Rw0G001510

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00044
OG0000080
Physical Location & Seq
Forward (+)
10809 .. 15982
5174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001510.1

Sequence Viewer

Length: 4368 bp
ATGGAGCATGTGCATGCTCGGCACCGACAATGGCGAGAGTCAATGAGTTCTTCACAACGTCAAGCATACTTGGCACGACGACCCGCAATAGCTCAAGGAAAGCGACCCGTGGTGGAAACTAGCCCAAGTAACAGAGCTGAACAAGCTCCAGAAGAATTTGACAATGAAGGCACAAGTTCAAGAATTTCTTCAAATTCCAACAACCCCGAACAAGACTACACAAGGGTTCGCTTAACTAGCATAAGACAAATGGCACGTTTGAATAGATCAGAAACAAGCGATGGAGTTGTCCGAATTTCTACTTGCTATGAGTTACCTAATGGTACCATGAATAATCATGAGGAGCATAACAGTAACAATGTAGCCATGAATAATGATGAGGAACATACCAACAACATTGAGGAAGACAATGAAAGTGAGCCCATGAATAATCATGAGGAGCGCCGTAACAACATTCATCAAGGGAATATTGTTCTACCACTAATACAGTCTCCCCCAGAAATGGTTCACCTCTTTTCTGATCAAAATGATAAGGGCAGAAAATTCAGACAAAATATTAGGGCCTACAACAATGTGTTTGCATTCACTTCAATGGGCGTACATGTTGATGAAAGAATTAATATCCAAGGCCATGGACCTTATACATTTGGTGCACAAGGTTCTATATATCATAAAATTGGTGGTCTATTACCAATTGACGGGAGAAGACCACGATATCTACAGGCTTATATATACGACACTGAGCATGAACTTGAGAATAGATTGTCTGAAAGCGAAGTTTTGGAGAGGGGAATGGTTGAAAAGATACAACAAATTCTTAATCAACACAATCCATTTGTTCAAACATTTCGAAGTCTCGGACAACGTCAAGATTTGCCCAATTGCAGACTCATCATCAGAGAGCAACCTTCCGACCGCCGTCAGTATAATTTGCCATCTGCATCACAAGTTGCTGCTATTATAGTAGGGGGTGACGACATCAATCCAAATGGGAGAGATTTTATTGTTCAAACAATTAGTGGACAACTTTGGAATGTCAAAGACTCAGTTGGATATTATGACCCCATGCAATATCCGTTATTATTGCCTTATGGAACATACGGATGGGCCATTAATACTCATGTCAATAATGGAAGGCCAATGTCATGTTGTGACTACTACGCATATATGTTACAGATGCGTCAAGGTCATGAAAATCCTCTACATCGAGGAGGACGCCTCTTCCAACAGTATGTTGTAGATAATTATGTAAAGATTGAATCACAGAAGCTCAGATGGCTTCGTAGTAATCAAGAAAAATTTCGGAGAGAATTTCGTCAAGGACTTCAAGACTCTCTACATGCAGGAGAAAATGATGCAGGTAACGTTGGTCGTAGAACAATTCTGCCATCATCGTTTATTGGAAGCCCACGTGACATGTACCAACGATATCAAGATGCAATGGCTTTGGTTCAAAAATATGGTAGGCCGGATCTTTTTATCACCATGACCTGTAACCCCAATTGGGAAGAAGTTAGAAATGAACTACTCCCTGGACAAACGCCACAAGACCGTCCTGATTTAGTCACCAGGGTATTTCATGCCAAGTTTGAGCAATTAAAAGAAGACATCATCAACAAGGGGGTGCTGGGAAAAGTGGCTGCACATGCTTTCGTTGTTGAATTTCAAAAACGGGGCCTACCACATGTTCATATGCTGATTATGTTAGAAGAAAATGACAAGCTTAATAACCCTGATGAATATGATCGCATTGTTAGAGCTGAAATACCATATGAGGACGAGGAGCCTCAACTTTATGATGCAGTGTGTACACATATGATTCATGGTCCATGTGGAACTCTTAACCCAAGGCAGTCATGTATGAAAAATGGAAGTTGCAATAAAGGCTACCCAAAACCATTTGCAAATTTCACTGTGCAAGGGAACGATGCATATCCTGTATATCGGAGACGAGCAAGTCGTTTACCAGTACCTTCGCGTAGACGAGGAGATGTGATGGTGGATAACGGTTGGGTGGTTCCCTACAACCCATGGTTGCTATTACGGTATAATTGTCACATCAATGTTGAAATATGTGGGAGCGTAAAAAGTGTCAAGTACTTATATAAGTATATCTACAAAGGTCCTGACAGAGTGGCACTTGAGCTGCAATCAAACCCAGAGTTTGATGAAATTAAACAATTTGTTAATGCAAGGTGGGTTTGTGCACCGGAGGCTTTATGGAGGATCTTCAAATTTGTAATGAATAGAATATATCCAACAGTAGAGCGATTACAAATACATCTTCCCAACATGCAACAGATAACATTCAGTGTTGATGAAACAGTAGAGCATATTCTGGCAGATGAGCACGCACAAATGTCAATGCTTACTGAGTTTTTCACCATAAATCGAATGGATGAGGATGCAAGAGCATATTTGTATCAAGAAATTCCTGAACATTACAGATGGGATAATAGCAACAAAATTTGGGTGAAAAGAAGAAGAAACTACAAGGTTATTGGGCGAATATACAGCGTCTCACCGTCTGAAGGTGAGAAATTTTACATGCGTGTCCTTCTTAATCATGTAAGAGGTCCAAGATCATTTCATGATTTGCTTATTGTTAATGGTGTTCTACAACCAACTTTCAAGCAAGCAGCAGAGAAACAAGGTTTGCTAGAAAATGATAATAGTATCCGACAGTGTTTATTGGAGGCCTCTGATATTAGGATGCCATCAACTTTGAGGAGATTATTAGTCACAATTTTAGTGTACAATGAGCCAATAGGTGTCCGAACATTATGGGATGAATTTTTTCCTTTTATGGTGGAAGATTATCCTTCATCTGGTGATATTGCAAACCGAAACAGGCTCTTACAAGATTTAAATGGAGTACTTGAACAATTTAACAGGAACATTAGAGATTTTGACCTGCCTGAAATGACAACAGTGCCAGAAGAAAATTCAAGAATGCCAAGGTGTATTGAAGACGAACTTTCCATACATATCTCTCAAGAAGACATTGATGCAATTGACCGCCTTAATGACGACCAAAGGATCGCCTTTAACACGAACATGGATGCAGTTCAGAGAAAAAGAAGTGCATTATTTTTTGTTGATGGTCCTGGTGGAACTGGGAAGACATATTTATACGGTGCATTGCTAGCAAACATACAAAGAATGAATCATATAGTCTTAGCCACAACATCATCTGGAATAGCTGTAAATATACTGCCCGGCAGGAGGACAGCTCACTCCAGATTTAAGATTCCACTCAATGTAGATGCTTCATCTATGTGTTCCATAAGTAAACAGTCTGCTCTGGCACAACTGGTACAAGATTCGACTGCAATTATTTGGGATGAGGCACCTATGACACATCGACATGTCTTTGAAGCACTTGATCGAACTTTTAGAGACATAATGGATGTCGACTTACCATTTGGTGGAAAGATAATGATTTTTGGGGGAGATTTTCGTCAAGTCCTCCCTGTTATTCCTAAAGGAACGAAGTCTAAACTCATTCAAGCCTCCATTGTGAAATCAACATTTTGGACACAGATAAAGATATTGAGACTGAGACAGAATATGAGATCCATAAATGACCACCAATTTGCAGAGTTTCTTCTTCGTGTAGGCGATGGGAGTGAACAAGTCATCAATGACGAAATGATAAGATTACCGGAATGCATGGTGGTTCCATGGGAAAGCGACCAATCTATCAATCTAATTATTGATGAAGTTTTCCCCAATTTGGGTGATCATGTAAATGATGCAAGATACATGGTGGACAGAGCATTGATTACTCCTATAAACGACGATGTGGACGTGTTGAATGAAAAGATAATCCATATGTTTCCAGGTGAAGAAATTACATTGTACTCATTTGACTCTGTTGAAGATGACATCAGAAATTTGTACCAACCAGAGTTTCTGAACTCAATAACCGCAGGTGGGCTGCCACCCCACAAGTTAACTCTGAAAGTAGGTGCTCCAATCATGCTTTTGAGGAATATTGACCCTAAATTGGGATTGTGTAACGGTACTAGATTGTTGTGCCGTGGTTCGTACCAAAATCTGATTGATGCTGAGCTACTAACAGGACAATTTGCTGGGACTAGAGTTTTTCTACCTAGAATTCCTTTGAAAAGTGCAGAAAATGCTGGGCTCCCATTTCAACTAACAAGAAAGCAATTTCCAGTGAAACTGAGCTTATGTTTGACCATAAACAAATCTCAAGGCCAGACTATCCCACATGTAGGTGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTATATGTGGCATTGTCGAGAGGAGTGAATGGTACCAGATCGTCGCCCTCAACAAAAGAAAAAGTTCTAAAGAGACAAGGGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1455

Amino Acids

167.28

Weight (kDa)

6.71

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 386 - 567 8e-66 Helitron helicase-like domain at N-terminus
PIF1 PF05970 1008 - 1213 1.9e-71 PIF1-like helicase
Pif1_2B_dom PF21530 1304 - 1346 1.1e-20 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3923
AasI GACNNNNNNGTC 1 cut(s) 1956
Acc36I ACCTGC 3 cut(s) 1349, 2922, 3923
Acc65I GGTACC 2 cut(s) 323, 4313
AccB1I GGYRCC 4 cut(s) 21, 323, 3349, 4313
AccB7I CCANNNNNTGG 1 cut(s) 3428
AccI GTMKAC 2 cut(s) 1981, 3414
AccII CGCG 1 cut(s) 1978
AciI CCGC 4 cut(s) 84, 916, 3019, 3930
AclI AACGTT 1 cut(s) 1365
AclWI GGATC 4 cut(s) 1479, 2234, 3047, 3570
AcuI CTGAAG 1 cut(s) 2550
AcvI CACGTG 1 cut(s) 1412
AcyI GRCGYC 1 cut(s) 1216
AflIII ACRYGT 6 cut(s) 601, 1416, 1684, 3367, 3810, 4237
AjiI CACGTC 1 cut(s) 3811
AjnI CCWGG 4 cut(s) 1531, 1568, 3106, 3841
AjuI GAANNNNNNNTTGG 2 cut(s) 3897, 3929
Alw21I GWGCWC 4 cut(s) 655, 2209, 2352, 3976
Alw26I GTCTC 8 cut(s) 495, 860, 1942, 2524, 3393, 3550, 3556, 4348
Alw44I GTGCAC 2 cut(s) 651, 2205
AlwI GGATC 4 cut(s) 1479, 2234, 3047, 3570
AoxI GGCC 9 cut(s) 561, 628, 1107, 1136, 1466, 1675, 2697, 4222, 4361
ApaLI GTGCAC 2 cut(s) 651, 2205
ApeKI GCWGC 5 cut(s) 953, 1640, 2146, 2639, 3940
ArsI GACNNNNNNTTYG 4 cut(s) 3407, 3439, 3578, 3610
AseI ATTAAT 2 cut(s) 618, 1113
Asp700I GAANNNNTTC 5 cut(s) 187, 504, 1299, 2796, 4344
Asp718I GGTACC 2 cut(s) 323, 4313
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 9 cut(s) 561, 635, 1107, 1675, 1826, 2123, 2576, 3104, 4361
AsuC2I CCSGG 1 cut(s) 3219
AsuII TTCGAA 1 cut(s) 850
AsuNHI GCTAGC 1 cut(s) 3145
AvaII GGWCC 5 cut(s) 635, 1826, 2123, 2576, 3104
BaeGI GKGCMC 2 cut(s) 655, 2209
BanI GGYRCC 4 cut(s) 21, 323, 3349, 4313
BanII GRGCYC 2 cut(s) 423, 4152
BbrPI CACGTG 1 cut(s) 1412
BbsI GAAGAC 6 cut(s) 411, 712, 1611, 2976, 3006, 3128
Bbv12I GWGCWC 4 cut(s) 655, 2209, 2352, 3976
BbvI GCAGC 5 cut(s) 940, 1627, 2133, 2651, 3927
BceAI ACGGC 3 cut(s) 429, 903, 4026
BciT130I CCWGG 4 cut(s) 1533, 1570, 3108, 3843
BciVI GTATCC 1 cut(s) 2687
BclI TGATCA 2 cut(s) 520, 3742
BcnI CCSGG 1 cut(s) 3219
BcoDI GTCTC 8 cut(s) 495, 860, 1942, 2524, 3393, 3550, 3556, 4348
BfaI CTAG 7 cut(s) 120, 237, 2660, 3146, 4029, 4101, 4116
BfmI CTRYAG 1 cut(s) 719
BfoI RGCGCY 1 cut(s) 445
BfuAI ACCTGC 3 cut(s) 1349, 2922, 3923
BfuI GTATCC 1 cut(s) 2687
BisI GCNGC 5 cut(s) 954, 1641, 2147, 2640, 3941
BlpI GCTNAGC 1 cut(s) 4071
BlsI GCNGC 5 cut(s) 955, 1642, 2148, 2641, 3942
BmcAI AGTACT 2 cut(s) 2099, 2877
Bme1390I CCNGG 5 cut(s) 1533, 1570, 3108, 3219, 3843
Bme18I GGWCC 5 cut(s) 635, 1826, 2123, 2576, 3104
BmgBI CACGTC 1 cut(s) 3811
BmgT120I GGNCC 9 cut(s) 561, 635, 1107, 1675, 1826, 2123, 2576, 3104, 4361
BmiI GGNNCC 9 cut(s) 23, 325, 1676, 1785, 2019, 3351, 3681, 4151, 4315
BmrFI CCNGG 5 cut(s) 1533, 1570, 3108, 3219, 3843
BmrI ACTGGG 1 cut(s) 3126
BmtI GCTAGC 1 cut(s) 3149
BmuI ACTGGG 1 cut(s) 3126
BoxI GACNNNNGTC 1 cut(s) 918
BpiI GAAGAC 6 cut(s) 411, 712, 1611, 2976, 3006, 3128
BplI GAGNNNNNCTC 4 cut(s) 1203, 1235, 3217, 3249
BpmI CTGGAG 2 cut(s) 132, 3223
Bpu1102I GCTNAGC 1 cut(s) 4071
Bpu14I TTCGAA 1 cut(s) 850
BpuEI CTTGAG 5 cut(s) 78, 773, 2162, 2979, 4203
BpuMI CCSGG 1 cut(s) 3219
BsaAI YACGTR 1 cut(s) 1412
BsaBI GATNNNNATC 1 cut(s) 1932
BsaHI GRCGYC 1 cut(s) 1216
BsaWI WCCGGW 2 cut(s) 2209, 3664
Bse1I ACTGG 4 cut(s) 1967, 3121, 3318, 4181
Bse3DI GCAATG 2 cut(s) 1446, 3140
Bse8I GATNNNNATC 1 cut(s) 1932
BseBI CCWGG 4 cut(s) 1533, 1570, 3108, 3843
BseGI GGATG 8 cut(s) 1109, 2404, 2410, 2718, 2794, 3067, 3349, 3415
BseJI GATNNNNATC 1 cut(s) 1932
BseMI GCAATG 2 cut(s) 1446, 3140
BseMII CTCAG 7 cut(s) 732, 1059, 1285, 2364, 3551, 4062, 4181
BseNI ACTGG 4 cut(s) 1967, 3121, 3318, 4181
BseRI GAGGAG 7 cut(s) 356, 452, 1224, 1796, 2001, 2743, 4317
BseSI GKGCMC 2 cut(s) 655, 2209
BseXI GCAGC 5 cut(s) 940, 1627, 2133, 2651, 3927
BseYI CCCAGC 3 cut(s) 1627, 4094, 4145
BsgI GTGCAG 2 cut(s) 1626, 4155
Bsh1236I CGCG 1 cut(s) 1978
Bsh1285I CGRYCG 1 cut(s) 916
BshFI GGCC 9 cut(s) 563, 630, 1109, 1138, 1468, 1677, 2699, 4224, 4363
BshNI GGYRCC 4 cut(s) 21, 323, 3349, 4313
BsiEI CGRYCG 1 cut(s) 916
BsiHKAI GWGCWC 4 cut(s) 655, 2209, 2352, 3976
BsiSI CCGG 4 cut(s) 1469, 2210, 3219, 3665
BslFI GGGAC 1 cut(s) 4111
BsmAI GTCTC 8 cut(s) 495, 860, 1942, 2524, 3393, 3550, 3556, 4348
BsmBI CGTCTC 2 cut(s) 1942, 2524
BsmFI GGGAC 1 cut(s) 4111
BsmI GAATGC 3 cut(s) 581, 2958, 3674
BsnI GGCC 9 cut(s) 563, 630, 1109, 1138, 1468, 1677, 2699, 4224, 4363
Bsp119I TTCGAA 1 cut(s) 850
Bsp1286I GDGCHC 6 cut(s) 423, 655, 2209, 2352, 3976, 4152
Bsp1407I TGTACA 2 cut(s) 1808, 2754
Bsp1720I GCTNAGC 1 cut(s) 4071
Bsp19I CCATGG 3 cut(s) 631, 2030, 3683
BspACI CCGC 4 cut(s) 84, 916, 3019, 3930
BspANI GGCC 9 cut(s) 563, 630, 1109, 1138, 1468, 1677, 2699, 4224, 4363
BspCNI CTCAG 7 cut(s) 733, 1058, 1284, 2365, 3552, 4063, 4182
BspFNI CGCG 1 cut(s) 1978
BspHI TCATGA 4 cut(s) 337, 433, 1189, 2590
BspLI GGNNCC 9 cut(s) 23, 325, 1676, 1785, 2019, 3351, 3681, 4151, 4315
BspMI ACCTGC 3 cut(s) 1349, 2922, 3923
BspOI GCTAGC 1 cut(s) 3149
BspPI GGATC 4 cut(s) 1479, 2234, 3047, 3570
BspT104I TTCGAA 1 cut(s) 850
BspT107I GGYRCC 4 cut(s) 21, 323, 3349, 4313
BsrDI GCAATG 2 cut(s) 1446, 3140
BsrGI TGTACA 2 cut(s) 1808, 2754
BsrI ACTGG 4 cut(s) 1967, 3121, 3318, 4181
BssNI GRCGYC 1 cut(s) 1216
BssT1I CCWWGG 6 cut(s) 625, 631, 1847, 2030, 2957, 3683
Bst2UI CCWGG 4 cut(s) 1533, 1570, 3108, 3843
Bst6I CTCTTC 1 cut(s) 1226
BstACI GRCGYC 1 cut(s) 1216
BstAPI GCANNNNNTGC 1 cut(s) 4142
BstAUI TGTACA 2 cut(s) 1808, 2754
BstBAI YACGTR 1 cut(s) 1412
BstBI TTCGAA 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 15, 2352, 2637, 3147
BstDEI CTNAG 8 cut(s) 741, 1045, 1271, 2373, 3178, 3560, 4071, 4190
BstDSI CCRYGG 5 cut(s) 108, 631, 2030, 3683, 4042
BstF5I GGATG 8 cut(s) 1109, 2404, 2410, 2718, 2794, 3067, 3349, 3415
BstFNI CGCG 1 cut(s) 1978
BstH2I RGCGCY 1 cut(s) 445
BstHHI GCGC 1 cut(s) 444
BstMAI GTCTC 8 cut(s) 495, 860, 1942, 2524, 3393, 3550, 3556, 4348
BstMCI CGRYCG 1 cut(s) 916
BstNI CCWGG 4 cut(s) 1533, 1570, 3108, 3843
BstPAI GACNNNNGTC 1 cut(s) 918
BstSCI CCNGG 5 cut(s) 1531, 1568, 3106, 3217, 3841
BstSFI CTRYAG 1 cut(s) 719
BstSLI GKGCMC 2 cut(s) 655, 2209
BstUI CGCG 1 cut(s) 1978
BstV1I GCAGC 5 cut(s) 940, 1627, 2133, 2651, 3927
BstV2I GAAGAC 6 cut(s) 411, 712, 1611, 2976, 3006, 3128
BstX2I RGATCY 3 cut(s) 1471, 2226, 3575
BstXI CCANNNNNNTGG 1 cut(s) 632
BstYI RGATCY 3 cut(s) 1471, 2226, 3575
BsuI GTATCC 1 cut(s) 2687
BsuRI GGCC 9 cut(s) 563, 630, 1109, 1138, 1468, 1677, 2699, 4224, 4363
BtgI CCRYGG 5 cut(s) 108, 631, 2030, 3683, 4042
BtgZI GCGATG 2 cut(s) 294, 3636
BtrI CACGTC 1 cut(s) 3811
BtsCI GGATG 8 cut(s) 1109, 2404, 2410, 2718, 2794, 3067, 3349, 3415
BtsI GCAGTG 1 cut(s) 1809
BtsIMutI CAGTG 7 cut(s) 738, 1809, 1911, 2317, 2690, 2937, 4188
BveI ACCTGC 3 cut(s) 1349, 2922, 3923
Cac8I GCNNGC 4 cut(s) 15, 2352, 2637, 3147
CciI TCATGA 4 cut(s) 337, 433, 1189, 2590
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 9 cut(s) 561, 635, 1107, 1675, 1826, 2123, 2576, 3104, 4361
CseI GACGC 3 cut(s) 1169, 1224, 2506
DdeI CTNAG 8 cut(s) 741, 1045, 1271, 2373, 3178, 3560, 4071, 4190
DraI TTTAAA 1 cut(s) 2868
DrdI GACNNNNNNGTC 1 cut(s) 1956
DseDI GACNNNNNNGTC 1 cut(s) 1956
Eam1104I CTCTTC 1 cut(s) 1226
EarI CTCTTC 1 cut(s) 1226
Eco130I CCWWGG 6 cut(s) 625, 631, 1847, 2030, 2957, 3683
Eco147I AGGCCT 1 cut(s) 2699
Eco24I GRGCYC 2 cut(s) 423, 4152
Eco32I GATATC 2 cut(s) 716, 1430
Eco47I GGWCC 5 cut(s) 635, 1826, 2123, 2576, 3104
Eco57I CTGAAG 1 cut(s) 2550
Eco72I CACGTG 1 cut(s) 1412
EcoO109I RGGNCCY 3 cut(s) 561, 1675, 2123
EcoRI GAATTC 1 cut(s) 4119
EcoRII CCWGG 4 cut(s) 1531, 1568, 3106, 3841
EcoRV GATATC 2 cut(s) 716, 1430
EcoT14I CCWWGG 6 cut(s) 625, 631, 1847, 2030, 2957, 3683
EcoT22I ATGCAT 2 cut(s) 1933, 3674
EcoT38I GRGCYC 2 cut(s) 423, 4152
ErhI CCWWGG 6 cut(s) 625, 631, 1847, 2030, 2957, 3683
Esp3I CGTCTC 2 cut(s) 1942, 2524
FalI AAGNNNNNCTT 4 cut(s) 172, 204, 2961, 2993
FaqI GGGAC 1 cut(s) 4111
FauI CCCGC 1 cut(s) 91
FauNDI CATATG 4 cut(s) 1692, 1771, 1815, 3834
FbaI TGATCA 2 cut(s) 520, 3742
FblI GTMKAC 2 cut(s) 1981, 3414
Fnu4HI GCNGC 5 cut(s) 954, 1641, 2147, 2640, 3941
FokI GGATG 8 cut(s) 1116, 2411, 2417, 2725, 2801, 3074, 3356, 3422
FriOI GRGCYC 2 cut(s) 423, 4152
Fsp4HI GCNGC 5 cut(s) 954, 1641, 2147, 2640, 3941
FspBI CTAG 7 cut(s) 120, 237, 2660, 3146, 4029, 4101, 4116
GlaI GCGC 1 cut(s) 443
GluI GCNGC 5 cut(s) 954, 1641, 2147, 2640, 3941
GsaI CCCAGC 3 cut(s) 1631, 4098, 4149
GsuI CTGGAG 2 cut(s) 132, 3223
HaeII RGCGCY 1 cut(s) 445
HaeIII GGCC 9 cut(s) 563, 630, 1109, 1138, 1468, 1677, 2699, 4224, 4363
HapII CCGG 4 cut(s) 1469, 2210, 3219, 3665
HgaI GACGC 3 cut(s) 1169, 1224, 2506
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 1 cut(s) 1216
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 2 cut(s) 3415, 3957
HindII GTYRAC 2 cut(s) 3415, 3957
HindIII AAGCTT 1 cut(s) 1721
HpaI GTTAAC 1 cut(s) 3957
HpaII CCGG 4 cut(s) 1469, 2210, 3219, 3665
Hpy99I CGWCG 3 cut(s) 81, 3803, 4327
HpyAV CCTTC 7 cut(s) 161, 918, 1128, 1983, 2525, 2567, 2832
HpyCH4IV ACGT 6 cut(s) 58, 256, 865, 1365, 1411, 3810
HpyF3I CTNAG 8 cut(s) 741, 1045, 1271, 2373, 3178, 3560, 4071, 4190
HpySE526I ACGT 6 cut(s) 58, 256, 865, 1365, 1411, 3810
Hsp92I GRCGYC 1 cut(s) 1216
HspAI GCGC 1 cut(s) 442
KpnI GGTACC 2 cut(s) 327, 4317
Ksp22I TGATCA 2 cut(s) 520, 3742
KspAI GTTAAC 1 cut(s) 3957
LmnI GCTCC 8 cut(s) 4, 151, 343, 439, 1783, 2079, 3979, 4155
Lsp1109I GCAGC 5 cut(s) 940, 1627, 2133, 2651, 3927
MaeI CTAG 7 cut(s) 120, 237, 2660, 3146, 4029, 4101, 4116
MaeII ACGT 6 cut(s) 58, 256, 865, 1365, 1411, 3810
MfeI CAATTG 4 cut(s) 693, 880, 1501, 3012
MflI RGATCY 3 cut(s) 1471, 2226, 3575
MhlI GDGCHC 6 cut(s) 423, 655, 2209, 2352, 3976, 4152
MlyI GAGTC 5 cut(s) 47, 882, 1037, 1325, 3866
MmeI TCCRAC 6 cut(s) 222, 936, 1030, 1249, 2282, 2704
Mph1103I ATGCAT 2 cut(s) 1933, 3674
MroXI GAANNNNTTC 5 cut(s) 187, 504, 1299, 2796, 4344
MspI CCGG 4 cut(s) 1469, 2210, 3219, 3665
MspR9I CCNGG 5 cut(s) 1533, 1570, 3108, 3219, 3843
MunI CAATTG 4 cut(s) 693, 880, 1501, 3012
Mva1269I GAATGC 3 cut(s) 581, 2958, 3674
MvaI CCWGG 4 cut(s) 1533, 1570, 3108, 3843
MvnI CGCG 1 cut(s) 1978
NciI CCSGG 1 cut(s) 3219
NcoI CCATGG 3 cut(s) 631, 2030, 3683
NdeI CATATG 4 cut(s) 1692, 1771, 1815, 3834
NheI GCTAGC 1 cut(s) 3145
NlaIV GGNNCC 9 cut(s) 23, 325, 1676, 1785, 2019, 3351, 3681, 4151, 4315
NmuCI GTSAC 6 cut(s) 971, 1151, 1412, 1564, 2054, 2740
NsiI ATGCAT 2 cut(s) 1933, 3674
NspV TTCGAA 1 cut(s) 850
PaeI GCATGC 1 cut(s) 17
PagI TCATGA 4 cut(s) 337, 433, 1189, 2590
PaqCI CACCTGC 1 cut(s) 3923
PceI AGGCCT 1 cut(s) 2699
PciI ACATGT 5 cut(s) 601, 1416, 1684, 3367, 4237
PcsI WCGNNNNNNNCGW 2 cut(s) 1957, 3807
PctI GAATGC 3 cut(s) 581, 2958, 3674
PdmI GAANNNNTTC 5 cut(s) 187, 504, 1299, 2796, 4344
PfeI GAWTC 5 cut(s) 1259, 1819, 3166, 3250, 3323
PflFI GACNNNGTC 1 cut(s) 864
PflMI CCANNNNNTGG 1 cut(s) 3428
PkrI GCNGC 5 cut(s) 955, 1642, 2148, 2641, 3942
PleI GAGTC 5 cut(s) 46, 882, 1037, 1325, 3866
PmaCI CACGTG 1 cut(s) 1412
PmlI CACGTG 1 cut(s) 1412
PpsI GAGTC 5 cut(s) 46, 882, 1037, 1325, 3866
Ppu21I YACGTR 1 cut(s) 1412
PpuMI RGGWCCY 1 cut(s) 2123
PscI ACATGT 5 cut(s) 601, 1416, 1684, 3367, 4237
PshAI GACNNNNGTC 1 cut(s) 918
PshBI ATTAAT 2 cut(s) 618, 1113
Psp1406I AACGTT 1 cut(s) 1365
Psp5II RGGWCCY 1 cut(s) 2123
Psp6I CCWGG 4 cut(s) 1531, 1568, 3106, 3841
PspCI CACGTG 1 cut(s) 1412
PspFI CCCAGC 3 cut(s) 1627, 4094, 4145
PspGI CCWGG 4 cut(s) 1531, 1568, 3106, 3841
PspN4I GGNNCC 9 cut(s) 23, 325, 1676, 1785, 2019, 3351, 3681, 4151, 4315
PspPI GGNCC 9 cut(s) 561, 635, 1107, 1675, 1826, 2123, 2576, 3104, 4361
PspPPI RGGWCCY 1 cut(s) 2123
PsrI GAACNNNNNNTAC 2 cut(s) 2967, 2999
PsuI RGATCY 3 cut(s) 1471, 2226, 3575
PsyI GACNNNGTC 1 cut(s) 864
SalI GTCGAC 1 cut(s) 3413
SatI GCNGC 5 cut(s) 954, 1641, 2147, 2640, 3941
Sau96I GGNCC 9 cut(s) 561, 635, 1107, 1675, 1826, 2123, 2576, 3104, 4361
ScaI AGTACT 2 cut(s) 2099, 2877
SchI GAGTC 5 cut(s) 47, 882, 1037, 1325, 3866
ScrFI CCNGG 5 cut(s) 1533, 1570, 3108, 3219, 3843
SduI GDGCHC 6 cut(s) 423, 655, 2209, 2352, 3976, 4152
SfcI CTRYAG 1 cut(s) 719
SfuI TTCGAA 1 cut(s) 850
SinI GGWCC 5 cut(s) 635, 1826, 2123, 2576, 3104
SmiI ATTTAAAT 1 cut(s) 2868
SmlI CTYRAG 5 cut(s) 93, 752, 2141, 2994, 4218
SmoI CTYRAG 5 cut(s) 93, 752, 2141, 2994, 4218
SphI GCATGC 1 cut(s) 17
SseBI AGGCCT 1 cut(s) 2699
SsiI CCGC 4 cut(s) 84, 916, 3019, 3930
SspI AATATT 3 cut(s) 469, 556, 3997
SspMI CTAG 7 cut(s) 120, 237, 2660, 3146, 4029, 4101, 4116
StuI AGGCCT 1 cut(s) 2699
StyD4I CCNGG 5 cut(s) 1531, 1568, 3106, 3217, 3841
StyI CCWWGG 6 cut(s) 625, 631, 1847, 2030, 2957, 3683
SwaI ATTTAAAT 1 cut(s) 2868
TaiI ACGT 6 cut(s) 61, 259, 868, 1368, 1414, 3813
TaqI TCGA 8 cut(s) 850, 1207, 2392, 3326, 3364, 3388, 3414, 4298
TatI WGTACW 5 cut(s) 1808, 2097, 2754, 2875, 3861
TfiI GAWTC 5 cut(s) 1259, 1819, 3166, 3250, 3323
TscAI CASTG 7 cut(s) 745, 1809, 1918, 2317, 2690, 2937, 4188
TseFI GTSAC 6 cut(s) 971, 1151, 1412, 1564, 2054, 2740
TseI GCWGC 5 cut(s) 953, 1640, 2146, 2639, 3940
Tsp45I GTSAC 6 cut(s) 971, 1151, 1412, 1564, 2054, 2740
TspGWI ACGGA 2 cut(s) 1065, 1116
TspRI CASTG 7 cut(s) 745, 1809, 1918, 2317, 2690, 2937, 4188
Tth111I GACNNNGTC 1 cut(s) 864
Van91I CCANNNNNTGG 1 cut(s) 3428
VneI GTGCAC 2 cut(s) 651, 2205
VpaK11BI GGWCC 5 cut(s) 635, 1826, 2123, 2576, 3104
VspI ATTAAT 2 cut(s) 618, 1113
XcmI CCANNNNNNNNNTGG 1 cut(s) 2392
XmiI GTMKAC 2 cut(s) 1981, 3414
XmnI GAANNNNTTC 5 cut(s) 187, 504, 1299, 2796, 4344
XspI CTAG 7 cut(s) 120, 237, 2660, 3146, 4029, 4101, 4116
ZrmI AGTACT 2 cut(s) 2099, 2877
Zsp2I ATGCAT 2 cut(s) 1933, 3674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.