CCNNNNNNNGG 3 cut(s) 1313, 1669, 2219
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 3 cut(s) 74, 954, 2252
BseNI ACTGG 2 cut(s) 981, 1663
BseSI GKGCMC 2 cut(s) 792, 1186
BseXI GCAGC 4 cut(s) 839, 1068, 1687, 2006
BsgI GTGCAG 1 cut(s) 1694
Bsh1236I CGCG 2 cut(s) 186, 547
BshFI GGCC 1 cut(s) 1481
BshVI ATCGAT 1 cut(s) 2155
BsiHKAI GWGCWC 3 cut(s) 61, 1186, 1616
BsiSI CCGG 3 cut(s) 33, 446, 533
BslI CCNNNNNNNGG 3 cut(s) 1313, 1669, 2219
BsmAI GTCTC 6 cut(s) 659, 924, 1234, 1259, 1647, 2184
BsnI GGCC 1 cut(s) 1481
Bso31I GGTCTC 2 cut(s) 659, 1259
Bsp1286I GDGCHC 4 cut(s) 61, 792, 1186, 1616
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
Bsp19I CCATGG 2 cut(s) 1007, 1549
BspACI CCGC 3 cut(s) 157, 184, 186
BspANI GGCC 1 cut(s) 1481
BspCNI CTCAG 3 cut(s) 73, 953, 2251
BspDI ATCGAT 1 cut(s) 2155
BspFNI CGCG 2 cut(s) 186, 547
BspHI TCATGA 2 cut(s) 1587, 1867
BspLI GGNNCC 1 cut(s) 67
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 4 cut(s) 60, 73, 543, 1830
BspQI GCTCTTC 1 cut(s) 753
BspTNI GGTCTC 2 cut(s) 659, 1259
BsrDI GCAATG 1 cut(s) 423
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 2 cut(s) 981, 1663
BssECI CCNNGG 5 cut(s) 33, 184, 1007, 1549, 1748
BssMI GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
BssNAI GTATAC 1 cut(s) 628
BssT1I CCWWGG 2 cut(s) 1007, 1549
Bst1107I GTATAC 1 cut(s) 628
Bst2UI CCWGG 3 cut(s) 510, 1899, 2010
Bst4CI ACNGT 7 cut(s) 613, 803, 986, 1300, 1682, 1796, 2088
Bst6I CTCTTC 3 cut(s) 282, 564, 753
BstAUI TGTACA 1 cut(s) 129
BstBAI YACGTR 3 cut(s) 389, 625, 1631
BstC8I GCNNGC 2 cut(s) 1544, 1942
BstDEI CTNAG 4 cut(s) 60, 940, 1362, 2238
BstDSI CCRYGG 3 cut(s) 184, 1007, 1549
BstENI CCTNNNNNAGG 1 cut(s) 1311
BstF5I GGATG 7 cut(s) 418, 1209, 1240, 1527, 1576, 2140, 2253
BstFNI CGCG 2 cut(s) 186, 547
BstKTI GATC 9 cut(s) 68, 529, 538, 751, 1825, 1960, 2109, 2179, 2235
BstMAI GTCTC 6 cut(s) 659, 924, 1234, 1259, 1647, 2184
BstMBI GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
BstMWI GCNNNNNNNGC 5 cut(s) 192, 861, 1190, 1675, 1937
BstNI CCWGG 3 cut(s) 510, 1899, 2010
BstNSI RCATGY 4 cut(s) 14, 471, 1546, 2073
BstPAI GACNNNNGTC 1 cut(s) 1363
BstSCI CCNGG 5 cut(s) 32, 508, 531, 1897, 2008
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 2 cut(s) 792, 1186
BstSNI TACGTA 2 cut(s) 625, 1631
BstUI CGCG 2 cut(s) 186, 547
BstV1I GCAGC 4 cut(s) 839, 1068, 1687, 2006
BstV2I GAAGAC 2 cut(s) 83, 1797
BstX2I RGATCY 2 cut(s) 65, 535
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 2 cut(s) 65, 535
BstZ17I GTATAC 1 cut(s) 628
Bsu15I ATCGAT 1 cut(s) 2155
BsuRI GGCC 1 cut(s) 1481
BsuTUI ATCGAT 1 cut(s) 2155
BtgI CCRYGG 3 cut(s) 184, 1007, 1549
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 7 cut(s) 418, 1209, 1240, 1527, 1576, 2140, 2253
BtsIMutI CAGTG 2 cut(s) 974, 1305
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 2 cut(s) 1544, 1942
CaiI CAGNNNCTG 1 cut(s) 1669
CciI TCATGA 2 cut(s) 1587, 1867
Cfr13I GGNCC 5 cut(s) 427, 803, 1100, 1358, 1895
Cfr42I CCGCGG 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 2155
CsiI ACCWGGT 2 cut(s) 508, 2008
Csp6I GTAC 6 cut(s) 42, 130, 273, 609, 815, 1628
CviQI GTAC 6 cut(s) 42, 130, 273, 609, 815, 1628
DdeI CTNAG 4 cut(s) 60, 940, 1362, 2238
DpnI GATC 9 cut(s) 67, 528, 537, 750, 1824, 1959, 2108, 2178, 2234
DpnII GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
DraI TTTAAA 2 cut(s) 1210, 2038
Eam1104I CTCTTC 3 cut(s) 282, 564, 753
EarI CTCTTC 3 cut(s) 282, 564, 753
EciI GGCGGA 1 cut(s) 146
Eco105I TACGTA 2 cut(s) 625, 1631
Eco130I CCWWGG 2 cut(s) 1007, 1549
Eco147I AGGCCT 1 cut(s) 1481
Eco31I GGTCTC 2 cut(s) 659, 1259
Eco32I GATATC 2 cut(s) 119, 407
Eco47I GGWCC 5 cut(s) 427, 803, 1100, 1358, 1895
Eco57I CTGAAG 2 cut(s) 1152, 1293
Eco72I CACGTG 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 1311
EcoO109I RGGNCCY 1 cut(s) 1358
EcoRII CCWGG 3 cut(s) 508, 1897, 2008
EcoRV GATATC 2 cut(s) 119, 407
EcoT14I CCWWGG 2 cut(s) 1007, 1549
EcoT22I ATGCAT 1 cut(s) 2062
ErhI CCWWGG 2 cut(s) 1007, 1549
FalI AAGNNNNNCTT 6 cut(s) 1313, 1345, 1676, 1708, 1904, 1936
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 1 cut(s) 669
FbaI TGATCA 2 cut(s) 1957, 2106
FblI GTMKAC 2 cut(s) 627, 1471
Fnu4HI GCNGC 4 cut(s) 853, 1057, 1676, 1995
FokI GGATG 7 cut(s) 425, 1216, 1247, 1514, 1583, 2147, 2260
Fsp4HI GCNGC 4 cut(s) 853, 1057, 1676, 1995
FspBI CTAG 3 cut(s) 1532, 1908, 2031
GluI GCNGC 4 cut(s) 853, 1057, 1676, 1995
HaeIII GGCC 1 cut(s) 1481
HapII CCGG 3 cut(s) 33, 446, 533
HindIII AAGCTT 2 cut(s) 1191, 1942
HinfI GANTC 8 cut(s) 170, 256, 308, 600, 905, 1222, 1717, 2152
HpaII CCGG 3 cut(s) 33, 446, 533
HphI GGTGA 5 cut(s) 335, 1296, 1516, 1742, 2239
Hpy166II GTNNAC 5 cut(s) 386, 628, 1184, 1472, 2084
Hpy188I TCNGA 4 cut(s) 943, 1312, 2092, 2111
Hpy8I GTNNAC 5 cut(s) 386, 628, 1184, 1472, 2084
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 3 cut(s) 1307, 1317, 1614
HpyCH4III ACNGT 7 cut(s) 613, 803, 986, 1300, 1682, 1796, 2088
HpyCH4IV ACGT 8 cut(s) 283, 388, 624, 630, 649, 663, 1432, 1630
HpyF10VI GCNNNNNNNGC 5 cut(s) 192, 861, 1190, 1675, 1937
HpyF3I CTNAG 4 cut(s) 60, 940, 1362, 2238
HpySE526I ACGT 8 cut(s) 283, 388, 624, 630, 649, 663, 1432, 1630
Ksp22I TGATCA 2 cut(s) 1957, 2106
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 3 cut(s) 835, 849, 2078
Lsp1109I GCAGC 4 cut(s) 839, 1068, 1687, 2006
LweI GCATC 6 cut(s) 403, 1156, 1789, 2047, 2069, 2150
MabI ACCWGGT 2 cut(s) 508, 2008
MaeI CTAG 3 cut(s) 1532, 1908, 2031
MaeII ACGT 8 cut(s) 283, 388, 624, 630, 649, 663, 1432, 1630
MaeIII GTNAC 7 cut(s) 338, 347, 686, 1011, 1111, 1302, 1522
MalI GATC 9 cut(s) 67, 528, 537, 750, 1824, 1959, 2108, 2178, 2234
MboI GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
MfeI CAATTG 1 cut(s) 125
MflI RGATCY 2 cut(s) 65, 535
MhlI GDGCHC 4 cut(s) 61, 792, 1186, 1616
MlyI GAGTC 3 cut(s) 302, 609, 1711
Mph1103I ATGCAT 1 cut(s) 2062
MroXI GAANNNNTTC 1 cut(s) 904
MslI CAYNNNNRTG 4 cut(s) 15, 1038, 1815, 1872
MspA1I CMGCKG 1 cut(s) 186
MspI CCGG 3 cut(s) 33, 446, 533
MspR9I CCNGG 5 cut(s) 34, 510, 533, 1899, 2010
MunI CAATTG 1 cut(s) 125
MvaI CCWGG 3 cut(s) 510, 1899, 2010
MvnI CGCG 2 cut(s) 186, 547
MwoI GCNNNNNNNGC 5 cut(s) 192, 861, 1190, 1675, 1937
NciI CCSGG 2 cut(s) 34, 533
NcoI CCATGG 2 cut(s) 1007, 1549
NdeI CATATG 1 cut(s) 669
NdeII GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
NlaIV GGNNCC 1 cut(s) 67
NmeAIII GCCGAG 1 cut(s) 1773
NmuCI GTSAC 4 cut(s) 347, 686, 1302, 1522
NsiI ATGCAT 1 cut(s) 2062
NspI RCATGY 4 cut(s) 14, 471, 1546, 2073
PaeI GCATGC 1 cut(s) 1546
PagI TCATGA 2 cut(s) 1587, 1867
PceI AGGCCT 1 cut(s) 1481
PciI ACATGT 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 753
PdmI GAANNNNTTC 1 cut(s) 904
PfeI GAWTC 5 cut(s) 170, 256, 905, 1222, 2152
PflFI GACNNNGTC 1 cut(s) 1467
PflMI CCANNNNNTGG 1 cut(s) 1669
PfoI TCCNGGA 1 cut(s) 1897
PkrI GCNGC 4 cut(s) 854, 1058, 1677, 1996
PleI GAGTC 3 cut(s) 302, 608, 1711
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 3 cut(s) 302, 608, 1711
Ppu21I YACGTR 3 cut(s) 389, 625, 1631
PpuMI RGGWCCY 1 cut(s) 1358
PscI ACATGT 1 cut(s) 467
PshAI GACNNNNGTC 1 cut(s) 1363
PsiI TTATAA 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 1358
Psp6I CCWGG 3 cut(s) 508, 1897, 2008
PspCI CACGTG 1 cut(s) 389
PspGI CCWGG 3 cut(s) 508, 1897, 2008
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 5 cut(s) 427, 803, 1100, 1358, 1895
PspPPI RGGWCCY 1 cut(s) 1358
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 1669
PsuI RGATCY 2 cut(s) 65, 535
PsyI GACNNNGTC 1 cut(s) 1467
RsaI GTAC 6 cut(s) 43, 131, 274, 610, 816, 1629
RsaNI GTAC 6 cut(s) 42, 130, 273, 609, 815, 1628
RseI CAYNNNNRTG 4 cut(s) 15, 1038, 1815, 1872
SacII CCGCGG 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 753
SatI GCNGC 4 cut(s) 853, 1057, 1676, 1995
Sau3AI GATC 9 cut(s) 65, 526, 535, 748, 1822, 1957, 2106, 2176, 2232
Sau96I GGNCC 5 cut(s) 427, 803, 1100, 1358, 1895
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 3 cut(s) 302, 609, 1711
ScrFI CCNGG 5 cut(s) 34, 510, 533, 1899, 2010
SduI GDGCHC 4 cut(s) 61, 792, 1186, 1616
SexAI ACCWGGT 2 cut(s) 508, 2008
SfaNI GCATC 6 cut(s) 403, 1156, 1789, 2047, 2069, 2150
SfcI CTRYAG 1 cut(s) 495
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SinI GGWCC 5 cut(s) 427, 803, 1100, 1358, 1895
SmiMI CAYNNNNRTG 4 cut(s) 15, 1038, 1815, 1872
SmlI CTYRAG 4 cut(s) 1475, 1719, 1785, 1945
SmoI CTYRAG 4 cut(s) 1475, 1719, 1785, 1945
SnaBI TACGTA 2 cut(s) 625, 1631
SphI GCATGC 1 cut(s) 1546
SseBI AGGCCT 1 cut(s) 1481
SsiI CCGC 3 cut(s) 157, 184, 186
SspI AATATT 1 cut(s) 1489
SspMI CTAG 3 cut(s) 1532, 1908, 2031
StuI AGGCCT 1 cut(s) 1481
StyD4I CCNGG 5 cut(s) 32, 508, 531, 1897, 2008
StyI CCWWGG 2 cut(s) 1007, 1549
TaaI ACNGT 7 cut(s) 613, 803, 986, 1300, 1682, 1796, 2088
TaiI ACGT 8 cut(s) 286, 391, 627, 633, 652, 666, 1435, 1633
TatI WGTACW 2 cut(s) 129, 272
TfiI GAWTC 5 cut(s) 170, 256, 905, 1222, 2152
TscAI CASTG 2 cut(s) 981, 1305
TseFI GTSAC 4 cut(s) 347, 686, 1302, 1522
TseI GCWGC 4 cut(s) 852, 1056, 1675, 1994
Tsp45I GTSAC 4 cut(s) 347, 686, 1302, 1522
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 2 cut(s) 981, 1305
Tth111I GACNNNGTC 1 cut(s) 1467
Van91I CCANNNNNTGG 1 cut(s) 1669
VneI GTGCAC 1 cut(s) 1182
VpaK11BI GGWCC 5 cut(s) 427, 803, 1100, 1358, 1895
XagI CCTNNNNNAGG 1 cut(s) 1311
XceI RCATGY 4 cut(s) 14, 471, 1546, 2073
XcmI CCANNNNNNNNNTGG 2 cut(s) 1194, 1280
XmiI GTMKAC 2 cut(s) 627, 1471
XmnI GAANNNNTTC 1 cut(s) 904
XspI CTAG 3 cut(s) 1532, 1908, 2031
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 2062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.