FvH4_5g25262

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000080
Physical Location & Seq
Reverse (-)
16748921 .. 16752164
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25262.t1

Sequence Viewer

Length: 1644 bp
ATGGCCGAAGACTACTCTAATTCGAGGGGGATGGACGACAGTTCTGTCAGAAACAGACTGCTTCAACATCTAAACAGAATGCTAGAACAATTTGATAAGAGCATTAGAGACTTTGATCTACCAGAAATGACAGCTGAGCAAGAAGATGGTCTACGAGTGCCGAGCTGTATTGAAGATGAACATTCTATTAATGTCCCTCAAGAAGATATTGATGCAATTGGTCGACTCAACAATGACCAAATGATGGCATTTAACACCATACTGGATGCGGTCGTGAGAAGAGAAAGTGCATTATTTTTTGTTGATGGTCCTGGAGGAACTGGGAAGACGTACCTATACCGTGCATTGTTGGCAAAGTTAAGAAGCATGAATCATATAGTCCTGGCAACTGCATCATCCGGAATAGCAGCAACTATCTTGCCCGGCGGGAGAATAGCTCACTCTAGATTCAAGATCCCACTTGATGTAAAAGATCCGACATCCATGTGTTCCATAGAAAAACAGTCTCCACTGGCACAATTGGTACGAGATTCCACTGCAATCATCTGGGATGAGGCACCTATGACAAATAGACACGCCTTTGAAGCACTTGATAGAACTTTCTGTGACATAATGGGCGTAGAAATTCCATTCGGTGGAAAAATAATGATTTTCGGGGGAGATTTTCGTCAAGTCCTTCCTGTTGTTCCTAAAGGAACAAGGTCTCAACTAATTGAAGCCTCCATTGTGAAATCAGCATTTTGGGCACAAGTAAGGATATTGAGATTGAGACAAAATATGAGATCCATAAATGACCCCCAATTTGCAGAGTTTCTACTTCGTGTTGGTGATGGGAGTGAACATGTCATCGATGACGAAATGATAAGATTACCGGAATGCATGGTGATTCCTTGGGAAAGCGAACAATCTATCACCAAAATAATTGATGATGTTTTCCCCAATTTATCAAATCATGTCAACGATGCAAGATACATGGTGGACAGAGCATTGATTACTCCTCTAAACGATGATGTGGACATGTTAAATGAGAAGATTATCAGAATGTTTCCAGGTGAAGAAATTACAGTATACTCATTTGACTTTGTGGAAGATGACACAAGAAATTTGTACCAACCAGAGTTTTTGAACTCGATCAACGCCGGTGGGTTGCCGCCCCATAAATTAACTTTGAAAACAGGTGCTCCAATCATGCTTCTAAGAAATATTGACCCAAAATTGGGATTATGTAACGGCACAAGATTGTTGTGTCACGGTTCCTACCAAAACCTGATACATGCTGAGATACTAACCGGACAATTTGCTGGGGCTCGAGTTTTTCTACCTAGGATTCCTTTGAAAAGTCAAGAAACTGCTGCGCTACCGTTTCAACTAACAAGAAAACAATTTCCAGTCAAACTGAGTTTCTCTTTGACCATAAACAAATCTCAAGGTCAGACGATCCCACAAGTAGGTGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTGTATGTGGCGTTGTCTAGAGGAGTATCGAAGTCAACCACAAAGATTATGGTTAAAAAAGGCTCCGATGTTGGTGAAGAAGGCGTGTTTACTAAGAACGTCGTATTTAAAGAAGTTTTGCTTCATTTGAACGCAAATGATATGTCGTCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

548

Amino Acids

61.25

Weight (kDa)

5.78

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 76 - 281 5.7e-75 PIF1-like helicase
Pif1_2B_dom PF21530 373 - 415 4.6e-21 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 556
AccB7I CCANNNNNTGG 2 cut(s) 244, 635
AccI GTMKAC 3 cut(s) 151, 223, 1068
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 3 cut(s) 269, 426, 1151
AclWI GGATC 4 cut(s) 448, 467, 777, 1432
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 624, 1102
AfaI GTAC 3 cut(s) 332, 525, 1109
AfiI CCNNNNNNNGG 5 cut(s) 244, 635, 1216, 1217, 1448
AflIII ACRYGT 2 cut(s) 841, 1017
AjnI CCWGG 3 cut(s) 310, 381, 1048
AluBI AGCT 3 cut(s) 134, 165, 437
AluI AGCT 3 cut(s) 134, 165, 437
Alw21I GWGCWC 1 cut(s) 1183
Alw26I GTCTC 4 cut(s) 102, 510, 708, 763
AlwI GGATC 4 cut(s) 448, 467, 777, 1432
Ama87I CYCGRG 1 cut(s) 1308
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 407, 1352
ApoI RAATTY 2 cut(s) 624, 1102
ArsI GACNNNNNNTTYG 3 cut(s) 785, 817, 1616
AseI ATTAAT 1 cut(s) 189
AspA2I CCTAGG 1 cut(s) 1322
AspLEI GCGC 1 cut(s) 1357
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 5 cut(s) 839, 895, 904, 1064, 1574
AvaI CYCGRG 1 cut(s) 1308
AvaII GGWCC 1 cut(s) 308
AvrII CCTAGG 1 cut(s) 1322
BaeGI GKGCMC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 556
BanII GRGCYC 1 cut(s) 1309
BarI GAAGNNNNNNTAC 2 cut(s) 135, 167
BbsI GAAGAC 3 cut(s) 15, 332, 1629
Bbv12I GWGCWC 1 cut(s) 1183
BbvI GCAGC 2 cut(s) 419, 1339
BccI CCATC 5 cut(s) 25, 140, 238, 299, 824
BceAI ACGGC 1 cut(s) 1246
BciT130I CCWGG 3 cut(s) 312, 383, 1050
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 4 cut(s) 102, 510, 708, 763
BfaI CTAG 4 cut(s) 83, 444, 1323, 1506
BisI GCNGC 3 cut(s) 408, 1151, 1353
BlnI CCTAGG 1 cut(s) 1322
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 3 cut(s) 409, 1152, 1354
Bme1390I CCNGG 4 cut(s) 312, 383, 423, 1050
Bme18I GGWCC 1 cut(s) 308
BmeT110I CYCGRG 1 cut(s) 1308
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 3 cut(s) 558, 1255, 1552
BmrFI CCNGG 4 cut(s) 312, 383, 423, 1050
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 4 cut(s) 202, 256, 401, 952
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 3 cut(s) 15, 332, 1629
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 1 cut(s) 333
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 183, 1410
BpuMI CCSGG 1 cut(s) 423
Bsa29I ATCGAT 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 2 cut(s) 890, 1322
BsaWI WCCGGW 3 cut(s) 398, 871, 1289
BsaXI ACNNNNNCTCC 2 cut(s) 1165, 1195
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 635, 1216, 1217, 1448
Bse118I RCCGGY 1 cut(s) 1139
Bse1I ACTGG 4 cut(s) 267, 325, 516, 1388
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 3 cut(s) 312, 383, 1050
BseCI ATCGAT 1 cut(s) 849
BseDI CCNNGG 2 cut(s) 890, 1322
BseGI GGATG 5 cut(s) 36, 271, 395, 479, 556
BseLI CCNNNNNNNGG 5 cut(s) 244, 635, 1216, 1217, 1448
BseMII CTCAG 3 cut(s) 126, 1269, 1388
BseNI ACTGG 4 cut(s) 267, 325, 516, 1388
BseRI GAGGAG 2 cut(s) 987, 1524
BseSI GKGCMC 1 cut(s) 748
BseXI GCAGC 2 cut(s) 419, 1339
BseYI CCCAGC 1 cut(s) 1301
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 556
BshVI ATCGAT 1 cut(s) 849
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 1183
BsiHKCI CYCGRG 1 cut(s) 1308
BsiSI CCGG 5 cut(s) 399, 423, 872, 1140, 1290
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 5 cut(s) 244, 635, 1216, 1217, 1448
BsmAI GTCTC 4 cut(s) 102, 510, 708, 763
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 2 cut(s) 84, 881
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 1 cut(s) 1308
Bsp1286I GDGCHC 3 cut(s) 748, 1183, 1309
Bsp13I TCCGGA 1 cut(s) 398
Bsp143I GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 3 cut(s) 269, 426, 1151
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 127, 1270, 1389
BspDI ATCGAT 1 cut(s) 849
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 3 cut(s) 558, 1255, 1552
BspPI GGATC 4 cut(s) 448, 467, 777, 1432
BspT107I GGYRCC 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 1139
BsrI ACTGG 4 cut(s) 267, 325, 516, 1388
BssAI RCCGGY 1 cut(s) 1139
BssECI CCNNGG 2 cut(s) 890, 1322
BssMI GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
BssNAI GTATAC 1 cut(s) 1069
BssT1I CCWWGG 2 cut(s) 890, 1322
Bst1107I GTATAC 1 cut(s) 1069
Bst2UI CCWGG 3 cut(s) 312, 383, 1050
Bst4CI ACNGT 6 cut(s) 41, 341, 504, 1066, 1253, 1362
Bst6I CTCTTC 1 cut(s) 274
BstDEI CTNAG 5 cut(s) 135, 1196, 1278, 1397, 1581
BstF5I GGATG 5 cut(s) 36, 271, 395, 479, 556
BstHHI GCGC 1 cut(s) 1357
BstKTI GATC 7 cut(s) 118, 456, 475, 785, 1134, 1440, 1468
BstMAI GTCTC 4 cut(s) 102, 510, 708, 763
BstMBI GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 3 cut(s) 350, 584, 743
BstNI CCWGG 3 cut(s) 312, 383, 1050
BstNSI RCATGY 3 cut(s) 845, 1021, 1277
BstSCI CCNGG 4 cut(s) 310, 381, 421, 1048
BstSLI GKGCMC 1 cut(s) 748
BstV1I GCAGC 2 cut(s) 419, 1339
BstV2I GAAGAC 3 cut(s) 15, 332, 1629
BstX2I RGATCY 3 cut(s) 453, 472, 782
BstYI RGATCY 3 cut(s) 453, 472, 782
BstZ17I GTATAC 1 cut(s) 1069
Bsu15I ATCGAT 1 cut(s) 849
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 849
BtsCI GGATG 5 cut(s) 36, 271, 395, 479, 556
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 509, 534
CfoI GCGC 1 cut(s) 1357
Cfr10I RCCGGY 1 cut(s) 1139
Cfr13I GGNCC 1 cut(s) 308
ClaI ATCGAT 1 cut(s) 849
Csp6I GTAC 3 cut(s) 331, 524, 1108
CspCI CAANNNNNGTGG 2 cut(s) 1123, 1158
CviJI RGCY 7 cut(s) 5, 134, 165, 437, 719, 1307, 1551
CviKI_1 RGCY 7 cut(s) 5, 134, 165, 437, 719, 1307, 1551
CviQI GTAC 3 cut(s) 331, 524, 1108
DdeI CTNAG 5 cut(s) 135, 1196, 1278, 1397, 1581
DpnI GATC 7 cut(s) 117, 455, 474, 784, 1133, 1439, 1467
DpnII GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
DraI TTTAAA 1 cut(s) 1597
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 274
Eco130I CCWWGG 2 cut(s) 890, 1322
Eco24I GRGCYC 1 cut(s) 1309
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 1308
EcoRII CCWGG 3 cut(s) 310, 381, 1048
EcoT14I CCWWGG 2 cut(s) 890, 1322
EcoT22I ATGCAT 1 cut(s) 881
EcoT38I GRGCYC 1 cut(s) 1309
ErhI CCWWGG 2 cut(s) 890, 1322
FalI AAGNNNNNCTT 2 cut(s) 1593, 1625
FaqI GGGAC 1 cut(s) 179
FauI CCCGC 1 cut(s) 419
FblI GTMKAC 3 cut(s) 151, 223, 1068
Fnu4HI GCNGC 3 cut(s) 408, 1151, 1353
FokI GGATG 5 cut(s) 43, 278, 382, 466, 563
FriOI GRGCYC 1 cut(s) 1309
Fsp4HI GCNGC 3 cut(s) 408, 1151, 1353
FspBI CTAG 4 cut(s) 83, 444, 1323, 1506
GlaI GCGC 1 cut(s) 1356
GluI GCNGC 3 cut(s) 408, 1151, 1353
GsaI CCCAGC 1 cut(s) 1305
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 1 cut(s) 5
HapII CCGG 5 cut(s) 399, 423, 872, 1140, 1290
HhaI GCGC 1 cut(s) 1357
Hin6I GCGC 1 cut(s) 1355
HinP1I GCGC 1 cut(s) 1355
HincII GTYRAC 3 cut(s) 224, 958, 1524
HindII GTYRAC 3 cut(s) 224, 958, 1524
HinfI GANTC 6 cut(s) 225, 370, 447, 530, 886, 1327
HpaII CCGG 5 cut(s) 399, 423, 872, 1140, 1290
HphI GGTGA 5 cut(s) 839, 895, 904, 1064, 1574
Hpy188I TCNGA 5 cut(s) 50, 477, 1040, 1434, 1555
Hpy188III TCNNGA 7 cut(s) 200, 274, 399, 444, 451, 1343, 1506
Hpy99I CGWCG 1 cut(s) 1592
HpyAV CCTTC 2 cut(s) 686, 1562
HpyCH4III ACNGT 6 cut(s) 41, 341, 504, 1066, 1253, 1362
HpyCH4IV ACGT 2 cut(s) 329, 1587
HpyCH4V TGCA 8 cut(s) 215, 290, 344, 392, 539, 806, 879, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 584, 743
HpyF3I CTNAG 5 cut(s) 135, 1196, 1278, 1397, 1581
HpySE526I ACGT 2 cut(s) 329, 1587
HspAI GCGC 1 cut(s) 1355
Kpn2I TCCGGA 1 cut(s) 398
Kzo9I GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
LmnI GCTCC 2 cut(s) 1186, 1556
Lsp1109I GCAGC 2 cut(s) 419, 1339
LweI GCATC 4 cut(s) 202, 256, 401, 952
MaeI CTAG 4 cut(s) 83, 444, 1323, 1506
MaeII ACGT 2 cut(s) 329, 1587
MaeIII GTNAC 3 cut(s) 605, 1226, 1247
MalI GATC 7 cut(s) 117, 455, 474, 784, 1133, 1439, 1467
MboI GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
MfeI CAATTG 3 cut(s) 216, 518, 1487
MflI RGATCY 3 cut(s) 453, 472, 782
MhlI GDGCHC 3 cut(s) 748, 1183, 1309
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 500
MnlI CCTC 8 cut(s) 18, 207, 308, 547, 730, 1008, 1469, 1502
Mph1103I ATGCAT 1 cut(s) 881
MroI TCCGGA 1 cut(s) 398
MseI TTAA 7 cut(s) 189, 252, 359, 1022, 1163, 1542, 1596
MslI CAYNNNNRTG 1 cut(s) 484
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 5 cut(s) 399, 423, 872, 1140, 1290
MspR9I CCNGG 4 cut(s) 312, 383, 423, 1050
MunI CAATTG 3 cut(s) 216, 518, 1487
Mva1269I GAATGC 2 cut(s) 84, 881
MvaI CCWGG 3 cut(s) 312, 383, 1050
MwoI GCNNNNNNNGC 3 cut(s) 350, 584, 743
NciI CCSGG 1 cut(s) 423
NdeII GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
NlaIV GGNNCC 3 cut(s) 558, 1255, 1552
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 2 cut(s) 605, 1247
NsiI ATGCAT 1 cut(s) 881
NspI RCATGY 3 cut(s) 845, 1021, 1277
PaeR7I CTCGAG 1 cut(s) 1308
PciI ACATGT 2 cut(s) 841, 1017
PctI GAATGC 2 cut(s) 84, 881
PfeI GAWTC 5 cut(s) 370, 447, 530, 886, 1327
PflMI CCANNNNNTGG 2 cut(s) 244, 635
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 3 cut(s) 409, 1152, 1354
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PscI ACATGT 2 cut(s) 841, 1017
PshBI ATTAAT 1 cut(s) 189
Psp6I CCWGG 3 cut(s) 310, 381, 1048
PspFI CCCAGC 1 cut(s) 1301
PspGI CCWGG 3 cut(s) 310, 381, 1048
PspN4I GGNNCC 3 cut(s) 558, 1255, 1552
PspPI GGNCC 1 cut(s) 308
PspXI VCTCGAGB 1 cut(s) 1308
PsuI RGATCY 3 cut(s) 453, 472, 782
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 3 cut(s) 332, 525, 1109
RsaNI GTAC 3 cut(s) 331, 524, 1108
RseI CAYNNNNRTG 1 cut(s) 484
SalI GTCGAC 1 cut(s) 222
SaqAI TTAA 7 cut(s) 189, 252, 359, 1022, 1163, 1542, 1596
SatI GCNGC 3 cut(s) 408, 1151, 1353
Sau3AI GATC 7 cut(s) 115, 453, 472, 782, 1131, 1437, 1465
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 4 cut(s) 312, 383, 423, 1050
SduI GDGCHC 3 cut(s) 748, 1183, 1309
SfaNI GCATC 4 cut(s) 202, 256, 401, 952
Sfr274I CTCGAG 1 cut(s) 1308
SgrAI CRCCGGYG 1 cut(s) 1139
SinI GGWCC 1 cut(s) 308
SlaI CTCGAG 1 cut(s) 1308
SmiMI CAYNNNNRTG 1 cut(s) 484
SmlI CTYRAG 3 cut(s) 198, 1308, 1425
SmoI CTYRAG 3 cut(s) 198, 1308, 1425
SsiI CCGC 3 cut(s) 269, 426, 1151
SspI AATATT 1 cut(s) 1204
SspMI CTAG 4 cut(s) 83, 444, 1323, 1506
StyD4I CCNGG 4 cut(s) 310, 381, 421, 1048
StyI CCWWGG 2 cut(s) 890, 1322
TaaI ACNGT 6 cut(s) 41, 341, 504, 1066, 1253, 1362
TaiI ACGT 2 cut(s) 332, 1590
TaqI TCGA 6 cut(s) 23, 223, 849, 1130, 1309, 1517
TauI GCSGC 1 cut(s) 1153
TfiI GAWTC 5 cut(s) 370, 447, 530, 886, 1327
Tru1I TTAA 7 cut(s) 189, 252, 359, 1022, 1163, 1542, 1596
Tru9I TTAA 7 cut(s) 189, 252, 359, 1022, 1163, 1542, 1596
TscAI CASTG 2 cut(s) 516, 541
TseFI GTSAC 2 cut(s) 605, 1247
TseI GCWGC 2 cut(s) 407, 1352
Tsp45I GTSAC 2 cut(s) 605, 1247
TspDTI ATGAA 4 cut(s) 192, 383, 1601, 1629
TspRI CASTG 2 cut(s) 516, 541
Van91I CCANNNNNTGG 2 cut(s) 244, 635
VpaK11BI GGWCC 1 cut(s) 308
VspI ATTAAT 1 cut(s) 189
XapI RAATTY 2 cut(s) 624, 1102
XbaI TCTAGA 2 cut(s) 443, 1505
XceI RCATGY 3 cut(s) 845, 1021, 1277
XcmI CCANNNNNNNNNTGG 1 cut(s) 1534
XhoI CTCGAG 1 cut(s) 1308
XmaJI CCTAGG 1 cut(s) 1322
XmiI GTMKAC 3 cut(s) 151, 223, 1068
XspI CTAG 4 cut(s) 83, 444, 1323, 1506
Zsp2I ATGCAT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.