Rw2G008860

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000080
Physical Location & Seq
Reverse (-)
8874507 .. 8879348
4842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008860.1

Sequence Viewer

Length: 4188 bp
ATGGAGCGTGTGTGTGCTCGGCACCAACAATGGCGAGAGTCAATGAGTTCTTCACAACGTCAAGCATACTTGGCACGACGACGCGCAATAGCTCAAGAAAAGAATTTCCTCCAAAAAAATGCTAATTCATTTGTGTTAATAGAAGAATTTGACAATGAAGGCACAAGTTCAAGAATTTCTTCAAATTCCAACAACCCCGAACAAGAATACTCAAGGGTTCGTTTAACTCGCATAAGACAAATGGCATGTTCGAATAGATCAGAAACAAGCGATGGAGTTGTCCGAATTTCTACTCGCTATGGTGAGCTTAATATATTAAACTTATTGATAGCGAATCATCTGTATTCTCCCCCAGAAATGGTTCACCTCTTTTCTGATCAAACTGATGAGGGGAGAAAATTCAGACAAAATATTAGGGCCTACAACAATGTGTTTGCATTCACTTCAATGGGCGTACATGTTGATGAAAGAATTAATATCCAAGGCCGTGGACCTTATACGTTTCGTGCACAAGGTTCTATATATCATAAAATTAGTGGTCTATTACCAATTGACGGGAGAAGACCACGATATCTACAGGCTTATATATACGACACTGAGCATGAACTTGAGAATAGATTGTCTGAAAGCGAAGTTTTGGAGAAGGGAATGGTTGAAAAGATACAACAAATTCTTAATCAACACAATCCATTTGTTCAAACATTTCGAAGTCTCGGACAACGTCAGGATTTGCCCAATTGCAGACTCATCATCAGAGAGCAACCTTCCGACCGCCGTCAGTATAATTTGCCATCTGCATCACAGGTTGCTGCTATTATAGTAGGGGGTGACGACATCAATCCAAATGGGAGAGATTTTATTGTTCAAACAATTAGTGGACAACTTTGGAATGTCAAAGACTCAGTTGGATATTATGACCCCATGCAGTATCCGTTATTATTGCCTTATGGAACATATGGATGGGACATTAATACTCATGGCAATAATGGAAGACCAATGTCATGTTGTGACTACTACGCATATATGTTGCAGATGCGTCAAGGTCATGAAAATCCTCTACATCGAGGAGGACGCCTCTTCCAACAGTATGTTAAGCTCAGATGGCTTCGTAGTAATCAAGAAAAATTTCGGAGAGAATTTCGTCAAGGACTTCAAGACTCTCTACATGCAGGAGAAAATGATGCAGGTAACGTTGGTCGTAGAACAATTCTGCCATCATCGTTTATTGGAAGCCCACGTGACATGTACCAACGATATCAAGATGCAATGGCTTTGGTTCAAAAATATGGCAGGCCGGATCTTTTTATCACCATGACCTGTAACCCCAATTGGGAAGAAGTTAGAAATGAACTACTCCCTGGACAAACGCCACAAGACCGTCCTGATTTAGTCACCAGGGTATTTCATGCCAAGTTTGAGCAATTAAAAAAAGACATCATCAACAAGGGGGTGCTGGGAAAAGTGGCTGCACATGCTTTCGTTGTTGAATTTAAAAAACGGGGCCTACCACATGGTCATATGCTGATTATGTTAGAAGAAAATGACAAGCTTAATAACCCTGATGAATATGATCTCATTGTTAGAGCTGAAATACCATATGAGGACGAGGAGCCTCAACTTTATGATGCAGTGTGTACACATATGATTCATGGTCCATGTGGAACTCTTAACCCAAGGCAGTCATGTATGAAAAATGGAAGTTGCAATAAAGGCTACCCAAAACCATTTGCAAATTTCACTGTGCAAGGAAACGATGCATATCCTGTATATCGGAGACGAGCAAGTCGTTTACCAGTACCTTTGCGTAGACGAGGAGATGTGATGGTGGATAACGGTTGGGTGGTTCCCTACAACCCATGGTTACTATTACGGTATAATTGTCACATCAATGTTGAAATATGTGGGAGCATAAAAAGTGTCAAGTACTTATATAAGTATATCTACAAAGGTCCTGACAGAGTGGCACTTGAGCTGCAATCAAACCCAGAGTTTGATGAAATTAGACAATTTGTTGATGCAAGGTGGGTCTGTGCACCAGAGGCTTTATGGAGGATCTTCAAATTTGCAATGAACAGAATATATCCAACAGTAGAGCGATTACAAATACATCTTCCCAACATGCAACAGATAACATTCAATGTTGATGAAACAGTAGATAATATTCTGGCAGATGAGCACACACAAATGTCAATGCTTACTGAGTTTTTCACCATAAATCGAATGGATGAGGATGCAAGAGCATATTTGTATCGAGAAATTCCTGAACATTACAGATGGGATAATAGCAACAAAATTTGGGTGAAAAGAAGAAGAAACTACAAGGTTATTGGGCGAATATACAGCGTCTCACCGTTTGAAGGTGAGAAATTTTACCTGCGTGTCCTTCTTAATCATGTAAGAGGTCCAAGATCATTTCGTGATTTGCTTACTATTAATGGTGTTCTACAACCAACTTTCAAGCAAGCAGCAGAGAAACAAGGTTTGCTAGAAAATGATAATAGTATCCGACAGTGTTTGTTGGAGGCCTCTGCAATTAGGATGCCATCAACTTTGAGGAGATTATTCGTCACAATTTTAGTGTACAATGAGCCAATTGGTGTCCGAACATTGTGGGATGAATTTGTCCCTTTTATGGTGGAAGATTATCCTTCATCTGGTGATATTGCAAACCGAAACAGACTCTTACAAGATTTAAATGGAGTACTTAAACAATTTAACAGGAACATTAGAGATTTTGACCTACCTGAAATGACAACAGAGCCAGAAGAAAATTCAAGAATGCCAAGGTGTATTGAAGACGAACTTTCCATACATATCTCTCAAGAAGACATTGATGCAATTGCCCACCTTAATGATGACCAAAGGATCGCCTTTAACACGATCATGGATGCAGTTCAGAGAAAAAGAAGTGCATTATTTTTTGTTGATGGTCCTAGTGGAACTGGGAAGACATATTTATACCGTGCATTGCTAGCAAACATACGAAGAATGAATCATATAGTCTTAGCCACAACATCATCTGGAATAGCTGCAAATATACTGCCCGGCGGGAGGATAGCTCACTCCAGATTTAAGATTCCACTCAATGTAGATGCGTCATCTATGTGTTCCATAAGTAAACAGTCTGCTCTGGCACAATTGGTACAGGATTCGACTGCAATTATTTGGGATGAGGCACCTATGACACATCGACATGTCTTTGAAGCACTTGATCGAACTTTTAGAGACATAATGGATGTCGACTTACCATTTGGTGGAAAGATAATGATTTTTGGGGGAGATTTTCGTCAAGTCCTCCCTGTTATTCCTAAAGGAACGAAGTCTGAACTCATTCAAGCCTCCATTGTGAAATCAACATTTTGGACACAGATAAAGATATTAAGACTGAGACAGAATATGAGATCCATAAATGACCACCAATTTGCAGAGTTTCTTCTTCGTGTAGGCGATGGGAGTGAACAAGTCATCAAAGACGAAATGATAAGATTACCGGAATGCATGGTGGTTCCATGGGAAAGCGACCAATCTATCAATCTACTTATTGATGAAGTTTTCCCCAATTTGGGTGATCATGTAAATGATGCAAGATACATGGTGGACAGAGCATTGATTACTCCTATAAACGACGATGTGGACGTGTTGAATGAAAAGATAATCCATATGTTTCCAGGTGAAGAAATTACATTGTACTCATTTGACTCTGTTGAAGATGACATCAGAAATTTGTACCAACCAGAGTTTCTGAACTCAATAACCGCAGGTGGGCTGCCACCCCACAAGTTAACTCTGAAAGTAGGTGCTCCAATCATGCTTTTGAGGAATATTGACCCTAAATTAGGATTGTGTACTAGATTGTTGTGTCGTGGTTCGTACCAAAATCTGATTGATGCTGAGATACTAACAGGACAATTTACTAGGACTAGAGTTTTTCTACCTAGAATTCCTTTGAAAAGTGCAGAAAATGTTGGGCTCCCATTTCAACTAACAAGAAAGCAATTTCCAGTGAAACTGAGCTTCTGTTTGACCATAAACAAATCTCAAGGCCAGACTATCCCACATGTAGGTGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTATATGTGGCATTGTCGAGAGGAGTGAATGGTACCAGATCGTCGCCCTCAACAAAAGAAAAAGTTCTAAAGAGACAAGGGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1395

Amino Acids

160.89

Weight (kDa)

8.62

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 338 - 509 6.3e-56 Helitron helicase-like domain at N-terminus
PIF1 PF05970 950 - 1154 2e-75 PIF1-like helicase
Pif1_2B_dom PF21530 1246 - 1282 9.1e-16 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3749
AasI GACNNNNNNGTC 1 cut(s) 1782
Acc36I ACCTGC 3 cut(s) 1175, 2382, 3749
Acc65I GGTACC 1 cut(s) 4133
AccB1I GGYRCC 3 cut(s) 21, 3175, 4133
AccB7I CCANNNNNTGG 1 cut(s) 3254
AccI GTMKAC 2 cut(s) 1807, 3240
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 772, 3048, 3756
AclI AACGTT 1 cut(s) 1191
AclWI GGATC 4 cut(s) 1305, 2060, 2873, 3396
AcvI CACGTG 1 cut(s) 1238
AcyI GRCGYC 1 cut(s) 1072
AflIII ACRYGT 5 cut(s) 457, 1242, 3193, 3636, 4057
AjiI CACGTC 1 cut(s) 3637
AjnI CCWGG 3 cut(s) 1357, 1394, 3667
AjuI GAANNNNNNNTTGG 2 cut(s) 3723, 3755
AluBI AGCT 9 cut(s) 92, 307, 1096, 1549, 1586, 1972, 3029, 3059, 4014
AluI AGCT 9 cut(s) 92, 307, 1096, 1549, 1586, 1972, 3029, 3059, 4014
Alw21I GWGCWC 5 cut(s) 19, 511, 2035, 2178, 3802
Alw26I GTCTC 6 cut(s) 716, 1768, 2350, 3219, 3382, 4168
Alw44I GTGCAC 2 cut(s) 507, 2031
AlwI GGATC 4 cut(s) 1305, 2060, 2873, 3396
AoxI GGCC 7 cut(s) 417, 484, 1292, 1501, 2523, 4042, 4181
ApaLI GTGCAC 2 cut(s) 507, 2031
ApeKI GCWGC 6 cut(s) 809, 1466, 1972, 2465, 3029, 3766
ArsI GACNNNNNNTTYG 4 cut(s) 3233, 3265, 3404, 3436
AseI ATTAAT 3 cut(s) 474, 969, 2433
Asp700I GAANNNNTTC 5 cut(s) 178, 360, 1125, 3330, 4164
Asp718I GGTACC 1 cut(s) 4133
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 8 cut(s) 417, 491, 1501, 1652, 1949, 2402, 2930, 4181
AsuC2I CCSGG 1 cut(s) 3045
AsuII TTCGAA 2 cut(s) 251, 706
AsuNHI GCTAGC 1 cut(s) 2971
AvaII GGWCC 5 cut(s) 491, 1652, 1949, 2402, 2930
BaeGI GKGCMC 2 cut(s) 511, 2035
BanI GGYRCC 3 cut(s) 21, 3175, 4133
BanII GRGCYC 1 cut(s) 3972
BbrPI CACGTG 1 cut(s) 1238
BbsI GAAGAC 5 cut(s) 568, 997, 2802, 2832, 2954
Bbv12I GWGCWC 5 cut(s) 19, 511, 2035, 2178, 3802
BbvI GCAGC 6 cut(s) 796, 1453, 1959, 2477, 3016, 3753
BceAI ACGGC 2 cut(s) 471, 759
BciT130I CCWGG 3 cut(s) 1359, 1396, 3669
BciVI GTATCC 2 cut(s) 939, 2513
BclI TGATCA 2 cut(s) 376, 3568
BcnI CCSGG 1 cut(s) 3045
BcoDI GTCTC 6 cut(s) 716, 1768, 2350, 3219, 3382, 4168
BfaI CTAG 7 cut(s) 2486, 2934, 2972, 3849, 3915, 3921, 3936
BfmI CTRYAG 1 cut(s) 575
BfuAI ACCTGC 3 cut(s) 1175, 2382, 3749
BfuI GTATCC 2 cut(s) 939, 2513
BisI GCNGC 6 cut(s) 810, 1467, 1973, 2466, 3030, 3767
BlsI GCNGC 6 cut(s) 811, 1468, 1974, 2467, 3031, 3768
BmcAI AGTACT 2 cut(s) 1925, 2703
Bme1390I CCNGG 4 cut(s) 1359, 1396, 3045, 3669
Bme18I GGWCC 5 cut(s) 491, 1652, 1949, 2402, 2930
BmgBI CACGTC 1 cut(s) 3637
BmgT120I GGNCC 8 cut(s) 417, 491, 1501, 1652, 1949, 2402, 2930, 4181
BmiI GGNNCC 8 cut(s) 23, 1502, 1611, 1845, 3177, 3507, 3971, 4135
BmrFI CCNGG 4 cut(s) 1359, 1396, 3045, 3669
BmrI ACTGGG 1 cut(s) 2952
BmtI GCTAGC 1 cut(s) 2975
BmuI ACTGGG 1 cut(s) 2952
BoxI GACNNNNGTC 2 cut(s) 774, 997
BpiI GAAGAC 5 cut(s) 568, 997, 2802, 2832, 2954
BplI GAGNNNNNCTC 4 cut(s) 1059, 1091, 3043, 3075
BpmI CTGGAG 1 cut(s) 3049
Bpu14I TTCGAA 2 cut(s) 251, 706
BpuEI CTTGAG 6 cut(s) 78, 196, 629, 1988, 2805, 4023
BpuMI CCSGG 1 cut(s) 3045
BsaAI YACGTR 1 cut(s) 1238
BsaBI GATNNNNATC 1 cut(s) 1758
BsaHI GRCGYC 1 cut(s) 1072
BsaJI CCNNGG 8 cut(s) 481, 487, 1357, 1395, 1673, 1856, 2783, 3509
BsaWI WCCGGW 1 cut(s) 3490
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 3 cut(s) 1793, 2947, 4001
Bse3DI GCAATG 3 cut(s) 1272, 2073, 2966
Bse8I GATNNNNATC 1 cut(s) 1758
BseBI CCWGG 3 cut(s) 1359, 1396, 3669
BseDI CCNNGG 8 cut(s) 481, 487, 1357, 1395, 1673, 1856, 2783, 3509
BseGI GGATG 8 cut(s) 965, 2230, 2236, 2544, 2620, 2893, 3175, 3241
BseJI GATNNNNATC 1 cut(s) 1758
BseMI GCAATG 3 cut(s) 1272, 2073, 2966
BseMII CTCAG 7 cut(s) 588, 915, 1111, 2190, 3377, 3882, 4001
BseNI ACTGG 3 cut(s) 1793, 2947, 4001
BseRI GAGGAG 5 cut(s) 1080, 1622, 1827, 2569, 4137
BseSI GKGCMC 2 cut(s) 511, 2035
BseXI GCAGC 6 cut(s) 796, 1453, 1959, 2477, 3016, 3753
BseYI CCCAGC 1 cut(s) 1453
BsgI GTGCAG 2 cut(s) 1452, 3975
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 772
BshFI GGCC 7 cut(s) 419, 486, 1294, 1503, 2525, 4044, 4183
BshNI GGYRCC 3 cut(s) 21, 3175, 4133
BsiEI CGRYCG 1 cut(s) 772
BsiHKAI GWGCWC 5 cut(s) 19, 511, 2035, 2178, 3802
BsiSI CCGG 3 cut(s) 1295, 3045, 3491
BslFI GGGAC 2 cut(s) 977, 2609
BsmAI GTCTC 6 cut(s) 716, 1768, 2350, 3219, 3382, 4168
BsmBI CGTCTC 2 cut(s) 1768, 2350
BsmFI GGGAC 2 cut(s) 977, 2609
BsmI GAATGC 3 cut(s) 437, 2784, 3500
BsnI GGCC 7 cut(s) 419, 486, 1294, 1503, 2525, 4044, 4183
Bsp119I TTCGAA 2 cut(s) 251, 706
Bsp1286I GDGCHC 6 cut(s) 19, 511, 2035, 2178, 3802, 3972
Bsp1407I TGTACA 2 cut(s) 1634, 2580
Bsp19I CCATGG 2 cut(s) 1856, 3509
BspACI CCGC 3 cut(s) 772, 3048, 3756
BspANI GGCC 7 cut(s) 419, 486, 1294, 1503, 2525, 4044, 4183
BspCNI CTCAG 7 cut(s) 589, 914, 1110, 2191, 3378, 3883, 4002
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 8 cut(s) 23, 1502, 1611, 1845, 3177, 3507, 3971, 4135
BspMI ACCTGC 3 cut(s) 1175, 2382, 3749
BspOI GCTAGC 1 cut(s) 2975
BspPI GGATC 4 cut(s) 1305, 2060, 2873, 3396
BspT104I TTCGAA 2 cut(s) 251, 706
BspT107I GGYRCC 3 cut(s) 21, 3175, 4133
BsrDI GCAATG 3 cut(s) 1272, 2073, 2966
BsrGI TGTACA 2 cut(s) 1634, 2580
BsrI ACTGG 3 cut(s) 1793, 2947, 4001
BssECI CCNNGG 8 cut(s) 481, 487, 1357, 1395, 1673, 1856, 2783, 3509
BssNI GRCGYC 1 cut(s) 1072
BssT1I CCWWGG 5 cut(s) 481, 1673, 1856, 2783, 3509
Bst2UI CCWGG 3 cut(s) 1359, 1396, 3669
Bst6I CTCTTC 1 cut(s) 1082
BstACI GRCGYC 1 cut(s) 1072
BstAUI TGTACA 2 cut(s) 1634, 2580
BstBAI YACGTR 1 cut(s) 1238
BstBI TTCGAA 2 cut(s) 251, 706
BstC8I GCNNGC 3 cut(s) 1292, 2463, 2973
BstDEI CTNAG 8 cut(s) 597, 901, 1097, 2199, 3004, 3386, 3891, 4010
BstDSI CCRYGG 3 cut(s) 487, 1856, 3509
BstENI CCTNNNNNAGG 1 cut(s) 3834
BstF5I GGATG 8 cut(s) 965, 2230, 2236, 2544, 2620, 2893, 3175, 3241
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 86
BstMAI GTCTC 6 cut(s) 716, 1768, 2350, 3219, 3382, 4168
BstMCI CGRYCG 1 cut(s) 772
BstMWI GCNNNNNNNGC 6 cut(s) 71, 1102, 1472, 1710, 2039, 2972
BstNI CCWGG 3 cut(s) 1359, 1396, 3669
BstNSI RCATGY 8 cut(s) 249, 461, 1169, 1246, 1475, 2122, 3197, 4061
BstPAI GACNNNNGTC 2 cut(s) 774, 997
BstSCI CCNGG 4 cut(s) 1357, 1394, 3043, 3667
BstSFI CTRYAG 1 cut(s) 575
BstSLI GKGCMC 2 cut(s) 511, 2035
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 6 cut(s) 796, 1453, 1959, 2477, 3016, 3753
BstV2I GAAGAC 5 cut(s) 568, 997, 2802, 2832, 2954
BstX2I RGATCY 3 cut(s) 1297, 2052, 3401
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 3 cut(s) 1297, 2052, 3401
BsuI GTATCC 2 cut(s) 939, 2513
BsuRI GGCC 7 cut(s) 419, 486, 1294, 1503, 2525, 4044, 4183
BtgI CCRYGG 3 cut(s) 487, 1856, 3509
BtgZI GCGATG 2 cut(s) 285, 3462
BtrI CACGTC 1 cut(s) 3637
BtsCI GGATG 8 cut(s) 965, 2230, 2236, 2544, 2620, 2893, 3175, 3241
BtsI GCAGTG 1 cut(s) 1635
BtsIMutI CAGTG 5 cut(s) 594, 1635, 1737, 2516, 4008
BveI ACCTGC 3 cut(s) 1175, 2382, 3749
Cac8I GCNNGC 3 cut(s) 1292, 2463, 2973
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 8 cut(s) 417, 491, 1501, 1652, 1949, 2402, 2930, 4181
CseI GACGC 5 cut(s) 90, 1025, 1080, 2332, 3084
DdeI CTNAG 8 cut(s) 597, 901, 1097, 2199, 3004, 3386, 3891, 4010
DraI TTTAAA 2 cut(s) 1492, 2694
DrdI GACNNNNNNGTC 1 cut(s) 1782
DseDI GACNNNNNNGTC 1 cut(s) 1782
Eam1104I CTCTTC 1 cut(s) 1082
EarI CTCTTC 1 cut(s) 1082
Eco130I CCWWGG 5 cut(s) 481, 1673, 1856, 2783, 3509
Eco147I AGGCCT 1 cut(s) 2525
Eco24I GRGCYC 1 cut(s) 3972
Eco32I GATATC 2 cut(s) 572, 1256
Eco47I GGWCC 5 cut(s) 491, 1652, 1949, 2402, 2930
Eco72I CACGTG 1 cut(s) 1238
EcoNI CCTNNNNNAGG 1 cut(s) 3834
EcoO109I RGGNCCY 3 cut(s) 417, 1501, 1949
EcoRI GAATTC 1 cut(s) 3939
EcoRII CCWGG 3 cut(s) 1357, 1394, 3667
EcoRV GATATC 2 cut(s) 572, 1256
EcoT14I CCWWGG 5 cut(s) 481, 1673, 1856, 2783, 3509
EcoT22I ATGCAT 2 cut(s) 1759, 3500
EcoT38I GRGCYC 1 cut(s) 3972
ErhI CCWWGG 5 cut(s) 481, 1673, 1856, 2783, 3509
Esp3I CGTCTC 2 cut(s) 1768, 2350
FalI AAGNNNNNCTT 4 cut(s) 163, 195, 2787, 2819
FaqI GGGAC 2 cut(s) 977, 2609
FauI CCCGC 1 cut(s) 3041
FauNDI CATATG 5 cut(s) 955, 1518, 1597, 1641, 3660
FbaI TGATCA 2 cut(s) 376, 3568
FblI GTMKAC 2 cut(s) 1807, 3240
Fnu4HI GCNGC 6 cut(s) 810, 1467, 1973, 2466, 3030, 3767
FokI GGATG 8 cut(s) 972, 2237, 2243, 2551, 2627, 2900, 3182, 3248
FriOI GRGCYC 1 cut(s) 3972
Fsp4HI GCNGC 6 cut(s) 810, 1467, 1973, 2466, 3030, 3767
FspBI CTAG 7 cut(s) 2486, 2934, 2972, 3849, 3915, 3921, 3936
GlaI GCGC 1 cut(s) 85
GluI GCNGC 6 cut(s) 810, 1467, 1973, 2466, 3030, 3767
GsaI CCCAGC 1 cut(s) 1457
GsuI CTGGAG 1 cut(s) 3049
HaeIII GGCC 7 cut(s) 419, 486, 1294, 1503, 2525, 4044, 4183
HapII CCGG 3 cut(s) 1295, 3045, 3491
HgaI GACGC 5 cut(s) 90, 1025, 1080, 2332, 3084
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 1072
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 2 cut(s) 3241, 3783
HindII GTYRAC 2 cut(s) 3241, 3783
HindIII AAGCTT 1 cut(s) 1547
HpaI GTTAAC 1 cut(s) 3783
HpaII CCGG 3 cut(s) 1295, 3045, 3491
Hpy99I CGWCG 4 cut(s) 81, 84, 3629, 4147
HpyAV CCTTC 6 cut(s) 152, 637, 774, 2351, 2393, 2658
HpyCH4IV ACGT 6 cut(s) 58, 500, 721, 1191, 1237, 3636
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 1102, 1472, 1710, 2039, 2972
HpyF3I CTNAG 8 cut(s) 597, 901, 1097, 2199, 3004, 3386, 3891, 4010
HpySE526I ACGT 6 cut(s) 58, 500, 721, 1191, 1237, 3636
Hsp92I GRCGYC 1 cut(s) 1072
HspAI GCGC 1 cut(s) 84
KpnI GGTACC 1 cut(s) 4137
Ksp22I TGATCA 2 cut(s) 376, 3568
KspAI GTTAAC 1 cut(s) 3783
LmnI GCTCC 5 cut(s) 4, 1609, 1905, 3805, 3975
Lsp1109I GCAGC 6 cut(s) 796, 1453, 1959, 2477, 3016, 3753
MaeI CTAG 7 cut(s) 2486, 2934, 2972, 3849, 3915, 3921, 3936
MaeII ACGT 6 cut(s) 58, 500, 721, 1191, 1237, 3636
MaeIII GTNAC 9 cut(s) 827, 1007, 1187, 1238, 1319, 1390, 1860, 1880, 2566
MfeI CAATTG 6 cut(s) 549, 736, 1327, 2592, 2838, 3137
MflI RGATCY 3 cut(s) 1297, 2052, 3401
MhlI GDGCHC 6 cut(s) 19, 511, 2035, 2178, 3802, 3972
MlyI GAGTC 6 cut(s) 47, 738, 893, 1151, 2673, 3692
MmeI TCCRAC 7 cut(s) 213, 792, 886, 1105, 2108, 2499, 2530
Mph1103I ATGCAT 2 cut(s) 1759, 3500
MroXI GAANNNNTTC 5 cut(s) 178, 360, 1125, 3330, 4164
MspI CCGG 3 cut(s) 1295, 3045, 3491
MspR9I CCNGG 4 cut(s) 1359, 1396, 3045, 3669
MunI CAATTG 6 cut(s) 549, 736, 1327, 2592, 2838, 3137
Mva1269I GAATGC 3 cut(s) 437, 2784, 3500
MvaI CCWGG 3 cut(s) 1359, 1396, 3669
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 6 cut(s) 71, 1102, 1472, 1710, 2039, 2972
NciI CCSGG 1 cut(s) 3045
NcoI CCATGG 2 cut(s) 1856, 3509
NdeI CATATG 5 cut(s) 955, 1518, 1597, 1641, 3660
NheI GCTAGC 1 cut(s) 2971
NlaIV GGNNCC 8 cut(s) 23, 1502, 1611, 1845, 3177, 3507, 3971, 4135
NmuCI GTSAC 6 cut(s) 827, 1007, 1238, 1390, 1880, 2566
NsiI ATGCAT 2 cut(s) 1759, 3500
NspI RCATGY 8 cut(s) 249, 461, 1169, 1246, 1475, 2122, 3197, 4061
NspV TTCGAA 2 cut(s) 251, 706
PagI TCATGA 1 cut(s) 1045
PaqCI CACCTGC 1 cut(s) 3749
PceI AGGCCT 1 cut(s) 2525
PciI ACATGT 4 cut(s) 457, 1242, 3193, 4057
PcsI WCGNNNNNNNCGW 2 cut(s) 1783, 3633
PctI GAATGC 3 cut(s) 437, 2784, 3500
PdmI GAANNNNTTC 5 cut(s) 178, 360, 1125, 3330, 4164
PfeI GAWTC 5 cut(s) 334, 1645, 2992, 3076, 3149
PflFI GACNNNGTC 1 cut(s) 720
PflMI CCANNNNNTGG 1 cut(s) 3254
PkrI GCNGC 6 cut(s) 811, 1468, 1974, 2467, 3031, 3768
PleI GAGTC 6 cut(s) 46, 738, 893, 1151, 2673, 3692
PmaCI CACGTG 1 cut(s) 1238
PmlI CACGTG 1 cut(s) 1238
PpsI GAGTC 6 cut(s) 46, 738, 893, 1151, 2673, 3692
Ppu21I YACGTR 1 cut(s) 1238
PpuMI RGGWCCY 1 cut(s) 1949
PscI ACATGT 4 cut(s) 457, 1242, 3193, 4057
PshAI GACNNNNGTC 2 cut(s) 774, 997
PshBI ATTAAT 3 cut(s) 474, 969, 2433
Psp1406I AACGTT 1 cut(s) 1191
Psp5II RGGWCCY 1 cut(s) 1949
Psp6I CCWGG 3 cut(s) 1357, 1394, 3667
PspCI CACGTG 1 cut(s) 1238
PspFI CCCAGC 1 cut(s) 1453
PspGI CCWGG 3 cut(s) 1357, 1394, 3667
PspN4I GGNNCC 8 cut(s) 23, 1502, 1611, 1845, 3177, 3507, 3971, 4135
PspPI GGNCC 8 cut(s) 417, 491, 1501, 1652, 1949, 2402, 2930, 4181
PspPPI RGGWCCY 1 cut(s) 1949
PsrI GAACNNNNNNTAC 2 cut(s) 2793, 2825
PsuI RGATCY 3 cut(s) 1297, 2052, 3401
PsyI GACNNNGTC 1 cut(s) 720
SalI GTCGAC 1 cut(s) 3239
SatI GCNGC 6 cut(s) 810, 1467, 1973, 2466, 3030, 3767
Sau96I GGNCC 8 cut(s) 417, 491, 1501, 1652, 1949, 2402, 2930, 4181
ScaI AGTACT 2 cut(s) 1925, 2703
SchI GAGTC 6 cut(s) 47, 738, 893, 1151, 2673, 3692
ScrFI CCNGG 4 cut(s) 1359, 1396, 3045, 3669
SduI GDGCHC 6 cut(s) 19, 511, 2035, 2178, 3802, 3972
SfcI CTRYAG 1 cut(s) 575
SfuI TTCGAA 2 cut(s) 251, 706
SinI GGWCC 5 cut(s) 491, 1652, 1949, 2402, 2930
SmiI ATTTAAAT 1 cut(s) 2694
SmlI CTYRAG 6 cut(s) 93, 211, 608, 1967, 2820, 4038
SmoI CTYRAG 6 cut(s) 93, 211, 608, 1967, 2820, 4038
SseBI AGGCCT 1 cut(s) 2525
SsiI CCGC 3 cut(s) 772, 3048, 3756
SspI AATATT 3 cut(s) 412, 2161, 3823
SspMI CTAG 7 cut(s) 2486, 2934, 2972, 3849, 3915, 3921, 3936
StuI AGGCCT 1 cut(s) 2525
StyD4I CCNGG 4 cut(s) 1357, 1394, 3043, 3667
StyI CCWWGG 5 cut(s) 481, 1673, 1856, 2783, 3509
SwaI ATTTAAAT 1 cut(s) 2694
TaiI ACGT 6 cut(s) 61, 503, 724, 1194, 1240, 3639
TatI WGTACW 6 cut(s) 1634, 1923, 2580, 2701, 3687, 3845
TfiI GAWTC 5 cut(s) 334, 1645, 2992, 3076, 3149
TscAI CASTG 5 cut(s) 601, 1635, 1744, 2516, 4008
TseFI GTSAC 6 cut(s) 827, 1007, 1238, 1390, 1880, 2566
TseI GCWGC 6 cut(s) 809, 1466, 1972, 2465, 3029, 3766
Tsp45I GTSAC 6 cut(s) 827, 1007, 1238, 1390, 1880, 2566
TspGWI ACGGA 1 cut(s) 921
TspRI CASTG 5 cut(s) 601, 1635, 1744, 2516, 4008
Tth111I GACNNNGTC 1 cut(s) 720
Van91I CCANNNNNTGG 1 cut(s) 3254
VneI GTGCAC 2 cut(s) 507, 2031
VpaK11BI GGWCC 5 cut(s) 491, 1652, 1949, 2402, 2930
VspI ATTAAT 3 cut(s) 474, 969, 2433
XagI CCTNNNNNAGG 1 cut(s) 3834
XceI RCATGY 8 cut(s) 249, 461, 1169, 1246, 1475, 2122, 3197, 4061
XcmI CCANNNNNNNNNTGG 2 cut(s) 2043, 2218
XmiI GTMKAC 2 cut(s) 1807, 3240
XmnI GAANNNNTTC 5 cut(s) 178, 360, 1125, 3330, 4164
XspI CTAG 7 cut(s) 2486, 2934, 2972, 3849, 3915, 3921, 3936
ZrmI AGTACT 2 cut(s) 1925, 2703
Zsp2I ATGCAT 2 cut(s) 1759, 3500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.