Rw5G028940

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000080
Physical Location & Seq
Reverse (-)
43990968 .. 43993872
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028940.1

Sequence Viewer

Length: 2865 bp
ATGGCTTTAGTCCAAAAATATGGAAAACCGGATATTTTTCTCACAATGACATGTAATCCAAATTGGGAAGAAATAACTGCAGAGTTATTACCTGGTCAAGTGGCACAAGATCGCCCGGATCTAACTACTCGAATTTTTCATGCCAAATTTGAAGAGTTAAAGAATGATGTTATTAAAAAAGGAGTCTTGGGTACTATTGTTGCATACGTATATGTCATCGAGTTTCAAAAACGTGGTCTCCCACATGTTCATATGCTTCTCATGTTGAGTGACGATGATAAACTAAACAACCCAGATGATTATGACCACATTGTTCGGGCTGAAATTCCAAATCAAAATGAAGAGCCAGAGTTATACAATGCAGTGTTGGGGCACATGATACACGGTCCATGTGGGGTACATAGGCGAACTGCTCCATGTATGAAGCGTGGGAGCTGCAAACGAGGCTATCCAAAGCCATTTTCAGTAACCACATTTCAAGGAAATGATTCTTATCCTGTTTATCGTAGACGAGATAATCAATCAGATATGCAATCTAATGGCAATCAAAATAGACCACTGGATAATAGTTGGGTTATCCCCTATAATCCATGGTTACTCACAAAATATGATTGTCATATCAATGTGGAAATTTGTTGCAGCATTAAGAGCGTCAAGTATTTGTATAAATATGTCTACAAAGGTCCAGACAGAGTAACATTTGAAGTTCGGCCTGAAGCAAATCAAGATGAAATAAGAAACTTTGTTGATGCAAGATGGGTTTGTGCACCAGAAGCTTTGTGGAGGATGTTTAAATTCGTGATGAATCGAATGTATCCATCAGTGGAACGATTGCAAATTCATCTTCCAAATCGACACAATGTTTTCTTTAATGCCACTGAAAGTGTAACAGCAATTCTGGCAAATGAAAGAAGCTCGATGACAATGCTCACAGAATTTTTTACTAAGAATGCTTATTTTGAAAGTGCACGTCAATACTTATATCGAGAGTTTCCTGAGCATTACAAATGGGATGGGAGACTTAAAACATGGGAAGCAAGGGAGACCAACCAAAAAGTCATTGGAAGAATTTACACAGTGTCACCTTCAGAAGGAGAAAAGTTTTACCTGCGTGTTCTTCTTAATCATGTTCGAGGACCTAAGTCATTTGAGGATTTAAGGACAGTTGATGGGGTCTTACAACCGACATTCAAGAAAGCAGTTGAAGAACGTGGTTTGTTAGAAGATGATGAAAGTATTAGACAATGTCTACGTGAGGCCTCTAATATACGTATGCCGTCATCTTTAAGAAGATTGTTTGTTACCATCCTGGTTTATTGCATGCCCCATGGAGTACGCAGTTTGTGGGATGAATTTTATCCATTCATGATAGAGGATTATCCTTCTTCGACCACCACGGACAATGTACGTATCACAAATAGACTTTTACGGGACTTGAACGAGTTGTTGGTGCAGCACAGTAAGTCCGTCTTTGATTATGACTTGCCAGAAATGACTCAAGATGGTGGTGAAAATTCTTCAATGCCAAGGCTAATACAAGATGAAATTTCAATTAACATTCCTCAAGAAGACCGGGATGCAGTTCATCATTTGAATGAGGATCAAACTTTTGCATACAATTCCATAATATCTGCTATTGAGTGTCATGACAATGCCATATTTTTTGTTGATGGTCCAGGAGGAACTGGAAAGACATACTTATATCGTGCATTATTAGCAAGCTTGAGAAGCAATGATCATATTGTATTGGCAACAGCAACTTCTGGCATAGCAGCAATAATACTACCTGGTGGGAGGACGGCACACTCTAGGTTTAAAATTCCTCTTAATCTTGATGCATCTTCAACATGTTGTATTAGTAAACAATCTGATTTAGCAGAGTTGATAAGAAAGTCATCGGCAATTGTTTGGGATGAAGCACCAATGATGAATCGATATGGGTTTGAAGCTCTTGATCGAACATTTAGAGACATAACAAGTGTCGATTTGCCATTTGGAGGAAAGGTGATGATCTTCGGGGGTGATTTCCGACAAGTTCTTCCTGTTATCCATAAGGGTACGAGATCTGAGATGGTGCAAGCCAGTTTGATTAATGCTTCATTTTGGAAGGATGTGAAGATTCTTCGTTTGACACAGAATATGAGATCCATCAACGATCCAGATTTCTCAGAGTTCTTACTTCGTGTTGGTAATGGAGAACAACCAACTGTGGTTGAAGACATAATACAAATACCTTGGCCCATGATCATACCATGGGAGAATGAAGAATCAATCAACCAGTTGGTTAATCAAGTCTTCCCTAATTTGGATCGTCATGTAAATGATGCCGGGTACATGGTTGAAAGAGCAATAATTACTCCAAAAAATGATGATGTTGACGTACTCAATGAAAGAATCATACAACACTTTCCAGGGCCTGAACGAATCTTATACTCTTTCGACTCAGTTGAGGATGACACCAGAAATATGTATCAACAAGAATTTTTAAATTCGATTTCACCTTCTGGTATGCCGCCACATCAGTTGATTATAAAGAAAGGTGCCCCAATTATGCTTTTGAGGAATATTGACCCAAAGATGGGGTTGTGCAACGGTACAAGATTGACTTGCCGCGGAGCATATAACAATCTAATTGATGCAGAGATTTTAACAGGACATTTTTCGGGAACTAGAATTTTCTTGCCAAGAATCGCTCTTAAGAGTGCCGAAAGTTCTGGACTCCCGTTTGAGATGACAAGAAAGCAGTTCCCCGTAAAACAATACCAAATGTTGGTATTTACCTTCCTGATCATGTGTTCAGCCATGGACAACTGTACGTGGCTTTATCGAGAGGAGTTTCAGAGGCAAGCACCAAAGTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

954

Amino Acids

110.09

Weight (kDa)

6.16

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 1 - 87 1.2e-35 Helitron helicase-like domain at N-terminus
REP_ORF2-G2P PF23343 12 - 99 8.5e-06 Replication-associated protein ORF2/G2P
PIF1 PF05970 531 - 736 6e-76 PIF1-like helicase
Pif1_2B_dom PF21530 827 - 869 1.2e-19 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2531
Acc36I ACCTGC 1 cut(s) 1116
AccB1I GGYRCC 1 cut(s) 2540
AccB7I CCANNNNNTGG 1 cut(s) 2770
AccI GTMKAC 3 cut(s) 508, 675, 1249
AccII CGCG 1 cut(s) 2613
AciI CCGC 3 cut(s) 2513, 2611, 2613
AclWI GGATC 5 cut(s) 126, 1608, 2139, 2150, 2316
AcuI CTGAAG 2 cut(s) 735, 1071
AfaI GTAC 9 cut(s) 193, 399, 1335, 1407, 2059, 2333, 2382, 2596, 2815
AfiI CCNNNNNNNGG 6 cut(s) 1091, 1997, 2305, 2578, 2579, 2770
AflII CTTAAG 1 cut(s) 2696
AflIII ACRYGT 3 cut(s) 50, 244, 1847
AjiI CACGTC 1 cut(s) 971
AjnI CCWGG 5 cut(s) 91, 1308, 1675, 1786, 2410
AjuI GAANNNNNNNTTGG 4 cut(s) 445, 477, 1430, 1462
AluBI AGCT 5 cut(s) 435, 776, 915, 1722, 1949
AluI AGCT 5 cut(s) 435, 776, 915, 1722, 1949
Alw21I GWGCWC 2 cut(s) 769, 970
Alw26I GTCTC 4 cut(s) 242, 1012, 1037, 1962
Alw44I GTGCAC 2 cut(s) 765, 966
AlwI GGATC 5 cut(s) 126, 1608, 2139, 2150, 2316
AlwNI CAGNNNCTG 1 cut(s) 2417
AoxI GGCC 4 cut(s) 710, 1257, 2237, 2414
ApaLI GTGCAC 2 cut(s) 765, 966
ApeKI GCWGC 4 cut(s) 435, 639, 1453, 1772
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
AseI ATTAAT 1 cut(s) 2091
Asp700I GAANNNNTTC 1 cut(s) 487
AspS9I GGNCC 6 cut(s) 386, 683, 1136, 1673, 2238, 2414
AsuC2I CCSGG 3 cut(s) 116, 1574, 2329
AsuHPI GGTGA 5 cut(s) 1074, 1520, 2017, 2033, 2490
AvaII GGWCC 4 cut(s) 386, 683, 1136, 1673
BaeGI GKGCMC 4 cut(s) 375, 769, 970, 2545
BaeI ACNNNNGTAYC 2 cut(s) 183, 216
BanI GGYRCC 1 cut(s) 2540
BarI GAAGNNNNNNTAC 2 cut(s) 2208, 2240
BbsI GAAGAC 3 cut(s) 1575, 2223, 2287
Bbv12I GWGCWC 2 cut(s) 769, 970
BbvI GCAGC 4 cut(s) 422, 651, 1465, 1784
BceAI ACGGC 2 cut(s) 1261, 1815
BcgI CGANNNNNNTGC 2 cut(s) 907, 941
BciT130I CCWGG 5 cut(s) 93, 1310, 1677, 1788, 2412
BciVI GTATCC 1 cut(s) 825
BclI TGATCA 3 cut(s) 1735, 2244, 2787
BcnI CCSGG 3 cut(s) 116, 1574, 2329
BcoDI GTCTC 4 cut(s) 242, 1012, 1037, 1962
BfaI CTAG 2 cut(s) 1809, 2670
BfmI CTRYAG 1 cut(s) 78
BfrI CTTAAG 1 cut(s) 2696
BfuAI ACCTGC 1 cut(s) 1116
BfuI GTATCC 1 cut(s) 825
BglII AGATCT 1 cut(s) 2063
BisI GCNGC 6 cut(s) 436, 640, 1454, 1773, 2513, 2611
BlsI GCNGC 6 cut(s) 437, 641, 1455, 1774, 2514, 2612
Bme1390I CCNGG 8 cut(s) 93, 116, 1310, 1574, 1677, 1788, 2329, 2412
Bme18I GGWCC 4 cut(s) 386, 683, 1136, 1673
BmgBI CACGTC 1 cut(s) 971
BmgT120I GGNCC 6 cut(s) 386, 683, 1136, 1673, 2238, 2414
BmiI GGNNCC 1 cut(s) 2542
BmrFI CCNGG 8 cut(s) 93, 116, 1310, 1574, 1677, 1788, 2329, 2412
BmsI GCATC 6 cut(s) 739, 1567, 1825, 1847, 2314, 2626
BoxI GACNNNNGTC 1 cut(s) 1141
BpiI GAAGAC 3 cut(s) 1575, 2223, 2287
Bpu10I CCTNAGC 1 cut(s) 996
BpuEI CTTGAG 3 cut(s) 1482, 1548, 1744
BpuMI CCSGG 3 cut(s) 116, 1574, 2329
Bsa29I ATCGAT 1 cut(s) 1933
BsaAI YACGTR 5 cut(s) 208, 1253, 1271, 1409, 2817
BsaBI GATNNNNATC 1 cut(s) 492
BsaI GGTCTC 2 cut(s) 242, 1037
BsaJI CCNNGG 9 cut(s) 590, 1327, 1395, 1526, 2234, 2252, 2411, 2611, 2802
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 1989, 2019
Bsc4I CCNNNNNNNGG 6 cut(s) 1091, 1997, 2305, 2578, 2579, 2770
Bse1I ACTGG 4 cut(s) 564, 1690, 2082, 2278
Bse3DI GCAATG 1 cut(s) 1738
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 5 cut(s) 93, 1310, 1677, 1788, 2412
BseCI ATCGAT 1 cut(s) 1933
BseDI CCNNGG 9 cut(s) 590, 1327, 1395, 1526, 2234, 2252, 2411, 2611, 2802
BseGI GGATG 8 cut(s) 792, 1018, 1305, 1354, 1582, 1918, 2116, 2458
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 6 cut(s) 1091, 1997, 2305, 2578, 2579, 2770
BseMI GCAATG 1 cut(s) 1738
BseMII CTCAG 4 cut(s) 987, 2058, 2181, 2457
BseNI ACTGG 4 cut(s) 564, 1690, 2082, 2278
BseRI GAGGAG 1 cut(s) 2846
BseSI GKGCMC 4 cut(s) 375, 769, 970, 2545
BseXI GCAGC 4 cut(s) 422, 651, 1465, 1784
BsgI GTGCAG 1 cut(s) 1472
Bsh1236I CGCG 1 cut(s) 2613
BshFI GGCC 4 cut(s) 712, 1259, 2239, 2416
BshNI GGYRCC 1 cut(s) 2540
BshVI ATCGAT 1 cut(s) 1933
BsiHKAI GWGCWC 2 cut(s) 769, 970
BsiSI CCGG 4 cut(s) 29, 116, 1573, 2328
BslFI GGGAC 1 cut(s) 1445
BslI CCNNNNNNNGG 6 cut(s) 1091, 1997, 2305, 2578, 2579, 2770
BsmAI GTCTC 4 cut(s) 242, 1012, 1037, 1962
BsmFI GGGAC 1 cut(s) 1445
BsmI GAATGC 1 cut(s) 955
BsnI GGCC 4 cut(s) 712, 1259, 2239, 2416
Bso31I GGTCTC 2 cut(s) 242, 1037
Bsp1286I GDGCHC 4 cut(s) 375, 769, 970, 2545
Bsp19I CCATGG 4 cut(s) 590, 1327, 2252, 2802
BspACI CCGC 3 cut(s) 2513, 2611, 2613
BspANI GGCC 4 cut(s) 712, 1259, 2239, 2416
BspCNI CTCAG 4 cut(s) 988, 2059, 2180, 2456
BspDI ATCGAT 1 cut(s) 1933
BspFNI CGCG 1 cut(s) 2613
BspHI TCATGA 2 cut(s) 1365, 1645
BspLI GGNNCC 1 cut(s) 2542
BspMAI CTGCAG 1 cut(s) 82
BspMI ACCTGC 1 cut(s) 1116
BspPI GGATC 5 cut(s) 126, 1608, 2139, 2150, 2316
BspQI GCTCTTC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 2540
BspTI CTTAAG 1 cut(s) 2696
BspTNI GGTCTC 2 cut(s) 242, 1037
BsrDI GCAATG 1 cut(s) 1738
BsrI ACTGG 4 cut(s) 564, 1690, 2082, 2278
BssECI CCNNGG 9 cut(s) 590, 1327, 1395, 1526, 2234, 2252, 2411, 2611, 2802
BssT1I CCWWGG 6 cut(s) 590, 1327, 1526, 2234, 2252, 2802
Bst2UI CCWGG 5 cut(s) 93, 1310, 1677, 1788, 2412
Bst4CI ACNGT 7 cut(s) 386, 1078, 1165, 1460, 2209, 2594, 2813
Bst6I CTCTTC 2 cut(s) 147, 336
BstAFI CTTAAG 1 cut(s) 2696
BstBAI YACGTR 5 cut(s) 208, 1253, 1271, 1409, 2817
BstC8I GCNNGC 4 cut(s) 1322, 1720, 2079, 2847
BstDEI CTNAG 6 cut(s) 945, 996, 1140, 2067, 2167, 2443
BstDSI CCRYGG 6 cut(s) 590, 1327, 1395, 2252, 2611, 2802
BstENI CCTNNNNNAGG 1 cut(s) 1089
BstF5I GGATG 8 cut(s) 792, 1018, 1305, 1354, 1582, 1918, 2116, 2458
BstFNI CGCG 1 cut(s) 2613
BstMAI GTCTC 4 cut(s) 242, 1012, 1037, 1962
BstMWI GCNNNNNNNGC 6 cut(s) 444, 648, 773, 899, 1715, 1728
BstNI CCWGG 5 cut(s) 93, 1310, 1677, 1788, 2412
BstNSI RCATGY 4 cut(s) 54, 248, 1324, 1851
BstPAI GACNNNNGTC 1 cut(s) 1141
BstSCI CCNGG 8 cut(s) 91, 114, 1308, 1572, 1675, 1786, 2327, 2410
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 4 cut(s) 375, 769, 970, 2545
BstSNI TACGTA 3 cut(s) 208, 1271, 1409
BstUI CGCG 1 cut(s) 2613
BstV1I GCAGC 4 cut(s) 422, 651, 1465, 1784
BstV2I GAAGAC 3 cut(s) 1575, 2223, 2287
BstX2I RGATCY 3 cut(s) 118, 2063, 2144
BstXI CCANNNNNNTGG 2 cut(s) 20, 2859
BstYI RGATCY 3 cut(s) 118, 2063, 2144
Bsu15I ATCGAT 1 cut(s) 1933
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 4 cut(s) 712, 1259, 2239, 2416
BsuTUI ATCGAT 1 cut(s) 1933
BtgI CCRYGG 6 cut(s) 590, 1327, 1395, 2252, 2611, 2802
BtrI CACGTC 1 cut(s) 971
BtsCI GGATG 8 cut(s) 792, 1018, 1305, 1354, 1582, 1918, 2116, 2458
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 5 cut(s) 369, 557, 828, 876, 1083
BveI ACCTGC 1 cut(s) 1116
Cac8I GCNNGC 4 cut(s) 1322, 1720, 2079, 2847
CaiI CAGNNNCTG 1 cut(s) 2417
CciI TCATGA 2 cut(s) 1365, 1645
Cfr13I GGNCC 6 cut(s) 386, 683, 1136, 1673, 2238, 2414
Cfr42I CCGCGG 1 cut(s) 2614
ClaI ATCGAT 1 cut(s) 1933
CseI GACGC 1 cut(s) 640
CsiI ACCWGGT 2 cut(s) 91, 1786
Csp6I GTAC 9 cut(s) 192, 398, 1334, 1406, 2058, 2332, 2381, 2595, 2814
CspCI CAANNNNNGTGG 2 cut(s) 2505, 2540
CviQI GTAC 9 cut(s) 192, 398, 1334, 1406, 2058, 2332, 2381, 2595, 2814
DdeI CTNAG 6 cut(s) 945, 996, 1140, 2067, 2167, 2443
DraI TTTAAA 3 cut(s) 793, 1816, 2487
Eam1104I CTCTTC 2 cut(s) 147, 336
EarI CTCTTC 2 cut(s) 147, 336
Eco105I TACGTA 3 cut(s) 208, 1271, 1409
Eco130I CCWWGG 6 cut(s) 590, 1327, 1526, 2234, 2252, 2802
Eco147I AGGCCT 1 cut(s) 1259
Eco31I GGTCTC 2 cut(s) 242, 1037
Eco47I GGWCC 4 cut(s) 386, 683, 1136, 1673
Eco57I CTGAAG 2 cut(s) 735, 1071
EcoNI CCTNNNNNAGG 1 cut(s) 1089
EcoO109I RGGNCCY 2 cut(s) 1136, 2414
EcoRII CCWGG 5 cut(s) 91, 1308, 1675, 1786, 2410
EcoT14I CCWWGG 6 cut(s) 590, 1327, 1526, 2234, 2252, 2802
EcoT22I ATGCAT 1 cut(s) 1840
ErhI CCWWGG 6 cut(s) 590, 1327, 1526, 2234, 2252, 2802
FalI AAGNNNNNCTT 6 cut(s) 1454, 1486, 1682, 1714, 2590, 2622
FaqI GGGAC 1 cut(s) 1445
FauNDI CATATG 1 cut(s) 252
FbaI TGATCA 3 cut(s) 1735, 2244, 2787
FblI GTMKAC 3 cut(s) 508, 675, 1249
Fnu4HI GCNGC 6 cut(s) 436, 640, 1454, 1773, 2513, 2611
FokI GGATG 8 cut(s) 799, 1025, 1292, 1361, 1589, 1925, 2123, 2465
Fsp4HI GCNGC 6 cut(s) 436, 640, 1454, 1773, 2513, 2611
FspBI CTAG 2 cut(s) 1809, 2670
GluI GCNGC 6 cut(s) 436, 640, 1454, 1773, 2513, 2611
HaeIII GGCC 4 cut(s) 712, 1259, 2239, 2416
HapII CCGG 4 cut(s) 29, 116, 1573, 2328
HgaI GACGC 1 cut(s) 640
HincII GTYRAC 1 cut(s) 2377
HindII GTYRAC 1 cut(s) 2377
HindIII AAGCTT 2 cut(s) 774, 1720
HpaII CCGG 4 cut(s) 29, 116, 1573, 2328
HphI GGTGA 5 cut(s) 1074, 1520, 2017, 2033, 2490
Hpy166II GTNNAC 7 cut(s) 509, 676, 767, 968, 1250, 1862, 2377
Hpy188I TCNGA 7 cut(s) 526, 1090, 1870, 2030, 2068, 2170, 2841
Hpy8I GTNNAC 7 cut(s) 509, 676, 767, 968, 1250, 1862, 2377
HpyAV CCTTC 6 cut(s) 1085, 1095, 1392, 2101, 2511, 2791
HpyCH4III ACNGT 7 cut(s) 386, 1078, 1165, 1460, 2209, 2594, 2813
HpyCH4IV ACGT 9 cut(s) 207, 232, 970, 1210, 1252, 1270, 1408, 2379, 2816
HpyF10VI GCNNNNNNNGC 6 cut(s) 444, 648, 773, 899, 1715, 1728
HpyF3I CTNAG 6 cut(s) 945, 996, 1140, 2067, 2167, 2443
HpySE526I ACGT 9 cut(s) 207, 232, 970, 1210, 1252, 1270, 1408, 2379, 2816
Ksp22I TGATCA 3 cut(s) 1735, 2244, 2787
KspI CCGCGG 1 cut(s) 2614
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 3 cut(s) 418, 432, 2615
Lsp1109I GCAGC 4 cut(s) 422, 651, 1465, 1784
LweI GCATC 6 cut(s) 739, 1567, 1825, 1847, 2314, 2626
MabI ACCWGGT 2 cut(s) 91, 1786
MaeI CTAG 2 cut(s) 1809, 2670
MaeII ACGT 9 cut(s) 207, 232, 970, 1210, 1252, 1270, 1408, 2379, 2816
MaeIII GTNAC 7 cut(s) 269, 466, 594, 694, 886, 1080, 1300
MfeI CAATTG 1 cut(s) 1902
MflI RGATCY 3 cut(s) 118, 2063, 2144
MhlI GDGCHC 4 cut(s) 375, 769, 970, 2545
MlyI GAGTC 4 cut(s) 192, 1489, 2435, 2712
MmeI TCCRAC 1 cut(s) 2053
Mph1103I ATGCAT 1 cut(s) 1840
MroXI GAANNNNTTC 1 cut(s) 487
MslI CAYNNNNRTG 4 cut(s) 621, 1593, 1650, 2319
MspA1I CMGCKG 1 cut(s) 2613
MspCI CTTAAG 1 cut(s) 2696
MspI CCGG 4 cut(s) 29, 116, 1573, 2328
MspR9I CCNGG 8 cut(s) 93, 116, 1310, 1574, 1677, 1788, 2329, 2412
MunI CAATTG 1 cut(s) 1902
Mva1269I GAATGC 1 cut(s) 955
MvaI CCWGG 5 cut(s) 93, 1310, 1677, 1788, 2412
MvnI CGCG 1 cut(s) 2613
MwoI GCNNNNNNNGC 6 cut(s) 444, 648, 773, 899, 1715, 1728
NciI CCSGG 3 cut(s) 116, 1574, 2329
NcoI CCATGG 4 cut(s) 590, 1327, 2252, 2802
NdeI CATATG 1 cut(s) 252
NlaIV GGNNCC 1 cut(s) 2542
NmuCI GTSAC 2 cut(s) 269, 1080
NsiI ATGCAT 1 cut(s) 1840
NspI RCATGY 4 cut(s) 54, 248, 1324, 1851
PaeI GCATGC 1 cut(s) 1324
PagI TCATGA 2 cut(s) 1365, 1645
PceI AGGCCT 1 cut(s) 1259
PciI ACATGT 3 cut(s) 50, 244, 1847
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 1 cut(s) 955
PdmI GAANNNNTTC 1 cut(s) 487
PfeI GAWTC 8 cut(s) 488, 805, 1930, 2119, 2267, 2394, 2424, 2688
PflFI GACNNNGTC 1 cut(s) 1245
PflMI CCANNNNNTGG 1 cut(s) 2770
PfoI TCCNGGA 1 cut(s) 1675
PkrI GCNGC 6 cut(s) 437, 641, 1455, 1774, 2514, 2612
PleI GAGTC 4 cut(s) 191, 1489, 2435, 2712
PpsI GAGTC 4 cut(s) 191, 1489, 2435, 2712
Ppu21I YACGTR 5 cut(s) 208, 1253, 1271, 1409, 2817
PpuMI RGGWCCY 1 cut(s) 1136
PscI ACATGT 3 cut(s) 50, 244, 1847
PshAI GACNNNNGTC 1 cut(s) 1141
PshBI ATTAAT 1 cut(s) 2091
PsiI TTATAA 1 cut(s) 2531
Psp5II RGGWCCY 1 cut(s) 1136
Psp6I CCWGG 5 cut(s) 91, 1308, 1675, 1786, 2410
PspGI CCWGG 5 cut(s) 91, 1308, 1675, 1786, 2410
PspN4I GGNNCC 1 cut(s) 2542
PspPI GGNCC 6 cut(s) 386, 683, 1136, 1673, 2238, 2414
PspPPI RGGWCCY 1 cut(s) 1136
PstI CTGCAG 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 2417
PsuI RGATCY 3 cut(s) 118, 2063, 2144
PsyI GACNNNGTC 1 cut(s) 1245
RsaI GTAC 9 cut(s) 193, 399, 1335, 1407, 2059, 2333, 2382, 2596, 2815
RsaNI GTAC 9 cut(s) 192, 398, 1334, 1406, 2058, 2332, 2381, 2595, 2814
RseI CAYNNNNRTG 4 cut(s) 621, 1593, 1650, 2319
SacII CCGCGG 1 cut(s) 2614
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 6 cut(s) 436, 640, 1454, 1773, 2513, 2611
Sau96I GGNCC 6 cut(s) 386, 683, 1136, 1673, 2238, 2414
SchI GAGTC 4 cut(s) 192, 1489, 2435, 2712
ScrFI CCNGG 8 cut(s) 93, 116, 1310, 1574, 1677, 1788, 2329, 2412
SduI GDGCHC 4 cut(s) 375, 769, 970, 2545
SexAI ACCWGGT 2 cut(s) 91, 1786
SfaNI GCATC 6 cut(s) 739, 1567, 1825, 1847, 2314, 2626
SfcI CTRYAG 1 cut(s) 78
Sfr303I CCGCGG 1 cut(s) 2614
SgrBI CCGCGG 1 cut(s) 2614
SinI GGWCC 4 cut(s) 386, 683, 1136, 1673
SmiMI CAYNNNNRTG 4 cut(s) 621, 1593, 1650, 2319
SmlI CTYRAG 4 cut(s) 1497, 1563, 1723, 2696
SmoI CTYRAG 4 cut(s) 1497, 1563, 1723, 2696
SnaBI TACGTA 3 cut(s) 208, 1271, 1409
SphI GCATGC 1 cut(s) 1324
SseBI AGGCCT 1 cut(s) 1259
SsiI CCGC 3 cut(s) 2513, 2611, 2613
SspI AATATT 1 cut(s) 2566
SspMI CTAG 2 cut(s) 1809, 2670
StuI AGGCCT 1 cut(s) 1259
StyD4I CCNGG 8 cut(s) 91, 114, 1308, 1572, 1675, 1786, 2327, 2410
StyI CCWWGG 6 cut(s) 590, 1327, 1526, 2234, 2252, 2802
TaaI ACNGT 7 cut(s) 386, 1078, 1165, 1460, 2209, 2594, 2813
TaiI ACGT 9 cut(s) 210, 235, 973, 1213, 1255, 1273, 1411, 2382, 2819
TauI GCSGC 2 cut(s) 2515, 2613
TfiI GAWTC 8 cut(s) 488, 805, 1930, 2119, 2267, 2394, 2424, 2688
TscAI CASTG 5 cut(s) 369, 564, 828, 883, 1083
TseFI GTSAC 2 cut(s) 269, 1080
TseI GCWGC 4 cut(s) 435, 639, 1453, 1772
Tsp45I GTSAC 2 cut(s) 269, 1080
TspGWI ACGGA 2 cut(s) 1412, 1456
TspRI CASTG 5 cut(s) 369, 564, 828, 883, 1083
Tth111I GACNNNGTC 1 cut(s) 1245
Van91I CCANNNNNTGG 1 cut(s) 2770
Vha464I CTTAAG 1 cut(s) 2696
VneI GTGCAC 2 cut(s) 765, 966
VpaK11BI GGWCC 4 cut(s) 386, 683, 1136, 1673
VspI ATTAAT 1 cut(s) 2091
XagI CCTNNNNNAGG 1 cut(s) 1089
XceI RCATGY 4 cut(s) 54, 248, 1324, 1851
XcmI CCANNNNNNNNNTGG 2 cut(s) 777, 1058
XmiI GTMKAC 3 cut(s) 508, 675, 1249
XmnI GAANNNNTTC 1 cut(s) 487
XspI CTAG 2 cut(s) 1809, 2670
Zsp2I ATGCAT 1 cut(s) 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.