Rw3G028480

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000080
Physical Location & Seq
Reverse (-)
42478829 .. 42482622
3794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G028480.1

Sequence Viewer

Length: 3390 bp
ATGTGTTGCTTGAATGGGAAAATTGTAATTTCTCCAGTTCAATCTCCTCCAGAAATGGCCGAGCTATTTTCTGCAGAAACTCCGAGAGGTGCCCATTTTAGGCAGAATATCTATACTTATAATCATGTATTTTCATTCACATCAATGGGCGTGCATGTCGACCAAAATCTAGCGACTGGTGGTGGCACATTCACTTTTCGTGCTCAAGGATCCATATATCATAAGATAGGAACTCTTTTACCAAATGAGGATAACAGACCAAGATTTCTACAACTGTATATATATGATACAGACCATGAACTACAAAATCGTATGTTAGAGAATGAATCATTACATAGAGATGTGGTTGAAACTATACAAGAAATCTTGAATCGGCATAATCCGTTTGTGCAGACACTTCATCACCTAGCACAACGCGAAGATTTGGAAACTTGCAGACTCATCATTAGAGAGCAACCTACAAACCAACGTCAGTATAGTTTGCCTACAGAGTCTCAGGTTGCTGCAGTAATAGTGGGTGCAGATGATGCAGAAAATCTGAGAGGTCACTGTGCGGGCTACTATGATCCTTTACAATATCCATTGCTTTTACCTTATGGCACATATGAATGGGATGTGAATAGTCGGAATAATAATGGTACAAAATTGACGTGTCGTGACTATTACGCATATATTTTGCAGATTCGTCCTGATGATGAATCAATTTTACTTCGAGGAGGCCGCCTGTTACAACAATATGTCGTCGATAATTATGTGAAAATTGAGACACAAAAGCTTAGATGGATTCGCAGTAATCAAGATACCCTGCGACGTGAACTTTATAATGGACTCCAAGACTCGCTAAATGCCGGTGAAAACAATGCATATGCAATGGCTTTGGTCCAAAAATACGGGAAGCCAGAAATTTTCCTCACAATGACATGTAACCCGAATTGGGAAGAAATAAAGGCAGAGTTATTACCGGGACAAGTGGCACAAGATCGCCCTGATTTATGTACCCGGATTTTTCATGCCAAATTTGAAGAGCTGAAGGAAGATGTTATCAAAAAAGGGGTCTTAGGTACTGTTGTTGCACATGTTTATGTCATTGAATTTCAAAAACGTGGTCTCCCACATGTTCATATGCTTCTCATGTTAAATGACGATGATAAGTTGAATAACCCGGATGATTATGACAAAATTGTTCGAGCTGAAATACCAAGTCAAGATGAAGAACCGGAGTTGTACAATGCCGTATTCAGGCACATGATACATGGTCCATGTGGAGCACATAAGCGTAATGCCCCATGTATGAAGCGTGGGAGTTGTAAACGAGGTTATCCTAAGCCCTTTTCAGCCAACACATTTCAAGGGAATGACTCTTATCCCATTTACCTAGATAGCAGTTGGGTTATTCCGTACAACCCTTGGCTACTCACAAAATATGATTGTCATATCAATGTCGAAGTCTGTTGCAGCATTAAGAGTGTTAAGTATTTGTACAAATATGTCTACAAATGTCCAGATAGAGTAACATTTGAAGTTCGACCCGAAGCTAATGAAGATGAAATAAGGAATTTTGTTGATGCAAGATGGGTTTGTGCACCAGAGGCTTTGTGGAGAATATTCAAATTTGTAATGAATCGTATGTATCCTTCAGTGGAACGATTGCAAATTCATCTTCCAAATAGACAGAGTGTTTTCTTTAATGCCAATGAAAGGGTAACAGATGTTCTCGCAAATGATAGAAACTCAATGACGATGCTCACAGAGTTTTTTACTAAGAACATGTATTATGAAGGTGCGTGCCAATACTTATATCGAGAGTTTCCTGAAAGATTCAGATGGATTGGCAAACATAAGACATGGGAGGAAAGACAGACTAACCAAAAAGTAATTGGTAGAATATATACGGTATCTCCTTCAGAAGGAGAAAAGTTCAACTTACGTGTTCTTTTGAACCATGTTAGAGGGCCTACATCATACGAGGATTTGAGGACAGTAGGAGGTCTGTTAGAAGACGATGATAGTATCCGACAATGTTTACATGAGGCTTCAAGCATACATATGCCTTCATCTTTAAGGAGATTGTTTGTCACTATCTTGGTTTATTGCGAGCCTCATGGGGTACGAAGTTTATGGGATGAATTTTACCAATTTATGATAGAAGATTATGCTTCTTCAAGCACTACAGTCAGTGCACGTATCACAAACAGACTCTTACGAGACTTAAACGGATTGCTGGTGCAACATAGTAAATCTGTTTGTGATTATGATTTGCCTGAAATGGATGAAGATTATGGTGAAGATTCGTCAATGCCTAGGCTAATACAGGATGAAGTTTCAATTATCATTCCTCAAGAAGACTGCGATGCAGTTCATCTCTTGAATGAGGATCAGTCATTTGCATACAATGCAATAATATCAGCTATAGAGTGCAATGATAATGCCATATTTTTCGTTGATGGTCCAGGAGGGACTGGAAAAACATACTTATATCGTGCACTTTTAGCAACCTTGAGAAGCAATGATCATATTGTACTGGCAACAACAACTTCTGGAATTGCAGCAACAATAATGCCTGGTGGGAGAACGGCACACTCCAGGTTTAAAATTCCACTCAATCTTGATGCATCTTCTACATGCTCTATTAGTAAACAATCTAATTTAGCAGAGTTGATACGAAGATCATCAGTGATTATTTGTGATGAGGCACCAATGATGCATAGATTTGCATTTGAAGCTCTTGATCGGACTTTTAAAGACATAATAGGTGTTGATTTGCCATTTGGGGGCAAAGTGATTATTTTTGGGGGAGATTTCCGACAAGTTCTGCCCGTTGTCCATAAGGGTACAAGGTCTCAGATGGTCCAAGCCAGTCTGGTTAACTCTTCATTTTGGAAAGATGTGAGGATTCTTCATTTGCGGCAAAATATGAGATCTATCAATGATCCAGAGTTCTCAGAGTTTTTACTTAATGTTGGTAATGGAGGACAACCATCTGTGGTTGAAGACATGATACGATTACTGTCGTGTATGGCCATACCATGGGAGGGTGAAGAGTCAATCAACCGATTAGTTAATGAAGTTTTTCCTAATTCTGAATCTCATGTAAATGATGCAGCGTACATGGTTGAAAGGGCAATAATCACTCCAAAGAATGATGATGTTAACGTGCTTAATGAGATGATTATAAAGCAATTGCCAGGGCCTGAACGAATCATGTACTCTTTCGACTCAGTTGAGGATGACACTAGAAATATGTACCAACAAAAATTATTAAATTCAATTTCACCTCCTGGTATGCCCCCACATCAGTTAACTATAAAAAAAGGTGCTCCCATTATGCTTTTGAGAAATATTGACCCTACGATGGGATTGTGCAATGGTACACGATTGACTTGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1129

Amino Acids

129.61

Weight (kDa)

5.81

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 221 - 286 1.5e-10 Helitron helicase-like domain at N-terminus
Helitron_like_N PF14214 290 - 377 1.8e-36 Helitron helicase-like domain at N-terminus
PIF1 PF05970 789 - 993 2.9e-73 PIF1-like helicase
AAA_30 PF13604 789 - 951 5.8e-06 AAA domain
Pif1_2B_dom PF21530 1086 - 1127 1.2e-18 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 120, 822, 3173
AccB1I GGYRCC 2 cut(s) 89, 2692
AccB7I CCANNNNNTGG 1 cut(s) 3027
AccI GTMKAC 2 cut(s) 159, 1491
AccII CGCG 1 cut(s) 417
AciI CCGC 3 cut(s) 554, 721, 2905
AclWI GGATC 5 cut(s) 204, 217, 560, 2382, 2924
AcoI YGGCCR 2 cut(s) 57, 3018
AcsI RAATTY 9 cut(s) 903, 1016, 1091, 1555, 1610, 1653, 2126, 2592, 3262
AcuI CTGAAG 3 cut(s) 1049, 1620, 1887
AflIII ACRYGT 6 cut(s) 650, 920, 1075, 1114, 1767, 1927
AjiI CACGTC 2 cut(s) 651, 812
AjnI CCWGG 5 cut(s) 2449, 2560, 2582, 3184, 3277
AluBI AGCT 7 cut(s) 64, 775, 1027, 1190, 1535, 2408, 2723
AluI AGCT 7 cut(s) 64, 775, 1027, 1190, 1535, 2408, 2723
Alw21I GWGCWC 6 cut(s) 205, 1270, 1585, 2182, 2485, 3319
Alw26I GTCTC 5 cut(s) 498, 758, 1112, 2199, 2844
Alw44I GTGCAC 3 cut(s) 1581, 2178, 2481
AlwI GGATC 5 cut(s) 204, 217, 560, 2382, 2924
AlwNI CAGNNNCTG 1 cut(s) 3191
AoxI GGCC 5 cut(s) 57, 718, 1952, 3018, 3188
ApaLI GTGCAC 3 cut(s) 1581, 2178, 2481
ApeKI GCWGC 4 cut(s) 503, 1455, 2546, 3101
ApoI RAATTY 9 cut(s) 903, 1016, 1091, 1555, 1610, 1653, 2126, 2592, 3262
ArsI GACNNNNNNTTYG 4 cut(s) 1038, 1070, 1090, 1122
Asp700I GAANNNNTTC 1 cut(s) 3069
AspA2I CCTAGG 1 cut(s) 2300
AspS9I GGNCC 6 cut(s) 880, 1256, 1952, 2447, 2848, 3188
AsuC2I CCSGG 3 cut(s) 963, 1000, 1163
AsuHPI GGTGA 5 cut(s) 395, 863, 2294, 3047, 3264
AvaII GGWCC 4 cut(s) 880, 1256, 2447, 2848
AvrII CCTAGG 1 cut(s) 2300
BaeGI GKGCMC 4 cut(s) 94, 1585, 2182, 2485
BaeI ACNNNNGTAYC 2 cut(s) 1053, 1086
BalI TGGCCA 1 cut(s) 3020
BamHI GGATCC 1 cut(s) 209
BanI GGYRCC 2 cut(s) 89, 2692
BarI GAAGNNNNNNTAC 4 cut(s) 1618, 1650, 2982, 3014
BbsI GAAGAC 3 cut(s) 2004, 2349, 2997
Bbv12I GWGCWC 6 cut(s) 205, 1270, 1585, 2182, 2485, 3319
BbvI GCAGC 4 cut(s) 490, 1467, 2558, 3113
BccI CCATC 7 cut(s) 774, 1566, 1818, 2438, 2839, 2986, 3346
BceAI ACGGC 3 cut(s) 1217, 2589, 3369
BcgI CGANNNNNNTGC 1 cut(s) 3363
BciT130I CCWGG 5 cut(s) 2451, 2562, 2584, 3186, 3279
BciVI GTATCC 2 cut(s) 1641, 2021
BclI TGATCA 1 cut(s) 2509
BcnI CCSGG 3 cut(s) 963, 1000, 1163
BcoDI GTCTC 5 cut(s) 498, 758, 1112, 2199, 2844
BfaI CTAG 5 cut(s) 170, 407, 1376, 2301, 3234
BfmI CTRYAG 5 cut(s) 72, 486, 504, 2169, 2409
BfuI GTATCC 2 cut(s) 1641, 2021
BglII AGATCT 1 cut(s) 2918
BisI GCNGC 6 cut(s) 504, 721, 1456, 2547, 2906, 3102
BlnI CCTAGG 1 cut(s) 2300
BlsI GCNGC 6 cut(s) 505, 722, 1457, 2548, 2907, 3103
Bme1390I CCNGG 8 cut(s) 963, 1000, 1163, 2451, 2562, 2584, 3186, 3279
Bme18I GGWCC 4 cut(s) 880, 1256, 2447, 2848
BmgBI CACGTC 2 cut(s) 651, 812
BmgT120I GGNCC 6 cut(s) 880, 1256, 1952, 2447, 2848, 3188
BmiI GGNNCC 3 cut(s) 91, 211, 2694
BmrFI CCNGG 8 cut(s) 963, 1000, 1163, 2451, 2562, 2584, 3186, 3279
BmsI GCATC 8 cut(s) 517, 1555, 1731, 2341, 2599, 2621, 2691, 3088
BpiI GAAGAC 3 cut(s) 2004, 2349, 2997
BpmI CTGGAG 3 cut(s) 18, 33, 2566
Bpu10I CCTNAGC 1 cut(s) 1323
BpuEI CTTGAG 3 cut(s) 189, 2322, 2518
BpuMI CCSGG 3 cut(s) 963, 1000, 1163
BsaAI YACGTR 2 cut(s) 1928, 2183
BsaI GGTCTC 2 cut(s) 1112, 2844
BsaJI CCNNGG 4 cut(s) 1406, 2300, 3026, 3185
BsaWI WCCGGW 1 cut(s) 1216
BsaXI ACNNNNNCTCC 4 cut(s) 1092, 1122, 1882, 1912
Bse118I RCCGGY 1 cut(s) 848
Bse1I ACTGG 5 cut(s) 35, 181, 2464, 2526, 2856
Bse3DI GCAATG 5 cut(s) 581, 876, 2425, 2512, 3370
BseBI CCWGG 5 cut(s) 2451, 2562, 2584, 3186, 3279
BseDI CCNNGG 4 cut(s) 1406, 2300, 3026, 3185
BseGI GGATG 6 cut(s) 619, 1171, 2128, 2275, 2320, 3232
BseMI GCAATG 5 cut(s) 581, 876, 2425, 2512, 3370
BseMII CTCAG 5 cut(s) 509, 530, 2855, 2955, 3231
BseNI ACTGG 5 cut(s) 35, 181, 2464, 2526, 2856
BseRI GAGGAG 2 cut(s) 36, 729
BseSI GKGCMC 4 cut(s) 94, 1585, 2182, 2485
BseXI GCAGC 4 cut(s) 490, 1467, 2558, 3113
BsgI GTGCAG 2 cut(s) 410, 540
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 5 cut(s) 59, 720, 1954, 3020, 3190
BshNI GGYRCC 2 cut(s) 89, 2692
BsiHKAI GWGCWC 6 cut(s) 205, 1270, 1585, 2182, 2485, 3319
BsiSI CCGG 5 cut(s) 849, 962, 1000, 1163, 1217
BslFI GGGAC 2 cut(s) 978, 2470
BsmAI GTCTC 5 cut(s) 498, 758, 1112, 2199, 2844
BsmFI GGGAC 2 cut(s) 978, 2470
BsnI GGCC 5 cut(s) 59, 720, 1954, 3020, 3190
Bso31I GGTCTC 2 cut(s) 1112, 2844
Bsp1286I GDGCHC 7 cut(s) 94, 205, 1270, 1585, 2182, 2485, 3319
Bsp1407I TGTACA 2 cut(s) 1224, 1479
Bsp143I GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
Bsp19I CCATGG 1 cut(s) 3026
BspACI CCGC 3 cut(s) 554, 721, 2905
BspANI GGCC 5 cut(s) 59, 720, 1954, 3020, 3190
BspCNI CTCAG 5 cut(s) 508, 531, 2854, 2954, 3230
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 3 cut(s) 91, 211, 2694
BspMAI CTGCAG 2 cut(s) 76, 508
BspPI GGATC 5 cut(s) 204, 217, 560, 2382, 2924
BspQI GCTCTTC 1 cut(s) 1017
BspT107I GGYRCC 2 cut(s) 89, 2692
BspTNI GGTCTC 2 cut(s) 1112, 2844
BsrDI GCAATG 5 cut(s) 581, 876, 2425, 2512, 3370
BsrFI RCCGGY 1 cut(s) 848
BsrGI TGTACA 2 cut(s) 1224, 1479
BsrI ACTGG 5 cut(s) 35, 181, 2464, 2526, 2856
BssAI RCCGGY 1 cut(s) 848
BssECI CCNNGG 4 cut(s) 1406, 2300, 3026, 3185
BssMI GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
BssT1I CCWWGG 3 cut(s) 1406, 2300, 3026
Bst2UI CCWGG 5 cut(s) 2451, 2562, 2584, 3186, 3279
Bst4CI ACNGT 7 cut(s) 276, 551, 1066, 1894, 1981, 2173, 3009
Bst6I CTCTTC 3 cut(s) 1017, 2875, 3033
BstAPI GCANNNNNTGC 2 cut(s) 527, 2393
BstAUI TGTACA 2 cut(s) 1224, 1479
BstBAI YACGTR 2 cut(s) 1928, 2183
BstC8I GCNNGC 4 cut(s) 152, 556, 1786, 2096
BstDEI CTNAG 9 cut(s) 495, 539, 776, 1057, 1323, 1761, 2841, 2941, 3217
BstDSI CCRYGG 1 cut(s) 3026
BstENI CCTNNNNNAGG 1 cut(s) 1905
BstF5I GGATG 6 cut(s) 619, 1171, 2128, 2275, 2320, 3232
BstFNI CGCG 1 cut(s) 417
BstKTI GATC 9 cut(s) 212, 568, 982, 2377, 2512, 2669, 2731, 2921, 2932
BstMAI GTCTC 5 cut(s) 498, 758, 1112, 2199, 2844
BstMBI GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
BstMWI GCNNNNNNNGC 5 cut(s) 527, 1589, 2393, 2489, 2720
BstNI CCWGG 5 cut(s) 2451, 2562, 2584, 3186, 3279
BstNSI RCATGY 6 cut(s) 158, 924, 1079, 1118, 1771, 2625
BstSCI CCNGG 8 cut(s) 961, 998, 1161, 2449, 2560, 2582, 3184, 3277
BstSFI CTRYAG 5 cut(s) 72, 486, 504, 2169, 2409
BstSLI GKGCMC 4 cut(s) 94, 1585, 2182, 2485
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 4 cut(s) 490, 1467, 2558, 3113
BstV2I GAAGAC 3 cut(s) 2004, 2349, 2997
BstX2I RGATCY 2 cut(s) 209, 2918
BstYI RGATCY 2 cut(s) 209, 2918
BsuI GTATCC 2 cut(s) 1641, 2021
BsuRI GGCC 5 cut(s) 59, 720, 1954, 3020, 3190
BtgI CCRYGG 1 cut(s) 3026
BtgZI GCGATG 1 cut(s) 2364
BtrI CACGTC 2 cut(s) 651, 812
BtsCI GGATG 6 cut(s) 619, 1171, 2128, 2275, 2320, 3232
BtsIMutI CAGTG 4 cut(s) 547, 1644, 2182, 2679
Cac8I GCNNGC 4 cut(s) 152, 556, 1786, 2096
CaiI CAGNNNCTG 1 cut(s) 3191
Cfr10I RCCGGY 1 cut(s) 848
Cfr13I GGNCC 6 cut(s) 880, 1256, 1952, 2447, 2848, 3188
DdeI CTNAG 9 cut(s) 495, 539, 776, 1057, 1323, 1761, 2841, 2941, 3217
DpnI GATC 9 cut(s) 211, 567, 981, 2376, 2511, 2668, 2730, 2920, 2931
DpnII GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
DraI TTTAAA 2 cut(s) 2590, 2740
EaeI YGGCCR 2 cut(s) 57, 3018
Eam1104I CTCTTC 3 cut(s) 1017, 2875, 3033
EarI CTCTTC 3 cut(s) 1017, 2875, 3033
Eco130I CCWWGG 3 cut(s) 1406, 2300, 3026
Eco31I GGTCTC 2 cut(s) 1112, 2844
Eco47I GGWCC 4 cut(s) 880, 1256, 2447, 2848
Eco57I CTGAAG 3 cut(s) 1049, 1620, 1887
EcoNI CCTNNNNNAGG 1 cut(s) 1905
EcoO109I RGGNCCY 2 cut(s) 1952, 3188
EcoRII CCWGG 5 cut(s) 2449, 2560, 2582, 3184, 3277
EcoT14I CCWWGG 3 cut(s) 1406, 2300, 3026
EcoT22I ATGCAT 3 cut(s) 865, 2614, 2706
ErhI CCWWGG 3 cut(s) 1406, 2300, 3026
FalI AAGNNNNNCTT 2 cut(s) 1907, 1939
FaqI GGGAC 2 cut(s) 978, 2470
FauI CCCGC 1 cut(s) 547
FauNDI CATATG 4 cut(s) 604, 865, 1122, 2046
FbaI TGATCA 1 cut(s) 2509
FblI GTMKAC 2 cut(s) 159, 1491
Fnu4HI GCNGC 6 cut(s) 504, 721, 1456, 2547, 2906, 3102
FokI GGATG 6 cut(s) 626, 1178, 2135, 2282, 2327, 3239
Fsp4HI GCNGC 6 cut(s) 504, 721, 1456, 2547, 2906, 3102
FspBI CTAG 5 cut(s) 170, 407, 1376, 2301, 3234
GluI GCNGC 6 cut(s) 504, 721, 1456, 2547, 2906, 3102
GsuI CTGGAG 3 cut(s) 18, 33, 2566
HaeIII GGCC 5 cut(s) 59, 720, 1954, 3020, 3190
HapII CCGG 5 cut(s) 849, 962, 1000, 1163, 1217
HincII GTYRAC 4 cut(s) 160, 2866, 3151, 3300
HindII GTYRAC 4 cut(s) 160, 2866, 3151, 3300
HindIII AAGCTT 1 cut(s) 773
HpaI GTTAAC 3 cut(s) 2866, 3151, 3300
HpaII CCGG 5 cut(s) 849, 962, 1000, 1163, 1217
HphI GGTGA 5 cut(s) 395, 863, 2294, 3047, 3264
Hpy99I CGWCG 2 cut(s) 746, 813
HpyAV CCTTC 6 cut(s) 1024, 1644, 1772, 1901, 1911, 2061
HpyCH4III ACNGT 7 cut(s) 276, 551, 1066, 1894, 1981, 2173, 3009
HpyCH4IV ACGT 7 cut(s) 469, 650, 811, 1102, 1927, 2182, 3153
HpyF10VI GCNNNNNNNGC 5 cut(s) 527, 1589, 2393, 2489, 2720
HpyF3I CTNAG 9 cut(s) 495, 539, 776, 1057, 1323, 1761, 2841, 2941, 3217
HpySE526I ACGT 7 cut(s) 469, 650, 811, 1102, 1927, 2182, 3153
Ksp22I TGATCA 1 cut(s) 2509
KspAI GTTAAC 3 cut(s) 2866, 3151, 3300
Kzo9I GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 2 cut(s) 1265, 3322
Lsp1109I GCAGC 4 cut(s) 490, 1467, 2558, 3113
LweI GCATC 8 cut(s) 517, 1555, 1731, 2341, 2599, 2621, 2691, 3088
MaeI CTAG 5 cut(s) 170, 407, 1376, 2301, 3234
MaeII ACGT 7 cut(s) 469, 650, 811, 1102, 1927, 2182, 3153
MaeIII GTNAC 7 cut(s) 545, 656, 726, 923, 1510, 1702, 2074
MalI GATC 9 cut(s) 211, 567, 981, 2376, 2511, 2668, 2730, 2920, 2931
MboI GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
MfeI CAATTG 1 cut(s) 3179
MflI RGATCY 2 cut(s) 209, 2918
MhlI GDGCHC 7 cut(s) 94, 205, 1270, 1585, 2182, 2485, 3319
MlsI TGGCCA 1 cut(s) 3020
MluNI TGGCCA 1 cut(s) 3020
MlyI GAGTC 8 cut(s) 432, 500, 822, 830, 1352, 2190, 3050, 3209
MmeI TCCRAC 3 cut(s) 605, 2038, 2827
Mox20I TGGCCA 1 cut(s) 3020
Mph1103I ATGCAT 3 cut(s) 865, 2614, 2706
MroXI GAANNNNTTC 1 cut(s) 3069
MscI TGGCCA 1 cut(s) 3020
MslI CAYNNNNRTG 7 cut(s) 143, 339, 607, 1080, 1437, 2045, 3093
Msp20I TGGCCA 1 cut(s) 3020
MspI CCGG 5 cut(s) 849, 962, 1000, 1163, 1217
MspR9I CCNGG 8 cut(s) 963, 1000, 1163, 2451, 2562, 2584, 3186, 3279
MunI CAATTG 1 cut(s) 3179
MvaI CCWGG 5 cut(s) 2451, 2562, 2584, 3186, 3279
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 5 cut(s) 527, 1589, 2393, 2489, 2720
NciI CCSGG 3 cut(s) 963, 1000, 1163
NcoI CCATGG 1 cut(s) 3026
NdeI CATATG 4 cut(s) 604, 865, 1122, 2046
NdeII GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
NlaIV GGNNCC 3 cut(s) 91, 211, 2694
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 3 cut(s) 545, 656, 2074
NsiI ATGCAT 3 cut(s) 865, 2614, 2706
NspI RCATGY 6 cut(s) 158, 924, 1079, 1118, 1771, 2625
PciI ACATGT 4 cut(s) 920, 1075, 1114, 1767
PciSI GCTCTTC 1 cut(s) 1017
PdmI GAANNNNTTC 1 cut(s) 3069
PflMI CCANNNNNTGG 1 cut(s) 3027
PfoI TCCNGGA 1 cut(s) 2449
PkrI GCNGC 6 cut(s) 505, 722, 1457, 2548, 2907, 3103
PleI GAGTC 8 cut(s) 432, 499, 822, 830, 1352, 2190, 3049, 3209
PpsI GAGTC 8 cut(s) 432, 499, 822, 830, 1352, 2190, 3049, 3209
Ppu21I YACGTR 2 cut(s) 1928, 2183
PscI ACATGT 4 cut(s) 920, 1075, 1114, 1767
PsiI TTATAA 3 cut(s) 120, 822, 3173
Psp6I CCWGG 5 cut(s) 2449, 2560, 2582, 3184, 3277
PspGI CCWGG 5 cut(s) 2449, 2560, 2582, 3184, 3277
PspN4I GGNNCC 3 cut(s) 91, 211, 2694
PspPI GGNCC 6 cut(s) 880, 1256, 1952, 2447, 2848, 3188
PsrI GAACNNNNNNTAC 2 cut(s) 1695, 1727
PstI CTGCAG 2 cut(s) 76, 508
PstNI CAGNNNCTG 1 cut(s) 3191
PsuI RGATCY 2 cut(s) 209, 2918
RseI CAYNNNNRTG 7 cut(s) 143, 339, 607, 1080, 1437, 2045, 3093
SalI GTCGAC 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 1017
SatI GCNGC 6 cut(s) 504, 721, 1456, 2547, 2906, 3102
Sau3AI GATC 9 cut(s) 209, 565, 979, 2374, 2509, 2666, 2728, 2918, 2929
Sau96I GGNCC 6 cut(s) 880, 1256, 1952, 2447, 2848, 3188
SchI GAGTC 8 cut(s) 432, 500, 822, 830, 1352, 2190, 3050, 3209
ScrFI CCNGG 8 cut(s) 963, 1000, 1163, 2451, 2562, 2584, 3186, 3279
SduI GDGCHC 7 cut(s) 94, 205, 1270, 1585, 2182, 2485, 3319
SfaNI GCATC 8 cut(s) 517, 1555, 1731, 2341, 2599, 2621, 2691, 3088
SfcI CTRYAG 5 cut(s) 72, 486, 504, 2169, 2409
SinI GGWCC 4 cut(s) 880, 1256, 2447, 2848
SmiMI CAYNNNNRTG 7 cut(s) 143, 339, 607, 1080, 1437, 2045, 3093
SmlI CTYRAG 3 cut(s) 204, 2337, 2497
SmoI CTYRAG 3 cut(s) 204, 2337, 2497
SsiI CCGC 3 cut(s) 554, 721, 2905
SspI AATATT 2 cut(s) 1605, 3340
SspMI CTAG 5 cut(s) 170, 407, 1376, 2301, 3234
StyD4I CCNGG 8 cut(s) 961, 998, 1161, 2449, 2560, 2582, 3184, 3277
StyI CCWWGG 3 cut(s) 1406, 2300, 3026
TaaI ACNGT 7 cut(s) 276, 551, 1066, 1894, 1981, 2173, 3009
TaiI ACGT 7 cut(s) 472, 653, 814, 1105, 1930, 2185, 3156
TaqI TCGA 8 cut(s) 159, 712, 744, 1186, 1443, 1525, 1801, 3213
TatI WGTACW 4 cut(s) 1224, 1479, 2518, 3204
TauI GCSGC 2 cut(s) 723, 2908
TscAI CASTG 4 cut(s) 554, 1644, 2182, 2679
TseFI GTSAC 3 cut(s) 545, 656, 2074
TseI GCWGC 4 cut(s) 503, 1455, 2546, 3101
Tsp45I GTSAC 3 cut(s) 545, 656, 2074
TspGWI ACGGA 3 cut(s) 372, 1386, 2229
TspRI CASTG 4 cut(s) 554, 1644, 2182, 2679
Van91I CCANNNNNTGG 1 cut(s) 3027
VneI GTGCAC 3 cut(s) 1581, 2178, 2481
VpaK11BI GGWCC 4 cut(s) 880, 1256, 2447, 2848
XagI CCTNNNNNAGG 1 cut(s) 1905
XapI RAATTY 9 cut(s) 903, 1016, 1091, 1555, 1610, 1653, 2126, 2592, 3262
XceI RCATGY 6 cut(s) 158, 924, 1079, 1118, 1771, 2625
XcmI CCANNNNNNNNNTGG 2 cut(s) 1593, 1874
XmaJI CCTAGG 1 cut(s) 2300
XmiI GTMKAC 2 cut(s) 159, 1491
XmnI GAANNNNTTC 1 cut(s) 3069
XspI CTAG 5 cut(s) 170, 407, 1376, 2301, 3234
Zsp2I ATGCAT 3 cut(s) 865, 2614, 2706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.