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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 105 |
| AccI | GTMKAC | 1 cut(s) | 16 |
| AciI | CCGC | 2 cut(s) | 12, 25 |
| AcuI | CTGAAG | 1 cut(s) | 231 |
| AluBI | AGCT | 1 cut(s) | 58 |
| AluI | AGCT | 1 cut(s) | 58 |
| Alw21I | GWGCWC | 1 cut(s) | 60 |
| Alw26I | GTCTC | 1 cut(s) | 134 |
| ApeKI | GCWGC | 1 cut(s) | 301 |
| AseI | ATTAAT | 2 cut(s) | 33, 44 |
| AspLEI | GCGC | 1 cut(s) | 130 |
| AsuC2I | CCSGG | 1 cut(s) | 125 |
| BanII | GRGCYC | 1 cut(s) | 60 |
| BbsI | GAAGAC | 1 cut(s) | 102 |
| Bbv12I | GWGCWC | 1 cut(s) | 60 |
| BbvI | GCAGC | 1 cut(s) | 288 |
| BccI | CCATC | 1 cut(s) | 142 |
| BclI | TGATCA | 1 cut(s) | 181 |
| BcnI | CCSGG | 1 cut(s) | 125 |
| BcoDI | GTCTC | 1 cut(s) | 134 |
| BfmI | CTRYAG | 1 cut(s) | 100 |
| BfuAI | ACCTGC | 1 cut(s) | 105 |
| BglII | AGATCT | 1 cut(s) | 82 |
| BisI | GCNGC | 2 cut(s) | 26, 302 |
| BlsI | GCNGC | 2 cut(s) | 27, 303 |
| Bme1390I | CCNGG | 1 cut(s) | 125 |
| BmrFI | CCNGG | 1 cut(s) | 125 |
| BpiI | GAAGAC | 1 cut(s) | 102 |
| BpuMI | CCSGG | 1 cut(s) | 125 |
| BsaAI | YACGTR | 1 cut(s) | 293 |
| Bse3DI | GCAATG | 1 cut(s) | 184 |
| BseMI | GCAATG | 1 cut(s) | 184 |
| BseMII | CTCAG | 1 cut(s) | 129 |
| BseXI | GCAGC | 1 cut(s) | 288 |
| BsiHKAI | GWGCWC | 1 cut(s) | 60 |
| BsiSI | CCGG | 1 cut(s) | 124 |
| BsmAI | GTCTC | 1 cut(s) | 134 |
| Bsp1286I | GDGCHC | 1 cut(s) | 60 |
| Bsp143I | GATC | 4 cut(s) | 61, 82, 147, 181 |
| BspACI | CCGC | 2 cut(s) | 12, 25 |
| BspCNI | CTCAG | 1 cut(s) | 130 |
| BspMI | ACCTGC | 1 cut(s) | 105 |
| BsrDI | GCAATG | 1 cut(s) | 184 |
| BssMI | GATC | 4 cut(s) | 61, 82, 147, 181 |
| Bst4CI | ACNGT | 1 cut(s) | 273 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 120 |
| BstBAI | YACGTR | 1 cut(s) | 293 |
| BstDEI | CTNAG | 2 cut(s) | 20, 138 |
| BstHHI | GCGC | 1 cut(s) | 130 |
| BstKTI | GATC | 4 cut(s) | 64, 85, 150, 184 |
| BstMAI | GTCTC | 1 cut(s) | 134 |
| BstMBI | GATC | 4 cut(s) | 61, 82, 147, 181 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 120 |
| BstSCI | CCNGG | 1 cut(s) | 123 |
| BstSFI | CTRYAG | 1 cut(s) | 100 |
| BstV1I | GCAGC | 1 cut(s) | 288 |
| BstV2I | GAAGAC | 1 cut(s) | 102 |
| BstX2I | RGATCY | 1 cut(s) | 82 |
| BstYI | RGATCY | 1 cut(s) | 82 |
| BtsIMutI | CAGTG | 2 cut(s) | 143, 278 |
| BveI | ACCTGC | 1 cut(s) | 105 |
| CfoI | GCGC | 1 cut(s) | 130 |
| CviAII | CATG | 1 cut(s) | 205 |
| CviJI | RGCY | 3 cut(s) | 28, 58, 237 |
| CviKI_1 | RGCY | 3 cut(s) | 28, 58, 237 |
| DdeI | CTNAG | 2 cut(s) | 20, 138 |
| DpnI | GATC | 4 cut(s) | 63, 84, 149, 183 |
| DpnII | GATC | 4 cut(s) | 61, 82, 147, 181 |
| Ecl136II | GAGCTC | 1 cut(s) | 58 |
| Eco24I | GRGCYC | 1 cut(s) | 60 |
| Eco53kI | GAGCTC | 1 cut(s) | 58 |
| Eco57I | CTGAAG | 1 cut(s) | 231 |
| EcoICRI | GAGCTC | 1 cut(s) | 58 |
| EcoT38I | GRGCYC | 1 cut(s) | 60 |
| FaeI | CATG | 1 cut(s) | 208 |
| FatI | CATG | 1 cut(s) | 204 |
| FbaI | TGATCA | 1 cut(s) | 181 |
| FblI | GTMKAC | 1 cut(s) | 16 |
| Fnu4HI | GCNGC | 2 cut(s) | 26, 302 |
| FriOI | GRGCYC | 1 cut(s) | 60 |
| Fsp4HI | GCNGC | 2 cut(s) | 26, 302 |
| GlaI | GCGC | 1 cut(s) | 129 |
| GluI | GCNGC | 2 cut(s) | 26, 302 |
| HapII | CCGG | 1 cut(s) | 124 |
| HhaI | GCGC | 1 cut(s) | 130 |
| Hin1II | CATG | 1 cut(s) | 208 |
| Hin6I | GCGC | 1 cut(s) | 128 |
| HinP1I | GCGC | 1 cut(s) | 128 |
| HpaII | CCGG | 1 cut(s) | 124 |
| Hpy166II | GTNNAC | 1 cut(s) | 17 |
| Hpy188I | TCNGA | 2 cut(s) | 139, 286 |
| Hpy8I | GTNNAC | 1 cut(s) | 17 |
| HpyCH4III | ACNGT | 1 cut(s) | 273 |
| HpyCH4IV | ACGT | 1 cut(s) | 292 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 120 |
| HpyF3I | CTNAG | 2 cut(s) | 20, 138 |
| HpySE526I | ACGT | 1 cut(s) | 292 |
| Hsp92II | CATG | 1 cut(s) | 208 |
| HspAI | GCGC | 1 cut(s) | 128 |
| Ksp22I | TGATCA | 1 cut(s) | 181 |
| Kzo9I | GATC | 4 cut(s) | 61, 82, 147, 181 |
| LpnPI | CCDG | 3 cut(s) | 71, 100, 137 |
| Lsp1109I | GCAGC | 1 cut(s) | 288 |
| MaeII | ACGT | 1 cut(s) | 292 |
| MalI | GATC | 4 cut(s) | 63, 84, 149, 183 |
| MboI | GATC | 4 cut(s) | 61, 82, 147, 181 |
| MboII | GAAGA | 2 cut(s) | 107, 224 |
| MflI | RGATCY | 1 cut(s) | 82 |
| MhlI | GDGCHC | 1 cut(s) | 60 |
| MluCI | AATT | 3 cut(s) | 30, 34, 65 |
| MseI | TTAA | 3 cut(s) | 33, 44, 68 |
| MslI | CAYNNNNRTG | 1 cut(s) | 44 |
| MspI | CCGG | 1 cut(s) | 124 |
| MspR9I | CCNGG | 1 cut(s) | 125 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 120 |
| NciI | CCSGG | 1 cut(s) | 125 |
| NdeII | GATC | 4 cut(s) | 61, 82, 147, 181 |
| NlaIII | CATG | 1 cut(s) | 208 |
| PkrI | GCNGC | 2 cut(s) | 27, 303 |
| Ppu21I | YACGTR | 1 cut(s) | 293 |
| PshBI | ATTAAT | 2 cut(s) | 33, 44 |
| Psp124BI | GAGCTC | 1 cut(s) | 60 |
| PsuI | RGATCY | 1 cut(s) | 82 |
| RseI | CAYNNNNRTG | 1 cut(s) | 44 |
| SacI | GAGCTC | 1 cut(s) | 60 |
| SaqAI | TTAA | 3 cut(s) | 33, 44, 68 |
| SatI | GCNGC | 2 cut(s) | 26, 302 |
| Sau3AI | GATC | 4 cut(s) | 61, 82, 147, 181 |
| ScrFI | CCNGG | 1 cut(s) | 125 |
| SduI | GDGCHC | 1 cut(s) | 60 |
| SetI | ASST | 3 cut(s) | 60, 119, 295 |
| SfcI | CTRYAG | 1 cut(s) | 100 |
| SgeI | CNNG | 8 cut(s) | 71, 98, 127, 136, 137, 217, 271, 303 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 44 |
| Sse9I | AATT | 3 cut(s) | 30, 34, 65 |
| SsiI | CCGC | 2 cut(s) | 12, 25 |
| SstI | GAGCTC | 1 cut(s) | 60 |
| StyD4I | CCNGG | 1 cut(s) | 123 |
| TaaI | ACNGT | 1 cut(s) | 273 |
| TaiI | ACGT | 1 cut(s) | 295 |
| TaqI | TCGA | 1 cut(s) | 60 |
| TasI | AATT | 3 cut(s) | 30, 34, 65 |
| TauI | GCSGC | 1 cut(s) | 28 |
| Tru1I | TTAA | 3 cut(s) | 33, 44, 68 |
| Tru9I | TTAA | 3 cut(s) | 33, 44, 68 |
| TscAI | CASTG | 2 cut(s) | 150, 278 |
| TseI | GCWGC | 1 cut(s) | 301 |
| TspRI | CASTG | 2 cut(s) | 150, 278 |
| VspI | ATTAAT | 2 cut(s) | 33, 44 |
| XmiI | GTMKAC | 1 cut(s) | 16 |