Rw1G014140

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000080
Physical Location & Seq
Reverse (-)
31509379 .. 31514219
4841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014140.1

Sequence Viewer

Length: 3408 bp
ATGAGTTCAGAACAACGTGATGAGTATTTAGCACGACTACGAGCTAATTATAATTATCATCGCGGGCTAACATTAAATCATGAGCATAATATCCAAGTTCCGACTAGAATCCGCTTAAGTCATATAAGACAATTAGCACGGTCAAATAGGTCACAAGTGCCTGATATTGGAGCGAATCAAGACGATATCAACAATGGTGATCATATAGCAGTCAATGAAGAAATCTCAGAAAATGTCATTCCTCAAAATAACATGCCCTATAACAACGGTGTTGTGCCAGTACGTTTCAGAACTAGAGTGGGTGCAGATGATGCAGAAAATCTGAGAGGTCACTGTGCGGGCTACTATGATCCTTTACAATATCCATTGCTTTTACCTTATGGCACATATGGATGGGATGTGAATAGTCAGAATAATAATGGTACAAAATTGACGTGTCGTGACTATTACGCATATATTTTGCAGATTCGTCCAGATGATGAATCAATTTTACTTCGAGGAGGCCGCCTGTTACAACAATATGTCGTTGATAATTATGTGAAAATTGAGACACAAAAGCTTAGATGGATTCGTAACATTGGTCGCAGAACTATTTTACCTTCCTCATTTATCGGTAGTCCACGTGATATGCACCAGAGATACCAAGATGCAATGGCTTTGGTCCAAAAATACGGGAAGCCAGATATTTTCCTCACAATGACATGCAACCCGAATTGGGAAGAAATAAAGGCAGAGTTATTACCGGGACAAGTGGCACAAGATCGCCCTGATTTATTGAAGGAAGATGTTATCAAAAAAGGGGTCTTAGGTACTGTTGTTGCACATGTTTATGTCATCGAATTTCAAAAACGTGGTCTCCCACATGTTCATATGCTTCTCATGTTAAATGACGATGATAAGTTGAATAACCCGAATGATTATGACAAAATTGTTCGAGCTGAAATACCAAGTCAAGATGAAGAACCGGAGTTGTACAATGCCGTATTGAGGCACATGATACATGGTCCATATGGAGCACATAAGCGTAATGCCCCATGTATGAAGCGTGGGAGTTGTAAACGAGGTTATCCTAAGCCCTTTTCAGCCAACACATTTCAAGGGAATGACTCTTATCCCATTTACCGTAGGCGAGATGTTCACCCAAATGCAGAATCTGATTTCAATCAAAGTAGACAAGTAGATAACAGTTGGGTTATTCCGTACAACCCTTGGCTACTCACAAAATATGATTGTCATATCAATGTCGAAGTCTGTTGCAGCATTAAGAGTGTTAAGTATTTGTACAAATATGTCTACAAAGGTCCAGATAGAGTAACATTTGAAGTTCGACCCGAAGCTAATGAAGATGAAATAAGGAATTTTGTTGATGCAAGATGGGTTTGTGCACTAGAGGCTTTGTGGAGAATATTCAAATTTGTAATGAATCGTATGTATCCTTCAGTGGAACGATTGCAAATTCATCTTCCAAATAGACAGAGTGTTTTCTTTAATGCCAATGAAAGGGTAACAGATGTTCTAGCAAATGATAGAAACTCAATGATGATGCTCACAGAAATATATACGGTATCTCCTTCAGAAGGAGAAAAGTTCTACTTACGTGTTCTTTTGAATCATGTTAGAGGGCCTACATCATACGAGGATTTGAGGACAGTAGATGGCATCTTGCAACCAACATTTAAGAAAGCGGCTGAACAAAGAGGTCTGTTAGAAGACGATGATCGTATCCGACAATGTTTACATGAGGCTTCAAGCATACATATGCCTTCATCTTTAAGGAGATTGTTTGTCACTATCTTGGTTTATTGCAAGCCTCATGGGGTACGAAGTTTATGGGATGAATTTTACCAATTTATGATAGAAGATTATGCTTCTTCAAGCACTGCAGTCAGTGCACGTATCACAAACAGACTCTTACGAGACTTAAACGGATTGCTGGTGCAACATAGTAAATCTGTTTGTGATTATGATTTGCCTGAAATGGATGAAAATTATGCTGAAGATTCGTCAGTGCCTAGGCTAATACAGGATGAAGTTTCAATTATCATTCCTCAAGAAGACTGCGATGCAGTTCATCTCTTGAATGAGGATCAGTCATTTGCATACAATGCAATAATATCAGCTATAGAGTGCAATGACAATGCCATATTTTTCGTTGATGGTCCAGGAGGGACTGGAAAAACATACTTATATCGTGCACTTTTAGCAACCTTGAGAAGCAATGATCATATTGTACTGGCAACAGCAACTTCTGGAATTGCAGCAACAATAATGCCTGGTGGGAGAACGGCACACTCCAGGTTTAAAATTCCACTCAATCTTGATGCATCTTCTACATGCTCTATTAGTAAACAATCTAATTTAGCAGAGTTGATACGAAGATCATCAGCGATTATTTGTGATGAGGCACCAATGATGCATAGATTTTCATTTGAAGCTCTTGATCGGACTTTTAAAGACATAATAGGTGTTGATTTGCCATTTGGGGGCAAAGTGATTATTTTTGGGGGAGATTTCCGACAAGTTCTGCCCGTTGTCCATAAGGGTACAAGGTCTCAGATGGTCCAAGCCAGTCTGGTTAACTCTTCATTTTGGAAAGATGTGAGGATTCTTCGTTTGCGGCAAAATATGAGATCTATCAATGATCCAGAGTTCTCAGAGTTTTTACTTAATGTTGGTAATGGAGAACAACCATCTGTGGTTGAACACATGATACGATTACCGTCGTGTATGGCCATACCATGGGAGGGTGAAGAGTCAATCAACCGATTAGTTAATGAAGTTTTTCCTAATTTGGTGGCTCATGTAAATGATGCAGCGTACATGGTTGAAAGGGCAATAATCACTCCAAAGAATGATGATGTTGACGTGCTTAATGAGATGATTATAAAGCAATTTCCAGGGCCTGAACGGATCCTGTACTCTTTCGACTCAGTTGAGGATGACACCAGAAATATGTATCAACAAGAATTCTTAAATTCAATTTCACCTTCTGGTATGCCCCCACATCAGTTAACTATAAAAAAAGGTGCTCCCATTATGCTTTTGAGAAATATTGACCCTACGATGGGATTGTGCAATGGTACACGATTGACTTGCCGTGGATCATATAACAACCTTATTGACGCAGAGATTTTAACTGGTCAATTTGTTGGAACTAGAGTATTCTTACCAAGAATATCTCTAAAGAGTGCCGAGAGTTCTGGACTCCCTTTTGAGATGACAAGAAAACAATTCCCTGTAAAGTTGAGTTTTGCACTTACTATAAATAAATCACAAGGGCAAACGATACCGAATGTTGGCATCTACCTTCCAGATCATGTATTCAGTCATGGGCAGTTTATATGTCGCTTTATCACGAGGAATTTCAGAGGCTACCACCAAAGTGTTAGTCAAGAAAGGCTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1135

Amino Acids

129.88

Weight (kDa)

6.56

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 191 - 293 9.1e-37 Helitron helicase-like domain at N-terminus
PIF1 PF05970 693 - 898 1.6e-74 PIF1-like helicase
AAA_30 PF13604 693 - 897 1.1e-06 AAA domain
Pif1_2B_dom PF21530 989 - 1031 5.9e-20 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 51, 2885
AccB1I GGYRCC 1 cut(s) 2404
AccB7I CCANNNNNTGG 1 cut(s) 2739
AccI GTMKAC 2 cut(s) 1171, 1293
AccII CGCG 1 cut(s) 63
AciI CCGC 6 cut(s) 63, 112, 338, 505, 1685, 2617
AclWI GGATC 6 cut(s) 344, 2094, 2636, 2905, 2918, 3109
AcoI YGGCCR 1 cut(s) 2730
AcsI RAATTY 9 cut(s) 839, 1357, 1412, 1455, 1838, 2304, 2966, 2974, 3361
AcuI CTGAAG 3 cut(s) 1422, 1557, 2016
AcvI CACGTG 1 cut(s) 623
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 4 cut(s) 434, 823, 862, 1597
AjiI CACGTC 2 cut(s) 435, 2866
AjnI CCWGG 4 cut(s) 2161, 2272, 2294, 2896
AleI CACNNNNGTG 1 cut(s) 3381
AluBI AGCT 6 cut(s) 44, 559, 938, 1337, 2120, 2435
AluI AGCT 6 cut(s) 44, 559, 938, 1337, 2120, 2435
Alw21I GWGCWC 5 cut(s) 1018, 1387, 1894, 2197, 3031
Alw26I GTCTC 4 cut(s) 542, 860, 1911, 2556
Alw44I GTGCAC 3 cut(s) 1383, 1890, 2193
AlwI GGATC 6 cut(s) 344, 2094, 2636, 2905, 2918, 3109
AlwNI CAGNNNCTG 2 cut(s) 1154, 2903
AoxI GGCC 4 cut(s) 502, 1622, 2730, 2900
ApaLI GTGCAC 3 cut(s) 1383, 1890, 2193
ApeKI GCWGC 3 cut(s) 1257, 2258, 2813
ApoI RAATTY 9 cut(s) 839, 1357, 1412, 1455, 1838, 2304, 2966, 2974, 3361
ArsI GACNNNNNNTTYG 6 cut(s) 786, 818, 838, 870, 3373, 3405
Asp700I GAANNNNTTC 1 cut(s) 2781
AspA2I CCTAGG 1 cut(s) 2012
AspS9I GGNCC 7 cut(s) 661, 1004, 1301, 1622, 2159, 2560, 2900
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 4 cut(s) 209, 1130, 2759, 2976
AvaII GGWCC 5 cut(s) 661, 1004, 1301, 2159, 2560
AvrII CCTAGG 1 cut(s) 2012
BaeGI GKGCMC 3 cut(s) 1387, 1894, 2197
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BalI TGGCCA 1 cut(s) 2732
BamHI GGATCC 1 cut(s) 2910
BanI GGYRCC 1 cut(s) 2404
BarI GAAGNNNNNNTAC 2 cut(s) 1420, 1452
BauI CACGAG 1 cut(s) 3355
BbrPI CACGTG 1 cut(s) 623
BbsI GAAGAC 2 cut(s) 1716, 2061
Bbv12I GWGCWC 5 cut(s) 1018, 1387, 1894, 2197, 3031
BbvI GCAGC 3 cut(s) 1269, 2270, 2825
BccI CCATC 8 cut(s) 387, 558, 1368, 1649, 2150, 2551, 2698, 3058
BceAI ACGGC 3 cut(s) 965, 2301, 3081
BcgI CGANNNNNNTGC 2 cut(s) 3075, 3109
BciT130I CCWGG 4 cut(s) 2163, 2274, 2296, 2898
BciVI GTATCC 2 cut(s) 1443, 1733
BclI TGATCA 2 cut(s) 199, 2221
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 4 cut(s) 542, 860, 1911, 2556
BfaI CTAG 6 cut(s) 105, 294, 1388, 1517, 2013, 3156
BfmI CTRYAG 2 cut(s) 1881, 2121
BfrI CTTAAG 1 cut(s) 115
BfuI GTATCC 2 cut(s) 1443, 1733
BglII AGATCT 1 cut(s) 2630
BisI GCNGC 6 cut(s) 505, 1258, 1686, 2259, 2618, 2814
BlnI CCTAGG 1 cut(s) 2012
BlsI GCNGC 6 cut(s) 506, 1259, 1687, 2260, 2619, 2815
Bme1390I CCNGG 5 cut(s) 744, 2163, 2274, 2296, 2898
Bme18I GGWCC 5 cut(s) 661, 1004, 1301, 2159, 2560
BmgBI CACGTC 2 cut(s) 435, 2866
BmgT120I GGNCC 7 cut(s) 661, 1004, 1301, 1622, 2159, 2560, 2900
BmiI GGNNCC 2 cut(s) 2406, 2912
BmrFI CCNGG 5 cut(s) 744, 2163, 2274, 2296, 2898
BpiI GAAGAC 2 cut(s) 1716, 2061
BpmI CTGGAG 1 cut(s) 2278
Bpu10I CCTNAGC 1 cut(s) 1071
BpuEI CTTGAG 2 cut(s) 2034, 2230
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 3 cut(s) 623, 1598, 1895
BsaBI GATNNNNATC 1 cut(s) 1161
BsaI GGTCTC 2 cut(s) 860, 2556
BsaJI CCNNGG 5 cut(s) 1208, 2012, 2738, 2897, 3097
BsaWI WCCGGW 1 cut(s) 964
BsaXI ACNNNNNCTCC 4 cut(s) 840, 870, 1552, 1582
Bse1I ACTGG 5 cut(s) 278, 2176, 2238, 2568, 3142
Bse3DI GCAATG 5 cut(s) 365, 657, 2137, 2224, 3082
Bse8I GATNNNNATC 1 cut(s) 1161
BseBI CCWGG 4 cut(s) 2163, 2274, 2296, 2898
BseDI CCNNGG 5 cut(s) 1208, 2012, 2738, 2897, 3097
BseGI GGATG 6 cut(s) 398, 403, 1840, 1987, 2032, 2944
BseJI GATNNNNATC 1 cut(s) 1161
BseMI GCAATG 5 cut(s) 365, 657, 2137, 2224, 3082
BseMII CTCAG 5 cut(s) 240, 314, 2567, 2667, 2943
BseNI ACTGG 5 cut(s) 278, 2176, 2238, 2568, 3142
BseRI GAGGAG 1 cut(s) 513
BseSI GKGCMC 3 cut(s) 1387, 1894, 2197
BseXI GCAGC 3 cut(s) 1269, 2270, 2825
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 504, 1624, 2732, 2902
BshNI GGYRCC 1 cut(s) 2404
BsiHKAI GWGCWC 5 cut(s) 1018, 1387, 1894, 2197, 3031
BsiSI CCGG 2 cut(s) 743, 965
BslFI GGGAC 2 cut(s) 759, 2182
BsmAI GTCTC 4 cut(s) 542, 860, 1911, 2556
BsmFI GGGAC 2 cut(s) 759, 2182
BsnI GGCC 4 cut(s) 504, 1624, 2732, 2902
Bso31I GGTCTC 2 cut(s) 860, 2556
Bsp1286I GDGCHC 5 cut(s) 1018, 1387, 1894, 2197, 3031
Bsp1407I TGTACA 2 cut(s) 972, 1281
Bsp19I CCATGG 1 cut(s) 2738
BspACI CCGC 6 cut(s) 63, 112, 338, 505, 1685, 2617
BspANI GGCC 4 cut(s) 504, 1624, 2732, 2902
BspCNI CTCAG 5 cut(s) 239, 315, 2566, 2666, 2942
BspFNI CGCG 1 cut(s) 63
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 2406, 2912
BspMAI CTGCAG 1 cut(s) 1885
BspPI GGATC 6 cut(s) 344, 2094, 2636, 2905, 2918, 3109
BspT107I GGYRCC 1 cut(s) 2404
BspTI CTTAAG 1 cut(s) 115
BspTNI GGTCTC 2 cut(s) 860, 2556
BsrDI GCAATG 5 cut(s) 365, 657, 2137, 2224, 3082
BsrGI TGTACA 2 cut(s) 972, 1281
BsrI ACTGG 5 cut(s) 278, 2176, 2238, 2568, 3142
BssECI CCNNGG 5 cut(s) 1208, 2012, 2738, 2897, 3097
BssSI CACGAG 1 cut(s) 3355
BssT1I CCWWGG 3 cut(s) 1208, 2012, 2738
Bst2BI CACGAG 1 cut(s) 3355
Bst2UI CCWGG 4 cut(s) 2163, 2274, 2296, 2898
Bst4CI ACNGT 9 cut(s) 141, 269, 335, 814, 1124, 1187, 1564, 1651, 2721
Bst6I CTCTTC 2 cut(s) 2587, 2745
BstAFI CTTAAG 1 cut(s) 115
BstAPI GCANNNNNTGC 3 cut(s) 311, 1889, 2105
BstAUI TGTACA 2 cut(s) 972, 1281
BstBAI YACGTR 3 cut(s) 623, 1598, 1895
BstC8I GCNNGC 3 cut(s) 65, 340, 1808
BstDEI CTNAG 8 cut(s) 226, 323, 560, 805, 1071, 2553, 2653, 2929
BstDSI CCRYGG 2 cut(s) 2738, 3097
BstENI CCTNNNNNAGG 1 cut(s) 1575
BstF5I GGATG 6 cut(s) 398, 403, 1840, 1987, 2032, 2944
BstFNI CGCG 1 cut(s) 63
BstMAI GTCTC 4 cut(s) 542, 860, 1911, 2556
BstMWI GCNNNNNNNGC 5 cut(s) 311, 1391, 1889, 2105, 2201
BstNI CCWGG 4 cut(s) 2163, 2274, 2296, 2898
BstNSI RCATGY 5 cut(s) 256, 705, 827, 866, 2337
BstSCI CCNGG 5 cut(s) 742, 2161, 2272, 2294, 2896
BstSFI CTRYAG 2 cut(s) 1881, 2121
BstSLI GKGCMC 3 cut(s) 1387, 1894, 2197
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 3 cut(s) 1269, 2270, 2825
BstV2I GAAGAC 2 cut(s) 1716, 2061
BstX2I RGATCY 2 cut(s) 2630, 2910
BstYI RGATCY 2 cut(s) 2630, 2910
BsuI GTATCC 2 cut(s) 1443, 1733
BsuRI GGCC 4 cut(s) 504, 1624, 2732, 2902
BtgI CCRYGG 2 cut(s) 2738, 3097
BtgZI GCGATG 2 cut(s) 44, 2076
BtrI CACGTC 2 cut(s) 435, 2866
BtsCI GGATG 6 cut(s) 398, 403, 1840, 1987, 2032, 2944
BtsI GCAGTG 1 cut(s) 1878
BtsIMutI CAGTG 5 cut(s) 331, 1446, 1878, 1894, 2013
Cac8I GCNNGC 3 cut(s) 65, 340, 1808
CaiI CAGNNNCTG 2 cut(s) 1154, 2903
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 7 cut(s) 661, 1004, 1301, 1622, 2159, 2560, 2900
CseI GACGC 1 cut(s) 3131
DdeI CTNAG 8 cut(s) 226, 323, 560, 805, 1071, 2553, 2653, 2929
DraI TTTAAA 2 cut(s) 2302, 2452
EaeI YGGCCR 1 cut(s) 2730
Eam1104I CTCTTC 2 cut(s) 2587, 2745
EarI CTCTTC 2 cut(s) 2587, 2745
Eco130I CCWWGG 3 cut(s) 1208, 2012, 2738
Eco31I GGTCTC 2 cut(s) 860, 2556
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 5 cut(s) 661, 1004, 1301, 2159, 2560
Eco57I CTGAAG 3 cut(s) 1422, 1557, 2016
Eco72I CACGTG 1 cut(s) 623
EcoNI CCTNNNNNAGG 1 cut(s) 1575
EcoO109I RGGNCCY 2 cut(s) 1622, 2900
EcoRI GAATTC 1 cut(s) 2966
EcoRII CCWGG 4 cut(s) 2161, 2272, 2294, 2896
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 3 cut(s) 1208, 2012, 2738
EcoT22I ATGCAT 2 cut(s) 2326, 2418
ErhI CCWWGG 3 cut(s) 1208, 2012, 2738
FalI AAGNNNNNCTT 2 cut(s) 1577, 1609
FaqI GGGAC 2 cut(s) 759, 2182
FauI CCCGC 2 cut(s) 56, 331
FauNDI CATATG 4 cut(s) 388, 870, 1009, 1758
FbaI TGATCA 2 cut(s) 199, 2221
FblI GTMKAC 2 cut(s) 1171, 1293
Fnu4HI GCNGC 6 cut(s) 505, 1258, 1686, 2259, 2618, 2814
FokI GGATG 6 cut(s) 405, 410, 1847, 1994, 2039, 2951
Fsp4HI GCNGC 6 cut(s) 505, 1258, 1686, 2259, 2618, 2814
FspBI CTAG 6 cut(s) 105, 294, 1388, 1517, 2013, 3156
GluI GCNGC 6 cut(s) 505, 1258, 1686, 2259, 2618, 2814
GsuI CTGGAG 1 cut(s) 2278
HaeIII GGCC 4 cut(s) 504, 1624, 2732, 2902
HapII CCGG 2 cut(s) 743, 965
HgaI GACGC 1 cut(s) 3131
HincII GTYRAC 3 cut(s) 2578, 2863, 3012
HindII GTYRAC 3 cut(s) 2578, 2863, 3012
HindIII AAGCTT 1 cut(s) 557
HpaI GTTAAC 2 cut(s) 2578, 3012
HpaII CCGG 2 cut(s) 743, 965
HphI GGTGA 4 cut(s) 209, 1130, 2759, 2976
Hpy99I CGWCG 1 cut(s) 2725
HpyAV CCTTC 8 cut(s) 609, 772, 1446, 1571, 1581, 1773, 2997, 3317
HpyCH4III ACNGT 9 cut(s) 141, 269, 335, 814, 1124, 1187, 1564, 1651, 2721
HpyCH4IV ACGT 8 cut(s) 16, 283, 434, 622, 850, 1597, 1894, 2865
HpyF10VI GCNNNNNNNGC 5 cut(s) 311, 1391, 1889, 2105, 2201
HpyF3I CTNAG 8 cut(s) 226, 323, 560, 805, 1071, 2553, 2653, 2929
HpySE526I ACGT 8 cut(s) 16, 283, 434, 622, 850, 1597, 1894, 2865
Ksp22I TGATCA 2 cut(s) 199, 2221
KspAI GTTAAC 2 cut(s) 2578, 3012
LmnI GCTCC 3 cut(s) 170, 1013, 3034
Lsp1109I GCAGC 3 cut(s) 1269, 2270, 2825
MaeI CTAG 6 cut(s) 105, 294, 1388, 1517, 2013, 3156
MaeII ACGT 8 cut(s) 16, 283, 434, 622, 850, 1597, 1894, 2865
MaeIII GTNAC 8 cut(s) 150, 329, 440, 510, 572, 1312, 1504, 1786
MflI RGATCY 2 cut(s) 2630, 2910
MhlI GDGCHC 5 cut(s) 1018, 1387, 1894, 2197, 3031
MlsI TGGCCA 1 cut(s) 2732
MluNI TGGCCA 1 cut(s) 2732
MlyI GAGTC 5 cut(s) 1100, 1902, 2762, 2921, 3198
MmeI TCCRAC 4 cut(s) 125, 1750, 2539, 3130
Mox20I TGGCCA 1 cut(s) 2732
Mph1103I ATGCAT 2 cut(s) 2326, 2418
MroXI GAANNNNTTC 1 cut(s) 2781
MscI TGGCCA 1 cut(s) 2732
MslI CAYNNNNRTG 7 cut(s) 391, 828, 1143, 1239, 1757, 2805, 3381
Msp20I TGGCCA 1 cut(s) 2732
MspCI CTTAAG 1 cut(s) 115
MspI CCGG 2 cut(s) 743, 965
MspR9I CCNGG 5 cut(s) 744, 2163, 2274, 2296, 2898
MvaI CCWGG 4 cut(s) 2163, 2274, 2296, 2898
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 5 cut(s) 311, 1391, 1889, 2105, 2201
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 2738
NdeI CATATG 4 cut(s) 388, 870, 1009, 1758
NlaIV GGNNCC 2 cut(s) 2406, 2912
NmeAIII GCCGAG 1 cut(s) 3217
NmuCI GTSAC 4 cut(s) 150, 329, 440, 1786
NsiI ATGCAT 2 cut(s) 2326, 2418
NspI RCATGY 5 cut(s) 256, 705, 827, 866, 2337
OliI CACNNNNGTG 1 cut(s) 3381
PagI TCATGA 1 cut(s) 79
PciI ACATGT 2 cut(s) 823, 862
PdmI GAANNNNTTC 1 cut(s) 2781
PflMI CCANNNNNTGG 1 cut(s) 2739
PfoI TCCNGGA 1 cut(s) 2161
PkrI GCNGC 6 cut(s) 506, 1259, 1687, 2260, 2619, 2815
PleI GAGTC 5 cut(s) 1100, 1902, 2761, 2921, 3198
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 5 cut(s) 1100, 1902, 2761, 2921, 3198
Ppu21I YACGTR 3 cut(s) 623, 1598, 1895
PscI ACATGT 2 cut(s) 823, 862
PsiI TTATAA 2 cut(s) 51, 2885
Psp6I CCWGG 4 cut(s) 2161, 2272, 2294, 2896
PspCI CACGTG 1 cut(s) 623
PspGI CCWGG 4 cut(s) 2161, 2272, 2294, 2896
PspN4I GGNNCC 2 cut(s) 2406, 2912
PspPI GGNCC 7 cut(s) 661, 1004, 1301, 1622, 2159, 2560, 2900
PsrI GAACNNNNNNTAC 4 cut(s) 1497, 1529, 2694, 2726
PstI CTGCAG 1 cut(s) 1885
PstNI CAGNNNCTG 2 cut(s) 1154, 2903
PsuI RGATCY 2 cut(s) 2630, 2910
RseI CAYNNNNRTG 7 cut(s) 391, 828, 1143, 1239, 1757, 2805, 3381
SatI GCNGC 6 cut(s) 505, 1258, 1686, 2259, 2618, 2814
Sau96I GGNCC 7 cut(s) 661, 1004, 1301, 1622, 2159, 2560, 2900
SchI GAGTC 5 cut(s) 1100, 1902, 2762, 2921, 3198
ScrFI CCNGG 5 cut(s) 744, 2163, 2274, 2296, 2898
SduI GDGCHC 5 cut(s) 1018, 1387, 1894, 2197, 3031
SfcI CTRYAG 2 cut(s) 1881, 2121
SinI GGWCC 5 cut(s) 661, 1004, 1301, 2159, 2560
SmiMI CAYNNNNRTG 7 cut(s) 391, 828, 1143, 1239, 1757, 2805, 3381
SmlI CTYRAG 3 cut(s) 115, 2049, 2209
SmoI CTYRAG 3 cut(s) 115, 2049, 2209
SsiI CCGC 6 cut(s) 63, 112, 338, 505, 1685, 2617
SspI AATATT 2 cut(s) 1407, 3052
SspMI CTAG 6 cut(s) 105, 294, 1388, 1517, 2013, 3156
StyD4I CCNGG 5 cut(s) 742, 2161, 2272, 2294, 2896
StyI CCWWGG 3 cut(s) 1208, 2012, 2738
TaaI ACNGT 9 cut(s) 141, 269, 335, 814, 1124, 1187, 1564, 1651, 2721
TaiI ACGT 8 cut(s) 19, 286, 437, 625, 853, 1600, 1897, 2868
TaqI TCGA 6 cut(s) 496, 837, 934, 1245, 1327, 2925
TatI WGTACW 4 cut(s) 972, 1281, 2230, 2916
TauI GCSGC 3 cut(s) 507, 1688, 2620
TscAI CASTG 5 cut(s) 338, 1446, 1885, 1894, 2013
TseFI GTSAC 4 cut(s) 150, 329, 440, 1786
TseI GCWGC 3 cut(s) 1257, 2258, 2813
Tsp45I GTSAC 4 cut(s) 150, 329, 440, 1786
TspGWI ACGGA 3 cut(s) 1188, 1941, 2923
TspRI CASTG 5 cut(s) 338, 1446, 1885, 1894, 2013
Van91I CCANNNNNTGG 1 cut(s) 2739
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 3 cut(s) 1383, 1890, 2193
VpaK11BI GGWCC 5 cut(s) 661, 1004, 1301, 2159, 2560
XagI CCTNNNNNAGG 1 cut(s) 1575
XapI RAATTY 9 cut(s) 839, 1357, 1412, 1455, 1838, 2304, 2966, 2974, 3361
XceI RCATGY 5 cut(s) 256, 705, 827, 866, 2337
XmaJI CCTAGG 1 cut(s) 2012
XmiI GTMKAC 2 cut(s) 1171, 1293
XmnI GAANNNNTTC 1 cut(s) 2781
XspI CTAG 6 cut(s) 105, 294, 1388, 1517, 2013, 3156
Zsp2I ATGCAT 2 cut(s) 2326, 2418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.