Rw0G022900

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01062
OG0000080
Physical Location & Seq
Reverse (-)
13132 .. 19110
5979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022900.1

Sequence Viewer

Length: 4074 bp
ATGCCTAATGTCACAATGAATAATCACGATGAGGACCACAATAACAACGAGCATATGGTGAATAATCATGAGGATCAGAACAACAACACTGAGCTAGAGAATAACCCACAGGATCAGCGCAGCAGCAGTGTTGACGCAGTGCGGCGTACAAGGAGAGCAGTAGTAAATTACAACTGCGCTAGAGATTTTAATGAGGGATGGGGTGTCCCATTTTGTCGATTGTCTCCACTAAGAACATGTCCATACTGCAATGCACGGTTATTTCATCGAGAGACCTTCAATTTGTGTTGTTCGAAAGGAAATATTGTTCTGCCTCTAATACCCTCTCCGCCAGAGATGATCCAGCTCTTTTCTGACCAAACAGATGTGGGTAGAAAATTCAGACAAAATATCCGAGCCTATAATAATGTGTTTGCGTTCACTTCAATGGGTGTGCATGTTGATGAATCCATTAATCTTCAAGGTCGTGGAATTTATACTTTTCGTGCACAAGGTTCTATATATCATAAGATTGGTGGGCTTTTACCAAGTGAAGGGGCGAGACCACGATATCTACAAGCTTACATATATGACACCGAGCATGAACTTGACAATCGATTGTCTGAAAGTGTAGTTTTAGACAGGGCACTGGTTGAAAAGATACAACAGATATTGAATCACTATAATCCATTTGTCCATCAATTTCGAAGCCTCGGACAGCGCCAAGACTTGCCCAATTGCAGGCTCATCATAAGAGAGCAGCCTTCTAATCAACGTCAGTATTGTTTACCATCTGCATCACAAGTTGCTGCTATTATAGTAGGAGGGGGCGACATCAATCCGAATGGAAGAGATTTAATTGTCCAAACAATTAGTGGAAGACTCTGGAATGTTAAAGACTCGGTCGGATATTACGACCCCATGCAGTATCCTTTGTTATTACCTTATGGAACATATGGGTGGGATATTAATAGTCATGATGAGAATGGAAGACCTATGTCATGTTGCGACTATTATGCATATATGTTACAGATGCGCCCAGGTGATGAAAATCCTCTACTTCGAGGCGGACGGCTCTTACAACAGTATGTTGTAGATAATTACGTGAAAATTGAATCCCAAAAACTCAGATGGCTTCGCAGTAACCAAGATACAATTAGGAAAGAATTTTATCAAGGACTTCAAGACTCTTTTATTGCAGGGGAAAACAATGCAGGTAACATCGGTCATAGGACAATTCTGCCATCATCATTTGTTGGAAGTCCACGTGATATGTACCAACGGTACCAAGATGCGATGGCTTTGGTTCAAAAGTATGGCAGACCAGATTTGTTTATCACTATGACGTGTAACCCGAATTGGGAAGAAATTAAAAGTGAGCTACTCCCTGGACAAACATCACAAGATCGTCCAGATTTAGAGGTCAGGGTATTTCGTTCAAAACTTGAGCAGCTAAAAGAAGACATCATCACCAAAGGGGTATTGGGAAGAGTTATTGCTCATGCTTTTGTCGTTGAATTTCAGAAACGGGGCCTACCACATGTACACATGCTGGTCATGTTGGATGAAAATGATAAGCTTAATAACCCTGAAGATTATGACCGCATTGTTAGAGCAGAAATACCAGATAAAGATGAAGAGCCTCAACTCTATGATCTGGTGTTGAAACATATGATTCATGGTCCATGTGGAACCTTAAACCAATATTATCCAAAATCATTTGCAAATTTCACTGTGCAAGGAAACGATGCATATCCTATATATCGAAGACGAGCAAGTCGCTTACCAGTCCCTCTACGTAGATCTGGAGACACAATGGTGGATAATAGTTGGGTTGTTCCTTATAACCCGTGGTTGTTGCTACGGTATGATTGTCACATCAATGTTGAAATATGTGGGAGCATAAAGAGTGTCAAATACTTATATAAGTATGTCTACAAAGGTCCTGATAGAGTTGCAGTTCAAGTGCAATCTGACCCGGAGTTCGATGAAATAAGACAATTTGTCGATGCAAGGTGGGTTTGTGCACCAGAGGCTTTATGGAGGATATTTAAATTTGTAATGAACAGAATATATCCAACAGTCGACAGATTACAAATACATCTTCCAAACATGCAACAAATCAGATTCGATGCAAATGAGACTGTAGAAGACATTCTAGCAGATGAGCATGCACAGAAGTCTATGCTTACTGAATATTTCACCATGAACCGTATGGATCCGGAAGCAAGACGATATCTGTATCGAGAATTCCCTCAATACTATAGGTGGGATAGTAAGCAAAAAGCTTGGGTGAAAAGAAGGAACAACTACAAGGTAATTGGAAGAATATACAGTGTTTCACCCTCTGAAGGTGAGAAATTTTACCTACGTGTCCTTCTCAATCATATAAGAGGACCGAGATCATTTCGTGATTTGCTTACTATTAATGGAGTTCTGCAAACTACTTTTAAGGAAGCGGCAGAGAAACGAGAGGCCTCAACAATTAGAATGCCATCAGCTTTGAGAAGATTATTCATCACAATTTTGGTGTATAGTCAACCAACTGGTGTTCGAAGATTGTGGGACGAATTTTATCCTTTCATGATTGAAGATTATTCTTCTTCAAGTGATATTGTCGATATTTCTGTTAGAAACAGGCTACTTCGAGATCTAAACAGAATGTTTGAACAATTTAATAAGAGCACTAGAGAATTTGATCTGCCCGAAATGACAAGTGAACCAGAAGATAGTCTGGAAATAACGAGATGTATTGAAGATGAAATTTCTATAAATATCCCACAAGAAGATATTGATGCAATTGACCACCTTAACAACGACCAAATGATTGCATTCAACACCATAATGGCCGCAGTTGAGGGAAGAGAAAGTGCATTATTTTTTATCGATGGTCCTGGAGGTACTGGGAAGACGTACTTATATCGTGCATTGCTAGCAAAGTTGAGAAGAATGAATCATATAGTCTTAGCCACAGCATTATCTGGAATTGCTGCAAATTTATTGCCCGGCGGGAGGACAGCTCACTCTAGATTCAAGATTCCACTCAATGTAGATGCATCATCCATGTGTTCCATAAGTAAACAATCTGCTCTAGCACGACTAATACAAGATTCCACTGCAATTATTTGGGATGAGGCACCTATGACACATCGACATGTGTTTGAAGCACTTGATCGAACATTTCGAGACATAATGGATGTCGAATTACCGTTTGGTGGAAAGATAATGATTTTTGGGGGAGATTTTCGTCAAGTTCTCCCTGTTATTCCTAAAGGAACGAAGTCTGAACTCATTCAAGCCTCCATTGTGAAAGCAACATTTTGGGCACAAACAAAGATATTGAGATTGAGACAGAATATGAGATCCATAAATGACCACCAATTTGCAAAATTTCTACTTCGTGTTGGCGATGGGAGTGAACAAGTCATTGAAGACGAAATGATGAAATGTGACCAATCTATCAATCAAATTATTGATGAAGTTTTCCCCAATTTGGGTGATCATGTAAATGATGCAAGATACATGGTGGACAGAGCATTGATTACTCCTATAAACGATGATGTGGACGTGTTGAATGAAAAAATAATCAATATGTTTCCGGGTGAAGAAATTACATTGTACTCATTTGACTCTGTTGAAGATGACATGAGAAATTTGTACCAACCAGAGTTTCTGAACTCAATAACCGCAGGTGGGCTGCCACCCCACAAGTTAACTTTGAAAGTAGGTGCTCCAATCATGCTTTTAAGGAATATTGACCCTAAATTGGGATTGTGTAACGGTACTAGATTGTTGTGTCGTGGTTCGTACCAAAATCTGATTGATGCTGAGATACTAACAGGACAGTTTGCTGGGACTAGAGTTTTTCTACCTAGAATTCCTTTGAAAAGTGCAGAAAATGCTGGGCTACCATTTCAACTAACAAGAAAGCAATTTCCAGTCAAACTGAGCTTCTCTTTGACCATAAACAAATCTCAAGGTCAGACTATCCCACATGTAGGTGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTATATGTTGCATTGTCGAGAGGAGCCCGTGGGGCTGGCGGCCTGTCTGGCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1357

Amino Acids

156.11

Weight (kDa)

6.72

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 331 - 512 4.5e-63 Helitron helicase-like domain at N-terminus
PIF1 PF05970 930 - 1134 3.4e-73 PIF1-like helicase
Pif1_2B_dom PF21530 1216 - 1258 1.1e-20 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1824
AarI CACCTGC 1 cut(s) 3659
AasI GACNNNNNNGTC 1 cut(s) 1755
Acc36I ACCTGC 2 cut(s) 1184, 3659
Acc65I GGTACC 1 cut(s) 1263
AccB1I GGYRCC 2 cut(s) 1263, 3115
AccI GTMKAC 2 cut(s) 1914, 2064
AccIII TCCGGA 1 cut(s) 2200
AciI CCGC 9 cut(s) 142, 329, 1047, 1582, 2438, 2829, 2988, 3666, 4056
AclWI GGATC 6 cut(s) 81, 120, 334, 2192, 2205, 3336
AcoI YGGCCR 1 cut(s) 2826
AcuI CTGAAG 2 cut(s) 1590, 2349
AcvI CACGTG 1 cut(s) 1247
AflIII ACRYGT 7 cut(s) 236, 1325, 1519, 2350, 3133, 3546, 3973
AhdI GACNNNNNGTC 1 cut(s) 1982
AjiI CACGTC 2 cut(s) 1326, 3547
AjnI CCWGG 3 cut(s) 1018, 1366, 2872
AjuI GAANNNNNNNTTGG 4 cut(s) 3174, 3206, 3633, 3665
AleI CACNNNNGTG 1 cut(s) 1796
AloI GAACNNNNNNTCC 2 cut(s) 291, 323
Alw21I GWGCWC 4 cut(s) 490, 2008, 2666, 3712
Alw26I GTCTC 7 cut(s) 228, 266, 535, 1782, 2114, 3159, 3322
Alw44I GTGCAC 2 cut(s) 486, 2004
AlwI GGATC 6 cut(s) 81, 120, 334, 2192, 2205, 3336
Aor13HI TCCGGA 1 cut(s) 2200
AoxI GGCC 4 cut(s) 1510, 2454, 2826, 4057
ApaLI GTGCAC 2 cut(s) 486, 2004
ApeKI GCWGC 7 cut(s) 120, 123, 739, 788, 1429, 2969, 3676
ArsI GACNNNNNNTTYG 6 cut(s) 265, 297, 1946, 1978, 3344, 3376
AseI ATTAAT 3 cut(s) 453, 948, 2406
Asp700I GAANNNNTTC 2 cut(s) 2133, 3270
Asp718I GGTACC 1 cut(s) 1263
AspLEI GCGC 4 cut(s) 120, 179, 702, 1017
AspS9I GGNCC 6 cut(s) 34, 1510, 1661, 1922, 2375, 2870
AsuC2I CCSGG 3 cut(s) 1958, 2985, 3579
AsuHPI GGTGA 9 cut(s) 70, 1034, 1441, 2173, 2284, 2313, 2345, 3488, 3593
AsuII TTCGAA 3 cut(s) 293, 685, 2533
AsuNHI GCTAGC 1 cut(s) 2911
AvaII GGWCC 5 cut(s) 34, 1661, 1922, 2375, 2870
BaeGI GKGCMC 4 cut(s) 490, 628, 2008, 3307
BamHI GGATCC 1 cut(s) 2197
BanI GGYRCC 2 cut(s) 1263, 3115
BanII GRGCYC 1 cut(s) 4045
BbrPI CACGTG 1 cut(s) 1247
BbsI GAAGAC 7 cut(s) 865, 976, 1446, 1753, 2136, 2894, 3417
Bbv12I GWGCWC 4 cut(s) 490, 2008, 2666, 3712
BbvI GCAGC 7 cut(s) 132, 135, 751, 775, 1441, 2956, 3663
BccI CCATC 9 cut(s) 192, 684, 778, 1104, 1231, 1270, 2482, 2861, 3383
BceAI ACGGC 1 cut(s) 1067
BcgI CGANNNNNNTGC 2 cut(s) 977, 1011
BciT130I CCWGG 3 cut(s) 1020, 1368, 2874
BciVI GTATCC 1 cut(s) 918
BclI TGATCA 1 cut(s) 3478
BcnI CCSGG 3 cut(s) 1958, 2985, 3579
BcoDI GTCTC 7 cut(s) 228, 266, 535, 1782, 2114, 3159, 3322
BfmI CTRYAG 2 cut(s) 2124, 2242
BfoI RGCGCY 1 cut(s) 703
BfuAI ACCTGC 2 cut(s) 1184, 3659
BfuI GTATCC 1 cut(s) 918
BglI GCCNNNNNGGC 2 cut(s) 4049, 4065
BglII AGATCT 2 cut(s) 1781, 2629
Bme1390I CCNGG 6 cut(s) 1020, 1368, 1958, 2874, 2985, 3579
Bme18I GGWCC 5 cut(s) 34, 1661, 1922, 2375, 2870
BmeRI GACNNNNNGTC 1 cut(s) 1982
BmgBI CACGTC 2 cut(s) 1326, 3547
BmgT120I GGNCC 6 cut(s) 34, 1510, 1661, 1922, 2375, 2870
BmiI GGNNCC 6 cut(s) 1265, 1511, 1672, 2199, 3117, 4042
BmrFI CCNGG 6 cut(s) 1020, 1368, 1958, 2874, 2985, 3579
BmrI ACTGGG 1 cut(s) 2892
BmtI GCTAGC 1 cut(s) 2915
BmuI ACTGGG 1 cut(s) 2892
BoxI GACNNNNGTC 1 cut(s) 976
BpiI GAAGAC 7 cut(s) 865, 976, 1446, 1753, 2136, 2894, 3417
BplI GAGNNNNNCTC 2 cut(s) 2983, 3015
BpmI CTGGAG 2 cut(s) 1806, 2895
Bpu14I TTCGAA 3 cut(s) 293, 685, 2533
BpuEI CTTGAG 2 cut(s) 1445, 3939
BpuMI CCSGG 3 cut(s) 1958, 2985, 3579
Bsa29I ATCGAT 2 cut(s) 595, 2865
BsaAI YACGTR 4 cut(s) 1084, 1247, 1778, 2351
BsaBI GATNNNNATC 2 cut(s) 1029, 1731
BsaI GGTCTC 2 cut(s) 266, 535
BsaJI CCNNGG 5 cut(s) 691, 1018, 1366, 1829, 4045
BsaWI WCCGGW 1 cut(s) 2200
Bse1I ACTGG 5 cut(s) 633, 1766, 2530, 2887, 3917
Bse3DI GCAATG 2 cut(s) 256, 2906
Bse8I GATNNNNATC 2 cut(s) 1029, 1731
BseAI TCCGGA 1 cut(s) 2200
BseBI CCWGG 3 cut(s) 1020, 1368, 2874
BseCI ATCGAT 2 cut(s) 595, 2865
BseDI CCNNGG 5 cut(s) 691, 1018, 1366, 1829, 4045
BseGI GGATG 5 cut(s) 203, 1549, 3038, 3115, 3181
BseJI GATNNNNATC 2 cut(s) 1029, 1731
BseMI GCAATG 2 cut(s) 256, 2906
BseMII CTCAG 4 cut(s) 81, 1120, 3798, 3917
BseNI ACTGG 5 cut(s) 633, 1766, 2530, 2887, 3917
BseRI GAGGAG 1 cut(s) 4053
BseSI GKGCMC 4 cut(s) 490, 628, 2008, 3307
BseXI GCAGC 7 cut(s) 132, 135, 751, 775, 1441, 2956, 3663
BseYI CCCAGC 2 cut(s) 3830, 3881
BsgI GTGCAG 1 cut(s) 3891
Bsh1285I CGRYCG 1 cut(s) 885
BshFI GGCC 4 cut(s) 1512, 2456, 2828, 4059
BshNI GGYRCC 2 cut(s) 1263, 3115
BshVI ATCGAT 2 cut(s) 595, 2865
BsiEI CGRYCG 1 cut(s) 885
BsiHKAI GWGCWC 4 cut(s) 490, 2008, 2666, 3712
BsiSI CCGG 4 cut(s) 1958, 2201, 2985, 3578
BslFI GGGAC 4 cut(s) 191, 1754, 2558, 3847
BsmAI GTCTC 7 cut(s) 228, 266, 535, 1782, 2114, 3159, 3322
BsmFI GGGAC 4 cut(s) 191, 1754, 2558, 3847
BsmI GAATGC 2 cut(s) 2475, 2810
BsnI GGCC 4 cut(s) 1512, 2456, 2828, 4059
Bso31I GGTCTC 2 cut(s) 266, 535
Bsp119I TTCGAA 3 cut(s) 293, 685, 2533
Bsp1286I GDGCHC 7 cut(s) 490, 628, 2008, 2666, 3307, 3712, 4045
Bsp13I TCCGGA 1 cut(s) 2200
Bsp1407I TGTACA 1 cut(s) 1522
BspACI CCGC 9 cut(s) 142, 329, 1047, 1582, 2438, 2829, 2988, 3666, 4056
BspANI GGCC 4 cut(s) 1512, 2456, 2828, 4059
BspCNI CTCAG 4 cut(s) 82, 1119, 3799, 3918
BspDI ATCGAT 2 cut(s) 595, 2865
BspEI TCCGGA 1 cut(s) 2200
BspHI TCATGA 3 cut(s) 67, 955, 2562
BspLI GGNNCC 6 cut(s) 1265, 1511, 1672, 2199, 3117, 4042
BspMI ACCTGC 2 cut(s) 1184, 3659
BspOI GCTAGC 1 cut(s) 2915
BspPI GGATC 6 cut(s) 81, 120, 334, 2192, 2205, 3336
BspQI GCTCTTC 1 cut(s) 1611
BspT104I TTCGAA 3 cut(s) 293, 685, 2533
BspT107I GGYRCC 2 cut(s) 1263, 3115
BspTNI GGTCTC 2 cut(s) 266, 535
BsrDI GCAATG 2 cut(s) 256, 2906
BsrGI TGTACA 1 cut(s) 1522
BsrI ACTGG 5 cut(s) 633, 1766, 2530, 2887, 3917
BssECI CCNNGG 5 cut(s) 691, 1018, 1366, 1829, 4045
Bst2UI CCWGG 3 cut(s) 1020, 1368, 2874
Bst6I CTCTTC 4 cut(s) 823, 1462, 1611, 2836
BstAPI GCANNNNNTGC 1 cut(s) 3878
BstAUI TGTACA 1 cut(s) 1522
BstBAI YACGTR 4 cut(s) 1084, 1247, 1778, 2351
BstBI TTCGAA 3 cut(s) 293, 685, 2533
BstC8I GCNNGC 4 cut(s) 722, 2151, 2913, 4054
BstDEI CTNAG 6 cut(s) 90, 230, 1106, 2944, 3807, 3926
BstDSI CCRYGG 2 cut(s) 1829, 4045
BstF5I GGATG 5 cut(s) 203, 1549, 3038, 3115, 3181
BstH2I RGCGCY 1 cut(s) 703
BstHHI GCGC 4 cut(s) 120, 179, 702, 1017
BstMAI GTCTC 7 cut(s) 228, 266, 535, 1782, 2114, 3159, 3322
BstMCI CGRYCG 1 cut(s) 885
BstMWI GCNNNNNNNGC 5 cut(s) 2012, 2912, 3878, 4049, 4065
BstNI CCWGG 3 cut(s) 1020, 1368, 2874
BstNSI RCATGY 8 cut(s) 240, 440, 1523, 1531, 2095, 2153, 3137, 3977
BstPAI GACNNNNGTC 1 cut(s) 976
BstSCI CCNGG 6 cut(s) 1018, 1366, 1956, 2872, 2983, 3577
BstSFI CTRYAG 2 cut(s) 2124, 2242
BstSLI GKGCMC 4 cut(s) 490, 628, 2008, 3307
BstSNI TACGTA 1 cut(s) 1778
BstV1I GCAGC 7 cut(s) 132, 135, 751, 775, 1441, 2956, 3663
BstV2I GAAGAC 7 cut(s) 865, 976, 1446, 1753, 2136, 2894, 3417
BstX2I RGATCY 4 cut(s) 1781, 2197, 2629, 3341
BstYI RGATCY 4 cut(s) 1781, 2197, 2629, 3341
Bsu15I ATCGAT 2 cut(s) 595, 2865
BsuI GTATCC 1 cut(s) 918
BsuRI GGCC 4 cut(s) 1512, 2456, 2828, 4059
BsuTUI ATCGAT 2 cut(s) 595, 2865
BtgI CCRYGG 2 cut(s) 1829, 4045
BtgZI GCGATG 2 cut(s) 1289, 3402
BtrI CACGTC 2 cut(s) 1326, 3547
BtsCI GGATG 5 cut(s) 203, 1549, 3038, 3115, 3181
BtsI GCAGTG 3 cut(s) 133, 144, 3093
BtsIMutI CAGTG 7 cut(s) 87, 133, 144, 626, 1710, 2320, 3093
BveI ACCTGC 2 cut(s) 1184, 3659
Cac8I GCNNGC 4 cut(s) 722, 2151, 2913, 4054
CciI TCATGA 3 cut(s) 67, 955, 2562
CfoI GCGC 4 cut(s) 120, 179, 702, 1017
Cfr13I GGNCC 6 cut(s) 34, 1510, 1661, 1922, 2375, 2870
ClaI ATCGAT 2 cut(s) 595, 2865
CseI GACGC 1 cut(s) 143
DdeI CTNAG 6 cut(s) 90, 230, 1106, 2944, 3807, 3926
DraI TTTAAA 1 cut(s) 2032
DrdI GACNNNNNNGTC 1 cut(s) 1755
DriI GACNNNNNGTC 1 cut(s) 1982
DseDI GACNNNNNNGTC 1 cut(s) 1755
EaeI YGGCCR 1 cut(s) 2826
Eam1104I CTCTTC 4 cut(s) 823, 1462, 1611, 2836
Eam1105I GACNNNNNGTC 1 cut(s) 1982
EarI CTCTTC 4 cut(s) 823, 1462, 1611, 2836
EciI GGCGGA 2 cut(s) 318, 1062
Eco105I TACGTA 1 cut(s) 1778
Eco147I AGGCCT 1 cut(s) 2456
Eco24I GRGCYC 1 cut(s) 4045
Eco31I GGTCTC 2 cut(s) 266, 535
Eco32I GATATC 2 cut(s) 551, 2216
Eco47I GGWCC 5 cut(s) 34, 1661, 1922, 2375, 2870
Eco57I CTGAAG 2 cut(s) 1590, 2349
Eco72I CACGTG 1 cut(s) 1247
EcoO109I RGGNCCY 2 cut(s) 1510, 1922
EcoRI GAATTC 2 cut(s) 2228, 3855
EcoRII CCWGG 3 cut(s) 1018, 1366, 2872
EcoRV GATATC 2 cut(s) 551, 2216
EcoT22I ATGCAT 3 cut(s) 1000, 1732, 3037
EcoT38I GRGCYC 1 cut(s) 4045
FalI AAGNNNNNCTT 2 cut(s) 2879, 2911
FaqI GGGAC 4 cut(s) 191, 1754, 2558, 3847
FauI CCCGC 1 cut(s) 2981
FauNDI CATATG 3 cut(s) 54, 934, 1650
FbaI TGATCA 1 cut(s) 3478
FblI GTMKAC 2 cut(s) 1914, 2064
FokI GGATG 5 cut(s) 210, 1556, 3025, 3122, 3188
FriOI GRGCYC 1 cut(s) 4045
GlaI GCGC 4 cut(s) 119, 178, 701, 1016
GsaI CCCAGC 2 cut(s) 3834, 3885
GsuI CTGGAG 2 cut(s) 1806, 2895
HaeII RGCGCY 1 cut(s) 703
HaeIII GGCC 4 cut(s) 1512, 2456, 2828, 4059
HapII CCGG 4 cut(s) 1958, 2201, 2985, 3578
HgaI GACGC 1 cut(s) 143
HhaI GCGC 4 cut(s) 120, 179, 702, 1017
Hin6I GCGC 4 cut(s) 118, 177, 700, 1015
HinP1I GCGC 4 cut(s) 118, 177, 700, 1015
HincII GTYRAC 4 cut(s) 133, 2065, 2519, 3693
HindII GTYRAC 4 cut(s) 133, 2065, 2519, 3693
HindIII AAGCTT 3 cut(s) 558, 1556, 2265
HpaI GTTAAC 1 cut(s) 3693
HpaII CCGG 4 cut(s) 1958, 2201, 2985, 3578
HphI GGTGA 9 cut(s) 70, 1034, 1441, 2173, 2284, 2313, 2345, 3488, 3593
HpyAV CCTTC 6 cut(s) 286, 527, 753, 2274, 2324, 2366
HpyCH4IV ACGT 8 cut(s) 754, 1083, 1246, 1325, 1777, 2350, 2891, 3546
HpyF10VI GCNNNNNNNGC 5 cut(s) 2012, 2912, 3878, 4049, 4065
HpyF3I CTNAG 6 cut(s) 90, 230, 1106, 2944, 3807, 3926
HpySE526I ACGT 8 cut(s) 754, 1083, 1246, 1325, 1777, 2350, 2891, 3546
HspAI GCGC 4 cut(s) 118, 177, 700, 1015
Kpn2I TCCGGA 1 cut(s) 2200
KpnI GGTACC 1 cut(s) 1267
Ksp22I TGATCA 1 cut(s) 3478
KspAI GTTAAC 1 cut(s) 3693
LguI GCTCTTC 1 cut(s) 1611
LmnI GCTCC 3 cut(s) 1878, 3715, 4040
Lsp1109I GCAGC 7 cut(s) 132, 135, 751, 775, 1441, 2956, 3663
MaeII ACGT 8 cut(s) 754, 1083, 1246, 1325, 1777, 2350, 2891, 3546
MaeIII GTNAC 8 cut(s) 10, 1005, 1121, 1196, 1328, 1853, 3428, 3755
MfeI CAATTG 2 cut(s) 715, 2778
MflI RGATCY 4 cut(s) 1781, 2197, 2629, 3341
MhlI GDGCHC 7 cut(s) 490, 628, 2008, 2666, 3307, 3712, 4045
MlyI GAGTC 4 cut(s) 855, 872, 1160, 3602
MmeI TCCRAC 4 cut(s) 865, 1216, 1521, 2081
Mph1103I ATGCAT 3 cut(s) 1000, 1732, 3037
MroI TCCGGA 1 cut(s) 2200
MroXI GAANNNNTTC 2 cut(s) 2133, 3270
MspI CCGG 4 cut(s) 1958, 2201, 2985, 3578
MspR9I CCNGG 6 cut(s) 1020, 1368, 1958, 2874, 2985, 3579
MunI CAATTG 2 cut(s) 715, 2778
Mva1269I GAATGC 2 cut(s) 2475, 2810
MvaI CCWGG 3 cut(s) 1020, 1368, 2874
MwoI GCNNNNNNNGC 5 cut(s) 2012, 2912, 3878, 4049, 4065
NciI CCSGG 3 cut(s) 1958, 2985, 3579
NdeI CATATG 3 cut(s) 54, 934, 1650
NheI GCTAGC 1 cut(s) 2911
NlaIV GGNNCC 6 cut(s) 1265, 1511, 1672, 2199, 3117, 4042
NmuCI GTSAC 3 cut(s) 10, 1853, 3428
NsiI ATGCAT 3 cut(s) 1000, 1732, 3037
NspI RCATGY 8 cut(s) 240, 440, 1523, 1531, 2095, 2153, 3137, 3977
NspV TTCGAA 3 cut(s) 293, 685, 2533
OliI CACNNNNGTG 1 cut(s) 1796
PaeI GCATGC 1 cut(s) 2153
PagI TCATGA 3 cut(s) 67, 955, 2562
PaqCI CACCTGC 1 cut(s) 3659
PceI AGGCCT 1 cut(s) 2456
PciI ACATGT 4 cut(s) 236, 1519, 3133, 3973
PciSI GCTCTTC 1 cut(s) 1611
PcsI WCGNNNNNNNCGW 3 cut(s) 891, 1331, 3160
PctI GAATGC 2 cut(s) 2475, 2810
PdmI GAANNNNTTC 2 cut(s) 2133, 3270
PfeI GAWTC 9 cut(s) 446, 655, 1094, 1654, 2106, 2932, 3009, 3016, 3089
PflFI GACNNNGTC 1 cut(s) 881
PfoI TCCNGGA 1 cut(s) 2872
PleI GAGTC 4 cut(s) 855, 872, 1160, 3602
PmaCI CACGTG 1 cut(s) 1247
PmlI CACGTG 1 cut(s) 1247
PpsI GAGTC 4 cut(s) 855, 872, 1160, 3602
Ppu21I YACGTR 4 cut(s) 1084, 1247, 1778, 2351
PpuMI RGGWCCY 1 cut(s) 1922
PscI ACATGT 4 cut(s) 236, 1519, 3133, 3973
PshAI GACNNNNGTC 1 cut(s) 976
PshBI ATTAAT 3 cut(s) 453, 948, 2406
PsiI TTATAA 1 cut(s) 1824
Psp5II RGGWCCY 1 cut(s) 1922
Psp6I CCWGG 3 cut(s) 1018, 1366, 2872
PspCI CACGTG 1 cut(s) 1247
PspFI CCCAGC 2 cut(s) 3830, 3881
PspGI CCWGG 3 cut(s) 1018, 1366, 2872
PspN4I GGNNCC 6 cut(s) 1265, 1511, 1672, 2199, 3117, 4042
PspPI GGNCC 6 cut(s) 34, 1510, 1661, 1922, 2375, 2870
PspPPI RGGWCCY 1 cut(s) 1922
PsuI RGATCY 4 cut(s) 1781, 2197, 2629, 3341
PsyI GACNNNGTC 1 cut(s) 881
SalI GTCGAC 1 cut(s) 2063
SapI GCTCTTC 1 cut(s) 1611
Sau96I GGNCC 6 cut(s) 34, 1510, 1661, 1922, 2375, 2870
SchI GAGTC 4 cut(s) 855, 872, 1160, 3602
ScrFI CCNGG 6 cut(s) 1020, 1368, 1958, 2874, 2985, 3579
SduI GDGCHC 7 cut(s) 490, 628, 2008, 2666, 3307, 3712, 4045
SfcI CTRYAG 2 cut(s) 2124, 2242
SfuI TTCGAA 3 cut(s) 293, 685, 2533
SinI GGWCC 5 cut(s) 34, 1661, 1922, 2375, 2870
SmiI ATTTAAAT 1 cut(s) 2032
SmlI CTYRAG 2 cut(s) 1424, 3954
SmoI CTYRAG 2 cut(s) 1424, 3954
SnaBI TACGTA 1 cut(s) 1778
SphI GCATGC 1 cut(s) 2153
SseBI AGGCCT 1 cut(s) 2456
SsiI CCGC 9 cut(s) 142, 329, 1047, 1582, 2438, 2829, 2988, 3666, 4056
SspI AATATT 4 cut(s) 304, 1685, 2177, 3733
StuI AGGCCT 1 cut(s) 2456
StyD4I CCNGG 6 cut(s) 1018, 1366, 1956, 2872, 2983, 3577
SwaI ATTTAAAT 1 cut(s) 2032
TaiI ACGT 8 cut(s) 757, 1086, 1249, 1328, 1780, 2353, 2894, 3549
TaqII GACCGA 3 cut(s) 871, 1193, 2392
TatI WGTACW 2 cut(s) 1522, 3597
TauI GCSGC 4 cut(s) 145, 2441, 2831, 4059
TfiI GAWTC 9 cut(s) 446, 655, 1094, 1654, 2106, 2932, 3009, 3016, 3089
TscAI CASTG 7 cut(s) 94, 133, 144, 633, 1717, 2320, 3100
TseFI GTSAC 3 cut(s) 10, 1853, 3428
TseI GCWGC 7 cut(s) 120, 123, 739, 788, 1429, 2969, 3676
Tsp45I GTSAC 3 cut(s) 10, 1853, 3428
TspRI CASTG 7 cut(s) 94, 133, 144, 633, 1717, 2320, 3100
Tth111I GACNNNGTC 1 cut(s) 881
VneI GTGCAC 2 cut(s) 486, 2004
VpaK11BI GGWCC 5 cut(s) 34, 1661, 1922, 2375, 2870
VspI ATTAAT 3 cut(s) 453, 948, 2406
XbaI TCTAGA 1 cut(s) 3005
XceI RCATGY 8 cut(s) 240, 440, 1523, 1531, 2095, 2153, 3137, 3977
XcmI CCANNNNNNNNNTGG 4 cut(s) 851, 1459, 2016, 2191
XmiI GTMKAC 2 cut(s) 1914, 2064
XmnI GAANNNNTTC 2 cut(s) 2133, 3270
Zsp2I ATGCAT 3 cut(s) 1000, 1732, 3037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.