Rorug06G0318600

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000080
Physical Location & Seq
Forward (+)
47033786 .. 47038531
4746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0318600.1

Sequence Viewer

Length: 3258 bp
ATGGTGAGCAGCAAGAATTCACCTTATGATTGTGGCCAGTTGATAGTTGACATCACCACTACCACTTTTACCAGAGCGAAGAAGAGGTGGCGCATGGCTTATGCAGCCTTGTATGCTGTAAGGGTGATGCTATCACTTCCCAAAGAGCTTATGTACAAGAGGAGCAACTACAAGGACTACTCTGAGATCTCCGAGTATGGATCTTTCGGTGGTTTCCAAGGTCTCAAAGAAAGCCAGCGGGAGAAGGGAGATTCGAACTCTGGTCTCGAGCCTGAGGCTAAAAACTCTTCACCACTGGAGCTACAATCCCCTGCGCATGATGAGTCTCGTTCTTACACTTCCCTTGAAGTGAAACCGAGCACTTCCCCTGATGGAACTGATCACTATAGTTCTGTCCCCAACAACGTTGTCCATGCAGAGCTCGAAAAAATGGTGAAGGAAAAAGACTTGGCTGCTCTTAAGAAGTTTGGAGGAGTTACTGGCATAACCACTTCTCTTGAAACAAATCCTGAGTATGGGCTACCTGGCAACGACGCAGAAATCAACAGACGCCGTGAACTATTTGGCTCAAATACATACCCAAAGCCGCCTTCAAAAGGGCTCCTCTATTTTGTGCTGGACGCTCTGAAAGACATGACGATTCTGATTCTATGCGTCTGTGCAGCACTTGCTCTTGCATTTGGAATCAAAGAGTATGGAGTAGAGGAAGGCTGGTATGAAGGTGGAAGTATCTTTGTAGCTGTAATCATTGTCATTGTTGTCTCAGCTCTCAGTAACTTCAGACAGGAAATCCAATTCGACAAACTGTCTAAAATAAGCAGCAACATCAACGTTGAGGTATTTAGAGACAGACAAAGACGAGAAATCTCCATCTTTGATGTTGTGGTTGGAGATGTCGTCTTCCTTAACATCGGAGATCAAATTCCTGCAGACGGGGTGTTCTTAGATGGCTATTCATTTCAAGTGGACGAGTCCAGCATGACCGGGGAGAGCGACTATGTAGAAGTTGATTCCAGAAAGAATCCCTTCCTCTTGTCTGGTGCAAAGGTGGCTGATGGTTATGGTAGAATGCTTGTCACATCAGTTGGGATGAACACTGCTTGGGGCGAAATGATGAGCTCAATTTCGCAGGAATCAAATGTAAGAACTCCATTACAAGCTCGGCTTGACAAACTTACCTCTTCTATTGGAAAGGTAGGCCTTGCAGTTGCTTTCTTAGTTCTTCTAGTCTTGTTGATTCGTTATTTCACTGGCAACACAAAAGATAAGAATGGCAATAAGGAGTATATTGGTAGCAAAACAAAAACAGATGATGTGTTGAATGCAGTAGTACGGATTGTATCTGCTGCAGTCACTATTGTGGTGGTGGCAATCCCTGAAGGCTTGCCACTGGCTGTCACACTAACACTTGCTTACTCTATGAAAAGAATGATGAAAGATCAGGCAATGGTTAGAAAACTATCGGCTTGTGAGACAATGGGGTCAGCAACCGTTATCTGTACTGACAAAACAGGAACTCTGACACTAAACCAAATGAAAGTAACTAAGCTTTGGGTAGGCCAAGAATCTATTGCAGAAGATAATTCATCCATCACACCAAATGTTCGTGAGTTATTCTATCAAGGAGTTGGTTTGAACACAACTGGGAGTGTTTTCATGCCTCGTACAGGATCCGAACCCGAAATCTCTGGCAGCCCAACAGAAAAAGCCATACTCTATTGGGTTGTTTTAGATTTGGGTATGGACATGCAAGAACTGAAGCCCAGGTATGACATTCTTCATGTTGAAACATTTAATTCAGACAAGAAACGAAGTGGGGTTCTTATAAAGAAAAAAGATGATAACACCGTCCATGTGCACTGGAAAGGAGCTGCTGAGATGATAGTTGCAACATGCTCAAGTTATTATGATACCAATGGAACTGTAAAGCCCCTGGATGTAGATACAAGGTCTAAAATTGAGAAAATAATCCAAGGCATGGCAGCTAGTAGCCTCCGATGCATTGCTTTCGCTCATGCCCAAATTTCAGAGGAAGAAGTTGAATACAGTGATGATGACAAGACGCACCAAAAGCTCAAAGAAGATGGGCTGATCTTGCTAGGGATAGTCGGCCTCAAAGATCCATGTCGACCAGGGGTCGTCCATGCTGTGAGAGTTTGCAAGGATGCAGGTGTGGACATCAAAATGATAACTGGAGACAATATTTTCACAGCAAAAGCTATAGCTACAGAATGTGGAATACTAAAGAAAGAGAACGAGGTAAAGGATGGACAAGTTGTAGAAGGAGTCGAATTTCGAAACTACACTGACGCAGAGAGAATGCAGAAGGTTGATAACATCCGTGTGATGGCAAGGTCCTCCCCATTTGACAAGCTTCTAATGGTACAGTGTTTGAAGGAGAAAGGTCATGTGGTTGCAGTCACTGGTGATGGTACGAATGATGCACCTGCATTGAAAGAAGCTGATATAGGACTTTCCATGGGCATCCAAGGAACTGAGGTGGCAAAAGAAAGCTCGGATATCATCATCTTGGATGACAACTTCAATTCTGTGGCCACAGTTTTGAGATGGGGGAGATGTGTTTATAACAATATACAGAAATTCATCCAATTTCAACTAACTGTTAATGTTGCAGCTCTAGTAATCAACTTTATTGCAGCAGTTTCAGCTGGTGAAGTTCCCCTAACAGCAGTTCAATTACTTTGGGTGAATCTCATCATGGACACGCTAGGTGCTCTGGCTCTTGCTACGGAGCGGCCAACTAATGAGCTAATGGAGAGGCAGCCTGTGGGTCGGACCGCTCCACTCATAACCAATATTATGTGGAGGAATCTTTTAGCTCAAGCTCTATATCAAATAGCTGTTCTCTTGATCTTGCAGTTCCAGGGTGAGTCTATATTCAATGTCACTGCTGCGGTGAATGATACTCTGATTTTCAATACCTTTGTATTATGCCAAGTTTTCAACGAGTTCAATTCAAGAAGCATGGAGAAGAAGAATGTATTCAAAGGTATTCTCAGGAATAGGCTGTTTATTGGGATTGTGGGGATAACTATTGTTCTTCAGGTTGTGATGGTGGAGTTTCTGAAGAAATTTGCAGATACAGAAAAGTTGAATGTTCAGCAATGGGTAGTTTGTATCGTGATTGCAGCTGTATCATGGCCAATTGGTTGGGTTGTGAAGTTCATACCTGTTTCAGAGGACCCATACTTCAGAATTATCAAAAGGTCACTCCTCAGATTAAAAAACATCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1085

Amino Acids

119.96

Weight (kDa)

6.02

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 157 - 223 6.7e-12 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 277 - 375 2.4e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 555 - 711 4.1e-31 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 680 - 822 4.7e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 893 - 1063 3.2e-46 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1826, 2586
AarI CACCTGC 2 cut(s) 2159, 2455
AasI GACNNNNNNGTC 1 cut(s) 1480
Acc16I TGCGCA 1 cut(s) 315
Acc36I ACCTGC 2 cut(s) 2159, 2455
AccB7I CCANNNNNTGG 1 cut(s) 1978
AccBSI CCGCTC 2 cut(s) 2755, 2801
AccI GTMKAC 1 cut(s) 2128
AciI CCGC 5 cut(s) 238, 587, 2755, 2799, 2915
AclI AACGTT 2 cut(s) 405, 831
AclWI GGATC 4 cut(s) 208, 1665, 1678, 2114
AcoI YGGCCR 4 cut(s) 34, 2553, 2756, 3161
AcsI RAATTY 6 cut(s) 16, 921, 2022, 2291, 2600, 3092
AcuI CTGAAG 6 cut(s) 763, 1398, 1778, 3047, 3107, 3196
AcyI GRCGYC 1 cut(s) 550
AfaI GTAC 6 cut(s) 155, 1332, 1501, 1666, 2385, 2434
AfiI CCNNNNNNNGG 6 cut(s) 515, 596, 932, 1667, 1978, 2347
AflII CTTAAG 1 cut(s) 458
AhdI GACNNNNNGTC 2 cut(s) 805, 2135
AjnI CCWGG 5 cut(s) 523, 1763, 1932, 2131, 2883
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AleI CACNNNNGTG 1 cut(s) 1358
Alw21I GWGCWC 5 cut(s) 362, 423, 1121, 1860, 2737
Alw26I GTCTC 7 cut(s) 227, 269, 330, 766, 840, 1466, 2190
Alw44I GTGCAC 1 cut(s) 1856
AlwI GGATC 4 cut(s) 208, 1665, 1678, 2114
AlwNI CAGNNNCTG 1 cut(s) 2423
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 7 cut(s) 34, 1198, 1558, 2110, 2553, 2756, 3161
ApaLI GTGCAC 1 cut(s) 1856
ApoI RAATTY 6 cut(s) 16, 921, 2022, 2291, 2600, 3092
Asp700I GAANNNNTTC 2 cut(s) 1025, 3002
AspLEI GCGC 2 cut(s) 93, 316
AspS9I GGNCC 3 cut(s) 2355, 2796, 3202
AsuC2I CCSGG 1 cut(s) 985
AsuII TTCGAA 2 cut(s) 254, 2296
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 3 cut(s) 2355, 2796, 3202
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 1860
BalI TGGCCA 3 cut(s) 36, 2555, 3163
BamHI GGATCC 1 cut(s) 1670
BanII GRGCYC 3 cut(s) 423, 603, 1121
BbsI GAAGAC 1 cut(s) 892
Bbv12I GWGCWC 5 cut(s) 362, 423, 1121, 1860, 2737
BceAI ACGGC 1 cut(s) 537
BcgI CGANNNNNNTGC 2 cut(s) 1990, 2024
BciT130I CCWGG 5 cut(s) 525, 1765, 1934, 2133, 2885
BclI TGATCA 1 cut(s) 379
BcnI CCSGG 1 cut(s) 985
BcoDI GTCTC 7 cut(s) 227, 269, 330, 766, 840, 1466, 2190
BfaI CTAG 5 cut(s) 1226, 1986, 2099, 2639, 2729
BfmI CTRYAG 5 cut(s) 385, 927, 1347, 2220, 2226
BfrI CTTAAG 1 cut(s) 458
BfuAI ACCTGC 2 cut(s) 2159, 2455
BglII AGATCT 1 cut(s) 186
Bme1390I CCNGG 6 cut(s) 525, 985, 1765, 1934, 2133, 2885
Bme18I GGWCC 3 cut(s) 2355, 2796, 3202
BmeRI GACNNNNNGTC 2 cut(s) 805, 2135
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 2355, 2796, 3202
BmiI GGNNCC 3 cut(s) 602, 1672, 3204
BmrFI CCNGG 6 cut(s) 525, 985, 1765, 1934, 2133, 2885
BmrI ACTGGG 1 cut(s) 1653
BmsI GCATC 5 cut(s) 117, 1988, 2155, 2431, 2493
BmuI ACTGGG 1 cut(s) 1653
BpiI GAAGAC 1 cut(s) 892
BpmI CTGGAG 2 cut(s) 317, 2214
Bpu14I TTCGAA 2 cut(s) 254, 2296
BpuEI CTTGAG 2 cut(s) 1882, 2826
BpuMI CCSGG 1 cut(s) 985
BsaHI GRCGYC 1 cut(s) 550
BsaI GGTCTC 2 cut(s) 227, 269
BsaJI CCNNGG 9 cut(s) 217, 984, 1763, 1932, 1972, 2132, 2478, 2488, 2884
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 6 cut(s) 515, 596, 932, 1667, 1978, 2347
Bse1I ACTGG 9 cut(s) 37, 300, 484, 1255, 1395, 1648, 1865, 2197, 2428
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 3 cut(s) 1452, 2001, 3131
BseBI CCWGG 5 cut(s) 525, 1765, 1934, 2133, 2885
BseDI CCNNGG 9 cut(s) 217, 984, 1763, 1932, 1972, 2132, 2478, 2488, 2884
BseGI GGATG 9 cut(s) 1095, 1586, 1942, 2170, 2272, 2337, 2484, 2539, 2604
BseLI CCNNNNNNNGG 6 cut(s) 515, 596, 932, 1667, 1978, 2347
BseMI GCAATG 3 cut(s) 1452, 2001, 3131
BseMII CTCAG 9 cut(s) 174, 264, 501, 777, 784, 1866, 2487, 3031, 3250
BseNI ACTGG 9 cut(s) 37, 300, 484, 1255, 1395, 1648, 1865, 2197, 2428
BseRI GAGGAG 4 cut(s) 175, 486, 593, 3224
BseSI GKGCMC 1 cut(s) 1860
BsgI GTGCAG 1 cut(s) 681
BshFI GGCC 7 cut(s) 36, 1200, 1560, 2112, 2555, 2758, 3163
BsiHKAI GWGCWC 5 cut(s) 362, 423, 1121, 1860, 2737
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 984
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 6 cut(s) 515, 596, 932, 1667, 1978, 2347
BsmAI GTCTC 7 cut(s) 227, 269, 330, 766, 840, 1466, 2190
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 3 cut(s) 1074, 1327, 2325
BsnI GGCC 7 cut(s) 36, 1200, 1560, 2112, 2555, 2758, 3163
Bso31I GGTCTC 2 cut(s) 227, 269
BsoBI CYCGRG 1 cut(s) 266
Bsp119I TTCGAA 2 cut(s) 254, 2296
Bsp1286I GDGCHC 6 cut(s) 362, 423, 603, 1121, 1860, 2737
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
Bsp19I CCATGG 1 cut(s) 2478
BspACI CCGC 5 cut(s) 238, 587, 2755, 2799, 2915
BspANI GGCC 7 cut(s) 36, 1200, 1560, 2112, 2555, 2758, 3163
BspCNI CTCAG 9 cut(s) 175, 265, 502, 776, 783, 1867, 2488, 3030, 3249
BspLI GGNNCC 3 cut(s) 602, 1672, 3204
BspMAI CTGCAG 2 cut(s) 931, 1351
BspMI ACCTGC 2 cut(s) 2159, 2455
BspPI GGATC 4 cut(s) 208, 1665, 1678, 2114
BspT104I TTCGAA 2 cut(s) 254, 2296
BspTI CTTAAG 1 cut(s) 458
BspTNI GGTCTC 2 cut(s) 227, 269
BsrBI CCGCTC 2 cut(s) 2755, 2801
BsrDI GCAATG 3 cut(s) 1452, 2001, 3131
BsrGI TGTACA 1 cut(s) 153
BsrI ACTGG 9 cut(s) 37, 300, 484, 1255, 1395, 1648, 1865, 2197, 2428
BssECI CCNNGG 9 cut(s) 217, 984, 1763, 1932, 1972, 2132, 2478, 2488, 2884
BssMI GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
BssNI GRCGYC 1 cut(s) 550
BssT1I CCWWGG 4 cut(s) 217, 1972, 2478, 2488
Bst2UI CCWGG 5 cut(s) 525, 1765, 1934, 2133, 2885
Bst4CI ACNGT 8 cut(s) 807, 1492, 1849, 1924, 2048, 2388, 2560, 2623
Bst6I CTCTTC 3 cut(s) 77, 292, 1186
BstACI GRCGYC 1 cut(s) 550
BstAFI CTTAAG 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 668
BstAUI TGTACA 1 cut(s) 153
BstBI TTCGAA 2 cut(s) 254, 2296
BstC8I GCNNGC 2 cut(s) 236, 1385
BstDSI CCRYGG 1 cut(s) 2478
BstENI CCTNNNNNAGG 2 cut(s) 594, 1665
BstF5I GGATG 9 cut(s) 1095, 1586, 1942, 2170, 2272, 2337, 2484, 2539, 2604
BstHHI GCGC 2 cut(s) 93, 316
BstKTI GATC 9 cut(s) 189, 203, 382, 919, 1441, 1673, 2094, 2122, 2874
BstMAI GTCTC 7 cut(s) 227, 269, 330, 766, 840, 1466, 2190
BstMBI GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
BstMWI GCNNNNNNNGC 8 cut(s) 104, 113, 668, 1049, 1998, 2071, 2095, 2666
BstNI CCWGG 5 cut(s) 525, 1765, 1934, 2133, 2885
BstNSI RCATGY 2 cut(s) 1750, 1896
BstSCI CCNGG 6 cut(s) 523, 983, 1763, 1932, 2131, 2883
BstSFI CTRYAG 5 cut(s) 385, 927, 1347, 2220, 2226
BstSLI GKGCMC 1 cut(s) 1860
BstV2I GAAGAC 1 cut(s) 892
BstX2I RGATCY 4 cut(s) 186, 200, 1670, 2119
BstXI CCANNNNNNTGG 1 cut(s) 3171
BstYI RGATCY 4 cut(s) 186, 200, 1670, 2119
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 7 cut(s) 36, 1200, 1560, 2112, 2555, 2758, 3163
BtgI CCRYGG 1 cut(s) 2478
BtsCI GGATG 9 cut(s) 1095, 1586, 1942, 2170, 2272, 2337, 2484, 2539, 2604
BtsI GCAGTG 2 cut(s) 1095, 2907
BveI ACCTGC 2 cut(s) 2159, 2455
Cac8I GCNNGC 2 cut(s) 236, 1385
CaiI CAGNNNCTG 1 cut(s) 2423
CfoI GCGC 2 cut(s) 93, 316
Cfr13I GGNCC 3 cut(s) 2355, 2796, 3202
CpoI CGGWCCG 1 cut(s) 2796
CseI GACGC 6 cut(s) 542, 558, 629, 643, 2071, 2318
Csp6I GTAC 6 cut(s) 154, 1331, 1500, 1665, 2384, 2433
CspCI CAANNNNNGTGG 2 cut(s) 478, 513
CspI CGGWCCG 1 cut(s) 2796
CviQI GTAC 6 cut(s) 154, 1331, 1500, 1665, 2384, 2433
DpnI GATC 9 cut(s) 188, 202, 381, 918, 1440, 1672, 2093, 2121, 2873
DpnII GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
DrdI GACNNNNNNGTC 1 cut(s) 1480
DriI GACNNNNNGTC 2 cut(s) 805, 2135
DseDI GACNNNNNNGTC 1 cut(s) 1480
EaeI YGGCCR 4 cut(s) 34, 2553, 2756, 3161
Eam1104I CTCTTC 3 cut(s) 77, 292, 1186
Eam1105I GACNNNNNGTC 2 cut(s) 805, 2135
EarI CTCTTC 3 cut(s) 77, 292, 1186
Ecl136II GAGCTC 2 cut(s) 421, 1119
Eco130I CCWWGG 4 cut(s) 217, 1972, 2478, 2488
Eco147I AGGCCT 1 cut(s) 1200
Eco24I GRGCYC 3 cut(s) 423, 603, 1121
Eco31I GGTCTC 2 cut(s) 227, 269
Eco32I GATATC 1 cut(s) 2521
Eco47I GGWCC 3 cut(s) 2355, 2796, 3202
Eco53kI GAGCTC 2 cut(s) 421, 1119
Eco57I CTGAAG 6 cut(s) 763, 1398, 1778, 3047, 3107, 3196
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 2 cut(s) 421, 1119
EcoNI CCTNNNNNAGG 2 cut(s) 594, 1665
EcoO109I RGGNCCY 2 cut(s) 2355, 3202
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 5 cut(s) 523, 1763, 1932, 2131, 2883
EcoRV GATATC 1 cut(s) 2521
EcoT14I CCWWGG 4 cut(s) 217, 1972, 2478, 2488
EcoT22I ATGCAT 1 cut(s) 2003
EcoT38I GRGCYC 3 cut(s) 423, 603, 1121
ErhI CCWWGG 4 cut(s) 217, 1972, 2478, 2488
FalI AAGNNNNNCTT 4 cut(s) 1010, 1042, 1149, 1181
FaqI GGGAC 1 cut(s) 380
FauI CCCGC 1 cut(s) 231
FbaI TGATCA 1 cut(s) 379
FblI GTMKAC 1 cut(s) 2128
FokI GGATG 9 cut(s) 1102, 1573, 1949, 2177, 2279, 2324, 2471, 2546, 2591
FriOI GRGCYC 3 cut(s) 423, 603, 1121
FspBI CTAG 5 cut(s) 1226, 1986, 2099, 2639, 2729
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 2 cut(s) 92, 315
GsuI CTGGAG 2 cut(s) 317, 2214
HaeIII GGCC 7 cut(s) 36, 1200, 1560, 2112, 2555, 2758, 3163
HapII CCGG 1 cut(s) 984
HgaI GACGC 6 cut(s) 542, 558, 629, 643, 2071, 2318
HhaI GCGC 2 cut(s) 93, 316
Hin1I GRCGYC 1 cut(s) 550
Hin6I GCGC 2 cut(s) 91, 314
HinP1I GCGC 2 cut(s) 91, 314
HincII GTYRAC 2 cut(s) 49, 2129
HindII GTYRAC 2 cut(s) 49, 2129
HindIII AAGCTT 2 cut(s) 1547, 2372
HpaII CCGG 1 cut(s) 984
Hpy166II GTNNAC 6 cut(s) 49, 557, 967, 1858, 2129, 2176
Hpy188III TCNNGA 9 cut(s) 266, 497, 509, 1014, 1607, 2869, 2979, 3019, 3142
Hpy8I GTNNAC 6 cut(s) 49, 557, 967, 1858, 2129, 2176
Hpy99I CGWCG 1 cut(s) 536
HpyCH4III ACNGT 8 cut(s) 807, 1492, 1849, 1924, 2048, 2388, 2560, 2623
HpyCH4IV ACGT 2 cut(s) 405, 831
HpyF10VI GCNNNNNNNGC 8 cut(s) 104, 113, 668, 1049, 1998, 2071, 2095, 2666
HpySE526I ACGT 2 cut(s) 405, 831
Hsp92I GRCGYC 1 cut(s) 550
HspAI GCGC 2 cut(s) 91, 314
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
LmnI GCTCC 6 cut(s) 162, 298, 606, 1868, 2752, 2806
LweI GCATC 5 cut(s) 117, 1988, 2155, 2431, 2493
MaeI CTAG 5 cut(s) 1226, 1986, 2099, 2639, 2729
MaeII ACGT 2 cut(s) 405, 831
MaeIII GTNAC 9 cut(s) 475, 773, 1075, 1351, 1396, 1540, 2419, 2905, 3228
MalI GATC 9 cut(s) 188, 202, 381, 918, 1440, 1672, 2093, 2121, 2873
MbiI CCGCTC 2 cut(s) 2755, 2801
MboI GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
MfeI CAATTG 1 cut(s) 3165
MflI RGATCY 4 cut(s) 186, 200, 1670, 2119
MhlI GDGCHC 6 cut(s) 362, 423, 603, 1121, 1860, 2737
MlsI TGGCCA 3 cut(s) 36, 2555, 3163
MluNI TGGCCA 3 cut(s) 36, 2555, 3163
MlyI GAGTC 4 cut(s) 332, 980, 2295, 2900
MmeI TCCRAC 2 cut(s) 868, 2774
Mox20I TGGCCA 3 cut(s) 36, 2555, 3163
Mph1103I ATGCAT 1 cut(s) 2003
MroXI GAANNNNTTC 2 cut(s) 1025, 3002
MscI TGGCCA 3 cut(s) 36, 2555, 3163
MseI TTAA 5 cut(s) 459, 906, 1794, 2625, 3242
MslI CAYNNNNRTG 3 cut(s) 1358, 2183, 2342
Msp20I TGGCCA 3 cut(s) 36, 2555, 3163
MspA1I CMGCKG 3 cut(s) 238, 2669, 3152
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 1 cut(s) 984
MspR9I CCNGG 6 cut(s) 525, 985, 1765, 1934, 2133, 2885
MunI CAATTG 1 cut(s) 3165
Mva1269I GAATGC 3 cut(s) 1074, 1327, 2325
MvaI CCWGG 5 cut(s) 525, 1765, 1934, 2133, 2885
MwoI GCNNNNNNNGC 8 cut(s) 104, 113, 668, 1049, 1998, 2071, 2095, 2666
NciI CCSGG 1 cut(s) 985
NcoI CCATGG 1 cut(s) 2478
NdeII GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
NlaIV GGNNCC 3 cut(s) 602, 1672, 3204
NmeAIII GCCGAG 1 cut(s) 1141
NmuCI GTSAC 6 cut(s) 1075, 1351, 1396, 2419, 2905, 3228
NsbI TGCGCA 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 2003
NspI RCATGY 2 cut(s) 1750, 1896
NspV TTCGAA 2 cut(s) 254, 2296
OliI CACNNNNGTG 1 cut(s) 1358
PaeR7I CTCGAG 1 cut(s) 266
PaqCI CACCTGC 2 cut(s) 2159, 2455
PceI AGGCCT 1 cut(s) 1200
PctI GAATGC 3 cut(s) 1074, 1327, 2325
PdmI GAANNNNTTC 2 cut(s) 1025, 3002
PflMI CCANNNNNTGG 1 cut(s) 1978
PleI GAGTC 4 cut(s) 331, 979, 2294, 2899
PpsI GAGTC 4 cut(s) 331, 979, 2294, 2899
PpuMI RGGWCCY 2 cut(s) 2355, 3202
PsiI TTATAA 2 cut(s) 1826, 2586
Psp124BI GAGCTC 2 cut(s) 423, 1121
Psp1406I AACGTT 2 cut(s) 405, 831
Psp5II RGGWCCY 2 cut(s) 2355, 3202
Psp6I CCWGG 5 cut(s) 523, 1763, 1932, 2131, 2883
PspGI CCWGG 5 cut(s) 523, 1763, 1932, 2131, 2883
PspN4I GGNNCC 3 cut(s) 602, 1672, 3204
PspPI GGNCC 3 cut(s) 2355, 2796, 3202
PspPPI RGGWCCY 2 cut(s) 2355, 3202
PstI CTGCAG 2 cut(s) 931, 1351
PstNI CAGNNNCTG 1 cut(s) 2423
PsuI RGATCY 4 cut(s) 186, 200, 1670, 2119
PvuII CAGCTG 2 cut(s) 2669, 3152
RsaI GTAC 6 cut(s) 155, 1332, 1501, 1666, 2385, 2434
RsaNI GTAC 6 cut(s) 154, 1331, 1500, 1665, 2384, 2433
RseI CAYNNNNRTG 3 cut(s) 1358, 2183, 2342
Rsr2I CGGWCCG 1 cut(s) 2796
RsrII CGGWCCG 1 cut(s) 2796
SacI GAGCTC 2 cut(s) 423, 1121
SalI GTCGAC 1 cut(s) 2127
SaqAI TTAA 5 cut(s) 459, 906, 1794, 2625, 3242
Sau3AI GATC 9 cut(s) 186, 200, 379, 916, 1438, 1670, 2091, 2119, 2871
Sau96I GGNCC 3 cut(s) 2355, 2796, 3202
SchI GAGTC 4 cut(s) 332, 980, 2295, 2900
ScrFI CCNGG 6 cut(s) 525, 985, 1765, 1934, 2133, 2885
SduI GDGCHC 6 cut(s) 362, 423, 603, 1121, 1860, 2737
SfaNI GCATC 5 cut(s) 117, 1988, 2155, 2431, 2493
SfcI CTRYAG 5 cut(s) 385, 927, 1347, 2220, 2226
Sfr274I CTCGAG 1 cut(s) 266
SfuI TTCGAA 2 cut(s) 254, 2296
SinI GGWCC 3 cut(s) 2355, 2796, 3202
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 3 cut(s) 1358, 2183, 2342
SmlI CTYRAG 4 cut(s) 266, 458, 1897, 2841
SmoI CTYRAG 4 cut(s) 266, 458, 1897, 2841
SseBI AGGCCT 1 cut(s) 1200
SsiI CCGC 5 cut(s) 238, 587, 2755, 2799, 2915
SspI AATATT 2 cut(s) 2203, 2818
SspMI CTAG 5 cut(s) 1226, 1986, 2099, 2639, 2729
SstI GAGCTC 2 cut(s) 423, 1121
StuI AGGCCT 1 cut(s) 1200
StyD4I CCNGG 6 cut(s) 523, 983, 1763, 1932, 2131, 2883
StyI CCWWGG 4 cut(s) 217, 1972, 2478, 2488
TaaI ACNGT 8 cut(s) 807, 1492, 1849, 1924, 2048, 2388, 2560, 2623
TaiI ACGT 2 cut(s) 408, 834
TaqI TCGA 7 cut(s) 254, 267, 423, 798, 2128, 2289, 2296
TatI WGTACW 2 cut(s) 153, 1499
TauI GCSGC 2 cut(s) 589, 2758
Tru1I TTAA 5 cut(s) 459, 906, 1794, 2625, 3242
Tru9I TTAA 5 cut(s) 459, 906, 1794, 2625, 3242
TseFI GTSAC 6 cut(s) 1075, 1351, 1396, 2419, 2905, 3228
Tsp45I GTSAC 6 cut(s) 1075, 1351, 1396, 2419, 2905, 3228
TspGWI ACGGA 3 cut(s) 1348, 2330, 2765
Van91I CCANNNNNTGG 1 cut(s) 1978
Vha464I CTTAAG 1 cut(s) 458
VneI GTGCAC 1 cut(s) 1856
VpaK11BI GGWCC 3 cut(s) 2355, 2796, 3202
XagI CCTNNNNNAGG 2 cut(s) 594, 1665
XapI RAATTY 6 cut(s) 16, 921, 2022, 2291, 2600, 3092
XceI RCATGY 2 cut(s) 1750, 1896
XhoI CTCGAG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 2128
XmnI GAANNNNTTC 2 cut(s) 1025, 3002
XspI CTAG 5 cut(s) 1226, 1986, 2099, 2639, 2729
Zsp2I ATGCAT 1 cut(s) 2003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.