Rw4G008860

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000080
Physical Location & Seq
Reverse (-)
19544841 .. 19545639
799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008860.1

Sequence Viewer

Length: 534 bp
ATGTTAGAGGGTTCCATTGATAGCAGTATTCAAGTGAGAGCATCACTACTAAAATCAGACAAGAGGAAGCAGTTAATATCACAGGGTGGAGTTGATAATGGGTGCATGAACAATTTACTTGGATGTGAGTTGCAAGCTGGGACAAGTAAAATTGGCCAAGCCTCATATCAAAAAAGTATTCAAGGGACTGTGCATGATTACAGAGATGTTGGGGACATGATATATTGTTGTTGTTATTGCAACGCACTGTTCTGGAGAAATGAATCAAAGAAACAGAGGAGCAAGAATAAACAGCCAATTTTTACACATTGCTGCCAAGAGGGCAAGATATGCCTGCCACCAGAGCCTGACACTCCGGAATTTCTAAATAACTTATTGGATCCTTCAAAGAATTCATTGTTTAGAGCTAATATTTGTATTGACAACTCAATGTTCAGTTTCACTTATATGGGTGGAAAAGTTGATCATTCTGTTAATAATGGTTCCGGACCTTATGTCTTTCGAATTAGTGGTCAAGTATGCAATTTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

177

Amino Acids

19.7

Weight (kDa)

8.27

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 355, 485
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 2 cut(s) 359, 391
AdeI CACNNNGTG 1 cut(s) 86
AgsI TTSAA 3 cut(s) 32, 182, 387
AhdI GACNNNNNGTC 1 cut(s) 494
AluBI AGCT 2 cut(s) 137, 407
AluI AGCT 2 cut(s) 137, 407
AlwI GGATC 2 cut(s) 374, 387
AlwNI CAGNNNCTG 1 cut(s) 347
Aor13HI TCCGGA 2 cut(s) 355, 485
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 2 cut(s) 359, 391
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
AspS9I GGNCC 1 cut(s) 488
AsuII TTCGAA 1 cut(s) 502
AvaII GGWCC 1 cut(s) 488
BalI TGGCCA 1 cut(s) 156
BamHI GGATCC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 299
BclI TGATCA 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 321
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
Bme18I GGWCC 1 cut(s) 488
BmeRI GACNNNNNGTC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 488
BmiI GGNNCC 3 cut(s) 13, 381, 484
BmsI GCATC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 274
Bpu14I TTCGAA 1 cut(s) 502
BsaWI WCCGGW 2 cut(s) 355, 485
Bse3DI GCAATG 1 cut(s) 307
BseAI TCCGGA 2 cut(s) 355, 485
BseGI GGATG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 299
BseYI CCCAGC 1 cut(s) 137
BshFI GGCC 1 cut(s) 156
BsiSI CCGG 2 cut(s) 356, 486
BslFI GGGAC 3 cut(s) 154, 199, 227
BsmFI GGGAC 3 cut(s) 154, 199, 227
BsnI GGCC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 502
Bsp13I TCCGGA 2 cut(s) 355, 485
Bsp143I GATC 2 cut(s) 379, 463
BspANI GGCC 1 cut(s) 156
BspEI TCCGGA 2 cut(s) 355, 485
BspLI GGNNCC 3 cut(s) 13, 381, 484
BspPI GGATC 2 cut(s) 374, 387
BspT104I TTCGAA 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 307
BssMI GATC 2 cut(s) 379, 463
Bst4CI ACNGT 2 cut(s) 190, 249
BstAPI GCANNNNNTGC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 502
BstC8I GCNNGC 2 cut(s) 135, 335
BstF5I GGATG 1 cut(s) 128
BstKTI GATC 2 cut(s) 382, 466
BstMBI GATC 2 cut(s) 379, 463
BstMWI GCNNNNNNNGC 3 cut(s) 321, 330, 343
BstV1I GCAGC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 1 cut(s) 156
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 135, 335
CaiI CAGNNNCTG 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 488
CviAII CATG 3 cut(s) 106, 194, 217
CviJI RGCY 6 cut(s) 137, 156, 161, 295, 346, 407
CviKI_1 RGCY 6 cut(s) 137, 156, 161, 295, 346, 407
DpnI GATC 2 cut(s) 381, 465
DpnII GATC 2 cut(s) 379, 463
DraIII CACNNNGTG 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 494
EaeI YGGCCR 1 cut(s) 154
Eam1105I GACNNNNNGTC 1 cut(s) 494
Eco47I GGWCC 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 391
FaeI CATG 3 cut(s) 109, 197, 220
FaqI GGGAC 3 cut(s) 154, 199, 227
FatI CATG 3 cut(s) 105, 193, 216
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 1 cut(s) 313
FokI GGATG 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 313
GluI GCNGC 1 cut(s) 313
GsaI CCCAGC 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 1 cut(s) 156
HapII CCGG 2 cut(s) 356, 486
Hin1II CATG 3 cut(s) 109, 197, 220
HinfI GANTC 1 cut(s) 263
HpaII CCGG 2 cut(s) 356, 486
Hpy188I TCNGA 1 cut(s) 58
Hpy188III TCNNGA 3 cut(s) 253, 356, 486
HpyAV CCTTC 1 cut(s) 393
HpyCH4III ACNGT 2 cut(s) 190, 249
HpyCH4V TGCA 5 cut(s) 105, 133, 193, 240, 522
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 330, 343
Hsp92II CATG 3 cut(s) 109, 197, 220
Kpn2I TCCGGA 2 cut(s) 355, 485
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 2 cut(s) 379, 463
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 8 cut(s) 68, 123, 238, 347, 354, 360, 369, 499
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 1 cut(s) 50
MalI GATC 2 cut(s) 381, 465
MboI GATC 2 cut(s) 379, 463
MflI RGATCY 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 7 cut(s) 112, 150, 297, 359, 391, 504, 523
MluNI TGGCCA 1 cut(s) 156
MnlI CCTC 4 cut(s) 57, 172, 270, 313
Mox20I TGGCCA 1 cut(s) 156
MroI TCCGGA 2 cut(s) 355, 485
MscI TGGCCA 1 cut(s) 156
MseI TTAA 3 cut(s) 74, 474, 527
MslI CAYNNNNRTG 1 cut(s) 446
Msp20I TGGCCA 1 cut(s) 156
MspI CCGG 2 cut(s) 356, 486
MwoI GCNNNNNNNGC 3 cut(s) 321, 330, 343
NdeII GATC 2 cut(s) 379, 463
NlaIII CATG 3 cut(s) 109, 197, 220
NlaIV GGNNCC 3 cut(s) 13, 381, 484
NspV TTCGAA 1 cut(s) 502
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 137
PspN4I GGNNCC 3 cut(s) 13, 381, 484
PspPI GGNCC 1 cut(s) 488
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 3 cut(s) 74, 474, 527
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 2 cut(s) 379, 463
Sau96I GGNCC 1 cut(s) 488
SetI ASST 3 cut(s) 139, 409, 493
SfaNI GCATC 1 cut(s) 50
SfuI TTCGAA 1 cut(s) 502
SinI GGWCC 1 cut(s) 488
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 7 cut(s) 112, 150, 297, 359, 391, 504, 523
SspI AATATT 1 cut(s) 412
TaaI ACNGT 2 cut(s) 190, 249
TaqI TCGA 1 cut(s) 502
TasI AATT 7 cut(s) 112, 150, 297, 359, 391, 504, 523
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 74, 474, 527
Tru9I TTAA 3 cut(s) 74, 474, 527
TscAI CASTG 1 cut(s) 252
TseI GCWGC 1 cut(s) 312
TspDTI ATGAA 3 cut(s) 122, 276, 384
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 488
XapI RAATTY 2 cut(s) 359, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.